Jill A. Harned

Works (14)

Updated: July 15th, 2023 21:16

2016 journal article

Evaluation of the proliferative capacity of canine retinal pigment epithelial cells harvested from different regions of the fundus

Investigative Ophthalmology and Visual Science, 57(12).

By: F. Mowat, J. Hash, P. Mzyk, J. Harned, S. Nagar & M. McGahan

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Hypoxia controls iron metabolism and glutamate secretion in retinal pigmented epithelial cells

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1840(10), 3138–3144.

By: J. Harned n, S. Nagar n & M. McGahan  n

author keywords: Iron transport; Glutamate; Hypoxia; Blood-ocular barriers
MeSH headings : Animals; Cell Hypoxia; Cells, Cultured; Dogs; Epithelial Cells / metabolism; Epithelial Cells / pathology; Glutamic Acid / metabolism; Humans; Iron / metabolism; Oxidation-Reduction; Retinal Pigment Epithelium / metabolism; Retinal Pigment Epithelium / pathology; Stroke / metabolism; Stroke / pathology
TL;DR: Iron, a key catalyst of free radical generation, is removed from RPE under hypoxic conditions which may help protect RPE from oxidative stress and indicate the importance of using polarized tight junctional cells as more physiologically relevant models for blood-barrier-like systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Hydrogen peroxide and extracellular signal-related kinase 1/2 pathway regulate ferritin levels in retinal pigmented and lens epithelial cells

Molecular Vision, 19, 2106–2112.

By: M. Lall, J. Harned & M. McGahan

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Ceruloplasmin alters intracellular iron regulated proteins and pathways: Ferritin, transferrin receptor, glutamate and hypoxia-inducible factor-1α

Experimental Eye Research, 97(1), 90–97.

By: J. Harned n, J. Ferrell n, S. Nagar n, M. Goralska n, L. Fleisher n & M. McGahan  n

author keywords: ceruloplasmin; iron; lens epithelial cells; retinal pigmented epithelial cells glutamate; hypoxia-inducible factor; transferrin receptor; ferritin
MeSH headings : Animals; Cells, Cultured; Ceruloplasmin / metabolism; Ceruloplasmin / pharmacology; Dogs; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Ferritins / metabolism; Fluorescent Antibody Technique, Indirect; Glutamic Acid / metabolism; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; Iron Chelating Agents / pharmacology; Lens, Crystalline / cytology; Oxalates / pharmacology; Receptors, Transferrin / metabolism; Retinal Pigment Epithelium / drug effects; Retinal Pigment Epithelium / metabolism
TL;DR: The Cp increased production and secretion of the neurotransmitter, glutamate, is a substantive finding of clinical relevance because of the neural and retinal degeneration found in aceruloplasminemia patients. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref
Added: August 6, 2018

2010 journal article

Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 51(9), 4437–4446.

By: J. Harned n, J. Ferrell n, M. Lall n, L. Fleisher n, S. Nagar n, M. Goralska n, M. McGahan  n

MeSH headings : Animals; Apoferritins / genetics; Apoferritins / metabolism; Cell Nucleus / metabolism; Cells, Cultured; Culture Media, Conditioned / pharmacology; Cystine / pharmacokinetics; Dogs; Epithelial Cells / cytology; Epithelial Cells / metabolism; Glutathione / metabolism; Homeostasis / physiology; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; Iron / metabolism; Lens, Crystalline / cytology; Lens, Crystalline / metabolism; Oxidative Stress / physiology; Protein Subunits / genetics; Protein Subunits / metabolism; RNA, Small Interfering; Transfection; Vascular Endothelial Growth Factor A / metabolism
TL;DR: Iron storage in ferritin was greatly reduced by FHsiRNA, resulting in increased iron availability, as noted by a decrease in transferrin receptor levels and iron uptake from transferrin, and profound effects clearly demonstrate that maintenance of a specific H:L ratio is part of a basic cellular homeostatic mechanism. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Iron metabolism in the eye: A review

Experimental Eye Research, 88(2), 204–215.

By: M. Goralska n, J. Ferrell n, J. Harned n, M. Lall n, S. Nagar n, L. Fleisher n, M. McGahan  n

author keywords: iron; ferritin; transferrin; lens; retinal pigmented epithelium; retina
MeSH headings : Animals; Eye / metabolism; Eye Diseases / metabolism; Homeostasis; Humans; Iron / metabolism; Oxidative Stress; Signal Transduction / physiology
TL;DR: This review article covers all aspects of iron metabolism, which include studies of iron levels within the eye and the processes used to maintain normal levels of iron in ocular tissues. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref
Added: August 6, 2018

2006 journal article

Lens epithelial cells synthesize and secrete ceruloplasmin: Effects of ceruloplasmin and transferrin on iron efflux and intracellular iron dynamics

EXPERIMENTAL EYE RESEARCH, 83(4), 721–727.

By: J. Harned n, L. Fleisher n & M. McGahan  n

author keywords: lens; ceruloplasmin; iron; ferritin; transferrin; labile iron pool
MeSH headings : Animals; Ceruloplasmin / biosynthesis; Ceruloplasmin / pharmacology; Ceruloplasmin / physiology; Culture Media, Conditioned; Cytosol / metabolism; Dogs; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Ferritins / metabolism; Iron / metabolism; Lens Capsule, Crystalline / cytology; Lens Capsule, Crystalline / drug effects; Lens Capsule, Crystalline / metabolism; Tissue Culture Techniques; Transferrin / biosynthesis; Transferrin / pharmacology; Transferrin / physiology
TL;DR: An important role for ceruloplasmin and transferrin in iron metabolism in the lens is indicated, with results consistent with increased efflux of iron. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Iron alters glutamate secretion by regulating cytosolic aconitase activity

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 288(5), C1117–C1124.

By: M. McGahan  n, J. Harned n, M. Mukunnemkeril n, M. Goralska n, L. Fleisher n & J. Ferrell n

author keywords: retinal pigment epithelial cells; lens epithelial cells
MeSH headings : Aconitate Hydratase / antagonists & inhibitors; Aconitate Hydratase / metabolism; Animals; Cells, Cultured; Citric Acid / metabolism; Cytosol / metabolism; Dogs; Enzyme Activation / drug effects; Glutamates / metabolism; Iron / metabolism; Isocitrates / metabolism; Membrane Transport Proteins / metabolism; Oxalates / pharmacology; Pigment Epithelium of Eye / drug effects; Pigment Epithelium of Eye / enzymology; Riluzole / pharmacology; Vesicular Glutamate Transport Protein 1
TL;DR: The results are the first to provide a functional link between these two physiologically important substances by demonstrating a significant role for iron in the regulation of glutamate production and secretion in mammalian cells resulting from iron regulation of aconitase activity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

The effect of UVB irradiation on ferritin subunit synthesis, ferritin assembly and Fe metabolism in cultured canine lens epithelial cells

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 77(4), 440–445.

By: J. Harned*, A. Grimes* & M. McGahan

TL;DR: It was hypothesized and then determined that newly synthesized chains did not assemble into functional holoferritin, and this study provides a cautionary note regarding the conclusions of such studies in the absence of data indicating assembly of functional ferritin molecules. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

The effect of ascorbic acid and ferric ammonium citrate on iron uptake and storage in lens epithelial cells

EXPERIMENTAL EYE RESEARCH, 66(6), 687–697.

By: M. Goralska n, J. Harned n, L. Fleisher n & M. McGahan  n

author keywords: ascorbic acid; ferritin; lens; iron; ferric ammonium citrate; transferrin
MeSH headings : Animals; Apoferritins / biosynthesis; Ascorbic Acid / pharmacology; Cells, Cultured; Chlorides; Dogs; Epithelial Cells / metabolism; Ferric Compounds / metabolism; Ferric Compounds / pharmacology; Homeostasis; Humans; Infant, Newborn; Iron Radioisotopes / metabolism; Lens, Crystalline / metabolism; Quaternary Ammonium Compounds / pharmacology; Transferrin / metabolism
TL;DR: Iron homeostasis in lens epithelial cells is affected mainly by changes in apoferritin synthesis, which is greatly stimulated by ascorbic acid, rather than by altering the rate of protein degradation, which has been very slow in these cells under all circumstances. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Transferrin in after-cataract and as a survival factor for lens epithelium

EXPERIMENTAL EYE RESEARCH, 66(2), 207–215.

By: M. Davidson n, J. Harned n, A. Grimes n, G. Duncan*, I. Wormstone * & M. McGahan  n

author keywords: transferrin; after-cataract; human; rabbit; lens; epithelial cells; growth factors
MeSH headings : Animals; Cataract / pathology; Cataract Extraction; Cell Division; Cell Survival; Cells, Cultured; Epithelial Cells / metabolism; Humans; Lens Capsule, Crystalline / metabolism; Lens Capsule, Crystalline / pathology; Lens, Crystalline / metabolism; Lens, Crystalline / pathology; Postoperative Period; Rabbits; Recurrence; Transferrin / biosynthesis; Transferrin / metabolism
TL;DR: Tf acts as a survival factor for lens epithelia and its synthesis is up-regulated in after-cataract lens sacs, suggesting that Tf may play an important role in the pathogenesis of lens epithelial cell proliferation and after-Cataract formation following cataract surgery. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Mechanisms by which ascorbic acid increases ferritin levels in cultured lens epithelial cells

EXPERIMENTAL EYE RESEARCH, 64(3), 413–421.

By: M. Goralska n, J. Harned n, A. Grimes n, L. Fleisher n & M. McGahan  n

author keywords: ascorbic acid; ferritin; lens; cataract
MeSH headings : Animals; Ascorbic Acid / metabolism; Ascorbic Acid / pharmacology; Base Sequence; Blotting, Northern; Cells, Cultured; DNA / metabolism; Deferoxamine / pharmacology; Dithiothreitol / pharmacology; Dogs; Epithelium / drug effects; Epithelium / metabolism; Ferric Compounds / metabolism; Ferritins / biosynthesis; Ferritins / drug effects; Ferritins / genetics; Hydrogen Peroxide / pharmacology; Lens, Crystalline / drug effects; Lens, Crystalline / metabolism; Molecular Sequence Data; Oxidation-Reduction; Protein Biosynthesis; Protein Synthesis Inhibitors / pharmacology; RNA, Messenger / drug effects; RNA, Messenger / metabolism
TL;DR: The ability of ascorbic acid to increase ferritin concentration in lens epithelial cells could provide an additional protective mechanism for this antioxidant vitamin. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1995 journal article

TRANSFERRIN SECRETION BY LENS EPITHELIAL-CELLS IN CULTURE

EXPERIMENTAL EYE RESEARCH, 60(6), 667–673.

By: M. Mcgahan  n, J. Harned n, M. Goralska n, B. Sherry n & L. Fleisher n

author keywords: TRANSFERRIN; LENS; ANTIOXIDANTS; GROWTH FACTORS; AQUEOUS HUMOR
MeSH headings : Animals; Aqueous Humor / chemistry; Blotting, Northern; Cells, Cultured; Ciliary Body / metabolism; Dogs; Epithelium / metabolism; Lens, Crystalline / metabolism; Rabbits; Secretory Rate; Time Factors; Transferrin / metabolism; Vitreous Body / chemistry
TL;DR: This is the first experimental demonstration that an intraocular tissue can make and secrete Tf, and transferrin secretion by the lens may contribute significantly to the IOF content of this important intraocular protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

1994 journal article

REGULATION OF FERRITIN LEVELS IN CULTURED LENS EPITHELIAL-CELLS

EXPERIMENTAL EYE RESEARCH, 59(5), 551–555.

By: M. Mcgahan  n, J. Harned n, A. Grimes n & L. Fleisher n

author keywords: IRON; FERRITIN; LENS EPITHELIAL CELLS; PEROXIDE; FREE RADICALS; ASCORBIC ACID
MeSH headings : Animals; Cells, Cultured; Chelating Agents / pharmacology; Deferoxamine / pharmacology; Dogs; Enzyme-Linked Immunosorbent Assay; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Ferritins / metabolism; Hemin / pharmacology; Lens, Crystalline / cytology; Lens, Crystalline / drug effects; Lens, Crystalline / metabolism
TL;DR: Regulation of ferritin levels may represent a mechanism by which the lens epithelium is protected from oxidative damage, and in pathological circumstances, the iron content and redox state of the aqueous humor is dramatically altered and may affect the steady state levels of Ferritin within the lens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

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