@article{enomoto_castro_hash_thomson_nakanishi-hester_perry_aker_haupt_opperman_roe_et al._2024, title={Prevalence of radiographic appendicular osteoarthritis and associated clinical signs in young dogs}, volume={14}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-024-52324-9}, abstractNote={AbstractThis study aimed to determine the prevalence of osteoarthritis (OA) and associated clinical signs in young dogs. Owners of dogs aged 8 months–4 years from a single practice, were contacted in random order, to participate in a general health screen. Clinical and orthopedic examinations were performed. Each joint was scored for pain reactions (0–4). Orthogonal radiographs of all joints were made under sedation. Each joint was scored for radiographic OA (rOA) severity on an 11-point scale. Clinical OA (cOA) was defined as an overlap of rOA and joint pain in ≥ 1 joint. Owners completed OA questionnaires. The owners of 123 dogs agreed to participate. Overall, 39.8% (49/123) of dogs had rOA in ≥ 1 joint, and 16.3% (20/123) or 23.6% (29/123) dogs had cOA, depending on the cut-off value of joint pain; moderate (2), or mild (1), respectively. Owners of dogs with cOA observed signs of impairment in approximately 30% of cases. Only 2 dogs with cOA were receiving OA pain management. The most commonly affected joints in descending order of frequency were elbow, hip, tarsus, and stifle. Radiographically visible OA is common in young dogs, and 40–60% of dogs with rOA had cOA. However, OA-pain appears underdiagnosed and undertreated in young dogs.}, number={1}, journal={SCIENTIFIC REPORTS}, author={Enomoto, Masataka and Castro, Nicholas and Hash, Jonathan and Thomson, Andrea and Nakanishi-Hester, Aoi and Perry, Erin and Aker, Savannah and Haupt, Emily and Opperman, Logan and Roe, Simon and et al.}, year={2024}, month={Feb} }
 @article{ortiz_belda_hash_enomoto_robertson_lascelles_2022, title={Initial exploration of the discriminatory ability of the PetPace collar to detect differences in activity and physiological variables between healthy and osteoarthritic dogs}, volume={3}, ISSN={["2673-561X"]}, url={http://dx.doi.org/10.3389/fpain.2022.949877}, DOI={10.3389/fpain.2022.949877}, abstractNote={BackgroundAccelerometry has been used to evaluate activity in dogs with osteoarthritis (OA) pain, especially in relation to effect of treatment; however no studies have compared accelerometry-measured activity in dogs with OA-pain and healthy dogs. The aims of this study were to (1) compare activity output from the PetPace collar with the validated Actical monitor and (2) determine if PetPace collar outputs (overall activity, activity levels, body position, and vital signs) differed between healthy dogs and dogs with OA-pain.MethodsThis was an observational, non-interventional study in healthy dogs and dogs with OA-pain. All dogs were outfitted with the PetPace collar and the Actical monitor simultaneously for 14 days. Output from these devices was compared (correlations), and output from the PetPace device was used to explore differences between groups across the activity and vital sign outputs (including calculated heart rate variability indices).ResultsThere was moderate correlation between the PetPace collar and Actical monitor output (R2 = 0.56, p < 0.001). Using data generated by the PetPace collar, OA-pain dogs had lower overall activity counts and spent less time standing than healthy dogs. Healthy dogs spent more time at higher activity levels than OA-pain dogs. Certain heart rate variability indices in OA-pain dogs were lower than in healthy dogs.Conclusions and clinical relevanceThe results of this study suggest that the PetPace collar can detect differences between healthy dogs and those with OA-pain, and that OA-pain negatively impacts overall activity levels in dogs, and especially higher intensity activity.}, journal={FRONTIERS IN PAIN RESEARCH}, publisher={Frontiers Media SA}, author={Ortiz, Avery Rowlison and Belda, Beatriz and Hash, Jon and Enomoto, Masataka and Robertson, James and Lascelles, B. Duncan X.}, year={2022}, month={Sep} }
 @article{gupta_chiavaccini_minnema_chiu_knazovicky_hash_mishra_lascelles_2021, title={Serum artemin is not correlated with sensitivity within dogs with naturally occurring osteoarthritis pain}, volume={11}, ISSN={["2045-2322"]}, url={https://europepmc.org/articles/PMC7988108}, DOI={10.1038/s41598-021-85976-y}, abstractNote={AbstractOsteoarthritis (OA) pain is associated with peripheral and central sensitization in humans and results in widespread increased sensitivity across the body. Sensitization contributes to the OA-associated pain (OAP) state. We recently identified increased levels of an endogenous neurotrophic factor, artemin (ARTN), in dogs with OAP compared to healthy pain-free controls. Circulating ARTN released from damaged tissues in OA, may play a central role in widespread sensitivity and pain. However, the relationship between ARTN and somatosensory sensitivity remains unknown. The study aimed to assess the relationship between serum ARTN concentrations and measures of sensitivity in dogs with OAP using quantitative sensory testing. We hypothesized that there would be a positive association between circulating ARTN and increased sensitivity to mechanical and thermal stimuli in dogs with OAP. We used linear and logistic regression models to assess the relationship between ARTN, sensitization, and pain within a cohort of 43 dogs with spontaneous OAP. Serum ARTN was not associated with the degree of sensitization within dogs with OAP. Further, across dogs with varying OAP severity, we did not find any association between ARTN, and clinical measures of joint pain and disability. Although a relationship between ARTN and joint pain was not ruled out.}, number={1}, journal={SCIENTIFIC REPORTS}, author={Gupta, Ankita and Chiavaccini, Ludovica and Minnema, Laura M. and Chiu, King Wa and Knazovicky, David and Hash, Jonathan A. and Mishra, Santosh K. and Lascelles, B. Duncan X.}, year={2021}, month={Mar} }
 @article{diamond_scharf_chiu_hash_mathews_2021, title={A randomized, prospective, comparative trial of a variable-angle versus fixed-angle endoscope for exploratory thoracoscopy in canine cadavers}, volume={50}, ISSN={["1532-950X"]}, DOI={10.1111/vsu.13449}, abstractNote={AbstractObjectiveTo compare the efficacy of a variable‐angle endoscope (VAE) for canine thoracoscopic exploration to a traditional fixed‐angle endoscope (FAE).Study designRandomized, prospective, comparative study.Sample populationFive fresh canine cadavers.MethodsTwelve predetermined anatomical locations were labeled after median sternotomy in each cadaveric thorax. Two board‐certified veterinary surgeons performed thoracoscopic evaluation of each thorax using a fixed‐angle (30°) and a variable‐angle (0°‐120°) endoscope with and without lungs mechanically ventilated. The order of surgeon, lung ventilation, and endoscope were determined using a randomized block design. Time to visualize each anatomical location was compared for surgeon, endoscope, and lung ventilation status. Primary outcome measures were time to individual anatomical location, total simulated thoracoscopic exploration time, and ability to identify anatomical location within the designated time period.ResultsLung ventilation (difference = 184 seconds, P = .015, 95% CI = 45‐342 seconds) and endoscope type (difference = 112 seconds, P = .029; 95% CI = 10‐213 seconds) had an effect on the cumulative time for complete thoracoscopic exploration. The VAE shortened the time to identify three of the 12 anatomical locations when controlling for the effects of lung ventilation. Use of the VAE did not improve time to identification for any locations compared to the FAE when lungs were not ventilated. The VAE facilitated significantly shorter cumulative thoracoscopic exploration time compared with the FAE. Failure to identify predetermined locations was more common with the FAE than with the VAE.ConclusionUse of a rigid VAE decreased cumulative thoracoscopic exploration time and provided an alternative to one‐lung ventilation for circumventing the visual impediments of lung ventilation.Clinical significanceThis cadaveric study provides evidence that one‐lung ventilation and use of a VAE may improve surgeon efficiency during exploratory thoracoscopy.}, journal={VETERINARY SURGERY}, author={Diamond, Danielle M. and Scharf, Valery F. and Chiu, King Wa and Hash, Jonathan A. and Mathews, Kyle G.}, year={2021}, month={Jul}, pages={O17–O25} }
 @article{vlaming_mathews_hash_keenihan_sommer_borst_vaden_2022, title={Creation of a Continent Urinary Bladder Reservoir Vascularized by Omentum as a Possible Surgical Option for Canine Trigonal/Urethral Urothelial Carcinoma}, volume={35}, ISSN={["1521-0553"]}, DOI={10.1080/08941939.2020.1864797}, abstractNote={Abstract Surgical procedures that maintain continence with minimal complication following resection of trigono-urethral urothelial carcinoma (UC) are limited in canines; therefore, palliative options are often pursued. A feasible tumor resection option may improve disease control and survival. The study’s objective was to evaluate a continent urine reservoir created from the urinary bladder body and vascularized solely by omentum. We hypothesized that a viable urine reservoir could be created, and staged omentalization would provide improved vascularity. Nine normal female Beagles were randomized to one of three groups. Group A urinary bladders were transected cranial to the ureteral papillae to create a closed bladder vesicle which was concomitantly omentalized. Group B underwent omentalization two weeks prior to vesicle creation. Based on Group A and B results, Group C underwent neoureterocystostomy and omentalization followed by neoreservoir formation and tube cystostomy 2 weeks later. Serial ultrasounds and histopathology confirmed adequate omental neovascularization in Groups B and C with continent Group C neoreservoirs maintained for 2 months. Some pylectasia and ureteral dilation was documented in all Group C dogs at variable timepoints. Progressive hydroureteronephrosis developed in 2/6 kidneys. Transient azotemia was noted in only 1 Group C dog, although all developed treatable urinary tract infections. The sample size is limited, and the efficacy of this technique in providing disease control for UC is unknown. However, this novel option could allow for primary UC resection while providing continence and limiting complications. Postoperative local or systemic adjuvant therapy, ultrasonographic neoreservoir monitoring, and BRAF analysis would be indicated.}, number={3}, journal={JOURNAL OF INVESTIGATIVE SURGERY}, author={Vlaming, Annemarieke and Mathews, Kyle G. and Hash, Jonathan A. and Keenihan, Erin K. and Sommer, Samantha and Borst, Luke and Vaden, Shelly L.}, year={2022}, month={Feb}, pages={481–495} }
 @article{chiu_hash_meyers_lascelles_2020, title={The effect of spontaneous osteoarthritis on conditioned pain modulation in the canine model}, volume={10}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-020-58499-1}, abstractNote={AbstractEndogenous Pain Modulation (EPM) impairment is a significant contributor to chronic pain. Conditioned pain modulation (CPM) testing assesses EPM function. Osteoarthritic (OA) dogs are good translational models, but CPM has not been explored. Our aim was to assess EPM impairment in OA dogs compared to controls using CPM. We hypothesized that CPM testing would demonstrate EPM impairment in OA dogs compared to controls. Dogs with stifle/hip OA and demographically-matched controls were recruited. The pre-conditioning test stimulus, using mechanical/thermal quantitative sensory testing (MQST or TQST), were performed at the metatarsus. A 22N blunt probe (conditioning stimulus) was applied to the contralateral antebrachium for 2 minutes, followed by MQST or TQST (post-conditioning test stimulus). The threshold changes from pre to post-conditioning (∆MQST and ∆TQST) were compared between OA and control dogs. Twenty-four client-owned dogs (OA, n = 11; controls, n = 13) were recruited. The ∆MQST(p < 0.001) and ∆TQST(p < 0.001) increased in control dogs but not OA dogs (∆MQST p = 0.65; ∆TQST p = 0.76). Both ∆MQST(p < 0.001) and ∆TQST(p < 0.001) were different between the OA and control groups. These are the first data showing that EPM impairment is associated with canine OA pain. The spontaneous OA dog model may be used to test drugs that normalize EPM function.}, number={1}, journal={SCIENTIFIC REPORTS}, author={Chiu, King Wa and Hash, Jon and Meyers, Rachel and Lascelles, B. Duncan X.}, year={2020}, month={Feb} }