Jose Manuel Bruno-Barcena

https://orcid.org/0000-0003-2876-1496

Industrial Microbiology, Bioprocessing, Microbiology, Prebiotics, Probiotics

Jose M. Bruno-Barcena, Ph.D. is a Professor of Microbiology in the Department of Plant and Microbial Biology at NC State University. Dr. Bruno-Barcena is a translational industrial microbiologist with an effective research program, which includes strong collaborative relationships within the biotechnology industry, and a passion for bioprocess workforce training. During the last two decades at NCSU he contributed to the teaching, training, and service mission of the University including inter and intra college teaching efforts with interactions at all levels, from undergraduate and graduate students to industry outreach programs including training of professionals and US Food and Drug Administration (FDA) inspectors. His research projects focus on microbial physiology, exploring bioreactor technology and traditional fermentation processes using renewable carbon sources. He currently aims to study the physiology of gut microbial communities using traditional preclinical in vivo models as well as in vitro approaches involving cultivation of infant gut microbial communities using packed-bed reactors. He has published over 35 peer-reviewed publications, 6 book chapters, and 8 invention disclosures.

Works (54)

2022 article

Application of raw industrial sweetpotato hydrolysates for butanol production by Clostridium beijerinckii NCIMB 8052

Zuleta-Correa, A., Chinn, M. S., & Bruno-Barcena, J. M. (2022, August 1). BIOMASS CONVERSION AND BIOREFINERY, Vol. 8.

By: A. Zuleta-Correa n, M. Chinn & J. Bruno-Barcena

author keywords: Ipomoea batatas; ABE fermentation; Consolidated bioprocessing; Anthocyanin; Phenolics; Flour
Sources: ORCID, Web Of Science
Added: August 2, 2022

2022 journal article

Randomized placebo-controlled trial of feline-origin Enterococcus hirae probiotic effects on preventative health and fecal microbiota composition of fostered shelter kittens

FRONTIERS IN VETERINARY SCIENCE, 9.

By: J. Gookin, S. Strong, J. Bruno-Barcena, S. Stauffer, S. Williams, E. Wassack, M. Azcarate-Peril, M. Estrada ...

author keywords: 16S rRNA gene amplicon sequencing; diarrheal pathogens; growth and survival; infection; polymerase chain reaction; shelter medicine
Sources: ORCID, Web Of Science
Added: December 8, 2022

2021 journal article

Safety and Modulatory Effects of Humanized Galacto-Oligosaccharides on the Gut Microbiome

FRONTIERS IN NUTRITION, 8.

By: J. Arnold*, H. Whittington, S. Dagher, J. Roach*, M. Azcarate-Peril* & J. Bruno-Barcena

author keywords: galactooligosaccharide (GOS); N-Acetyl-D-lactosamine (LacNAc); safety; human milk oligosaccharides (HMOs); LacNAc synthesis; mouse models
Sources: ORCID, Web Of Science
Added: April 9, 2021

2021 journal article

The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

MICROBIOME, 9(1).

By: J. Arnold*, J. Roach*, S. Fabela*, E. Moorfield*, S. Ding*, E. Blue*, S. Dagher, S. Magness ...

author keywords: Gut microbiome; Prebiotics; Bifidobacterium; Intestinal permeability; Host-microbiota interactions; Diet; Antibiotics; Metagenomics; Transcriptomics; Organoids
Sources: Web Of Science, ORCID
Added: February 22, 2021

2021 article

The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut (vol 9, 31, 2021)

Arnold, J. W., Roach, J., Fabela, S., Moorfield, E., Ding, S., Blue, E., … Azcarate-Peril, M. A. (2021, February 26). MICROBIOME, Vol. 9.

By: J. Arnold*, J. Roach*, S. Fabela*, E. Moorfield*, S. Ding*, E. Blue*, S. Dagher, S. Magness ...

Sources: Web Of Science, ORCID
Added: March 22, 2021

2020 journal article

Accelerated Biodegradation of the Agrochemical Ametoctradin by Soil-Derived Microbial Consortia

Frontiers in Microbiology.

author keywords: biodegradation; fungicide; ametoctradin; microbial consortium; packed-bed reactor
Source: ORCID
Added: August 26, 2020

2020 journal article

An iterative approach to improve xylose consumption by Clostridium autoethanogenum: From substrate concentration to pH adjustment

Biomass and Bioenergy.

author keywords: Clostridium autoethanogenum; Acetogen; pH optimization; Pentose fermentation; Xylose concentration
Source: ORCID
Added: August 8, 2020

2020 journal article

Draft Genome Sequence of Lactobacillus rhamnosus NCB 441, Isolated from Egyptian White Domiati Cheese

Microbiology Resource Announcements.

By: M. Moore n, H. Whittington n, M. Azcarate-Peril* & J. Bruno-Bárcena n

Ed(s): K. Stedman

Source: ORCID
Added: December 3, 2020

2020 journal article

Two-stage continuous conversion of carbon monoxide to ethylene by whole cells of Azotobacter vinelandii.

Applied and Environmental Microbiology, 86(11).

By: J. Natzke n & J. Bruno-Bárcena n

Ed(s): C. Vieille

author keywords: Azotobacter vinelandii; carbon monoxide; chemostat; ethylene; vanadium nitrogenase
Source: ORCID
Added: April 11, 2020

2020 journal article

Use of unconventional mixed Acetone-Butanol-Ethanol solvents for anthocyanin extraction from Purple-Fleshed sweetpotatoes

Food Chemistry, 314, 125959.

author keywords: Ipomoea batatas; Anthocyanidins; Phenolics; Cyanidin; Peonidin; Temperature; Flour
Sources: ORCID, Crossref
Added: December 11, 2019

2019 book

How Fermented Foods Feed a Healthy Gut Microbiota: A Nutrition Continuum

By: J. Bruno-Barcena

Source: ORCID
Added: December 9, 2019

2019 journal article

Influence of the intestinal microbiota on disease susceptibility in kittens with experimentally-induced carriage of atypical enteropathogenic Escherichia coli

Veterinary Microbiology, 231, 197–206.

By: V. Watson*, M. Jacob n, J. Bruno-Bárcena n, S. Amirsultan*, S. Stauffer*, V. Píqueras*, R. Frias*, J. Gookin

author keywords: aEPEC; Animal model; Feline; Probiotic; Enterococcus hirae
Sources: ORCID, Crossref
Added: April 15, 2019

2019 chapter

Production and Conservation of Starter Cultures: From “Backslopping” to Controlled Fermentations

In A. R. In: Azcarate-Peril M. (Ed.), How Fermented Foods Feed a Healthy Gut Microbiota: A Nutrition Continuum.

By: J. Bruno-Barcena

Ed(s): A. In: Azcarate-Peril M.

author keywords: Backslopping; Food preservation; Food biotransformation; Fermentation; Traditional fermentation; Industrial fermentation; Culture production
Source: ORCID
Added: December 9, 2019

2018 journal article

Azotobacter vinelandii Nitrogenase Activity, Hydrogen Production, and Response to Oxygen Exposure

Applied and Environmental Microbiology.

By: Publons

author keywords: hydrogen; in vivo nitrogenase activity; molybdenum; vanadium; iron
Source: ORCID
Added: May 4, 2020

2018 journal article

Azotobacter vinelandii nitrogenase activity, hydrogen production, and response to oxygen exposure

Applied and Environmental Microbiology, 84(16).

By: J. Natzke, J. Noar & J. Bruno-Bárcena

Source: ORCID
Added: May 4, 2020

2018 journal article

Azotobacter vinelandii: The source of 100 years of discoveries and many more to come

Microbiology (United Kingdom), 164(4), 421–436.

By: J. Noar n & J. Bruno-Bárcena

author keywords: biotechnology; biopolymers; bioenergy; nitrogen; respiration
Source: ORCID
Added: May 4, 2020

2018 journal article

Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus

NUTRIENTS, 10(10).

By: J. Arnold*, J. Simpson*, J. Roach*, J. Bruno-Barcena & M. Azcarate-Peril*

author keywords: prebiotics for lactose intolerance; galacto-oligosaccharides; Lactobacillus; probiotics; beta-galactosidases
Sources: Web Of Science, ORCID
Added: November 19, 2018

2017 journal article

Evidence of mixotrophic carbon-capture by n-butanol-producer Clostridium beijerinckii

Scientific Reports, 7(1).

By: W. Sandoval-Espinola n, M. Chinn, M. Thon* & J. Bruno-Bárcena

Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2017 journal article

Packed-bed Reactors, Dynamic Culturing, and Stress-Directed Microbiome Selection; a Case Study of Agrochemicals Soil Biodegradation

In Vitro Cellular and Developmental Biology - Animal. https://publons.com/publon/31684349/

By: Publons

Source: ORCID
Added: May 4, 2020

2016 journal article

A novel N-terminal region of the membrane beta-hexosyltransferase: its role in secretion of soluble protein by Pichia pastoris

MICROBIOLOGY-SGM, 162(1), 23–34.

By: S. Dagher & J. Bruno-Barcena

Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain

Applied Microbiology and Biotechnology, 101(4), 1615–1630.

By: J. Whitham n, M. Schulte n, B. Bobay*, J. Bruno-Barcena, M. Chinn, M. Flickinger, J. Pawlak, A. Grunden

author keywords: Acetyl-coA; Carbon monoxide dehydrogenase; Clostridium ljungdahlii; Ethanol; Wood-Ljungdahl pathway
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2016 journal article

High purity galacto-oligosaccharides enhance specific Bifidobacterium species and their metabolic activity in the mouse gut microbiome.

Beneficial Microbes, 7(2), 247–264.

By: A. Monteagudo-Mera*, J. Arthur*, C. Jobin*, T. Keku*, J. Bruno-Barcena & M. Azcarate-Peril*

author keywords: mouse gut microbiome; prebiotics; Bifidobacterium
Source: ORCID
Added: March 4, 2019

2016 review

Protons and pleomorphs: aerobic hydrogen production in Azotobacters

[Review of ]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 32(2).

By: J. Noar & J. Bruno-Barcena

author keywords: Hydrogen; Nitrogenase; Azotobacter; Heterotroph; Aerobe
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Aerobic Hydrogen Production via Nitrogenase in Azotobacter vinelandii CA6

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(13), 4507–4516.

By: J. Noar, T. Loveless n, J. Luis Navarro-Herrero*, J. Olson & J. Bruno-Barcena

Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Biology of reactive oxygen species, oxidative stress, and antioxidants in lactic acid bacteria

Probiotics and Prebiotics: Current Research and Future Trends, 205–218.

By: M. Leite, B. Troxell, J. Bruno-Bárcena & H. Hassan

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Galacto-oligosaccharides and colorectal cancer: Feeding our intestinal probiome

JOURNAL OF FUNCTIONAL FOODS, 12, 92–108.

By: J. Bruno-Barcena & M. Azcarate-Peril*

author keywords: Prebiotics; Galacto-oligosaccharides; Colorectal cancer; Microbiome; Probiome
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Inoculum optimization of Clostridium beijerinckii for reproducible growth

FEMS Microbiology Letters, 362(19), fnv164.

By: W. Sandoval-Espinola n, M. Chinn & J. Bruno-Barcena

Ed(s): M. Sauer

author keywords: Clostridium; inoculum; specific growth rate; quality curve; Clostridium beijerinckii; adaptation
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2015 journal article

Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies

Scientific Data, 2(1).

Sources: ORCID, Crossref
Added: March 4, 2019

2014 journal article

Complete Genome Sequence of Solvent-Tolerant Clostridium beijerinckii Strain SA-1

Microbiology Resource Announcements.

By: Publons

Source: ORCID
Added: May 4, 2020

2014 journal article

Complete Genome Sequence of Solvent-Tolerant Clostridium beijerinckii Strain SA-1.

Genome Announcements, 2(6).

By: J. Noar n, S. Makwana n & J. Bruno-Bárcena

Source: ORCID
Added: March 4, 2019

2013 journal article

Comparative phenotypic analysis and genome sequence of Clostridium beijerinckii SA-1, an offspring of NCIMB 8052

MICROBIOLOGY-SGM, 159(PART 12), 2558–2570.

By: W. Sandoval-Espinola n, S. Makwana n, M. Chinn, M. Thon*, M. Andrea Azcarate-Peril* & J. Bruno-Barcena

Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Complete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6.

Genome Announcements, 6(3).

By: J. Noar n & J. Bruno-Bárcena

Source: ORCID
Added: March 4, 2019

2013 journal article

Genome Sequence of the Autotrophic Acetogen Clostridium autoethanogenum JA1-1 Strain DSM 10061, a Producer of Ethanol from Carbon Monoxide

Genome Announcements, 1(4).

By: J. Bruno-Barcena, M. Chinn & A. Grunden n

Sources: ORCID, Crossref
Added: March 4, 2019

2013 journal article

Heterologous Expression of a Bioactive beta-Hexosyltransferase, an Enzyme Producer of Prebiotics, from Sporobolomyces singularis

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(4), 1241–1249.

By: S. Dagher, M. Azcarate-Peril* & J. Bruno-Barcena

Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Meta-Analysis and Functional Validation of Nutritional Requirements of Solventogenic Clostridia Growing under Butanol Stress Conditions and Coutilization of D-Glucose and D-Xylose

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(13), 4473–4485.

By: H. Heluane n, M. Evans, S. Dagher n & J. Bruno-Barcena

Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 review

The intestinal microbiota, gastrointestinal environment and colorectal cancer: a putative role for probiotics in prevention of colorectal cancer?

[Review of ]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 301(3), G401–G424.

By: M. Azcarate-Peril*, M. Sikes & J. Bruno-Barcena

author keywords: lactic acid bacteria
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 article

Cell Immobilization for Production of Lactic Acid: Biofilms Do It Naturally

ADVANCES IN APPLIED MICROBIOLOGY, VOL 71, Vol. 71, pp. 113–148.

By: S. Dagher, A. Ragout*, F. Sineriz* & J. Bruno-Barcena

Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Role of Antioxidant Enzymes in Bacterial Resistance to Organic Acids

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(9), 2747–2753.

By: J. Bruno-Barcena, M. Azcarate-Peril & H. Hassan

Sources: Web Of Science, ORCID
Added: August 6, 2018

2007 journal article

Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 293(4), G729–G738.

By: I. Carroll*, J. Andrus, J. Bruno-Barcena, T. Klaenhammer, H. Hassan & D. Threadgill*

author keywords: probiotic; Lactobacilli; oxidative stress; inflammatory bowel disease
Sources: Web Of Science, ORCID
Added: August 6, 2018

2006 journal article

A closed loop exponential feeding law: Invariance and global stability analysis

JOURNAL OF PROCESS CONTROL, 16(4), 395–402.

By: E. Pico-Marco*, J. Navarro* & J. Bruno-Barcena

author keywords: fed-batch bioreactors; exponential feeding law; partial stability; Monod and Haldane kinetics
Sources: Web Of Science, ORCID
Added: August 6, 2018

2006 journal article

Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(3), 1891–1899.

By: M. Azcarate-Peril n, J. Bruno-Barcena, H. Hassan & T. Klaenhammer

Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

Marker-free chromosomal integration of the manganese superoxide dismutase gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri

FEMS MICROBIOLOGY LETTERS, 246(1), 91–101.

By: J. Bruno-Barcena, M. Azcarate-Peril, T. Klaenhammer & H. Hassan

author keywords: functional gene replacement; manganese superoxide dismutase; oxidative stress; Lactobacillus gasseri; lactic acid bacteria; probiotics
Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

Monitoring stress-related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making.

Applied and Environmental Microbiology, 11(11), 6831–6837.

By: R. Pérez-Torrado*, J. Bruno-Bárcena & E. Matallana*

Source: ORCID
Added: March 4, 2019

2004 journal article

Expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(8), 4702–4710.

By: J. Bruno-Barcena, J. Andrus, S. Libby, T. Klaenhammer & H. Hassan

Sources: Web Of Science, ORCID
Added: August 6, 2018

2002 journal article

pH regulation of enzyme production in Aspergillus nidulans growing in aerobic batch fermenter.

Biotechnology Letters, 4(7), 567–572.

By: Publons

author keywords: Aspergillus nidulans; enzymes production; fungi; regulation of expression
Source: ORCID
Added: March 4, 2019

1999 journal article

Continuous production of L(+)-lactic acid by Lactobacillus casei in two-stage systems.

Applied Microbiology and Biotechnology, 3(3), 316–324.

By: Publons

Source: ORCID
Added: March 4, 2019

1999 journal article

Fluidized bed ion exchange for improving purification of lactic acid from fermentation.

Biotechnology Techniques., 3(3), 201–205.

By: G. Raya-Tonetti*, P. Córdoba*, J. Bruno-Bárcena, F. Siñeriz* & N. Perotti*

author keywords: fluidized bed; ion exchange; lactic acid
Source: ORCID
Added: March 4, 2019

1998 journal article

Chemostat production of plantaricin C by Lactobacillus plantarum LL441

Applied and Environmental Microbiology, 64(9), 3512–3514. http://www.scopus.com/inward/record.url?eid=2-s2.0-3543035172&partnerID=MN8TOARS

Source: ORCID
Added: May 4, 2020

1998 journal article

Chemostat production of plantaricin C by Lactobacillus plantarum LL441

Applied and Environmental Microbiology. https://publons.com/publon/28290537/

By: Publons

Source: ORCID
Added: May 4, 2020

1998 journal article

Chemostat production of plantaricin C by Lactobacillus plantarum LL441.

Applied and Environmental Microbiology, 9. http://europepmc.org/abstract/med/9726907

Source: ORCID
Added: March 4, 2019

1998 journal article

Fragile cells of Lactobacillus casei suitable for plasmid DNA isolation

Biotechnology Techniques. https://publons.com/publon/31684369/

By: Publons

Source: ORCID
Added: May 4, 2020

1998 journal article

Fragile cells of Lactobacillus casei suitable for plasmid DNA isolation.

Biotechnology Techniques., 2(2), 97–99.

By: J. Bruno-Bárcena, M. Azcárate-Peril*, A. Ragout*, G. Font De Valdez*, R. Raya* & F. Siñeriz*

Source: ORCID
Added: March 4, 2019

1998 journal article

Microbial physiology applied to process optimisation: Lactic acid bacteria

Advances in Bioprocess Engineering Volume Ii.

By: Publons

Source: ORCID
Added: May 4, 2020

1997 journal article

Shifts in pH affect the maltose/glycerol co-fermentation by Lactobacillus reuteri

Biotechnology Letters, 19(7), 645–649.

By: G. De Valdez*, A. Ragout*, J. Bruno-Bárcena, H. Diekmann & F. Siñeriz*

Source: ORCID
Added: May 4, 2020

Employment

2020 - present

North Carolina State University Raleigh, NC, US
Professor Plant and Microbial Biology

2014 - 2020

North Carolina State University Raleigh, NC, US
Associated Professor Plant and Microbial Biology

2007 - 2014

North Carolina State University Raleigh, NC, US
Assistant Profesor Microbiology

Education

1994 - 1997

Universidad Nacional de Tucumán San Miguel de Tucuman, AR
PhD Facultad de Ciencias Naturales e Instituto Miguel Lillo

1985 - 1991

Universidad de Oviedo Oviedo, Asturias, ES
Functional Biology