Jose Manuel Bruno-Barcena
Industrial Microbiology, Bioprocessing, Microbiology, Prebiotics, Probiotics
Jose M. Bruno-Barcena, Ph.D. is a Professor of Microbiology in the Department of Plant and Microbial Biology at NC State University. Dr. Bruno-Barcena is a translational industrial microbiologist with an effective research program, which includes strong collaborative relationships within the biotechnology industry, and a passion for bioprocess workforce training. During the last two decades at NCSU he contributed to the teaching, training, and service mission of the University including inter and intra college teaching efforts with interactions at all levels, from undergraduate and graduate students to industry outreach programs including training of professionals and US Food and Drug Administration (FDA) inspectors. His research projects focus on microbial physiology, exploring bioreactor technology and traditional fermentation processes using renewable carbon sources. He currently aims to study the physiology of gut microbial communities using traditional preclinical in vivo models as well as in vitro approaches involving cultivation of infant gut microbial communities using packed-bed reactors. He has published over 35 peer-reviewed publications, 6 book chapters, and 8 invention disclosures.
Works (56)
Updated: December 19th, 2024 05:03
2025 journal article
Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers
Gut Microbes.
Source: ORCID
Added: December 19, 2024
2023 journal article
Structural analysis and functional evaluation of the disordered ß-hexosyltransferase region from <i>Hamamotoa (Sporobolomyces) singularis</i>
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 11.
author keywords: disorder; expression; kinetics; mutagenesis; transglycosylation; Hamamotoa singularis
TL;DR:
Functional analysis of each glycosylation site found within the C-terminal domain revealed reduced total secreted protein activity, indicating that glycosylation at all four locations is required by the host for the secretion of active enzyme and independent of the removed disordered N-terminal region.
(via Semantic Scholar)
open access via www.frontiersin.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: December 31, 2023
2022 article
Application of raw industrial sweetpotato hydrolysates for butanol production by Clostridium beijerinckii NCIMB 8052
Zuleta-Correa, A., Chinn, M. S., & Bruno-Barcena, J. M. (2022, August 1). BIOMASS CONVERSION AND BIOREFINERY, Vol. 8.
author keywords: Ipomoea batatas; ABE fermentation; Consolidated bioprocessing; Anthocyanin; Phenolics; Flour
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 2, 2022
2022 journal article
Randomized placebo-controlled trial of feline-origin Enterococcus hirae probiotic effects on preventative health and fecal microbiota composition of fostered shelter kittens
FRONTIERS IN VETERINARY SCIENCE, 9.
author keywords: 16S rRNA gene amplicon sequencing; diarrheal pathogens; growth and survival; infection; polymerase chain reaction; shelter medicine
TL;DR:
A rationale for use of E. hirae to foster kittens supports a rationale for disease prevention in this young population at high risk for intestinal disease though additional studies are warranted.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: December 8, 2022
2021 journal article
Safety and Modulatory Effects of Humanized Galacto-Oligosaccharides on the Gut Microbiome
FRONTIERS IN NUTRITION, 8.
author keywords: galactooligosaccharide (GOS); N-Acetyl-D-lactosamine (LacNAc); safety; human milk oligosaccharides (HMOs); LacNAc synthesis; mouse models
TL;DR:
The study demonstrated the biological synthesis of hGOS, and signaled its safety and ability to modulate the gut microbiome in vivo, promoting the growth of beneficial microorganisms, including Bifidobacterium and Akkermansia.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 9, 2021
2021 journal article
The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut
MICROBIOME, 9(1).
author keywords: Gut microbiome; Prebiotics; Bifidobacterium; Intestinal permeability; Host-microbiota interactions; Diet; Antibiotics; Metagenomics; Transcriptomics; Organoids
MeSH headings : Aging / drug effects; Aging / genetics; Aging / physiology; Animals; Female; Gastrointestinal Microbiome / drug effects; Intestines / drug effects; Intestines / microbiology; Intestines / physiology; Mice; Mice, Inbred C57BL; Oligosaccharides / pharmacology; Phosphatidylinositol 3-Kinases / metabolism; Prebiotics; Proto-Oncogene Proteins c-akt / metabolism
TL;DR:
Stools from mice exhibiting variable bifidogenic response to GOS injected into colon organoids in the presence of prebiotics reproduced the response and non-response phenotypes observed in vivo suggesting that the composition and functionality of the microbiota are the main contributors to the phenotype.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: February 22, 2021
2021 article
The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut (vol 9, 31, 2021)
Arnold, J. W., Roach, J., Fabela, S., Moorfield, E., Ding, S., Blue, E., … Azcarate-Peril, M. A. (2021, February 26). MICROBIOME, Vol. 9.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 22, 2021
2020 journal article
Accelerated Biodegradation of the Agrochemical Ametoctradin by Soil-Derived Microbial Consortia
Frontiers in Microbiology.
Contributors: H. Whittington n, M. Singh*, C. Ta*, M. Azcarate-Peril * & n
author keywords: biodegradation; fungicide; ametoctradin; microbial consortium; packed-bed reactor
TL;DR:
It was found that biodegradation of ametoctradin in both ex vivo soils and in vitro in the bioreactor correlated with an increase in the relative abundance of Burkholderiales, well characterized microbial degraders of xenobiotic compounds.
(via Semantic Scholar)
Source: ORCID
Added: August 26, 2020
2020 journal article
An iterative approach to improve xylose consumption by Clostridium autoethanogenum: From substrate concentration to pH adjustment
Biomass and Bioenergy.
author keywords: Clostridium autoethanogenum; Acetogen; pH optimization; Pentose fermentation; Xylose concentration
TL;DR:
It is demonstrated that C. autoethanogenum can effectively biotransform xylose at concentrations typically present in lignocellulosic hydrolysate streams.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Source: ORCID
Added: August 8, 2020
2020 journal article
Draft Genome Sequence of Lactobacillus rhamnosus NCB 441, Isolated from Egyptian White Domiati Cheese
Microbiology Resource Announcements.
Ed(s): K. Stedman
TL;DR:
The draft genome sequence of Lactobacillus rhamnosus NCB 441, which was isolated from pickled white cheese samples gathered at Farafra Oasis in New Valley Governorate, Egypt, is reported, with a G+C content of 46.7%.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Source: ORCID
Added: December 3, 2020
2020 journal article
Two-stage continuous conversion of carbon monoxide to ethylene by whole cells of Azotobacter vinelandii.
Applied and Environmental Microbiology, 86(11).
Ed(s): C. Vieille
author keywords: Azotobacter vinelandii; carbon monoxide; chemostat; ethylene; vanadium nitrogenase
MeSH headings : Azotobacter vinelandii / metabolism; Biofuels; Bioreactors; Carbon Monoxide / metabolism; Ethylenes / metabolism
TL;DR:
This work describes a continuous biological method for investigating the nitrogenase-mediated carbon monoxide reductive coupling involved in ethylene production using whole cells of Azotobacter vinelandii and demonstrates the potential to significantly reduce the total energy input required and the ethylene recovery costs, as well as decreasing greenhouse gas emissions associated with current production strategies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science; OpenAlex)
Source: ORCID
Added: April 11, 2020
2019 book
How Fermented Foods Feed a Healthy Gut Microbiota: A Nutrition Continuum
TL;DR:
Select bacteria of the maternal digestive microbiota may access the mammary glands through oraland entero-mammary pathways by involving mononuclear cells for their transport, which would provide new opportunities for manipulating maternal-fetal microbiota, reducing the risk of preterm birth or infant diseases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: December 9, 2019
2019 journal article
Influence of the intestinal microbiota on disease susceptibility in kittens with experimentally-induced carriage of atypical enteropathogenic Escherichia coli
Veterinary Microbiology, 231, 197–206.
Contributors: V. Watson, M. Jacob n, n , S. Amirsultan, S. Stauffer, V. Píqueras *, R. Frias *, J. Gookin n
author keywords: aEPEC; Animal model; Feline; Probiotic; Enterococcus hirae
MeSH headings : Age Factors; Animals; Anti-Infective Agents / pharmacology; Asymptomatic Infections; Bacterial Shedding; Cats / microbiology; Diarrhea / microbiology; Disease Models, Animal; Disease Susceptibility / etiology; Disease Susceptibility / microbiology; Enteropathogenic Escherichia coli; Escherichia coli Infections / veterinary; Feces / microbiology; Gastrointestinal Microbiome; Polymerase Chain Reaction; Probiotics
TL;DR:
Enrichment of the intestinal microbiota with a commensal member of the feline mucosa-associated microbiota, Enterococcus hirae, ameliorated the effects of aEPEC experimental infection on intestinal function and water loss and begin to unravel the mechanisms by which aEPec infection may be able to exploit susceptible hosts.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Crossref, NC State University Libraries
Added: April 15, 2019
2019 chapter
Production and Conservation of Starter Cultures: From “Backslopping” to Controlled Fermentations
In A. R. In: Azcarate-Peril M. (Ed.), How Fermented Foods Feed a Healthy Gut Microbiota: A Nutrition Continuum.
Ed(s): A. In: Azcarate-Peril M.
author keywords: Backslopping; Food preservation; Food biotransformation; Fermentation; Traditional fermentation; Industrial fermentation; Culture production
TL;DR:
New methods of mixed-strain starter culture production, particularly immobilized cell reactors, present attractive alternatives to the more traditional batch reactors due to their ability to produce a more robust and diverse starter all in one step.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: December 9, 2019
2019 journal article
Use of unconventional mixed Acetone-Butanol-Ethanol solvents for anthocyanin extraction from Purple-Fleshed sweetpotatoes
Food Chemistry, 314, 125959.
Contributors: A. Zuleta-Correa n, M. Chinn n , M. Alfaro-Córdoba *, V. Truong n, G. Yencho n & n
author keywords: Ipomoea batatas; Anthocyanidins; Phenolics; Cyanidin; Peonidin; Temperature; Flour
MeSH headings : Acetone / chemistry; Anthocyanins / analysis; Anthocyanins / chemistry; Anthocyanins / isolation & purification; Butanols / chemistry; Chemical Fractionation / methods; Ethanol / chemistry; Ipomoea batatas / chemistry; Phenols / isolation & purification; Plant Extracts / chemistry; Solvents / chemistry
TL;DR:
Results indicated that unconventional diluted solvent mixes containing acetone, butanol, and ethanol were superior or equally efficient for extracting anthocyanins when compared to commonly used concentrated extractants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Crossref, NC State University Libraries
Added: December 11, 2019
2018 journal article
Azotobacter vinelandii Nitrogenase Activity, Hydrogen Production, and Response to Oxygen Exposure
Applied and Environmental Microbiology.
Contributors: J. Natzke n, J. Noar n & n
author keywords: hydrogen; in vivo nitrogenase activity; molybdenum; vanadium; iron
MeSH headings : Azotobacter vinelandii / enzymology; Azotobacter vinelandii / genetics; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Hydrogen / metabolism; Hydroxybutyrates / metabolism; Iron / metabolism; Molybdenum / metabolism; Nitrogen / metabolism; Nitrogen Fixation; Nitrogenase / genetics; Nitrogenase / metabolism; Oxidation-Reduction; Oxygen / metabolism; Polyesters / metabolism; Vanadium / metabolism
TL;DR:
The data presented here show that increasing oxygen exposure limits equally the in vivo activities of all nitrogenase isoenzymes, while under comparative conditions, the Mo nitrogenase enzyme evolves more hydrogen per unit of biomass than the alternative isoenZymes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
7. Affordable and Clean Energy
(Web of Science)
Source: ORCID
Added: May 4, 2020
2018 journal article
Azotobacter vinelandii nitrogenase activity, hydrogen production, and response to oxygen exposure
Applied and Environmental Microbiology, 84(16).
Source: ORCID
Added: May 4, 2020
2018 journal article
Azotobacter vinelandii: The source of 100 years of discoveries and many more to come
Microbiology (United Kingdom), 164(4), 421–436.
Contributors: J. Noar n & n
author keywords: biotechnology; biopolymers; bioenergy; nitrogen; respiration
MeSH headings : Azotobacter vinelandii / enzymology; Azotobacter vinelandii / metabolism; Biofuels; Biopolymers; Hydrogen / metabolism; Metabolic Engineering; Metabolic Networks and Pathways; Nitrogen / metabolism; Nitrogenase / metabolism; Oxidoreductases / metabolism; Oxygen / metabolism
TL;DR:
The history and potential for future research using this versatile microbe are summarized, which includes its ability to produce multiple valuable polymers - bioplastic and alginate in particular; its nitrogen-fixing prowess; its production of potentially useful enzymes and metabolic pathways; and even its biofuel production abilities.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
7. Affordable and Clean Energy
(Web of Science)
Source: ORCID
Added: May 4, 2020
2018 journal article
Prebiotics for Lactose Intolerance: Variability in Galacto-Oligosaccharide Utilization by Intestinal Lactobacillus rhamnosus
NUTRIENTS, 10(10).
Contributors: J. Arnold *, J. Simpson *, J. Roach *, n & M. Azcarate-Peril *
author keywords: prebiotics for lactose intolerance; galacto-oligosaccharides; Lactobacillus; probiotics; beta-galactosidases
MeSH headings : Gastrointestinal Microbiome; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Humans; Lacticaseibacillus rhamnosus / genetics; Lacticaseibacillus rhamnosus / metabolism; Lactose Intolerance / microbiology; Oligosaccharides / metabolism; Prebiotics
TL;DR:
The findings highlight the importance of strain-specific bacterial metabolism in the selection of probiotics and synbiotics to alleviate symptoms of gastrointestinal disorders including lactose intolerance and highlight the need to understand the mechanisms by which human-derived Lactobacillus rhamnosus strains metabolize GOS.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 19, 2018
2017 journal article
Evidence of mixotrophic carbon-capture by n-butanol-producer Clostridium beijerinckii
Scientific Reports, 7(1).
Contributors: W. Sandoval-Espinola n, M. Chinn n , M. Thon * & n
MeSH headings : 1-Butanol / metabolism; Carbon / metabolism; Carbon Dioxide / metabolism; Carbon Isotopes; Clostridium beijerinckii / genetics; Clostridium beijerinckii / growth & development; Clostridium beijerinckii / metabolism; Electrons; Energy Metabolism; Fermentation; Gene Expression Profiling; Genes, Bacterial; Hydrogen / metabolism; Kinetics; Magnetic Resonance Spectroscopy; Models, Biological; Nitrites / metabolism; Time Factors; Transcription, Genetic; Transcriptome / genetics
TL;DR:
Genome- and transcriptome-wide analysis revealed transcription of key C-1 capture and additional energy conservation genes, including partial Wood-Ljungdahl and complete reversed pyruvate ferredoxin oxidoreductase / pyruVate-formate-lyase-dependent pathways.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2017 journal article
Packed-bed Reactors, Dynamic Culturing, and Stress-Directed Microbiome Selection; a Case Study of Agrochemicals Soil Biodegradation
In Vitro Cellular and Developmental Biology - Animal. https://publons.com/publon/31684349/
Source: ORCID
Added: May 4, 2020
2016 journal article
Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain
Applied Microbiology and Biotechnology, 101(4), 1615–1630.
Contributors: J. Whitham n, M. Schulte n, B. Bobay *, n , M. Chinn n , M. Flickinger n , J. Pawlak n , A. Grunden n
author keywords: Acetyl-coA; Carbon monoxide dehydrogenase; Clostridium ljungdahlii; Ethanol; Wood-Ljungdahl pathway
MeSH headings : Acetyl Coenzyme A / metabolism; Aldehyde Oxidoreductases / metabolism; Carbon Dioxide / metabolism; Carbon Monoxide / metabolism; Clostridium / metabolism; Ethanol / metabolism; Multienzyme Complexes / metabolism
TL;DR:
A non-autotrophic hyper ethanol-producing strain of C. ljungdahlii was identified that has utility for further physiology and strain performance studies and as a biocatalyst for industrial applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2016 journal article
High purity galacto-oligosaccharides enhance specific Bifidobacterium species and their metabolic activity in the mouse gut microbiome.
Beneficial Microbes, 7(2), 247–264.
Contributors: A. Monteagudo-Mera *, J. Arthur *, C. Jobin *, T. Keku *, * & M. Azcarate-Peril *
author keywords: mouse gut microbiome; prebiotics; Bifidobacterium
MeSH headings : Animals; Bacteria / classification; Bacteria / genetics; Bacteria / isolation & purification; Bifidobacterium / genetics; Bifidobacterium / isolation & purification; Bifidobacterium / physiology; Female; Fermentation; Galactose / metabolism; Gastrointestinal Microbiome; Gastrointestinal Tract / metabolism; Gastrointestinal Tract / microbiology; Interleukin-12 / genetics; Interleukin-12 / metabolism; Interleukin-6 / genetics; Interleukin-6 / metabolism; Male; Mice; Mice, 129 Strain; Oligosaccharides / administration & dosage; Oligosaccharides / analysis; Oligosaccharides / metabolism; Prebiotics / administration & dosage; Prebiotics / analysis
TL;DR:
The results demonstrate that GOS90 is capable of modulating the intestinal microbiome resulting in expansion of the probiome and decreased butyrate, acetate and lactate concentrations in stools of prebiotic fed mice suggested an increase in colonic absorption and reduced excretion.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: March 4, 2019
2016 review
Protons and pleomorphs: aerobic hydrogen production in Azotobacters
[Review of ]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 32(2).
Contributors: J. Noar n & n
author keywords: Hydrogen; Nitrogenase; Azotobacter; Heterotroph; Aerobe
MeSH headings : Azotobacter / chemistry; Azotobacter / genetics; Azotobacter / metabolism; Heterotrophic Processes; Hydrogen / chemistry; Hydrogen / metabolism; Oxidation-Reduction; Protons; Soil Microbiology
TL;DR:
Questions about the role of H2 in the energy metabolism of nitrogen-fixing Azotobacter, and what yields can be expected from hydrogen-evolving strains, are addressed in the following mini-review.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2015 journal article
A novel N-terminal region of the membrane beta-hexosyltransferase: its role in secretion of soluble protein by Pichia pastoris
MICROBIOLOGY-SGM, 162(1), 23–34.
Contributors: S. Dagher n & n
MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Cell Membrane / chemistry; Cell Membrane / enzymology; Cell Membrane / genetics; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression; Hexosyltransferases / chemistry; Hexosyltransferases / genetics; Hexosyltransferases / metabolism; Kinetics; Molecular Sequence Data; Pichia / genetics; Pichia / metabolism; Protein Transport; Yeasts / chemistry; Yeasts / enzymology; Yeasts / genetics
TL;DR:
Lead interference was demonstrated when MFα preceded the putative classical rBHT(1-22) leader, explaining the limited secretion of soluble protein by P. pastoris strains carrying combinations of the predicted structural domains.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2015 journal article
Aerobic Hydrogen Production via Nitrogenase in Azotobacter vinelandii CA6
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(13), 4507–4516.
Contributors: J. Noar n, T. Loveless*, J. Navarro-Herrero, J. Olson n & n
MeSH headings : Aerobiosis; Azotobacter vinelandii / enzymology; Azotobacter vinelandii / genetics; Azotobacter vinelandii / metabolism; Genome, Bacterial; Hydrogen / metabolism; Iron / metabolism; Metabolic Networks and Pathways / genetics; Nitrogenase / metabolism; Tungsten Compounds / metabolism
TL;DR:
A large deletion in CA6's genome is revealed, encompassing genes related to molybdate and iron transport and hydrogen reoxidation, which may hold promise for developing a novel strategy for production of hydrogen as an energy compound.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2015 journal article
Biology of reactive oxygen species, oxidative stress, and antioxidants in lactic acid bacteria
Probiotics and Prebiotics: Current Research and Future Trends, 205–218.
Contributors: M. Leite, B. Troxell, * & H. Hassan*
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: August 6, 2018
2015 journal article
Inoculum optimization of Clostridium beijerinckii for reproducible growth
FEMS Microbiology Letters, 362(19), fnv164.
Contributors: W. Sandoval-Espinola n, M. Chinn n & n
Ed(s): M. Sauer
author keywords: Clostridium; inoculum; specific growth rate; quality curve; Clostridium beijerinckii; adaptation
MeSH headings : Butanols; Clostridium beijerinckii / growth & development; Culture Media; Ethanol; Fermentation; Industrial Microbiology / methods; Kinetics; Reproducibility of Results
TL;DR:
Evaluating the influence of inoculum age (harvest time) on the subsequent population's maximum specific growth rate, as a signal of population homogeneity, allowed us to understand the transient nature of seed cultures of C. beijerinckii NCIMB 8052 and SA-1, while enabling reproducibility and consistent culture performance.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2015 journal article
Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies
Scientific Data, 2(1).
Contributors: S. Utturkar *, D. Klingeman *, n , M. Chinn n , A. Grunden n , M. Köpke *, S. Brown *
MeSH headings : Algorithms; Clostridium / genetics; Genome, Bacterial; Genomics; Sequence Analysis, DNA / methods
TL;DR:
High quality sequence datasets which span three generations of sequencing technologies, containing six types of data from four NGS platforms and originating from a single microorganism, Clostridium autoethanogenum are described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Sources: ORCID, Crossref, NC State University Libraries
Added: March 4, 2019
2014 journal article
Complete Genome Sequence of Solvent-Tolerant Clostridium beijerinckii Strain SA-1
Microbiology Resource Announcements.
Source: ORCID
Added: May 4, 2020
2014 journal article
Complete Genome Sequence of Solvent-Tolerant Clostridium beijerinckii Strain SA-1.
Genome Announcements, 2(6).
Contributors: J. Noar n, S. Makwana n & n
TL;DR:
This sequence allows for accurate placement of SA-1 as C. beijerinckii, permits functional analyses of mutant phenotypes, and suggests methods for distinguishingSA-1 from its parent.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Source: ORCID
Added: March 4, 2019
2014 journal article
Galacto-oligosaccharides and colorectal cancer: Feeding our intestinal probiome
JOURNAL OF FUNCTIONAL FOODS, 12, 92–108.
Contributors: n & M. Azcarate-Peril *
author keywords: Prebiotics; Galacto-oligosaccharides; Colorectal cancer; Microbiome; Probiome
TL;DR:
This review summarizes research on prebiotics bioassimilation, specifically β (1-4) GOS, and their potential role in CRC, and evaluates research that show that the impact of prebiotic on host physiology can be direct or through modulation of the gut intestinal microbiome.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2013 journal article
Comparative phenotypic analysis and genome sequence of Clostridium beijerinckii SA-1, an offspring of NCIMB 8052
MICROBIOLOGY-SGM, 159(PART 12), 2558–2570.
Contributors: W. Sandoval-Espinola n, S. Makwana n, M. Chinn n , M. Thon *, M. Andrea Azcárate-Peril * & n
MeSH headings : Butanols / metabolism; Butanols / toxicity; Carbon / metabolism; Clostridium beijerinckii / drug effects; Clostridium beijerinckii / genetics; Clostridium beijerinckii / growth & development; Clostridium beijerinckii / metabolism; Culture Media / chemistry; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Fructose / metabolism; Genome, Bacterial; Glucose / metabolism; Growth Inhibitors / toxicity; Metabolic Networks and Pathways / genetics; Molecular Sequence Data; Polymorphism, Genetic; Sequence Analysis, DNA; Solvents / metabolism; Solvents / toxicity
TL;DR:
Assessment of SA-1 solventogenic capabilities when growing on a previously validated medium containing, as carbon- and energy-limiting substrates, sucrose and the products of its hydrolysis d-glucose and d-fructose showed that it is a solvent hyper-producing strain capable of generating up to 16.1 g l(-1) of butanol and 26.3 g l −1 of total solvents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2013 journal article
Complete Genome Sequences of Azotobacter vinelandii Wild-Type Strain CA and Tungsten-Tolerant Mutant Strain CA6.
Genome Announcements, 6(3).
Contributors: J. Noar n & n
TL;DR:
The complete genome sequences of Azotobacter vinelandii mutant strain CA6 and its parent wild-type strain, CA, are reported to provide a genetic basis for these mutant phenotypes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Source: ORCID
Added: March 4, 2019
2013 journal article
Genome Sequence of the Autotrophic Acetogen Clostridium autoethanogenum JA1-1 Strain DSM 10061, a Producer of Ethanol from Carbon Monoxide
Genome Announcements, 1(4).
Contributors: n , M. Chinn n & A. Grunden n
TL;DR:
Clostridium autoethanogenum is an anaerobic, autotrophic acetogen that is capable of converting CO and CO2 into ethanol and acetate and the findings obtained from annotation of the genome sequence are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: ORCID, Crossref, NC State University Libraries
Added: March 4, 2019
2012 journal article
Heterologous Expression of a Bioactive beta-Hexosyltransferase, an Enzyme Producer of Prebiotics, from Sporobolomyces singularis
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(4), 1241–1249.
Contributors: S. Dagher n , M. Azcarate-Peril * & n
MeSH headings : Basidiomycota / enzymology; Basidiomycota / genetics; Cloning, Molecular; Enzyme Inhibitors / metabolism; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Galactosyltransferases / chemistry; Galactosyltransferases / genetics; Galactosyltransferases / metabolism; Gene Expression; Glucose / metabolism; Hydrogen-Ion Concentration; Kinetics; Lactose / metabolism; Pichia / enzymology; Pichia / genetics; Prebiotics; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Temperature
TL;DR:
The heterologous expression of an optimized synthetically produced version of the β-hexosyltransferase gene (Bht) from Sporobolomyces singularis is described, the first report of a bioactive BHT from S. singularis that has been heterologously expressed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2011 journal article
Meta-Analysis and Functional Validation of Nutritional Requirements of Solventogenic Clostridia Growing under Butanol Stress Conditions and Coutilization of D-Glucose and D-Xylose
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(13), 4473–4485.
Contributors: H. Heluane n, M. Dagher n & n
MeSH headings : Biofuels; Biotechnology / methods; Butanols / toxicity; Clostridium beijerinckii / drug effects; Clostridium beijerinckii / growth & development; Clostridium beijerinckii / metabolism; Culture Media / chemistry; Fermentation; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Glucose / metabolism; Stress, Physiological; Xylose / metabolism
TL;DR:
Results indicated that C. beijerinckii SA-1 was able to coferment hexose/pentose sugar mixtures in the absence of a glucose repression effect, and the analysis suggests that the solvent and acid resistance mechanisms found in this strain are differentially regulated compared to strain NRRL B-527.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2011 review
The intestinal microbiota, gastrointestinal environment and colorectal cancer: a putative role for probiotics in prevention of colorectal cancer?
[Review of ]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 301(3), G401–G424.
Contributors: M. Azcárate-Peril *, M. Sikes n & n
author keywords: lactic acid bacteria
MeSH headings : Antimutagenic Agents / pharmacology; Bile / physiology; Carcinogens; Cell Adhesion; Colorectal Neoplasms / genetics; Colorectal Neoplasms / microbiology; Colorectal Neoplasms / physiopathology; Colorectal Neoplasms / prevention & control; Diet; Gastrointestinal Tract / microbiology; Gastrointestinal Tract / physiology; Humans; Intestinal Mucosa / physiology; Intestines / microbiology; Metagenome; Oxidative Stress; Probiotics / therapeutic use
TL;DR:
Examination of probiotic metabolic activities that may have an effect on the prevention of CRC by scavenging toxic compounds or preventing their generation in situ are examined.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2010 article
Cell Immobilization for Production of Lactic Acid: Biofilms Do It Naturally
ADVANCES IN APPLIED MICROBIOLOGY, VOL 71, Vol. 71, pp. 113–148.
Contributors: S. Dagher n , A. Ragout*, F. Siñeriz * & n
MeSH headings : Biofilms / growth & development; Bioreactors; Biotechnology / methods; Cells, Immobilized; Fermentation; Industrial Microbiology / methods; Lactic Acid / biosynthesis; Lactobacillus / classification; Lactobacillus / growth & development; Lactobacillus / metabolism; Lactobacillus / physiology
TL;DR:
This chapter presents the results of a search for optimum parameters of lactic acid producing microorganisms and how they are affected by the physical, chemical, and biological variables of the process.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2010 journal article
Role of Antioxidant Enzymes in Bacterial Resistance to Organic Acids
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(9), 2747–2753.
Contributors: n , M. Andrea Azcárate-Peril * & H. Hassan*
MeSH headings : Antioxidants / metabolism; Antioxidants / physiology; Bacterial Proteins / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; Hydrogen-Ion Concentration; Iron / metabolism; Lactic Acid / toxicity; Oxidative Stress; Streptococcus thermophilus / drug effects; Streptococcus thermophilus / enzymology; Stress, Physiological; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism; Superoxide Dismutase / physiology
TL;DR:
It is suggested that acid stress triggers an iron-mediated oxidative stress that can be ameliorated by MnSOD and iron chelators and may explain the ability of some microorganisms to survive better in acidified environments, as in acid foods during fermentation and accumulation of lactic acid or during passage through the low pH of the stomach.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2007 journal article
Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 293(4), G729–G738.
Contributors: I. Carroll *, J. Andrus*, n , T. Klaenhammer n, H. Hassan n & D. Threadgill *
author keywords: probiotic; Lactobacilli; oxidative stress; inflammatory bowel disease
MeSH headings : Animals; Anti-Inflammatory Agents / therapeutic use; Colitis / drug therapy; Colitis / pathology; Colon / pathology; Cyclooxygenase 2 / metabolism; Disease Models, Animal; Immunohistochemistry; Interleukin-10 / deficiency; Lactobacillus / enzymology; Mice; Peroxidase / metabolism; Probiotics / therapeutic use; Specific Pathogen-Free Organisms; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / therapeutic use
TL;DR:
This study demonstrates that L. gasseri producing MnSOD has significant anti-inflammatory activity that reduces the severity of colitis in the IL-10-deficient mouse.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2006 journal article
Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(3), 1891–1899.
Contributors: M. Azcarate-Peril n, * , H. Hassan* & T. Klaenhammer n
MeSH headings : Carboxy-Lyases / genetics; Carboxy-Lyases / metabolism; Coenzyme A-Transferases / genetics; Coenzyme A-Transferases / metabolism; Gene Expression Regulation, Bacterial; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Oligonucleotide Array Sequence Analysis; Operon; Oxalates / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic
TL;DR:
Transcriptional analysis using cDNA microarrays and reverse transcription-quantitative PCR revealed that mildly acidic conditions were a prerequisite for frc and oxc transcription, andOxalate-dependent induction of these genes occurred only in cells first adapted to subinhibitory concentrations of oxalate and then exposed to pH 5.5.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2005 journal article
A closed loop exponential feeding law: Invariance and global stability analysis
JOURNAL OF PROCESS CONTROL, 16(4), 395–402.
Contributors: E. Picó-Marco *, J. Navarro * & n
author keywords: fed-batch bioreactors; exponential feeding law; partial stability; Monod and Haldane kinetics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2005 journal article
Marker-free chromosomal integration of the manganese superoxide dismutase gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri
FEMS MICROBIOLOGY LETTERS, 246(1), 91–101.
Contributors: n , M. Azcárate-Peril n, T. Klaenhammer n & H. Hassan n
author keywords: functional gene replacement; manganese superoxide dismutase; oxidative stress; Lactobacillus gasseri; lactic acid bacteria; probiotics
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / analysis; Chromosomes, Bacterial / genetics; Gene Expression; Hydrogen Peroxide / pharmacology; Lactobacillus / enzymology; Lactobacillus / genetics; Lactobacillus / growth & development; Plasmids / genetics; RNA, Bacterial / analysis; RNA, Messenger / analysis; Streptococcus thermophilus / enzymology; Streptococcus thermophilus / genetics; Superoxide Dismutase / genetics; Transformation, Bacterial
TL;DR:
This is the first report showing the successful utilization of the pORI plasmids system to generate marker-free gene integration in L. gasseri strains.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2005 journal article
Monitoring stress-related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making.
Applied and Environmental Microbiology, 11(11), 6831–6837.
Contributors: R. Pérez-Torrado *, * & E. Matallana *
MeSH headings : Biomass; Culture Media; Fermentation; Gene Expression Regulation, Fungal; Heat-Shock Response; Osmotic Pressure; Oxidative Stress; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / growth & development; Saccharomyces cerevisiae / physiology; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism; Wine / microbiology
TL;DR:
It is suggested that the cells were affected by osmotic and oxidative stresses, demonstrating that these are the major causes of the stress response throughout the process of wine yeast biomass production.
(via Semantic Scholar)
Source: ORCID
Added: March 4, 2019
2004 journal article
Expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(8), 4702–4710.
Contributors: * , J. Andrus*, S. Libby*, T. Klaenhammer n & H. Hassan n
MeSH headings : Cloning, Molecular; Humans; Hydrogen Peroxide / pharmacology; Intestines / microbiology; Lactobacillus / classification; Lactobacillus / drug effects; Lactobacillus / enzymology; Lactobacillus / genetics; Oxidative Stress / drug effects; Protein Biosynthesis; Streptococcus / enzymology; Streptococcus / genetics; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism; Transcription, Genetic
TL;DR:
The data show that MnSOD protects cells against hydrogen peroxide by removing O2·− and preventing the redox cycling of iron and is the first report of a sodA from S. thermophilus being expressed in other lactic acid bacteria.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2002 journal article
pH regulation of enzyme production in Aspergillus nidulans growing in aerobic batch fermenter.
Biotechnology Letters, 4(7), 567–572.
Contributors: * , M. Lucca *, F. Siñeriz * & D. Ramón *
author keywords: Aspergillus nidulans; enzymes production; fungi; regulation of expression
TL;DR:
Changing the pH from 5.5 to 4.5 or 7 after induction resulted in differential expression of the homologous and heterologous proteins, indicating versatile industrial strains capable of differentially producing mixtures of extracellular enzymes in response to ambient pH can be produced.
(via Semantic Scholar)
Source: ORCID
Added: March 4, 2019
1999 journal article
Continuous production of L(+)-lactic acid by Lactobacillus casei in two-stage systems.
Applied Microbiology and Biotechnology, 3(3), 316–324.
Contributors: * , A. Ragout*, P. Córdoba* & F. Siñeriz *
MeSH headings : Biomass; Bioreactors / microbiology; Cells, Immobilized / metabolism; Culture Media / chemistry; Fermentation; Glucose / metabolism; Hot Temperature; Hydrogen-Ion Concentration; Industrial Microbiology / methods; Lactic Acid / biosynthesis; Lacticaseibacillus casei / growth & development; Lacticaseibacillus casei / metabolism
TL;DR:
An adhesive strain of L. casei was used to inoculate two packed-bed reactors, which operated with two mixed feedstock streams at the optimal conditions found above, and the highest overall l(+)-lactic acid purity was obtained in the two-chemostat system as compared with the immobilized-cell reactors.
(via Semantic Scholar)
Source: ORCID
Added: March 4, 2019
1999 journal article
Fluidized bed ion exchange for improving purification of lactic acid from fermentation.
Biotechnology Techniques., 3(3), 201–205.
Contributors: G. Raya-Tonetti*, P. Córdoba*, * , F. Siñeriz * & N. Perotti *
author keywords: fluidized bed; ion exchange; lactic acid
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: March 4, 2019
1998 journal article
Chemostat production of plantaricin C by <i>Lactobacillus plantarum</i> LL441
Applied and Environmental Microbiology. https://publons.com/wos-op/publon/28290537/
Contributors: J. Bárcena, F. Siñeriz, D. Llano, A. Rodríguez & J. Suárez
Source: ORCID
Added: May 4, 2020
1998 journal article
Chemostat production of plantaricin C by Lactobacillus plantarum LL441
Applied and Environmental Microbiology, 64(9), 3512–3514. http://www.scopus.com/inward/record.url?eid=2-s2.0-3543035172&partnerID=MN8TOARS
Contributors: J. Bruno Bárcena, F. Siñeriz, D. De González Llano, A. Rodríguez & J. Suárez
Source: ORCID
Added: May 4, 2020
1998 journal article
Chemostat production of plantaricin C by Lactobacillus plantarum LL441.
Applied and Environmental Microbiology, 9. http://europepmc.org/abstract/med/9726907
Source: ORCID
Added: March 4, 2019
1998 journal article
Fragile cells of Lactobacillus casei suitable for plasmid DNA isolation
Biotechnology Techniques. https://publons.com/publon/31684369/
Source: ORCID
Added: May 4, 2020
1998 journal article
Fragile cells of Lactobacillus casei suitable for plasmid DNA isolation.
Biotechnology Techniques., 2(2), 97–99.
TL;DR:
Atypical morphologies were very fragile since they were lysed by treatments with 1% SDS and 1mg/ml proteinase K but they were efficiently used for plasmids isolation without treatment with phenol.
(via Semantic Scholar)
Source: ORCID
Added: March 4, 2019
1998 journal article
Microbial physiology applied to process optimisation: Lactic acid bacteria
Advances in Bioprocess Engineering Volume Ii.
Contributors: * , A. Ragout* & F. Sineriz *
TL;DR:
The study of the interactions between the microorganism and its environment is what is usually recognised as microbial physiology and the same principle can be applied to any biotechnological process involving microorganims.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: May 4, 2020
1997 journal article
Shifts in pH affect the maltose/glycerol co-fermentation by Lactobacillus reuteri
Biotechnology Letters, 19(7), 645–649.
Contributors: G. deValdez, A. Ragout*, J. BrunoBarcena *, H. Diekmann & F. Sineriz *
Source: ORCID
Added: May 4, 2020
Employment
Updated: May 15th, 2020 17:57
2020 - present
North Carolina State University Raleigh, NC, US
Professor
Plant and Microbial Biology
2014 - 2020
North Carolina State University Raleigh, NC, US
Associated Professor
Plant and Microbial Biology
2007 - 2014
North Carolina State University Raleigh, NC, US
Assistant Profesor
Microbiology
Education
Updated: June 28th, 2020 11:52
1994 - 1997
Universidad Nacional de Tucumán San Miguel de Tucuman, AR
PhD
Facultad de Ciencias Naturales e Instituto Miguel Lillo
1985 - 1991
Universidad de Oviedo Oviedo, Asturias, ES
Functional Biology
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