@article{dewey_selote_griffin_dickey_jantz_smith_matthiadis_strable_kestell_smith_2023, title={Cytoplasmic male sterility and abortive seed traits generated through mitochondrial genome editing coupled with allotopic expression of atp1 in tobacco}, volume={14}, ISSN={["1664-462X"]}, DOI={10.3389/fpls.2023.1253640}, abstractNote={Allotopic expression is the term given for the deliberate relocation of gene function from an organellar genome to the nuclear genome. We hypothesized that the allotopic expression of an essential mitochondrial gene using a promoter that expressed efficiently in all cell types except those responsible for male reproduction would yield a cytoplasmic male sterility (CMS) phenotype once the endogenous mitochondrial gene was inactivated via genome editing. To test this, we repurposed the mitochondrially encoded atp1 gene of tobacco to function in the nucleus under the transcriptional control of a CaMV 35S promoter (construct 35S:nATP1), a promoter that has been shown to be minimally expressed in early stages of anther development. The endogenous atp1 gene was eliminated (Δatp1) from 35S:nATP1 tobacco plants using custom-designed meganucleases directed to the mitochondria. Vegetative growth of most 35S:nATP1/Δatp1 plants appeared normal, but upon flowering produced malformed anthers that failed to shed pollen. When 35S:nATP1/Δatp1 plants were cross-pollinated, ovary/capsule development appeared normal, but the vast majority of the resultant seeds were small, largely hollow and failed to germinate, a phenotype akin to the seedless trait known as stenospermocarpy. Characterization of the mitochondrial genomes from three independent Δatp1 events suggested that spontaneous recombination over regions of microhomology and substoichiometric shifting were the mechanisms responsible for atp1 elimination and genome rearrangement in response to exposure to the atp1-targeting meganucleases. Should the results reported here in tobacco prove to be translatable to other crop species, then multiple applications of allotopic expression of an essential mitochondrial gene followed by its elimination through genome editing can be envisaged. Depending on the promoter(s) used to drive the allotopic gene, this technology may have potential application in the areas of: (1) CMS trait development for use in hybrid seed production; (2) seedless fruit production; and (3) transgene containment.}, journal={FRONTIERS IN PLANT SCIENCE}, author={Dewey, Ralph E. and Selote, Devarshi and Griffin, H. Carol and Dickey, Allison N. and Jantz, Derek and Smith, J. Jeff and Matthiadis, Anna and Strable, Josh and Kestell, Caitlin and Smith, William A.}, year={2023}, month={Sep} }
 @article{strable_unger-wallace_raygoza_briggs_vollbrecht_2023, title={Interspecies transfer of RAMOSA1 orthologs and promoter cis sequences impacts maize inflorescence architecture}, volume={191}, ISSN={["1532-2548"]}, DOI={10.1093/plphys/kiac559}, abstractNote={Abstract}, number={2}, journal={PLANT PHYSIOLOGY}, author={Strable, Josh and Unger-Wallace, Erica and Raygoza, Alejandro Aragon and Briggs, Sarah and Vollbrecht, Erik}, year={2023}, month={Feb}, pages={1084–1101} }
 @article{laureyns_joossens_herwegh_pevernagie_pavie_demuynck_debray_coussens_pauwels_van hautegem_et al._2022, title={An in situ sequencing approach maps PLASTOCHRON1 at the boundary between indeterminate and determinate cells}, volume={188}, ISSN={["1532-2548"]}, DOI={10.1093/plphys/kiab533}, abstractNote={Abstract}, number={2}, journal={PLANT PHYSIOLOGY}, author={Laureyns, Reinout and Joossens, Jessica and Herwegh, Denia and Pevernagie, Julie and Pavie, Benjamin and Demuynck, Kirin and Debray, Kevin and Coussens, Griet and Pauwels, Laurens and Van Hautegem, Tom and et al.}, year={2022}, month={Feb}, pages={782–794} }
 @article{strable_2021, title={A pointillist portrait of maize leaf protoplasts points to bundle sheath polarity and a potentially new path to phloem loading}, volume={33}, ISSN={["1532-298X"]}, DOI={10.1093/plcell/koaa058}, number={3}, journal={PLANT CELL}, author={Strable, Josh}, year={2021}, month={Mar}, pages={447–448} }
 @article{strable_2021, title={Developmental genetics of maize vegetative shoot architecture}, volume={41}, ISSN={["1572-9788"]}, DOI={10.1007/s11032-021-01208-1}, abstractNote={More than 1.1 billion tonnes of maize grain were harvested across 197 million hectares in 2019 (FAOSTAT 2020). The vast global productivity of maize is largely driven by denser planting practices, higher yield potential per area of land, and increased yield potential per plant. Shoot architecture, the three-dimensional structural arrangement of the above-ground plant body, is critical to maize grain yield and biomass. Structure of the shoot is integral to all aspects of modern agronomic practices. Here, the developmental genetics of the maize vegetative shoot is reviewed. Plant architecture is ultimately determined by meristem activity, developmental patterning, and growth. The following topics are discussed: shoot apical meristem, leaf architecture, axillary meristem and shoot branching, and intercalary meristem and stem activity. Where possible, classical and current studies in maize developmental genetics, as well as recent advances leveraged by "-omics" analyses, are highlighted within these sections.The online version contains supplementary material available at 10.1007/s11032-021-01208-1.}, number={3}, journal={MOLECULAR BREEDING}, author={Strable, Josh}, year={2021}, month={Mar} }
 @article{washburn_strable_dickinson_kothapalli_brose_covshoff_conant_hibberd_pires_2021, title={Distinct C-4 sub-types and C-3 bundle sheath isolation in the Paniceae grasses}, volume={5}, ISSN={["2475-4455"]}, DOI={10.1002/pld3.373}, abstractNote={Abstract}, number={12}, journal={PLANT DIRECT}, author={Washburn, Jacob D. and Strable, Josh and Dickinson, Patrick and Kothapalli, Satya S. and Brose, Julia M. and Covshoff, Sarah and Conant, Gavin C. and Hibberd, Julian M. and Pires, Joseph Chris}, year={2021}, month={Dec} }
 @misc{abraham-juarez_barnes_aragon-raygoza_tyson_kur_strable_rellan-alvarez_2021, title={The arches and spandrels of maize domestication, adaptation, and improvement}, volume={64}, ISSN={["1879-0356"]}, url={https://doi.org/10.1016/j.pbi.2021.102124}, DOI={10.1016/j.pbi.2021.102124}, abstractNote={People living in the Balsas River basin in southwest México domesticated maize from the bushy grass teosinte. Nine thousand years later, in 2021, Ms. Deb Haaland - a member of the Pueblo of Laguna tribe of New Mexico - wore a dress adorned with a cornstalk when she was sworn in as the Secretary of Interior of the United States of America. This choice of garment highlights the importance of the coevolution of maize and the farmers who, through careful selection over thousands of years, domesticated maize and adapted the physiology and shoot architecture of maize to fit local environments and growth habits. Some traits such as tillering were directly selected on (arches), and others such as tassel size are the by-products (spandrels) of maize evolution. Here, we review current knowledge of the underlying cellular, developmental, physiological, and metabolic processes that were selected by farmers and breeders, which have positioned maize as a top global staple crop.}, journal={CURRENT OPINION IN PLANT BIOLOGY}, publisher={Elsevier BV}, author={Abraham-Juarez, Maria Jazmin and Barnes, Allison C. and Aragon-Raygoza, Alejandro and Tyson, Destiny and Kur, Andi and Strable, Josh and Rellan-Alvarez, Ruben}, year={2021}, month={Dec} }
 @misc{strable_nelissen_2021, title={The dynamics of maize leaf development: Patterned to grow while growing a pattern}, volume={63}, ISSN={["1879-0356"]}, DOI={10.1016/j.pbi.2021.102038}, abstractNote={Leaves are a significant component of the shoot system in grasses, functioning in light capture and photosynthesis. Leaf width, length, and angle are expressions of development that collectively define canopy architecture. Thus, the distinctive morphology of grass leaves is an interdependent readout of developmental patterning and growth along the proximal-distal, medial-lateral, and adaxial-abaxial axes. Here, we review the chronology of patterning and growth, namely along the proximal-distal axis, during maize leaf development. We underscore that patterning and growth occur simultaneously, making use of shared developmental gradients and molecular pathways.}, journal={CURRENT OPINION IN PLANT BIOLOGY}, author={Strable, Josh and Nelissen, Hilde}, year={2021}, month={Oct} }