@article{hurley-sanders_stoskopf_nelson_showers_law_gracz_levine_2015, title={Tissue extraction methods for metabolic profiling of a freshwater bivalve, Elliptio complanata}, volume={33}, ISSN={0740-2783 2162-2698}, url={http://dx.doi.org/10.4003/006.033.0209}, DOI={10.4003/006.033.0209}, abstractNote={Abstract: Much is still unknown about why freshwater mussels (Unionidae) are particularly sensitive to environmental change. A better understanding of freshwater mussel metabolism is needed, and the field of environmental metabolomics holds the promise to inform these questions. A number of protocols exist for the extraction of metabolites for identification from animal tissues. As a first step in the application of environmental metabolomics to the study of freshwater mussels, we compared extraction protocols using an inorganic oxidizing acid (perchloric acid), an organic nitrile (acetonitrile), and a salt/water solution (Ringer's solution) to establish an uncomplicated, robust, repeatable and inexpensive tissue extraction protocol for freshwater mussel tissue. Perchloric acid resulted in notable extraction of energy-related nucleotides (AMP/ADP/ATP), yet had the lowest peak count of the three extraction methods and showed poor repeatability. Acetonitrile and Ringer's solution yielded metabolite extraction results similar to each other with Ringer's solution having the greatest number of peaks particularly in the 3.0–4.5 ppm sugar/amino acid range. Ringer's solution is simple to use, safe and consistent and bears consideration when selecting an extraction protocol for 1H nuclear magnetic resonance experiments.}, number={2}, journal={American Malacological Bulletin}, publisher={American Malacological Society}, author={Hurley-Sanders, J.L. and Stoskopf, M. and Nelson, S.A.C. and Showers, W. and Law, J.M. and Gracz, H.S. and Levine, J.F.}, year={2015}, pages={185–194} } @article{hurley-sanders_harms_christiansen_clarke_law_2015, title={Exuberant granulation tissue response associated with Neobenedenia sp. (Monogenea: Capsalidae) infestation in two cobia, Rachycentron canadum (Linnaeus)}, volume={39}, ISSN={0140-7775}, url={http://dx.doi.org/10.1111/jfd.12360}, DOI={10.1111/jfd.12360}, abstractNote={AbstractMonogenean parasite infestations are common in captive marine teleosts, and are generally found on the skin and gills. This report describes an unusual pathological presentation of exuberant granulation tissue of the gills, suspected to be related to Neobenedenia infestation in two cobia housed together at a North Carolina aquarium.}, number={3}, journal={Journal of Fish Diseases}, publisher={Wiley}, author={Hurley-Sanders, J and Harms, C and Christiansen, E and Clarke, E, III and Law, J}, year={2015}, month={Apr}, pages={277–283} } @article{hurley-sanders_larsen_troan_loomis_2015, title={FUNGAL OSTEOMYELITIS IN TWO BUFFLEHEAD DUCKLINGS (BUCEPHALA ALBEOLA)}, volume={46}, ISSN={["1937-2825"]}, DOI={10.1638/2014-0196.1}, abstractNote={Abstract:  Over a 25-mo span from a single zoologic collection, two bufflehead ducklings (Bucephala albeola) presented with pelvic limb paresis and were euthanized. On postmortem examination, the first duckling had intralesional fungal hyphae consistent with Aspergillus sp. in the spinal vertebrae and within pulmonary granulomas. In the second duckling, evidence of a thoracic spinal lesion was detected antemortem by using thermographic imaging. At postmortem examination, fungal hyphae consistent with Mucor sp. were found within the vertebrae. Although fungal infections of the respiratory system are commonly reported in waterfowl, infections that involve the spinal cord and vertebrae are unusual. These cases highlight the importance of consideration of axial skeleton fungal disease in neurologic presentations and the use of thermography for noninvasive diagnostic screening.}, number={3}, journal={JOURNAL OF ZOO AND WILDLIFE MEDICINE}, author={Hurley-Sanders, Jennifer L. and Larsen, R. Scott and Troan, Brigid and Loomis, Michael}, year={2015}, month={Sep}, pages={613–616} } @article{hurley-sanders_sladky_nolan_loomis_2015, title={Use of cortical bone fenestration, autogenous free skin graft, and thermography for wound treatment and monitoring in a red wolf (Canis rufus gregoryi)}, volume={46}, DOI={10.1638/2014-0197.1}, abstractNote={Abstract:  A 2-yr-old female red wolf (Canis rufus gregoryi) sustained a degloving injury to the left thoracic limb while in a display habitat. Initial attempts to resolve the extensive wound by using conservative measures were unsuccessful. Subsequent treatment using a free skin graft consisted first of establishment of an adequate granulation bed via cortical bone fenestration. After establishment of a healthy granulation bed was achieved, free skin graft was harvested and transposed over the bed. To monitor viability and incorporation of the graft, serial thermographic imaging was performed. Thermography noninvasively detects radiant heat patterns and can be used to assess vascularization of tissue, potentially allowing early detection of graft failure. In this case, thermography documented successful graft attachment.}, number={3}, journal={Journal of Zoo and Wildlife Medicine}, author={Hurley-Sanders, J. L. and Sladky, K. K. and Nolan, E. C. and Loomis, M. R.}, year={2015}, pages={617–620} } @article{hurley-sanders_bowman_wolfe_nutter_sladky_stoskopf_2012, title={Use of Thermography and Fluorescein Angiography in the Management of a Chilean Flamingo With Avascular Necrosis of the Wing}, volume={26}, ISSN={["1938-2871"]}, DOI={10.1647/2011-018r.1}, abstractNote={Abstract A Chilean flamingo (Phoenicopterus chilensis) was presented to the veterinary clinic at the North Carolina Zoological Park for evaluation of acute weakness of the right wing. Results of a physical examination revealed a lack of a palpable pulse in the radial artery, which suggested occlusion or obstruction of the vessel. Radiography, thermography, and fluorescein angiography confirmed right wing injury and vascular compromise. Based on the poor prognosis for return to function associated with irreversible vascular damage, the wing was amputated. After a period of observation and treatment, the bird was returned to public exhibit.}, number={4}, journal={JOURNAL OF AVIAN MEDICINE AND SURGERY}, author={Hurley-Sanders, Jennifer L. and Bowman, Karl F. and Wolfe, Barbara A. and Nutter, Felicia B. and Sladky, Kurt K. and Stoskopf, Michael K.}, year={2012}, month={Dec}, pages={255–257} }