@article{lim_koh_thomas_breen_olby_2017, title={Evaluation of gene expression and DNA copy number profiles of adipose tissue-derived stromal cells and consecutive neurosphere-like cells generated from dogs with naturally occurring spinal cord injury}, volume={78}, ISSN={0002-9645}, url={http://dx.doi.org/10.2460/ajvr.78.3.371}, DOI={10.2460/ajvr.78.3.371}, abstractNote={Abstract}, number={3}, journal={American Journal of Veterinary Research}, publisher={American Veterinary Medical Association (AVMA)}, author={Lim, Ji-Hey and Koh, Sehwon and Thomas, Rachael and Breen, Matthew and Olby, Natasha J.}, year={2017}, month={Mar}, pages={371–380} }
 @article{blau_davis_gorney_dohse_williams_lim_pfitzner_laber_sawicki_olby_2017, title={Quantifying center of pressure variability in chondrodystrophoid dogs}, volume={226}, ISSN={1090-0233}, url={http://dx.doi.org/10.1016/j.tvjl.2017.07.001}, DOI={10.1016/j.tvjl.2017.07.001}, abstractNote={The center of pressure (COP) position reflects a combination of proprioceptive, motor and mechanical function. As such, it can be used to quantify and characterize neurologic dysfunction. The aim of this study was to describe and quantify the movement of COP and its variability in healthy chondrodystrophoid dogs while walking to provide a baseline for comparison to dogs with spinal cord injury due to acute intervertebral disc herniations. Fifteen healthy adult chondrodystrophoid dogs were walked on an instrumented treadmill that recorded the location of each dog’s COP as it walked. Center of pressure (COP) was referenced from an anatomical marker on the dogs’ back. The root mean squared (RMS) values of changes in COP location in the sagittal (y) and horizontal (x) directions were calculated to determine the range of COP variability. Three dogs would not walk on the treadmill. One dog was too small to collect interpretable data. From the remaining 11 dogs, 206 trials were analyzed. Mean RMS for change in COPx per trial was 0.0138 (standard deviation, SD 0.0047) and for COPy was 0.0185 (SD 0.0071). Walking speed but not limb length had a significant effect on COP RMS. Repeat measurements in six dogs had high test retest consistency in the x and fair consistency in the y direction. In conclusion, COP variability can be measured consistently in dogs, and a range of COP variability for normal chondrodystrophoid dogs has been determined to provide a baseline for future studies on dogs with spinal cord injury.}, journal={The Veterinary Journal}, publisher={Elsevier BV}, author={Blau, S.R. and Davis, L.M. and Gorney, A.M. and Dohse, C.S. and Williams, K.D. and Lim, J-H. and Pfitzner, W.G. and Laber, E. and Sawicki, G.S. and Olby, N.J.}, year={2017}, month={Aug}, pages={26–31} }
 @article{olby_muguet-chanoit_lim_davidian_mariani_freeman_platt_humphrey_kent_giovanella_et al._2016, title={A Placebo-Controlled, Prospective, Randomized Clinical Trial of Polyethylene Glycol and Methylprednisolone Sodium Succinate in Dogs with Intervertebral Disk Herniation}, volume={30}, ISSN={["1939-1676"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84956678975&partnerID=MN8TOARS}, DOI={10.1111/jvim.13657}, abstractNote={BackgroundAcute intervertebral disk herniation (IVDH) is a common cause of spinal cord injury in dogs and currently there is no proven medical treatment to counter secondary injury effects. Use of methylprednisolone sodium succinate (MPSS) or polyethylene glycol (PEG) as neuroprotectants is advocated but controversial because neither treatment has been tested in placebo‐controlled, randomized, blinded trials in dogs.}, number={1}, journal={JOURNAL OF VETERINARY INTERNAL MEDICINE}, author={Olby, N. J. and Muguet-Chanoit, A. C. and Lim, J. -H. and Davidian, M. and Mariani, C. L. and Freeman, A. C. and Platt, S. R. and Humphrey, J. and Kent, M. and Giovanella, C. and et al.}, year={2016}, pages={206–214} }
 @article{gorney_blau_dohse_griffith_williams_lim_knazovicky_lascelles_olby_2016, title={Mechanical and Thermal Sensory Testing in Normal Chondrodystrophoid Dogs and Dogs with Spinal Cord Injury caused by Thoracolumbar Intervertebral Disc Herniations}, volume={30}, ISSN={0891-6640}, url={http://dx.doi.org/10.1111/jvim.13913}, DOI={10.1111/jvim.13913}, abstractNote={BackgroundIntervertebral disc herniation is a common cause of spinal cord injury (SCI) causing paralysis and sensory loss. Little quantitative information is available on the loss and recovery of sensation in dogs with SCI.}, number={2}, journal={Journal of Veterinary Internal Medicine}, publisher={Wiley}, author={Gorney, A.M. and Blau, S.R. and Dohse, C.S. and Griffith, E.H. and Williams, K.D. and Lim, J.-H. and Knazovicky, D. and Lascelles, B.D.X. and Olby, N.J.}, year={2016}, month={Mar}, pages={627–635} }
 @article{olby_lim_babb_bach_domaracki_williams_griffith_harris_muguet-chanoit_2014, title={Gait scoring in dogs with thoracolumbar spinal cord injuries when walking on a treadmill}, volume={10}, journal={BMC Veterinary Research}, author={Olby, N. J. and Lim, J. H. and Babb, K. and Bach, K. and Domaracki, C. and Williams, K. and Griffith, E. and Harris, T. and Muguet-Chanoit, A.}, year={2014} }
 @article{lim_muguet-chanoit_smith_laber_olby_2014, title={Potassium channel antagonists 4-aminopyridine and the t-butyl carbamate derivative of 4-aminopyridine improve hind limb function in chronically non-ambulatory dogs; a blinded, placebo-controlled trial}, volume={9}, number={12}, journal={PLoS One}, author={Lim, J. H. and Muguet-Chanoit, A. C. and Smith, D. T. and Laber, E. and Olby, N. J.}, year={2014} }
 @article{lim_mccullen_piedrahita_loboa_olby_2013, title={Alternating Current Electric Fields of Varying Frequencies: Effects on Proliferation and Differentiation of Porcine Neural Progenitor Cells}, volume={15}, ISSN={2152-4971 2152-4998}, url={http://dx.doi.org/10.1089/cell.2013.0001}, DOI={10.1089/cell.2013.0001}, abstractNote={Application of sinusoidal electric fields (EFs) has been observed to affect cellular processes, including alignment, proliferation, and differentiation. In the present study, we applied low-frequency alternating current (AC) EFs to porcine neural progenitor cells (pNPCs) and investigated the effects on cell patterning, proliferation, and differentiation. pNPCs were grown directly on interdigitated electrodes (IDEs) localizing the EFs to a region accessible visually for fluorescence-based assays. Cultures of pNPCs were exposed to EFs (1 V/cm) of 1 Hz, 10 Hz, and 50 Hz for 3, 7, and 14 days and compared to control cultures. Immunocytochemistry was performed to evaluate the expression of neural markers. pNPCs grew uniformly with no evidence of alignment to the EFs and no change in cell numbers when compared with controls. Nestin expression was shown in all groups at 3 and 7 days, but not at 14 days. NG2 expression was low in all groups. Co-expression of glial fibrillary acidic protein (GFAP) and TUJ1 was significantly higher in the cultures exposed to 10- and 50-Hz EFs than the controls. In summary, sinusoidal AC EFs via IDEs did not alter the alignment and proliferation of pNPCs, but higher frequency stimulation appeared to delay differentiation into mature astrocytes.}, number={5}, journal={Cellular Reprogramming}, publisher={Mary Ann Liebert Inc}, author={Lim, Ji-Hey and McCullen, Seth D. and Piedrahita, Jorge A. and Loboa, Elizabeth G. and Olby, Natasha J.}, year={2013}, month={Oct}, pages={405–412} }
 @article{koh_thomas_tsai_bischoff_lim_breen_olby_piedrahita_2013, title={Growth Requirements and Chromosomal Instability of Induced Pluripotent Stem Cells Generated from Adult Canine Fibroblasts}, volume={22}, ISSN={1547-3287 1557-8534}, url={http://dx.doi.org/10.1089/scd.2012.0393}, DOI={10.1089/scd.2012.0393}, abstractNote={In mice and humans, it has been shown that embryonic and adult fibroblasts can be reprogrammed into pluripotency by introducing 4 transcription factors, Oct3/4, Klf4, Sox2, and c-Myc (OKSM). Here, we report the derivation of induced pluripotent stem cells (iPSCs) from adult canine fibroblasts by retroviral OKSM transduction. The isolated canine iPSCs (ciPSCs) were expanded in 3 different culture media [fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), or FGF2 plus LIF]. Cells cultured in both FGF2 and LIF expressed pluripotency markers [POU5F1 (OCT4), SOX2, NANOG, and LIN28] and embryonic stem cell (ESC)-specific genes (PODXL, DPPA5, FGF5, REX1, and LAMP1) and showed strong levels of alkaline phosphatase expression. In vitro differentiation by formation of embryoid bodies and by directed differentiation generated cell derivatives of all 3 germ layers as confirmed by mRNA and protein expression. In vivo, the ciPSCs created solid tumors, which failed to reach epithelial structure formation, but expressed markers for all 3 germ layers. Array comparative genomic hybridization and chromosomal fluorescence in situ hybridization analyses revealed that while retroviral transduction per se did not result in significant DNA copy number imbalance, there was evidence for the emergence of low-level aneuploidy during prolonged culture or tumor formation. In summary, we were able to derive ciPSCs from adult fibroblasts by using 4 transcription factors. The isolated iPSCs have similar characteristics to ESCs from other species, but the exact cellular mechanisms behind their unique co-dependency on both FGF2 and LIF are still unknown.}, number={6}, journal={Stem Cells and Development}, publisher={Mary Ann Liebert Inc}, author={Koh, Sehwon and Thomas, Rachael and Tsai, Shengdar and Bischoff, Steve and Lim, Ji-Hey and Breen, Matthew and Olby, Natasha J. and Piedrahita, Jorge A.}, year={2013}, month={Mar}, pages={951–963} }
 @inproceedings{koh_tsai_bischoff_thomas_lim_breen_olby_piedrahita_2012, title={Generation and characterization of induced pluripotent stem cells (iPS) from adult canine fibroblasts}, volume={47}, booktitle={Reproduction in Domestic Animals}, author={Koh, S. and Tsai, S. and Bischoff, S. and Thomas, R. and Lim, J. H. and Breen, M. and Olby, N. and Piedrahita, J.}, year={2012}, pages={418–419} }
 @article{lim_koh_olby_piedrahita_mariani_2012, title={Isolation and characterization of neural progenitor cells from adult canine brains}, volume={73}, ISSN={0002-9645}, url={http://dx.doi.org/10.2460/ajvr.73.12.1963}, DOI={10.2460/ajvr.73.12.1963}, abstractNote={Abstract}, number={12}, journal={American Journal of Veterinary Research}, publisher={American Veterinary Medical Association (AVMA)}, author={Lim, Ji-Hey and Koh, Sehwon and Olby, Natasha J. and Piedrahita, Jorge and Mariani, Christopher L.}, year={2012}, month={Dec}, pages={1963–1968} }
 @article{muguet-chanoit_olby_lim_gallagher_niman_dillard_campbell_early_mariani_muñana_et al._2011, title={The Cutaneous Trunci Muscle Reflex: A Predictor of Recovery in Dogs with Acute Thoracolumbar Myelopathies Caused by Intervertebral Disc Extrusions}, volume={41}, ISSN={0161-3499}, url={http://dx.doi.org/10.1111/j.1532-950X.2011.00921.x}, DOI={10.1111/j.1532-950x.2011.00921.x}, abstractNote={ObjectiveTo determine whether changes in the cutaneous trunci muscle (CTM) reflex are an early predictor of outcome in dogs with severe acute thoracolumbar intervertebral disc extrusion (IVDE).}, number={2}, journal={Veterinary Surgery}, publisher={Wiley}, author={Muguet-Chanoit, Audrey C. and Olby, Natasha J. and Lim, Ji-Hey and Gallagher, Ryan and Niman, Zachary and Dillard, Stacy and Campbell, James and Early, Peter and Mariani, Christopher L. and Muñana, Karen R. and et al.}, year={2011}, month={Dec}, pages={200–206} }
 @article{muguet-chanoit_olby_babb_lim_gallagher_niman_dillard_campbell_2011, title={The sensory field and repeatability of the cutaneous trunci muscle reflex of the dog}, volume={40}, number={7}, journal={Veterinary Surgery}, author={Muguet-Chanoit, A. C. and Olby, N. J. and Babb, K. M. and Lim, J. H. and Gallagher, R. and Niman, Z. and Dillard, S. and Campbell, J.}, year={2011}, pages={781–785} }
 @article{lim_piedrahita_jackson_ghashghaei_olby_2010, title={Development of a Model of Sacrocaudal Spinal Cord Injury in Cloned Yucatan MiniPigs for Cellular Transplantation Research}, volume={12}, ISSN={["2152-4998"]}, DOI={10.1089/cell.2010.0039}, abstractNote={Research into transplantation strategies to treat spinal cord injury (SCI) is frequently performed in rodents, but translation of results to clinical patients can be poor and a large mammalian model of severe SCI is needed. The pig has been considered an optimal model species in which to perform preclinical testing, and the Yucatan minipig can be cloned successfully utilizing somatic cell nuclear transfer (SCNT). However, induction of paralysis in pigs poses significant welfare and nursing challenges. The present study was conducted to determine whether Yucatan SCNT clones could be used to develop an SCI animal model for cellular transplantation research. First, we demonstrated that transection of the sacrocaudal spinal cord in Yucatan SCNT clones produces profound, quantifiable neurological deficits restricted to the tail. We then established that neurospheres could be isolated from brain tissue of green fluorescence protein (GFP) transfected SCNT clones. Finally, we confirmed survival of transplanted GFP-expressing neural stem cells in the SCI lesion and their differentiation into glial and neuronal lineages for up to 4 weeks without immunosuppression. We conclude that this model of sacrocaudal SCI in Yucatan SCNT clones represents a powerful research tool to investigate the effect of cellular transplantation on axonal regeneration and functional recovery.}, number={6}, journal={CELLULAR REPROGRAMMING}, author={Lim, Ji-Hey and Piedrahita, Jorge A. and Jackson, Lauren and Ghashghaei, Troy and Olby, Natasha J.}, year={2010}, month={Dec}, pages={689–697} }