@article{risselada_tuohy_law_james_lascelles_2020, title={Local Administration of Carboplatin in Poloxamer 407 After an Ulnar Osteosarcoma Removal in a Dog}, volume={56}, ISSN={["1547-3317"]}, DOI={10.5326/JAAHA-MS-6926}, abstractNote={ABSTRACT
               An 8 yr old male castrated hound presented for a left distal ulnar osteosarcoma. Staging (computed tomography and nuclear scintigraphy) did not reveal any metastases. A limb-sparing ulnectomy with local adjunctive carboplatin in a poloxamer copolymer gel (poloxamer 407) was performed. The patient recovered without complications after surgery. No wound healing complications or adverse effects occurred after local use of carboplatin in poloxamer 407. The local recurrence-free interval was 296 days from surgery, and the survival time was 445 days from initial diagnosis. This is the first report in the veterinary literature of using poloxamer 407 as a carrier for local delivery of chemotherapeutic drugs in a clinical patient.}, number={6}, journal={JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION}, author={Risselada, Marije and Tuohy, Joanne L. and Law, Mac and James, Mindi L. and Lascelles, B. Duncan X.}, year={2020}, pages={325–330} }
 @article{tuohy_lascelles_griffith_fogle_2016, title={Association of Canine Osteosarcoma and Monocyte Phenotype and Chemotactic Function}, volume={30}, ISSN={0891-6640}, url={http://dx.doi.org/10.1111/jvim.13983}, DOI={10.1111/jvim.13983}, abstractNote={BackgroundMonocytes/macrophages are likely key cells in immune modulation in dogs with osteosarcoma (OSA). Increased peripheral monocyte counts are negatively correlated with shorter disease‐free intervals in dogs with OSA. Understanding the monocyte/macrophage's modulatory role in dogs with OSA can direct further studies in immunotherapy development for OSA.Hypothesis/ObjectivesThat OSA evades the immune response by down‐regulating monocyte chemokine receptor expression and migratory function, and suppresses host immune responses.AnimalsEighteen dogs with OSA that have not received definitive treatment and 14 healthy age‐matched controlsMethodsClinical study—expression of peripheral blood monocyte cell surface receptors, monocyte mRNA expression and cytokine secretion, monocyte chemotaxis, and survival were compared between clinical dogs with OSA and healthy control dogs.ResultsCell surface expression of multiple chemokine receptors is significantly down‐regulated in peripheral blood monocytes of dogs with OSA. The percentage expression of CCR2 (median 58%, range 2–94%) and CXCR2 expression (median 54%, range 2–92%) was higher in control dogs compared to dogs with OSA (CCR2 median 29%, range 3–45%, P = 0.0006; CXCR2 median 23%, range 0.2–52%, P = 0.0007). Prostaglandin E2 (PGE2) (OSA, median 347.36 pg/mL, range 103.4–1268.5; control, 136.23 pg/mL, range 69.93–542.6, P = .04) and tumor necrosis factor‐alpha (TNF‐α) (P = .02) levels are increased in OSA monocyte culture supernatants compared to controls. Peripheral blood monocytes of dogs with OSA exhibit decreased chemotactic function when compared to control dogs (OSA, median 1.2 directed to random migration, range 0.8–1.25; control, 1.6, range of 0.9–1.8, P = .018).Conclusions and Clinical ImportanceDogs with OSA have decreased monocyte chemokine receptor expression and monocyte chemotaxis, potential mechanisms by which OSA might evade the immune response. Reversal of monocyte dysfunction using immunotherapy could improve survival in dogs with OSA.}, number={4}, journal={Journal of Veterinary Internal Medicine}, publisher={Wiley}, author={Tuohy, J.L. and Lascelles, B.D.X. and Griffith, E.H. and Fogle, J.E.}, year={2016}, month={Jun}, pages={1167–1178} }
 @article{tuohy_marcellin-little_griffith_2014, title={Durations of Bone Consolidation and External Fixation After Distraction Osteogenesis in Dogs}, volume={43}, ISSN={["1532-950X"]}, DOI={10.1111/j.1532-950x.2014.12189.x}, abstractNote={ObjectiveTo evaluate the influence of epidemiologic, surgical, and mechanical factors on the durations of bone consolidation and external fixation after distraction osteogenesis in dogs.Study DesignRetrospective cohort study.Sample PopulationDogs (n = 115) that had corrective osteotomy with circular external fixation (CEF; n = 152)MethodsMedical and radiographic records (1992–2012) of dogs that had corrective osteotomies were reviewed. Putative variables included age, weight, gender, and breed. Surgery date, delay before distraction, rate and duration of distraction, mechanical complications, and frame removal date were recorded. Radiographic data included bone operated, bone length, osteotomy site, bone and limb size at osteotomy site, distraction distance, and CEF frame size and stiffness.ResultsMean ± SD bone consolidation period was 56 ± 33 days. Mean duration of external fixation was 77 ± 35 days. Twelve fixation failures occurred. Radii healed faster than tibiae (P < .001). Failure of fixation (P = .002) and stiff frames (P = .033) increased duration of bone consolidation. For the tibia, durations of bone consolidation and external fixation decreased with larger bone size relative to limb size (P = .004). For the radius, bone consolidation duration decreased as distraction amount increased (P = .03).ConclusionRadii healed faster than tibiae. Wearing frames with low or moderate stiffness, the absence of mechanical complications, a larger distraction distance, and a larger bone size accelerated bone consolidation. Optimizing these factors should accelerate bone consolidation and reduce the duration of external fixation.}, number={8}, journal={VETERINARY SURGERY}, author={Tuohy, Joanne L. and Marcellin-Little, Denis J. and Griffith, Emily H.}, year={2014}, month={Nov}, pages={903–911} }