@article{escudero-abarca_goulter_bradshaw_faircloth_leslie_manuel_arbogast_jaykus_2022, title={Efficacy of an alcohol-based surface disinfectant formulation against human norovirus}, ISSN={["1365-2672"]}, DOI={10.1111/jam.15479}, abstractNote={Abstract}, journal={JOURNAL OF APPLIED MICROBIOLOGY}, author={Escudero-Abarca, Blanca I and Goulter, Rebecca M. and Bradshaw, Justin and Faircloth, Jeremy and Leslie, Rachel A. and Manuel, Clyde S. and Arbogast, James W. and Jaykus, Lee-Ann}, year={2022}, month={Feb} } @misc{sikes_mcmillan_bradshaw_2010, title={The Center of Accessibility: D beta Control of V(D)J Recombination}, volume={58}, ISSN={["0004-069X"]}, DOI={10.1007/s00005-010-0101-2}, abstractNote={Developmental patterning of antigen receptor gene assembly in lymphocyte precursors correlates with decondensation of the chromatin surrounding individual gene segments. Ongoing V(D)J recombination is associated with hyperacetylation of histones H3 and H4 and the expression of sterile germline transcripts across the region of recombinational accessibility. Likewise, histone acetyltransferase and SWI/SNF chromatin remodeling complexes each appear to be required for recombination, and the PHD-finger of RAG-2 preferentially associates with recombination signal sequence (RSS) chromatin that contains H3 trimethylated on lysine 4. However, the regulatory mechanisms that direct chromatin alteration and rearrangement have proven elusive, due in large part to the interdependency of individual stages in gene activation, our limited understanding of functional significance of changes to the histone code, and the difficulty of modeling recombinational accessibility in existing experimental systems. Examining Tcrb assembly in developing thymocytes, we review the central roles of RSS elements and germline promoters as foci for epigenetic reorganization of recombinationally accessible gene segments in light of recent findings and persistent questions.}, number={6}, journal={ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS}, author={Sikes, Michael L. and McMillan, Ruth E. and Bradshaw, Justin M.}, year={2010}, month={Dec}, pages={427–433} } @article{sikes_bradshaw_ivory_lunsford_mcmillan_morrison_2009, title={A streamlined method for rapid and sensitive chromatin immunoprecipitation}, volume={344}, ISSN={["0022-1759"]}, DOI={10.1016/j.jim.2009.03.007}, abstractNote={We report a streamlined procedure to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h. We use this streamlined ChIP to quantify histone H3 modifications at active (cad) and repressed (T early alpha) promoters in a Rag1-deficient pro-T cell line after 1–2 h IP. We further show that the protocol readily quantified histone modifications in chromatin from 104 Rag-deficient DN thymocytes. Taken together, these data outline a simple, cost-effective procedure for efficient ChIP analysis.}, number={1}, journal={JOURNAL OF IMMUNOLOGICAL METHODS}, author={Sikes, Michael L. and Bradshaw, Justin M. and Ivory, Wendell T. and Lunsford, Jessica L. and McMillan, Ruth E. and Morrison, Clayton R.}, year={2009}, month={May}, pages={58–63} }