@article{cheng_campler_schroeder_yang_mor_ferreira_arruda_2022, title={Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms}, volume={9}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2022.884733}, abstractNote={The detection and co-circulation of multiple variants of porcine reproductive and respiratory syndrome virus (PRRSV) have been observed and reported in swine. However, the potential long-term impact of multiple prevailing PRRSV variants on pig-performance is not yet fully understood. The primary objective of this study was to describe the genetic variation of PRRSV in processing fluid (PF), oral fluid (OF), and tonsil scraping (TS) specimens from five swine farms with different production types and PRRS status over a period of time (~1 year). Furthermore, the association between PRRSV prevalence and production parameters was investigated. Results showed that PRRSV was detected by RT-qPCR in 21–25% of all types of specimens. In breeding farms, PRRSV detection in PF and/or TS samples was correlated with stillborn and mummified fetuses, and pre-weaning mortality throughout the study period. Although ORF5 sequences were obtained in <16% of all sample types, simultaneous detection of PRRSV variants including field and vaccine strains within a single sampling event was identified in both breeding and growing pig farms. Phylogenetic analyses based on the ORF5 sequence classified the detected field PRRSV into L1A and L1H, two sub-lineages of lineage 1 (L1). Our study demonstrated the presence of multiple PRRSV lineages, sub-lineages, and variants in swine herds and its potential association with swine reproductive performance under field conditions.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Cheng, Ting-Yu and Campler, Magnus R. and Schroeder, Declan C. and Yang, My and Mor, Sunil K. and Ferreira, Juliana B. and Arruda, Andreia G.}, year={2022}, month={Jun} }
 @article{ferreira_anderson_correa_lyman_ruffin_reller_fowler_2011, title={Transmission of MRSA between Companion Animals and Infected Human Patients Presenting to Outpatient Medical Care Facilities}, volume={6}, ISSN={["1932-6203"]}, DOI={10.1371/journal.pone.0026978}, abstractNote={Methicillin-resistant Staphylococcus aureus (MRSA) is a significant pathogen in both human and veterinary medicine. The importance of companion animals as reservoirs of human infections is currently unknown. The companion animals of 49 MRSA-infected outpatients (cases) were screened for MRSA carriage, and their bacterial isolates were compared with those of the infected patients using Pulsed-Field Gel Electrophoresis (PFGE). Rates of MRSA among the companion animals of MRSA-infected patients were compared to rates of MRSA among companion animals of pet guardians attending a “veterinary wellness clinic” (controls). MRSA was isolated from at least one companion animal in 4/49 (8.2%) households of MRSA-infected outpatients vs. none of the pets of the 50 uninfected human controls. Using PFGE, patient-pets MRSA isolates were identical for three pairs and discordant for one pair (suggested MRSA inter-specie transmission p-value = 0.1175). These results suggest that companion animals of MRSA-infected patients can be culture-positive for MRSA, representing a potential source of infection or re-infection for humans. Further studies are required to better understand the epidemiology of MRSA human-animal inter-specie transmission.}, number={11}, journal={PLOS ONE}, author={Ferreira, Jorge Pinto and Anderson, Kevin L. and Correa, Maria T. and Lyman, Roberta and Ruffin, Felicia and Reller, L. Barth and Fowler, Vance G., Jr.}, year={2011}, month={Nov} }