J Michael Ramsey

Works (3)

Updated: July 5th, 2023 15:46

2017 article

N-Gemini peptides: cytosolic protease resistance via N-terminal dimerization of unstructured peptides

Fayer, E. L., Gilliland, W. M., Ramsey, J. M., Allbritton, N. L., & Waters, M. L. (2017, December 12). Chemical Communications.

By: E. Fayer*, W. Gilliland *, J. Ramsey n, N. Allbritton n & M. Waters *

MeSH headings : Cell Line; Cytosol / enzymology; Dimerization; HeLa Cells; Humans; Molecular Structure; Peptide Hydrolases / metabolism; Peptides / chemical synthesis; Peptides / chemistry; Peptides / pharmacology; Protease Inhibitors / chemistry; Protease Inhibitors / pharmacology
topics (OpenAlex): Peptidase Inhibition and Analysis; Chemical Synthesis and Analysis; Glycosylation and Glycoproteins Research
TL;DR: A synthetically simple strategy for increasing the lifetime of unstructured peptides in cytosolic environment via dimerization at the N-terminus to block threading into the catalytic cleft of cytosolics proteases is described. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2012 article

Laser-based directed release of array elements for efficient collection into targeted microwells

Dobes, N. C., Dhopeshwarkar, R., Henley, W. H., Ramsey, J. M., Sims, C. E., & Allbritton, N. L. (2012, November 28). The Analyst.

By: N. Dobes*, R. Dhopeshwarkar*, W. Henley*, J. Ramsey n, C. Sims * & N. Allbritton n

MeSH headings : Cell Line, Tumor; Cell Separation; Cell Survival; Dimethylpolysiloxanes / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; HeLa Cells; Humans; Lasers; Tissue Array Analysis; Transfection
topics (OpenAlex): 3D Printing in Biomedical Research; Microfluidic and Bio-sensing Technologies; Innovative Microfluidic and Catalytic Techniques Innovation
TL;DR: An array of microfabricated, releasable, transparent micron-scale pedestals termed pallets and an array of microwells in poly(dimethylsiloxane) (PDMS) were mated to enable selective release and retrieval of individual cells. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2012 article

Microfluidic Cytometer for the Characterization of Cell Lysis

SooHoo, J. R., Herr, J. K., Ramsey, J. M., & Walker, G. M. (2012, January 10). Analytical Chemistry.

By: J. SooHoo n, J. Herr*, J. Ramsey* & G. Walker n

MeSH headings : Diffusion; Erythrocytes / cytology; Hemolysis; Humans; Kinetics; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods; Quaternary Ammonium Compounds / chemistry
topics (OpenAlex): Microfluidic and Bio-sensing Technologies; Microfluidic and Capillary Electrophoresis Applications; Electrowetting and Microfluidic Technologies
TL;DR: A microfluidic cytometer was fabricated and achieved complete erythrocyte lysis of a blood sample in 0.7 s under optimum conditions, and a maximum lysis reaction rate of 1.55 s(-1) was extrapolated from the data. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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