@article{fayer_gilliland_ramsey_allbritton_waters_2018, title={N-Gemini peptides: cytosolic protease resistance via N-terminal dimerization of unstructured peptides}, volume={54}, ISSN={["1364-548X"]}, DOI={10.1039/c7cc06819k}, abstractNote={N-Terminal dimerization is a synthetically straight-forward strategy to provide protease resistance to unstructured peptides while maintaining their biological function.}, number={2}, journal={CHEMICAL COMMUNICATIONS}, author={Fayer, Effrat L. and Gilliland, William M., Jr. and Ramsey, J. Michael and Allbritton, Nancy L. and Waters, Marcey L.}, year={2018}, month={Jan}, pages={204–207} }
 @article{dobes_dhopeshwarkar_henley_ramsey_sims_allbritton_2013, title={Laser-based directed release of array elements for efficient collection into targeted microwells}, volume={138}, ISSN={["1364-5528"]}, DOI={10.1039/c2an36342a}, abstractNote={A cell separation strategy capable of the systematic isolation and collection of moderate to large numbers (25-400) of single cells into a targeted microwell is demonstrated. An array of microfabricated, releasable, transparent micron-scale pedestals termed pallets and an array of microwells in poly(dimethylsiloxane) (PDMS) were mated to enable selective release and retrieval of individual cells. Cells cultured on a pallet array mounted on a custom designed stage permitted the array to be positioned independently of the microwell locations. Individual pallets containing cells were detached in a targeted fashion using a pulsed Nd:YAG laser. The location of the laser focal point was optimized to transfer individual pallets to designated microwells. In a large-scale sort (n = 401), the accuracy, defined as placing a pallet in the intended well, was 94% and the collection efficiency was 100%. Multiple pallets were observed in only 4% of the targeted wells. In cell sorting experiments, the technique provided a yield and purity of target cells identified by their fluorescence signature of 91% and 93%, respectively. Cell viability based on single-cell cloning efficiency at 72 h post collection was 77%.}, number={3}, journal={ANALYST}, author={Dobes, Nicholas C. and Dhopeshwarkar, Rahul and Henley, W. Hampton and Ramsey, J. Michael and Sims, Christopher E. and Allbritton, Nancy L.}, year={2013}, pages={831–838} }
 @article{soohoo_herr_ramsey_walker_2012, title={Microfluidic Cytometer for the Characterization of Cell Lysis}, volume={84}, ISSN={["0003-2700"]}, DOI={10.1021/ac202461h}, abstractNote={Blood cytometry and intercellular analysis typically requires lysis as a preparatory step, which can alter the results of downstream analyses. We fabricated a microfluidic cytometer to characterize erythrocyte lysis kinetics. Forward light scatter from erythrocytes was used for enumeration at specific locations on a microfluidic chip. Diffusive transport coupled with laminar flow was used to control the concentration and exposure time of the lysis reagent Zap-OGLOBIN II to erythrocytes. Standard clinical practice is to expose erythrocytes to lysis reagent for 10 min. Under optimum conditions, we achieved complete erythrocyte lysis of a blood sample in 0.7 s. A maximum lysis reaction rate of 1.55 s(-1) was extrapolated from the data. Lysis began after 0.2 s and could be initiated with a lysis reagent concentration of 1.0% (68.5 mM). An equation that related lysis reagent concentration, [A], to erythrocyte lysis, [B], was determined to be [B] = -0.77[A](0.29)t.}, number={5}, journal={ANALYTICAL CHEMISTRY}, author={SooHoo, Jeffrey R. and Herr, Joshua K. and Ramsey, J. Michael and Walker, Glenn M.}, year={2012}, month={Mar}, pages={2195–2201} }