Works (4)

Updated: July 5th, 2023 15:30

2022 journal article

Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts


By: E. Jensen*, M. Deichmann*, X. Ma*, R. Vilandt*, G. Schiesaro*, M. Rojek*, B. Lengger*, L. Eliasson* ...

MeSH headings : Saccharomyces cerevisiae / metabolism; Reproduction / genetics; Receptors, G-Protein-Coupled / metabolism; Pheromones / metabolism; Receptors, Cell Surface / metabolism; Cell Differentiation; Cell Communication; Probiotics; Ligands
TL;DR: This work reports transcriptome profiles following mating pathway activation in native and hGPCR-signaling yeast, and uses a model-guided approach to correlate gene expression to morphological changes, and devise a ligand-free screening strategy for hGpcR compatibility with the yeast mating pathway and enable hG PCR-Signaling in the probiotic yeast Saccharomyces boulardii. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: October 31, 2022

2020 journal article

Competitive Exclusion Is a Major Bioprotective Mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products


author keywords: lactic acid bacteria; bioprotection; food spoilage; manganese starvation; genome editing; Lactobacillus
MeSH headings : Cultured Milk Products / microbiology; Food Microbiology; Fungi / physiology; Lactobacillus / physiology; Yeasts / physiology
TL;DR: Manganese scavenging emerges as a common trait within the Lactobacillus genus, but differences in expression result in some strains showing more bioprotective effect than others, which constitutes an attractive alternative to artificial preservation. (via Semantic Scholar)
Source: Web Of Science
Added: April 20, 2020

2019 review

Barriers to genome editing with CRISPR in bacteria


By: J. Vento n, N. Crook n & C. Beisel n

author keywords: Bacteria; Nuclease; Genome editing; CRISPR; Recombineering
MeSH headings : Bacteria / genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Editing; Humans
TL;DR: Existing barriers to implementing CRISPR-based editing across diverse bacterial species are analyzed and guidelines to help achieve and enhance editing in a wider range of bacterial species, including non-model strains are offered. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 28, 2019

2019 journal article

Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods


author keywords: genome editing; lactobacilli; recombineering; shuttle plasmids
MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Gene Editing / methods; Lactobacillus plantarum / genetics; Plasmids / genetics; Recombinases / genetics; Recombination, Genetic / genetics
TL;DR: Surprising differences for Cas9-mediated genome editing between methods and related strains are highlighted, arguing for the need for multiple, distinct methods when performing CRISPR-based editing in bacteria. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: March 18, 2019

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