Justin Vento

Works (6)

Updated: March 6th, 2026 15:08

2025 article

Cas9-independent tracrRNA cytotoxicity in Lacticaseibacillus paracasei

Arifah, A. Q., Vento, J. M., Kurrer, I., Achmedov, T., & Beisel, C. L. (2025, January 1). MicroLife.

By: A. Arifah *, J. Vento n, I. Kurrer*, T. Achmedov* & C. Beisel *

topics (OpenAlex): CRISPR and Genetic Engineering; RNA and protein synthesis mechanisms; Bacterial Genetics and Biotechnology
Source: NC State University Libraries
Added: March 4, 2026

2024 article

A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria

Vento, J. M., Durmusoglu, D., Li, T., Patinios, C., Sullivan, S., Ttofali, F., … Beisel, C. L. (2024, June 26). Molecular Cell, Vol. 84.

By: J. Vento n, D. Durmusoglu n, T. Li n, C. Patinios *, S. Sullivan  n, F. Ttofali n, J. Schaik n, Y. Yu * ...

MeSH headings : Transcription, Genetic; Cell-Free System; DNA Methylation; Protein Biosynthesis; Escherichia coli / genetics; Escherichia coli / metabolism; Transformation, Bacterial; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Plasmids / genetics; Plasmids / metabolism; DNA Modification Methylases / metabolism; DNA Modification Methylases / genetics; Bacterial Proteins / genetics; Bacterial Proteins / metabolism
topics (OpenAlex): Bacterial Genetics and Biotechnology; Bacteriophages and microbial interactions; CRISPR and Genetic Engineering
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Sources: Web Of Science, NC State University Libraries
Added: September 16, 2024

2022 article

Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts

Jensen, E. D., Deichmann, M., Ma, X., Vilandt, R. U., Schiesaro, G., Rojek, M. B., … Jensen, M. K. (2022, October 19). Nature Communications, Vol. 10.

By: E. Jensen *, M. Deichmann *, X. Ma *, R. Vilandt*, G. Schiesaro *, M. Rojek*, B. Lengger *, L. Eliasson* ...

MeSH headings : Saccharomyces cerevisiae / metabolism; Reproduction / genetics; Receptors, G-Protein-Coupled / metabolism; Pheromones / metabolism; Receptors, Cell Surface / metabolism; Cell Differentiation; Cell Communication; Probiotics; Ligands
topics (OpenAlex): Fungal and yeast genetics research; Plant Reproductive Biology; Yeasts and Rust Fungi Studies
TL;DR: This work reports transcriptome profiles following mating pathway activation in native and hGPCR-signaling yeast, and uses a model-guided approach to correlate gene expression to morphological changes, and devise a ligand-free screening strategy for hGpcR compatibility with the yeast mating pathway and enable hG PCR-Signaling in the probiotic yeast Saccharomyces boulardii. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: October 31, 2022

2020 article

Competitive Exclusion Is a Major Bioprotective Mechanism of Lactobacilli against Fungal Spoilage in Fermented Milk Products

Siedler, S., Rau, M. H., Bidstrup, S., Vento, J. M., Aunsbjerg, S. D., Bosma, E. F., … Neves, A. R. (2020, February 3). Applied and Environmental Microbiology.

By: S. Siedler *, M. Rau *, S. Bidstrup*, J. Vento n, S. Aunsbjerg *, E. Bosma *, L. McNair *, C. Beisel n, A. Neves *

author keywords: lactic acid bacteria; bioprotection; food spoilage; manganese starvation; genome editing; Lactobacillus
MeSH headings : Cultured Milk Products / microbiology; Food Microbiology; Fungi / physiology; Lactobacillus / physiology; Yeasts / physiology
topics (OpenAlex): Probiotics and Fermented Foods; Gut microbiota and health; Polyamine Metabolism and Applications
TL;DR: Manganese scavenging emerges as a common trait within the Lactobacillus genus, but differences in expression result in some strains showing more bioprotective effect than others, which constitutes an attractive alternative to artificial preservation. (via Semantic Scholar)
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Source: Web Of Science
Added: April 20, 2020

2019 article

Barriers to genome editing with CRISPR in bacteria

Vento, J. M., Crook, N., & Beisel, C. L. (2019, June 5). Journal of Industrial Microbiology & Biotechnology, Vol. 46, pp. 1327–1341.

By: J. Vento n, N. Crook n  & C. Beisel n

author keywords: Bacteria; Nuclease; Genome editing; CRISPR; Recombineering
MeSH headings : Bacteria / genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Editing; Humans
topics (OpenAlex): CRISPR and Genetic Engineering; Innovation and Socioeconomic Development; Insect symbiosis and bacterial influences
TL;DR: Existing barriers to implementing CRISPR-based editing across diverse bacterial species are analyzed and guidelines to help achieve and enhance editing in a wider range of bacterial species, including non-model strains are offered. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 28, 2019

2018 article

Genome Editing with CRISPR‐Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods

Leenay, R. T., Vento, J. M., Shah, M., Martino, M. E., Leulier, F., & Beisel, C. L. (2018, August 30). Biotechnology Journal.

By: R. Leenay*, J. Vento*, M. Shah*, M. Martino *, F. Leulier * & C. Beisel*

author keywords: genome editing; lactobacilli; recombineering; shuttle plasmids
MeSH headings : CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Gene Editing / methods; Lactobacillus plantarum / genetics; Plasmids / genetics; Recombinases / genetics; Recombination, Genetic / genetics
topics (OpenAlex): CRISPR and Genetic Engineering; Bacterial Genetics and Biotechnology; RNA and protein synthesis mechanisms
TL;DR: Surprising differences for Cas9-mediated genome editing between methods and related strains are highlighted, arguing for the need for multiple, distinct methods when performing CRISPR-based editing in bacteria. (via Semantic Scholar)
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Source: Web Of Science
Added: March 18, 2019

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