@article{detwiler_dobes_sims_kornegay_allbritton_2012, title={Polystyrene-coated micropallets for culture and separation of primary muscle cells}, volume={402}, ISSN={["1618-2642"]}, DOI={10.1007/s00216-011-5596-9}, abstractNote={Despite identification of a large number of adult stem cell types, current primary cell isolation and identification techniques yield heterogeneous samples, making detailed biological studies challenging. To identify subsets of isolated cells, technologies capable of simultaneous cell culture and cloning are necessary. Micropallet arrays, a new cloning platform for adherent cell types, hold great potential. However, the microstructures composing these arrays are fabricated from an epoxy photoresist 1002F, a growth surface unsuitable for many cell types. Optimization of the microstructures’ surface properties was conducted for the culture of satellite cells, primary muscle cells for which improved cell isolation techniques are desired. A variety of surface materials were screened for satellite cell adhesion and proliferation and compared to their optimal substrate, gelatin-coated Petri dishes. A 1-μm thick, polystyrene copolymer was applied to the microstructures by contact printing. A negatively charged copolymer of 5% acrylic acid in 95% styrene was found to be equivalent to the control Petri dishes for cell adhesion and proliferation. Cells cultured on control dishes and optimal copolymer-coated surfaces maintained an undifferentiated state and showed similar mRNA expression for two genes indicative of cell differentiation during a standard differentiation protocol. Experiments using additional contact-printed layers of extracellular matrix proteins collagen and gelatin showed no further improvements. This micropallet coating strategy is readily adaptable to optimize the array surface for other types of primary cells.}, number={3}, journal={ANALYTICAL AND BIOANALYTICAL CHEMISTRY}, author={Detwiler, David A. and Dobes, Nicholas C. and Sims, Christopher E. and Kornegay, Joe N. and Allbritton, Nancy L.}, year={2012}, month={Jan}, pages={1083–1091} }
 @article{olby_sharp_nghiem_keene_defrancesco_sidley_kornegay_schatzberg_2011, title={Clinical progression of X-linked muscular dystrophy in two German Shorthaired Pointers}, volume={238}, number={2}, journal={Journal of the American Veterinary Medical Association}, author={Olby, N. J. and Sharp, N. J. H. and Nghiem, P. P. and Keene, B. W. and DeFrancesco, T. C. and Sidley, J. A. and Kornegay, J. N. and Schatzberg, S. J.}, year={2011}, pages={207–212} }
 @article{sharp_davis_guy_cullen_steingold_kornegay_1999, title={Hydranencephaly and cerebellar hypoplasia in two kittens attributed to intrauterine parvovirus infection}, volume={121}, ISSN={["0021-9975"]}, DOI={10.1053/jcpa.1998.0298}, abstractNote={Six weeks after vaccination with modified live feline parvovirus vaccine, a cat gave birth to five kittens, three of which died soon afterwards. The remaining two kittens (A and B) survived, but at 8 weeks of age were unable to walk and showed abnormal behaviour, with lack of menace and oculovestibular responses, and severe dysmetria. These signs suggested multifocal disease associated with the cerebrum and cerebellum. Magnetic resonance imaging demonstrated severe bilateral (kitten A) or unilateral (kitten B) hydrocephalus or hydranencephaly, combined with cerebellar agenesis (kitten A) or severe hypoplasia (kitten B). Hydranencephaly was confirmed histopathologically in both kittens. Parvovirus was isolated from the kidney of one kitten. Parvoviral DNA was amplified by the polymerase chain reaction (PCR) from paraffin wax-embedded brain of both kittens. The severe malformations observed in these kittens presumably resulted from an in-utero parvovirus infection, possibly due to vaccination, that occurred late in the first, or early in the second, trimester of pregnancy.}, number={1}, journal={JOURNAL OF COMPARATIVE PATHOLOGY}, author={Sharp, NJH and Davis, BJ and Guy, JS and Cullen, JM and Steingold, SF and Kornegay, JN}, year={1999}, month={Jul}, pages={39–53} }
 @article{schatzberg_olby_breen_anderson_langford_dickens_wilton_zeiss_binns_kornegay_et al._1999, title={Molecular analysis of a spontaneous dystrophin 'knockout' dog}, volume={9}, ISSN={["0960-8966"]}, DOI={10.1016/S0960-8966(99)00011-5}, abstractNote={We have determined the molecular basis for skeletal myopathy and dilated cardiomyopathy in two male German short-haired pointer (GSHP) littermates. Analysis of skeletal muscle demonstrated a complete absence of dystrophin on Western blot analysis. PCR analysis of genomic DNA revealed a deletion encompassing the entire dystrophin gene. Molecular cytogenetic analysis of lymphocytes from the dam and both dystrophic pups confirmed a visible deletion in the p21 region of the affected canine X chromosome. Utrophin is up-regulated in the skeletal muscle, but does not appear to ameliorate the dystrophic canine phenotype. This new canine model should further our understanding of the physiological and biochemical processes in Duchenne muscular dystrophy.}, number={5}, journal={NEUROMUSCULAR DISORDERS}, author={Schatzberg, SJ and Olby, NJ and Breen, M and Anderson, LVB and Langford, CF and Dickens, HF and Wilton, SD and Zeiss, CJ and Binns, MM and Kornegay, JN and et al.}, year={1999}, month={Jul}, pages={289–295} }
 @article{thrall_larue_powers_page_johnson_george_kornegay_mcentee_levesque_smith_et al._1999, title={Use of whole body hyperthermia as a method to heat inaccessible tumours uniformly: a phase III trial in canine brain masses}, volume={15}, number={5}, journal={International Journal of Hyperthermia}, author={Thrall, D. E. and Larue, S. M. and Powers, B. E. and Page, R. L. and Johnson, J. and George, S. L. and Kornegay, J. N. and McEntee, M. C. and Levesque, D. C. and Smith, M. and et al.}, year={1999}, pages={383–398} }
 @article{hardie_ramirez_clary_kornegay_correa_feimster_robertson_1998, title={Abnormalities of the thoracic bellows: Stress fractures of the ribs and hiatal hernia}, volume={12}, ISSN={["1939-1676"]}, DOI={10.1111/j.1939-1676.1998.tb02123.x}, abstractNote={The thoracic bellows mechanism consists of the rib cage and the diaphragm. The purpose of this study was to determine if nontraumatically acquired lesions of the bellows were secondary to underlying disease. Abnormalities of the bellows, specifically stress fractures of the ribs and hiatal hernia, were found in 21 dogs and cats with underlying cardiopulmonary disease, neuromuscular disease, or metabolic disease. A case-control study of Bulldogs demonstrated that hiatal hernia was associated with the more severe manifestations of brachycephalic syndrome. Stress fractures occurred mostly in females and in cats, and involved multiple ribs. Fractures were usually related to severe respiratory effort, but also occurred in association with metabolic disease. Hiatal hernia was also associated with severe respiratory effort, but may be exacerbated if a neuromuscular disorder affecting the diaphragm is present. Abnormalities of the thoracic bellows, such as rib stress fractures and hiatal hernia, may be signs of underlying disease, rather than being primary causes of disease.}, number={4}, journal={JOURNAL OF VETERINARY INTERNAL MEDICINE}, author={Hardie, EM and Ramirez, O and Clary, EM and Kornegay, JN and Correa, MT and Feimster, RA and Robertson, ER}, year={1998}, pages={279–287} }
 @article{schatzberg_anderson_wilton_kornegay_mann_solomon_sharp_1998, title={Alternative dystrophin gene transcripts in golden retriever muscular dystrophy}, volume={21}, number={8}, journal={Muscle and Nerve}, author={Schatzberg, S. J. and Anderson, L. V. B. and Wilton, S. D. and Kornegay, J. N. and Mann, C. J. and Solomon, G. G. and Sharp, N. J. H.}, year={1998}, pages={991–998} }
 @article{kornegay_sharp_bogan_vancamp_metcalf_schueler_1994, title={CONTRACTION TENSION AND KINETICS OF THE PERONEUS LONGUS MUSCLE IN GOLDEN RETRIEVER MUSCULAR-DYSTROPHY}, volume={123}, ISSN={["1878-5883"]}, DOI={10.1016/0022-510X(94)90210-0}, abstractNote={Contraction tension and kinetics of the peroneus longus muscle were studied in dogs with the Duchenne homologue, golden retriever muscular dystrophy (GRMD), in advance of evaluating localized therapies such as myoblast transplantation. Absolute and both muscle- and body-weight-corrected twitch tension in GRMD dogs were low compared to normal litter mates at 3 months of age (p < 0.0005 for all). Tetanic tension was affected similarly. However, whereas absolute values were still reduced at 6 months (p < 0.0005 for twitch and 0.005 for tetany), twitch and tetanic tension corrected for either muscle or body weight was not statistically different, suggesting that the peroneus longus may be relatively spared in GRMD. Post-tetanic potentiation was more pronounced in GRMD versus normal dogs at both 3 (p < 0.0001) and 6 (p < 0.01) months. The degree of positive staircase at 3 months of age did not differ. Twitch contraction and relaxation times were dramatically prolonged, and there was concomitant sustained electrical activity, at, or before, 6 months of age in some severely affected dogs. Relatively few carriers were evaluated at these ages, but their values were similar to those of normal dogs. Apparent sparing of the peroneus longus muscle may limit application of this technique to evaluation of therapies administered early in life or in combination with toxins. Treatment to alter changes in contraction kinetics could also be assessed.}, number={1-2}, journal={JOURNAL OF THE NEUROLOGICAL SCIENCES}, author={KORNEGAY, JN and SHARP, NJH and BOGAN, DJ and VANCAMP, SD and METCALF, JR and SCHUELER, RO}, year={1994}, month={May}, pages={100–107} }
 @article{kornegay_sharp_schueler_betts_1994, title={Tarsal joint contracture in dogs with golden retriever muscular dystrophy}, volume={44}, number={4}, journal={Laboratory Animal Science}, author={Kornegay, J. N. and Sharp, N. J. H. and Schueler, R. O. and Betts, C. W.}, year={1994}, pages={331} }
 @article{kornegay_1991, title={Feline neurology}, volume={3}, number={3}, journal={Problems in Veterinary Medicine}, author={Kornegay, J. N.}, year={1991}, pages={309} }
 @article{kornegay_1990, title={Ataxia of the head and limbs: Cerebellar diseases in dogs and cats}, volume={1}, number={3}, journal={Progress in Veterinary Neurology}, author={Kornegay, J. N.}, year={1990}, pages={255} }
 @article{kornegay_1990, title={Imaging brain neoplasms: Computed tomography and magnetic resonance imaging}, volume={2}, number={4}, journal={Veterinary Medicine Report}, author={Kornegay, J. N.}, year={1990}, pages={372} }