Jamie Nosbisch

MeSH headings : Animals; Carrier Proteins / metabolism; Isoenzymes / metabolism; Kinetics; Mammals / metabolism; Phospholipase C gamma / genetics; Phospholipase C gamma / metabolism; Phosphorylation; Protein-Tyrosine Kinases / metabolism; Type C Phospholipases / metabolism; src Homology Domains / genetics

TL;DR: A structure-based kinetic model of PLC-γ1 is introduced, considering interactions of its regulatory Src homology 2 (SH2) domains and perturbation of those dynamics upon phosphorylation of Tyr783, a hallmark of activation. (via Semantic Scholar)

MeSH headings : Animals; Chemotaxis / physiology; Diglycerides / metabolism; Fibroblasts / metabolism; Humans; Intracellular Signaling Peptides and Proteins / metabolism; Membrane Proteins / metabolism; Models, Theoretical; Myristoylated Alanine-Rich C Kinase Substrate / metabolism; Phosphatidic Acids / metabolism; Phosphatidic Acids / physiology; Phospholipase D / metabolism; Phosphorylation; Platelet-Derived Growth Factor / metabolism; Protein Kinase C / metabolism; Protein Kinase C / physiology; Signal Transduction / physiology; Type C Phospholipases / metabolism; Type C Phospholipases / physiology

TL;DR: In simulations of wound invasion, efficient collective migration is achieved with thresholds for chemotaxis matching those of polarization in the reaction-diffusion models, and increasing DAG kinase activity can enhance the robustness of DAG/active PKC polarization with respect to chemoattractant concentration while decreasing their whole-cell levels. (via Semantic Scholar)