Joseph Milone

College of Agriculture and Life Sciences

Works (6)

Updated: July 5th, 2023 14:46

2022 article

Drone honey bees are disproportionately sensitive to abiotic stressors despite expressing high levels of stress response proteins

McAfee, A., Metz, B. N., Milone, J. P., Foster, L. J., & Tarpy, D. R. (2022, February 17). Communications Biology, Vol. 2.

By: A. McAfee n, B. Metz n, J. Milone n, L. Foster * & D. Tarpy n 

MeSH headings : Animals; Bees / drug effects; Bees / physiology; Cold Temperature; Female; Gene Expression Regulation / drug effects; Male; Neonicotinoids / toxicity; Nitro Compounds / toxicity; Pesticides / toxicity; Sex Factors; Stress, Physiological
topics (OpenAlex): Insect and Arachnid Ecology and Behavior; Insect and Pesticide Research; Plant and animal studies
TL;DR: Surprisingly, although drones are more likely to die from some stressors than workers, they exhibit higher baseline stress response proteins, suggesting that drones’ stress tolerance systems are fundamentally rewired relative to workers, and susceptibility to stress depends on more than simply gene dose or allelic diversity. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 21, 2022

2021 article

Honey bee queen health is unaffected by contact exposure to pesticides commonly found in beeswax

McAfee, A., Milone, J. P., Metz, B., McDermott, E., Foster, L. J., & Tarpy, D. R. (2021, July 26). Scientific Reports, Vol. 7.

By: A. McAfee n, J. Milone n, B. Metz n, E. McDermott n, L. Foster * & D. Tarpy n 

MeSH headings : Animals; Beekeeping; Bees / drug effects; Bees / physiology; Dose-Response Relationship, Drug; Environmental Exposure / adverse effects; Environmental Exposure / analysis; Fat Body / drug effects; Fat Body / metabolism; Female; Insect Proteins / drug effects; Insect Proteins / metabolism; Male; Oviposition / drug effects; Pesticide Residues / analysis; Pesticide Residues / toxicity; Pesticides / analysis; Pesticides / toxicity; Proteomics; Reproduction / drug effects; Sperm Count; Waxes / chemistry; Waxes / toxicity
topics (OpenAlex): Insect and Pesticide Research; Plant and animal studies; Insect and Arachnid Ecology and Behavior
TL;DR: It is suggested that previously reported associations between high levels of pesticide residues in wax and queen failure are most likely driven by indirect effects of worker exposure (either through wax or other hive products) on queen care or queen perception. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 16, 2021

2020 article

Candidate stress biomarkers for queen failure diagnostics

McAfee, A., Milone, J., Chapman, A., Foster, L. J., Pettis, J. S., & Tarpy, D. R. (2020, August 20). BMC Genomics, Vol. 21.

By: A. McAfee n, J. Milone n, A. Chapman *, L. Foster *, J. Pettis * & D. Tarpy n 

author keywords: Honey bees; Queens; Sperm viability; Biomarkers; Proteomics; Stressors; Spermatheca
MeSH headings : Bees; Biomarkers; Pesticides
topics (OpenAlex): Insect and Pesticide Research; Insect and Arachnid Ecology and Behavior; Bee Products Chemical Analysis
TL;DR: It was found that heat-shocking queens for upwards of 1 h at 40 °C was necessary to induce significant changes in the two strongest candidate heat-shock markers, and that relative humidity significantly influenced the degree of activation. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 21, 2020

2020 article

Colony-level pesticide exposure affects honey bee (Apis mellifera L.) royal jelly production and nutritional composition

Milone, J. P., Chakrabarti, P., Sagili, R. R., & Tarpy, D. R. (2020, August 29). Chemosphere, Vol. 263.

By: J. Milone n, P. Chakrabarti *, R. Sagili * & D. Tarpy n 

author keywords: Queen developmental nutrition; Pesticides; Royal jelly; Proteins; Phytosterols; Metabolites
MeSH headings : Animals; Bees; Fatty Acids; Larva; Pesticides; Pollen
topics (OpenAlex): Insect and Pesticide Research; Plant and animal studies; Bee Products Chemical Analysis
TL;DR: Pesticide treatment had a greater impact on royal jelly nutritional composition than the weight of royal jelly provisioned per queen cell, highlighting the indirect effects of pesticide exposure on queen developmental nutrition and allude to social consequences of nurse bee glandular degeneration. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 19, 2021

2020 article

Differences in larval pesticide tolerance and esterase activity across honey bee (Apis mellifera) stocks

Milone, J. P., Rinkevich, F. D., McAfee, A., Foster, L. J., & Tarpy, D. R. (2020, September 2). Ecotoxicology and Environmental Safety, Vol. 206.

By: J. Milone n, F. Rinkevich *, A. McAfee n, L. Foster * & D. Tarpy n 

author keywords: Developmental exposure; Pesticide tolerance; Honey bee; Breeding; Esterases
MeSH headings : Adaptation, Physiological / drug effects; Animals; Bees / drug effects; Bees / enzymology; Environmental Monitoring / methods; Esterases / metabolism; Larva / drug effects; Larva / enzymology; North America; Pesticides / toxicity; Pollination
topics (OpenAlex): Insect and Pesticide Research; Insect and Arachnid Ecology and Behavior; Plant and animal studies
TL;DR: It is suggested that selective breeding may inadvertently increase honey bees' sensitivity to pesticides, whereas unselected, putatively feral and Old World stocks have larvae that are more tolerant. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 16, 2020

2020 article

Vulnerability of honey bee queens to heat-induced loss of fertility

McAfee, A., Chapman, A., Higo, H., Underwood, R., Milone, J., Foster, L. J., … Pettis, J. S. (2020, March 23). Nature Sustainability.

By: A. McAfee n, A. Chapman *, H. Higo *, R. Underwood *, J. Milone n, L. Foster *, M. Guarna *, D. Tarpy n , J. Pettis *

topics (OpenAlex): Insect and Pesticide Research; Insect and Arachnid Ecology and Behavior; Plant and animal studies
TL;DR: It is found that queens have two potential routes of temperature-stress exposure: within colonies and during routine shipping, and data suggest that temperatures of 15–38 °C are safe for queens at a tolerance threshold of 11.5%, which is the viability difference associated with queen failure in the field. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 14, 2020

Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.

Certain data included herein are derived from the Web of Science© and InCites© (2026) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.