Jack P. Wang is an Assistant Professor in the Forest Biotechnology Group, Department of Forestry and Environmental Resources, College of Natural Resources at North Carolina State University. Jack received his B.S. degree in Biology and Chemistry from Waikato University, New Zealand in 2006, and completed his Ph.D. at North Carolina State University in 2012, under the mentorship of Dr. Vincent Chiang and Dr. Ron Sederoff. Jack is a forestry scientist with research experience in molecular genetics and biotechnology, particularly the integrative genomic and genetic analysis of plant metabolism for tree growth, development, and defense against biotic and abiotic stresses. Some highlights of his recent research include an unprecedented quantitative systematic analysis of the response to genetic regulation in the model tree species (Populus trichocarpa), with important immediate implications for plant growth and adaptation, as well as biofuels and biomaterials (published in 2018 in Nature Communications); demonstrating that protein phosphorylation acts as an on/off switch for AldOMT, a central enzyme in monolignol biosynthesis (published in 2015 in PNAS); characterizing the hetero-tetrameric 4CL enzyme complex formation, regulation and numerical modeling (published in 2014 in Plant Cell); and establishing a proteomic-based predictive kinetic metabolic-flux (PKMF) model of the monolignol biosynthetic pathway (published in 2014 in Plant Cell). This PKMF model predicts how monolignol pathway enzymes affect lignin content and composition, explains a long-standing paradox regarding the regulation of monolignol subunit ratios in lignin, and reveals novel mechanisms involved in the regulation of lignin biosynthesis.

Works (31)

2019 journal article

A novel synthetic-genetic-array-based yeast one-hybrid system for high discovery rate and short processing time

GENOME RESEARCH, 29(8), 1343–1351.

By: C. Yeh, Z. Wang, F. Miao, H. Ma, C. Kao, T. Hsu, J. Yu, E. Hung ...

Source: Web Of Science
Added: September 16, 2019

2019 journal article

CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

NEW PHYTOLOGIST, 222(1), 244–260.

By: X. Yan, J. Liu, H. Kim, B. Liu, X. Huang, Z. Yang, Y. Lin, H. Chen ...

Sources: Web Of Science, ORCID
Added: December 17, 2018

2019 journal article

Enzyme-Enzyme Interactions in Monolignol Biosynthesis

FRONTIERS IN PLANT SCIENCE, 9.

By: J. Wang, B. Liu, Y. Sun, V. Chiang & R. Sederoff

Sources: NC State University Libraries, ORCID
Added: January 28, 2019

2019 journal article

Flux modeling for monolignol biosynthesis

By: J. Wang

Source: ORCID
Added: April 24, 2019

2019 journal article

Hierarchical Transcription Factor and Chromatin Binding Network for Wood Formation in Populus trichocarpa

The Plant Cell, 31(3), 602–626.

By: H. Chen, J. Wang, H. Liu, H. Li, Y. Lin, R. Shi, C. Yang, J. Gao ...

Source: ORCID
Added: April 18, 2019

2019 article

Modeling cross-regulatory influences on monolignol transcripts and proteins under single and combinatorial gene knockdowns in Populus trichocarpa

Matthews, M. L., Wang, J. P., Sederoff, R., Chiang, V. L., & Williams, C. M. (2019, June 19). Cold Spring Harbor Laboratory.

By: M. Matthews, J. Wang, R. Sederoff, V. Chiang & C. Williams

Source: ORCID
Added: June 20, 2019

2019 journal article

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

NATURE COMMUNICATIONS, 10.

By: C. Straub, P. Khatibi, J. Wang, J. Conway, A. Williams-Rhaesa, I. Peszlen, V. Chiang, M. Adams, R. Kelly

Source: Web Of Science
Added: August 26, 2019

2019 review

Systems and Synthetic Biology of Forest Trees: A Bioengineering Paradigm for Woody Biomass Feedstocks

FRONTIERS IN PLANT SCIENCE.

By: A. Myburg, S. Hussey, J. Wang, N. Street & E. Mizrachi

Source: Web Of Science
Added: July 8, 2019

2019 journal article

The AREB1 Transcription Factor Influences Histone Acetylation to Regulate Drought Responses and Tolerance in Populus trichocarpa

By: J. Wang

Source: ORCID
Added: April 24, 2019

2018 journal article

Assessing the impact of the 4CL enzyme complex on the robustness of monolignol biosynthesis using metabolic pathway analysis

PLoS ONE, 13(3).

By: P. Naik, J. Wang, R. Sederoff, V. Chiang, C. Williams & J. Ducoste

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2018 journal article

Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis.

Nature Communications, 4.

By: J. Wang, M. Matthews, C. Williams, R. Shi, C. Yang, S. Tunlaya-Anukit, H. Chen, Q. Li ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa.

Proceedings of the National Academy of Sciences of the United States of America, 11(45), E9722–9729.

By: Y. Lin, H. Chen, Q. Li, W. Li, J. Wang, R. Shi, S. Tunlaya-Anukit, P. Shuai ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Tissue and cell-type co-expression networks of transcription factors and wood component genes in Populus trichocarpa

Planta, 245(5), 927–938.

By: R. Shi, J. Wang, Y. Lin, Q. Li, Y. Sun, H. Chen, R. Sederoff, V. Chiang

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2016 book

A Proteomic-Based Quantitative Analysis of the Relationship Between Monolignol Biosynthetic Protein Abundance and Lignin Content Using Transgenic Populus trichocarpa

Recent Advances in Polyphenol Research (Vol. 5, pp. 89–107). Chichester, West Sussex, England: Wiley-Blackwell.

By: J. Wang, S. Tunlaya-Anukit, R. Shi, T. Yeh, L. Chuang, F. Isik, C. Yang, J. Liu ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2016 journal article

A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa

Tree Genetics and Genomes, 12(2).

By: C. Lin, Q. Li, S. Tunlaya-Anukit, R. Shi, Y. Sun, J. Wang, J. Liu, P. Loziuk ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

4-Coumaroyl and caffeoyl shikimic acids inhibit 4-coumaric acid:coenzyme a ligases and modulate metabolic flux for 3-hydroxylation in monolignol biosynthesis of populus trichocarpa

Molecular Plant, 8(1), 176–187.

By: C. Lin, J. Wang, Q. Li, H. Chen, J. Liu, P. Loziuk, J. Song, C. Williams ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Comparative proteomic analysis of Populus trichocarpa early stem from primary to secondary growth

Journal of Proteomics, 126, 94–108.

By: J. Liu, G. Hai, C. Wang, S. Cao, W. Xu, Z. Jia, C. Yang, J. Wang, S. Dai, Y. Cheng

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Elucidation of Xylem-Specific Transcription Factors and Absolute Quantification of Enzymes Regulating Cellulose Biosynthesis in Populus trichocarpa

Journal of Proteome Research, 14(10), 4158–4168.

By: P. Loziuk, J. Parker, W. Li, C. Lin, J. Wang, Q. Li, R. Sederoff, V. Chiang, D. Muddiman

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis

Proceedings of the National Academy of Sciences of the United States of America, 112(27), 8481–8486.

By: J. Wang, L. Chuang, P. Loziuk, H. Chen, Y. Lin, R. Shi, G. Qu, D. Muddiman, R. Sederoff, V. Chiang

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2014 journal article

A simple improved-throughput xylem protoplast system for studying wood formation

Nature Protocols, 9(9), 2194–2205.

By: Y. Lin, W. Li, H. Chen, Q. Li, Y. Sun, R. Shi, C. Lin, J. Wang ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2014 journal article

Complete proteomic-based enzyme reaction and inhibition kinetics reveal how monolignol biosynthetic enzyme families affect metabolic flux and lignin in Populus trichocarpa

Plant Cell, 26(3), 894–914.

By: J. Wang, P. Naik, H. Chen, R. Shi, C. Lin, J. Liu, C. Shuford, Q. Li ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2014 journal article

Plant biotechnology for lignocellulosic biofuel production

Plant Biotechnology Journal, 12(9), 1174–1192.

By: Q. Li, J. Song, S. Peng, J. Wang, G. Qu, R. Sederoff, V. Chiang

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2014 journal article

Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric Acid:Coenzyme A Ligase Protein Complex Formation, Regulation, and Numerical Modeling

The Plant Cell, 3(3), 876–893.

By: H. Chen, J. Song, J. Wang, Y. Lin, J. Ducoste, C. Shuford, J. Liu, Q. Li ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Monolignol pathway 4-coumaric acid: Coenzyme A ligases in populus trichocarpa: Novel specificity, metabolic regulation, and simulation of coenzyme a ligation fluxes

Plant Physiology, 161(3), 1501–1516.

By: H. Chen, J. Song, C. Williams, C. Shuford, J. Liu, J. Wang, Q. Li, R. Shi ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Regulation of phenylalanine ammonia-lyase (PAL) gene family in wood forming tissue of Populus trichocarpa

Planta, 238(3), 487–497.

By: R. Shi, C. Shuford, J. Wang, Y. Sun, Z. Yang, H. Chen, S. Tunlaya-Anukit, Q. Li ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Understanding the role of proteolytic digestion on discovery and targeted proteomic measurements using liquid chromatography tandem mass spectrometry and design of experiments

Journal of Proteome Research, 12(12), 5820–5829.

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2012 journal article

Comprehensive quantification of monolignol-pathway enzymes in populus trichocarpa by protein cleavage isotope dilution mass spectrometry

Journal of Proteome Research, 11(6), 3390–3404.

By: C. Shuford, Q. Li, Y. Sun, H. Chen, J. Wang, R. Shi, R. Sederoff, V. Chiang, D. Muddiman

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2012 journal article

Functional redundancy of the two 5-hydroxylases in monolignol biosynthesis of Populus trichocarpa: LC–MS/MS based protein quantification and metabolic flux analysis

Planta, 236(3), 795–808.

By: J. Wang, C. Shuford, Q. Li, J. Song, Y. Lin, Y. Sun, H. Chen, C. Williams ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

Down-regulation of glycosyltransferase 8D genes in Populus trichocarpa caused reduced mechanical strength and xylan content in wood

Tree Physiology, 31(2), 226–236.

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2007 journal article

A novel O-methyl transferase-like gene with a drastic ectopic expression in response to tension wood formation in Populus trichocarpa

Cellulose Chemistry and Technology, 41(9-10), 521–528.

By: T. Yeh, J. Wang, R. Shi, Y. Sun & V. Chiang

Source: ORCID
Added: December 17, 2018

2007 journal article

A novel O-methyl transferase-like gene with drastic ectopic expression in response to tension wood formation in Populus trichocarpa

Cellulose Chemistry and Technology, 41, 521–528.

By: W. Yeh T.F., S. Shi R. & C. V.L.

Source: NC State University Libraries
Added: August 6, 2018

Employment

2018 - present

North Carolina State University Raleigh, NC, US
Assistant Professor Forest Biotechnology Group

Education

2007 - 2012

North Carolina State University Raleigh, NC, US
Ph.D. Forestry and Environmental Resources