Works (5)

Updated: July 5th, 2023 15:35

2022 journal article

The Stickland Reaction Precursor trans-4-Hydroxy-l-Proline Differentially Impacts the Metabolism of Clostridioides difficile and Commensal Clostridia

MSPHERE, 7(2).

By: A. Reed n, J. Fletcher n, Y. Huang*, R. Thanissery n, A. Rivera n, R. Parsons n, A. Stewart n, D. Kountz* ...

author keywords: Clostridioides difficile; hydroxyproline; amino acids; Clostridia; Stickland reaction; colonization resistance; Stickland fermentation
MeSH headings : Animals; Clostridioides; Clostridioides difficile / genetics; Clostridium; Clostridium Infections / metabolism; Hydroxyproline / chemistry; Hydroxyproline / metabolism; Mice; Peptide Hydrolases; Proline / metabolism
TL;DR: A C. difficile ΔhypD mutant mutant was constructed and found that it was modestly impaired in fitness in a mouse model of infection, and was associated with an altered microbiota when compared to mice challenged with the wild-type strain. (via Semantic Scholar)
Source: Web Of Science
Added: July 5, 2022

2021 journal article

Clostridioides difficile exploits toxin-mediated inflammation to alter the host nutritional landscape and exclude competitors from the gut microbiota


MeSH headings : Animals; Anti-Bacterial Agents / adverse effects; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Bacterial Toxins / genetics; Bacterial Toxins / immunology; Bacterial Toxins / metabolism; Bacteroides / drug effects; Bacteroides / metabolism; Clostridioides difficile / genetics; Clostridioides difficile / immunology; Clostridioides difficile / metabolism; Clostridium Infections / immunology; Clostridium Infections / microbiology; Clostridium Infections / pathology; Disease Models, Animal; Extracellular Matrix / metabolism; Female; Gastrointestinal Microbiome / drug effects; Gastrointestinal Microbiome / immunology; Gene Expression Regulation, Bacterial / immunology; Host-Pathogen Interactions / genetics; Host-Pathogen Interactions / immunology; Humans; Intestinal Mucosa / immunology; Intestinal Mucosa / microbiology; Intestinal Mucosa / pathology; Male; Matrix Metalloproteinases / metabolism; Mice; Nutrients / metabolism; Proteolysis; RNA, Bacterial / genetics; RNA, Bacterial / isolation & purification; RNA-Seq; Sigma Factor / genetics; Sigma Factor / immunology; Sigma Factor / metabolism; Transcriptome / immunology
TL;DR: Insight is provided into how toxin-induced inflammation alters C. difficile metabolism, host tissue gene expression and gut microbiota, together influencing a beneficial niche for infection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 20, 2021

2019 article

Human fecal metabolomic profiling could inform Clostridioides difficile infection diagnosis and treatment

Theriot, C. M., & Fletcher, J. R. (2019, September 3). JOURNAL OF CLINICAL INVESTIGATION, Vol. 129, pp. 3539–3541.

By: C. Theriot n & J. Fletcher n

MeSH headings : Clostridioides difficile; Clostridium Infections; Diarrhea; Feces; Humans; Microbiota
TL;DR: A logistic regression model based on the fecal metabolome is used and a metabolic definition of human C. difficile infection is constructed, which could improve diagnostic accuracy and aid in the development of targeted therapeutics against this pathogen. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: September 30, 2019

2018 journal article

Bacterial lipoproteins and other factors released by Francisella tularensis modulate human neutrophil lifespan: Effects of a TLR1 SNP on apoptosis inhibition


By: L. Kinkead*, L. Whitmore*, J. McCracken*, J. Fletcher*, B. Ketelsen*, J. Kaufman*, B. Jones*, D. Weiss*, J. Barker*, L. Allen*

author keywords: apoptosis; lipoproteins; neutrophils; SNP; TLR2; tularaemia
MeSH headings : Apoptosis / physiology; Bacterial Proteins / metabolism; Francisella tularensis / genetics; Francisella tularensis / metabolism; Humans; Lipoproteins / metabolism; Macrophages / metabolism; Macrophages / microbiology; Macrophages / physiology; Neutrophils / metabolism; Neutrophils / microbiology; Neutrophils / physiology; Polymorphism, Single Nucleotide / genetics; Toll-Like Receptor 1 / genetics; Tularemia / metabolism; Tularemia / microbiology; Virulence / genetics; Virulence Factors / metabolism
TL;DR: It is demonstrated that bacterial conditioned medium (CM) can delay apoptosis in the absence of direct infection, and bacterial lipoproteins (BLPs) are identified as active factors in CM. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2018 journal article

Shifts in the Gut Metabolome and Clostridium difficile Transcriptome throughout Colonization and Infection in a Mouse Model

MSPHERE, 3(2).

By: J. Fletcher n, S. Erwin n, C. Lanzas n & C. Theriot n

Contributors: J. Fletcher n, S. Erwin n, C. Lanzas n & C. Theriot n

author keywords: Clostridium difficile; amino acids; intestinal colonization; metabolomics; peptides; transcriptomics
MeSH headings : Amino Acids, Branched-Chain / metabolism; Animals; Anti-Bacterial Agents / administration & dosage; Carbohydrate Metabolism; Cecum / microbiology; Clostridioides difficile / metabolism; Clostridium Infections / metabolism; Clostridium Infections / microbiology; Female; Gastrointestinal Microbiome; Gene Expression Profiling; Male; Mass Spectrometry; Metabolome; Metabolomics; Mice; Mice, Inbred C57BL; Peptide Hydrolases / genetics; Proline / metabolism; Sequence Analysis, RNA; Transcriptome
TL;DR: The gut environment after antibiotics and during the initial stages of C. difficile colonization and infection is defined and amino acids, in particular, proline and branched-chain amino acids and carbohydrates decrease in abundance over time and that C.difficile gene expression is consistent with their utilization in vivo. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

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