@article{olivry_paps_amalric_2021, title={Transient and reversible reduction of stratum corneum filaggrin degradation products after allergen challenge in experimentally mite-sensitised atopic dogs}, volume={33}, ISSN={["1365-3164"]}, url={https://doi.org/10.1111/vde.13026}, DOI={10.1111/vde.13026}, abstractNote={A defective skin barrier occurs in dogs with atopic dermatitis, and there is controversy over whether this defect pre-exists, or is secondary to allergic inflammation.To study if an allergen challenge decreases the natural moisturising factor (NMF), which contains the main filaggrin degradation products.Four house dust mite (HDM)-sensitised adult atopic dogs from a research colony.Dogs were challenged epicutaneously with HDMs on the right lateral abdomen while the left thorax served as control. We swabbed the skin surface before, and at days (D)1, D2, D3, D7 and D28 after challenge, on both selected sites; swabs were soaked in detergent and frozen until assayed. The NMF components were measured by liquid chromatography-tandem mass spectrometry (LC/MS-MS).The allergen challenge induced moderate skin lesions at the application sites, and also mild erythema at the control areas. The allergen provocation led to significant decreases in the total NMF and its components trans-urocanic acid (t-UCA), pyrrolidone carboxylic acid (PCA) and serine on both sites. Lesion scores abated by D7 and the NMF concentrations had re-increased by D28. Skin lesion scores correlated negatively with the total NMF, t-UCA and PCA concentrations.In this experimental model, a single epicutaneous allergen challenge led to a transient and reversible decrease in skin surface NMF and its components, and concentrations were negatively correlated with skin lesion scores. These observations suggest that some of the skin barrier anomalies seen in atopic dogs likely develop secondarily to the underlying cutaneous allergic inflammation.Un défaut de barrière cutanée existe chez le chien atopique et il y a controverse si ce défaut préexiste ou s’il est secondaire à une inflammation allergique.Etudier si une provocation allergénique diminue le NMF (natural moisturising factor) qui contient les principaux produits de dégradation de la filaggrine.Quatre chiens atopiques sensibilisés aux acariens de poussière de maison (HDM) d’une colonie de recherche. MÉTHODES: Les chiens ont été provoqués par test épicutanés avec HDMs sur l’abdomen latéral droit pendant que le thorax gauche servait de contrôle. Nous avons prélevés la surface de la peau avant et après provocation à D1, D2, D3, D7 et D28 sur les deux faces sélectionnées; les écouvillons ont été trempés dans un détergent et congelés jusqu’aux tests. Les composés NMF ont été mesurés par LC/MS-MS (liquid chromatography-tandem mass spectrometry). RÉSULTATS: La provocation allergénique a induit des lésions modérées aux sites d’application, et aussi un érythème modéré aux zones contrôles. Le test de provocation à mené à des diminutions significatives du NMF total et ses composés t-UCA (trans-urocanic acid), PCA (pyrrolidone carboxylic acid) et sérine sur les deux sites. Les scores lésionnels se calmaient à D7 et les concentrations de NMF ré-augmentaient à D28. Les scores de lésions cutanées corrélaient négativement avec les concentrations de NMF total, t-UCA et PCA.Dans ce modèle expérimental, un test allergénique epicutané unique mène à une diminution réversible et transitoire de NMF de la surface cutanée et de ses composés et les concentrations sont négativement corrélées avec les scores de lésion cutanée. Ces observations suggèrent que certaines des anomalies de barrière cutanée observées chez les chiens atopiques pourraient se développer secondairement à l’inflammation cutanée allergique sous jacente.INTRODUCCIÓN: en perros con dermatitis atópica se observa una barrera cutánea defectuosa, y existe controversia sobre si este defecto existe antes o es secundario a la inflamación alérgica. OBJETIVOS: Estudiar si una exposición con alérgenos disminuye el factor de hidratación natural (NMF), que contiene los principales productos de degradación de la filagrina. ANIMALES: cuatro perros adultos atópicos sensibilizados contra los ácaros del polvo doméstico (HDM) de una colonia de investigación. MÉTODOS: los perros fueron expuestos epicutáneamente con HDM en el abdomen lateral derecho mientras que el tórax izquierdo sirvió como control. Limpiamos la superficie de la piel antes y en los días (D) 1, D2, D3, D7 y D28 después de la exposición, en ambos sitios seleccionados; los hisopos se empaparon en detergente y se congelaron hasta su análisis. Los componentes de NMF se midieron mediante cromatografía líquida-espectrometría de masas en tándem (LC/MS-MS). RESULTADOS: la exposición al alérgeno indujo lesiones cutáneas moderadas en los lugares de aplicación y también eritema leve en las áreas de control. La provocación del alérgeno condujo a disminuciones significativas en el NMF total y en sus componentes ácido transurocánico (t-UCA), ácido pirrolidona carboxílico (PCA) y serina en ambos sitios. Las puntuaciones de las lesiones disminuyeron al D7 y las concentraciones de NMF habían vuelto a aumentar en el D28. Las puntuaciones de las lesiones cutáneas se correlacionaron negativamente con las concentraciones totales de NMF, t-UCA y PCA. CONCLUSIONES E IMPORTANCIA CLÍNICA: en este modelo experimental, una sola exposición a alérgeno epicutáneo condujo a una disminución transitoria y reversible en el NMF de la superficie de la piel y sus componentes, y las concentraciones se correlacionaron negativamente con las puntuaciones de las lesiones cutáneas. Estas observaciones sugieren que algunas de las anomalías de la barrera cutánea observadas en perros atópicos probablemente se desarrollen de forma secundaria a la inflamación alérgica cutánea subyacente.Bei Hunden mit atopischer Dermatitis besteht eine defekte Hautbarriere und es besteht eine Kontroverse darüber, ob dieser Defekt wirklich vor-existiert oder ob er sekundär durch die allergische Entzündung verursacht wird.Es sollte untersucht werden, ob eine Provokation mit dem Allergen den natürlichen Feuchthalte-Faktor (NMF), der die hauptsächlichen Abbauprodukte von Filaggrin enthält, reduziert.Vier erwachsene atopische Hunde aus einer Forschungskolonie, die mit Hausstaubmilben (HDM) sensibilisiert worden waren.Die Hunde wurden epikutan mit den HDMs rechts lateral am Abdomen provoziert, während der linke Thorax als Kontrolle diente. Wir entnahmen vorher und an den Tagen (D) 1, D2, D3, D7 und D28 nach der Provokation von beiden ausgewählten Seiten Tupferproben; die Tupfer wurden in Detergens getränkt und bis zur Untersuchung eingefroren. Die NMF Komponenten wurden mittels Hochleistungsflüssigkeitschromatografie Tandem Massenspektrometrie (LC/MS-MS) gemessen.Die Allergen Provokation induzierte moderate Hautveränderungen an den Applikationsstellen, sowie ein mildes Erythem an den Kontrollstellen. Die Allergen Provokation führte auf beiden Seiten zu signifikanten Abnahmen der totalen NMF und seiner Komponenten Trans-Urocaninsäure (t-UCA), Pyrrolidoncarbonsäure (PCA) und Serin. Die Läsionswerte nahmen mit D7 ab und die NMF Konzentrationen hatten mit D28 wieder zugenommen. Die Werte der Hautläsionen korrelierten negativ mit den Gesamt NMF, t-UCA und PVA Konzentrationen.In diesem experimentellen Modell führt eine einzige epikutane Allergenprovokation zu einer vorübergehenden und reversiblen Zunahme der NMF der Hautoberfläche und seiner Komponenten, die Konzentrationen waren mit den Hautwerten negativ korreliert. Diese Beobachtungen weisen darauf hin, dass einige der Anomalien der Hautbarriere, die bei atopischen Hunden gesehen werden, sich sekundär aufgrund der zugrundeliegenden allergischen Hautentzündung entwickeln.背景: アトピー性皮膚炎の犬では皮膚バリアの欠損が見られるが、この欠損が以前から存在していたのか、あるいはアレルギー性炎症に二次的に生じたものなのかについては議論が分かれている。 目的: 本研究の目的は、アレルゲン負荷が、主なフィラグリン分解産物を含む天然保湿因子 (NMF) を減少させるかどうかを研究することであった。 被験動物: 研究用コロニーのハウスダストマイト (HDM) 感作アトピー成犬4頭。 方法: 犬の右側腹部にHDMを付着させ、左胸部はコントロールとした。負荷前、負荷後1日目、2日目、3日目、7日目、28日目に、選択した両部位の皮膚表面を綿棒で採取し、綿棒は洗剤に浸して測定するまで凍結させた。NMFの成分は、液体クロマトグラフィー・タンデム質量解析法 (LC/MS-MS) で測定した。 結果: アレルゲン負荷は、塗布部位に中程度の皮膚病を誘発し、コントロール部位にも軽度の紅斑が見られた。アレルゲンの誘発により、両部位のNMFおよびその成分であるtrans-urocanic acid (t-UCA)、pyrrolidone carboxylic acid (PCA)、serineが有意に減少した。皮膚病変のスコアはD7までに減少し、NMFの濃度はD28までに再び上昇した。皮膚病変のスコアは、NMF、t-UCAおよびPCAの合計濃度と負の相関があった。 結論と臨床上の重要性: この実験モデルでは、アレルゲンの単回外皮投与により、皮膚表面のNMFとその成分が一過性かつ可逆的に減少し、その濃度は皮膚病変のスコアと負の相関を示した。これらの観察結果は、アトピー犬に見られる皮膚バリアの異常のいくつかは、根底にある皮膚のアレルギー性炎症に続いて発症する可能性が高いことを示唆している。.背景: 特应性皮炎犬的皮肤屏障存在缺陷, 对于该缺陷是预先存在还是继发于过敏性炎症存在争议。 目的: 研究过敏原激发是否会降低含有主要丝聚蛋白降解产物的天然保湿因子(NMF)。 动物: 来自一个研究种群的4只屋尘螨(HDM)致敏的成年特应性犬。 方法: 在犬右侧腹部用HDM进行皮外激发, 同时左胸作为对照。我们在激发前和激发后第(D)1、D2、D3、D7和D28天, 在两个选定部位擦拭皮肤表面; 将拭子浸泡在洗涤剂中并冷冻直至测定。通过液相色谱-串联质谱法(LC/MS-MS)测定NMF组分。 结果: 过敏原激发在用药部位引起中度皮肤病变, 在对照区域也引起轻度红斑。过敏原激发导致两个位点的总NMF及其组分反式尿烷酸(t-UCA)、吡咯烷酮羧酸(PCA)和丝氨酸显著降低。病变评分在D7时减轻, NMF浓度在D28时重新增加。皮肤病变评分与总NMF、t-UCA和PCA浓度呈负相关。 结论和临床重要性: 在该实验模型中, 单次表皮过敏原激发导致皮肤表面NMF及其组分一过性和可逆性降低, 且浓度与皮肤病变评分呈负相关。这些观察结果表明, 在特应性犬中观察到的一些皮肤屏障异常可能继发于潜在的皮肤过敏炎症。.Defeitos de barreira cutânea ocorrem em cães com dermatite atópica, e há controvérsias se esse defeito é pré-existente, ou secundário à inflamação alérgica.Estudar se um desafio alergênico é capaz reduzir o fator hidratante natural (NMF), que contém os principais produtos de degradação de filagrina.Quatro cães atópicos adultos sensibilizados por ácaros da poeira doméstica (HDM) de uma colônia de pesquisa. MÉTODOS: Os cães foram desafiados por via epicutânea com HDMs no abdômen lateral direito, enquanto o tórax esquerdo serviu como controle. Friccionamos a superfície da pele com swabs antes, e nos dias (D) 1, D2, D3, D7 e D28 após o desafio, em ambos os locais selecionados; os swabs permaneceram embebidos em detergente e congelados até serem processados. Os componentes NMF foram medidos por espectrometria de massa de cromatografia líquida-tandem (LC/MS-MS).O desafio alergênico induziu lesões de pele moderada nos locais de aplicação, e também eritema discreto nas áreas de controle. O desafio alergênico levou a diminuições significativas no NMF total e em seus componentes ácido trans-urocânico (t-UCA), ácido carboxílico pirrolidona (PCA) e serina em ambos os locais. Os escores de lesão diminuíram no D7 e as concentrações de NMF aumentaram novamente no D28. Os escores de lesão de pele correlacionaram negativamente com as concentrações de NMF total, t-UCA e PCA. CONCLUSÕES E IMPORTÂNCIA CLÍNICA: Neste modelo experimental, um único desafio de alergênico epicutâneo levou a uma redução transitória e reversível NMF superficial e seus componentes, e as concentrações foram negativamente correlacionadas com as pontuações da lesão da pele. Essas observações sugerem que algumas das anomalias de barreira da pele observadas em cães atópicos provavelmente se desenvolvem secundariamente à inflamação alérgica cutânea subjacente.}, number={1}, journal={VETERINARY DERMATOLOGY}, publisher={Wiley}, author={Olivry, Thierry and Paps, Judy S. and Amalric, Nicolas}, year={2021}, month={Sep} }
 @article{mishra_wheeler_pitake_ding_jiang_fukuyama_paps_ralph_coyne_parkington_et al._2020, title={Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch}, volume={31}, ISSN={["2211-1247"]}, DOI={10.1016/j.celrep.2020.03.036}, abstractNote={Chronic allergic itch is a common symptom affecting millions of people and animals, but its pathogenesis is not fully explained. Herein, we show that periostin, abundantly expressed in the skin of patients with atopic dermatitis (AD), induces itch in mice, dogs, and monkeys. We identify the integrin αVβ3 expressed on a subset of sensory neurons as the periostin receptor. Using pharmacological and genetic approaches, we inhibited the function of neuronal integrin αVβ3, which significantly reduces periostin-induced itch in mice. Furthermore, we show that the cytokine TSLP, the application of AD-causing MC903 (calcipotriol), and house dust mites all induce periostin secretion. Finally, we establish that the JAK/STAT pathway is a key regulator of periostin secretion in keratinocytes. Altogether, our results identify a TSLP-periostin reciprocal activation loop that links the skin to the spinal cord via peripheral sensory neurons, and we characterize the non-canonical functional role of an integrin in itch.}, number={1}, journal={CELL REPORTS}, author={Mishra, Santosh K. and Wheeler, Joshua J. and Pitake, Saumitra and Ding, Huiping and Jiang, Changyu and Fukuyama, Tomoki and Paps, Judy S. and Ralph, Patrick and Coyne, Jacob and Parkington, Michelle and et al.}, year={2020}, month={Apr} }
 @article{tamamoto‐mochizuki_paps_olivry_2019, title={Proactive maintenance therapy of canine atopic dermatitis with the anti‐
            IL
            ‐31 lokivetmab. Can a monoclonal antibody blocking a single cytokine prevent allergy flares?}, volume={30}, ISSN={0959-4493 1365-3164}, url={http://dx.doi.org/10.1111/vde.12715}, DOI={10.1111/vde.12715}, abstractNote={Once the signs of canine atopic dermatitis (AD) are controlled, the proactive application of topical glucocorticoids can delay disease flares. We wished to determine if the proactive administration of the anti-IL-31 lokivetmab would prevent or delay flares of canine AD. We tested this strategy in four Maltese-beagle atopic dogs before enrolling 21 dogs with spontaneous AD. In our acute AD model, house dust mite (HDM)-sensitized dogs were injected once with lokivetmab. After seven days, an HDM suspension was applied epicutaneously, and both skin lesions and pruritus manifestations were quantified for 24 h. In a second study, 21 dogs with spontaneous AD controlled with anti-allergic drugs were treated with lokivetmab per manufacturer's recommendations; all anti-allergic drugs were discontinued within four weeks after the first injection. All dogs were followed prospectively for at least one year and the time-to-flare (TTF) of AD after the last day of anti-allergic treatment was determined. In the experimental study, one injection of lokivetmab prevented nearly all expected allergen-induced pruritus manifestations but not skin lesion development. In dogs with spontaneous AD, the median TTF after lokivetmab proactive therapy was 63 days. One-fourth of dogs did not exhibit a flare for at least one year while receiving lokivetmab monotherapy. Although lokivetmab seems more effective to prevent pruritus than skin lesions in dogs with experimental AD' it also can delay disease flares in some dogs with the spontaneous disease. Studies are needed to identify those patients most likely to respond to such a proactive regimen. Lorsque les signes de dermatite atopique canine (AD) sont contrôlés, l'application proactive de corticoïdes topiques peut retarder les poussées aiguës. Nous souhaitons déterminer si l'administration proactive d'anti-IL-31, lokivetmab, peut prévenir ou retarder les poussées de AD canine. Nous testons cette stratégie sur quatre chiens atopiques beagles avant l'enrôlement de 21 chiens spontanément atopiques. Dans notre modèle d’AD aiguë, les chiens sensibilisés aux acariens de poussière de maison (HDM) ont reçu une injection de lokivetmab. Après sept jours, une suspension de HDM a été appliquée en épicutané et les lésions cutanées et le prurit ont été quantifiés pendant 24h. Dans une seconde étude, 21 chiens avec une AD spontanée contrôlée par des médicaments anti-allergiques ont été traités par du lokivetmab selon les recommandations du laboratoire; tous les anti-allergiques ont été stoppés pendant quatre semaines après la première injection. Tous les chiens ont été enrôlés prospectivement pendant au moins un an et le temps de rechute (TTF) de l’AD après le dernier jour de traitement antiallergique a été déterminé. Dans l’étude expérimentale, une injection de lokivetmab a permis la prévention de presque toutes les manifestations prurigineuses attendues induites par les allergènes mais pas le développement des lésions cutanées. Chez les chiens avec AD spontanée, le TTF médian après le lokivetmab proactif était de 63 jours. Un quart des chiens n'ont pas montré de signes de poussée de AD pendant au moins un an en recevant du lokivetmab en monothérapie. Bien que le lokivetmab semble plus efficace pour prévenir le prurit que les lésions cutanées chez les chiens présentant une AD expérimentale, il peut aussi retarder les poussées de la dermatose chez certains chiens présentant la forme spontanée. Des études sont nécessaires pour identifier les patients les plus sujets à répondre à de tels protocoles proactifs. una vez que se controlan los signos de la dermatitis atópica canina (AD), la aplicación proactiva de glucocorticoides tópicos puede retrasar los brotes de la enfermedad. determinar si la administración proactiva de lokivetmab anti-IL-31 prevendría o retrasaría los brotes de AD caninos. probamos esta estrategia en cuatro perros atópicos de mestizos Beagle-Maltés antes de inlcuir a 21 perros con AD espontánea. en nuestro modelo de AD agudo, los perros sensibilizados con ácaros del polvo doméstico (HDM) se inyectaron una vez con lokivetmab. Después de siete días, se aplicó epicutáneamente una suspensión de HDM y se cuantificaron las lesiones de la piel y las manifestaciones de prurito durante 24 h. En un segundo estudio, 21 perros con EA espontánea controlada con fármacos antialérgicos fueron tratados con lokivetmab según las recomendaciones del fabricante; todos los medicamentos antialérgicos se suspendieron cuatro semanas después de la primera inyección. Todos los perros fueron seguidos prospectivamente durante al menos un año y se determinó el tiempo hasta el brote (TTF) de AD después del último día de tratamiento antialérgico. en el estudio experimental, una inyección de lokivetmab previno casi todas las manifestaciones esperadas de prurito inducidas por alérgenos, pero no el desarrollo de lesiones en la piel. En perros con AD espontánea, la mediana de TTF después de la terapia proactiva con lokivetmab fue de 63 días. Una cuarta parte de los perros no exhibieron un brote durante al menos un año mientras recibían monoterapia con lokivetmab. aunque el lokivetmab parece ser más efectivo para prevenir el prurito que las lesiones cutáneas en perros con AD experimental, también puede retrasar los brotes de la enfermedad en algunos perros con la enfermedad espontánea. Se necesitan nuevos estudios para identificar a aquellos pacientes con mayor probabilidad de responder a un régimen proactivo de este tipo. Wenn die Zeichen einer atopischen Dermatitis (AD) des Hundes einmal kontrolliert sind, kann die proaktive Verabreichung von topischen Glukokortikoiden ein Wiederaufflammen der Erkrankung verzögern. Wir wollten feststellen, ob die proaktive Verabreichung von anti-IL-31 Lokivetmab ein Wiederaufflammen der AD des Hundes verhindern oder verzögern kann. Wir testeten diese Strategie bei vier atopischen Malteser-Beagles, bevor wir 21 Hunde mit spontaner AD in die Studie aufnahmen. In unserem akuten AD Modell wurden mit Hausstaubmilben-sensibilisierte Hunde einmal mit Lokivetmab injiziert. Nach sieben Tagen wurde eine HDM Suspension epikutan appliziert und die Hautveränderungen sowie die Manifestation des Pruritus 24h lang quantifiziert. In einer zweiten Studie wurden 21 Hunde mit spontaner AD, die mit anti-allergischen Medikamenten kontrolliert wurden, mit Lokivetmab nach den Anweisungen der Hersteller behandelt; alle anti-allergischen Medikamente wurden innerhalb von vier Wochen nach der ersten Injektion abgesetzt. Alle Hunde wurden für mindestens ein Jahr lang prospektiv nachverfolgt und die Time-To-Flare (Zeit bis zum Aufflammen; TTF) der AD nach dem letzten Tag der anti-allergischen Behandlung bestimmt. In der experimentellen Studie verhinderte eine Injektion von Lokivetmab fast jeglichen erwarteten Allergen-induzierten Pruritus, aber nicht die Entstehung von Hautveränderungen. Bei Hunden mit spontaner AD betrug die mediane TTF nach proaktiver Therapie mit Lokivetmab 63 Tage. Ein Viertel der Hunde zeigte für mindestens ein Jahr kein Aufflammen während eine Lokivetmab Monotherapie verabreicht wurde. Obwohl Lokivetmab bei experimenteller AD wirksamer sein dürfte beim Verhindern von Juckreiz als beim Verhindern von Hautveränderungen, kann es ebenso ein Aufflammen der Krankheit bei einigen Hunden mit der spontanen Erkrankung hinauszögern. Es sind Studien nötig, die Patienten identifizieren, die am wahrscheinlichsten von so einem proaktiven Behandlungsregime profitieren würden. 犬アトピー性皮膚炎(AD)の兆候はいったんコントロールされれば、外用グルココルチコイド製剤によるプロアクティブ療法が病気の再燃を遅延させることができる。 本研究の目的は、抗-IL-31製剤のlokivetmabによるプロアクティブ療法が、犬アトピー性皮膚炎の再燃を予防または遅延するかどうかを決定することである。 我々は自然発症した21頭のAD犬を入症する前に、4頭のマルチーズ-ビーグルMIXアトピー犬にこの戦略を試験した。 我々の急性ADモデルにおいて、ハウスダストマイト(HDM)感作犬にlokivetmabを1回注射した。7日後、HDM懸濁液を皮膚に塗布し、皮膚病変および掻痒症状を24時間定量した。第二の研究として、抗アレルギー薬でコントロールされた自然発症AD犬21頭を製造業者の推奨する用量用法に従いlokivetmabで治療した。全ての抗アレルギー薬は最初の注射後4週間以内に中止した。全ての犬を少なくとも1年間前向き調査し、抗アレルギー治療を適用した最終日からAD再燃までの時間(TTF)を決定した。 実験的研究では1回のlokivetmab注射は、ほぼすべての予想されるアレルゲン誘発性掻痒症状を防いだが、皮膚病変の発症は防がなかった。自然発症AD犬では、lokivetmabによるプロアクティブ療法後、TTF中央値は63日であった。4頭に1頭の犬が、lokivetmab単独療法を受けている間、少なくとも1年間再燃を示さなかった。 実験AD犬では、lokivetmabは皮膚病変より掻痒を防止するのに有効であるように思われたが、自然発症AD犬では疾患の再燃を遅延させることもできた。このようなプロアクティブ療法に最も反応を示しそうな患者を特定する研究が必要である。 Quando os sinais clínicos da dermatite atópica (DA) estão sob controle, a aplicação proativa de glicocorticoides tópicos pode espaçar as crises da doença. Nosso objetivo foi determinar se a administração proativa do fármaco anti-IL-31 lokivetmab poderia prevenir ou espaçar as crises de dermatite DA canina. Nós testamos esta estratégia em quatro cães Maltês-Beagle atópicos antes de incluirmos 21 cães com dermatite atópica espontânea. No nosso modelo de DA aguda, administramos uma injeção de lokivetmab em cães sensibilizados por ácaros da poeira doméstica (APD). Após sete dias, a suspensão de APD foi aplicada por via epicutânea, e ambas as lesões cutâneas e as manifestações de prurido foram quantificadas por 24 horas. No segundo estudo, 21 cães com DA espontânea controlada com drogas antialérgicas foram tratados com lokivetmab de acordo com as recomendações do fabricante; todas as drogas antialérgicas foram descontinuadas dentro de quatro semanas após a primeira injeção. Todos os cães foram acompanhados prospectivamente por até um ano e o tempo até a crise (TAC) de DA a partir do último dia de tratamento antialérgico ter sido administrado. No estudo experimental, uma injeção de lokivetmab foi capaz de prevenir quase todas as manifestações de prurido alérgeno-induzidas esperadas, mas não evitou o desenvolvimento de lesões de pele. Nos cães com DA espontânea, a média de TAC após a terapia proativa com lokivetmab foi de 63 dias. Um quarto dos cães não exibiu sinais clínicos de crise por ao menos um ano enquanto estavam recebendo lokivetmab em monoterapia. Apesar do lokivetmab parecer mais eficaz na prevenção de prurido que lesões cutâneas em cães com DA experimental, ele pode também espaçar as crises da doença em alguns cães com DA espontânea. São necessários mais estudos para identificar aqueles pacientes que podem se beneficiar deste protocolo proativo. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.}, number={2}, journal={Veterinary Dermatology}, publisher={Wiley}, author={Tamamoto‐Mochizuki, Chie and Paps, Judy S. and Olivry, Thierry}, year={2019}, month={Jan}, pages={98–e26} }
 @article{banovic_olivry_baumer_paps_stahl_rogers_jacob_2018, title={Diluted sodium hypochlorite (bleach) in dogs: antiseptic efficacy, local tolerability and in vitro effect on skin barrier function and inflammation}, volume={29}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85040710868&partnerID=MN8TOARS}, DOI={10.1111/vde.12487}, abstractNote={Diluted sodium hypochlorite represents an inexpensive and widely available topical antiseptic, but there are no tolerability and efficacy data in veterinary dermatology.To determine the in vivo antibacterial effect and tolerability of topical diluted bleach application and to assess its in vitro effect on skin barrier lipids and anti-inflammatory properties on keratinocytes.Topical hypochlorite at 0.05% and tap water were applied to both sides of the thorax of four healthy dogs. The anti-inflammatory effect on canine keratinocytes was determined by real-time polymerase chain reaction; skin barrier integrity was assessed by evaluating stratum corneum lipid changes in canine stratified epidermal constructs.The cell viability of primary keratinocytes treated with water and diluted hypochlorite at 0.005 and 0.01%, reduced the percentage of viable cells by 10%. The exposure of primary keratinocytes to 0.005% diluted hypochlorite significantly reduced the induction of inflammatory genes chemokine ligand-2 (CCL2; P = 0.015) and thymus and activation-regulated chemokine (TARC/CCL17, P = 0.032). There were no changes in skin lipid ceramide and nonceramide fractions in stratified epidermal constructs cultured for 17 days with 0.05% hypochlorite. Topical hypochlorite at 0.05% and tap water were well-tolerated without signs of skin irritation. Although a marked reduction in bacterial counts was seen within 20 min of diluted bleach application compared to the tap water control, this was only marginally significant (P = 0.06).The results indicate that a topical diluted bleach solution, at either 0.05 or 0.005% hypochlorite concentrations, is a well-tolerated antiseptic that also exhibits anti-inflammatory properties.}, number={1}, journal={VETERINARY DERMATOLOGY}, author={Banovic, Frane and Olivry, Thierry and Baumer, Wolfgang and Paps, Judy and Stahl, Jessica and Rogers, Ana and Jacob, Megan}, year={2018}, month={Feb} }
 @article{widmer_ferrer_favrot_paps_huynh_olivry_2018, title={Glucocorticosteroids and ciclosporin do not significantly impact canine cutaneous microbiota}, volume={14}, ISSN={["1746-6148"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85042404405&partnerID=MN8TOARS}, DOI={10.1186/s12917-018-1370-y}, abstractNote={As prednisone and ciclosporin can have immunosuppressive effects and have been considered potential predisposing factors for skin infections, we investigated the impact of these drugs on the diversity of the cutaneous microbiota, the abundance of Malassezia and infection with Papillomaviruses. Six atopic, asymptomatic Maltese-beagle dogs were treated with ciclosporin for one month and then with prednisone for another month, with a one-month wash-out between treatments. The dogs were sampled on the abdomen and pinna before and after each treatment using a swab. Samples for Papillomavirus detection were obtained with cytobrush sticks. The bacterial microbiota was characterized using 16S amplicon high-throughput sequencing. Malassezia populations were quantified with nested real-time PCR targeting the ribosomal internal transcribed spacer 1. The diversity and composition of cutaneous microbiota was not impacted in a detectable manner by any of the treatments. As observed for the bacterial microbiota, Malassezia populations were not affected by treatment. Three dogs were positive for Papillomavirus at more than one timepoint, but an association with treatment was not apparent. Ciclosporin and prednisone at doses used for the treatment of atopic dermatitis do not impact the canine cutaneous microbiota in a detectable manner.}, number={1}, journal={BMC VETERINARY RESEARCH}, author={Widmer, Giovanni and Ferrer, Lluis and Favrot, Claude and Paps, Judy and Huynh, Kevin and Olivry, Thierry}, year={2018}, month={Feb} }
 @article{paps_baeumer_olivry_2016, title={Development of an Allergen-induced Atopic Itch Model in Dogs: A Preliminary Report}, volume={96}, ISSN={["1651-2057"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84959099997&partnerID=MN8TOARS}, DOI={10.2340/00015555-2243}, number={3}, journal={ACTA DERMATO-VENEREOLOGICA}, author={Paps, Judy S. and Baeumer, Wolfgang and Olivry, Thierry}, year={2016}, pages={400–401} }
 @article{olivry_mayhew_paps_linder_peredo_rajpal_hofland_cote-sierra_2016, title={Early Activation of Th2/Th22 Inflammatory and Pruritogenic Pathways in Acute Canine Atopic Dermatitis Skin Lesions}, volume={136}, ISSN={["1523-1747"]}, url={https://doi.org/10.1016/j.jid.2016.05.117}, DOI={10.1016/j.jid.2016.05.117}, abstractNote={Determining inflammation and itch pathway activation in patients with atopic dermatitis (AD) is fraught with the inability to precisely assess the age of skin lesions, thus affecting the analysis of time-dependent mediators. To characterize inflammatory events occurring during early experimental acute AD lesions, biopsy samples were collected 6, 24, and 48 hours after epicutaneous application of Dermatophagoides farinae house dust mites to sensitized atopic dogs. The skin transcriptome was assessed using a dog-specific microarray and quantitative PCR. Acute canine AD skin lesions had a significant up-regulation of genes encoding T helper (Th) 2 (e.g., IL4, IL5, IL13, IL31, and IL33), Th9 (IL9), and Th22 (IL22) cytokines as well as Th2-promoting chemokines such as CCL5 and CCL17. Proinflammatory (e.g., IL6, LTB, and IL18) cytokines were also up-regulated. Other known pruritogenic pathways were also activated: there was significant up-regulation of genes encoding proteases cathepsin S (CTSS), mast cell chymase (CMA1), tryptase (TPS1) and mastin, neuromedin-B (NMB), nerve growth factor (NGF), and leukotriene-synthesis enzymes (ALOX5, ALOX5AP, and LTA4H). Experimental acute canine house dust mite-induced AD lesions exhibit an activation of innate and adaptive immune responses and pruritogenic pathways similar to those seen in humans with acute AD, thereby validating this model to test innovative therapeutics modalities for this disease.}, number={10}, journal={JOURNAL OF INVESTIGATIVE DERMATOLOGY}, publisher={Elsevier BV}, author={Olivry, Thierry and Mayhew, David and Paps, Judy S. and Linder, Keith E. and Peredo, Carlos and Rajpal, Deepak and Hofland, Hans and Cote-Sierra, Javier}, year={2016}, month={Oct}, pages={1961–1969} }
 @article{pierezan_olivry_paps_lawhon_wu_steiner_suchodolski_hoffmann_2016, title={The skin microbiome in allergen-induced canine atopic dermatitis}, volume={27}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84980351834&partnerID=MN8TOARS}, DOI={10.1111/vde.12366}, abstractNote={Studies focusing on next-generation sequencing of the bacterial 16S rRNA gene have allowed detailed surveys of skin bacterial populations (microbiota) of the skin.This study evaluated temporal changes in the skin microbiota in a canine model of atopic dermatitis.Eight atopic dogs previously sensitized with house dust mites (HDM).The dogs were topically challenged on the right groin with HDM allergens. Swabs were collected from the challenged and the contralateral nonchallenged sites prior to provocation (pre-challenge; baseline sample) and on days 1, 7, 14, 21 and 28 after allergen challenge. The 16S rRNA gene was amplified, sequenced and analysed. Staphylococcus spp. and Staphylococcus pseudintermedius were quantified with quantitative PCR (RT-qPCR).Skin lesions developed in all dogs on the challenged sites. Differences in bacterial groups were observed on the challenged site over time. Relatively lower abundances of Fusobacteriaceae on Day 7, and, based on LEfSe, increased abundances of Corynebacteriaceae on Day 1, and Staphylococcaceae on days 7, 14 and 21, were observed on the challenged site, compared to the contralateral site. Results of RT-qPCR correlated with those of next-generation sequencing, with significantly increased numbers of Staphylococcus spp. and S. pseudintermedius on Day 21, and days 7 and 21 on the challenged site compared to the contralateral site, respectively.This study demonstrates that an allergen challenge in sensitized dogs leads to bacterial dysbiosis with increased abundance of S. pseudintermedius at the site of lesion induction.}, number={5}, journal={VETERINARY DERMATOLOGY}, author={Pierezan, Felipe and Olivry, Thierry and Paps, Judith S. and Lawhon, Sara D. and Wu, Jing and Steiner, Jorg M. and Suchodolski, Jan S. and Hoffmann, Aline Rodrigues}, year={2016}, month={Oct}, pages={332-+} }
 @article{olivry_bizikova_paps_dunston_lerner_yosipovitch_2013, title={Cowhage can induce itch in the atopic dog}, volume={22}, ISSN={["1600-0625"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84878344758&partnerID=MN8TOARS}, DOI={10.1111/exd.12158}, abstractNote={Itch is a cardinal symptom of atopic dermatitis in humans and dogs. Until now, experimental induction of itch in dogs has proven difficult. The objectives of this study were to determine whether protease-rich spicules, protein extracts and the protease mucunain of the tropical legume cowhage provoked itch and inflammation when rubbed onto canine skin. Native spicules variably induced itch manifestations in about half of the dogs, while challenges with protease-deactivated spicules remained negative. The epicutaneous application of cowhage extract and mucunain after microneedle roller usage also induced pruritus and inflammation. Importantly, there was an interindividual inconsistency in pruritus and inflammation induction and also marked differences in pruritus intensity after challenge. In conclusion, cowhage spicules, protein-rich extracts and mucunain can all induce pruritus and inflammation in dogs as in other species, but the inconsistency of provocation is currently a limitation of this challenge type for future studies of pruritus in dogs.}, number={6}, journal={EXPERIMENTAL DERMATOLOGY}, author={Olivry, Thierry and Bizikova, Petra and Paps, Judy S. and Dunston, Stan and Lerner, Ethan A. and Yosipovitch, Gil}, year={2013}, month={Jun}, pages={435–437} }
 @article{olivry_linder_wang_bizikova_bernstein_dunston_paps_casal_2012, title={Deficient plakophilin-1 expression due to a mutation in PKP1 causes ectodermal dysplasia-skin fragility syndrome in Chesapeake Bay Retriever dogs}, volume={7}, number={2}, journal={PLoS One}, author={Olivry, T. and Linder, K. E. and Wang, P. and Bizikova, P. and Bernstein, J. A. and Dunston, S. M. and Paps, J. S. and Casal, M. L.}, year={2012} }
 @article{stahl_paps_bäumer_olivry_2012, title={Dermatophagoides farinae house dust mite allergen challenges reduce stratum corneum ceramides in an experimental dog model of acute atopic dermatitis}, volume={23}, ISSN={0959-4493}, url={http://dx.doi.org/10.1111/j.1365-3164.2012.01114.x}, DOI={10.1111/j.1365-3164.2012.01114.x}, abstractNote={Ceramides are essential stratum corneum (SC) lipids and they play a pivotal role in maintaining effective cutaneous barrier function.The present study aimed at determining the effect of a Dermatophagoides farinae house dust mite (Df-HDM) allergen challenge on SC ceramides of atopic dogs experimentally sensitized to these allergens.Six Df-HDM-sensitized atopic Maltese-beagle dogs were used.Prechallenge SC was obtained by cyanoacrylate stripping. One week later, the dogs were challenged topically with Df-HDM allergens, which resulted in mild to moderate inflammation 24 h later. Two weeks after challenge, SC of lesional and nonlesional skin was obtained. Finally, SC was collected from challenge sites 2 months after lesion resolution. The different SC lipids were quantified blindly by thin-layer chromatography.Significantly lower amounts of ceramides [AH], [AP], [AS], [NP], [EOP], [NS] and [EOS] were observed in lesional SC compared with prechallenge samples, while no significant effect was found on the amount of other lipids, including cholesterol and free fatty acids. The ceramide profile of nonlesional skin generally showed the same postchallenge reduction pattern. Ceramide amounts returned to normal within 2 months after lesion remission.These findings suggest that the allergic reactions caused by Df-HDM allergens lead to a selective reduction of SC ceramides, not only at sites of inflammation but also at sites away from those of allergen application. There is normalization of ceramide amounts after inflammation subsides. These observations suggest that the deficiency of ceramides observed in canine atopic skin occurs, at least in part, secondary to inflammation.}, number={6}, journal={Veterinary Dermatology}, publisher={Wiley}, author={Stahl, Jessica and Paps, Judy and Bäumer, Wolfgang and Olivry, Thierry}, year={2012}, month={Nov}, pages={497–e97} }
 @article{olivry_linder_paps_bizikova_dunston_donne_mondoulet_2012, title={Validation of a novel epicutaneous delivery system for patch testing of house dust mite-hypersensitive dogs}, volume={23}, number={6}, journal={Veterinary Dermatology}, author={Olivry, T. and Linder, K. E. and Paps, J. S. and Bizikova, P. and Dunston, S. and Donne, N. and Mondoulet, L.}, year={2012} }
 @misc{olivry_paps_2011, title={Evaluation of the agreement between allergen-specific intradermal or IgE serological tests and a point-of-care immunodot assay in dogs with atopic dermatitis}, volume={22}, ISSN={["0959-4493"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-79955646421&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2010.00936.x}, abstractNote={Veterinary DermatologyVolume 22, Issue 3 p. 284-285 Letter to the Editor Evaluation of the agreement between allergen-specific intradermal or IgE serological tests and a point-of-care immunodot assay in dogs with atopic dermatitis Thierry Olivry, Thierry Olivry Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University,Raleigh, NC, USA Center for Comparative Medicine and Translational Research, North Carolina State University,Raleigh, NC, USASearch for more papers by this authorJudy Paps, Judy Paps Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University,Raleigh, NC, USASearch for more papers by this author Thierry Olivry, Thierry Olivry Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University,Raleigh, NC, USA Center for Comparative Medicine and Translational Research, North Carolina State University,Raleigh, NC, USASearch for more papers by this authorJudy Paps, Judy Paps Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University,Raleigh, NC, USASearch for more papers by this author First published: 11 November 2010 https://doi.org/10.1111/j.1365-3164.2010.00936.xCitations: 6 Thierry Olivry, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA. E-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume22, Issue3June 2011Pages 284-285 RelatedInformation}, number={3}, journal={VETERINARY DERMATOLOGY}, author={Olivry, Thierry and Paps, Judy}, year={2011}, month={Jun}, pages={284–285} }
 @article{baeumer_stahl_sander_petersen_paps_stark_kietzmann_olivry_2011, title={Lack of preventing effect of systemically and topically administered histamine H-1 or H-4 receptor antagonists in a dog model of acute atopic dermatitis}, volume={20}, ISSN={["0906-6705"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-79959528878&partnerID=MN8TOARS}, DOI={10.1111/j.1600-0625.2011.01268.x}, abstractNote={As there is evidence for an anti-inflammatory efficacy of histamine H4 receptor (H4R) selective antagonists, we aimed at testing the efficacy of the H4R antagonists JNJ7777120 and JNJ28307474 in comparison with histamine H1 receptor (H1R) antagonists hydroxyzine and cetirizine for skin lesion prevention in a canine model of acute atopic dermatitis. Six atopic Maltese-beagle dogs experimentally sensitized to Dermatophagoides farinae (Df) house dust mites were selected for this study. Twenty-four hours after challenge by epicutaneous application of Df, erythematous skin lesions were scored. In this blinded, placebo and active controlled study, topical JNJ7777120 or JNJ28307474 was applied as a 1% solution before allergen challenge. The latter was also given orally at 15 mg/kg before and after allergen challenge. A 0.015% triamcinolone acetonide solution was used as a positive control. The H1R antagonists hydroxyzine and cetirizine were administered orally before challenge in a second experiment. Twenty-four hours after challenge, placebo-treated animals had a median lesional score of 2. Treatment with topical and oral JNJ28307474 resulted in a median score of 2.5. After topical administration of JNJ7777120, the median lesional score was 2. Hydroxyzine and cetirizine did also not reduce the median score of the placebo treatment. Triamcinolone acetonide prevented all dogs from having any lesions. Determination of histamine in lesions revealed that only during the initiation increased concentrations of histamine were detected. In conclusion, the preventive administration of H1R or H4R antagonists has no impact on the development of acute skin lesions in this experimental canine atopic dermatitis model. Figure S1. Amount of permeated JNJ7777120 (A) and JNJ28307474 (B) in the acceptor medium after topical administration of a 1% formulation on dog skin (mean ± SD of three different skin samples/each experiment). Figure S2. Concentration of histamine in the microdialysate after epicutaneous administration of Df antigen. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.}, number={7}, journal={EXPERIMENTAL DERMATOLOGY}, author={Baeumer, Wolfgang and Stahl, Jessica and Sander, Kerstin and Petersen, Lars J. and Paps, Judy and Stark, Holger and Kietzmann, Manfred and Olivry, Thierry}, year={2011}, month={Jul}, pages={577–581} }
 @article{olivry_wofford_paps_dunston_2011, title={Stratum corneum removal facilitates experimental sensitization to mite allergens in atopic dogs}, volume={22}, ISSN={["1365-3164"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-79952348135&partnerID=MN8TOARS}, DOI={10.1111/j.1365-3164.2010.00938.x}, abstractNote={In humans with atopic dermatitis and in mouse models of IgE-mediated allergic diseases, evidence is mounting that the stratum corneum (SC) provides an important barrier against environmental allergens. At this time, it is not known whether the SC has a similar role in dogs, especially in those with atopic dermatitis. The objectives of this pilot study were to determine whether SC removal led to earlier and stronger sensitization of atopic dogs to Dermatophagoides farinae (Df) house dust mites. Five Maltese-beagle atopic (MBA) dogs were sensitized epicutaneously after the SC was removed with ten tape strips (TS group), while sensitization was done without tape strips in five other MBA dogs (nontape stripping; NTS group). During this 16 week study, sensitization was assessed with allergen-specific IgE serology, intradermal testing with Df allergens and determination of stimulation indices of blood mononuclear cells cultured with Df and stained for CD4 and the activation markers CD25 or CD30. Compared with dogs from the NTS group, those of the TS group exhibited earlier rises in Df-specific IgE serum levels, usually had higher allergen-specific IgE titres, showed higher intradermal test reactivity and had earlier increases and higher percentages of CD25- or CD30-positive activated allergen-specific peripheral CD4-positive T lymphocytes. These observations implicate a role of the SC as a barrier limiting sensitization to exogenous allergens in this experimental atopic dog model. Dans la dermatite atopique humaine et les modèles murins de maladies allergiques médiées par les IgE, il est prouvé que le stratum corneum (SC) joue un rôle important de barrière contre les allergènes environnementaux. A ce jour, nous ne savons pas si le SC a un rôle identique chez le chien, en particulier chez les chiens atopiques. Les objectifs de cette étude pilote sont de déterminer si le retrait du SC entraîne une sensibilisation plus précoce et plus intense des chiens atopiques aux acariens de la poussière de maison Dermatophagoides farinae (Df). Cinq beagles maltais atopiques (MBA) ont été sensibilisés par voie épicutanée après que le SC ait été retiréà l’aide de 10 rubans adhésifs (groupe TS). La sensibilisation était réalisée parallèlement chez cinq autres chiens MBA, sans ruban adhésif (groupe NTS-Non tape stripping). Au cours des 16 semaines d’étude, la sensibilisation était évaluée par des sérologies d’IgE spécifiques d’allergènes, des tests intradermiques avec Df et la mise en évidence des indices de stimulation des cellules mononuclées sanguines cultivées avec Df et marquées pour CD4 et les marqueurs d’activation CD25 et CD30. En comparaison avec les chiens du groupe NTS, les chiens du groupe TS ont montré une élévation des taux sériques d’IgE spécifiques de Df plus précoces et en général plus élevés, montraient une réactivité plus importante aux tests intradermiques et avaient une augmentation plus rapide et plus haute des pourcentages de lymphocytes CD4+ périphériques spécifiques d’allergènes activés CD25 ou CD30+. Ces observations sont en faveur du rôle de barrière du SC, limitant la sensibilisation aux allergènes exogènes dans ce modèle expérimental de chiens atopiques. En seres humanos con dermatitis atópica y en modelos murinos de enfermedades alérgicas mediadas por IgE, existen evidencias acumuladas que el estrato corneo (SC) confiere una barrera importante frente a alergenos ambientales. En estos momentos no se sabe si el SC tiene una función similar en perros, especialmente en perros con dermatitis atópica. Los objetivos de este estudio piloto fueron determinar si la retirada del SC producía una sensitización más rápida y más intensa en perros atópicos a ácaros del polvo Dermatophagoides farinae (Df). Cinco perros atópicos cruzados Maltes-Beagle (MBA) fueron sensitizados por vía epicutánea tras remover el SC con diez pegadas de cinta adhesiva (grupo TS) mientras que la sensitización se realizó sin TS en otros cinco perros MBA (perros sin cinta adhesiva, grupo NTS). Durante las 16 semanas de estudio la sensitización se evaluó mediante serología para IgE específicas, prueba intradermal con alergenos de Df y tinción para linfocitos CD4 y marcadores de activación CD25 o CD30. Comparados con los perros del grupo NTS, los perros del grupo TS presentaron elevaciones mas tempranas en los niveles de IgE específicos para Df, tenían normalmente títulos mas elevados de IgE, mostraron mayor reactividad en las pruebas intradérmicas, y tuvieron incrementos mas tempranos y mayores porcentajes de linfocitos CD4 periféricos específicos de alergeno positivos para CD25 o CD30. Estas observaciones indican un papel del SC como barrera limitante para la sensitización a alergenos exógenos en este modelo experimental de atopia canina. Bei Menschen mit atopischer Dermatitis und bei Mausmodellen mit IgE-mediierten allergischen Erkrankungen besteht zunehmende Evidenz dafür, dass das Stratum corneum (SC) eine wichtige Barriere gegenüber Umweltallergenen darstellt. Zum jetzigen Zeitpunkt ist es nicht bekannt, ob SC bei Hunden, vor allem bei jenen mit atopischer Dermatitis, eine ähnliche Rolle spielen. Die Ziele dieser Pilotstudie waren es, zu bestimmen, ob die Entfernung des SC zu einer früheren und stärkeren Sensibilisierung der atopischen Hunde auf Dermatophagoides farinae (Df) Hausstaubmilben führen würde. Fünf atopische Malteser-Beagles (MBA) wurden, nachdem das SC mit zehn Klebestreifen (TS Gruppe) entfernt worden war, epikutan sensibilisiert, während eine Sensibilisierung bei fünf anderen MBA Hunden ohne TS (Keine Klebestreifenentfernung - NTS - Gruppe) durchgeführt wurde. Während dieser 16 wöchigen Studie wurde die Sensibilisierung mittels IgE Serologie, mittels Intradermaltest mit Df Allergenen und durch die Bestimmung von Stimulationsindizes der mononukleären Zellen im Blut, die mit Df kultiviert und gegen CD4, sowie gegen die Aktivierungsmarker CD25 oder CD30 gefärbt worden waren, beurteilt. Im Vergleich zu den Hunden in der NTS Gruppe zeigten jene der TS Gruppe frühere Anstiege der Df-spezifischen IgE Serumwerte, sie hatten üblicherweise höhere Allergen-spezifische IgE Titer, sie zeigten eine höhere Reaktivität im Intradermaltest und hatten frühere Zunahmen und höhere Prozentanteile an CD25 oder CD30-positiven aktivierten Allergen-spezifischen peripheren CD4-positiven T-Lymphozyten. Diese Beobachtungen ermöglichen eine Argumentation für das SC als eine Barriere, die die Sensibilisierung gegenüber exogenen Allergenen in diesem experimentellen Modell atopischer Hunde limitiert.}, number={2}, journal={VETERINARY DERMATOLOGY}, author={Olivry, Thierry and Wofford, Jessica and Paps, Judy S. and Dunston, Stanley M.}, year={2011}, month={Apr}, pages={188–196} }
 @article{ricci_hammerberg_paps_contiero_jackson_2010, title={A comparison of the clinical manifestations of feeding whole and hydrolysed chicken to dogs with hypersensitivity to the native protein}, volume={21}, ISSN={["1365-3164"]}, DOI={10.1111/j.1365-3164.2010.00871.x}, abstractNote={Twenty-six dogs with known adverse food reactions were fed whole chicken for 14 days. From this group, 12 dogs with cutaneous manifestations following exposure to chicken meat were selected and randomly divided into two groups (n = 6). Each group was then fed hydrolysed chicken or hydrolysed soy for 14 days in a blinded crossover design with a 17-day washout period between each diet. Assessments of a CADESI (Canine Atopic Dermatitis Extent and Severity Index) score and pruritus were performed throughout the entire study, and combined in a global score (GS). Serum was collected weekly for the measurement of chicken- and soy-specific IgG and IgE. Dogs displayed the most severe clinical response when eating whole chicken compared to baseline (P < 0.001). The GS was significantly reduced in 11 of the 12 dogs when fed hydrolysed chicken were compared to those fed whole chicken (3.58 ± 2.81 versus 20.38 ± 14.65, P < 0.01). Serum immunoglobulin G and E responses were variable and did not show relationship with specific dietary exposure.}, number={4}, journal={VETERINARY DERMATOLOGY}, author={Ricci, Rebecca and Hammerberg, Bruce and Paps, Judy and Contiero, Barbara and Jackson, Hilary}, year={2010}, month={Aug}, pages={358–366} }
 @article{bizikova_linder_paps_olivry_2010, title={Effect of a novel topical diester glucocorticoid spray on immediate- and late-phase cutaneous allergic reactions in Maltese-beagle atopic dogs: a placebo-controlled study}, volume={21}, number={1}, journal={Veterinary Dermatology}, author={Bizikova, P. and Linder, K. E. and Paps, J. and Olivry, T.}, year={2010}, pages={70–79} }
 @article{olivry_kurata_paps_masuda_2007, title={A blinded randomized controlled trial evaluating the usefulness of a novel diet (Aminoprotect Care) in dogs with spontaneous food allergy}, volume={69}, ISSN={["1347-7439"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-36248967710&partnerID=MN8TOARS}, DOI={10.1292/jvms.69.1025}, abstractNote={Aminoprotect Care (APC) is a novel diet composed of aminoacids, potato proteins and corn starch. The objectives of this study were to determine whether Maltese-Beagle atopic (MBA) dogs hypersensitive to corn exhibited clinical signs and changes in immunological markers after being fed APC. The study was designed as a blinded randomized controlled crossover experiment. Ten MBA dogs with signs of allergy within five days of ingesting corn were selected. Dogs were randomized to be fed either their maintenance diet with corn or APC for five days. After a washout of two weeks, diets were switched. Before and daily during each intervention, skin lesions were graded by an investigator while pruritus was assessed by another. Before and at the end of each intervention, the percentage of circulating CD4+CCR4+, corn-activated CD4+ T-lymphocytes and serum corn-specific IgE levels were measured and ratios of post:pre values calculated. During this trial, pruritus and skin lesions increased significantly in MBA dogs when ingesting corn while no such increase occurred when fed APC. Total, median and maximal pruritus values were significantly higher in MBA dogs ingesting corn compared to APC. There were no significant differences between interventions in the immunological parameters assessed. In summary, even though APC contains corn starch to which corn-sensitive MBA dogs often react, the ingestion of APC did not lead to significant increases in skin lesions or pruritus. Aminoprotect Care might prove valuable for management of food allergies. These experimental observations must be validated in large field studies.}, number={10}, journal={JOURNAL OF VETERINARY MEDICAL SCIENCE}, author={Olivry, Thierry and Kurata, Keigo and Paps, Judy S. and Masuda, Kenichi}, year={2007}, month={Oct}, pages={1025–1031} }
 @misc{olivry_paps_bizikova_murphy_jackson_zebala_2007, title={A pilot open trial evaluating the efficacy of low-dose aminopterin in the canine homologue of human atopic dermatitis}, volume={157}, ISSN={["1365-2133"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-35348885050&partnerID=MN8TOARS}, DOI={10.1111/j.1365-2133.2007.08133.x}, abstractNote={Journal Article A pilot open trial evaluating the efficacy of low‐dose aminopterin in the canine homologue of human atopic dermatitis Get access T. Olivry, T. Olivry Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, U.S.A.Allergy Core, Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar J.S. Paps, J.S. Paps Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar P. Bizikova, P. Bizikova Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar K.M. Murphy, K.M. Murphy Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar H.A. Jackson, H.A. Jackson Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, U.S.A. Search for other works by this author on: Oxford Academic Google Scholar J. Zebala J. Zebala Syntrix Biosystems, Auburn, WA, U.S.A. E‐mail: Thierry_Olivry@ncsu.edu Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology, Volume 157, Issue 5, 1 November 2007, Pages 1040–1042, https://doi.org/10.1111/j.1365-2133.2007.08133.x Published: 01 November 2007}, number={5}, journal={BRITISH JOURNAL OF DERMATOLOGY}, author={Olivry, T. and Paps, J. S. and Bizikova, P. and Murphy, K. M. and Jackson, H. A. and Zebala, J.}, year={2007}, month={Nov}, pages={1040–1042} }
 @article{tater_jackson_paps_hammerberg_2005, title={Effects of routine prophylactic vaccination or administration of aluminum adjuvant alone on allergen-specific serum IgE and IgG responses in allergic dogs}, volume={66}, ISSN={["0002-9645"]}, DOI={10.2460/ajvr.2005.66.1572}, abstractNote={Abstract Objective —To determine the acute corn-specific serum IgE and IgG, total serum IgE, and clinical responses to SC administration of prophylactic vaccines and aluminum adjuvant in corn-allergic dogs. Animals —20 allergic and 8 nonallergic dogs. Procedure —17 corn-allergic dogs were vaccinated. Eight clinically normal dogs also were vaccinated as a control group. Serum corn-specific IgE, corn-specific IgG, and total IgE concentrations were measured in each dog before vaccination and 1 and 3 weeks after vaccination by use of an ELISA. The corn-allergic dogs also had serum immunoglobulin concentrations measured at 8 and 9 weeks after vaccination. Twenty allergic dogs received a SC injection of aluminum adjuvant, and serum immunoglobulin concentrations were measured in each dog 1, 2, 3, 4, and 8 weeks after injection. The allergic dogs were examined during the 8 weeks after aluminum administration for clinical signs of allergic disease. Results —The allergic dogs had significant increases in serum corn-specific IgE and IgG concentrations 1 and 3 weeks after vaccination but not 8 or 9 weeks after vaccination. Control dogs did not have a significant change in serum immunoglobulin concentrations after vaccination. After injection of aluminum adjuvant, the allergic dogs did not have a significant change in serum immunoglobulin concentrations or clinical signs. Conclusions and Clinical Relevance —Allergen-specific IgE and IgG concentrations increase after prophylactic vaccination in allergic dogs but not in clinically normal dogs. Prophylactic vaccination of dogs with food allergies may affect results of serologic allergen-specific immunoglobulin testing performed within 8 weeks after vaccination. ( Am J Vet Res 2005;66:1572–1577)}, number={9}, journal={AMERICAN JOURNAL OF VETERINARY RESEARCH}, author={Tater, KC and Jackson, HA and Paps, J and Hammerberg, B}, year={2005}, month={Sep}, pages={1572–1577} }