@article{sampson_muddiman_2009, title={Atmospheric pressure infrared (10.6 mu m) laser desorption electrospray ionization (IR-LDESI) coupled to a LTQ Fourier transform ion cyclotron resonance mass spectrometer}, volume={23}, ISSN={["1097-0231"]}, DOI={10.1002/rcm.4113}, abstractNote={We present the implementation of a CO(2) laser emitting infrared laser irradiation at 10.6 microm onto the versatile atmospheric pressure ionization platform. Infrared laser desorption electrospray ionization (IR-LDESI) is demonstrated from liquid-state samples at atmospheric pressure with and without ESI postionization. Multiply charged proteins ranging in molecular mass from 8.6 to 17 kDa were detected from liquid-state samples without the addition of matrix.}, number={13}, journal={RAPID COMMUNICATIONS IN MASS SPECTROMETRY}, author={Sampson, Jason S. and Muddiman, David C.}, year={2009}, month={Jul}, pages={1989–1992} }
 @article{dixon_sampson_muddiman_2009, title={Generation of Multiply Charged Peptides and Proteins by Radio Frequency Acoustic Desorption and Ionization for Mass Spectrometric Detection}, volume={20}, ISSN={["1879-1123"]}, DOI={10.1016/j.jasms.2008.11.024}, abstractNote={The design and implementation of a radio frequency acoustic desorption ionization (RADIO) source has been demonstrated for the analysis of multiply charged peptides and proteins. One muL aliquots of melittin, BNP-32, and ubiquitin ( approximately 1 mug of analyte) were deposited onto a quartz crystal microbalance (QCM) electrode before radio frequency actuation for desorption. Continuous electrospray parallel to/above the sampling surface enabled the ionization of desorbed species. Detection by a hybrid linear ion trap Fourier transform ion cyclotron resonance mass spectrometer confirmed the intact and dissociated species observed during MS and MS/MS experiments, respectively.}, number={4}, journal={JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY}, author={Dixon, R. Brent and Sampson, Jason S. and Muddiman, David C.}, year={2009}, month={Apr}, pages={597–600} }
 @article{sampson_murray_muddiman_2009, title={Intact and Top-Down Characterization of Biomolecules and Direct Analysis Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Coupled to FT-ICR, Mass Spectrometry}, volume={20}, ISSN={["1879-1123"]}, DOI={10.1016/j.jasms.2008.12.003}, abstractNote={We report the implementation of an infrared laser onto our previously reported matrix-assisted laser desorption electrospray ionization (MALDESI) source with ESI post-ionization yielding multiply charged peptides and proteins. Infrared (IR)-MALDESI is demonstrated for atmospheric pressure desorption and ionization of biological molecules ranging in molecular weight from 1.2 to 17 kDa. High resolving power, high mass accuracy single-acquisition Fourier transform ion cyclotron resonance (FT-ICR) mass spectra were generated from liquid- and solid-state peptide and protein samples by desorption with an infrared laser (2.94 mum) followed by ESI post-ionization. Intact and top-down analysis of equine myoglobin (17 kDa) desorbed from the solid state with ESI post-ionization demonstrates the sequencing capabilities using IR-MALDESI coupled to FT-ICR mass spectrometry. Carbohydrates and lipids were detected through direct analysis of milk and egg yolk using both UV- and IR-MALDESI with minimal sample preparation. Three of the four classes of biological macromolecules (proteins, carbohydrates, and lipids) have been ionized and detected using MALDESI with minimal sample preparation. Sequencing of O-linked glycans, cleaved from mucin using reductive beta-elimination chemistry, is also demonstrated.}, number={4}, journal={JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY}, author={Sampson, Jason S. and Murray, Kermit K. and Muddiman, David C.}, year={2009}, month={Apr}, pages={667–673} }
 @article{fields-zinna_sampson_crowe_tracy_parker_deney_muddiman_murray_2009, title={Tandem Mass Spectrometry of Thiolate-Protected Au Nanoparticles NaxAu25(SC2H4Ph)(18-y)(S(C2H4O)(5)CH3)(y)}, volume={131}, ISSN={["0002-7863"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000270186600057&KeyUID=WOS:000270186600057}, DOI={10.1021/ja905787y}, abstractNote={We report the first collision-induced dissociation tandem mass spectrometry (CID MS/MS) of a thiolate-protected Au nanoparticle that has a crystallographically determined structure. CID spectra assert that dissociation pathways for the mixed monolayer Na(x)Au(25)(SC(2)H(4)Ph)(18-y)(S(C(2)H(4)O)(5)CH(3))(y) centrally involve the semi-ring Au(2)L(3) coordination (L = some combination of the two thiolate ligands) that constitutes the nanoparticle's protecting structure. The data additionally confirm charge state assignments in the mass spectra. Prominent among the fragments is [Na(2)AuL(2)](1+), one precursor of which is identified as another nanoparticle fragment in the higher m/z region. Another detected fragment, [Na(2)Au(2)L(3)](1+), represents a mass loss equivalent to an entire semi-ring, whereas others suggest involvement (fragmentation/rearrangement) of multiple semi-rings, e.g., [NaAu(3)L(3)](1+) and [NaAu(4)L(4)](1+). The detailed dissociation/rearrangement mechanisms of these species are not established, but they are observed in other mass spectrometry experiments, including those under non-CID conditions, namely, electrospray ionization mass spectrometry (ESI-MS) with both time-of-flight (TOF) and FT-ICR analyzers. The latter, previously unreported results show that even soft ionization sources can result in Au nanoparticle fragmentation, including that yielding Au(4)L(4) in ESI-TOF of a much larger thiolate-protected Au(144) nanoparticle under non-CID conditions.}, number={38}, journal={JOURNAL OF THE AMERICAN CHEMICAL SOCIETY}, author={Fields-Zinna, Christina A. and Sampson, Jason S. and Crowe, Matthew C. and Tracy, Joseph B. and Parker, Joseph F. and deNey, Alexander M. and Muddiman, David C. and Murray, Royce W.}, year={2009}, month={Sep}, pages={13844–13851} }
 @article{dixon_sampson_hawkridge_muddiman_2008, title={Ambient aerodynamic ionization source for remote analyte sampling and mass spectrometric analysis}, volume={80}, ISSN={["1520-6882"]}, DOI={10.1021/ac800289f}, abstractNote={The use of aerodynamic devices in ambient ionization source development has become increasingly prevalent in the field of mass spectrometry. In this study, an air ejector has been constructed from inexpensive, commercially available components to incorporate an electrospray ionization emitter within the exhaust jet of the device. This novel aerodynamic device, herein termed remote analyte sampling, transport, and ionization relay (RASTIR) was used to remotely sample neutral species in the ambient and entrain them into an electrospray plume where they were subsequently ionized and detected using a linear ion trap Fourier transform mass spectrometer. Two sets of experiments were performed in the ambient environment to demonstrate the device's utility. The first involved the remote (approximately 1 ft) vacuum collection of pure sample particulates (i.e., dry powder) from a glass slide, entrainment and ionization at the ESI emitter, and mass spectrometric detection. The second experiment involved the capture (vacuum collection) of matrix-assisted laser desorbed proteins followed by entrainment in the ESI emitter plume, multiple charging, and mass spectrometric detection. This approach is in principle a RASTIR-assisted matrix-assisted laser desorption electrospray ionization source (Sampson, J. S.; Hawkridge, A. M.; Muddiman, D. C. J. Am. Soc. Mass Spectrom. 2006, 17, 1712-1716; Rapid Commun. Mass Spectrom. 2007, 21, 1150-1154.). A detailed description of the device construction, operational parameters, and preliminary small molecule and protein data are presented.}, number={13}, journal={ANALYTICAL CHEMISTRY}, author={Dixon, R. Brent and Sampson, Jason S. and Hawkridge, Adam M. and Muddiman, David C.}, year={2008}, month={Jul}, pages={5266–5271} }
 @article{sampson_hawkridge_muddiman_2008, title={Construction of a versatile high precision ambient Ionization source for direct analysis and imaging}, volume={19}, DOI={10.1016/j.jasms.2008.06.013}, abstractNote={The design and construction of a high precision ambient ionization source matrix-assisted laser desorption electrospray ionization (MALDESI) are described in full detail, including a complete parts list. The computer controlled high precision motion control system and high repetition rate Explorer laser are demonstrated during MALDESI-FT-ICR analysis of peptides and proteins ranging from 1 to 17 kDa. The high stability ionization source platform described herein demonstrates both the advantages of the new MALDESI source and versatility for application to numerous desorption and ionization techniques.}, number={10}, journal={Journal of the American Society for Mass Spectrometry}, author={Sampson, J. S. and Hawkridge, A. M. and Muddiman, David}, year={2008}, pages={1527–1534} }
 @article{sampson_hawkridge_muddiman_2008, title={Development and characterization of an ionization technique for analysis of biological macromolecules: Liquid matrix-assisted laser desorption electrospray ionization}, volume={80}, ISSN={["0003-2700"]}, DOI={10.1021/ac8001935}, abstractNote={We have developed an atmospheric pressure ionization technique called liquid matrix-assisted laser desorption electrospray ionization (liq-MALDESI) for the generation of multiply charged ions by laser desorption from liquid samples deposited onto a stainless steel sample target biased at a high potential. This variant of our previously reported MALDESI source does not utilize an ESI emitter to postionize neutrals. Conversely, we report desorption and ionization from a macroscopic charged droplet. We demonstrate high mass resolving power single-acquisition FT-ICR-MS analysis of peptides and proteins ranging from 1 to 8.6 kDa at atmospheric pressure. The liquid sample acts as a macroscopic charged droplet similar to those generated by electrospray ionization, whereby laser irradiation desorbs analyte from organic matrix containing charged droplets generating multiply charged ions. We have observed a singly charged radical cation of an electrochemically active species indicating oxidation occurs for analytes and therefore water; the latter would play a key role in the mechanism of ionization. Moreover, we demonstrate an increase in ion abundance and a concurrent decrease in surface tension with an increase in the applied potential.}, number={17}, journal={ANALYTICAL CHEMISTRY}, author={Sampson, Jason S. and Hawkridge, Adam M. and Muddiman, David C.}, year={2008}, month={Sep}, pages={6773–6778} }
 @article{sampson_hawkridge_muddiman_2007, title={Direct characterization of intact polypeptides by matrix-assisted laser desorption electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometry}, volume={21}, ISSN={["1097-0231"]}, DOI={10.1002/rcm.2947}, abstractNote={We report the characterization of a recently introduced hybrid ionization source, matrix-assisted laser desorption electrospray ionization (MALDESI), coupled to a quadrupole Fourier transform ion cyclotron resonance mass spectrometry (QFT-ICR-MS) system. We first demonstrate the ability of MALDESI-QFT-ICR MS to directly analyze and provide high mass measurement accuracy (approximately 1 part-per-million) of a polypeptide using internal calibration. Second, we show the potential of MALDESI-QFT-ICR MS for the top-down characterization of multiply charged polypeptide cations. Finally, we demonstrate sub-femtomole detection limits in MALDESI-QFT-ICR MS using a combination of naturally occurring peptides and their respective stable isotope labeled forms. The results presented herein demonstrate the feasibility of several potential applications for MALDESI-QFT-ICR MS for the direct analysis of intact biological molecules.}, number={7}, journal={RAPID COMMUNICATIONS IN MASS SPECTROMETRY}, author={Sampson, Jason S. and Hawkridge, Adam M. and Muddiman, David C.}, year={2007}, pages={1150–1154} }
 @article{sampson_hawkridge_muddiman_2006, title={Generation and detection of multiply-charged peptides and proteins by matrix-assisted laser desorption electrospray ionization (MALDESI) Fourier transform ion cyclotron resonance mass spectrometry}, volume={17}, ISSN={["1879-1123"]}, DOI={10.1016/j.jasms.2006.08.003}, abstractNote={We report the coupling of a hybrid ionization source, matrix-assisted laser desorption electrospray ionization (MALDESI), to a Fourier transform-ion cyclotron resonance mass spectrometer (FT-ICR MS).The details of the source design and initial data are presented.Analysis of peptides and proteins ranging from 1 to 8.6 kDa resulted in high resolving power single-acquisition FT-ICR mass spectra with average charge-states highly correlated to those obtained by nanoESI, thus, providing strong evidence that the ESI process dictates the observed charge-state distribution.Importantly, unlike the recently introduced electrospray assisted laser desorption ionization (ELDI) source reported by Shiea and coworkers [1,2], the data we have obtained to date rely on the use of an organic acid matrix.The results presented herein provide insight into the charging mechanism of this emerging ionization approach, while also expanding the utility of FT-ICR MS for top-down protein and complex mixture analysis.(}, number={12}, journal={JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY}, author={Sampson, Jason S. and Hawkridge, Adam M. and Muddiman, David C.}, year={2006}, month={Dec}, pages={1712–1716} }