@article{baker_duarte_kim_2024, title={Effects of dietary xylanase supplementation on growth performance, intestinal health, and immune response of nursery pigs fed diets with reduced metabolizable energy}, volume={102}, ISSN={["1525-3163"]}, url={https://doi.org/10.1093/jas/skae026}, DOI={10.1093/jas/skae026}, abstractNote={Abstract
               This study aimed to investigate the effects of xylanase on growth performance and intestinal health of nursery pigs fed diets with reduced metabolizable energy (ME). One hundred ninety-two pigs at 8.7 kg ± 0.7 body weight (BW) after 7 d of weaning were allotted in a randomized complete block design with initial BW and sex as blocks. Eight dietary treatments consisted of 5 ME levels (3,400, 3,375, 3,350, 3,325, and 3,300 kcal ME/kg) below the NRC (2012) requirement and 4 levels of xylanase (0, 1,200, 2,400, and 3,600 XU/kg) to a diet with 3,300 kcal ME/kg. All pigs received their respective treatments for 35 d in 2 phases, pre-starter (14 d) and starter (21 d). On day 35, eight pigs in 3,400 kcal/kg (CON), 3,300 kcal/kg (LE), and 3,300 kcal/kg + 3,600 XU xylanase/kg (LEX) were euthanized to collect jejunal tissues and digesta for the evaluation of mucosa-associated microbiota, intestinal immune response, oxidative stress status, intestinal morphology, crypt cell proliferation, and digesta viscosity as well as ileal digesta to measure apparent ileal digestibility. Data were analyzed using the MIXED procedure on SAS 9.4. The LE increased (P < 0.05) jejunal digesta viscosity, tended to have decreased (P = 0.053) relative abundance of Prevotella, and tended to increase (P = 0.055) Lactobacillus. The LE also increased (P < 0.05) the concentration of protein carbonyl whereas malondialdehyde, villus height (VH), villus height to crypt depth ratio (VH:CD), apparent ileal digestibility (AID) of nutrients, and finally average daily feed intake were decreased (P < 0.05). The LE did not affect average daily gain (ADG). The LEX decreased (P < 0.05) digesta viscosity, increased (P < 0.05) the relative abundance of Prevotella, decreased (P < 0.05) Helicobacter, decreased (P < 0.05) the concentration of protein carbonyl, tended to increase (P = 0.065) VH, and decreased (P < 0.05) VH:CD and crypt cell proliferation. Moreover, LEX increased (P < 0.05) the AID of dry matter and gross energy and tended to increase (P = 0.099; P = 0.076) AID of crude protein, and ether extract. The LEX did not affect ADG but did tend to decrease (P = 0.070) fecal score during the starter phase. Overall, reducing ME negatively affected intestinal health parameters and nutrient digestibility without affecting growth. Supplementation of xylanase mitigated some of the negative effects observed by ME reduction on intestinal health and digestibility of nutrients without affecting growth.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Baker, Jonathan T. and Duarte, Marcos Elias and Kim, Sung Woo}, year={2024}, month={Jan} }
 @article{baker_sokale_frederick_kim_2024, title={Investigation of the nutritional and functional roles of mannanase on intestinal health and growth of newly weaned pigs fed a low-cost formulated diet}, volume={102}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skae102.092}, abstractNote={Abstract This study aimed to investigate the nutritional and functional roles of mannanase on the intestinal health and growth of newly weaned pigs fed a typical or low-cost formulated diet reducing the inclusion of highly digestible protein ingredients. Twenty-four newly weaned pigs at 6.2 ± 0.5 kg initial body weight (BW) were allotted to three dietary treatments based on a randomized complete block design with sex and BW as blocks. Pigs were fed for 37 d in three phases (P1, P2, and P3) and weighed at the end of each phase. Low-cost formulation was achieved by reducing the use of animal protein (fish meal, poultry meal, and blood plasma) and processed soybean meal whereas increasing the use of soybean meal from 13% in typical diets to 28% in P1, from 15% to 32% in P2, and 20.5% to 35% in P3. Treatments were 1) control diet (CON, typical nursery feed), 2) low-cost formulated diet (LCD), and 3) LCD + mannanase inclusion (0 vs. 100 g/t of Natupulse, BASF, providing 800 Thermostable Mannanase Unit, TMU, per ton of feed). On d 37 of feeding, all pigs were euthanized and jejunal mucosal tissues were taken to analyze intestinal health parameters, and gene expression of tight junction proteins and mucins. Pigs fed LCD had reduced (P < 0.05) average daily gain (ADG) and average daily feed intake (ADFI) during the overall period and increased (P < 0.05) fecal score during P3. The LCD decreased (P < 0.05) malondialdehyde and the gene expression of Zona occludens-1 (ZO-) in the jejunum and increased (P < 0.05) serum insulin. The inclusion of mannanase in LCD tended to increase (P = 0.059) BW at d 37, increased (P < 0.05) ADFI during P1, increased (P < 0.05) ADG and gain to feed ratio (G:F) during the overall period, reduced (P < 0.05) fecal score during the overall period, and decreased (P < 0.05) the viscosity of jejunal digesta. However, no effects were observed on intestinal immune response at d 37. In conclusion, feeding LCD negatively affected growth performance and intestinal health of nursery pigs. The inclusion of mannanase to the LCD (800 TMU/t feed) mitigated the negative effects of lowering feed quality observed in growth performance by reducing digesta viscosity and fecal score.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Baker, Jonathan T. and Sokale, Adebayo O. and Frederick, Brent and Kim, Sung Woo}, year={2024}, month={May}, pages={82–82} }
 @article{baker_deng_sokale_frederick_kim_2024, title={Nutritional and functional roles of β-mannanase on intestinal health and growth of newly weaned pigs fed two different types of feeds}, volume={102}, ISSN={["1525-3163"]}, url={https://doi.org/10.1093/jas/skae206}, DOI={10.1093/jas/skae206}, abstractNote={Abstract This study aimed to investigate the nutritional and functional roles of β-mannanase on the intestinal health and growth of newly weaned pigs fed a typical or low-cost formulated feeds (LCF). Twenty-four newly weaned pigs at 6.2 kg ± 0.4 body weight (BW) were allotted to three dietary treatments based on a randomized complete block design with sex and initial BW as blocks. Three dietary treatments are as follows: Control, typical nursery feeds including animal protein supplements and enzyme-treated soybean meal; LCF with increased amounts of soybean meal, decreased amounts of animal protein supplements, and no enzyme-treated soybean meal; LCF+, low-cost formulated feed with β-mannanase at 100 g/t, providing 800 thermostable β-mannanase unit (TMU) per kg of feed. Pigs were fed based on a three-phase feeding program for a total of 37 d. On day 37 of feeding, all pigs were euthanized and the gastrointestinal tract was removed for sample collection to analyze intestinal health parameters, mucosa-associated microbiota, and gene expression of tight junction proteins. Pigs fed LCF increased (P < 0.05) the relative abundance of Proteobacteria and Helicobacter in the jejunal mucosa, tended to decrease (P = 0.097; P = 0.098) the concentration of malondialdehyde (MDA) and the expression of zona occluden 1 (ZO-1) gene in the jejunum, tended to decrease average daily gain (ADG; P = 0.084) and final BW (P = 0.090), and decreased (P < 0.05) average daily feed intake. Pigs fed LCF + tended to decrease (P = 0.088) digesta viscosity, decreased (P < 0.05) the relative abundance of Helicobacter, and increased (P < 0.05) Lactobacillus in the jejunal mucosa compared to LCF. Additionally, LCF + tended to increase final BW (P = 0.059) and ADG (P = 0.054), increased (P < 0.05) gain to feed ratio (G:F), and reduced (P < 0.05) fecal score compared to LCF. LCF with decreased amounts of animal protein supplements and increased amounts of soybean meal had negative effects on the composition of the mucosa-associated microbiota, intestinal integrity, and growth performance of nursery pigs. Beta-mannanase supplementation to LCF decreased digesta viscosity, increased the relative abundance of potentially health-benefitting microbiota such as Lactobacillus, and improved growth and fecal score, thus reflecting its efficacy in low-cost formulated feeds with increased amounts of soybean meal.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Baker, Jonathan T. and Deng, Zixiao and Sokale, Adebayo and Frederick, Brent and Kim, Sung Woo}, year={2024}, month={Aug} }
 @article{baker_duarte_kim_gormley_jang_2023, title={Effects of Increased Nsp with Or Without Nspase Supplementation on Growth Performance, Intestinal Health, and Intestinal Microbiome of Nursery Pigs and Implications on Subsequent Phases of Production}, volume={101}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skad281.233}, abstractNote={Abstract
               This study aimed to investigate the effects of increased NSP from alternative feedstuffs in feeds with or without NSPase supplementation on growth performance, nutrient digestibility, intestinal health, immune status, and modulation of the intestinal microbiome of nursery pigs and the carryover effect from dietary nursery intervention on the subsequent phases of production. One hundred and sixty newly weaned pigs at 7.0 ± 0.3 kg BW were allotted in a 2 x 2 factorial arrangement with NSP level and NSPase supplementation serving as factors and sex as block. Pigs were assigned to one of 4 dietary treatments with the control having corn DDGS as the main source of NSP and HNSP having soy hulls and wheat bran replacing corn DDGS as the source of NSP. Pigs were fed for 37 d in 2 phases (19 d and 18 d, respectively) and housed in a pen of 4. Growth performance was measured at the end of each phase. At d 37, a pig representing the median BW within each pen from each treatment was euthanized for sampling jejunal mucosa, jejunal tissue, and ileal digesta to analyze intestinal health variables, oxidative stress products, and digesta viscosity. The remaining pigs were then fed a common diet for the subsequent grower and finisher phases to evaluate the carryover effect. At the end of the finisher phase, all pigs were euthanized to obtain carcass data. Data were analyzed using Mixed procedure of SAS 9.4. Overall, NSP level and type nor NSPase supplementation had an effect on growth performance; however, HNSP tended to have improved (P = 0.087) feed efficiency (0.52 to 0.53) in the grower period immediately following the nursery phase. Additionally, HNSP increased (P < 0.05) IgG (4.84 to 5.83 µg/mg of protein) in the jejunal mucosa and tended to increase (P = 0.096) the villus height in the jejunum (482.8 to 506.6 μm). The body weight of HNSP pigs was more uniform (P < 0.05; 8.7 to 11.6%) than the control at the end of the 120-day trial. No differences were observed in carcass traits however, the hot carcass weight of pigs in HNSP tended to be more uniform (P = 0.089; 6.4 to 10.00%) than the control. In conclusion, increased amounts of NSP from alternative feedstuffs does not further impair or damage the intestinal health and subsequent growth performance of nursery pigs and may aid the pig through generated metabolites and alterations to the intestinal microbiota as they are transitioned to the ensuing phases of production as evidenced by the increased uniformity at the end of the trial.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Baker, Jonathan T. and Duarte, Marcos Elias Elias and Kim, Sung Woo and Gormley, Alexa R. and Jang, Ki beom}, year={2023}, month={Nov}, pages={192–193} }
 @article{baker_duarte_kim_2023, title={Effects of Xylanase Supplementation in Diets with Reduced Metabolizable Energy and Supplemental Fat on Intestinal Health, Mucosa Associated Microbiota and Growth Performance of Nursery Pigs}, volume={101}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skad281.234}, abstractNote={Abstract
               This study aimed to investigate the effects of reducing metabolizable energy (ME) below the requirement by reducing supplemental fat on intestinal health and growth performance of nursery pigs. One hundred and ninety-two newly weaned pigs at 7.3 ± 0.6 kg BW were allotted in a RCBD with sex and initial BW as blocks. Pigs were assigned to one of 8 dietary treatments with varying ME levels (3,400, 3,375, 3,350, 3,325, and 3,300 kcal/kg ME) by reducing supplemental fat and increasing xylanase (X0: 0, X40: 1,200, X80: 2,400 and X120: 3,600 XU/kg of feed) in ME deficient diets by 100 kcals/kg. Pigs were fed diets for 35 d in 2 phases (14 d and 21 d, respectively) and growth performance was measured at the end of each phase. Phase 2 diet had titanium dioxide (0.4%) as indigestible external marker to measure ileal digestibility of nutrients. At d 35, a pig representing the median BW within each pen from 3 dietary treatments (ME at 3,400 and 3,300 kcal/kg ME with or without xylanase) were euthanized for sampling jejunal mucosa, jejunal tissue, and ileal digesta to analyze intestinal health parameters, mucosa associated microbiota, and apparent ileal digestibility (AID) of nutrients. Data were analyzed using Mixed procedure of SAS 9.4. Overall, reducing ME level had no effect on growth performance of nursery pigs. However, reducing ME by 100 kcal/kg ME below the requirement increased (P < 0.05) digesta viscosity (1.7 to 2.2 cP) and protein carbonyl (0.18 to 0.40 nmol/mg), tended to change the relative abundance of Prevotellaceae (P = 0.062, 49 to 23%) and Lactobacillaceae (P = 0.086, 7 to 19%), reduced (P < 0.05) villus height (467 to 433 µm) and villus height to crypt depth ratio (1.80 to 1.64) in the jejunum, and reduced (P < 0.05) AID of DM (59.5 to 47.3%), gross energy (59.1 to 43.0%), crude protein (63.4 to 44.6%), and ether extract (67.7 to 55.2%). Xylanase supplementation decreased (P < 0.05) digesta viscosity (2.2 to 1.82 cP) and protein carbonyl (0.40 to 0.17 nmol/mg), increased the relative abundance of Prevotellaceae (20.22 to 61.56%), increased villus height (433 to 462 µm) and villus height to crypt depth ratio (1.64 to 1.99) in the jejunum, and increased (P < 0.05) AID of DM (47.3 to 58.7%), and gross energy (42.9 to 56.0%). In conclusion, reducing ME (100 kcal/kg below the requirement) by reducing supplemental fat (by 2% unit) impaired intestinal health, reducing ability of the pigs to digest feeds whereas xylanase supplementation appeared to mediate some of the negative effects seen by energy reduction by improving intestinal morphology, reducing oxidative damage products, and diversity of the mucosa-associated microbiota.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Baker, Jonathan T. and Duarte, Marcos Elias Elias and Kim, Sung Woo}, year={2023}, month={Nov} }
 @article{baker_duarte_kim_2022, title={Effects of Reduced Metabolizable Energy by Reducing fat Supplementation on Growth Performance and Intestinal Health of Nursery Pigs}, volume={100}, ISSN={["1525-3163"]}, url={https://publons.com/wos-op/publon/61555372/}, DOI={10.1093/jas/skac247.230}, abstractNote={Abstract
               This study aimed to investigate the effects of reducing metabolizable energy (ME) by reducing fat supplementation on growth performance and intestinal health of nursery pigs. Nursery pigs (n = 120; 7.3 ± 0.6 kg BW) were allotted in a randomized complete block design with sex and initial BW as blocks. Pigs were assigned to 5 dietary treatments with decreasing ME levels (3,400, 3,375, 3,350, 3,325, and 3,300 kcal/kg ME). ME content was gradually reduced by reducing fat supplementation (2%). Pigs were fed for 35 d in 2 phases (P1: 14 d and P2: 21 d). Titanium dioxide was added (0.4%) to P2 diets as an indigestible external marker to measure apparent ileal digestibility (AID) of nutrients. At d 35, a pig with median BW of each pen in 2 treatments (3,400 and 3,300 kcal/kg) was euthanized for sampling jejunal mucosa to analyze oxidative stress and immune status. Jejunal digesta was collected to measure viscosity. Ileal digesta was collected to analyze AID of nutrients. Data were analyzed using SAS. Reducing ME level by 3% (100 kcal/kg) had no effect on growth performance. The 3,300 kcal/kg diet reduced (P < 0.05) AID of DM (59.5 to 47.3%), GE (59.1 to 43.0%), CP (63.4 to 44.6%), and EE (67.7 to 55.2%), increased (P < 0.05) digesta viscosity (1.7 to 2.2 cP) and VH:CD (1.64 to 1.80), and decreased (P < 0.05) VH (467 to 433 µm) when compared with 3,400 kcal/kg diet. Additionally, the 3,300 kcal/kg diet increased (P < 0.05) the concentration of protein carbonyl (0.18 to 0.40 nmol/mg) in the jejunal mucosa. In conclusion, reducing ME by reducing fat supplementation by 2% increased the viscosity of jejunal digesta and protein carbonyl in the jejunal mucosa, resulting in decreased villus height and nutrient digestibility, without affecting growth performance.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Baker, Jonathan and Duarte, Marcos Elias E. and Kim, Sung Woo}, year={2022}, month={Oct}, pages={120–120} }
 @article{baker_kim_2022, title={Effects of non-Starch Polysaccharide Degrading Enzymes plus Lysophospholipids in Feeds with Varied ME Contents on Growth Performance and Nutrient Utilization of Pigs from 25 to 75 Kg BW}, volume={100}, ISSN={["1525-3163"]}, url={https://publons.com/wos-op/publon/61555369/}, DOI={10.1093/jas/skac247.223}, abstractNote={Abstract
               This study aimed to investigate efficacy of the combinational use of non-starch polysaccharide degrading enzymes (NSPase) and lysophospholipids (LPL) on growth performance and nutrient utilization in pigs fed feeds with varied metabolizable energy (ME) levels. Grower pigs (n = 270; 28.1 ± 0.6 kg BW) were allotted in a randomized complete block design with a 3 x 3 factorial arrangement of treatments fed for 51 d in 2 phases (30 and 21 d, respectively). Two factors were ME (3,260, 3,335, and 3,410 kcal/kg) and NSPase + LPL (ELPL at 0.00, 0.05, and 0.10%). Growth performance and backfat were measured. Blood was collected to measure plasma urea nitrogen (PUN) concentrations. Data were analyzed using the MIXED procedure on SAS 9.4. Increasing ME tended to increase (P = 0.066) ADG during Grower 2 and increased (P < 0.05) G/F during the overall period. Increasing ELPL supplementation increased (P < 0.05) both ADG and ADFI during Grower 2, whereas it tended to reduce (P = 0.084) G/F during the overall period. Supplemental effects of ELPL on increasing ADG was prominent (P < 0.05) at high ME content (3% above NRC requirement). Supplementation of ELPL tended to reduce (P = 0. 057) G/F during Grower 2 at low ME content (3% below NRC requirements). Backfat thickness and NEFA were not influenced by 2 factors. Increasing ME increased (P < 0.05) PUN during Grower 1 whereas increasing ELPL tended to increase (P = 0.077) PUN during Grower 2. In conclusion, supplementation of ELPL enhanced growth and feed intake of pigs from 50 to 75 kg BW, especially when dietary ME was above NRC requirement (3%) by fat supplementation (4%). These improvements may be due to increased nutrient utilization as indirectly indicated by increased PUN during fed status.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Baker, Jonathan and Kim, Sung Woo}, year={2022}, month={Oct}, pages={116–116} }
 @article{duarte_deng_baker_kim_2022, title={Impact of Reducing Dietary Protein with Supplementation of 8 Essential Amino Acids and Functional Roles of Supplemental his or Glu on Growth Performance and Carcass Traits of Finishing Pigs.}, volume={100}, ISSN={["1525-3163"]}, url={https://publons.com/wos-op/publon/61555367/}, DOI={10.1093/jas/skac247.216}, abstractNote={Abstract
               This study evaluated the effects of reducing dietary protein with supplementation of 8 essential amino acids and functional roles of supplemental Glu or His on growth performance and carcass traits of finishing pigs. Pigs (n = 216; 98 ± 3.5 kg BW) were allotted (3 pigs/pen) to 4 treatments in a randomized complete block design (RCBD) with sex and initial BW as blocks. Pigs were fed for 14 d until 120 kg BW. Dietary treatments were CON (11.4% CP with L-Lys, L-Thr, and L-Trp); LCP (8.9% CP with L-Lys, Thr, Trp, Met, Val, Ile, Phe, and His); LCPG (9.1% CP; LCP+Glu to match % Glu in CON); and LCPH (9.2% CP; LCP+2x His requirement). Diets met or exceeded requirements with 2.7 Mcal/kg NE. Growth performance and backfat thickness (BFT) were measured at d 0 and d 14. Blood was collected to analyze BUN. At d 14, pigs with the median BW in each pen were slaughtered. Pigs fed CON had greatest (P < 0.05) BW, ADG, and G:F. Pigs fed LCP had greater (P < 0.05) BW, ADG, and ADFI than LCPG. BFT of pigs fed LCPG and LCPH was less (P < 0.05) than CON. Cold carcass weight in CON was greater (P < 0.05) than LCPG and LCPH, whereas it was greater (P < 0.05) in LCP than in LCPG. Chilling loss in LCP was less (P < 0.05) than LCPG. Drip loss at 48 h in LCPH was less (P < 0.05) than CON and LCPG. BUN in LCPG and LCPH was less (P < 0.05) than CON. In conclusion, reducing dietary protein by 2.5% using 8 supplemental amino acids reduced growth performance without affecting carcass yield. Supplemental His at 2x requirement to low protein diets meeting His requirement enhanced loin quality by reducing drip loss.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Duarte, Marcos Elias E. and Deng, Zixiao and Baker, Jonathan and Kim, Sung Woo}, year={2022}, month={Oct}, pages={112–112} }