@article{verma_lin_smith_walker_hewezi_davis_hussey_baum_mitchum_2022, title={A novel sugar beet cyst nematode effector 2D01 targets the Arabidopsis HAESA receptor-like kinase}, ISSN={["1364-3703"]}, DOI={10.1111/mpp.13263}, abstractNote={Abstract Plant‐parasitic cyst nematodes use a stylet to deliver effector proteins produced in oesophageal gland cells into root cells to cause disease in plants. These effectors are deployed to modulate plant defence responses and developmental programmes for the formation of a specialized feeding site called a syncytium. The Hg2D01 effector gene, coding for a novel 185‐amino‐acid secreted protein, was previously shown to be up‐regulated in the dorsal gland of parasitic juveniles of the soybean cyst nematode Heterodera glycines , but its function has remained unknown. Genome analyses revealed that Hg2D01 belongs to a highly diversified effector gene family in the genomes of H. glycines and the sugar beet cyst nematode Heterodera schachtii . For functional studies using the model Arabidopsis thaliana – H. schachtii pathosystem, we cloned the orthologous Hs2D01 sequence from H. schachtii . We demonstrate that Hs2D01 is a cytoplasmic effector that interacts with the intracellular kinase domain of HAESA (HAE), a cell surface‐associated leucine‐rich repeat (LRR) receptor‐like kinase (RLK) involved in signalling the activation of cell wall‐remodelling enzymes important for cell separation during abscission and lateral root emergence. Furthermore, we show that AtHAE is expressed in the syncytium and, therefore, could serve as a viable host target for Hs2D01. Infective juveniles effectively penetrated the roots of HAE and HAESA‐LIKE2 ( HSL2 ) double mutant plants; however, fewer nematodes developed on the roots, consistent with a role for this receptor family in nematode infection. Taken together, our results suggest that the Hs2D01–AtHAE interaction may play an important role in sugar beet cyst nematode parasitism.}, journal={MOLECULAR PLANT PATHOLOGY}, author={Verma, Anju and Lin, Marriam and Smith, Dante and Walker, John C. and Hewezi, Tarek and Davis, Eric L. and Hussey, Richard S. and Baum, Thomas J. and Mitchum, Melissa G.}, year={2022}, month={Sep} }
 @article{chen_yang_xia_bowman_williams_walker_shi_2018, title={The extent and pathways of nitrogen loss in turfgrass systems: Age impacts}, volume={637}, ISSN={["1879-1026"]}, DOI={10.1016/j.scitotenv.2018.05.053}, abstractNote={Nitrogen loss from fertilized turf has been a concern for decades, with most research focused on inorganic (NO3−) leaching. The present work examined both inorganic and organic N species in leachate and soil N2O emissions from intact soil cores of a bermudagrass chronosequence (1, 15, 20, and 109 years old) collected in both winter and summer. Measurements of soil N2O emissions were made daily for 3 weeks, while leachate was sampled once a week. Four treatments were established to examine the impacts of fertilization and temperature: no N, low N at 30 kg N ha−1, and high N at 60 kg N ha−1, plus a combination of high N and temperature (13 °C in winter or 33 °C in summer compared to the standard 23 °C). Total reactive N loss generally showed a “cup” pattern of turf age, being lowest for the 20 years old. Averaged across all intact soil cores sampled in winter and summer, organic N leaching accounted for 51% of total reactive N loss, followed by inorganic N leaching at 41% and N2O-N efflux at 8%. Proportional loss among the fractions varied with grass age, season, and temperature and fertilization treatments. While high temperature enhanced total reactive N loss, it had little influence on the partitioning of loss among dissolved organic N, inorganic N and N2O-N when C availability was expected to be high in summer due to rhizodeposition and root turnover. This effect of temperature was perhaps due to higher microbial turnover in response to increased C availability in summer. However when C availability was low in winter, warming might mainly affect microbial growth efficiency and therefore partitioning of N. This work provides a new insight into the interactive controls of warming and substrate availability on dissolved organic N loss from turfgrass systems.}, journal={SCIENCE OF THE TOTAL ENVIRONMENT}, author={Chen, Huaihai and Yang, Tianyou and Xia, Qing and Bowman, Daniel and Williams, David and Walker, John T. and Shi, Wei}, year={2018}, month={Oct}, pages={746–757} }