@article{mwanga_yencho_gibson_moyer_2013, title={Methodology for Inoculating Sweetpotato Virus Disease: Discovery of Tip Dieback, and Plant Recovery and Reversion in Different Clones}, volume={97}, ISSN={["1943-7692"]}, DOI={10.1094/pdis-12-11-1072-re}, abstractNote={Evaluating sweetpotato (Ipomoea batatas) genotypes for resistance to sweetpotato virus disease (SPVD) has been slow and inefficient. Ipomoea setosa plants, normally used as the source of scions for graft-infecting sweetpotatoes with viral diseases, are often severely stunted and their mortality is 10 to 30% when infected with SPVD, making them unsuitable as scions. Tanzania, a landrace of I. batatas widely grown in East Africa, was found to be a superior host for maintaining and increasing SPVD inoculum (scions) for mass grafting. Modifications to a cleft-grafting technique also increased survival of grafted SPVD-affected scions from 5 to 100%. These modifications, coupled with an efficient SPVD scoring technique, allowed rapid screening of large sweetpotato populations for SPVD resistance. Plant recovery from SPVD is reported here as a component of SPVD resistance. Differences in recovery from SPVD were detected among progenies, indicating its genetic basis. Plant tip dieback, a hypersensitivity response, was observed only in families with cv. Wagabolige as a parent. These findings may open up new opportunities for improved understanding and control of this devastating disease.}, number={1}, journal={PLANT DISEASE}, publisher={Scientific Societies}, author={Mwanga, R. O. M. and Yencho, G. C. and Gibson, R. W. and Moyer, J. W.}, year={2013}, month={Jan}, pages={30–36} } @article{sullivan_parks_cubeta_gallup_melton_moyer_shew_2010, title={An Assessment of the Genetic Diversity in a Field Population of Phytophthora nicotianae with a Changing Race Structure}, volume={94}, ISSN={["1943-7692"]}, DOI={10.1094/pdis-94-4-0455}, abstractNote={ One hundred fifty-three isolates of Phytophthora nicotianae that were collected over a 4-year period from a single field were subjected to amplified fragment length polymorphism (AFLP) analysis to investigate the effect of different types of resistance in tobacco (Nicotiana tabacum) on genetic diversity in the pathogen population. No race 1 isolates were detected in the field prior to initiating the study, but the race was present in multiple plots by the end of the 4-year period. There were 102 race 0 isolates and 51 race 1 isolates characterized. Seventy-six of the 153 isolates had a unique AFLP profile, whereas the remaining 77 isolates were represented by 27 AFLP profiles shared by at least two isolates. Isolates of both races were found in both the unique and shared AFLP profile groups. Twenty-three of the AFLP profiles were detected in multiple years, indicating a clonal component to the pathogen population. Race 1 isolates that were detected over multiple years were always obtained from the same plot. No race 1 profile was found in more than one plot, confirming the hypothesis that the multiple occurrences of the race throughout the field were the result of independent events and not pathogen spread. Three identical race 0 AFLP profiles occurred in noncontiguous plots, and in each case, the plots contained the same partially resistant variety. Cluster analysis provided a high level of bootstrap support for 41 isolates in 19 clusters that grouped primarily by race and rotation treatment. Estimates of genetic diversity ranged from 0.365 to 0.831 and varied depending on tobacco cultivar planted and race. When averaged over all treatments, diversity in race 1 isolates was lower than in race 0 isolates at the end of each season. Deployment of single-gene resistance initially decreased genetic diversity of the population, but the diversity increased each year, indicating the pathogen was adapting to the host genotypes deployed in the field. }, number={4}, journal={PLANT DISEASE}, author={Sullivan, M. J. and Parks, E. J. and Cubeta, M. A. and Gallup, C. A. and Melton, T. A. and Moyer, J. W. and Shew, H. D.}, year={2010}, month={Apr}, pages={455–460} } @article{kaye_moyer_parks_carbone_cubeta_2011, title={Population Genetic Analysis of Tomato spotted wilt virus on Peanut in North Carolina and Virginia}, volume={101}, ISSN={["1943-7684"]}, DOI={10.1094/phyto-01-10-0035}, abstractNote={ Exploring the genetic diversity and evolutionary history of plant viruses is critical to understanding their ecology and epidemiology. In this study, maximum-likelihood and population genetics-based methods were used to investigate the population structure, genetic diversity, and sources of genetic variation in field isolates of Tomato spotted wilt virus (TSWV) from peanut in North Carolina and Virginia. Selected regions of the nucleocapsid, movement, and RNA-dependent RNA polymerase genes were amplified and sequenced to identify haplotypes and infer genetic relationships between isolates of TSWV with heuristic methods. The haplotype structure of each locus consisted of 1 or 2 predominant haplotypes and >100 haplotypes represented by a single isolate. No specific haplotypes were associated with geographic area, peanut cultivar, or year of isolation. The population was panmictic at the regional level and high levels of genetic diversity were observed among isolates. There was evidence for positive selection on single amino acids in each gene on a background of predominant purifying selection acting upon each locus. The results of compatibility analyses and the persistence of specific gene sequences in isolates collected over three field seasons suggest that recombination was occurring in the population. Estimates of the population mutation rate suggest that mutation has had a significant effect on the shaping of this population and, together with purifying selection, these forces have been the predominant evolutionary forces influencing the TSWV population in peanut in North Carolina and Virginia. }, number={1}, journal={PHYTOPATHOLOGY}, author={Kaye, A. C. and Moyer, J. W. and Parks, E. J. and Carbone, I. and Cubeta, M. A.}, year={2011}, month={Jan}, pages={147–153} } @article{macdonald_allen_gadoury_jacobi_kelemu_moyer_murray_ong_pearson_sherwood_et al._2009, title={Education in Plant Pathology Present Status and Future Challenges}, volume={93}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS-93-12-1238}, abstractNote={HomePlant DiseaseVol. 93, No. 12Education in Plant Pathology: Present Status and Future Challenges PreviousNext Education in Plant Pathology: Present Status and Future ChallengesJames MacDonald, Caitilyn Allen, David Gadoury, William Jacobi, Segenet Kelemu, James Moyer, Tim Murray, Kevin Ong, Charles Pearson, John Sherwood, and Ann VidaverJames MacDonaldCorresponding author: James D. MacDonald, Department of Plant Pathology, University of California, Davis; E-mail: E-mail Address: [email protected], Caitilyn Allen, David Gadoury, William Jacobi, Segenet Kelemu, James Moyer, Tim Murray, Kevin Ong, Charles Pearson, John Sherwood, and Ann VidaverAffiliationsAuthors and Affiliations James MacDonald , University of California, Davis Caitilyn Allen , University of Wisconsin, Madison David Gadoury , Cornell University, Geneva, NY William Jacobi , Colorado State University, Fort Collins Segenet Kelemu , International Livestock Research Institute, Nairobi, Kenya James Moyer , North Carolina State University, Raleigh Tim Murray , Washington State University, Pullman Kevin Ong , Texas Plant Disease Diagnostic Lab, Texas A&M University, College Station Charles Pearson , Syngenta Crop Protection, Greensboro, NC John Sherwood , University of Georgia, Athens Ann Vidaver , University of Nebraska, Lincoln Published Online:5 Nov 2009https://doi.org/10.1094/PDIS-93-12-1238AboutSectionsPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat DetailsFiguresLiterature CitedRelated Vol. 93, No. 12 December 2009SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 5 Nov 2009Published: 5 Nov 2009 Pages: 1238-1251 Information© 2009 The American Phytopathological SocietyPDF downloadCited byThe Need and a Vision for a Diagnostic Assay Validation NetworkKitty F. 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Nakhla1 March 2023 | PhytoFrontiers™, Vol. 0, No. 0Addressing biohazards to food security in primary production2 July 2022 | Food Security, Vol. 14, No. 6Plant pathology in the era of new education policy: challenges and opportunities19 July 2021 | Indian Phytopathology, Vol. 74, No. 3A fresh look at graduate education in Plant Pathology in a changing world: global needs and perspectives9 March 2020 | Journal of Plant Pathology, Vol. 102, No. 3Finding the Gaps: An Assessment of Concepts, Skills, and Employer Expectations for Plant Pathology Foundational CoursesBrantlee Spakes Richter, Anissa Poleatewich, Maya Hayslett, and Kathryn Stofer30 August 2018 | Plant Disease, Vol. 102, No. 10Forest Health in a Changing World13 December 2014 | Microbial Ecology, Vol. 69, No. 4Networks in Plant Epidemiology: From Genes to Landscapes, Countries, and ContinentsMathieu Moslonka-Lefebvre, Ann Finley, Ilaria Dorigatti, Katharina Dehnen-Schmutz, Tom Harwood, Michael J. Jeger, Xiangming Xu, Ottmar Holdenrieder, and Marco Pautasso10 March 2011 | Phytopathology®, Vol. 101, No. 4Plant Pathology: A Story About BiologyAnnual Review of Phytopathology, Vol. 48, No. 1Where will the next Norman Borlaug come from? A U.S. perspective of plant pathology education and research.Plant Protection Science, Vol. 45, No. 4}, number={12}, journal={PLANT DISEASE}, author={MacDonald, James and Allen, Caitilyn and Gadoury, David and Jacobi, William and Kelemu, Segenet and Moyer, James and Murray, Tim and Ong, Kevin and Pearson, Charles and Sherwood, John and et al.}, year={2009}, month={Dec}, pages={1238–1251} } @article{abad_parks_new_fuentes_jester_moyer_2007, title={First report of Sweet potato chlorotic stunt virus, a component of sweetpotato virus disease, in North Carolina.}, volume={91}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS-91-3-0327B}, abstractNote={ Sweet potato chlorotic stunt virus (SPCSV) is the whitefly-transmitted component of the sweet potato virus disease (SPVD), a devastating disease originally described in Africa (4). Two isolates designated as G-01 and T-03 were obtained in North Carolina in July 2001 and October 2003, respectively, from plants of cv. Beauregard exhibiting symptoms typical of SPVD, including stunting, leaf narrowing and distortion, vein clearing, and chlorotic mosaic. Sap extract from symptomatic plants tested positive for SPCSV by nitrocellulose immuno-dot blot, using monoclonal antibodies specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from 100 mg of symptomatic leaf tissue by using the PureLink Total RNA Purification System Kit from Invitrogen (Carlsbad, CA) with a minor modification (adding 2% PVP-40 and 1% 2-mercaptoethanol to the extraction buffer) (1). Results were confirmed by reverse transcription (RT)-PCR using primers CP1 and CP3 and HSP70-A/HSP70-B (2), corresponding to the capsid protein and ‘heat shock’ protein genes, respectively. HSP70 amplicons were cloned using the TOPO TA Cloning Kit (Invitrogen) and sequenced. At the nucleotide level, viral sequences from clones from both isolates were an average 99.4% similar to West Africa and 77.9% to East Africa sequences of SPCSV from Genbank (1). Although the isolates were collected from different fields, viral sequences generated from clones for T-03 and G-01 differed by only six nucleotides and were identical at the amino acid level. The neighbor-joining phylogenetic tree constructed using the HSP70 gene fragment (39 nt) delineated two major clusters with two subpopulations each: Cluster 1, “East Africa”, consisted of East Africa and Peru subpopulations; Cluster 2, “West Africa”, consisted of Argentina-Brazil and USA-West Africa subpopulations (1). In addition, SPCSV isolates from East Africa and West Africa clusters were sufficiently distant phylogenetically to suggest that they may correspond to two different criniviruses, with an average similarity between the populations of 78.14% and an average within the populations above 89%. Hudson's tests confirmed the presence of genetically distinct SPCSV groups with high statistical significance (1). Two groups (Peru and East Africa) were differentiated in the East Africa cluster, and three groups (Argentina-Brazil, USA, and West Africa) were differentiated in the West Africa cluster, suggesting that the USA population is not a recent introduction. Although SPCSV was previously reported in the United States, the source was a single accession of cv. White Bunch from the USDA Sweetpotato Germplasm Repository (3). Sweet potato feathery mottle virus (SPFMV) (family Potyviridae, genus Potyvirus), the other component of SPVD, was also detected in both cultivars. To our knowledge, this is the first report of SPCSV in sweetpotato fields in the United States. References: (1) J. A. Abad et al. Phytopathology (Abstr.) 96(suppl.):S1, 2006. (2) T. Alicai et al. Plant Pathol. 48:718, 1999. (3) G. Pio-Ribeiro et al. Plant Dis. 80:551, 1996. (4) G. A. Schaefer and E. R. Terry. Phytopathology 66:642, 1977. }, number={3}, journal={PLANT DISEASE}, author={Abad, J. A. and Parks, E. J. and New, S. L. and Fuentes, S. and Jester, W. and Moyer, J. W.}, year={2007}, month={Mar}, pages={327–327} } @article{parks_moyer_lyerly_2006, title={Identification of fluorescent AFLP and SSR markers for differentiation and analysis of New Guinea impatiens}, volume={131}, number={5}, journal={Journal of the American Society for Horticultural Science}, author={Parks, E. J. and Moyer, J. W. and Lyerly, J. H.}, year={2006}, pages={622–631} } @article{abad_moyer_kennedy_holmes_cubeta_2005, title={Tomato spotted wilt virus on potato in eastern North Carolina}, volume={82}, ISSN={["1874-9380"]}, DOI={10.1007/BF02853592}, number={3}, journal={AMERICAN JOURNAL OF POTATO RESEARCH}, author={Abad, JA and Moyer, JW and Kennedy, GG and Holmes, GA and Cubeta, MA}, year={2005}, pages={255–261} } @article{sin_mcnulty_kennedy_moyer_2005, title={Viral genetic determinants for thrips transmission of Tomato spotted wilt virus}, volume={102}, ISSN={["0027-8424"]}, DOI={10.1073/pnas.0407354102}, abstractNote={ Tomato spotted wilt virus (TSWV) is transmitted exclusively by thrips in nature. A reassortment-based viral genetic system was used to map transmissibility by thrips to the medium (M) RNA of TSWV. To locate determinants of thrips transmission in the M RNA, 30 single-lesion isolates (SLIs) were generated from a single TSWV isolate that was inefficiently transmitted by thrips. Three of the 30 SLIs were transmitted by thrips, and 27 were not. Sequence analysis of the M RNA, thrips transmissibility assays, G C protein analysis, and transmission electron microscopic studies revealed that a specific nonsynonymous mutation (C1375A) in the G N /G C ORF of the M RNA resulted in the loss of thrips transmissibility without inhibition of virion assembly. This was in contrast to other nontransmissible SLIs, which had frameshift and/or nonsense mutations in the G N /G C ORF but were defective in virion assembly. The G C glycoprotein was detectable in the C1375A mutants but not in the frameshift or nonsense mutants. We report a specific viral determinant associated with virus transmission by thrips. In addition, the loss of transmissibility was associated with the accumulation of defective haplotypes in the population, which are not transmissible by thrips, rather than with the presence of a dominant haplotype that is inefficiently transmitted by thrips. These results also indicate that the glycoproteins may not be required for TSWV infection of plant hosts but are required for transmissibility by thrips. }, number={14}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Sin, SH and McNulty, BC and Kennedy, GG and Moyer, JW}, year={2005}, month={Apr}, pages={5168–5173} } @article{parks_moyer_2004, title={Evaluation of AFLP in poinsettia: Polymorphism selection, analysis, and cultivar identification}, volume={129}, ISSN={["0003-1062"]}, DOI={10.21273/jashs.129.6.0863}, abstractNote={Fingerprinting using molecular markers is a highly effective method of cultivar identification that is a powerful aid to traditional methods based on morphology. Amplified fragment length polymorphism (AFLP) is a robust and reliable method for generating molecular markers that has been used to evaluate many crops for a variety of applications. In this study, AFLP was used to develop and validate robust genetic fingerprints for poinsettia (Euphorbia pulcherrima Willd. ex Klotzch) cultivars. Polymorphism selection was completed to facilitate the identification of useful polymorphisms and minimize future fingerprinting costs and time. Poinsettia is a highly variable crop subject to genetic drift and variable cultivars. Validation of polymorphisms to remove those associated with intracultivar variation improved the reliability of the fingerprinting. The result was a poinsettia AFLP database that defines the genetic fingerprints of 104 cultivars. Cluster analysis illustrated differentiation of most poinsettia cultivars tested. Selection of a subset of AFLP polymorphisms resulted in clustering of cultivars according to known origin and breeding program. This method has applications not only for cultivar identification for cultivar protection, and maintenance of cultivar uniformity, but also has the potential application of developing markers for important traits.}, number={6}, journal={JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE}, author={Parks, EJ and Moyer, JW}, year={2004}, month={Nov}, pages={863–869} } @article{tsompana_abad_purugganan_moyer_2005, title={The molecular population genetics of the Tomato spotted wilt virus (TSWV) genome}, volume={14}, ISSN={["1365-294X"]}, DOI={10.1111/j.1365-294X.2004.02392.x}, abstractNote={AbstractRNA viruses are characterized by high genetic variability resulting in rapid adaptation to new or resistant hosts. Research for plant RNA virus genetic structure and its variability has been relatively scarce compared to abundant research done for human and animal RNA viruses. Here, we utilized a molecular population genetic framework to characterize the evolution of a highly pathogenic plant RNA virus [Tomato spotted wilt virus (TSWV), Tospovirus, Bunyaviridae]. Data from genes encoding five viral proteins were used for phylogenetic analysis, and for estimation of population parameters, subpopulation differentiation, recombination, divergence between Tospovirus species, and selective constraints on the TSWV genome. Our analysis has defined the geographical structure of TSWV, attributed possibly to founder effects. Also, we identify positive selection favouring divergence between Tospovirus species. At the species level, purifying selection has acted to preserve protein function, although certain amino acids appear to be under positive selection. This analysis provides demonstration of population structuring and species‐wide population expansions in a multisegmented plant RNA virus, using sequence‐based molecular population genetic analyses. It also identifies specific amino acid sites subject to selection within Bunyaviridae and estimates the level of genetic heterogeneity of a highly pathogenic plant RNA virus. The study of the variability of TSWV populations lays the foundation in the development of strategies for the control of other viral diseases in floral crops.}, number={1}, journal={MOLECULAR ECOLOGY}, author={Tsompana, M and Abad, J and Purugganan, M and Moyer, JW}, year={2005}, month={Jan}, pages={53–66} } @article{lyerly_new_abad_moyer_2003, title={Identification of sweetpotato viruses using an RT- PCR based method}, volume={93}, journal={Phytopathology}, author={Lyerly, J. H. and New, S. L. and Abad, J. A. and Moyer, J. W.}, year={2003}, pages={S55} } @article{groves_walgenbach_moyer_kennedy_2003, title={Seasonal dispersal patterns of Frankliniella fusca (Thysanoptera : Thripidae) and tomato spotted wilt virus occurrence in central and eastern North Carolina}, volume={96}, ISSN={["1938-291X"]}, DOI={10.1603/0022-0493-96.1.1}, abstractNote={Abstract The seasonal abundance and temporal pattern of Frankliniella fusca Hinds dispersal were monitored from 1996 to 2000 at 12 locations in central and eastern North Carolina. The predominant vector species of tomato spotted wilt virus (TSWV) captured across all locations was F. fusca (98%). The temporal patterns of F. fusca dispersal observed during spring seasons varied among locations in all years except 2000. Regression analysis estimated that times of first flight in the spring seasons varied among locations, whereas flight duration intervals were similar. Temporal patterns of F. fusca captured varied significantly between aerial traps placed 0.1 and 1.0 m above the soil surface. Fewer total thrips were captured at 0.1 m, although thrips dispersal occurred earlier and over a greater time interval compared with 1.0-m traps. Temporal patterns of TSWV occurrence differed among locations in the spring seasons of 1999 and 2000, whereas patterns of virus occurrence were similar during the fall seasons. Patterns of F. fusca dispersal and subsequent TSWV occurrence were synchronous at locations in 1999 and 2000 where the greatest number of TSWV lesions was recorded. Knowledge of the temporal patterns of F. fusca dispersal and TSWV occurrence may be a useful indicator for describing the time when susceptible crops are at highest risk of TSWV infection.}, number={1}, journal={JOURNAL OF ECONOMIC ENTOMOLOGY}, author={Groves, RL and Walgenbach, JF and Moyer, JW and Kennedy, GG}, year={2003}, month={Feb}, pages={1–11} } @article{mwanga_yencho_moyer_2002, title={Diallel analysis of sweetpotatoes for resistance to sweetpotato virus disease}, volume={128}, ISSN={["0014-2336"]}, DOI={10.1023/A:1020828421757}, number={2}, journal={EUPHYTICA}, author={Mwanga, ROM and Yencho, CGC and Moyer, JW}, year={2002}, pages={237–248} } @article{lyerly_stalker_moyer_hoffman_2002, title={Evaluation of Arachis species for resistance to tomato spotted wilt virus}, volume={29}, DOI={10.3146/pnut.29.2.0001}, abstractNote={Abstract Tomato spotted wilt virus (TSWV) is an important plant pathogen with a wide host range, including the domesticated peanut (Arachis hypogaea L.). After initial outbreaks on peanut during the 1980s, the virus has spread to all peanut-producing states in the U.S. TSWV is transmitted by several species of thrips which are difficult to control with insecticides; therefore, control of TSWV most likely will come from selecting resistant genotypes in breeding programs. Although moderate levels of resistance have been discovered in A. hypogaea, complete virus resistance has not been found. Several Arachis species have desirable genes for plant resistances and tolerate many disease and insect pests better than the cultivated species. The objectives of this study were to (a) evaluate TSWV disease incidence and severity in accessions of Arachis species, and (b) compare levels of TSWV resistance in diploid species to selected A. hypogaea genotypes. In this study, 46 diploid Arachis spp. accessions were evaluated in the greenhouse by artificial inoculation tests for resistance to TSWV. Nine Arachis accessions were observed with no disease symptoms when TSWV isolate 10 was used as opposed to A. hypogaea lines that ranged from moderately to highly susceptible. Additional testing with more virulent isolates identified A. diogoi accession GKP 10602 and A. correntina accession GKP 9530 as highly resistant to the virus. These two accessions are being used as parents in crossing programs to incorporate TSWV resistance genes into A. hypogaea.}, journal={Peanut Science}, author={Lyerly, J. H. and Stalker, H. T. and Moyer, J. W. and Hoffman, K.}, year={2002}, pages={79–84} } @article{moyer_abad_new_bell_2002, title={Isolation, identification and detection of undescribed RNA sweetpotato viruses}, ISBN={["90-6605-985-0"]}, ISSN={["0567-7572"]}, DOI={10.17660/actahortic.2002.583.13}, number={583}, journal={PROCEEDINGS OF THE FIRST INTERNATIONAL CONFERENCE ON SWEETPOTATO: FOOD AND HEALTH FOR THE FUTURE}, author={Moyer, JW and Abad, JA and New, J and Bell, J}, year={2002}, pages={121–127} } @article{mwanga_kriegner_cervantes-flores_zhang_moyer_yencho_2002, title={Resistance to sweetpotato chlorotic stunt virus and sweetpotato feathery mottle virus is mediated by two separate recessive genes in sweetpotato}, volume={127}, number={5}, journal={Journal of the American Society for Horticultural Science}, author={Mwanga, R. O. M. and Kriegner, A. and Cervantes-Flores, J. C. and Zhang, D. P. and Moyer, J. W. and Yencho, G. C.}, year={2002}, pages={798–806} } @article{groves_walgenbach_moyer_kennedy_2002, title={The role of weed hosts and tobacco thrips, Frankliniella fusca, in the epidemiology of Tomato spotted wilt virus}, volume={86}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS.2002.86.6.573}, abstractNote={ Wild plant species were systematically sampled to characterize reproduction of thrips, the vector of Tomato spotted wilt virus (TSWV), and natural sources TSWV infection. Thrips populations were monitored on 28 common perennial, biennial, and annual plant species over two noncrop seasons at six field locations across North Carolina. Sonchus asper, Stellaria media, and Taraxacum officianale consistently supported the largest populations of immature TSWV vector species. The tobacco thrips, Frankliniella fusca, was the most abundant TSWV vector species collected, comprising over 95% of vector species in each survey season. Perennial plant species (i.e., Plantago rugelii and Taraxacum officianale) were often only locally abundant, and many annual species (Cerastium vulgatum, Sonchus asper, and Stellaria media) were more widely distributed. Perennial species, including P. rugelii and Rumex crispus, remained TSWV infected for 2 years in a small-plot field test. Where these perennial species are locally abundant, they may serve as important and long-lasting TSWV inoculum sources. In random surveys across 12 locations in North Carolina, TSWV infection was documented by double antibody sandwich enzyme-linked immunosorbent assay in 35 of 72 (49%) common perennial (N = 10), biennial (N = 4), and annual (N = 21) plant species across 18 plant families. Estimated rates of TSWV infection were highest in Cerastium vulgatum (4.2%), Lactuca scariola (1.3%), Molluga verticillata (4.3%), Plantago rugelii (3.4%), Ranunculus sardous (3.6%), Sonchus asper (5.1%), Stellaria media (1.4%), and Taraxacum officianale (5.8%). Nine plant species were determined to be new host recordings for TSWV infection, including Cardamine hirsuta, Eupatorium capillifolium, Geranium carolinianum, Gnaphalium purpureum, Linaria canadense, Molluga verticillata, Pyrrhopappus carolinianus, Raphanus raphanistrum, and Triodanis perfoliata. Our findings document the relative potential of a number of common annual, biennial, and perennial plant species to act as important reproductive sites for F. fusca and as acquisition sources of TSWV for spread to susceptible crops. }, number={6}, journal={PLANT DISEASE}, author={Groves, RL and Walgenbach, JF and Moyer, JW and Kennedy, GG}, year={2002}, month={Jun}, pages={573–582} } @article{hoffmann_qiu_moyer_2001, title={Overcoming host- and pathogen-mediated resistance in tomato and tobacco maps to the M RNA of Tomato spotted wilt virus}, volume={14}, ISSN={["1943-7706"]}, DOI={10.1094/mpmi.2001.14.2.242}, abstractNote={ A viral genetic system was used to map the determinants of the ability of Tomato spotted wilt virus (TSWV) to overcome the R gene (Sw-5) in tomato and the resistance conferred by the nucleocapsid gene of TSWV (N gene) in tobacco. A complete set of reassortant genotypes was generated from TSWV isolates A and D. TSWV-A was able to overcome the Sw-5 gene in tomato and the TSWV N gene in tobacco, whereas TSWV-D was repressed by both forms of resistance. The ability to overcome both forms of resistance was associated with the M RNA segment of TSWV-A (MA). Overcoming the Sw-5 gene was linked solely to the presence of MA, to overcome the TSWV N gene was modified by the L RNA and the S RNA of TSWV-A, which is consistent with previous reports that suggest that the nucleocapsid gene is not the primary determinant for overcoming the nucleocapsid-mediated resistance. Sequence analysis of the M RNA segment of TSWV-A, -D, and the type isolate BR-01 revealed multiple differences in the coding and noncoding regions, which prevented identification of the resistance-breaking nucleotide sequences. }, number={2}, journal={MOLECULAR PLANT-MICROBE INTERACTIONS}, author={Hoffmann, K and Qiu, WP and Moyer, JW}, year={2001}, month={Feb}, pages={242–249} } @article{groves_walgenbach_moyer_kennedy_2001, title={Overwintering of Frankliniella fusca (Thysanoptera : Thripidae) on winter annual weeds infected with Tomato spotted wilt virus and patterns of virus movement between susceptible weed hosts}, volume={91}, ISSN={["1943-7684"]}, DOI={10.1094/PHYTO.2001.91.9.891}, abstractNote={Overwintering of tobacco thrips, Frankliniella fusca, was investigated on common winter annual host plants infected with Tomato spotted wilt virus (TSWV). Populations of tobacco thrips produced on TSWV-infected plants did not differ from those produced on healthy plants, whereas populations varied greatly among host plant species. The mean per plant populations of F. fusca averaged 401, 162, and 10 thrips per plant on Stellaria media, Scleranthus annuus, and Sonchus asper, respectively, during peak abundance in May. Adult F. fusca collected from plant hosts were predominately brachypterous throughout the winter and early spring, but macropterous forms predominated in late spring. Weed hosts varied in their ability to serve as overwintering sources of TSWV inoculum. Following the initial infection by TSWV in October 1997, 75% of Scleranthus annuus and Stellaria media retained infection over the winter and spring season, whereas only 17% of Sonchus asper plants remained infected throughout the same interval. Mortality of TSWV-infected Sonchus asper plants exceeded 25%, but mortality of infected Stellaria media and Scleranthus annuus did not exceed 8%. TSWV transmission by thrips produced on infected plants was greatest on Stellaria media (18%), intermediate on Scleranthus annuus (6%), and lowest on Sonchus asper (2%). Very few viruliferous F. fusca were recovered from soil samples collected below infected wild host plants. Vegetative growth stages of Stellaria media, Sonchus asper, and Ranunculus sardous were more susceptible to F. fusca transmission of TSWV than flowering growth stages, whereas both growth stages of Scleranthus annuus were equally susceptible. In a field study to monitor the spatial and temporal patterns of virus movement from a central source of TSWV-infected Stellaria media to adjacent plots of R. sardous, the incidence of infection in R. sardous plots increased from <1% in March to >42% in June 1999. Infection levels in the Stellaria media inoculum source remained high throughout the experiment, averaging nearly 80% until June 1999 when all Stellaria media plants had senesced. Dispersal of TSWV from the inoculum source extended to the limits of the experimental plot (>37 m). Significant directional patterns of TSWV spread to the R. sardous plots were detected in April and May but not in June. R. sardous infections were detected as early as March and April, suggesting that overwintering inoculum levels in an area can increase rapidly during the spring in susceptible weed hosts prior to planting of susceptible crops. This increase in the abundance of TSWV inoculum sources occurs at a time when vector populations are increasing rapidly. The spread of TSWV among weeds in the spring serves to bridge the period when overwintered inoculum sources decline and susceptible crops are planted.}, number={9}, journal={PHYTOPATHOLOGY}, author={Groves, RL and Walgenbach, JF and Moyer, JW and Kennedy, GG}, year={2001}, month={Sep}, pages={891–899} } @article{magbanua_wilde_roberts_chowdhury_abad_moyer_wetzstein_parrott_2000, title={Field resistance to Tomato spotted wilt virus in transgenic peanut (Arachis hypogaea L.) expressing an antisense nucleocapsid gene sequence}, volume={6}, ISSN={["1572-9788"]}, DOI={10.1023/A:1009649408157}, number={2}, journal={MOLECULAR BREEDING}, author={Magbanua, ZV and Wilde, HD and Roberts, JK and Chowdhury, K and Abad, J and Moyer, JW and Wetzstein, HY and Parrott, WA}, year={2000}, month={Apr}, pages={227–236} } @article{jahn_paran_hoffmann_radwanski_livingstone_grube_aftergoot_lapidot_moyer_2000, title={Genetic mapping of the Tsw locus for resistance to the Tospovirus Tomato spotted wilt virus in Capsicum spp. and its relationship to the Sw-5 gene for resistance to the same pathogen in tomato}, volume={13}, ISSN={["0894-0282"]}, DOI={10.1094/MPMI.2000.13.6.673}, abstractNote={ The Tsw gene conferring dominant resistance to the Tospo-virus Tomato spotted wilt virus (TSWV) in Capsicum spp. has been tagged with a random amplified polymorphic DNA marker and mapped to the distal portion of chromosome 10. No mapped homologues of Sw-5, a phenotypically similar dominant TSWV resistance gene in tomato, map to this region in C. annuum, although a number of Sw-5 homologues are found at corresponding positions in pepper and tomato. The relationship between Tsw and Sw-5 was also examined through genetic studies of TSWV. The capacity of TSWV-A to overcome the Tsw gene in pepper and the Sw-5 gene in tomato maps to different TSWV genome segments. Therefore, despite phenotypic and genetic similarities of resistance in tomato and pepper, we infer that distinct viral gene products control the outcome of infection in plants carrying Sw-5 and Tsw, and that these loci do not appear to share a recent common evolutionary ancestor. }, number={6}, journal={MOLECULAR PLANT-MICROBE INTERACTIONS}, author={Jahn, M and Paran, I and Hoffmann, K and Radwanski, ER and Livingstone, KD and Grube, RC and Aftergoot, E and Lapidot, M and Moyer, J}, year={2000}, month={Jun}, pages={673–682} } @article{qiu_moyer_1999, title={Tomato spotted wilt tospovirus adapts to the TSWV N gene-derived resistance by genome reassortment}, volume={89}, ISSN={["0031-949X"]}, DOI={10.1094/PHYTO.1999.89.7.575}, abstractNote={ Pathogen- and host-derived resistance have been shown to suppress infection by many plant viruses. Tomato spotted wilt tospovirus (TSWV) is among these systems; however, it has easily overcome nearly all host resistance genes and has recently been shown to overcome resistance mediated by the TSWV N gene. To better understand the resistance-breaking mechanisms, we have chosen TSWV N gene-derived resistance (TNDR) as a model to study how plant viruses defeat resistance genes. A defined viral population of isolates TSWV-D and TSWV-10, both suppressed by TNDR, was subjected to TNDR selection by serial passage in an N-gene transgenic plant. The genotype analysis demonstrated that the mixed viral population was driven to form a specific reassortant, L10M10SD, in the presence of TNDR selection, but remained as a heterogeneous mixture in the absence of the selection. A genotype assay of 120 local lesion isolates from the first, fourth, and seventh transfers confirmed the shift of genomic composition. Further analysis demonstrated that the individual L10, M10, and SD RNA segments were each selected independently in response to TNDR selection rather than to a mutation or recombination event. Following the seventh transfer on the N-gene transgenic plants, TSWV S RNA remained essentially identical to the S RNA from TSWV-D, indicating that no intermolecular recombination occurred between the two S RNAs from TSWV-10 and TSWV-D nor with the transferred N gene. These results support the hypothesis that TSWV utilizes genome reassortment to adapt to new host genotypes rapidly and that elements from two or more segments of the genome are involved in suppression of the resistance reaction. }, number={7}, journal={PHYTOPATHOLOGY}, author={Qiu, WP and Moyer, JW}, year={1999}, month={Jul}, pages={575–582} } @article{sherman_moyer_daub_1998, title={A regeneration and Agrobacterium-mediated transformation system for genetically diverse Chrysanthemum cultivars}, volume={123}, ISSN={["0003-1062"]}, DOI={10.21273/jashs.123.2.189}, abstractNote={An efficient, high-frequency regeneration and Agrobacterium-mediated transformation system was developed allowing the genetic engineering of three chrysanthemum (Dendranthema grandiflora Tzvelev) cultivars: the formerly recalcitrant and economically important cut-flower mum `Polaris' and two potted mums, `Hekla' and `Iridon'. The regeneration protocol used leaf explants on a sequence of media with four hormone regimes. Explants were first cultured on an embryogenesis-type medium containing a high concentration of 2,4-D, which promoted callus formation. Shoot primordia were induced by culture on medium lacking 2,4-D, followed by shoot elongation on a high-cytokinin plus gibberellic acid medium. Finally, elongated shoots were rooted on a low-auxin rooting medium. Transformed plants of the three cultivars were obtained following co-culture of leaf explants with A. tumefaciens strain EHA 105 harboring the plasmid pBI121 containing genes for neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS). Stable transformation of the three cultivars was verified via GUS assays and Southern analysis.}, number={2}, journal={JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE}, author={Sherman, JM and Moyer, JW and Daub, ME}, year={1998}, month={Mar}, pages={189–194} } @article{hoffmann_geske_moyer_1998, title={Pathogenesis of tomato spotted wilt virus in peanut plants dually infected with peanut mottle virus}, volume={82}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS.1998.82.6.610}, abstractNote={ Peanut (Arachis hypogaea) plants dually infected with tomato spotted wilt tospovirus (TSWV) and peanut mottle potyvirus (PMV) exhibited a wide variety of symptoms, ranging from PMV-like symptoms of transient mild leaf mottle to TSWV-like symptoms of severe leaf distortion and stunting of the plant. Dual infection did not cause greater symptom severity than infection with either virus alone. In the early stages of disease development, PMV symptoms were similar to the first leaf symptoms of TSWV infection, suggesting that identification of TSWV in field-grown peanuts should not depend on visual observation. The virus titer, determined by enzyme-linked immunosorbent assay, indicated a delay in TSWV disease progress in doubleinfected plants, compared to plants infected with TSWV alone. In the later phase of disease progress, the virus titer in dually infected plants was not significantly different from that of singly infected plants. Infection with TSWV and PMV alone and with both viruses in combination was consistent among commercially grown peanut cultivars. In plants inoculated with TSWV or PMV alone or with both viruses in combination, the length of the latent period and final disease incidence, as measured by the number of plants showing symptoms, did not depend on the cultivar. }, number={6}, journal={PLANT DISEASE}, author={Hoffmann, K and Geske, SM and Moyer, JW}, year={1998}, month={Jun}, pages={610–614} } @article{qiu_geske_hickey_moyer_1998, title={Tomato spotted wilt Tospovirus genome reassortment and genome segment-specific adaptation}, volume={244}, ISSN={["0042-6822"]}, DOI={10.1006/viro.1998.9131}, abstractNote={A system to associate specific genome segments with viral phenotypes and to study factors influencing genome reassortment was developed for tomato spotted wilt Tospovirus (TSWV). Reassortant isolates were generated by co-inoculating a TSWV isolate, TSWV-D, with TSWV-10 or TSWV-MD. The parental origin of each genome segment in putative reassortant isolates was determined by segment-specific restriction fragment length polymorphisms. The TSWV isolates readily exchanged genome segments in a nonrandom fashion. The S RNA from TSWV-D was dominant over the S RNA from TSWV-10. The intergenic region (IGR) of the S RNA was correlated with competitiveness of this genome segment in reassortant isolates. The less competitive S RNA contained a net increase of 62 nt, including a 33-nt duplication in the IGR. This duplicate sequence was highly conserved among isolates from the southeastern United States and an isolate from Bulgaria. The evidence supports the hypothesis that the IGR of the S RNA with an ambisense coding strategy serves a regulatory function which influences the occurrence of this segment in the viral population. In addition, it was demonstrated that stable parental phenotypes can be mapped to specific genome segments as well as generating novel phenotypes not associated with either parent.}, number={1}, journal={VIROLOGY}, author={Qiu, WP and Geske, SM and Hickey, CM and Moyer, JW}, year={1998}, month={Apr}, pages={186–194} } @article{sherman_moyer_daub_1998, title={Tomato spotted wilt virus resistance in chrysanthemum expressing the viral nucleocapsid gene}, volume={82}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS.1998.82.4.407}, abstractNote={ Three tomato spotted wilt virus (TSWV) nucleocapsid (N) gene constructs were employed for Agrobacterium-mediated transformation of chrysanthemum (Dendranthema grandiflora) cv. Polaris. These constructs contained either a full-length N gene (pTSWVN+), a full-length N gene encoding a truncated N protein (pTSWVNt), or an antisense version of the full-length N gene (pTSWVN-), all derived from a dahlia isolate of TSWV (TSWV-D). Initial resistance screens were conducted on cuttings made from 152 pTSWVN+, 37 pTSWVNt, and 47 pTSWVN- transformed plants employing a highly virulent, heterologous strain of TSWV (TSWV-GB) isolated from chrysanthemum and vectored by thrips. This screening served to eliminate the majority of TSWV-susceptible transgenic lines. More rigorous resistance tests with three rounds of mechanical inoculation with TSWV-GB identified one pTSWVNt and two pTSWVN- transformed lines that exhibited a total lack of systemic symptoms and no virus accumulation. Six other lines, including some pTSWVN+, exhibited a lack of one or more of the destructive necrotic TSWV symptoms (stem canker and apical bud death) and a delay in symptom expression. Both sense and antisense constructs, therefore, were found to be effective at yielding TSWV resistance in chrysanthemum. Molecular analysis revealed that the highly TSWV-resistant pTSWVNt line had no detectable levels of N protein. All three resistant lines had low levels of N gene transcript and at least three transgene insertion sites within their genomes, although susceptible lines often had a similar number of insertion sites. The generation of Polaris lines resistant to TSWV transmitted either mechanically or by thrips represents the first time a major ornamental crop has been genetically engineered for disease resistance. }, number={4}, journal={PLANT DISEASE}, author={Sherman, JM and Moyer, JW and Daub, ME}, year={1998}, month={Apr}, pages={407–414} } @inbook{daub_jones_moyer_1997, title={Biotechnological approaches for virus resistance in floral crops}, booktitle={Biotechnology of ornamental plants}, publisher={Wallingford: CAB International}, author={Daub, M. E. and Jones, R. K. and Moyer, J. W.}, editor={R. L. Geneve, J. E. Preece and Merkle, S. A.Editors}, year={1997}, pages={335–351} } @article{daughtrey_jones_moyer_daub_baker_1997, title={Tospoviruses strike the greenhouse industry - INSV has become a major pathogen on flower crops}, volume={81}, ISSN={["0191-2917"]}, DOI={10.1094/PDIS.1997.81.11.1220}, abstractNote={HomePlant DiseaseVol. 81, No. 11Tospoviruses Strike the Greenhouse Industry: INSV Has Become a Major Pathogen on Flower Crops PreviousNext OPENOpen Access licenseTospoviruses Strike the Greenhouse Industry: INSV Has Become a Major Pathogen on Flower CropsMargery L. Daughtrey, Ronald K. Jones, James W. Moyer, Margaret E. Daub, and James R. BakerMargery L. DaughtreySearch for more papers by this author, Ronald K. JonesSearch for more papers by this author, James W. MoyerSearch for more papers by this author, Margaret E. DaubSearch for more papers by this author, and James R. BakerSearch for more papers by this authorAffiliationsAuthors and Affiliations Margery L. Daughtrey , L. I. Horticultural Research Laboratory, Cornell University, Riverhead, NY Ronald K. Jones James W. Moyer Margaret E. Daub , Department of Plant Pathology, North Carolina State University, Raleigh James R. Baker , Department of Entomology, North Carolina State University, Raleigh Published Online:22 Feb 2007https://doi.org/10.1094/PDIS.1997.81.11.1220AboutSectionsPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat DetailsFiguresLiterature CitedRelated Vol. 81, No. 11 November 1997SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 25 Jan 2008Published: 22 Feb 2007 Pages: 1220-1230 Information© 1997 The American Phytopathological SocietyPDF downloadCited byMolecular characterization and incidence of new tospovirus: Soybean Vein Necrosis Virus (SVNV) in Egypt1 January 2024 | Brazilian Journal of Biology, Vol. 84Soğuk Sıcaklıkta Fosfin Fümigasyonunun Karanfil üzerindeki Frankliniella occidentalis Perg. 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Goldbach22 February 2007 | Phytopathology®, Vol. 93, No. 1Agrobacterium - mediated Transformation of Chrysanthemum (Dendranthema grandiflora) Plants with a Disease Resistance Gene (pac1)Plant Biotechnology, Vol. 20, No. 2RT-PCR for detecting five distinct Tospovirus species using degenerate primers and dsRNA templateJournal of Virological Methods, Vol. 96, No. 2An Anatomical Perspective of Tospovirus TransmissionVariation in tospovirus transmission between populations of Frankliniella occidentalis (Thysanoptera: Thripidae)9 March 2007 | Bulletin of Entomological Research, Vol. 89, No. 6Assessing the susceptibility of chrysanthemum cultivars to tomato spotted wilt virus4 January 2002 | Plant Pathology, Vol. 48, No. 6EPPO DATA SHEETS ON QUARANTINE PESTS.EPPO Bulletin, Vol. 29, No. 4}, number={11}, journal={PLANT DISEASE}, author={Daughtrey, ML and Jones, RK and Moyer, JW and Daub, ME and Baker, JR}, year={1997}, month={Nov}, pages={1220–1230} } @article{moyer_j. w._1996, title={Molecular and genetic determinants of diversity in tomato spotted wilt virus}, ISBN={9066058285}, DOI={10.17660/actahortic.1996.431.19}, number={431}, journal={Acta Horticulturae}, author={Moyer and J. W., Qiu WenPing}, year={1996}, pages={219} }