@article{rangel_böröczky_schal_tarpy_2016, title={Honey Bee (Apis mellifera) Queen Reproductive Potential Affects Queen Mandibular Gland Pheromone Composition and Worker Retinue Response}, volume={11}, ISSN={1932-6203}, url={http://dx.doi.org/10.1371/journal.pone.0156027}, DOI={10.1371/journal.pone.0156027}, abstractNote={Reproductive division of labor is one of the defining traits of honey bees (Apis mellifera), with non-reproductive tasks being performed by workers while a single queen normally monopolizes reproduction. The decentralized organization of a honey bee colony is maintained in large part by a bouquet of queen-produced pheromones, the distribution of which is facilitated by contact among workers throughout the hive. Previous studies have shown that the developmental fate of honey bee queens is highly plastic, with queens raised from younger worker larvae exhibiting higher measures of reproductive potential compared to queens raised from older worker larvae. We investigated differences in the chemical composition of the mandibular glands and attractiveness to workers of “high-quality” queens (i.e., raised from first instar worker larvae; more queen-like) and “low-quality” queens (i.e., raised from third instar worker larvae; more worker-like). We characterized the chemical profiles of the mandibular glands of high-quality queens and low-quality queens using GC-MS and used the worker retinue response as a measure of the attractiveness to workers of high-quality queens vs. low-quality queens. We found that queen quality affected the chemical profiles of mandibular gland contents differently across years, showing significant differences in the production of the queen mandibular pheromone (“QMP”) components HVA and 9-HDA in 2010, but no significant differences of any glandular compound in 2012. We also found that workers were significantly more attracted to high-quality queens than to low-quality queens in 2012, possibly because of increased attractiveness of their mandibular gland chemical profiles. Our results indicate that the age at which honey bee larvae enter the “queen-specific” developmental pathway influences the chemical composition of queen mandibular glands and worker behavior. However, these changes are not consistent across years, suggesting that other external factors may play important roles in modulating queen quality.}, number={6}, journal={PLOS ONE}, publisher={Public Library of Science (PLoS)}, author={Rangel, Juliana and Böröczky, Katalin and Schal, Coby and Tarpy, David R.}, editor={Nascimento, Fabio S.Editor}, year={2016}, month={Jun}, pages={e0156027} }
 @article{boroczky_zylstra_mccartney_mastro_tumlinson_2012, title={Volatile Profile Differences and the Associated Sirex noctilio Activity in Two Host Tree Species in the Northeastern United States}, volume={38}, number={2}, journal={Journal of Chemical Ecology}, author={Boroczky, K. and Zylstra, K. E. and McCartney, N. B. and Mastro, V. C. and Tumlinson, J. H.}, year={2012}, pages={213–221} }
 @article{ponnusamy_böröczky_wesson_schal_apperson_2011, title={Bacteria Stimulate Hatching of Yellow Fever Mosquito Eggs}, volume={6}, ISSN={1932-6203}, url={http://dx.doi.org/10.1371/journal.pone.0024409}, DOI={10.1371/journal.pone.0024409}, abstractNote={Background Aedes aegypti Linnaeus is a peridomestic mosquito that lays desiccation-resistant eggs in water-filled human-made containers. Previous investigations connected egg hatching with declining dissolved oxygen (DO) that is associated with bacterial growth. However, past studies failed to uncouple DO from other potential stimulatory factors and they contained little quantitative information about the microbial community; consequently, a direct role for bacteria or compounds associated with bacteria in stimulating egg hatching cannot be dismissed. Methodology/Principal Findings Environmental factors stimulating hatch of Ae. aegypti eggs were investigated using non-sterile and sterile white oak leaf (WOL) infusions and a bacterial culture composed of a mix of 14 species originally isolated from bamboo leaf infusion. In WOL infusion with active microbes, 92.4% of eggs hatched in 2-h at an average DO concentration of 2.4 ppm. A 24-h old bacterial culture with a DO concentration of 0.73 ppm also stimulated 95.2% of eggs hatch within 1-h. In contrast, only 4.0% of eggs hatched in sterile infusion, whose DO averaged 7.4 ppm. Effects of bacteria were uncoupled from DO by exposing eggs to bacterial cells suspended in NaCl solution. Over a 4-h exposure period, 93.8% of eggs hatched while DO concentration changed minimally from 7.62 to 7.50 ppm. Removal of bacteria by ultra-filtration and cell-free filtrate resulted in only 52.0% of eggs hatching after 4-h at an average DO concentration of 5.5 ppm. Conclusions/Significance Collectively, the results provide compelling evidence that bacteria or water-soluble compounds secreted by bacteria, not just low DO concentration, stimulate hatching of Ae. aegypti eggs. However, the specific cues involved remain to be identified. These research findings contribute new insight into an important aspect of the oviposition biology of Ae. aegypti, a virus vector of global importance, providing the basis for a new paradigm of environmental factors involved in egg hatching.}, number={9}, journal={PLoS ONE}, publisher={Public Library of Science (PLoS)}, author={Ponnusamy, Loganathan and Böröczky, Katalin and Wesson, Dawn M. and Schal, Coby and Apperson, Charles S.}, editor={Leulier, FrançoisEditor}, year={2011}, month={Sep}, pages={e24409} }
 @article{ponnusamy_xu_böröczky_wesson_abu ayyash_schal_apperson_2010, title={Oviposition Responses of the Mosquitoes Aedes aegypti and Aedes albopictus to Experimental Plant Infusions in Laboratory Bioassays}, volume={36}, ISSN={0098-0331 1573-1561}, url={http://dx.doi.org/10.1007/s10886-010-9806-2}, DOI={10.1007/s10886-010-9806-2}, abstractNote={Attraction of the mosquitoes Aedes aegypti and Ae. albopictus to plant infusions was evaluated by using a modified sticky-screen bioassay that improved the resolution of mosquito responses to odorants. Under bioassay conditions, solid-phase microextraction-gas chromatographic analyses of the volatile marker chemical indole showed that odorants diffused from bioassay cups, forming a concentration gradient. Infusions were prepared by separately fermenting senescent leaves of eight plant species in well water. Plant infusions were evaluated over an 8-fold range of leaf biomass and/or a 28 d fermentation period. The responses of gravid females of both mosquito species varied with the plant species and biomass of plant materials used to make infusions, and with the length of the fermentation period. Infusions made from senescent bamboo (Arundinaria gigantea) and white oak (Quercus alba) leaves were significantly attractive to both mosquitoes. In general, infusions prepared by using low biomass of plant material over a 7–14 d fermentation period were most attractive to Ae. aegypti. In contrast, Ae. albopictus was attracted to infusions made using a wider range of plant biomass and over a longer fermentation period. Both mosquito species were more attracted to a non-sterile white oak leaf infusion than to white oak leaf infusion that was prepared using sterilized plant material and water, thus suggesting a role for microbial activity in the production of odorants that mediate the oviposition response of gravid mosquitoes.}, number={7}, journal={Journal of Chemical Ecology}, publisher={Springer Science and Business Media LLC}, author={Ponnusamy, Loganathan and Xu, Ning and Böröczky, Katalin and Wesson, Dawn M. and Abu Ayyash, Luma and Schal, Coby and Apperson, Charles S.}, year={2010}, month={Jun}, pages={709–719} }
 @article{boeroeczky_crook_jones_kenny_zylstra_mastro_tumlinson_2009, title={Monoalkenes as Contact Sex Pheromone Components of the Woodwasp Sirex noctilio}, volume={35}, ISSN={["1573-1561"]}, DOI={10.1007/s10886-009-9693-6}, abstractNote={A pheromone on the cuticle of females of the woodwasp Sirex noctilio, a recently introduced pest of pines in North America, induces conspecific males to attempt copulation. Dead females washed with hexane did not elicit copulation attempts from males, whereas reapplication of a female hexane body wash onto the cuticle of dead females elicited copulation attempts by 65% of males tested. Analysis of the hexane extract revealed saturated and unsaturated hydrocarbons as major components of the female cuticle. Behavior-guided fractionation of the female body wash led to the identification of three components, (Z)-7-heptacosene, (Z)-7-nonacosene, and (Z)-9-nonacosene, of the sex pheromone of S. noctilio that elicited copulatory responses from males.}, number={10}, journal={JOURNAL OF CHEMICAL ECOLOGY}, author={Boeroeczky, Katalin and Crook, Damon J. and Jones, Tappey H. and Kenny, Joshua C. and Zylstra, Kelley E. and Mastro, Victor C. and Tumlinson, James H.}, year={2009}, month={Oct}, pages={1202–1211} }