@misc{maren_duan_da_yencho_ranney_liu_2022, title={Genotype-independent plant transformation}, volume={9}, ISSN={["2052-7276"]}, DOI={10.1093/hr/uhac047}, abstractNote={Abstract}, journal={HORTICULTURE RESEARCH}, author={Maren, Nathan A. and Duan, Hui and Da, Kedong and Yencho, G. Craig and Ranney, Thomas G. and Liu, Wusheng}, year={2022}, month={Jan} }
 @article{harmon_touchell_ranney_da_liu_2022, title={Tissue Culture and Regeneration of Three Rose Cultivars}, volume={57}, ISSN={["2327-9834"]}, DOI={10.21273/HORTSCI16716-22}, abstractNote={Methods of in vitro regeneration protocols were developed for three elite rose cultivars, Chewnicebell (Oso Easy Italian Ice®), Bucbi (Carefree Beauty™), and Cheweyesup (Ringo All-Star™). We evaluated the effects of different types and concentrations of auxins [dichlorophenoxyacetic acid (2,4-D) and trichlorophenoxyacetic acid (2,4,5-T)], carbohydrates [sucrose, glucose, and fructose], and cytokinins [thidiazuron (TDZ) and 6-bezylaminopurine (BAP)] on callus induction and regeneration from leaf explants. The greatest amount of regenerative callus was obtained on media containing 10 µM 2,4-D and 30 g·L−1 sucrose for Italian Ice® (40%), 10 µM 2,4-D and 60 g·L−1 glucose for Carefree Beauty™ (24%), and 5 µM 2,4,5-T and 30 g·L−1 sucrose for Ringo All-Star™ (32%). The greatest regeneration occurred when callus was transferred to media consisting of 1/2 MS media supplemented with 2.9 µM GA3 and 5 µM TDZ for Italian Ice® and Ringo All-Star™, and with 2.9 µM GA3 and 20 µM TDZ for Carefree Beauty™. Plantlets regenerated from callus were cultured on maintenance media and successfully transferred ex vitro. This study highlights the genotype-specific responses among rose cultivars and provides the first reports of in vitro regeneration for Italian Ice® and Ringo All-Star™.}, number={11}, journal={HORTSCIENCE}, author={Harmon, Davis D. and Touchell, Darren H. and Ranney, Thomas G. and Da, Kedong and Liu, Wusheng}, year={2022}, month={Nov}, pages={1430–1435} }