@misc{maren_duan_da_yencho_ranney_liu_2022, title={Genotype-independent plant transformation}, volume={9}, ISSN={["2052-7276"]}, DOI={10.1093/hr/uhac047}, abstractNote={Abstract Plant transformation and regeneration remain highly species- and genotype-dependent. Conventional hormone-based plant regeneration via somatic embryogenesis or organogenesis is tedious, time-consuming, and requires specialized skills and experience. Over the last 40 years, significant advances have been made to elucidate the molecular mechanisms underlying embryogenesis and organogenesis. These pioneering studies have led to a better understanding of the key steps and factors involved in plant regeneration, resulting in the identification of crucial growth and developmental regulatory genes that can dramatically improve regeneration efficiency, shorten transformation time, and make transformation of recalcitrant genotypes possible. Co-opting these regulatory genes offers great potential to develop innovative genotype-independent genetic transformation methods for various plant species, including specialty crops. Further developing these approaches has the potential to result in plant transformation without the use of hormones, antibiotics, selectable marker genes, or tissue culture. As an enabling technology, the use of these regulatory genes has great potential to enable the application of advanced breeding technologies such as genetic engineering and gene editing for crop improvement in transformation-recalcitrant crops and cultivars. This review will discuss the recent advances in the use of regulatory genes in plant transformation and regeneration, and their potential to facilitate genotype-independent plant transformation and regeneration.}, journal={HORTICULTURE RESEARCH}, author={Maren, Nathan A. and Duan, Hui and Da, Kedong and Yencho, G. Craig and Ranney, Thomas G. and Liu, Wusheng}, year={2022}, month={Jan} }
 @article{harmon_touchell_ranney_da_liu_2022, title={Tissue Culture and Regeneration of Three Rose Cultivars}, volume={57}, ISSN={["2327-9834"]}, DOI={10.21273/HORTSCI16716-22}, abstractNote={Methods of in vitro regeneration protocols were developed for three elite rose cultivars, Chewnicebell (Oso Easy Italian Ice ® ), Bucbi (Carefree Beauty™), and Cheweyesup (Ringo All-Star™). We evaluated the effects of different types and concentrations of auxins [dichlorophenoxyacetic acid (2,4-D) and trichlorophenoxyacetic acid (2,4,5-T)], carbohydrates [sucrose, glucose, and fructose], and cytokinins [thidiazuron (TDZ) and 6-bezylaminopurine (BAP)] on callus induction and regeneration from leaf explants. The greatest amount of regenerative callus was obtained on media containing 10 µM 2,4-D and 30 g·L −1 sucrose for Italian Ice ® (40%), 10 µM 2,4-D and 60 g·L −1 glucose for Carefree Beauty™ (24%), and 5 µM 2,4,5-T and 30 g·L −1 sucrose for Ringo All-Star™ (32%). The greatest regeneration occurred when callus was transferred to media consisting of 1/2 MS media supplemented with 2.9 µM GA 3 and 5 µM TDZ for Italian Ice ® and Ringo All-Star™, and with 2.9 µM GA 3 and 20 µM TDZ for Carefree Beauty™. Plantlets regenerated from callus were cultured on maintenance media and successfully transferred ex vitro. This study highlights the genotype-specific responses among rose cultivars and provides the first reports of in vitro regeneration for Italian Ice ® and Ringo All-Star™.}, number={11}, journal={HORTSCIENCE}, author={Harmon, Davis D. and Touchell, Darren H. and Ranney, Thomas G. and Da, Kedong and Liu, Wusheng}, year={2022}, month={Nov}, pages={1430–1435} }