Kevin Garcia

https://orcid.org/0000-0003-0821-1024

Symbiosis, Mycorrhizae, Transport, Nutrient Exchanges, Fungal Genetics

My laboratory focuses on deciphering the molecular basis of bi-directional nutrient fluxes between plant roots and soil microbes, with a particular emphasis on arbuscular mycorrhizal and ectomycorrhizal associations. Mycorrhizal fungi considerably improve nutrition of the host plant. Therefore, it is conceivable that utilizing these natural symbioses will reduce the amount of fertilizers in future agricultural and forestry practices. We explore the molecular players participating in nutrient allocation in arbuscular mycorrhizal and ectomycorrhizal associations using physiological, biochemical, molecular, genetic, and transcriptomic approaches. Our research contributes to the harnessing of plant-microbe associations to improve nutrient use efficiency and tolerance to global environmental changes in agroecosystems.

Works (41)

2023 journal article

Development of split-root assays for loblolly pine (Pinus taeda L.) seedlings to study ectomycorrhizal symbioses

MethodsX.

By: B. Rose, H. Frank & K. Garcia

Source: ORCID
Added: February 6, 2023

2023 journal article

The putative transporter MtUMAMIT14 participates in nodule formation in Medicago truncatula

Scientific Reports, 13(1).

By: K. Garcia, K. Cloghessy*, D. Cooney n, B. Shelley*, S. Chakraborty*, A. Kafle n, A. Busidan*, U. Sonawala* ...

Source: ORCID
Added: January 21, 2023

2022 journal article

Cesium could be used as a proxy for potassium in mycorrhizal Medicago truncatula

PLANT SIGNALING & BEHAVIOR, 17(1).

By: A. Kafle & K. Garcia

author keywords: Arbuscular mycorrhizal symbiosis; cesium; Medicago truncatula; potassium; Rhizophagus irregularis
Sources: ORCID, Web Of Science
Added: October 20, 2022

2022 journal article

Mycorrhiza-mediated potassium transport in Medicago truncatula can be evaluated by using rubidium as a proxy

PLANT SCIENCE, 322.

By: A. Kafle, D. Cooney, G. Shah n & K. Garcia

author keywords: Arbuscular mycorrhizal symbiosis; Medicago truncatula; Plant nutrition; Potassium; Rhizophagus irregularis; Rubidium
Sources: Web Of Science, ORCID
Added: August 8, 2022

2022 journal article

NPK fertilizer use in loblolly pine plantations: Who are we really feeding?

FOREST ECOLOGY AND MANAGEMENT, 520.

By: J. Hackman, B. Rose, H. Frank, R. Vilgalys, R. Cook & K. Garcia

author keywords: Ectomycorrhizal symbiosis; Loblolly pine; Nutrient use efficiency; Plant nutrition
Sources: ORCID, Web Of Science
Added: July 6, 2022

2022 article

Physiological and transcriptomic response of Medicago truncatula to colonization by high- or low-benefit arbuscular mycorrhizal fungi

Cope, K. R., Kafle, A., Yakha, J. K., Pfeffer, P. E., Strahan, G. D., Garcia, K., … Bucking, H. (2022, May 5). MYCORRHIZA, Vol. 32.

author keywords: Arbuscular mycorrhizal fungi; Gibberellic acid; Nutrient exchange; Nitrogen; Phosphorus; Strigolactone
Sources: ORCID, Web Of Science
Added: May 6, 2022

2022 journal article

Readily available resources across sites and genotypes result in greater aboveground growth and reduced fine root production in Pinus taeda

FOREST ECOLOGY AND MANAGEMENT, 521.

By: T. Shively n, R. Cook, C. Maier, K. Garcia, T. Albaugh, O. Campoe, Z. Leggett

Sources: ORCID, Web Of Science
Added: September 23, 2022

2022 journal article

Secrets of the fungus-specific potassium channel TOK family

Trends in Microbiology, 12.

By: G. Houdinet n, C. Guerrero-Galán*, B. Rose n, K. Garcia & S. Zimmermann*

Source: ORCID
Added: January 21, 2023

2022 review

Split down the middle: studying arbuscular mycorrhizal and ectomycorrhizal symbioses using split-root assays

(M. Gifford, Ed.) [Review of ]. JOURNAL OF EXPERIMENTAL BOTANY, 73(5), 1288–1300.

By: A. Kafle, H. Frank n, B. Rose n & K. Garcia

Ed(s): M. Gifford

author keywords: Arbuscular mycorrhizal symbiosis; carbon; ectomycorrhizal symbiosis; legumes; nitrogen; nutrient transport; phosphorus; split-root; trees
Sources: ORCID, Web Of Science
Added: November 18, 2021

2021 article

Benefits provided by four ectomycorrhizal fungi to Pinus taeda under different external potassium availabilities

Frank, H. E. R., & Garcia, K. (2021, August 25). MYCORRHIZA, Vol. 8.

By: H. Frank n & K. Garcia

author keywords: Ectomycorrhizal symbiosis; Pinus taeda; Plant nutrition; Potassium; Rubidium; Sodium
Sources: ORCID, Web Of Science
Added: August 26, 2021

2021 review

Mycorrhizal Symbiosis for Better Adaptation of Trees to Abiotic Stress Caused by Climate Change in Temperate and Boreal Forests

[Review of ]. FRONTIERS IN FORESTS AND GLOBAL CHANGE, 4.

By: M. Usman*, T. Ho-Plagaro*, H. Frank n, M. Calvo-Polanco*, I. Gaillard*, K. Garcia, S. Zimmermann*

author keywords: mycorrhizal symbioses; trees; climate change; environmental abiotic stress; high temperature; drought; salinity; flooding
Sources: ORCID, Web Of Science
Added: October 5, 2021

2020 journal article

Editorial: Importance of Root Symbiomes for Plant Nutrition: New Insights, Perspectives and Future Challenges

Frontiers in Plant Science, 11.

By: K. Garcia, H. Bücking* & S. Zimmermann*

author keywords: biological nitrogen fixation; mycorrhizal symbiosis; plant growth promoting rhizobacteria; plant nutrition; root microbiome
Source: ORCID
Added: June 3, 2020

2020 journal article

Fungal Shaker-like channels beyond cellular K+ homeostasis: A role in ectomycorrhizal symbiosis between Hebeloma cylindrosporum and Pinus pinaster

PLOS ONE.

By: K. Garcia, C. Guerrero-Galán*, H. Frank n, M. Haider*, A. Delteil*, G. Conéjéro*, R. Lambilliotte*, C. Fizames*, H. Sentenac*, S. Zimmermann*

Ed(s): E. Kothe

Source: ORCID
Added: November 21, 2020

2020 journal article

Lipo-chitooligosaccharides as regulatory signals of fungal growth and development

NATURE COMMUNICATIONS, 11(1).

By: T. Rush*, V. Puech-Pages*, A. Bascaules*, P. Jargeat*, F. Maillet*, A. Haouy*, A. Maes*, C. Carriel* ...

Sources: Web Of Science, ORCID
Added: September 7, 2020

2020 journal article

Micronutrient transport in mycorrhizal symbiosis; zinc steals the show

Fungal Biology Reviews.

By: J. Ruytinx*, A. Kafle n, M. Usman*, L. Coninx*, S. Zimmermann* & K. Garcia

author keywords: Arbuscular mycorrhizal symbiosis; Cation diffusion facilitator; Ectomycorrhizal symbiosis; Fungal homeostasis; Nutrient transport; Zinc-iron permease
Source: ORCID
Added: October 1, 2019

2020 article

Physiological and transcriptomic response of Medicago truncatula to colonization with high and low benefit arbuscular mycorrhizal fungi

Cope, K. R., Kafle, A., Yakha, J. K., Pfeffer, P. E., Strahan, G. D., Garcia, K., … Bücking, H. (2020, December 12). (Vol. 12). Vol. 12.

By: K. Cope*, A. Kafle n, J. Yakha*, P. Pfeffer*, G. Strahan*, K. Garcia, S. Subramanian*, H. Bücking*

Source: ORCID
Added: December 13, 2020

2020 journal article

Role of cytosolic, tyrosine‐insensitive prephenate dehydrogenase in Medicago truncatula

Plant Direct, 4(5).

By: C. Schenck*, J. Westphal*, D. Jayaraman*, K. Garcia, J. Wen*, K. Mysore*, J. Ané*, L. Sumner*, H. Maeda*

author keywords: legumes; prephenate dehydrogenase; the shikimate pathway; TyrA dehydrogenase; tyrosine
Source: ORCID
Added: June 3, 2020

2019 article

Beneficial Plant Microbe Interactions and Their Effect on Nutrient Uptake, Yield, and Stress Resistance of Soybeans

Kafle, A., Garcia, K., Peta, V., Yakha, J., Soupir, A., & Bücking, H. (2019, February). Soybean - Biomass, Yield and Productivity.

By: A. Kafle, K. Garcia, V. Peta, J. Yakha, A. Soupir & H. Bücking

Source: ORCID
Added: March 6, 2019

2019 journal article

Harnessing Soil Microbes to Improve Plant Phosphate Efficiency in Cropping Systems

Agronomy, 9(3), 127.

By: A. Kafle, K. Cope, R. Raths, J. Yakha, S. Subramanian, H. Bücking, K. Garcia

author keywords: arbuscular mycorrhizal symbiosis; ectomycorrhizal symbiosis; phosphate solubilizing bacteria; phosphate efficiency
Source: ORCID
Added: March 9, 2019

2019 journal article

Nutrient demand and fungal access to resources control the carbon allocation to the symbiotic partners in tripartite interactions of Medicago truncatula

Plant, Cell & Environment, 42(1), 270–284.

By: A. Kafle*, K. Garcia, X. Wang*, P. Pfeffer*, G. Strahan* & H. Bücking*

author keywords: arbuscular mycorrhizal symbiosis; carbon transport; Ensifer meliloti; legumes; nitrogen uptake; rhizobia; Rhizophagus irregularis; sucrose transport; sucrose uptake transporter (SUT); sugars will eventually be exported transporter (SWEET)
Source: ORCID
Added: February 4, 2019

2019 journal article

Phosphorus Transport in Mycorrhiza: How Far Are We?

Trends in Plant Science, 24(9), 794–801.

By: C. Plassard*, A. Becquer* & K. Garcia

Source: ORCID
Added: July 2, 2019

2019 journal article

The Ectomycorrhizal Fungus Laccaria bicolor Produces Lipochitooligosaccharides and Uses the Common Symbiosis Pathway to Colonize Populus Roots

The Plant Cell, 31(10), 2386–2410.

Source: ORCID
Added: October 9, 2019

2018 journal article

HcPT1.2 participates in Pi acquisition in Hebeloma cylindrosporum external hyphae of ectornycorrhizas under high and low phosphate conditions

PLANT SIGNALING & BEHAVIOR, 13(10).

By: A. Becquer*, K. Garcia & C. Plassard*

author keywords: Ectomycorrhizal symbiosis; H+:Pi transporter; Immunolocalization; Inorganic phosphate; RT-qPCR; Yeast complementation
Sources: Web Of Science, ORCID
Added: November 26, 2018

2018 journal article

HcTOK1 participates in the maintenance of K+ homeostasis in the ectomycorrhizal fungus Hebeloma cylindrosporum, which is essential for the symbiotic K+ nutrition of Pinus pinaster

Plant Signaling & Behavior, 13(6), e1480845.

By: C. Guerrero-Galán*, K. Garcia, G. Houdinet* & S. Zimmermann*

author keywords: ectomycorrhizal symbiosis; ion homeostasis; plant nutrition; potassium transport; subcellular localization; TOK channel
Source: ORCID
Added: February 4, 2019

2018 journal article

Plant potassium nutrition in ectomycorrhizal symbiosis: properties and roles of the three fungal TOK potassium channels in Hebeloma cylindrosporum.

Environmental Microbiology, 4(5), 1873–1887.

By: C. Guerrero-Galán*, A. Delteil*, K. Garcia, G. Houdinet*, G. Conéjéro*, I. Gaillard*, H. Sentenac*, S. Zimmermann*

Source: ORCID
Added: February 4, 2019

2018 journal article

The Hebeloma cylindrosporum HcPT2 Pi transporter plays a key role in ectomycorrhizal symbiosis

New Phytologist, 6.

By: A. Becquer*, K. Garcia, L. Amenc*, C. Rivard*, J. Doré*, C. Trives-Segura*, W. Szponarski*, S. Russet* ...

author keywords: ectomycorrhizal (ECM) fungus; overexpression; P-32 labeling; phosphate transporter; Pinus pinaster; RNA interference; X-ray fluorescence mapping
Source: ORCID
Added: February 4, 2019

2018 article

The Role of Plant Transporters in Mycorrhizal Symbioses

MEMBRANE TRANSPORT IN PLANTS, Vol. 87, pp. 303–342.

By: C. Guerrero-Galan*, G. Houdinet*, M. Calvo-Polanco*, K. Bonaldi*, K. Garcia & S. Zimmermann*

Sources: Web Of Science, ORCID
Added: January 7, 2019

2018 article

The ectomycorrhizal contribution to tree nutrition

Advances in Botanical Research.

By: A. Becquer*, C. Guerrero-Galán*, J. Eibensteiner*, G. Houdinet*, H. Bücking*, S. Zimmermann*, K. Garcia

Source: ORCID
Added: February 4, 2019

2017 journal article

Physiological responses and gene co-expression network of mycorrhizal roots under K+ deprivation

Plant Physiology, 2(3), pp.01959.2016.

By: K. Garcia, D. Chasman*, S. Roy* & J. Ane*

Source: ORCID
Added: February 4, 2019

2017 journal article

Polymorphic responses of Medicago truncatula accessions to potassium deprivation.

Plant Signaling & Behavior, 12(4), e1307494.

By: K. Garcia & J. Ané*

author keywords: Development; legumes; Medicago truncatula; potassium; reactive oxygen species
Source: ORCID
Added: February 4, 2019

2016 journal article

A proteomic atlas of the legume Medicago truncatula and its nitrogen-fixing endosymbiont Sinorhizobium meliloti

Nature Biotechnology, 34(11), 1198–1205.

By: H. Marx*, C. Minogue*, D. Jayaraman*, A. Richards*, N. Kwiecien*, A. Siahpirani*, S. Rajasekar*, J. Maeda* ...

Source: ORCID
Added: February 4, 2019

2016 journal article

Comparative Analysis of Secretomes from Ectomycorrhizal Fungi with an Emphasis on Small-Secreted Proteins

Frontiers in Microbiology, 7.

By: K. Garcia & J. Ané*

author keywords: ectomycorrhizal; saprotrophs; secretomics; small-secretedproteins; symbiosis; secretomes
Source: ORCID
Added: February 4, 2019

2016 journal article

Symbiotic Nitrogen Fixation and the Challenges to Its Extension to Nonlegumes

Applied and Environmental Microbiology, 82(13), 3698–3710.

Ed(s): R. Kelly

Source: ORCID
Added: February 4, 2019

2016 journal article

Take a Trip Through the Plant and Fungal Transportome of Mycorrhiza

Trends in Plant Science, 21(11), 937–950.

By: K. Garcia, J. Doidy*, S. Zimmermann*, D. Wipf* & P. Courty*

Source: ORCID
Added: February 4, 2019

2016 article

The transportome of mycorrhizal systems

Courty, P.-E., Doidy, J., Garcia, K., Wipf, D., & Zimmermann, S. D. (2016, October). Molecular Mycorrhizal Symbiosis, pp. 239–256.

By: P. Courty*, J. Doidy*, K. Garcia, D. Wipf* & S. Zimmermann*

Source: ORCID
Added: February 4, 2019

2015 journal article

Molecular signals required for the establishment and maintenance of ectomycorrhizal symbioses

New Phytologist, 208(1), 79–87.

By: K. Garcia, P. Delaux*, K. Cope* & J. Ané*

author keywords: arbuscular mycorrhiza; common symbiosis pathway; ectomycorrhiza; effectors; nitrogen (N); nutrient exchange; phosphorus (P); signaling
Source: ORCID
Added: February 4, 2019

2014 journal article

Potassium nutrition of ectomycorrhizal Pinus pinaster : overexpression of the Hebeloma cylindrosporum Hc Trk1 transporter affects the translocation of both K + and phosphorus in the host plant

New Phytologist, 201(3), 951–960.

By: K. Garcia, A. Delteil*, G. Conéjéro*, A. Becquer*, C. Plassard*, H. Sentenac*, S. Zimmermann*

author keywords: ectomycorrhizal symbiosis; Hebeloma cylindrosporum; in situ hybridization; overexpression; phosphate transporter HcPT1.1; plant potassium nutrition; potassium-sodium transporter HcTrk1; protein-EGFP fusion
Source: ORCID
Added: February 4, 2019

2014 journal article

The role of mycorrhizal associations in plant potassium nutrition

Frontiers in Plant Science, 5.

By: K. Garcia & S. Zimmermann*

author keywords: potassium; plant nutrition; arbuscular mycorrhizal symbiosis; ectomycorrhizal symbiosis; transport systems
Source: ORCID
Added: February 4, 2019

2013 journal article

Biotrophic transportome in mutualistic plant–fungal interactions

Mycorrhiza, 23(8), 597–625.

By: L. Casieri*, N. Lahmidi*, J. Doidy*, C. Veneault-Fourrey*, A. Migeon*, L. Bonneau*, P. Courty*, K. Garcia ...

author keywords: Mycorrhiza; Nutrient transport; Transporters
Source: ORCID
Added: February 4, 2019

2013 journal article

Mycorrhiza for all: an under-earth revolution

New Phytologist, 198(3), 652–655.

author keywords: agriculture; biogeography; community ecology; evolution; genomics; inoculum; mycorrhizal fungi; physiology
Source: ORCID
Added: February 4, 2019

2013 journal article

Promoter-dependent expression of the fungal transporter HcPT1.1 under Pi shortage and its spatial localization in ectomycorrhiza

Fungal Genetics and Biology, 58-59, 53–61.

By: K. Garcia, M. Haider*, A. Delteil*, C. Corratgé-Faillie*, G. Conéjero*, M. Tatry*, A. Becquer*, L. Amenc* ...

author keywords: Ectomycorrhizal (ECM) symbiosis; Hebeloma cylindrosporum phosphate transporter HcPT1.1; Agrotransformation; GFP-promoter expression; In situ hybridization; Protein immunolocalization
Source: ORCID
Added: February 4, 2019

Employment

2018 - present

North Carolina State University Raleigh, NC, US
Assistant Professor Crop and Soil Sciences

2017 - 2018

South Dakota State University Brookings, SD, US
Postdoctoral Researcher Biology and Microbiology

2014 - 2017

University of Wisconsin Madison Madison, WI, US
Postdoctoral Researcher Bacteriology

Education

2010 - 2013

Université de Montpellier Montpellier, Languedoc-Roussillon, FR
Ph.D. in Integrative Plant Biology B&PMP

2008 - 2010

Université de Montpellier Montpellier, Languedoc-Roussillon, FR
M.S. in Functional Biology of Plants

2005 - 2008

Université Jean Monnet Saint-Etienne Saint-Etienne, Rhône-Alpes, FR
B.S. in Biology of Organisms

Funding History

Funding history based on the linked ORCID record.

grant September 15, 2020 - September 14, 2023
Impact of the Arbuscular Mycorrhizal Symbiosis on the Physiological and Molecular Responses of Legumes to Potassium Deprivation
National Institute of Food and Agriculture
grant October 1, 2018 - September 30, 2023
Impact of the Arbuscular Mycorrhizal Symbiosis on the Physiological and Molecular Responses of Legumes and Cereals to Potassium Deprivation
National Institute of Food and Agriculture