@article{alviar_rotenberg_martin_whitfield_2022, title={The physical interactome between Peregrinus maidis proteins and the maize mosaic virus glycoprotein provides insights into the cellular biology of a rhabdovirus in the insect vector.}, volume={577}, ISSN={["1089-862X"]}, DOI={10.1016/j.virol.2022.10.002}, abstractNote={Rhabdovirus glycoproteins (G) serve multifunctional roles in virus entry, assembly, and exit from animal cells. We hypothesize that maize mosaic virus (MMV) G is required for invasion, infection, and spread in Peregrinus maidis, the planthopper vector. Using a membrane-based yeast two-hybrid assay, we identified 107 P. maidis proteins that physically interacted with MMV G, of which approximately 53% matched proteins with known functions including endocytosis, vesicle-mediated transport, protein synthesis and turnover, nuclear export, metabolism and host defense. Physical interaction networks among conserved proteins indicated a possible cellular coordination of processes associated with MMV G translation, protein folding and trafficking. Non-annotated proteins contained predicted functional sites, including a diverse array of ligand binding sites. Cyclophilin A and apolipophorin III co-immunoprecipitated with MMV G, and each showed different patterns of localization with G in insect cells. This study describes the first protein interactome for a rhabdovirus spike protein and insect vector.}, journal={VIROLOGY}, author={Alviar, Karen B. and Rotenberg, Dorith and Martin, Kathleen M. and Whitfield, Anna E.}, year={2022}, month={Dec}, pages={163–173} }
 @article{martin_whitfield_2019, title={Complete Genome Sequence of Maize Mosaic Nucleorhabdovirus}, volume={8}, ISSN={["2576-098X"]}, DOI={10.1128/MRA.00637-19}, abstractNote={
            The complete genome sequence of maize mosaic virus (MMV) was obtained using next-generation sequencing from infected
            Peregrinus maidis
            and rapid amplification of cDNA ends from infected
            Zea mays
            . The genome of MMV is 12,170 bases, and this project completed the 5′ and 3′ ends and amended the polymerase sequence.
          }, number={29}, journal={MICROBIOLOGY RESOURCE ANNOUNCEMENTS}, author={Martin, Kathleen M. and Whitfield, Anna E.}, year={2019}, month={Jul} }
 @article{badillo-vargas_chen_martin_rotenberg_whitfield_2019, title={Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus}, volume={93}, ISSN={["1098-5514"]}, url={https://doi.org/10.1101/416560}, DOI={10.1128/JVI.00699-19}, abstractNote={
            Thrips-transmitted viruses cause devastating losses to numerous food crops worldwide. For negative-sense RNA viruses that infect plants, the arthropod serves as a host as well by supporting virus replication in specific tissues and organs of the vector. The goal of this work was to identify thrips proteins that bind directly to the viral attachment protein and thus may play a role in the infection cycle in the insect. Using the model plant bunyavirus tomato spotted wilt virus (TSWV), and the most efficient thrips vector, we identified and validated six TSWV-interacting proteins from
            Frankliniella occidentalis
            first-instar larvae. Two proteins, an endocuticle structural glycoprotein and cyclophilin, were able to interact directly with the TSWV attachment protein, G
            N
            , in insect cells. The TSWV G
            N
            -interacting proteins provide new targets for disrupting the viral disease cycle in the arthropod vector and could be putative determinants of vector competence.
          }, number={21}, journal={Journal of Virology}, author={Badillo-Vargas, I.E. and Chen, Y. and Martin, K.M. and Rotenberg, D. and Whitfield, A.E.}, year={2019}, pages={e00699–19} }
 @article{martin_whitfield_2018, title={Cellular localization and interactions of nucleorhabdovirus proteins are conserved between insect and plant cells}, volume={523}, ISSN={["0042-6822"]}, DOI={10.1016/j.virol.2018.06.019}, abstractNote={Maize mosaic virus (MMV), similar to other nucleorhabdoviruses, replicates in divergent hosts: plants and insects. To compare MMV protein localization and interactions, we visualized autofluorescent protein fusions in both cell types. Nucleoprotein (N) and glycoprotein (G) localized to the nucleus and cytoplasm, phosphoprotein (P) was only found in the nucleus, and 3 (movement) and matrix (M) were present in the cytoplasm. This localization pattern is consistent with the model of nucleorhabdoviral replication of N, P, L and viral RNA forming a complex in the nucleus and the subvirion associating with M and then G during budding into perinuclear space. The comparable localization patterns in both organisms indicates a similar replication cycle. Changes in localization when proteins were co-expressed suggested viral proteins interact thus altering organelle targeting. We documented a limited number of direct protein interactions indicating host factors play a role in the virus protein interactions during the infection cycle.}, journal={VIROLOGY}, author={Martin, Kathleen M. and Whitfield, Anna E.}, year={2018}, month={Oct}, pages={6–14} }
 @article{majee_kumar_kathare_wu_gingerich_nayak_salaita_dinkins_martin_goodin_et al._2018, title={KELCH F-BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME-INTERACTING FACTOR1}, volume={115}, ISSN={["0027-8424"]}, DOI={10.1073/pnas.1711919115}, abstractNote={Significance
          
            The completion of seed germination is an irrevocable event for plants, determining, for most plants, the site of the remainder of their life cycle. One environmental cue important to the completion of seed germination is light, which, in
            Arabidopsis thaliana
            , can influence a host of transcription factors, including PHYTOCHROME-INTERACTING FACTOR1 (PIF1), a negative regulator of the completion of germination and seedling de-etiolation. The KELCH F-BOX protein COLD TEMPERATURE GERMINATING10 (CTG10) can recognize and bind to PIF1, negatively influencing PIF1 stability, stimulating the completion of germination, and promoting a de-etiolated seedling morphology. PIF1, in turn, can downregulate
            CTG10
            expression, revealing a complex coregulation orchestrated by light presence and quality that dictates whether the seed completes germination.
          }, number={17}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Majee, Manoj and Kumar, Santosh and Kathare, Praveen Kumar and Wu, Shuiqin and Gingerich, Derek and Nayak, Nihar R. and Salaita, Louai and Dinkins, Randy and Martin, Kathleen and Goodin, Michael and et al.}, year={2018}, month={Apr}, pages={E4120–E4129} }
 @article{whitfield_huot_martin_kondo_dietzgen_2018, title={Plant rhabdoviruses-their origins and vector interactions}, volume={33}, ISSN={["1879-6265"]}, DOI={10.1016/j.coviro.2018.11.002}, abstractNote={Classical plant rhabdoviruses infect monocot and dicot plants, have unsegmented negative-sense RNA genomes and have been taxonomically classified in the genera Cytorhabdovirus and Nucleorhabdovirus. These viruses replicate in their hemipteran vectors and are transmitted in a circulative-propagative mode and virus infection persists for the life of the insect. Based on the discovery of numerous novel rhabdoviruses in arthropods during metagenomic studies and extensive phylogenetic analyses of the family Rhabdoviridae, it is hypothesized that plant-infecting rhabdoviruses are derived from insect viruses. Analyses of viral gene function in plants and insects is beginning to reveal conserved and unique biology for these plant viruses in the two diverse hosts. New tools for insect molecular biology and infectious clones for plant rhabdoviruses are increasing our understanding of the lifestyles of these viruses.}, journal={CURRENT OPINION IN VIROLOGY}, author={Whitfield, Anna E. and Huot, Ordom Brian and Martin, Kathleen M. and Kondo, Hideki and Dietzgen, Ralf G.}, year={2018}, month={Dec}, pages={198–207} }
 @article{martin_barandoc-alviar_schneweis_stewart_rotenberg_whitfield_2017, title={Transcriptomic response of the insect vector, Peregrinus maidis, to Maize mosaic rhabdovirus and identification of conserved responses to propagative viruses in hopper vectors}, volume={509}, ISSN={0042-6822}, url={http://dx.doi.org/10.1016/j.virol.2017.05.019}, DOI={10.1016/j.virol.2017.05.019}, abstractNote={Maize mosaic virus (MMV) is a plant-pathogenic rhabdovirus that is transmitted by the corn planthopper, Peregrinus maidis, in a propagative manner. P. maidis supports long-term MMV infections with no negative effects on insect performance. To elucidate whole-body transcriptome responses to virus infection, RNA-Seq was used to examine differential gene expression of virus-infected adult insects, and libraries were prepared from replicated groups of virus-exposed insects and non-exposed insects. From the 68,003 de novo-assembled transcripts, 144 were differentially-expressed (DE) during viral infection with comparable numbers up- and down-regulated. DE transcripts with similarity to genes associated with transposable elements (i.e., RNA-directed DNA polymerases) were enriched and may represent a mechanisim for modulating virus infection. Comparison of the P. maidis DE transcripts to published propagative virus-responsive transcript databases for two other hopper vectors revealed that 16% of the DE transcripts were shared across the three systems and may represent conserved responses to propagative viruses.}, journal={Virology}, publisher={Elsevier BV}, author={Martin, Kathleen M. and Barandoc-Alviar, Karen and Schneweis, Derek J. and Stewart, Catherine L. and Rotenberg, Dorith and Whitfield, Anna E.}, year={2017}, month={Sep}, pages={71–81} }