Works (6)

Updated: March 25th, 2024 08:09

2023 journal article

A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease


By: C. Schaaf n, K. Polkoff n, A. Carter n, A. Stewart n, B. Sheahan n, J. Freund n, J. Ginzel*, J. Snyder* ...

author keywords: APC mutation; colorectal cancer; intestine; Lgr5; porcine; stem cell; transgenic large animal
MeSH headings : Humans; Swine; Animals; Mice; In Situ Hybridization, Fluorescence; Intestines; Stem Cells; Ileum; Colon; Coleoptera; Green Fluorescent Proteins / genetics; Receptors, G-Protein-Coupled / genetics
TL;DR: This study validates ISC isolation in a new porcine Leucine Rich Repeat Containing G Protein‐Coupled Receptor 5 (LGR5) reporter line and demonstrates the use of these pigs as a novel colorectal cancer model. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 12, 2023

2022 journal article

Allogeneic and xenogeneic lymphoid reconstitution in a RAG2(-/-)IL2RG(y/-) severe combined immunodeficient pig: A preclinical model for intrauterine hematopoietic transplantation


By: R. Sper n, J. Proctor n, O. Lascina n, L. Guo n, K. Polkoff n, T. Kaeser n, S. Simpson n, L. Borst n ...

author keywords: severe combined immunodeficiency; pig; xenotransplantation; allotransplantation; hematopoietic; RAG2; IL2RG; transgenic
TL;DR: The data shows that the pig thymus provides a microenvironment conducive to engraftment, survival and development of human T-cells and provides evidence that the developing T-cell compartment can be populated to a significant extent by human cells in large animals. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 14, 2022

2022 article

Donor Age and Time in Culture Affect Dermal Fibroblast Contraction in an In Vitro Hydrogel Model

Detwiler, A., Polkoff, K., Gaffney, L., Freytes, D. O., & Piedrahita, J. A. (2022, August 4). TISSUE ENGINEERING PART A, Vol. 8.

By: A. Detwiler n, K. Polkoff n, L. Gaffney n, D. Freytes n & J. Piedrahita n

author keywords: cell proliferation; collagen; dermis; extracellular matrix; fibroblasts; hydrogels; skin; wound healing
MeSH headings : Adult; Infant, Newborn; Humans; Swine; Animals; Hydrogels / pharmacology; Reproducibility of Results; Wound Healing; Collagen / chemistry; Fibroblasts
TL;DR: Results show how cellular responses in a hydrogel scaffold differ based on donor age and time in culture in vitro, and suggest that consistency in the cellular component of bioengineered skin products could be beneficial in the biomanufacturing of consistent, reliable skin grafts and graft in vivo models. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 29, 2022

2022 journal article

LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis


By: K. Polkoff n, N. Gupta n, A. Green n, Y. Murphy n, J. Chung n, K. Gleason n, S. Simpson*, D. Walker n, B. Collins n, J. Piedrahita n

MeSH headings : Animals; Animals, Genetically Modified; Biomarkers / metabolism; Hair Follicle / metabolism; Humans; Morphogenesis; Receptors, G-Protein-Coupled / genetics; Receptors, G-Protein-Coupled / metabolism; Skin; Stem Cells / metabolism; Swine
TL;DR: It is demonstrated that LGR5 is a marker of hair follicle stem cells across species in homeostasis and development and the strong similarities and important differences in expression patterns, gene expression profiles, and developmental processes between species are reported. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: June 20, 2022

2021 journal article

Characterizing the Effects of Synergistic Thermal and Photo-Cross-Linking during Biofabrication on the Structural and Functional Properties of Gelatin Methacryloyl (GeIMA) Hydrogels


By: P. Chansoria n, S. Asif n, K. Polkoff n, J. Chung n, J. Piedrahita n & R. Shirwaiker n

author keywords: GelMA; thermo-reversible cross-linking; photo-cross-linking; biofabrication; tissue engineering; CryoSEM; qPCR
MeSH headings : Biocompatible Materials; Gelatin; Humans; Hydrogels; Methacrylates; Tissue Engineering
TL;DR: The finding that hydrogels processed at higher temperature possessed lower structural fidelity but resulted in more cell elongation and greater anisotropy over time demonstrate the significant impact of GelMA material formulation and processing conditions on the structural and functional properties of the hydrogel. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 29, 2021

2020 journal article

In Vitro Validation of Transgene Expression in Gene-Edited Pias Using CRISPR Transcriptional Activators

CRISPR JOURNAL, 3(5), 409–418.

By: K. Polkoff n, J. Chung n, S. Simpson*, K. Gleason n & J. Piedrahita n

MeSH headings : Animals; Animals, Genetically Modified / genetics; CRISPR-Associated Protein 9 / genetics; CRISPR-Associated Protein 9 / metabolism; Cells, Cultured; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Editing / methods; Gene Expression; Nuclear Transfer Techniques; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; Swine; Trans-Activators / genetics; Trans-Activators / metabolism; Transgenes
TL;DR: It is shown that CRISPR-dCas9 transcriptional activators can be used to validate functional transgene insertion in nonexpressing easily cultured cells such as fibroblasts and is a tool that could be used across disciplines and animal species to save time and resources by verifying expected outcomes of gene edits before generating live animals. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 24, 2020

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