@article{shearer_ranney_2013, title={Ploidy Levels and Relative Genome Sizes of Species, Hybrids, and Cultivars of Dogwood (Cornus spp.)}, volume={48}, ISSN={["2327-9834"]}, DOI={10.21273/hortsci.48.7.825}, abstractNote={Dogwoods (Cornus spp.) are valuable nursery crops grown as landscape plants throughout much of the world. Although there has been considerable work on breeding and selecting dogwoods, there is little information available on genome sizes (DNA content) and ploidy levels within the genus, particularly for specific clones and cultivars. Our objective was to conduct a survey of relative genome sizes and ploidy levels of dogwood taxa representative of the big-bracted, cornelian cherry, and dwarf dogwood clades. Flow cytometry was used to determine relative genome sizes and ploidy levels of 94 accessions of various species, hybrids, and cultivars. Traditional cytology was performed on root cells of representative taxa to calibrate genome sizes with ploidy level. All dogwood accessions tested were diploid with the exception of C. canadensis that was tetraploid and the hybrid cultivar C. KN30-8 that was triploid. Relative genome sizes varied by clade, subgenus, and species with 1Cx values ranging from 1.07 pg for C. canadensis to 5.08 pg for C. eydeana. Relative genome sizes were also valuable for confirming hybridity of interspecific crosses in cases in which parents varied substan- tially in relative genome size and hybrids were intermediate. A broad range of inter- specific hybrids was documented including C. capitata 3 florida, C. capitata 3 kousa, C. elliptica 3 florida, C. hongkongensis 3 florida, C. kousa 3 elliptica, and C. kousa 3 florida. These results provide further insights into the cytogenetics, reproductive biology, crossability, and relative genome sizes of dogwoods.}, number={7}, journal={HORTSCIENCE}, author={Shearer, Kimberly and Ranney, Thomas G.}, year={2013}, month={Jul}, pages={825–830} }