Works (414)
Updated: October 2nd, 2024 05:08
2024 journal article
Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring
BIOSENSORS & BIOELECTRONICS, 261.
author keywords: Single-chain fragment variable; DNA aptamer; SELEX; Electrochemical aptamer sensor; Bioprocess monitoring
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: June 22, 2024
2024 journal article
Development of Direct Electron Transfer-Type Extended Gate Field Effect Transistor Enzymatic Sensors for Metabolite Detection
ANALYTICAL CHEMISTRY, 96(10), 4076–4085.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 1, 2024
2024 journal article
Development of a glycated albumin sensor employing dual aptamer-based extended gate field effect transistors
BIOSENSORS & BIOELECTRONICS, 251.
author keywords: Glycated albumin; Extended -gate field effect transistor; Diabetes; Glycated protein; Aptamer
TL;DR:
Simple and rapid dual aptamer-based EGFET sensors to monitor GA through measuring GHSA and total HSA concentration, without the need for sample pretreatment, a mandatory step in the current standard of enzymatic GA monitoring are developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 8, 2024
2024 journal article
Development of an electrochemical impedance spectroscopy immunosensor for insulin monitoring employing pyrroloquinoline quinone as an ingestible redox probe
BIOSENSORS & BIOELECTRONICS, 250.
author keywords: Electrochemical impedance spectroscopy; Pyrroloquinoline quinone; Redox probe; Insulin; In -vivo
TL;DR:
This study adopted a unique approach, focusing on the use of ingestible materials, by exploring naturally occurring substances within the body, with a specific emphasis on pyrroloquinoline quinone (PQQ), offering a pathway for potential in vivo applications by combining PQQ as a redox probe with EIS measurements.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 28, 2024
2024 journal article
Development of closed bipolar electrode based L-lactate sensor employing quasi-direct electron transfer type enzyme with cyclic voltammetry
Biosensors and Bioelectronics.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: March 8, 2024
2024 journal article
Development of tetravalent antibody-enzyme complexes employing a lactate oxidase and the application to electrochemical immunosensors
BIOSENSORS & BIOELECTRONICS, 267.
author keywords: Antibody-enzyme complex; Lactate oxidase; Labeling enzyme; Homogeneous electrochemical detection; Variable domain of heavy chain of heavy chain; antibody; Epidermal growth factor receptor
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2024
2024 journal article
Development of the BioBattery: A novel enzyme fuel cell using a multicopper oxidase as an anodic enzyme
Biosensors and Bioelectronics.
Source: ORCID
Added: February 9, 2024
2024 review
Direct Electron Transfer-Type Oxidoreductases for Biomedical Applications
[Review of ]. ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 26, 357–382.
author keywords: biosensors; direct electron transfer; DET-type enzyme; continuous monitoring; glucose dehydrogenase; fructose dehydrogenase; energy harvesting
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 9, 2024
2024 journal article
Discovery of periplasmic solute binding proteins with specificity for ketone bodies: β-hydroxybutyrate binding proteins
PROTEIN SCIENCE, 33(7).
author keywords: solute binding protein; periplasmic binding protein; beta-hydroxybutyrate; molecular recognition; poly(hydroxyalkanoate); AlphaFold2; molecular dynamics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: June 15, 2024
2024 article
Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA-Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element
Komiya, E., Takamatsu, S., Miura, D., Tsukakoshi, K., Tsugawa, W., Sode, K., … Asano, R. (2024, January 31). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 1.
TL;DR:
A versatile DNA–protein covalent-linking patch (D-Pclip) for fabricating covalent and stoichiometric DNA–protein complexes that covalently connects any uracil-inserted DNA sequence and any SpyCatcher-fused protein stoichiometrically and has a high potential for various applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 1, 2024
2024 journal article
Levodopa: From Biological Significance to Continuous Monitoring
ACS Sensors.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: August 1, 2024
2024 journal article
Potential of Enzymatically Synthesized Hemozoin Analog as Th1 Cell Adjuvant
Nanomaterials.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: September 8, 2024
2024 journal article
Rapid and Convenient Single-Chain Variable Fragment-Employed Electrochemical C-Reactive Protein Detection System
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25(5).
author keywords: IgG-free immunosensors; single-chain variable fragment; antibody-enzyme complex; C-reactive protein; point-of-care testing
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 8, 2024
2024 journal article
The 2.5th generation enzymatic sensors based on the construction of quasi-direct electron transfer type NAD(P)-Dependent dehydrogenases
BIOSENSORS & BIOELECTRONICS, 255.
author keywords: NAD(P)-dependent dehydrogenases; Quasi-DET-Type enzymes; 2.5 th generation enzymatic sensors; beta-hydroxybutyrate dehydrogenase; Ketone sensor
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 1, 2024
2023 journal article
(Invited) A Biosensor Platform for on-Site Virus Visualization
ECS Meeting Abstracts.
TL;DR:
This work designed sequential enzymatic reactions using oxidase and peroxidase-based molecules to be occurred only on the viral surface of inactivated SARS-CoV-2 to enable on-site visualization of virus detection and visualization using a conventional smartphone camera.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 9, 2024
2023 journal article
Development of a Versatile Method to Construct Direct Electron Transfer-Type Enzyme Complexes Employing SpyCatcher/SpyTag System
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(3).
author keywords: biosensors; direct electron transfer; SpyCatcher; SpyTag; glucose dehydrogenase; d-amino acid oxidase; l-lactate oxidase; biomedical engineering
MeSH headings : Flavin-Adenine Dinucleotide / metabolism; Electrons; Glucose 1-Dehydrogenase / metabolism; Proteins / metabolism; Oxidation-Reduction
TL;DR:
Remarkably, the heme domain served as an electron acceptor from complexed enzymes by intramolecular electron transfer; consequently, all constructed CYTc-SC/ST-Enzyme complexes showed DET ability to the electrode, demonstrating the versatility of this method.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
13. Climate Action
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 18, 2023
2023 journal article
Development of an Anti-Idiotype Aptamer-Based Electrochemical Sensor for a Humanized Therapeutic Antibody Monitoring
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(6).
author keywords: aptamers; bevacizumab; biosensors; therapeutic monoclonal antibody; point-of-care testing
MeSH headings : Bevacizumab; Aptamers, Nucleotide / metabolism; Antibodies, Monoclonal; Oxidation-Reduction; Electrochemical Techniques / methods; Biosensing Techniques / methods
TL;DR:
An anti-idiotype aptamer-based electrochemical sensor for a humanized therapeutic antibody, bevacizumab, based on square wave voltammetry (SWV) is reported, able to monitor the target mAb within 30 min by employing the anti-IDiotype bivalent aptamer modified with a redox probe.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 11, 2023
2023 journal article
Development of interdigitated electrode based enzyme sensor for triglyceride monitoring using engineered oxygen insensitive glycerol 3-phosphate oxidase
Sensors and Actuators Reports.
Source: ORCID
Added: January 28, 2024
2023 journal article
Exploration of DNA Binding Proteins As a Versatile Tool for Fabrication of DNA-Protein Complexes and Its Application to Biosensing System
ECS Meeting Abstracts.
TL;DR:
The challenge to utilize DNA binding proteins with high DNA binding ability as a versatile tool suitable for the fabrication of DNA-protein complexes using the database to fabricate the stoichiometric and stable DNA-protein complex is introduced.
(via Semantic Scholar)
Source: ORCID
Added: February 9, 2024
2023 journal article
In vivo continuous monitoring of peptides and proteins: Challenges and opportunities
Applied Physics Reviews.
TL;DR:
In this review, several key challenges regarding the use of affinity-type biosensing elements to measure the concentration of peptides/proteins continuously in vivo are discussed and general translation toward in vivo continuous sensing of peptide/protein analytes is discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: January 28, 2024
2023 journal article
Surface area independent response of closed bipolar electrodes
Sensors and Actuators Reports.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: January 3, 2023
2022 journal article
A Thiol-reactive Phenazine Ethosulfate - A Novel Redox Mediator for Quasi-direct Electron-transfer-type Sensors
SENSORS AND MATERIALS, 34(6), 2105–2124.
author keywords: thiol-reactive phenazine ethosulfate; quasi-direct electron transfer; redox probe; enzyme modification; lactate oxidase; electrochemical enzyme sensor; biomedical engineering
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 27, 2022
2022 journal article
An Amine-Reactive Phenazine Ethosulfate (arPES)-A Novel Redox Probe for Electrochemical Aptamer-Based Sensor
SENSORS, 22(5).
author keywords: aptamer; redox probe; amine-reactive phenazine ethosulfate; electrochemical sensor
TL;DR:
The use of amine-reactive phenazine ethosulfate (arPES) for E-AB through its conjugation to the terminus of thrombin-binding aptamer revealed the potential application of arPES for a simultaneous multianalyte detection system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 25, 2022
2022 journal article
Development of a DNA aptamer that binds to the complementarity-determining region of therapeutic monoclonal antibody and affinity improvement induced by pH-change for sensitive detection
BIOSENSORS & BIOELECTRONICS, 203.
author keywords: DNA aptamer; Therapeutic antibody; Antibody-aptamer complex; Affinity improvement; Electrochemical biosensor
MeSH headings : Antibodies, Monoclonal; Antibody Affinity; Aptamers, Nucleotide / chemistry; Biosensing Techniques; Complementarity Determining Regions / chemistry; Complementarity Determining Regions / genetics; Humans; Hydrogen-Ion Concentration
TL;DR:
A novel anti-idiotype aptamer with extraordinary specificity against the anti-vascular endothelial growth factor therapeutic mAb, bevacizumab is developed with Structural analysis of the antibody-aptamer complex showed that several bases of A14#1 recognized only the complementarity determining region (CDR), thereby contributing to its extraordinary specificity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
16. Peace, Justice and Strong Institutions
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 11, 2022
2022 journal article
Development of a POCT type insulin sensor employing anti-insulin single chain variable fragment based on faradaic electrochemical impedance spectroscopy under single frequency measurement
Biosensors and Bioelectronics, 200, 113901.
author keywords: Insulin; Single chain variable fragment (scFv); Faradaic electrochemical impedance; spectroscopy; Point-of-care testing (POCT) sensor; Single frequency measurement
MeSH headings : Biosensing Techniques; Dielectric Spectroscopy; Electrochemical Techniques; Electrodes; Insulin; Point-of-Care Testing; Single-Chain Antibodies
TL;DR:
This study focuses on utilizing the anti-insulin single chain variable fragment (scFv) as a biosensing molecule with single-frequency faradaic electrochemical impedance spectroscopy (EIS) to aid in developing a future point-of-care sensor which rapidly and sensitively measures insulin across a dynamic range of physiological concentrations, with label-free detection.
(via Semantic Scholar)
Source: ORCID
Added: December 21, 2021
2022 journal article
Development of an electrochemical impedance spectroscopy based biosensor for detection of ubiquitin C-Terminal hydrolase L1
BIOSENSORS & BIOELECTRONICS, 208.
author keywords: Traumatic brain injury; Ubiquitin C-Terminal hydrolase L1 (UCH-L1); Electrochemical impedance spectroscopy; Point-of-care testing; Immunosensor
MeSH headings : Biomarkers; Biosensing Techniques; Brain Injuries, Traumatic / diagnosis; Dielectric Spectroscopy; Humans; Ubiquitin Thiolesterase
TL;DR:
This system could detect UCH-L1 within 5 min, without a washing step nor bound/free separation, and had resolution across concentrations ranging from 1 pM to 1000 pM within an artificial serum sample, which makes this platform an attractive candidate for scale-up as a device for rapid, on-site detection of TBI.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 30, 2022
2022 journal article
In Vitro Continuous 3 Months Operation of Direct Electron Transfer Type Open Circuit Potential Based Glucose Sensor: Heralding the Next CGM Sensor
JOURNAL OF DIABETES SCIENCE AND TECHNOLOGY, 16(5), 1107–1113.
author keywords: FADGDH; direct electron transfer (DET); 3(rd) generation glucose sensor; continuous glucose monitoring (CGM); Open circuit potential (OCP); long-term operation
MeSH headings : Humans; Biosensing Techniques / methods; Blood Glucose; Blood Glucose Self-Monitoring; Electrodes; Electrons; Flavin-Adenine Dinucleotide; Glucose; Gold
TL;DR:
The results suggest that the DET-type OCP glucose sensor is far more stable than the sensor constructed based on the amperometric principle, and is ideal for next generation CGM’s, especially in long duration implantable use cases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 27, 2022
2022 journal article
In Vitro Evaluation of Miniaturized Amperometric Enzyme Sensor Based on the Direct Electron Transfer Principle for Continuous Glucose Monitoring
Journal of Diabetes Science and Technology, 1, 193229682110706.
author keywords: FADGDH; direct electron transfer (DET); third-generation glucose sensor; continuous glucose monitoring (CGM)
MeSH headings : Humans; Acetaminophen; Biosensing Techniques / methods; Blood Glucose; Blood Glucose Self-Monitoring / methods; Electrodes; Electrons; Glucose; Glucose 1-Dehydrogenase
TL;DR:
The amperometric response of the sensor showed a linear response to glucose concentration up to 500 mg/dL without significant change of the response over an 11-day continuous measurement, indicating the superb potential of the DET-type FADGDH-based sensor with the combination of a miniaturized integrated electrode.
(via Semantic Scholar)
Source: ORCID
Added: January 7, 2022
2022 journal article
Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria
MICROBIAL CELL FACTORIES, 21(1).
author keywords: Biofuel; CcaS/CcaR two-component system; Cyanobacteria; Isobutanol; Light-regulated gene expression; Metabolic regulation; Synechocystis sp. PCC 6803; 3-methyl-1-butanol
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Butanols / metabolism; Light; Metabolic Engineering; Pentanols / metabolism; Photoreceptors, Microbial / genetics; Photoreceptors, Microbial / metabolism; Photosynthesis; Promoter Regions, Genetic; Synechocystis / genetics; Synechocystis / growth & development; Synechocystis / metabolism
TL;DR:
It is found that the production of isobutanol and 3MB from carbon dioxide (CO 2) was induced under red and green light illumination and was substantially repressed under red light illumination alone.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 18, 2022
2022 journal article
Microgravity environment grown crystal structure information based engineering of direct electron transfer type glucose dehydrogenase
COMMUNICATIONS BIOLOGY, 5(1).
MeSH headings : Glucose 1-Dehydrogenase; Blood Glucose Self-Monitoring; Electrons; Blood Glucose
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: December 7, 2022
2022 journal article
Structure of lactate oxidase from Enterococcus hirae revealed new aspects of active site loop function: Product-inhibition mechanism and oxygen gatekeeper
PROTEIN SCIENCE, 31(10).
author keywords: crystal structure; dehydrogenase; flavin; lactate oxidase; lactate sensor; l-lactate; oxidase; oxygen; product inhibition; substrate inhibition
MeSH headings : Catalytic Domain; Enterococcus hirae / metabolism; Flavin Mononucleotide / chemistry; Kinetics; Lactates; Ligands; Mixed Function Oxygenases / chemistry; Oxygen; Pyruvic Acid; Triose-Phosphate Isomerase / metabolism
TL;DR:
The structure analysis of EhLOx Met207Leu revealed the absence of flipping in the vicinity of FMN, unlike the wild‐type Met207 side chain, and the simulation of the oxygen‐accessible channel prediction, Met207 may play as an oxygen gatekeeper residue, which contributes oxygen uptake from external enzyme to FMN.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 22, 2022
2021 journal article
A self-powered glucose sensor based on BioCapacitor principle with micro-sized enzyme anode employing direct electron transfer type FADGDH
JOURNAL OF PHYSICS-ENERGY, 3(3).
author keywords: micro-sized electrode; biofuel cell; BioCapacitor; self-powered; glucose sensor; implantable artificial pancreas; direct electron transfer
TL;DR:
The development of a direct electron transfer type enzyme-based miniaturized self-powered glucose sensor based on the BioCapacitor principle with a micro-sized enzyme anode area indicates the potential to realize self- powered, autonomous, and implantable sensing modules for bio devices such as glucose-sensing systems for an artificial pancreas.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 14, 2021
2021 journal article
Artificial complementary chromatic acclimation gene expression system in Escherichia coli
MICROBIAL CELL FACTORIES, 20(1).
author keywords: Artificial complementary chromatic acclimation; Gene expression system; CcaS; CcaR; Escherichia coli
MeSH headings : Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Gene Expression Regulation, Bacterial; Genes, Reporter; Light; Luminescent Proteins / genetics; Phycobilins / biosynthesis; Phycobilins / genetics; Phycocyanin / biosynthesis; Phycocyanin / genetics; Promoter Regions, Genetic; Synechocystis / genetics; Synechocystis / metabolism; Transcription, Genetic; Transformation, Bacterial
TL;DR:
An artificial CCA system was developed to realize a multiple gene expression system, which was regulated by two colors, red and green lights, using a single photosensor system, the CcaS/CcaR system derived from Synechocystis sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: July 6, 2021
2021 journal article
Clinical Study of a High Accuracy Green Design Blood Glucose Monitor Using an Innovative Optical Transmission Absorbance System
JOURNAL OF DIABETES SCIENCE AND TECHNOLOGY, 16(5), 1069–1075.
author keywords: accuracy; blood glucose monitoring; green design; green diabetes initiative; optical BGM
MeSH headings : Blood Glucose; Blood Glucose Self-Monitoring; Data Collection; Diabetes Mellitus, Type 2; Humans; Hyperglycemia
TL;DR:
A new, highly accurate BGM system was developed based on the latest optical measurement system, introducing a rare metal-free “green-strip” that achieved the highest reported accuracy in clinical research, using venous blood from patients with diabetes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
9. Industry, Innovation and Infrastructure
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 30, 2023
2021 journal article
Continuous electrochemical monitoring of L-glutamine using redox-probe-modified L-glutamine-binding protein based on intermittent pulse amperometry
SENSORS AND ACTUATORS B-CHEMICAL, 346.
author keywords: L-glutamine; Continuous monitoring; L-glutamine-binding protein; Intermittent pulse amperometry; Electrochemical; Conformation change
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 5, 2021
2021 journal article
Continuous glucose monitoring systems-Current status and future perspectives of the flagship technologies in biosensor research
BIOSENSORS & BIOELECTRONICS, 181.
author keywords: Diabetes; Glucose sensor; Blood glucose monitoring; Continuous glucose monitoring; Artificial pancreas; Wearable biosensors; Green initiative
TL;DR:
Current and future technological aspects of CGM systems are introduced, the flagship technology in biosensor research, which was initiated, matured and is still growing in North America.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 24, 2021
2021 article
Data on G-quadruplex topology, and binding ability of G-quadruplex forming sequences found in the promoter region of biomarker proteins and those relations to the presence of nuclear localization signal in the proteins
Lee, J., Teramoto, K., Yokoyama, T., Ueno, K., Tsukakoshi, K., Sode, K., & Ikebukuro, K. (2021, June). DATA IN BRIEF, Vol. 36.
author keywords: G-quadruplex; Aptamer; Nuclear localization signal; Promoter region; Biomarker protein
TL;DR:
This data set summarized all the putative G4 forming sequences, their biochemical characteristics, and the presence of nuclear localization signal to address the possibility of binding of these PQS region to the target proteins in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 19, 2021
2021 journal article
Detection of Chloride Using Microelectrodes in Closed Bipolar Electrode Scheme
ECS Meeting Abstracts.
Source: ORCID
Added: November 12, 2021
2021 journal article
Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement
MOLECULES, 26(3).
author keywords: interdigitated electrode; glycated albumin; fructosyl amino acid oxidase; point of care testing; disposable enzyme sensor; diabetes mellitus
MeSH headings : Biosensing Techniques / instrumentation; Disposable Equipment; Electrodes; Enzymes / metabolism; Glycation End Products, Advanced; Reproducibility of Results; Serum Albumin / analysis; Glycated Serum Albumin
TL;DR:
It is demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 1, 2021
2021 journal article
Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase
BIOSENSORS & BIOELECTRONICS, 177.
author keywords: Haemoglobin A1c; Glycated protein; Fructosyl peptide oxidase; Flow injection analysis; Quasi-direct electron transfer; 2.5th generation principle
MeSH headings : Ascomycota; Biosensing Techniques; Electrons; Flow Injection Analysis; Glycated Hemoglobin / analysis; Humans; Peptides
TL;DR:
Results indicate that the enzyme electrochemical FIA system covers the clinical range of HbA1c detection for more 200 consecutive measurements, and will be possible to develop an integrated system consisting of sample pretreatment and sample electrochemical measurement based on an FIA system possessing quasi-DET type PnFPOx.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 15, 2021
2021 article
Editorial preface of the special issue on "the progress and perspectives of biosensing research in North America"
Li, C.-zhong, Liu, J., & Sode, K. (2021, December 15). BIOSENSORS & BIOELECTRONICS, Vol. 194.
MeSH headings : Biosensing Techniques; North America
Sources: Web Of Science, NC State University Libraries
Added: October 4, 2021
2021 journal article
Electrochemical quantification of accelerated FADGDH rates in aqueous nanodroplets
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 118(25).
author keywords: nanoelectrochemistry; nanodroplet; enzymology; FADGDH; reaction acceleration
MeSH headings : Electrochemistry; Electrodes; Flavin-Adenine Dinucleotide; Glucose Dehydrogenases / metabolism; Nanoparticles / chemistry; Water / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: June 16, 2021
2021 journal article
Electrochemical quantification of accelerated FADGDH rates in aqueous nanodroplets
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 118(25).
author keywords: nanoelectrochemistry; nanodroplet; enzymology; FADGDH; reaction acceleration
MeSH headings : Adolescent; Humans; United States / epidemiology; Child; Young Adult; Adult; Suicide, Attempted; Suicidal Ideation; Information Sources; Suicide Prevention; Surveys and Questionnaires; Risk Factors
Sources: Web Of Science, NC State University Libraries
Added: August 2, 2021
2021 journal article
G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol
NUCLEIC ACIDS RESEARCH, 49(11), 6069–6081.
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Computer Simulation; G-Quadruplexes; Heme / metabolism; Luminescence; Luminol / chemistry; Luminol / metabolism; Myoglobin / metabolism; Nuclear Magnetic Resonance, Biomolecular; Peroxidase / metabolism; SELEX Aptamer Technique; Substrate Specificity
TL;DR:
G-quadruplex (G4)-forming DNA aptamers that upregulate the peroxidase activity in myoglobin specifically for luminol are reported, which shows a parallel-type G4 structure with propeller-like loops that might be useful for a rational design of aptasensors utilizing the G4 aptamer-myoglobin pair.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 26, 2021
2021 journal article
Rapid, convenient, and highly sensitive detection of human hemoglobin in serum using a high-affinity bivalent antibody-enzyme complex
TALANTA, 234.
author keywords: Bivalent antibody-enzyme complex; Catcher/tag system; Glucose dehydrogenase; Hemoglobin; Homogeneous electrochemical detection; Single-chain variable fragment
MeSH headings : ErbB Receptors; Hemoglobins; Humans; Single-Chain Antibodies
TL;DR:
A rapid, convenient, and highly sensitive detection method for Hb in human serum using a bivalent antibody-enzyme complex (AEC) and a rapid and wash-free homogeneous electrochemical detection system for HB by integrating magnetic beads are established.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2021
2021 journal article
Strategic design and improvement of the internal electron transfer of heme b domain-fused glucose dehydrogenase for use in direct electron transfer-type glucose sensors
BIOSENSORS & BIOELECTRONICS, 176.
author keywords: Glucose dehydrogenase; Surface electrostatics; Direct electron transfer; Fusion protein; Docking simulation
MeSH headings : Biosensing Techniques; Blood Glucose; Blood Glucose Self-Monitoring; Electron Transport; Electrons; Flavin-Adenine Dinucleotide / metabolism; Glucose; Glucose 1-Dehydrogenase / metabolism; Heme; Saccharomycetales
TL;DR:
Results indicated that the sensor employed by the redesigned mutant Pcyb-AfGDH can be used for future continuous glucose monitoring system based on direct electron transfer principle.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 15, 2021
2021 journal article
Transient potentiometry based d-serine sensor using engineered d-amino acid oxidase showing quasi-direct electron transfer property
Biosensors and Bioelectronics, 200, 113927.
author keywords: D-Serine; Transient potentiometry; Open circuit potential; D-Amino acid Oxidase; Direct electron transfer
MeSH headings : Biosensing Techniques; Electrons; Flavin-Adenine Dinucleotide; Glucose; Glucose 1-Dehydrogenase; Potentiometry; Serine
TL;DR:
The transient potentiometry based sensing reported in this study will be further utilized to realize miniaturized, continuous, real-time, in vivo sensor for d-serine monitoring.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: December 30, 2021
2020 journal article
(Keynote) Continuous Glucose Monitoring Systems - Current Status and Future Perspectives of the Flagship Technologies in Biosensor Research -
ECS Meeting Abstracts, MA2020-02(44), 2777–2777.
Source: ORCID
Added: December 23, 2020
2020 journal article
A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria
Marine Biotechnology, 23(1), 31–38.
author keywords: T7 RNA polymerase; Green light-regulated gene expression system; Light-regulated bioprocesses; CcaS; CcaR; Synechocystissp; PCC6803; Cyanobacteria
MeSH headings : Bacterial Proteins; DNA-Directed RNA Polymerases; Gene Expression; Gene Expression Regulation, Bacterial / radiation effects; Genes, Reporter; Green Fluorescent Proteins / genetics; Light; Plasmids; Promoter Regions, Genetic; Synechocystis / genetics; Synechocystis / radiation effects; Viral Proteins
TL;DR:
Comparison with the strong promoter expression systems Pcpc560 and PtrcΔlacO revealed that the expression of sfGFP by the T7 RNAP system was comparable with the levels obtained with strong promoters.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Source: ORCID
Added: October 9, 2020
2020 review
Alteration of Electron Acceptor Preferences in the Oxidative Half-Reaction of Flavin-Dependent Oxidases and Dehydrogenases
[Review of ]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(11).
author keywords: oxidase; dehydrogenase; oxidative half-reaction; oxygen; electron acceptor; flavin adenine dinucleotide; flavin mononucleotide; oxygen accessible pathway; bioelectrochemistry; enzyme engineering
MeSH headings : Animals; Electron Transport; Flavins / chemistry; Flavins / metabolism; Flavoproteins / chemistry; Flavoproteins / metabolism; Humans; Oxidoreductases / chemistry; Oxidoreductases / metabolism; Oxygen / chemistry; Oxygen / metabolism
TL;DR:
In this review, recent progress in the engineering of the oxidative half-reaction of flavin-dependent oxidases and dehydrogenases is discussed, considering their current and future applications in bioelectrochemical studies, such as for the development of biosensors and biofuel cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 20, 2020
2020 journal article
Application of a Glucose Dehydrogenase-Fused with Zinc Finger Protein to Label DNA Aptamers for the Electrochemical Detection of VEGF
SENSORS, 20(14).
author keywords: aptamer; labeling; enzyme; zinc finger protein; glucose dehydrogenase; electrochemical sensor; vascular endothelial growth factor
MeSH headings : Aptamers, Nucleotide; Biosensing Techniques; Electrochemical Techniques; Glucose 1-Dehydrogenase; Humans; Vascular Endothelial Growth Factor A / analysis; Zinc Fingers
TL;DR:
This study used GDH that was genetically fused to a DNA binding protein, i.e., zinc finger protein (ZF), to demonstrate the concept of using ZF-fused GDH to enzymatically label aptamers for electrochemical detection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 24, 2020
2020 journal article
Construction of Super-Stabilized Direct Electron Transfer Type Glucose Dehydrogenase for Long Term Continuous Glucose Sensing System
ECS Meeting Abstracts, MA2020-02(44), 2831–2831.
Source: ORCID
Added: December 23, 2020
2020 journal article
Creation of a novel DET type FAD glucose dehydrogenase harboring Escherichia coli derived cytochrome b562 as an electron transfer domain
Biochemical and Biophysical Research Communications, 530(1), 82–86.
author keywords: Fusion enzymes; Direct electron transfer; Enzyme electrode; 3rd generation glucose sensor; Cytochrome b(562); FAD-Dependent glucose dehydrogenase
MeSH headings : Botrytis / genetics; Botrytis / metabolism; Cytochrome b Group / genetics; Cytochrome b Group / metabolism; Electron Transport; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Flavin-Adenine Dinucleotide / metabolism; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Protein Engineering; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Substrate Specificity
TL;DR:
The construction and production of a new DET-type FADGDH is demonstrated using E.coli as the host cells, which is advantageous for future industrial application and further engineering.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2020 journal article
Development of Insulin Sensors Based on Faradaic and Non-Faradaic Electrochemical Impedance Spectroscopy Detection Principle Using Single Chain Antibody (scFv)
ECS Meeting Abstracts, MA2020-02(44), 2783–2783.
Source: ORCID
Added: December 23, 2020
2020 journal article
Development of an Electrochemical Biosensor of Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) for the Assessment of Traumatic Brain Injury (TBI)
ECS Meeting Abstracts, MA2020-02(44), 2784–2784.
Source: ORCID
Added: December 23, 2020
2020 journal article
Electrochemical Aptasensor Employing Enzyme-Aptamer One-to-One Complex Showing Quasi-Direct Electron Transfer Ability
ECS Meeting Abstracts, MA2020-02(44), 2834–2834.
Source: ORCID
Added: December 23, 2020
2020 journal article
Engineered Glucose Oxidase Capable of Quasi-Direct Electron Transfer after a Quick-and-Easy Modification with a Mediator
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(3).
author keywords: glucose oxidase; direct electron transfer; amine-reactive phenazine ethosulfate; glucose sensor; glycemic level monitoring
MeSH headings : Aspergillus niger / enzymology; Biosensing Techniques / methods; Blood Glucose / analysis; Electrochemical Techniques; Fungal Proteins / metabolism; Glucose / metabolism; Glucose Oxidase / genetics; Glucose Oxidase / metabolism; Phenazines / metabolism; Protein Engineering
TL;DR:
E engineered GOx, which was made readily available for single-step modification with a redox mediator on its surface via a lysine residue rationally introduced into the enzyme, showed a quasi-DET response upon the addition of glucose.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 8, 2020
2020 journal article
Rational design of direct electron transfer type L-lactate dehydrogenase for the development of multiplexed biosensor
BIOSENSORS & BIOELECTRONICS, 176.
author keywords: L-lactate oxidase; Direct electron transfer; Fusion protein; Site-directed mutagenesis; Multiplexed biosensor; Biomedical engineering
MeSH headings : Biosensing Techniques; Blood Glucose; Blood Glucose Self-Monitoring; Electrodes; Electrons; Glucose; Glucose Oxidase; L-Lactate Dehydrogenase
TL;DR:
This is the first report of the DET-type enzyme based lactate and glucose dual sensing systems and the impact of the presence of electrochemical interferants was revealed to be negligible.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 8, 2021
2020 journal article
Rational engineering of Aerococcus viridans L-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor
BIOSENSORS & BIOELECTRONICS, 151.
author keywords: L-lactate oxidase; Amine reactive phenazine ethosulfate; Site-directed mutagenesis; Amperometric L-lactate biosensor; Interference; Direct electron transfer
MeSH headings : Aerococcus / chemistry; Aerococcus / isolation & purification; Biosensing Techniques; Catalysis; Enzymes, Immobilized / chemistry; Glucose / chemistry; Humans; Lactic Acid / chemistry; Lactic Acid / isolation & purification; Mixed Function Oxygenases / chemistry; Oxidation-Reduction
TL;DR:
Results indicated that a quasi-DET type lactate sensor was developed that did not suffer from the interference of oxygen and representative electroactive ingredient compounds.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 29, 2020
2020 journal article
The Abtamer: A Novel Antibody-Aptamer Conjugate for Electrochemical Biosensing Applications
ECS Meeting Abstracts, MA2020-02(44), 2835–2835.
Source: ORCID
Added: December 23, 2020
2020 journal article
The Continuous 3 Month Operation of Open Circuit Potential Based Glucose Sensor Employing Direct Electron Transfer Type Fad Dependent Glucose Dehydrogenase
ECS Meeting Abstracts, MA2020-02(44), 2779–2779.
Source: ORCID
Added: December 23, 2020
2019 journal article
Affinity sensor for haemoglobin A1c based on single-walled carbon nanotube field-effect transistor and fructosyl amino acid binding protein
BIOSENSORS & BIOELECTRONICS, 129, 254–259.
Contributors: M. Hatada*, T. Tran *, W. Tsugawa *, n & A. Mulchandani *
author keywords: Single-walled carbon nanotube; Field-effect transistor; Haemoglobin A1c; Fructosyl amino acid binding protein; SocA; Fructosyl valine
MeSH headings : Agrobacterium tumefaciens / chemistry; Bacterial Proteins / chemistry; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Equipment Design; Glycated Hemoglobin / analysis; Humans; Nanotubes, Carbon / chemistry; Proteolysis; Transistors, Electronic; Valine / analogs & derivatives; Valine / analysis
TL;DR:
The label-free affinity sensor for HbA1c is reported based on the chemiresistor-type field-effect transistor, which has the ability to measure as low as 1.2 nM FV, which is 14-fold more sensitive compared to the previously reported fluorescence-based sensor using SocA.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 20, 2020
2019 journal article
Application of an engineered chromatic acclimation sensor for red-light-regulated gene expression in cyanobacteria
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 44.
author keywords: Cyanobacteria; Marine cyanobacterium; Chromatic acclimation sensor; CcaS/CcaR two-component system; Miniaturized CcaS; Red-light-regulated gene expression
TL;DR:
The constructed red-light-regulated gene expression system using CcaS#11 has a great potential as a platform technology for the further development of light-regulated bioprocesses with strict control in cyanobacteria.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: December 16, 2019
2019 journal article
Designer fungus FAD glucose dehydrogenase capable of direct electron transfer
BIOSENSORS & BIOELECTRONICS, 123, 114–123.
Contributors: K. Ito *, J. Okuda-Shimazaki *, K. Mori*, K. Kojima *, W. Tsugawa *, K. Ikebukuro *, C. Lin *, J. La Belle *, H. Yoshida *, n
author keywords: Glucose dehydrogenase; Cellobiose dehydrogenase; Direct electron transfer; Designer FADGDH; Fusion protein; Heme b-binding cytochrome domain
MeSH headings : Aspergillus flavus / chemistry; Aspergillus flavus / enzymology; Biosensing Techniques; Blood Glucose / isolation & purification; Catalytic Domain; Electrodes; Electron Transport; Flavin-Adenine Dinucleotide / chemistry; Glucose Dehydrogenases / chemistry; Heme / chemistry
TL;DR:
The designer FADGDH was confirmed to be a novel GDH that possesses electrode DET ability and relevant and consistent findings provide a novel strategic approach for the improvement of the DET properties of designer FadGDH.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 20, 2020
2019 journal article
Development of a third-generation glucose sensor based on the open circuit potential for continuous glucose monitoring
BIOSENSORS & BIOELECTRONICS, 124, 216–223.
author keywords: Open circuit potential; Third generation glucose sensor; Continuous glucose monitoring (CGM); Direct electron transfer; FAD dependent glucose dehydrogenase (FADGDH)
MeSH headings : Biosensing Techniques; Blood Glucose / chemistry; Blood Glucose Self-Monitoring / methods; Diabetes Mellitus / blood; Diabetes Mellitus / pathology; Flavin-Adenine Dinucleotide / chemistry; Glucose / chemistry; Glucose / isolation & purification; Glucose 1-Dehydrogenase / chemistry; Humans; Insulin / chemistry; Insulin / metabolism
TL;DR:
The development of the third-generation type open circuit potential (OCP) principle-based glucose sensor with direct electron transfer FADGDH immobilized on gold electrodes using a self-assembled monolayer (SAM) is reported, and a novel algorithm for OCP- based glucose sensors is developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 31, 2018
2019 journal article
FAD dependent glucose dehydrogenases - Discovery and engineering of representative glucose sensing enzymes -
BIOELECTROCHEMISTRY, 132.
author keywords: Flavin adenine dinucleotide (FAD); Glucose dehydrogenase; Glucose sensor; Diabetes; Direct electron transfer (DET); Glucose oxidase
MeSH headings : Electrons; Flavin-Adenine Dinucleotide / metabolism; Fungi / enzymology; Glucose / metabolism; Glucose 1-Dehydrogenase / metabolism; Protein Engineering
TL;DR:
The two representative glucose sensing enzymes are focused on, by presenting their discovery, sources, and protein and enzyme properties, and the current engineering strategies to improve their potential in sensor applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 30, 2020
2019 journal article
G-Quadruplex Structure Improves the Immunostimulatory Effects of CpG Oligonucleotides
NUCLEIC ACID THERAPEUTICS, 29(4), 224–229.
author keywords: CpG ODN; toll-like receptor 9; G-quadruplex; IL-6; peripheral blood mononuclear cells
MeSH headings : Adjuvants, Immunologic / genetics; Adjuvants, Immunologic / pharmacology; Animals; Cell Line; CpG Islands / drug effects; G-Quadruplexes; Humans; Immunologic Factors / genetics; Leukocytes, Mononuclear / drug effects; Leukocytes, Mononuclear / immunology; Nucleic Acid Conformation / drug effects; Oligonucleotides, Antisense / chemistry; Oligonucleotides, Antisense / genetics; Phosphates / chemistry; Phosphates / metabolism
TL;DR:
Results indicate that G-quadruplex formation can be used to increase the immunostimulatory activity of CpG ODNs having a natural PD backbone.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 19, 2019
2019 journal article
Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase
BIOSENSORS & BIOELECTRONICS, 129, 189–197.
author keywords: Faradaic electrochemical impedance spectroscopy; Impedimetric biosensor; Direct electron transfer; FAD dependent glucose dehydrogenase complex; Charge transfer resistance; Imaginary impedance monitoring
MeSH headings : Bacteria / enzymology; Biosensing Techniques / methods; Electric Impedance; Electrodes; Electron Transport; Flavin-Adenine Dinucleotide / chemistry; Glucose / analysis; Glucose 1-Dehydrogenase / chemistry; Gold / chemistry
TL;DR:
The 3rd-generation faradaic enzyme EIS sensor would provide alternative platform for future impedimetric immunosensing system, which does not use redox probe, according to its potential application as the future immunosensor platform.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 11, 2019
2019 journal article
X-ray structure of the direct electron transfer-type FAD glucose dehydrogenase catalytic subunit complexed with a hitchhiker protein
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 75, 841–851.
author keywords: direct electron transfer; flavin adenine dinucleotide-dependent dehydrogenase complex; glucose dehydrogenase; iron-sulfur cluster; hitchhiker protein; glucose sensors; Burkholderia cepacia
MeSH headings : Burkholderia cepacia / enzymology; Catalytic Domain; Crystallography, X-Ray / methods; Electron Transport; Flavin-Adenine Dinucleotide / chemistry; Glucose Dehydrogenases / chemistry; Models, Molecular; Recombinant Proteins / chemistry
TL;DR:
The X-ray structure of the catalytic subunit of Burkholderia cepacia FAD glucose dehydrogenase complexed with a hitchhiker protein was determined as a representative molecule of direct electron transfer-type FAD-dependent dehydrogenases complexes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2019
2018 journal article
A Disposable Tear Glucose Biosensor-Part 5: Improvements in Reagents and Tear Sampling Component.
Journal of Diabetes Science and Technology, 12(4), 842–846.
Contributors: C. Lin*, Y. Ito*, A. Deng*, J. Johns*, D. Matloff*, C. Cook *, n , J. La Belle *
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Blood Glucose / analysis; Contact Lenses; Glucose 1-Dehydrogenase; Humans; Indicators and Reagents; Tears / chemistry
TL;DR:
A tear glucose (TG) sensor with an integrated tear sampler can provide a noninvasive method for calibrating the continuous TG contact lens and monitoring glucose and suggests that using enzymes with lower Km may be advantageous when operating in low glucose environments like tears.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2018 journal article
Biomolecular engineering of redox enzymes: Alteration of the electron acceptor preference; from oxygen to direct electron transfer
Abstracts of Papers of the American Chemical Society, 255. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000435537700584&KeyUID=WOS:000435537700584
Source: ORCID
Added: October 9, 2020
2018 journal article
Comprehensive study of domain rearrangements of single-chain bispecific antibodies to determine the best combination of configurations and microbial host cells
Mabs, 10(6), 854–863.
Contributors: R. Asano *, Y. Kuroki*, S. Honma, M. Akabane, S. Watanabe, S. Mayuzumi*, S. Hiyamuta*, I. Kumagai*, n
author keywords: Brevibacillus choshinensis; cancer immunotherapy; CD3; diabody; effective domain order; epidermal growth factor receptor; host cell; Pichia pastoris; small bispecific antibody; tandem scFv
MeSH headings : Antibodies, Bispecific / genetics; Antibodies, Bispecific / immunology; Binding Sites / genetics; Binding Sites / immunology; Brevibacillus / genetics; Cell Line, Tumor; Cell Survival / immunology; Electrophoresis, Polyacrylamide Gel; Escherichia coli / genetics; Genetic Variation; Humans; Pichia / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / immunology; Recombinant Fusion Proteins / metabolism; Single-Chain Antibodies / genetics; Single-Chain Antibodies / immunology
TL;DR:
This study designed all possible domain orders for scDb and taFv with identical Fv pairs and individually expressed all 16 variants using Escherichia coli, Pichia pastoris, and Brevibacillus choshinensis, finding a promising candidate that exhibited high activity and productivity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: October 9, 2020
2018 journal article
Construction and characterization of flavin adenine dinucleotide glucose dehydrogenase complex harboring a truncated electron transfer subunit
ELECTROCHIMICA ACTA, 277, 276–286.
Contributors: J. Okuda-Shimazaki *, N. Loew n, N. Hirose n, K. Kojima *, K. Mori*, W. Tsugawa *, n
author keywords: Direct electron transfer; Truncated electron transfer subunit; Heme c; Glucose dehydrogenase; Glucose sensing
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference
BIOELECTROCHEMISTRY, 121, 185–190.
Contributors: M. Hatada*, N. Loew n, Y. Inose-Takahashi*, J. Okuda-Shimazaki *, W. Tsugawa *, A. Mulchandani *, n
author keywords: 2.5th generation biosensor; Electrochemical enzyme sensor; Mediator modification of enzymes; Glucose dehydrogenase; Glucoside 3-dehydrogenase; Lactate oxidase
MeSH headings : Aerococcus / enzymology; Agrobacterium tumefaciens / enzymology; Biosensing Techniques / methods; Blood Glucose / analysis; Botrytis / enzymology; Electron Transport; Enzymes, Immobilized / chemistry; Glucose / analysis; Glucose 1-Dehydrogenase / chemistry; Glucose Dehydrogenases / chemistry; Humans; Mixed Function Oxygenases / chemistry; Phenazines / chemistry; Recombinant Proteins / chemistry
TL;DR:
Various kinds of diagnostic enzymes can achieve quasi-DET ability simply by modification with arPES, suggesting that continuous monitoring systems based on the 2.5th generation principle can be developed for various target molecules.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Development of an electrochemical detection system for CpG methylation of human genome using methyl CpG binding domain and zinc finger protein
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600001002&KeyUID=WOS:000447600001002
Contributors: J. Lee, W. Yoshida, D. Hiraoka, A. Tatsumi, K. Abe, K. Nakabayashi, H. Wakeda, K. Hata
Source: ORCID
Added: October 9, 2020
2018 journal article
Development toward a novel integrated tear lactate sensor using Schirmer test strip and engineered lactate oxidase
SENSORS AND ACTUATORS B-CHEMICAL, 270, 525–529.
Contributors: C. Lin *, K. Hiraka *, D. Matloff*, J. Johns*, A. Deng*, n , J. La Belle *
author keywords: Tear lactate; Schirmer's test strip; Integrated sensor; Lactate oxidase; Oxygen interference
TL;DR:
The proposed tear lactate (TL) sensor exhibited potential usefulness in providing an alternative noninvasive method of measuring lactate and in calibrating the continuous lactate contact lens.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Direct electron transfer (DET) mechanism of FAD dependent dehydrogenase complexes ∼from the elucidation of intra- and inter-molecular electron transfer pathway to the construction of engineered DET enzyme complexes∼
Current Opinion in Electrochemistry.
Contributors: Y. Yamashita *, I. Lee *, N. Loew n & n
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: October 9, 2020
2018 article
Elucidation of the intra- and inter-molecular electron transfer pathways of glucoside 3-dehydrogenase
Miyazaki, R., Yamazaki, T., Yoshimatsu, K., Kojima, K., Asano, R., Sode, K., & Tsugawa, W. (2018, August). BIOELECTROCHEMISTRY, Vol. 122, pp. 115–122.
Contributors: R. Miyazaki*, T. Yamazaki *, K. Yoshimatsu *, K. Kojima *, R. Asano *, n , W. Tsugawa *
author keywords: Glucoside 3 dehydrogenase; Electron transfer pathway; FAD; Fe-S cluster; Heme c
MeSH headings : Agrobacterium tumefaciens / chemistry; Agrobacterium tumefaciens / enzymology; Agrobacterium tumefaciens / genetics; Amino Acid Sequence; Catalytic Domain; Electron Transport; Genes, Bacterial; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Glucose Dehydrogenases / metabolism; Iron-Sulfur Proteins / chemistry; Iron-Sulfur Proteins / genetics; Iron-Sulfur Proteins / metabolism; Multigene Family; Sequence Alignment
TL;DR:
The results suggested that R. radiobacter G3DH possesses an iron‑sulfur cluster that may play an important role in the electron transfer from FAD to cytochrome c like molecule, which is an external electron acceptor of G3 DH.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Engineered fungus derived FAD-dependent glucose dehydrogenase with acquired ability to utilize hexaammineruthenium(III) as an electron acceptor
Bioelectrochemistry, 123, 62–69.
Contributors: M. Okurita*, N. Suzuki*, N. Loew n, H. Yoshida *, W. Tsugawa *, K. Mori*, K. Kojima *, D. Klonoff *, n
author keywords: Glucose dehydrogenase; Glucose oxidase; Flavin adenine dinucleotide; Aspergillus flavus; Hexaammineruthenium chloride; Enzyme sensor strip
MeSH headings : Amino Acid Substitution; Aspergillus flavus / enzymology; Aspergillus flavus / genetics; Aspergillus flavus / metabolism; Electrochemical Techniques; Electrons; Flavin-Adenine Dinucleotide / metabolism; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Models, Molecular; Protein Engineering; Ruthenium Compounds / metabolism
TL;DR:
A mutant of Aspergillus flavus-derived FADGDH, capable of utilizing hexaammineruthenium(III) as electron acceptor: AfGDH-H403D, which should lead to the development of sensor strips for blood glucose monitoring with increased accuracy and less stringent packing requirements.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: October 9, 2020
2018 journal article
Esterification of PQQ Enhances Blood-Brain Barrier Permeability and Inhibitory Activity against Amyloidogenic Protein Fibril Formation.
ACS Chemical Neuroscience.
Contributors: K. Tsukakoshi *, W. Yoshida *, M. Kobayashi *, N. Kobayashi *, J. Kim *, T. Kaku*, T. Iguchi*, K. Nagasawa *
author keywords: Pyrroloquinoline quinone trimethylester; alpha-synuclein; amyloid beta; blood-brain barrier permeability
MeSH headings : Amyloid / drug effects; Amyloid beta-Peptides / drug effects; Amyloid beta-Peptides / metabolism; Amyloidogenic Proteins / drug effects; Animals; Blood-Brain Barrier / metabolism; Cell Line, Tumor; Cell Survival / drug effects; Esterification; Esters / chemical synthesis; Esters / pharmacology; Humans; Mice; Neurons / drug effects; Neurons / metabolism; PQQ Cofactor / analogs & derivatives; PQQ Cofactor / chemical synthesis; PQQ Cofactor / pharmacology; Peptide Fragments / drug effects; Peptide Fragments / metabolism; Permeability; Prion Proteins / drug effects; Prion Proteins / metabolism; Protein Aggregation, Pathological / metabolism; alpha-Synuclein / drug effects; alpha-Synuclein / metabolism
TL;DR:
It is shown that an esterified compound of PQQ, P QQ-trimethylester (PQQ-TME), has twice the BBB permeability than PqQ in vitro, and indicated that esterification of PZQ could be a useful approach in developing a novel PQZ-based amyloid inhibitor.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2018 journal article
Glycogen Production in Marine Cyanobacterial Strain Synechococcus sp NKBG 15041c
Marine Biotechnology, 20(2), 109–117.
Contributors: A. Badary *, S. Takamatsu*, M. Nakajima*, S. Ferri *, P. Lindblad * & *
author keywords: Marine cyanobacteria; Synechococcus; Glycogen; Bioprocess; Carbohydrate production
MeSH headings : Biofuels; Glycogen / biosynthesis; Nitrogen / metabolism; Seawater; Synechococcus / genetics; Synechococcus / growth & development; Synechococcus / metabolism; Transcription, Genetic; Transcriptome
TL;DR:
The transcriptional analyses revealed that glycogen production in NKBG15041c under nitrogen-depleted conditions can be explained by the contribution of both increased carbon flux towards glycogen synthesis, similar to PCC6803 and PCC7002, and increased transcriptional levels of genes responsible for glycogens synthesis, resulting in a relatively high amount of glycogen under ambient conditions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2018 journal article
Improving the induction fold of riboregulators for cyanobacteria
Rna Biology, 15(3), 353–358.
Contributors: I. Sakamoto*, K. Abe *, S. Kawai*, K. Tsukakoshi *, Y. Sakai*, * , K. Ikebukuro *
author keywords: Cyanobacteria; post-transcriptional gene; regulation; riboregulator; RNA engineering; synthetic biology
MeSH headings : Gene Expression Regulation, Bacterial; Genetic Engineering / methods; Models, Molecular; Nucleic Acid Conformation; Promoter Regions, Genetic; RNA / chemistry; RNA / genetics; Synechocystis / genetics; Synechocystis / growth & development; Transcriptional Activation
TL;DR:
An improved riboregulator is improved by designing two RNA species, taRNA and crRNA, to enhance its induction fold and inserting nucleotides into the crRNA loop to enhance intermolecular hybridization and successfully improved its induction Fold.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2018 journal article
Minimizing the effects of oxygen interference on L-lactate sensors by a single amino acid mutation in Aerococcus viridans L-lactate oxidase
Biosensors & Bioelectronics, 103, 163–170.
Contributors: K. Hiraka *, K. Kojima *, C. Lin *, W. Tsugawa *, R. Asano *, J. La Belle *, *
author keywords: L-lactate oxidase; L-lactate; Oxygen; Screen-printed carbon electrode; Site-directed mutagenesis; Biomedical engineering
MeSH headings : Amino Acids / chemistry; Biosensing Techniques; Lactic Acid / chemistry; Lactic Acid / isolation & purification; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / genetics; Mutation; Oxidation-Reduction; Oxygen / chemistry
TL;DR:
This study provided significant insight into the enzymatic reaction mechanism of AvLOx and presented a novel approach to minimize oxygen interference in sensor applications, which will enable accurate detection of l-lactate concentrations.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2018 journal article
Mutagenesis Study of the Cytochrome c Subunit Responsible for the Direct Electron Transfer-Type Catalytic Activity of FAD-Dependent Glucose Dehydrogenase
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19(4).
Contributors: Y. Yamashita *, N. Suzuki*, N. Hirose*, K. Kojima *, W. Tsugawa * & n
author keywords: glucose dehydrogenase; direct electron transfer; cytochrome c; glucose sensor; biomedical engineering
MeSH headings : Amino Acid Sequence; Biocatalysis; Catalytic Domain / genetics; Cytochromes c / chemistry; Cytochromes c / genetics; Electrodes; Electrons; Enzyme Assays; Flavin-Adenine Dinucleotide / metabolism; Glucose 1-Dehydrogenase / metabolism; Mutagenesis / genetics; Mutant Proteins / metabolism; Solutions
TL;DR:
A mutagenesis investigation of the heme axial ligands was carried out to elucidate the electron transfer pathway to the electron mediators and/or the electrode, and two electron transfer pathways were proposed depending on the electron acceptors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Quasi-direct electron transfer-type glucose sensor based on engineered glucose oxidase
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600001209&KeyUID=WOS:000447600001209
Source: ORCID
Added: October 9, 2020
2018 journal article
Self-powered, wireless continuous glucose sensing system based direct electron transfer
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600000887&KeyUID=WOS:000447600000887
Source: ORCID
Added: October 9, 2020
2018 journal article
Smart aptamers forming G-quadruplex: Structural and functional change of aptamers forming G-quadruplex in response to surrounding conditions and its regulation with the ligands
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600001223&KeyUID=WOS:000447600001223
Source: ORCID
Added: October 9, 2020
2018 journal article
Synthesis of a hemin-containing copolymer as a novel immunostimulator that induces IFN-gamma production
International Journal of Nanomedicine, 13, 4461–4472.
Contributors: K. Hoshi*, T. Yamazaki *, C. Yoshikawa *, W. Tsugawa *, K. Ikebukuro * & *
author keywords: hemin copolymer; immunostimulator; adjuvant; radical polymerization; NIPAM; toll-like receptor 9; T-helper 1; inflammasome
MeSH headings : Acrylic Resins / chemical synthesis; Acrylic Resins / chemistry; Cell Death / drug effects; Cell Line; Hemin / pharmacology; Humans; Immunologic Factors / pharmacology; Interferon-gamma / biosynthesis; Interleukin-6 / biosynthesis; NF-kappa B / metabolism; Polymers / chemical synthesis; Polymers / chemistry; Signal Transduction / drug effects; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Toll-Like Receptors / metabolism
TL;DR:
It is found that NIPAM-hemin induced IL-6 and IFN-γ production in immune cells without any cytotoxic effects, and has potential therapeutic applications as a Th1-type adjuvant.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: October 9, 2020
2018 journal article
Wearable and implantable biosensing technologies based on the direct electron transfer principle
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600001124&KeyUID=WOS:000447600001124
Source: ORCID
Added: October 9, 2020
2017 journal article
Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp PCC 6803
Applied Microbiology and Biotechnology, 101(23-24), 8465–8474.
Contributors: K. Ueno *, Y. Sakai*, C. Shono*, I. Sakamoto*, K. Tsukakoshi *, Y. Hihara *, * , K. Ikebukuro *
author keywords: Riboregulator; Synechocystis sp PCC 6803; Cyanobacteria; cyabrB2; Gene regulation; Glycogen production
MeSH headings : Gene Expression Regulation, Bacterial; Genes, Regulator; Glycogen / biosynthesis; Metabolic Engineering / methods; Synechocystis / genetics; Synechocystis / metabolism; Transcription, Genetic
TL;DR:
Phenotypic similarities between the cyabrB2 expression-repressed mutant and the ΔcyabRB2 mutant suggest that the riboregulator can potentially be used as a new chromosomal gene regulation tool in cyanobacteria.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
Characterization of Electron Mediator Preference of Aerococcus viridans-Derived Lactate Oxidase for Use in Disposable Enzyme Sensor Strips
Sensors and Materials, 29(12), 1703–1711.
Contributors: N. Loew*, M. Fitriana *, K. Hiraka *, * & W. Tsugawa *
author keywords: lactate oxidase; screen-printed carbon electrode; electron mediator; 1-methoxy-5-methyl phenazinium methyl sulfate; potassium ferricyanide; hexaammineruthenium(III) chloride; biomedical engineering
TL;DR:
The protein scaffold of Av LOx can almost completely block the access to the co-factor for very small electron acceptors, depending on their charge.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
Continuous operation of an ultra-low-power microcontroller using glucose as the sole energy source
Biosensors & Bioelectronics, 93, 335–339.
Contributors: I. Lee *, T. Sode*, N. Loew*, W. Tsugawa *, C. Lowe * & *
author keywords: Microcontroller; BioCapacitor; Enzyme fuel cell; Direct electron transfer; Implantable artificial organs; Self-powered; Glucose sensor
MeSH headings : Artificial Organs; Bioelectric Energy Sources; Biosensing Techniques; Electrodes; Glucose / chemistry; Glucose 1-Dehydrogenase / chemistry; Humans
TL;DR:
The success in operating a microcontroller using glucose as the sole energy source indicated the probability of realizing implantable self-powered autonomously operated artificial organs, such as artificial pancreas.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2017 journal article
Development of a screen-printed carbon electrode based disposable enzyme sensor strip for the measurement of glycated albumin
Biosensors & Bioelectronics, 88, 167–173.
Contributors: M. Hatada*, W. Tsugawa *, E. Kamio*, N. Loew*, D. Klonoff * & *
author keywords: Diabetes mellitus; Glycated albumin; Fructosyl amino acid oxidase; Hemoglobin A1c; Point-of-care testing; Screen-printed carbon electrode
MeSH headings : Amino Acid Oxidoreductases / chemistry; Biosensing Techniques / instrumentation; Carbon / chemistry; Electrodes; Equipment Design; Glycation End Products, Advanced; Humans; Limit of Detection; Point-of-Care Systems; Reagent Strips / analysis; Serum Albumin / analysis; Glycated Serum Albumin
TL;DR:
The development of a sensing system for measuring GA by combining an enzyme analysis method, which is already used in clinical practice, with electrochemical principles is reported, which shows a clear correlation between the GA concentration and the resulting current.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
Mediator Preference of Two Different FAD-Dependent Glucose Dehydrogenases Employed in Disposable Enzyme Glucose Sensors
Sensors, 17(11).
Contributors: N. Loew*, W. Tsugawa *, D. Nagae*, K. Kojima * & *
author keywords: glucose dehydrogenase; flavin adenine dinucleotide; Aspergillus flavus; Burkholderia cepacia; enzyme sensor strip; mediated electron transfer; potassium ferricyanide; methoxy phenazine methosulfate; hexaammineruthenium chloride
MeSH headings : Biosensing Techniques; Flavin-Adenine Dinucleotide; Glucose / analysis; Glucose Dehydrogenases
TL;DR:
Results show that access of even very small mediators with strong charges to a buried active center can be almost completely blocked by the protein.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 chapter
Minimally invasive microneedle array electrodes employing direct electron transfer type glucose dehydrogenase for the development of continuous glucose monitoring sensors
In Biosensors 2016 (Vol. 27, pp. 208–209).
Contributors: S. Sharma *, E. Takagi*, T. Cass *, W. Tsugawa * & *
author keywords: continuous glucose monitoring; minimally invasive sensors; direct electron transfer; microneedles
TL;DR:
This work reports on the marriage of minimally invasive, continuous glucose sensors and a direct electron transfer type glucose dehydrogenase enzymatic system, which allows operation of the electrochemical sensor at lower potentials to minimize the effect of interference.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
Novel fungal FAD glucose dehydrogenase derived from Aspergillus niger for glucose enzyme sensor strips
Biosensors & Bioelectronics, 87, 305–311.
Contributors: * , N. Loew*, Y. Ohnishi*, H. Tsuruta*, K. Mori*, K. Kojima *, W. Tsugawa *, J. LaBelle *, D. Klonoff *
author keywords: FAD-dependent glucose dehydrogenase; Aspergillus niger; Aspergillus flavus; Glucose specificity; Xylose; BG Monitor
MeSH headings : Aspergillus niger / chemistry; Aspergillus niger / enzymology; Aspergillus niger / metabolism; Biosensing Techniques / methods; Flavin-Adenine Dinucleotide / metabolism; Glucose / analysis; Glucose / metabolism; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / metabolism; Humans; Models, Molecular; Substrate Specificity; Xylose / metabolism
TL;DR:
This is the first report of an enzyme sensor strip using a fungus derived FADGDH, which did not show any positive bias at a therapeutic level xylose concentration on the signal for a glucose sample, which clearly indicates the superiority of AnGDH over other conventionally used fungi derived F ADGDHs in the application for SMBG sensor strips.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
The electrochemical behavior of a FAD dependent glucose dehydrogenase with direct electron transfer subunit by immobilization on self-assembled monolayers
Bioelectrochemistry, 121, 1–6.
Contributors: I. Lee *, N. Loew*, W. Tsugawa *, C. Lin *, D. Probst *, J. La Belle *, n
MeSH headings : Biosensing Techniques / methods; Blood Glucose / analysis; Blood Glucose / metabolism; Burkholderia cepacia / enzymology; Electrochemical Techniques / methods; Electron Transport; Enzymes, Immobilized / metabolism; Flavin-Adenine Dinucleotide / metabolism; Glucose / analysis; Glucose / metabolism; Glucose 1-Dehydrogenase / metabolism; Humans
TL;DR:
The results indicate the feasibility of controlling the immobilization state of the enzymes on the electrode surface, and the current generated from DET-FADGDH electrodes decreased with increasing length of SAM, suggesting that the DET ability can be affected by the distance between the enzyme and the electrode.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018
2017 journal article
X-ray structures of fructosyl peptide oxidases revealing residues responsible for gating oxygen access in the oxidative half reaction
Scientific Reports, 7(1).
Contributors: T. Shimasaki *, H. Yoshida *, S. Kamitori * & *
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Substitution; Amino Acids / chemistry; Amino Acids / genetics; Amino Acids / metabolism; Binding Sites; Crystallography, X-Ray; Flavin-Adenine Dinucleotide / chemistry; Flavin-Adenine Dinucleotide / metabolism; Glycated Hemoglobin / metabolism; Models, Molecular; Mutagenesis, Site-Directed; Oxidation-Reduction; Oxygen / chemistry; Oxygen / metabolism; Protein Conformation; Structure-Activity Relationship
TL;DR:
Results reveal that Asn56 in PnFPOX is essential for maintaining an effective oxygen accession path, and support the role of Asp54 as a gate keeper that cooperates with Lys274 to enable oxygen to reach the active site properly.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2016 journal article
An Fe-S cluster in the conserved Cys-rich region in the catalytic subunit of FAD-dependent dehydrogenase complexes
Bioelectrochemistry, 112, 178–183.
Contributors: M. Shiota*, T. Yamazaki *, K. Yoshimatsu *, K. Kojima *, W. Tsugawa *, S. Ferri *, *
author keywords: Fe-S cluster; Glucose dehydrogenase; FAD; Direct electron transfer; FAD-dependent dehydrogenase
MeSH headings : Amino Acid Sequence; Catalytic Domain; Conserved Sequence; Cysteine; Flavin-Adenine Dinucleotide / metabolism; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Iron; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Sulfur
TL;DR:
Three Cys residues in the Cys-rich region constitute an iron-sulfur cluster that may play an important role in the electron transfer from FAD (intra-molecular) to the multi-heme cytochrome c subunit (inter-molescular) electron transfer pathway.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal
Scientific Reports, 6.
Contributors: M. Nakajima*, S. Ferri, M. Rogner * & *
MeSH headings : Acclimatization; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Cells, Cultured; Cyanobacteria / metabolism; Fluorescence; Gene Expression Regulation, Bacterial; Light Signal Transduction; Miniaturization / instrumentation; Models, Molecular; Protein Domains
TL;DR:
This study attempted to engineer the chromatic acclimation sensor CcaS, a CBCR derived from the cyanobacterium Synechocystis sp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Development of a light-regulated cell-recovery system for non-photosynthetic bacteria
Microbial Cell Factories, 15(1).
Contributors: M. Nakajima*, K. Abe *, S. Ferri * & *
author keywords: Antigen 43; Green light induction; Cell recovery; Green light sensor; Optogenetics; Escherichia coli
MeSH headings : Escherichia coli / cytology; Escherichia coli / radiation effects; Genetic Vectors / metabolism; Light; Photosynthesis / radiation effects; Plasmids / metabolism; Synechocystis / radiation effects; Time Factors; Transcription, Genetic / radiation effects
TL;DR:
This study demonstrated the construction of a cell recovery system for non-photosynthetic microorganisms induced by exposure of cells to green light and represents a novel tool for future bioprocessing with reduced energy and labor required for cell recovery.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Development of an electrochemical detection system for measuring DNA methylation levels using methyl CpG-binding protein and glucose dehydrogenase-fused zinc finger protein
Biosensors & Bioelectronics, 93, 118–123.
Contributors: J. Lee *, W. Yoshida *, K. Abe *, K. Nakabayashi *, H. Wakeda*, K. Hata *, C. Marquette *, L. Blum*, * , K. Ikebukuro *
author keywords: DNA methylation; Electrochemical detection; Diagnosis; Zinc finger protein; Glucose dehydrogenase; Methyl-CpG binding domain
MeSH headings : Biosensing Techniques; CpG Islands / genetics; DNA / chemistry; DNA Methylation / genetics; DNA-Binding Proteins / chemistry; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / genetics; Humans; Promoter Regions, Genetic
TL;DR:
A simple electrochemical detection system for DNA methylation using methyl CpG-binding domain (MBD) and a glucose dehydrogenase (GDH)-fused zinc finger protein is developed and the methylation levels at the promoter region of the androgen receptor gene are measured.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Electrochemical sensing system employing fructosamine 6-kinase enables glycated albumin measurement requiring no proteolytic digestion
Biotechnology Journal, 11(6), 797–804.
Contributors: M. Kameya*, W. Tsugawa *, M. Yamada-Tajima*, M. Hatada*, K. Suzuki *, A. Sakaguchi-Mikami*, S. Ferri *, D. Klonoff *, *
author keywords: Diabetes; Fructosamine 6-kinase; Glycated albumin; Glycated protein; Proteolytic digestion
MeSH headings : Biosensing Techniques / methods; Diabetes Mellitus / blood; Electrochemical Techniques / methods; Escherichia coli / enzymology; Escherichia coli Proteins / chemistry; Fructosamine / chemistry; Glycation End Products, Advanced; Humans; Oxidoreductases / chemistry; Phosphotransferases (Alcohol Group Acceptor) / chemistry; Proteolysis; Pyruvate Kinase / chemistry; Serum Albumin / analysis; Glycated Serum Albumin
TL;DR:
An electrochemical sensor based on fructosamine 6‐kinase (FN6K) from Escherichia coli, which can react directly with intact GA unlike FAOXs/FPOXs and could be applied to point‐of‐care‐testing devices for measurement of glycated proteins.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Structural regulation by a G-quadruplex ligand increases binding abilities of G-quadruplex-forming aptamers
Chemical Communications, 52(85), 12646–12649.
Contributors: K. Tsukakoshi *, Y. Ikuta*, K. Abe *, W. Yoshida *, K. Iida *, Y. Ma *, K. Nagasawa *, * , K. Ikebukuro *
TL;DR:
It is reported that a G-quadruplex-specific compound induced structural changes in G- Quadruple-forming DNA aptamers and stabilized the folded structures, making them suitable for targeted proteins and enhancing their binding properties.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
3RD GENERATION CGM SENSOR EMPLOYING DIRECT ELECTRON TRANSFER PRINCIPLE WITH IMPROVED OPERATIONAL CONDITIONS
Diabetes Technology & Therapeutics, 17, A90. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000349317500203&KeyUID=WOS:000349317500203
Source: ORCID
Added: October 9, 2020
2015 journal article
Advancing the development of glycated protein biosensing technology: next-generation sensing molecules.
Journal of Diabetes Science and Technology, 9(2), 183–191.
Contributors: M. Kameya*, A. Sakaguchi-Mikami*, S. Ferri *, W. Tsugawa * & *
MeSH headings : Amino Acid Oxidoreductases; Biomarkers / analysis; Biosensing Techniques / methods; Fructosamine; Humans
TL;DR:
Research on FN6K and fructosyl amino acid-binding protein has been opening up new possibilities for the development of highly sensitive and proteolytic-digestion-free biosensing systems for glycated proteins.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
BioCapacitor: A novel principle for biosensors
Biosensors & Bioelectronics, 76, 20–28.
Contributors: * , T. Yamazaki *, I. Lee *, T. Hanashi* & W. Tsugawa *
author keywords: BioCapacitor; Enzyme fuel cell; Charge pump; Implantable devices; Continuous glucose monitoring
MeSH headings : Biocatalysis; Bioelectric Energy Sources; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Electricity; Enzymes / chemistry; Glucose / isolation & purification; Glucose / metabolism; Humans
TL;DR:
The BioCapacitor principle, a novel biodevice based on an enzyme fuel cell that generates sufficient stable power to operate electric devices, is described and the recent challenges in the developments of self-powered stand-alone biodevices employing enzyme fuel cells combined with charge pumps and capacitor are introduced.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 article
Crystal structure of Aspergillus flavus FAD glucose dehydrogenase
Worldwide Protein Data Bank.
Source: ORCID
Added: October 9, 2020
2015 article
Crystal structure of Aspergillus flavus FADGDH in complex with D-glucono-1,5-lactone
Worldwide Protein Data Bank.
Source: ORCID
Added: October 9, 2020
2015 journal article
Development of an electrochemical adenosine diphosphate (ADP) sensing principle employing ADP-dependent kinase
Sensors and Materials, 27(9), 839–845.
Source: ORCID
Added: October 9, 2020
2015 journal article
Effects of Eliminating Pyruvate Node Pathways and of Coexpression of Heterogeneous Carboxylation Enzymes on Succinate Production by Enterobacter aerogenes
Applied and Environmental Microbiology, 81(3), 929–937.
Contributors: Y. Tajima*, Y. Yamamoto *, K. Fukui *, Y. Nishio*, K. Hashiguchi*, Y. Usuda *, *
MeSH headings : Actinobacillus / enzymology; Actinobacillus / genetics; Corynebacterium glutamicum / enzymology; Corynebacterium glutamicum / genetics; Culture Media / chemistry; Enterobacter aerogenes / enzymology; Enterobacter aerogenes / genetics; Enterobacter aerogenes / metabolism; Gene Deletion; Gene Expression; Glucose / metabolism; Hydrogen-Ion Concentration; Metabolic Engineering; Phosphoenolpyruvate Carboxykinase (ATP) / genetics; Phosphoenolpyruvate Carboxykinase (ATP) / metabolism; Pyruvate Carboxylase / genetics; Pyruvate Carboxylase / metabolism; Pyruvic Acid / metabolism; Succinic Acid / metabolism
TL;DR:
Lowering the pH in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs and can be applied to identify additional engineering targets to increase succinate production.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Efficient surface-display of autotransporter proteins in cyanobacteria
Algal Research-Biomass Biofuels and Bioproducts, 12, 337–340.
Contributors: S. Ferri *, M. Nakamura*, A. Ito *, M. Nakajima*, K. Abe *, K. Kojima *, *
author keywords: Cyanobacteria; Synechocystis; Autotransporter; Cell-surface display; Recombinant expression
TL;DR:
This is the first report of an efficient method for the display of recombinant proteins in cyanobacteria and the complete post-translational processing of the antigen 43 protein was confirmed by Western blot analysis.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Enzyme linking to DNA aptamers via a zinc finger as a bridge
Chemical Communications, 51(57), 11467–11469.
Contributors: K. Abe *, Y. Murakami *, A. Tatsumi*, K. Sumida*, A. Kezuka*, T. Fukaya*, T. Kumagai, Y. Osawa*, * , K. Ikebukuro *
MeSH headings : Animals; Aptamers, Nucleotide / chemistry; Biosensing Techniques; Cattle; Enzyme-Linked Immunosorbent Assay; Humans; Luciferases, Firefly / chemistry; Luminescent Agents / chemistry; Luminescent Measurements; Thrombin / analysis; Vascular Endothelial Growth Factor A / analysis; Zinc Fingers
TL;DR:
A novel enzyme-labeling method for DNA aptamers using enzyme-fused zinc finger proteins that achieved thrombin detection and vascular endothelial growth factor detection using zinc finger-fuse firefly luciferase is proposed.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in Enterobacter aerogenes
Microbial Cell Factories, 14(1), (11 June 2015).
Contributors: Y. Tajima*, Y. Yamamoto *, K. Fukui *, Y. Nishio*, K. Hashiguchi*, Y. Usuda *, *
author keywords: Enterobacter aerogenes; Succinate fermentation; Phosphoenolpyruvate carboxykinase; Glucose-PTS; Weak acidity; Anaerobic metabolism
MeSH headings : Adenosine Triphosphate / metabolism; Anaerobiosis; Culture Media / chemistry; Culture Media / metabolism; Enterobacter aerogenes / genetics; Enterobacter aerogenes / metabolism; Fermentation; Hydrogen-Ion Concentration; Metabolic Engineering / methods; Succinic Acid / metabolism
TL;DR:
Focusing on increasing net ATP production during succinate synthesis leads to increased succinate yield and volumetric productivity in E. aerogenes, demonstrating proof-of-concept that bacterium-based succinate fermentation can be improved under weakly acidic conditions.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Improvement of the VEGF binding ability of DNA aptamers through in silico maturation and multimerization strategy
Journal of Biotechnology, 212, 99–105.
Contributors: T. Fukaya*, K. Abe *, N. Savory *, K. Tsukakoshi *, W. Yoshida *, S. Ferri *, * , K. Ikebukuro *
author keywords: Aptamer; in silico maturation; Multimerization; Vascular endothelial growth factor
MeSH headings : Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / metabolism; Computer Simulation; Protein Binding; Protein Multimerization; Surface Plasmon Resonance; Vascular Endothelial Growth Factor A / genetics; Vascular Endothelial Growth Factor A / metabolism
TL;DR:
Improved DNA aptamer binding affinity for vascular endothelial growth factor (VEGF) through in silico maturation (ISM) and aptamer multimerization and designed SL5-trivalent aptamer with three binding motifs significantly increased binding affinity, representing a 500-fold improvement.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Klaus Mosbach Tribute
Biotechnology and Bioengineering, 112(4), 645–647.
Contributors: D. Clark *, I. Karube*, * , L. Bulow *, O. Brueggemann, C. Lowe *, P. Poulsen, R. Langer*
MeSH headings : Bioengineering / history; Bioengineering / methods; Biomedical Research / trends; Biosensing Techniques / methods; Biotechnology / history; Biotechnology / methods; History, 20th Century; History, 21st Century; Molecular Imprinting / methods
TL;DR:
Arevelatory theme of Klaus Mosbach’s work–thatbiomolecules and biopolymers could be controllably modified,manipulated, and assembled together in new ways, almost likeinterchangeablepartsofsomegrandmachine–was incrediblyexciting, andcontinued to driveriveinnovationsacrossmanydifferentdisciplines.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Scaffold-fused riboregulators for enhanced gene activation in Synechocystis sp PCC 6803
Microbiologyopen, 4(4), 533–540.
Contributors: Y. Sakai*, K. Abe *, S. Nakashima*, J. Ellinger *, S. Ferri *, * , K. Ikebukuro *
author keywords: Hfq; posttranscriptional gene regulation; riboregulator; Synechocystis sp PCC 6803; synthetic biology
MeSH headings : Gene Expression Regulation, Bacterial; Gene Targeting / methods; Molecular Biology / methods; Synechocystis / genetics; Synthetic Biology / methods; Transcriptional Activation
TL;DR:
The scaffold sequence derived from natural E. coli noncoding small RNAs is effective for designing RNA‐based genetic tools and scaffold‐fused riboregulators are a strong RNA‐tool to regulate gene expression in cyanobacteria.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Stabilization of fungi-derived recombinant FAD-dependent glucose dehydrogenase by introducing a disulfide bond
Biotechnology Letters, 37(5), 1091–1099.
author keywords: Aspergillus flavus; Disulfide bond; FAD; Glucose dehydrogenase; Site directed mutagenesis; Thermal stability
MeSH headings : Disulfides / chemistry; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Kinetics; Mutagenesis, Site-Directed; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Temperature
TL;DR:
The FAD-glucose dehydrogenase (GDH) mutant Val149Cys/Gly190Cys, which was constructed based on a comparison with the three dimensional structure of glucose oxidase showed a 110 min half-life of thermal inactivation at 45 °C, which is 13-fold greater than that of the wild-type enzyme.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Structural analysis of fungus-derived FAD glucose dehydrogenase
Scientific Reports, 5.
Contributors: H. Yoshida *, G. Sakai*, K. Mori*, K. Kojima *, S. Kamitori * & *
MeSH headings : Aspergillus / enzymology; Catalytic Domain; Crystallography, X-Ray; Flavin-Adenine Dinucleotide / metabolism; Gluconates / metabolism; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / metabolism; Histidine / chemistry; Hydrogen Bonding; Lactones / metabolism; Models, Molecular; Mutagenesis, Site-Directed; Oxygen / metabolism; Substrate Specificity
TL;DR:
The first three-dimensional structure of fungus-derived glucose dehydrogenase using flavin adenine dinucleotide (FAD) as the cofactor is reported, similar to that of fungal glucose oxidases (GOxs) reported till date.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
The Development and Characterization of an Exogenous Green-Light-Regulated Gene Expression System in Marine Cyanobacteria
Marine Biotechnology, 17(3), 245–251.
author keywords: Marine cyanobacteria; Synechococcus; Optogenetics; Green-light-sensing proteins; Synthetic biology
MeSH headings : Aquatic Organisms; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Gene Expression Regulation, Bacterial / radiation effects; Genes, Reporter; Genetic Engineering; Genetic Vectors / chemistry; Genetic Vectors / metabolism; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Histidine Kinase; Light; Promoter Regions, Genetic; Protein Kinases / genetics; Protein Kinases / metabolism; Real-Time Polymerase Chain Reaction; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Synechococcus / genetics; Synechococcus / metabolism; Synechococcus / radiation effects; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription, Genetic / radiation effects
TL;DR:
The constructed green-light-regulated gene expression system was effective at regulating the recombinant expression of a target gene using green light in a marine cyanobacterial strain that does not naturally possess such a green- light regulation system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2015 journal article
X-ray structure of recombinant nonglycosylated FAD glucose dehydrogenase derived from Aspergillus flavus
Febs Journal, 282, 279. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000362570605056&KeyUID=WOS:000362570605056
Source: ORCID
Added: October 9, 2020
2014 journal article
A green-light inducible lytic system for cyanobacterial cells
Biotechnology For Biofuels, 7(1), (9 April 2014).
Contributors: K. Miyake*, K. Abe *, S. Ferri *, M. Nakajima*, M. Nakamura*, W. Yoshida, K. Kojima *, K. Ikebukuro *, *
author keywords: Cyanobacteria; Self-lysis; Two-component system; T4 bacteriophage; Synthetic biology
TL;DR:
The constructed green-light inducible lytic system was clearly induced by green- light illumination, resulting in lytic cells that released intracellular phycocyanin into the culture supernatant, suggesting a future possibility to construct photosynthetic genetically modified organisms that are unable to survive under sunlight exposure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2014 journal article
Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein
Analytical Methods, 6(14), 4991–4994.
Contributors: J. Lee *, A. Tatsumi*, K. Abe *, W. Yoshida *, * & K. Ikebukuro *
TL;DR:
An electrochemical detection system for pathogenic bacteria by utilizing a glucose dehydrogenase-fused zinc finger protein (ZF-GDH), which could detect PCR products electrochemically without the need for DNA probe hybridization is developed.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2014 journal article
Engineering glucose oxidase to minimize the influence of oxygen on sensor response
Electrochimica Acta, 126, 158–161.
Contributors: Y. Horaguchi*, S. Saito *, K. Kojima *, W. Tsugawa *, S. Ferri * & *
author keywords: Biosensor; Glucose oxidase; Glucose dehydrogenase; Flavin adenine dinucleotide; Oxygen
TL;DR:
The rational amino acid substitution approach resulted in the construction of the Ser114Ala/Phe355Leu mutant, which has an 11-fold decrease in oxidase activity and 2.8-fold increase in dehydrogenase activity compared with wild-type GOx and was considerably less affected by oxygen than the wild- type GOx-based sensor at lower glucose concentrations.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2014 journal article
Study of the role of anaerobic metabolism in succinate production by Enterobacter aerogenes
Applied Microbiology and Biotechnology, 98(18), 7803–7813.
Contributors: Y. Tajima*, K. Kaida*, A. Hayakawa*, K. Fukui *, Y. Nishio*, K. Hashiguchi*, R. Fudou, K. Matsui*, Y. Usuda *, *
author keywords: Enterobacter aerogenes; Succinate fermentation; Redox balance; Phosphoenolpyruvate carboxykinase; Ethanol dehydrogenase
TL;DR:
These single-gene knockout studies showed that the ethanol synthesis pathway serves as the dominant NADH re-oxidation pathway in this organism and showed the potential of bacteria-based succinate fermentation under weak acidic conditions.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2014 journal article
THE 3RD GENERATION CGM SENSOR UTILIZING DIRECT ELECTRON TRANSFER PRINCIPLE
Diabetes Technology & Therapeutics, 16, A78–A79. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000331218000205&KeyUID=WOS:000331218000205
Source: ORCID
Added: October 9, 2020
2014 journal article
The development of an autonomous self-powered bio-sensing actuator
Sensors and Actuators B-Chemical, 196, 429–433.
Contributors: T. Hanashi*, T. Yamazaki *, H. Tanaka *, K. Ikebukuro *, W. Tsugawa * & *
author keywords: Enzyme fuel cell; Autonomous; Sensing actuator; Flavin adenine dinucleotide-dependent glucose dehydrogenase; Self-powered; Drug delivery systems
TL;DR:
An autonomous, self-powered, sensing actuator that employs the principle of BioCapacitor as the core technology is reported, which demonstrates its potential for application as a drug delivery/pumping system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2013 journal article
Blood Glucose Dependence on Emotional Behaviors and Body Surface Temperatures in Common Marmoset's Socio-Psychological Learning with Peers - for 'Development of Human-Environment Interface by Sensing and Multivariate Analysis of Bio-Ecosystem'
Bioengineering Based on Electrochemistry, 50(28), 9–14.
Contributors: M. Koshiba *, A. Senoo, G. Karino*, Y. Shirakawa*, K. Mimura *, T. Sagawa*, W. Tsugawa*, * , S. Nakamura *
Source: ORCID
Added: October 9, 2020
2013 journal article
Cloning and Characterization of Fructosamine-6-Kinase from Arthrobacter aurescens
Applied Biochemistry and Biotechnology, 170(3), 710–717.
Contributors: A. Sakaguchi-Mikami*, M. Kameya*, S. Ferri *, W. Tsugawa * & *
author keywords: Glycation; Amadori products; Arthrobacter; epsilon-Fructosyl lysine (epsilon-FK); Fructosamine-6-kinase (FN6K)
MeSH headings : Amino Acid Sequence; Arthrobacter / enzymology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Escherichia coli / genetics; Lysine / analogs & derivatives; Lysine / metabolism; Molecular Sequence Data; Phosphotransferases (Alcohol Group Acceptor) / genetics; Phosphotransferases (Alcohol Group Acceptor) / metabolism; Sequence Alignment; Substrate Specificity
TL;DR:
The presence of FN6K in Arthrobacter spp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Design of riboregulators for control of cyanobacterial (Synechocystis) protein expression
Biotechnology Letters, 36(2), 287–294.
Contributors: K. Abe *, Y. Sakai*, S. Nakashima*, M. Araki*, W. Yoshida *, * , K. Ikebukuro
author keywords: Biofuels; Cyanobacteria; Post-transcriptional gene regulation; Riboregulator; Synechocystis; Synthetic biology
MeSH headings : Bacterial Proteins / biosynthesis; Biotechnology / methods; Gene Expression Regulation, Bacterial; Metabolic Engineering / methods; Molecular Biology / methods; RNA / metabolism; Ribosomes / drug effects; Ribosomes / metabolism; Synechocystis / genetics; Synechocystis / metabolism
TL;DR:
R riboregulators, that one of the post-transcriptional regulators composed of RNAs, were constructed for controlling cyanobacterial protein expression and were effective in Synechocystis sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2013 journal article
Direct electron transfer type disposable sensor strip for glucose sensing employing an engineered FAD glucose dehydrogenase
Enzyme and Microbial Technology, 52(2), 123–128.
Contributors: Y. Yamashita *, S. Ferri *, M. Huynh*, H. Shimizu*, H. Yamaoka* & *
author keywords: Diabetes; Glucose dehydrogenase; Glucose sensor; SMBG; Substrate specificity; Direct electron transfer
MeSH headings : Amino Acid Sequence; Amino Acid Substitution; Aspergillus niger / enzymology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biosensing Techniques; Blood Glucose / analysis; Blood Glucose Self-Monitoring / instrumentation; Blood Glucose Self-Monitoring / methods; Burkholderia cepacia / enzymology; Burkholderia cepacia / genetics; Catalytic Domain; Computer Simulation; Electrochemical Techniques; Electron Transport; Escherichia coli; Flavin-Adenine Dinucleotide / metabolism; Fungal Proteins / metabolism; Glucose / metabolism; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Glucose Dehydrogenases / metabolism; Glucose Oxidase / metabolism; Humans; Maltose / metabolism; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Reagent Strips; Recombinant Proteins / metabolism; Sensitivity and Specificity; Substrate Specificity
TL;DR:
The engineered FADGDH was used to develop a direct electron transfer-type, disposable glucose sensor strip by immobilizing the enzyme complex onto a carbon screen-printed electrode and showed a clear glucose concentration-dependent response that was not affected by the presence of maltose.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Engineering Fructosyl Peptide Oxidase to Improve Activity Toward the Fructosyl Hexapeptide Standard for HbA1c Measurement
Molecular Biotechnology, 54(3), 939–943.
Contributors: S. Ferri *, Y. Miyamoto *, A. Sakaguchi-Mikami*, W. Tsugawa * & *
author keywords: Fructosyl peptide oxidase; Hemoglobin A1c; Glycated hemoglobin; Substrate specificity; Protein engineering; Dehydrogenase; Oxidase; Diabetes monitoring
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Sequence; Ascomycota / enzymology; Ascomycota / genetics; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Glycated Hemoglobin; Glycopeptides / chemistry; Glycopeptides / metabolism; Models, Molecular; Molecular Sequence Data; Mutation; Oligopeptides / chemistry; Oligopeptides / metabolism; Protein Engineering / methods; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Reference Standards; Substrate Specificity
TL;DR:
The recently discovered fructosyl peptide oxidase from Phaeosphaeria nodorum (PnFPOX) was demonstrated to react with the glycated hexapeptide measurement standard of hemoglobin A1c, fVHLTPE.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Engineering of a green-light inducible gene expression system in Synechocystis sp PCC6803
Microbial Biotechnology, 7(2), 177–183.
Contributors: K. Abe *, K. Miyake*, M. Nakamura*, K. Kojima *, S. Ferri *, K. Ikebukuro *, *
MeSH headings : Artificial Gene Fusion; Bacterial Proteins / genetics; Gene Expression; Gene Expression Regulation, Bacterial / radiation effects; Genes, Reporter; Genetic Engineering; Genetics, Microbial / methods; Green Fluorescent Proteins / analysis; Green Fluorescent Proteins / genetics; Light; Promoter Regions, Genetic; Synechocystis / genetics; Synechocystis / radiation effects; Transcriptional Activation / radiation effects
TL;DR:
The constructed engineered green‐light sensing system resulted in about 40‐fold higher protein expression than with the wild‐type promoter with a high ON/OFF ratio under green‐ light illumination, which would be a useful genetic tool for controlling gene expression in the emergent cyanobacterial bioprocesses.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Identification and functional analysis of fructosyl amino acid-binding protein from Gram-positive bacterium Arthrobacter sp.
Journal of Applied Microbiology, 114(5), 1449–1456.
Contributors: A. Sakaguchi-Mikami*, S. Ferri *, S. Katayama *, W. Tsugawa * & *
author keywords: Arthrobacter; fructosyl amino acid-binding protein; fructosyl amino acid; glycation; Gram-positive bacteria; Substrate-binding proteins
MeSH headings : Amino Acid Sequence; Arthrobacter / genetics; Arthrobacter / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Fatty Acids / metabolism; Fructosamine / metabolism; Ligands; Lipoproteins / genetics; Lipoproteins / metabolism; Membrane Proteins / genetics; Membrane Proteins / metabolism; Molecular Sequence Data; Protein Sorting Signals; Sequence Homology, Amino Acid; Substrate Specificity
TL;DR:
A novel FABP from Gram‐positive bacteria, Arthrobacter spp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2013 journal article
Improving the Gene-Regulation Ability of Small RNAs by Scaffold Engineering in Escherichia coli
Acs Synthetic Biology, 3(3), 152–162.
Contributors: Y. Sakai*, K. Abe *, S. Nakashima*, W. Yoshida *, S. Ferri *, * , K. Ikebukuro *
author keywords: attenuator; Hfq; post-transcriptional gene regulation; riboregulator; small RNA; transcriptional gene regulation
MeSH headings : Base Sequence; Escherichia coli / genetics; Gene Expression Regulation, Bacterial / genetics; Genetic Engineering / methods; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; RNA, Small Untranslated / genetics; Synthetic Biology / methods
TL;DR:
Efficient gene regulation abilities of small RNAs are improved by directly fusing the natural Escherichia coli trans-encoded small RNA-derived scaffolds, including Hfq-binding and rho-independent transcription terminator sequences, to the 3' end of the smallRNAs that mediate RNA-based gene regulation.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Mutational analysis of the oxygen-binding site of cholesterol oxidase and its impact on dye-mediated dehydrogenase activity
Journal of Molecular Catalysis B-Enzymatic, 88, 41–46.
Contributors: K. Kojima *, T. Kobayashi *, W. Tsugawa *, S. Ferri * & *
author keywords: Cholesterol dehydrogenase; Cholesterol oxidase; FAD; Site-directed mutagenesis
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2013 journal article
Partial Peptide of alpha-Synuclein Modified with Small-Molecule Inhibitors Specifically Inhibits Amyloid Fibrillation of alpha-Synuclein
International Journal of Molecular Sciences, 14(2), 2590–2600.
Contributors: W. Yoshida *, N. Kobayashi *, Y. Sasaki *, K. Ikebukuro * & *
author keywords: alpha-synuclein; amyloid beta; pyrroloquinoline quinone; Baicalein; EGCG
TL;DR:
These achievements provide a novel strategy for the development of targeted specific amyloid formation inhibitors: the combination of quinone compounds with specific peptide sequence from target proteins involved in amyloids formation.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Screening of Peptide Ligands for Pyrroloquinoline Quinone Glucose Dehydrogenase Using Antagonistic Template-Based Biopanning
International Journal of Molecular Sciences, 14(12), 23244–23256.
Contributors: K. Abe *, W. Yoshida *, K. Terada*, Y. Yagi-Ishii*, S. Ferri *, K. Ikebukuro *, *
author keywords: peptide ligand; phage display; site-directed; inhibitor