Works (428)
Updated: December 20th, 2024 14:35
2024 journal article
A Rapid, Homogeneous, and Universal Electrochemical Immunosensing System Using an Antibody/Split-Enzyme Complex
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 article
A Versatile Method to Create Antibody/Split-Enzyme Complexes and Its Application to a Rapid, Homogeneous, and Universal Electrochemical Immunosensing System
Tobita, Y., Hirano, K., Miura, D., Hatano, Y., Tsugawa, W., Ikebukuro, K., … Asano, R. (2024, October 7). ADVANCED SENSOR RESEARCH, Vol. 10.
author keywords: C-reactive protein; electrochemical sensors; homogeneous detection; single-chain fragment variables; split glucose dehydrogenases
open access via doi.org (publisher)
Sources: Web Of Science, NC State University Libraries
Added: October 14, 2024
2024 journal article
An electrochemical biosensor for the detection of tuberculosis specific DNA with CRISPR-Cas12a and redox-probe modified oligonucleotide
Heliyon.
Source: ORCID
Added: November 28, 2024
2024 journal article
Continuous Levodopa Sensor Employing Engineered Novel Direct Electron Transfer Type Enzyme
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Continuous Monitoring of Therapeutic Monoclonal Antibody Using Regenerable Aptamer Based Electrochemical Sensor
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Creation of Intrinsic Electrochemically-Active Solute Binding Proteins Suitable for Continuous Monitoring Systems
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Creation of pH-Sensitive Bioreceptors for Affinity-Type Electrochemical Biosensor Applications
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Development of Convenient Immunosensors Employing Single Chain Variable Fragment (scFv)-Displayed Biogenic Magnetic Nanoparticles
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring
BIOSENSORS & BIOELECTRONICS, 261.
author keywords: Single-chain fragment variable; DNA aptamer; SELEX; Electrochemical aptamer sensor; Bioprocess monitoring
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: June 22, 2024
2024 journal article
Development of Direct Electron Transfer-Type Extended Gate Field Effect Transistor Enzymatic Sensors for Metabolite Detection
ANALYTICAL CHEMISTRY, 96(10), 4076–4085.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 1, 2024
2024 journal article
Development of a Direct Electron Transfer-Type Enzymatic Sensor for the Spermine in Saliva, a Marker of Pancreatic Cancer
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Development of a glycated albumin sensor employing dual aptamer-based extended gate field effect transistors
BIOSENSORS & BIOELECTRONICS, 251.
author keywords: Glycated albumin; Extended -gate field effect transistor; Diabetes; Glycated protein; Aptamer
TL;DR:
Simple and rapid dual aptamer-based EGFET sensors to monitor GA through measuring GHSA and total HSA concentration, without the need for sample pretreatment, a mandatory step in the current standard of enzymatic GA monitoring are developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 8, 2024
2024 journal article
Development of an In Vivo, Real-Time and Continuous Insulin Sensor Utilizing an Extended Gate Field Effect Transistor-Based Transducer
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Development of an electrochemical impedance spectroscopy immunosensor for insulin monitoring employing pyrroloquinoline quinone as an ingestible redox probe
BIOSENSORS & BIOELECTRONICS, 250.
author keywords: Electrochemical impedance spectroscopy; Pyrroloquinoline quinone; Redox probe; Insulin; In -vivo
TL;DR:
This study adopted a unique approach, focusing on the use of ingestible materials, by exploring naturally occurring substances within the body, with a specific emphasis on pyrroloquinoline quinone (PQQ), offering a pathway for potential in vivo applications by combining PQQ as a redox probe with EIS measurements.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 28, 2024
2024 journal article
Development of closed bipolar electrode based L-lactate sensor employing quasi-direct electron transfer type enzyme with cyclic voltammetry
Biosensors and Bioelectronics.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: March 8, 2024
2024 journal article
Development of tetravalent antibody-enzyme complexes employing a lactate oxidase and the application to electrochemical immunosensors
BIOSENSORS & BIOELECTRONICS, 267.
author keywords: Antibody-enzyme complex; Lactate oxidase; Labeling enzyme; Homogeneous electrochemical detection; Variable domain of heavy chain of heavy chain; antibody; Epidermal growth factor receptor
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2024
2024 journal article
Development of the BioBattery: A novel enzyme fuel cell using a multicopper oxidase as an anodic enzyme
Biosensors and Bioelectronics.
Source: ORCID
Added: February 9, 2024
2024 review
Direct Electron Transfer-Type Oxidoreductases for Biomedical Applications
[Review of ]. ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 26, 357–382.
author keywords: biosensors; direct electron transfer; DET-type enzyme; continuous monitoring; glucose dehydrogenase; fructose dehydrogenase; energy harvesting
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 9, 2024
2024 journal article
Discovery of periplasmic solute binding proteins with specificity for ketone bodies: β-hydroxybutyrate binding proteins
PROTEIN SCIENCE, 33(7).
author keywords: solute binding protein; periplasmic binding protein; beta-hydroxybutyrate; molecular recognition; poly(hydroxyalkanoate); AlphaFold2; molecular dynamics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: June 15, 2024
2024 journal article
Electrochemical Sensing for HIV Integrase Activity Detection Employing G4/Hemin Dnazyme
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Elucidating the Mechanism of Electrochemical Inactivation of Direct Electron Transfer Type Glucose Dehydrogenase Enzyme-Employing Biosensor
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 article
Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA-Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element
Komiya, E., Takamatsu, S., Miura, D., Tsukakoshi, K., Tsugawa, W., Sode, K., … Asano, R. (2024, January 31). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 1.
TL;DR:
A versatile DNA–protein covalent-linking patch (D-Pclip) for fabricating covalent and stoichiometric DNA–protein complexes that covalently connects any uracil-inserted DNA sequence and any SpyCatcher-fused protein stoichiometrically and has a high potential for various applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 1, 2024
2024 journal article
Improvement of substrate specificity of the direct electron transfer type FAD-dependent glucose dehydrogenase catalytic subunit
JOURNAL OF BIOTECHNOLOGY, 395, 170–179.
author keywords: Glucose dehydrogenase; Direct electron transfer; Substrate specificity; X-ray structure; Galactose; Glucose sensors
Sources: Web Of Science, NC State University Libraries
Added: October 21, 2024
2024 journal article
Levodopa: From Biological Significance to Continuous Monitoring
ACS Sensors.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: August 1, 2024
2024 journal article
Potential of Enzymatically Synthesized Hemozoin Analog as Th1 Cell Adjuvant
Nanomaterials.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: September 8, 2024
2024 journal article
Proof-of-Concept Development of a Point-of-Care Direct Quantification of Glycated Albumin Using a Dual Electrochemical Method Approach
ECS Meeting Abstracts.
Source: ORCID
Added: December 19, 2024
2024 journal article
Rapid and Convenient Single-Chain Variable Fragment-Employed Electrochemical C-Reactive Protein Detection System
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25(5).
author keywords: IgG-free immunosensors; single-chain variable fragment; antibody-enzyme complex; C-reactive protein; point-of-care testing
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 8, 2024
2024 journal article
The 2.5th generation enzymatic sensors based on the construction of quasi-direct electron transfer type NAD(P)-Dependent dehydrogenases
BIOSENSORS & BIOELECTRONICS, 255.
author keywords: NAD(P)-dependent dehydrogenases; Quasi-DET-Type enzymes; 2.5 th generation enzymatic sensors; beta-hydroxybutyrate dehydrogenase; Ketone sensor
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 1, 2024
2023 journal article
(Invited) A Biosensor Platform for on-Site Virus Visualization
ECS Meeting Abstracts.
TL;DR:
This work designed sequential enzymatic reactions using oxidase and peroxidase-based molecules to be occurred only on the viral surface of inactivated SARS-CoV-2 to enable on-site visualization of virus detection and visualization using a conventional smartphone camera.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: February 9, 2024
2023 journal article
Development of a Versatile Method to Construct Direct Electron Transfer-Type Enzyme Complexes Employing SpyCatcher/SpyTag System
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(3).
author keywords: biosensors; direct electron transfer; SpyCatcher; SpyTag; glucose dehydrogenase; d-amino acid oxidase; l-lactate oxidase; biomedical engineering
MeSH headings : Flavin-Adenine Dinucleotide / metabolism; Electrons; Glucose 1-Dehydrogenase / metabolism; Proteins / metabolism; Oxidation-Reduction
TL;DR:
Remarkably, the heme domain served as an electron acceptor from complexed enzymes by intramolecular electron transfer; consequently, all constructed CYTc-SC/ST-Enzyme complexes showed DET ability to the electrode, demonstrating the versatility of this method.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
13. Climate Action
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 18, 2023
2023 journal article
Development of an Anti-Idiotype Aptamer-Based Electrochemical Sensor for a Humanized Therapeutic Antibody Monitoring
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(6).
author keywords: aptamers; bevacizumab; biosensors; therapeutic monoclonal antibody; point-of-care testing
MeSH headings : Bevacizumab; Aptamers, Nucleotide / metabolism; Antibodies, Monoclonal; Oxidation-Reduction; Electrochemical Techniques / methods; Biosensing Techniques / methods
TL;DR:
An anti-idiotype aptamer-based electrochemical sensor for a humanized therapeutic antibody, bevacizumab, based on square wave voltammetry (SWV) is reported, able to monitor the target mAb within 30 min by employing the anti-IDiotype bivalent aptamer modified with a redox probe.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 11, 2023
2023 journal article
Development of interdigitated electrode based enzyme sensor for triglyceride monitoring using engineered oxygen insensitive glycerol 3-phosphate oxidase
Sensors and Actuators Reports.
Source: ORCID
Added: January 28, 2024
2023 journal article
Exploration of DNA Binding Proteins As a Versatile Tool for Fabrication of DNA-Protein Complexes and Its Application to Biosensing System
ECS Meeting Abstracts.
TL;DR:
The challenge to utilize DNA binding proteins with high DNA binding ability as a versatile tool suitable for the fabrication of DNA-protein complexes using the database to fabricate the stoichiometric and stable DNA-protein complex is introduced.
(via Semantic Scholar)
Source: ORCID
Added: February 9, 2024
2023 journal article
In vivo continuous monitoring of peptides and proteins: Challenges and opportunities
Applied Physics Reviews.
TL;DR:
In this review, several key challenges regarding the use of affinity-type biosensing elements to measure the concentration of peptides/proteins continuously in vivo are discussed and general translation toward in vivo continuous sensing of peptide/protein analytes is discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: January 28, 2024
2023 journal article
Surface area independent response of closed bipolar electrodes
Sensors and Actuators Reports.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: January 3, 2023
2022 journal article
A Thiol-reactive Phenazine Ethosulfate - A Novel Redox Mediator for Quasi-direct Electron-transfer-type Sensors
SENSORS AND MATERIALS, 34(6), 2105–2124.
author keywords: thiol-reactive phenazine ethosulfate; quasi-direct electron transfer; redox probe; enzyme modification; lactate oxidase; electrochemical enzyme sensor; biomedical engineering
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 27, 2022
2022 journal article
An Amine-Reactive Phenazine Ethosulfate (arPES)-A Novel Redox Probe for Electrochemical Aptamer-Based Sensor
SENSORS, 22(5).
author keywords: aptamer; redox probe; amine-reactive phenazine ethosulfate; electrochemical sensor
TL;DR:
The use of amine-reactive phenazine ethosulfate (arPES) for E-AB through its conjugation to the terminus of thrombin-binding aptamer revealed the potential application of arPES for a simultaneous multianalyte detection system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 25, 2022
2022 journal article
Development of a DNA aptamer that binds to the complementarity-determining region of therapeutic monoclonal antibody and affinity improvement induced by pH-change for sensitive detection
BIOSENSORS & BIOELECTRONICS, 203.
author keywords: DNA aptamer; Therapeutic antibody; Antibody-aptamer complex; Affinity improvement; Electrochemical biosensor
MeSH headings : Antibodies, Monoclonal; Antibody Affinity; Aptamers, Nucleotide / chemistry; Biosensing Techniques; Complementarity Determining Regions / chemistry; Complementarity Determining Regions / genetics; Humans; Hydrogen-Ion Concentration
TL;DR:
A novel anti-idiotype aptamer with extraordinary specificity against the anti-vascular endothelial growth factor therapeutic mAb, bevacizumab is developed with Structural analysis of the antibody-aptamer complex showed that several bases of A14#1 recognized only the complementarity determining region (CDR), thereby contributing to its extraordinary specificity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
16. Peace, Justice and Strong Institutions
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 11, 2022
2022 journal article
Development of an electrochemical impedance spectroscopy based biosensor for detection of ubiquitin C-Terminal hydrolase L1
BIOSENSORS & BIOELECTRONICS, 208.
author keywords: Traumatic brain injury; Ubiquitin C-Terminal hydrolase L1 (UCH-L1); Electrochemical impedance spectroscopy; Point-of-care testing; Immunosensor
MeSH headings : Biomarkers; Biosensing Techniques; Brain Injuries, Traumatic / diagnosis; Dielectric Spectroscopy; Humans; Ubiquitin Thiolesterase
TL;DR:
This system could detect UCH-L1 within 5 min, without a washing step nor bound/free separation, and had resolution across concentrations ranging from 1 pM to 1000 pM within an artificial serum sample, which makes this platform an attractive candidate for scale-up as a device for rapid, on-site detection of TBI.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 30, 2022
2022 journal article
In Vitro Continuous 3 Months Operation of Direct Electron Transfer Type Open Circuit Potential Based Glucose Sensor: Heralding the Next CGM Sensor
JOURNAL OF DIABETES SCIENCE AND TECHNOLOGY, 16(5), 1107–1113.
author keywords: FADGDH; direct electron transfer (DET); 3(rd) generation glucose sensor; continuous glucose monitoring (CGM); Open circuit potential (OCP); long-term operation
MeSH headings : Humans; Biosensing Techniques / methods; Blood Glucose; Blood Glucose Self-Monitoring; Electrodes; Electrons; Flavin-Adenine Dinucleotide; Glucose; Gold
TL;DR:
The results suggest that the DET-type OCP glucose sensor is far more stable than the sensor constructed based on the amperometric principle, and is ideal for next generation CGM’s, especially in long duration implantable use cases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 27, 2022
2022 journal article
In Vitro Evaluation of Miniaturized Amperometric Enzyme Sensor Based on the Direct Electron Transfer Principle for Continuous Glucose Monitoring
Journal of Diabetes Science and Technology, 1, 193229682110706.
author keywords: FADGDH; direct electron transfer (DET); third-generation glucose sensor; continuous glucose monitoring (CGM)
MeSH headings : Humans; Acetaminophen; Biosensing Techniques / methods; Blood Glucose; Blood Glucose Self-Monitoring / methods; Electrodes; Electrons; Glucose; Glucose 1-Dehydrogenase
TL;DR:
The amperometric response of the sensor showed a linear response to glucose concentration up to 500 mg/dL without significant change of the response over an 11-day continuous measurement, indicating the superb potential of the DET-type FADGDH-based sensor with the combination of a miniaturized integrated electrode.
(via Semantic Scholar)
Source: ORCID
Added: January 7, 2022
2022 journal article
Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria
MICROBIAL CELL FACTORIES, 21(1).
author keywords: Biofuel; CcaS/CcaR two-component system; Cyanobacteria; Isobutanol; Light-regulated gene expression; Metabolic regulation; Synechocystis sp. PCC 6803; 3-methyl-1-butanol
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Butanols / metabolism; Light; Metabolic Engineering; Pentanols / metabolism; Photoreceptors, Microbial / genetics; Photoreceptors, Microbial / metabolism; Photosynthesis; Promoter Regions, Genetic; Synechocystis / genetics; Synechocystis / growth & development; Synechocystis / metabolism
TL;DR:
It is found that the production of isobutanol and 3MB from carbon dioxide (CO 2) was induced under red and green light illumination and was substantially repressed under red light illumination alone.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 18, 2022
2022 journal article
Microgravity environment grown crystal structure information based engineering of direct electron transfer type glucose dehydrogenase
COMMUNICATIONS BIOLOGY, 5(1).
MeSH headings : Glucose 1-Dehydrogenase; Blood Glucose Self-Monitoring; Electrons; Blood Glucose
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: December 7, 2022
2022 journal article
Structure of lactate oxidase from Enterococcus hirae revealed new aspects of active site loop function: Product-inhibition mechanism and oxygen gatekeeper
PROTEIN SCIENCE, 31(10).
author keywords: crystal structure; dehydrogenase; flavin; lactate oxidase; lactate sensor; l-lactate; oxidase; oxygen; product inhibition; substrate inhibition
MeSH headings : Catalytic Domain; Enterococcus hirae / metabolism; Flavin Mononucleotide / chemistry; Kinetics; Lactates; Ligands; Mixed Function Oxygenases / chemistry; Oxygen; Pyruvic Acid; Triose-Phosphate Isomerase / metabolism
TL;DR:
The structure analysis of EhLOx Met207Leu revealed the absence of flipping in the vicinity of FMN, unlike the wild‐type Met207 side chain, and the simulation of the oxygen‐accessible channel prediction, Met207 may play as an oxygen gatekeeper residue, which contributes oxygen uptake from external enzyme to FMN.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 22, 2022
2021 journal article
A self-powered glucose sensor based on BioCapacitor principle with micro-sized enzyme anode employing direct electron transfer type FADGDH
JOURNAL OF PHYSICS-ENERGY, 3(3).
author keywords: micro-sized electrode; biofuel cell; BioCapacitor; self-powered; glucose sensor; implantable artificial pancreas; direct electron transfer
TL;DR:
The development of a direct electron transfer type enzyme-based miniaturized self-powered glucose sensor based on the BioCapacitor principle with a micro-sized enzyme anode area indicates the potential to realize self- powered, autonomous, and implantable sensing modules for bio devices such as glucose-sensing systems for an artificial pancreas.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 14, 2021
2021 journal article
Artificial complementary chromatic acclimation gene expression system in Escherichia coli
MICROBIAL CELL FACTORIES, 20(1).
author keywords: Artificial complementary chromatic acclimation; Gene expression system; CcaS; CcaR; Escherichia coli
MeSH headings : Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Gene Expression Regulation, Bacterial; Genes, Reporter; Light; Luminescent Proteins / genetics; Phycobilins / biosynthesis; Phycobilins / genetics; Phycocyanin / biosynthesis; Phycocyanin / genetics; Promoter Regions, Genetic; Synechocystis / genetics; Synechocystis / metabolism; Transcription, Genetic; Transformation, Bacterial
TL;DR:
An artificial CCA system was developed to realize a multiple gene expression system, which was regulated by two colors, red and green lights, using a single photosensor system, the CcaS/CcaR system derived from Synechocystis sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: July 6, 2021
2021 journal article
Clinical Study of a High Accuracy Green Design Blood Glucose Monitor Using an Innovative Optical Transmission Absorbance System
JOURNAL OF DIABETES SCIENCE AND TECHNOLOGY, 16(5), 1069–1075.
author keywords: accuracy; blood glucose monitoring; green design; green diabetes initiative; optical BGM
MeSH headings : Blood Glucose; Blood Glucose Self-Monitoring; Data Collection; Diabetes Mellitus, Type 2; Humans; Hyperglycemia
TL;DR:
A new, highly accurate BGM system was developed based on the latest optical measurement system, introducing a rare metal-free “green-strip” that achieved the highest reported accuracy in clinical research, using venous blood from patients with diabetes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
9. Industry, Innovation and Infrastructure
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 30, 2023
2021 journal article
Continuous electrochemical monitoring of L-glutamine using redox-probe-modified L-glutamine-binding protein based on intermittent pulse amperometry
SENSORS AND ACTUATORS B-CHEMICAL, 346.
author keywords: L-glutamine; Continuous monitoring; L-glutamine-binding protein; Intermittent pulse amperometry; Electrochemical; Conformation change
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 5, 2021
2021 journal article
Continuous glucose monitoring systems-Current status and future perspectives of the flagship technologies in biosensor research
BIOSENSORS & BIOELECTRONICS, 181.
author keywords: Diabetes; Glucose sensor; Blood glucose monitoring; Continuous glucose monitoring; Artificial pancreas; Wearable biosensors; Green initiative
TL;DR:
Current and future technological aspects of CGM systems are introduced, the flagship technology in biosensor research, which was initiated, matured and is still growing in North America.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 24, 2021
2021 article
Data on G-quadruplex topology, and binding ability of G-quadruplex forming sequences found in the promoter region of biomarker proteins and those relations to the presence of nuclear localization signal in the proteins
Lee, J., Teramoto, K., Yokoyama, T., Ueno, K., Tsukakoshi, K., Sode, K., & Ikebukuro, K. (2021, June). DATA IN BRIEF, Vol. 36.
author keywords: G-quadruplex; Aptamer; Nuclear localization signal; Promoter region; Biomarker protein
TL;DR:
This data set summarized all the putative G4 forming sequences, their biochemical characteristics, and the presence of nuclear localization signal to address the possibility of binding of these PQS region to the target proteins in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 19, 2021
2021 journal article
Detection of Chloride Using Microelectrodes in Closed Bipolar Electrode Scheme
ECS Meeting Abstracts.
Source: ORCID
Added: November 12, 2021
2021 journal article
Development of a POCT type insulin sensor employing anti-insulin single chain variable fragment based on faradaic electrochemical impedance spectroscopy under single frequency measurement
Biosensors and Bioelectronics, 200, 113901.
author keywords: Insulin; Single chain variable fragment (scFv); Faradaic electrochemical impedance; spectroscopy; Point-of-care testing (POCT) sensor; Single frequency measurement
MeSH headings : Biosensing Techniques; Dielectric Spectroscopy; Electrochemical Techniques; Electrodes; Insulin; Point-of-Care Testing; Single-Chain Antibodies
TL;DR:
This study focuses on utilizing the anti-insulin single chain variable fragment (scFv) as a biosensing molecule with single-frequency faradaic electrochemical impedance spectroscopy (EIS) to aid in developing a future point-of-care sensor which rapidly and sensitively measures insulin across a dynamic range of physiological concentrations, with label-free detection.
(via Semantic Scholar)
Source: ORCID
Added: December 21, 2021
2021 journal article
Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement
MOLECULES, 26(3).
author keywords: interdigitated electrode; glycated albumin; fructosyl amino acid oxidase; point of care testing; disposable enzyme sensor; diabetes mellitus
MeSH headings : Biosensing Techniques / instrumentation; Disposable Equipment; Electrodes; Enzymes / metabolism; Glycation End Products, Advanced; Reproducibility of Results; Serum Albumin / analysis; Glycated Serum Albumin
TL;DR:
It is demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 1, 2021
2021 journal article
Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase
BIOSENSORS & BIOELECTRONICS, 177.
author keywords: Haemoglobin A1c; Glycated protein; Fructosyl peptide oxidase; Flow injection analysis; Quasi-direct electron transfer; 2.5th generation principle
MeSH headings : Ascomycota; Biosensing Techniques; Electrons; Flow Injection Analysis; Glycated Hemoglobin / analysis; Humans; Peptides
TL;DR:
Results indicate that the enzyme electrochemical FIA system covers the clinical range of HbA1c detection for more 200 consecutive measurements, and will be possible to develop an integrated system consisting of sample pretreatment and sample electrochemical measurement based on an FIA system possessing quasi-DET type PnFPOx.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 15, 2021
2021 article
Editorial preface of the special issue on "the progress and perspectives of biosensing research in North America"
Li, C.-zhong, Liu, J., & Sode, K. (2021, December 15). BIOSENSORS & BIOELECTRONICS, Vol. 194.
MeSH headings : Biosensing Techniques; North America
Sources: Web Of Science, NC State University Libraries
Added: October 4, 2021
2021 journal article
Electrochemical quantification of accelerated FADGDH rates in aqueous nanodroplets
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 118(25).
author keywords: nanoelectrochemistry; nanodroplet; enzymology; FADGDH; reaction acceleration
MeSH headings : Electrochemistry; Electrodes; Flavin-Adenine Dinucleotide; Glucose Dehydrogenases / metabolism; Nanoparticles / chemistry; Water / chemistry
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: June 16, 2021
2021 journal article
Electrochemical quantification of accelerated FADGDH rates in aqueous nanodroplets
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 118(25).
author keywords: nanoelectrochemistry; nanodroplet; enzymology; FADGDH; reaction acceleration
MeSH headings : Adolescent; Humans; United States / epidemiology; Child; Young Adult; Adult; Suicide, Attempted; Suicidal Ideation; Information Sources; Suicide Prevention; Surveys and Questionnaires; Risk Factors
Sources: Web Of Science, NC State University Libraries
Added: August 2, 2021
2021 journal article
G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol
NUCLEIC ACIDS RESEARCH, 49(11), 6069–6081.
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Computer Simulation; G-Quadruplexes; Heme / metabolism; Luminescence; Luminol / chemistry; Luminol / metabolism; Myoglobin / metabolism; Nuclear Magnetic Resonance, Biomolecular; Peroxidase / metabolism; SELEX Aptamer Technique; Substrate Specificity
TL;DR:
G-quadruplex (G4)-forming DNA aptamers that upregulate the peroxidase activity in myoglobin specifically for luminol are reported, which shows a parallel-type G4 structure with propeller-like loops that might be useful for a rational design of aptasensors utilizing the G4 aptamer-myoglobin pair.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 26, 2021
2021 journal article
Rapid, convenient, and highly sensitive detection of human hemoglobin in serum using a high-affinity bivalent antibody-enzyme complex
TALANTA, 234.
author keywords: Bivalent antibody-enzyme complex; Catcher/tag system; Glucose dehydrogenase; Hemoglobin; Homogeneous electrochemical detection; Single-chain variable fragment
MeSH headings : ErbB Receptors; Hemoglobins; Humans; Single-Chain Antibodies
TL;DR:
A rapid, convenient, and highly sensitive detection method for Hb in human serum using a bivalent antibody-enzyme complex (AEC) and a rapid and wash-free homogeneous electrochemical detection system for HB by integrating magnetic beads are established.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2021
2021 journal article
Transient potentiometry based d-serine sensor using engineered d-amino acid oxidase showing quasi-direct electron transfer property
Biosensors and Bioelectronics, 200, 113927.
author keywords: D-Serine; Transient potentiometry; Open circuit potential; D-Amino acid Oxidase; Direct electron transfer
MeSH headings : Biosensing Techniques; Electrons; Flavin-Adenine Dinucleotide; Glucose; Glucose 1-Dehydrogenase; Potentiometry; Serine
TL;DR:
The transient potentiometry based sensing reported in this study will be further utilized to realize miniaturized, continuous, real-time, in vivo sensor for d-serine monitoring.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: December 30, 2021
2020 journal article
(Keynote) Continuous Glucose Monitoring Systems - Current Status and Future Perspectives of the Flagship Technologies in Biosensor Research -
ECS Meeting Abstracts, MA2020-02(44), 2777–2777.
Source: ORCID
Added: December 23, 2020
2020 journal article
A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria
Marine Biotechnology, 23(1), 31–38.
author keywords: T7 RNA polymerase; Green light-regulated gene expression system; Light-regulated bioprocesses; CcaS; CcaR; Synechocystissp; PCC6803; Cyanobacteria
MeSH headings : Bacterial Proteins; DNA-Directed RNA Polymerases; Gene Expression; Gene Expression Regulation, Bacterial / radiation effects; Genes, Reporter; Green Fluorescent Proteins / genetics; Light; Plasmids; Promoter Regions, Genetic; Synechocystis / genetics; Synechocystis / radiation effects; Viral Proteins
TL;DR:
Comparison with the strong promoter expression systems Pcpc560 and PtrcΔlacO revealed that the expression of sfGFP by the T7 RNAP system was comparable with the levels obtained with strong promoters.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Source: ORCID
Added: October 9, 2020
2020 review
Alteration of Electron Acceptor Preferences in the Oxidative Half-Reaction of Flavin-Dependent Oxidases and Dehydrogenases
[Review of ]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(11).
author keywords: oxidase; dehydrogenase; oxidative half-reaction; oxygen; electron acceptor; flavin adenine dinucleotide; flavin mononucleotide; oxygen accessible pathway; bioelectrochemistry; enzyme engineering
MeSH headings : Animals; Electron Transport; Flavins / chemistry; Flavins / metabolism; Flavoproteins / chemistry; Flavoproteins / metabolism; Humans; Oxidoreductases / chemistry; Oxidoreductases / metabolism; Oxygen / chemistry; Oxygen / metabolism
TL;DR:
In this review, recent progress in the engineering of the oxidative half-reaction of flavin-dependent oxidases and dehydrogenases is discussed, considering their current and future applications in bioelectrochemical studies, such as for the development of biosensors and biofuel cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: July 20, 2020
2020 journal article
Application of a Glucose Dehydrogenase-Fused with Zinc Finger Protein to Label DNA Aptamers for the Electrochemical Detection of VEGF
SENSORS, 20(14).
author keywords: aptamer; labeling; enzyme; zinc finger protein; glucose dehydrogenase; electrochemical sensor; vascular endothelial growth factor
MeSH headings : Aptamers, Nucleotide; Biosensing Techniques; Electrochemical Techniques; Glucose 1-Dehydrogenase; Humans; Vascular Endothelial Growth Factor A / analysis; Zinc Fingers
TL;DR:
This study used GDH that was genetically fused to a DNA binding protein, i.e., zinc finger protein (ZF), to demonstrate the concept of using ZF-fused GDH to enzymatically label aptamers for electrochemical detection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 24, 2020
2020 journal article
Construction of Super-Stabilized Direct Electron Transfer Type Glucose Dehydrogenase for Long Term Continuous Glucose Sensing System
ECS Meeting Abstracts, MA2020-02(44), 2831–2831.
Source: ORCID
Added: December 23, 2020
2020 journal article
Creation of a novel DET type FAD glucose dehydrogenase harboring Escherichia coli derived cytochrome b562 as an electron transfer domain
Biochemical and Biophysical Research Communications, 530(1), 82–86.
author keywords: Fusion enzymes; Direct electron transfer; Enzyme electrode; 3rd generation glucose sensor; Cytochrome b(562); FAD-Dependent glucose dehydrogenase
MeSH headings : Botrytis / genetics; Botrytis / metabolism; Cytochrome b Group / genetics; Cytochrome b Group / metabolism; Electron Transport; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Flavin-Adenine Dinucleotide / metabolism; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Protein Engineering; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Substrate Specificity
TL;DR:
The construction and production of a new DET-type FADGDH is demonstrated using E.coli as the host cells, which is advantageous for future industrial application and further engineering.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2020 journal article
Development of Insulin Sensors Based on Faradaic and Non-Faradaic Electrochemical Impedance Spectroscopy Detection Principle Using Single Chain Antibody (scFv)
ECS Meeting Abstracts, MA2020-02(44), 2783–2783.
Source: ORCID
Added: December 23, 2020
2020 journal article
Development of an Electrochemical Biosensor of Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) for the Assessment of Traumatic Brain Injury (TBI)
ECS Meeting Abstracts, MA2020-02(44), 2784–2784.
Source: ORCID
Added: December 23, 2020
2020 journal article
Electrochemical Aptasensor Employing Enzyme-Aptamer One-to-One Complex Showing Quasi-Direct Electron Transfer Ability
ECS Meeting Abstracts, MA2020-02(44), 2834–2834.
Source: ORCID
Added: December 23, 2020
2020 journal article
Engineered Glucose Oxidase Capable of Quasi-Direct Electron Transfer after a Quick-and-Easy Modification with a Mediator
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(3).
author keywords: glucose oxidase; direct electron transfer; amine-reactive phenazine ethosulfate; glucose sensor; glycemic level monitoring
MeSH headings : Aspergillus niger / enzymology; Biosensing Techniques / methods; Blood Glucose / analysis; Electrochemical Techniques; Fungal Proteins / metabolism; Glucose / metabolism; Glucose Oxidase / genetics; Glucose Oxidase / metabolism; Phenazines / metabolism; Protein Engineering
TL;DR:
E engineered GOx, which was made readily available for single-step modification with a redox mediator on its surface via a lysine residue rationally introduced into the enzyme, showed a quasi-DET response upon the addition of glucose.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 8, 2020
2020 journal article
Rational design of direct electron transfer type L-lactate dehydrogenase for the development of multiplexed biosensor
BIOSENSORS & BIOELECTRONICS, 176.
author keywords: L-lactate oxidase; Direct electron transfer; Fusion protein; Site-directed mutagenesis; Multiplexed biosensor; Biomedical engineering
MeSH headings : Biosensing Techniques; Blood Glucose; Blood Glucose Self-Monitoring; Electrodes; Electrons; Glucose; Glucose Oxidase; L-Lactate Dehydrogenase
TL;DR:
This is the first report of the DET-type enzyme based lactate and glucose dual sensing systems and the impact of the presence of electrochemical interferants was revealed to be negligible.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 8, 2021
2020 journal article
Strategic design and improvement of the internal electron transfer of heme b domain-fused glucose dehydrogenase for use in direct electron transfer-type glucose sensors
BIOSENSORS & BIOELECTRONICS, 176.
author keywords: Glucose dehydrogenase; Surface electrostatics; Direct electron transfer; Fusion protein; Docking simulation
MeSH headings : Biosensing Techniques; Blood Glucose; Blood Glucose Self-Monitoring; Electron Transport; Electrons; Flavin-Adenine Dinucleotide / metabolism; Glucose; Glucose 1-Dehydrogenase / metabolism; Heme; Saccharomycetales
TL;DR:
Results indicated that the sensor employed by the redesigned mutant Pcyb-AfGDH can be used for future continuous glucose monitoring system based on direct electron transfer principle.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 15, 2021
2020 journal article
The Abtamer: A Novel Antibody-Aptamer Conjugate for Electrochemical Biosensing Applications
ECS Meeting Abstracts, MA2020-02(44), 2835–2835.
Source: ORCID
Added: December 23, 2020
2020 journal article
The Continuous 3 Month Operation of Open Circuit Potential Based Glucose Sensor Employing Direct Electron Transfer Type Fad Dependent Glucose Dehydrogenase
ECS Meeting Abstracts, MA2020-02(44), 2779–2779.
Source: ORCID
Added: December 23, 2020
2019 journal article
Application of an engineered chromatic acclimation sensor for red-light-regulated gene expression in cyanobacteria
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 44.
author keywords: Cyanobacteria; Marine cyanobacterium; Chromatic acclimation sensor; CcaS/CcaR two-component system; Miniaturized CcaS; Red-light-regulated gene expression
TL;DR:
The constructed red-light-regulated gene expression system using CcaS#11 has a great potential as a platform technology for the further development of light-regulated bioprocesses with strict control in cyanobacteria.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: December 16, 2019
2019 journal article
FAD dependent glucose dehydrogenases - Discovery and engineering of representative glucose sensing enzymes -
BIOELECTROCHEMISTRY, 132.
author keywords: Flavin adenine dinucleotide (FAD); Glucose dehydrogenase; Glucose sensor; Diabetes; Direct electron transfer (DET); Glucose oxidase
MeSH headings : Electrons; Flavin-Adenine Dinucleotide / metabolism; Fungi / enzymology; Glucose / metabolism; Glucose 1-Dehydrogenase / metabolism; Protein Engineering
TL;DR:
The two representative glucose sensing enzymes are focused on, by presenting their discovery, sources, and protein and enzyme properties, and the current engineering strategies to improve their potential in sensor applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 30, 2020
2019 journal article
G-Quadruplex Structure Improves the Immunostimulatory Effects of CpG Oligonucleotides
NUCLEIC ACID THERAPEUTICS, 29(4), 224–229.
author keywords: CpG ODN; toll-like receptor 9; G-quadruplex; IL-6; peripheral blood mononuclear cells
MeSH headings : Adjuvants, Immunologic / genetics; Adjuvants, Immunologic / pharmacology; Animals; Cell Line; CpG Islands / drug effects; G-Quadruplexes; Humans; Immunologic Factors / genetics; Leukocytes, Mononuclear / drug effects; Leukocytes, Mononuclear / immunology; Nucleic Acid Conformation / drug effects; Oligonucleotides, Antisense / chemistry; Oligonucleotides, Antisense / genetics; Phosphates / chemistry; Phosphates / metabolism
TL;DR:
Results indicate that G-quadruplex formation can be used to increase the immunostimulatory activity of CpG ODNs having a natural PD backbone.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 19, 2019
2019 journal article
Rational engineering of Aerococcus viridans L-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor
BIOSENSORS & BIOELECTRONICS, 151.
author keywords: L-lactate oxidase; Amine reactive phenazine ethosulfate; Site-directed mutagenesis; Amperometric L-lactate biosensor; Interference; Direct electron transfer
MeSH headings : Aerococcus / chemistry; Aerococcus / isolation & purification; Biosensing Techniques; Catalysis; Enzymes, Immobilized / chemistry; Glucose / chemistry; Humans; Lactic Acid / chemistry; Lactic Acid / isolation & purification; Mixed Function Oxygenases / chemistry; Oxidation-Reduction
TL;DR:
Results indicated that a quasi-DET type lactate sensor was developed that did not suffer from the interference of oxygen and representative electroactive ingredient compounds.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 29, 2020
2019 journal article
Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase
BIOSENSORS & BIOELECTRONICS, 129, 189–197.
author keywords: Faradaic electrochemical impedance spectroscopy; Impedimetric biosensor; Direct electron transfer; FAD dependent glucose dehydrogenase complex; Charge transfer resistance; Imaginary impedance monitoring
MeSH headings : Bacteria / enzymology; Biosensing Techniques / methods; Electric Impedance; Electrodes; Electron Transport; Flavin-Adenine Dinucleotide / chemistry; Glucose / analysis; Glucose 1-Dehydrogenase / chemistry; Gold / chemistry
TL;DR:
The 3rd-generation faradaic enzyme EIS sensor would provide alternative platform for future impedimetric immunosensing system, which does not use redox probe, according to its potential application as the future immunosensor platform.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: March 11, 2019
2019 journal article
X-ray structure of the direct electron transfer-type FAD glucose dehydrogenase catalytic subunit complexed with a hitchhiker protein
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 75, 841–851.
author keywords: direct electron transfer; flavin adenine dinucleotide-dependent dehydrogenase complex; glucose dehydrogenase; iron-sulfur cluster; hitchhiker protein; glucose sensors; Burkholderia cepacia
MeSH headings : Burkholderia cepacia / enzymology; Catalytic Domain; Crystallography, X-Ray / methods; Electron Transport; Flavin-Adenine Dinucleotide / chemistry; Glucose Dehydrogenases / chemistry; Models, Molecular; Recombinant Proteins / chemistry
TL;DR:
The X-ray structure of the catalytic subunit of Burkholderia cepacia FAD glucose dehydrogenase complexed with a hitchhiker protein was determined as a representative molecule of direct electron transfer-type FAD-dependent dehydrogenases complexes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 23, 2019
2018 journal article
A Disposable Tear Glucose Biosensor-Part 5: Improvements in Reagents and Tear Sampling Component.
Journal of Diabetes Science and Technology, 12(4), 842–846.
Contributors: C. Lin*, Y. Ito*, A. Deng*, J. Johns*, D. Matloff*, C. Cook *, n , J. La Belle *
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Blood Glucose / analysis; Contact Lenses; Glucose 1-Dehydrogenase; Humans; Indicators and Reagents; Tears / chemistry
TL;DR:
A tear glucose (TG) sensor with an integrated tear sampler can provide a noninvasive method for calibrating the continuous TG contact lens and monitoring glucose and suggests that using enzymes with lower Km may be advantageous when operating in low glucose environments like tears.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2018 journal article
Affinity sensor for haemoglobin A1c based on single-walled carbon nanotube field-effect transistor and fructosyl amino acid binding protein
BIOSENSORS & BIOELECTRONICS, 129, 254–259.
Contributors: M. Hatada*, T. Tran *, W. Tsugawa *, n & A. Mulchandani *
author keywords: Single-walled carbon nanotube; Field-effect transistor; Haemoglobin A1c; Fructosyl amino acid binding protein; SocA; Fructosyl valine
MeSH headings : Agrobacterium tumefaciens / chemistry; Bacterial Proteins / chemistry; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Equipment Design; Glycated Hemoglobin / analysis; Humans; Nanotubes, Carbon / chemistry; Proteolysis; Transistors, Electronic; Valine / analogs & derivatives; Valine / analysis
TL;DR:
The label-free affinity sensor for HbA1c is reported based on the chemiresistor-type field-effect transistor, which has the ability to measure as low as 1.2 nM FV, which is 14-fold more sensitive compared to the previously reported fluorescence-based sensor using SocA.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 20, 2020
2018 journal article
Biomolecular engineering of redox enzymes: Alteration of the electron acceptor preference; from oxygen to direct electron transfer
Abstracts of Papers of the American Chemical Society, 255. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000435537700584&KeyUID=WOS:000435537700584
Source: ORCID
Added: October 9, 2020
2018 journal article
Comprehensive study of domain rearrangements of single-chain bispecific antibodies to determine the best combination of configurations and microbial host cells
Mabs, 10(6), 854–863.
Contributors: R. Asano *, Y. Kuroki*, S. Honma, M. Akabane, S. Watanabe, S. Mayuzumi*, S. Hiyamuta*, I. Kumagai*, n
author keywords: Brevibacillus choshinensis; cancer immunotherapy; CD3; diabody; effective domain order; epidermal growth factor receptor; host cell; Pichia pastoris; small bispecific antibody; tandem scFv
MeSH headings : Antibodies, Bispecific / genetics; Antibodies, Bispecific / immunology; Binding Sites / genetics; Binding Sites / immunology; Brevibacillus / genetics; Cell Line, Tumor; Cell Survival / immunology; Electrophoresis, Polyacrylamide Gel; Escherichia coli / genetics; Genetic Variation; Humans; Pichia / genetics; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / immunology; Recombinant Fusion Proteins / metabolism; Single-Chain Antibodies / genetics; Single-Chain Antibodies / immunology
TL;DR:
This study designed all possible domain orders for scDb and taFv with identical Fv pairs and individually expressed all 16 variants using Escherichia coli, Pichia pastoris, and Brevibacillus choshinensis, finding a promising candidate that exhibited high activity and productivity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: October 9, 2020
2018 journal article
Construction and characterization of flavin adenine dinucleotide glucose dehydrogenase complex harboring a truncated electron transfer subunit
ELECTROCHIMICA ACTA, 277, 276–286.
Contributors: J. Okuda-Shimazaki *, N. Loew n, N. Hirose n, K. Kojima *, K. Mori*, W. Tsugawa *, n
author keywords: Direct electron transfer; Truncated electron transfer subunit; Heme c; Glucose dehydrogenase; Glucose sensing
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Designer fungus FAD glucose dehydrogenase capable of direct electron transfer
BIOSENSORS & BIOELECTRONICS, 123, 114–123.
Contributors: K. Ito *, J. Okuda-Shimazaki *, K. Mori*, K. Kojima *, W. Tsugawa *, K. Ikebukuro *, C. Lin *, J. La Belle *, H. Yoshida *, n
author keywords: Glucose dehydrogenase; Cellobiose dehydrogenase; Direct electron transfer; Designer FADGDH; Fusion protein; Heme b-binding cytochrome domain
MeSH headings : Aspergillus flavus / chemistry; Aspergillus flavus / enzymology; Biosensing Techniques; Blood Glucose / isolation & purification; Catalytic Domain; Electrodes; Electron Transport; Flavin-Adenine Dinucleotide / chemistry; Glucose Dehydrogenases / chemistry; Heme / chemistry
TL;DR:
The designer FADGDH was confirmed to be a novel GDH that possesses electrode DET ability and relevant and consistent findings provide a novel strategic approach for the improvement of the DET properties of designer FadGDH.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 20, 2020
2018 journal article
Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference
BIOELECTROCHEMISTRY, 121, 185–190.
Contributors: M. Hatada*, N. Loew n, Y. Inose-Takahashi*, J. Okuda-Shimazaki *, W. Tsugawa *, A. Mulchandani *, n
author keywords: 2.5th generation biosensor; Electrochemical enzyme sensor; Mediator modification of enzymes; Glucose dehydrogenase; Glucoside 3-dehydrogenase; Lactate oxidase
MeSH headings : Aerococcus / enzymology; Agrobacterium tumefaciens / enzymology; Biosensing Techniques / methods; Blood Glucose / analysis; Botrytis / enzymology; Electron Transport; Enzymes, Immobilized / chemistry; Glucose / analysis; Glucose 1-Dehydrogenase / chemistry; Glucose Dehydrogenases / chemistry; Humans; Mixed Function Oxygenases / chemistry; Phenazines / chemistry; Recombinant Proteins / chemistry
TL;DR:
Various kinds of diagnostic enzymes can achieve quasi-DET ability simply by modification with arPES, suggesting that continuous monitoring systems based on the 2.5th generation principle can be developed for various target molecules.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Development of a third-generation glucose sensor based on the open circuit potential for continuous glucose monitoring
BIOSENSORS & BIOELECTRONICS, 124, 216–223.
author keywords: Open circuit potential; Third generation glucose sensor; Continuous glucose monitoring (CGM); Direct electron transfer; FAD dependent glucose dehydrogenase (FADGDH)
MeSH headings : Biosensing Techniques; Blood Glucose / chemistry; Blood Glucose Self-Monitoring / methods; Diabetes Mellitus / blood; Diabetes Mellitus / pathology; Flavin-Adenine Dinucleotide / chemistry; Glucose / chemistry; Glucose / isolation & purification; Glucose 1-Dehydrogenase / chemistry; Humans; Insulin / chemistry; Insulin / metabolism
TL;DR:
The development of the third-generation type open circuit potential (OCP) principle-based glucose sensor with direct electron transfer FADGDH immobilized on gold electrodes using a self-assembled monolayer (SAM) is reported, and a novel algorithm for OCP- based glucose sensors is developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 31, 2018
2018 journal article
Development of an electrochemical detection system for CpG methylation of human genome using methyl CpG binding domain and zinc finger protein
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600001002&KeyUID=WOS:000447600001002
Contributors: J. Lee, W. Yoshida, D. Hiraoka, A. Tatsumi, K. Abe, K. Nakabayashi, H. Wakeda, K. Hata
Source: ORCID
Added: October 9, 2020
2018 journal article
Development toward a novel integrated tear lactate sensor using Schirmer test strip and engineered lactate oxidase
SENSORS AND ACTUATORS B-CHEMICAL, 270, 525–529.
Contributors: C. Lin *, K. Hiraka *, D. Matloff*, J. Johns*, A. Deng*, n , J. La Belle *
author keywords: Tear lactate; Schirmer's test strip; Integrated sensor; Lactate oxidase; Oxygen interference
TL;DR:
The proposed tear lactate (TL) sensor exhibited potential usefulness in providing an alternative noninvasive method of measuring lactate and in calibrating the continuous lactate contact lens.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Direct electron transfer (DET) mechanism of FAD dependent dehydrogenase complexes ∼from the elucidation of intra- and inter-molecular electron transfer pathway to the construction of engineered DET enzyme complexes∼
Current Opinion in Electrochemistry.
Contributors: Y. Yamashita *, I. Lee *, N. Loew n & n
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: October 9, 2020
2018 article
Elucidation of the intra- and inter-molecular electron transfer pathways of glucoside 3-dehydrogenase
Miyazaki, R., Yamazaki, T., Yoshimatsu, K., Kojima, K., Asano, R., Sode, K., & Tsugawa, W. (2018, August). BIOELECTROCHEMISTRY, Vol. 122, pp. 115–122.
Contributors: R. Miyazaki*, T. Yamazaki *, K. Yoshimatsu *, K. Kojima *, R. Asano *, n , W. Tsugawa *
author keywords: Glucoside 3 dehydrogenase; Electron transfer pathway; FAD; Fe-S cluster; Heme c
MeSH headings : Agrobacterium tumefaciens / chemistry; Agrobacterium tumefaciens / enzymology; Agrobacterium tumefaciens / genetics; Amino Acid Sequence; Catalytic Domain; Electron Transport; Genes, Bacterial; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Glucose Dehydrogenases / metabolism; Iron-Sulfur Proteins / chemistry; Iron-Sulfur Proteins / genetics; Iron-Sulfur Proteins / metabolism; Multigene Family; Sequence Alignment
TL;DR:
The results suggested that R. radiobacter G3DH possesses an iron‑sulfur cluster that may play an important role in the electron transfer from FAD to cytochrome c like molecule, which is an external electron acceptor of G3 DH.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Engineered fungus derived FAD-dependent glucose dehydrogenase with acquired ability to utilize hexaammineruthenium(III) as an electron acceptor
Bioelectrochemistry, 123, 62–69.
Contributors: M. Okurita*, N. Suzuki*, N. Loew n, H. Yoshida *, W. Tsugawa *, K. Mori*, K. Kojima *, D. Klonoff *, n
author keywords: Glucose dehydrogenase; Glucose oxidase; Flavin adenine dinucleotide; Aspergillus flavus; Hexaammineruthenium chloride; Enzyme sensor strip
MeSH headings : Amino Acid Substitution; Aspergillus flavus / enzymology; Aspergillus flavus / genetics; Aspergillus flavus / metabolism; Electrochemical Techniques; Electrons; Flavin-Adenine Dinucleotide / metabolism; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Models, Molecular; Protein Engineering; Ruthenium Compounds / metabolism
TL;DR:
A mutant of Aspergillus flavus-derived FADGDH, capable of utilizing hexaammineruthenium(III) as electron acceptor: AfGDH-H403D, which should lead to the development of sensor strips for blood glucose monitoring with increased accuracy and less stringent packing requirements.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: October 9, 2020
2018 journal article
Esterification of PQQ Enhances Blood-Brain Barrier Permeability and Inhibitory Activity against Amyloidogenic Protein Fibril Formation.
ACS Chemical Neuroscience.
Contributors: K. Tsukakoshi *, W. Yoshida *, M. Kobayashi *, N. Kobayashi*, J. Kim *, T. Kaku*, T. Iguchi*, K. Nagasawa *
author keywords: Pyrroloquinoline quinone trimethylester; alpha-synuclein; amyloid beta; blood-brain barrier permeability
MeSH headings : Amyloid / drug effects; Amyloid beta-Peptides / drug effects; Amyloid beta-Peptides / metabolism; Amyloidogenic Proteins / drug effects; Animals; Blood-Brain Barrier / metabolism; Cell Line, Tumor; Cell Survival / drug effects; Esterification; Esters / chemical synthesis; Esters / pharmacology; Humans; Mice; Neurons / drug effects; Neurons / metabolism; PQQ Cofactor / analogs & derivatives; PQQ Cofactor / chemical synthesis; PQQ Cofactor / pharmacology; Peptide Fragments / drug effects; Peptide Fragments / metabolism; Permeability; Prion Proteins / drug effects; Prion Proteins / metabolism; Protein Aggregation, Pathological / metabolism; alpha-Synuclein / drug effects; alpha-Synuclein / metabolism
TL;DR:
It is shown that an esterified compound of PQQ, P QQ-trimethylester (PQQ-TME), has twice the BBB permeability than PqQ in vitro, and indicated that esterification of PZQ could be a useful approach in developing a novel PQZ-based amyloid inhibitor.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2018 journal article
Glycogen Production in Marine Cyanobacterial Strain Synechococcus sp NKBG 15041c
Marine Biotechnology, 20(2), 109–117.
Contributors: A. Badary *, S. Takamatsu*, M. Nakajima*, S. Ferri *, P. Lindblad * & *
author keywords: Marine cyanobacteria; Synechococcus; Glycogen; Bioprocess; Carbohydrate production
MeSH headings : Biofuels; Glycogen / biosynthesis; Nitrogen / metabolism; Seawater; Synechococcus / genetics; Synechococcus / growth & development; Synechococcus / metabolism; Transcription, Genetic; Transcriptome
TL;DR:
The transcriptional analyses revealed that glycogen production in NKBG15041c under nitrogen-depleted conditions can be explained by the contribution of both increased carbon flux towards glycogen synthesis, similar to PCC6803 and PCC7002, and increased transcriptional levels of genes responsible for glycogens synthesis, resulting in a relatively high amount of glycogen under ambient conditions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2018 journal article
Improving the induction fold of riboregulators for cyanobacteria
Rna Biology, 15(3), 353–358.
Contributors: I. Sakamoto*, K. Abe *, S. Kawai*, K. Tsukakoshi *, Y. Sakai*, * , K. Ikebukuro *
author keywords: Cyanobacteria; post-transcriptional gene; regulation; riboregulator; RNA engineering; synthetic biology
MeSH headings : Gene Expression Regulation, Bacterial; Genetic Engineering / methods; Models, Molecular; Nucleic Acid Conformation; Promoter Regions, Genetic; RNA / chemistry; RNA / genetics; Synechocystis / genetics; Synechocystis / growth & development; Transcriptional Activation
TL;DR:
An improved riboregulator is improved by designing two RNA species, taRNA and crRNA, to enhance its induction fold and inserting nucleotides into the crRNA loop to enhance intermolecular hybridization and successfully improved its induction Fold.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2018 journal article
Mutagenesis Study of the Cytochrome c Subunit Responsible for the Direct Electron Transfer-Type Catalytic Activity of FAD-Dependent Glucose Dehydrogenase
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19(4).
Contributors: Y. Yamashita *, N. Suzuki*, N. Hirose*, K. Kojima *, W. Tsugawa * & n
author keywords: glucose dehydrogenase; direct electron transfer; cytochrome c; glucose sensor; biomedical engineering
MeSH headings : Amino Acid Sequence; Biocatalysis; Catalytic Domain / genetics; Cytochromes c / chemistry; Cytochromes c / genetics; Electrodes; Electrons; Enzyme Assays; Flavin-Adenine Dinucleotide / metabolism; Glucose 1-Dehydrogenase / metabolism; Mutagenesis / genetics; Mutant Proteins / metabolism; Solutions
TL;DR:
A mutagenesis investigation of the heme axial ligands was carried out to elucidate the electron transfer pathway to the electron mediators and/or the electrode, and two electron transfer pathways were proposed depending on the electron acceptors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2018 journal article
Quasi-direct electron transfer-type glucose sensor based on engineered glucose oxidase
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600001209&KeyUID=WOS:000447600001209
Source: ORCID
Added: October 9, 2020
2018 journal article
Self-powered, wireless continuous glucose sensing system based direct electron transfer
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600000887&KeyUID=WOS:000447600000887
Source: ORCID
Added: October 9, 2020
2018 journal article
Smart aptamers forming G-quadruplex: Structural and functional change of aptamers forming G-quadruplex in response to surrounding conditions and its regulation with the ligands
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600001223&KeyUID=WOS:000447600001223
Source: ORCID
Added: October 9, 2020
2018 journal article
Synthesis of a hemin-containing copolymer as a novel immunostimulator that induces IFN-gamma production
International Journal of Nanomedicine, 13, 4461–4472.
Contributors: K. Hoshi*, T. Yamazaki *, C. Yoshikawa *, W. Tsugawa *, K. Ikebukuro * & *
author keywords: hemin copolymer; immunostimulator; adjuvant; radical polymerization; NIPAM; toll-like receptor 9; T-helper 1; inflammasome
MeSH headings : Acrylic Resins / chemical synthesis; Acrylic Resins / chemistry; Cell Death / drug effects; Cell Line; Hemin / pharmacology; Humans; Immunologic Factors / pharmacology; Interferon-gamma / biosynthesis; Interleukin-6 / biosynthesis; NF-kappa B / metabolism; Polymers / chemical synthesis; Polymers / chemistry; Signal Transduction / drug effects; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Toll-Like Receptors / metabolism
TL;DR:
It is found that NIPAM-hemin induced IL-6 and IFN-γ production in immune cells without any cytotoxic effects, and has potential therapeutic applications as a Th1-type adjuvant.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: October 9, 2020
2018 journal article
Wearable and implantable biosensing technologies based on the direct electron transfer principle
Abstracts of Papers of the American Chemical Society, 256. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000447600001124&KeyUID=WOS:000447600001124
Source: ORCID
Added: October 9, 2020
2017 journal article
Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp PCC 6803
Applied Microbiology and Biotechnology, 101(23-24), 8465–8474.
Contributors: K. Ueno *, Y. Sakai*, C. Shono*, I. Sakamoto*, K. Tsukakoshi *, Y. Hihara *, * , K. Ikebukuro *
author keywords: Riboregulator; Synechocystis sp PCC 6803; Cyanobacteria; cyabrB2; Gene regulation; Glycogen production
MeSH headings : Gene Expression Regulation, Bacterial; Genes, Regulator; Glycogen / biosynthesis; Metabolic Engineering / methods; Synechocystis / genetics; Synechocystis / metabolism; Transcription, Genetic
TL;DR:
Phenotypic similarities between the cyabrB2 expression-repressed mutant and the ΔcyabRB2 mutant suggest that the riboregulator can potentially be used as a new chromosomal gene regulation tool in cyanobacteria.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
Characterization of Electron Mediator Preference of Aerococcus viridans-Derived Lactate Oxidase for Use in Disposable Enzyme Sensor Strips
Sensors and Materials, 29(12), 1703–1711.
Contributors: N. Loew*, M. Fitriana *, K. Hiraka *, * & W. Tsugawa *
author keywords: lactate oxidase; screen-printed carbon electrode; electron mediator; 1-methoxy-5-methyl phenazinium methyl sulfate; potassium ferricyanide; hexaammineruthenium(III) chloride; biomedical engineering
TL;DR:
The protein scaffold of Av LOx can almost completely block the access to the co-factor for very small electron acceptors, depending on their charge.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
Mediator Preference of Two Different FAD-Dependent Glucose Dehydrogenases Employed in Disposable Enzyme Glucose Sensors
Sensors, 17(11).
Contributors: N. Loew*, W. Tsugawa *, D. Nagae*, K. Kojima * & *
author keywords: glucose dehydrogenase; flavin adenine dinucleotide; Aspergillus flavus; Burkholderia cepacia; enzyme sensor strip; mediated electron transfer; potassium ferricyanide; methoxy phenazine methosulfate; hexaammineruthenium chloride
MeSH headings : Biosensing Techniques; Flavin-Adenine Dinucleotide; Glucose / analysis; Glucose Dehydrogenases
TL;DR:
Results show that access of even very small mediators with strong charges to a buried active center can be almost completely blocked by the protein.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 chapter
Minimally invasive microneedle array electrodes employing direct electron transfer type glucose dehydrogenase for the development of continuous glucose monitoring sensors
In Biosensors 2016 (Vol. 27, pp. 208–209).
Contributors: S. Sharma *, E. Takagi*, T. Cass *, W. Tsugawa * & *
author keywords: continuous glucose monitoring; minimally invasive sensors; direct electron transfer; microneedles
TL;DR:
This work reports on the marriage of minimally invasive, continuous glucose sensors and a direct electron transfer type glucose dehydrogenase enzymatic system, which allows operation of the electrochemical sensor at lower potentials to minimize the effect of interference.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
Minimizing the effects of oxygen interference on L-lactate sensors by a single amino acid mutation in Aerococcus viridans L-lactate oxidase
Biosensors & Bioelectronics, 103, 163–170.
Contributors: K. Hiraka *, K. Kojima *, C. Lin *, W. Tsugawa *, R. Asano *, J. La Belle *, *
author keywords: L-lactate oxidase; L-lactate; Oxygen; Screen-printed carbon electrode; Site-directed mutagenesis; Biomedical engineering
MeSH headings : Amino Acids / chemistry; Biosensing Techniques; Lactic Acid / chemistry; Lactic Acid / isolation & purification; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / genetics; Mutation; Oxidation-Reduction; Oxygen / chemistry
TL;DR:
This study provided significant insight into the enzymatic reaction mechanism of AvLOx and presented a novel approach to minimize oxygen interference in sensor applications, which will enable accurate detection of l-lactate concentrations.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2017 journal article
The electrochemical behavior of a FAD dependent glucose dehydrogenase with direct electron transfer subunit by immobilization on self-assembled monolayers
Bioelectrochemistry, 121, 1–6.
Contributors: I. Lee *, N. Loew*, W. Tsugawa *, C. Lin *, D. Probst *, J. La Belle *, n
MeSH headings : Biosensing Techniques / methods; Blood Glucose / analysis; Blood Glucose / metabolism; Burkholderia cepacia / enzymology; Electrochemical Techniques / methods; Electron Transport; Enzymes, Immobilized / metabolism; Flavin-Adenine Dinucleotide / metabolism; Glucose / analysis; Glucose / metabolism; Glucose 1-Dehydrogenase / metabolism; Humans
TL;DR:
The results indicate the feasibility of controlling the immobilization state of the enzymes on the electrode surface, and the current generated from DET-FADGDH electrodes decreased with increasing length of SAM, suggesting that the DET ability can be affected by the distance between the enzyme and the electrode.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018
2017 journal article
X-ray structures of fructosyl peptide oxidases revealing residues responsible for gating oxygen access in the oxidative half reaction
Scientific Reports, 7(1).
Contributors: T. Shimasaki *, H. Yoshida *, S. Kamitori * & *
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Substitution; Amino Acids / chemistry; Amino Acids / genetics; Amino Acids / metabolism; Binding Sites; Crystallography, X-Ray; Flavin-Adenine Dinucleotide / chemistry; Flavin-Adenine Dinucleotide / metabolism; Glycated Hemoglobin / metabolism; Models, Molecular; Mutagenesis, Site-Directed; Oxidation-Reduction; Oxygen / chemistry; Oxygen / metabolism; Protein Conformation; Structure-Activity Relationship
TL;DR:
Results reveal that Asn56 in PnFPOX is essential for maintaining an effective oxygen accession path, and support the role of Asp54 as a gate keeper that cooperates with Lys274 to enable oxygen to reach the active site properly.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2016 journal article
An Fe-S cluster in the conserved Cys-rich region in the catalytic subunit of FAD-dependent dehydrogenase complexes
Bioelectrochemistry, 112, 178–183.
Contributors: M. Shiota*, T. Yamazaki *, K. Yoshimatsu *, K. Kojima *, W. Tsugawa *, S. Ferri *, *
author keywords: Fe-S cluster; Glucose dehydrogenase; FAD; Direct electron transfer; FAD-dependent dehydrogenase
MeSH headings : Amino Acid Sequence; Catalytic Domain; Conserved Sequence; Cysteine; Flavin-Adenine Dinucleotide / metabolism; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Iron; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Sulfur
TL;DR:
Three Cys residues in the Cys-rich region constitute an iron-sulfur cluster that may play an important role in the electron transfer from FAD (intra-molecular) to the multi-heme cytochrome c subunit (inter-molescular) electron transfer pathway.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal
Scientific Reports, 6.
Contributors: M. Nakajima*, S. Ferri, M. Rogner * & *
MeSH headings : Acclimatization; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Cells, Cultured; Cyanobacteria / metabolism; Fluorescence; Gene Expression Regulation, Bacterial; Light Signal Transduction; Miniaturization / instrumentation; Models, Molecular; Protein Domains
TL;DR:
This study attempted to engineer the chromatic acclimation sensor CcaS, a CBCR derived from the cyanobacterium Synechocystis sp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Continuous operation of an ultra-low-power microcontroller using glucose as the sole energy source
Biosensors & Bioelectronics, 93, 335–339.
Contributors: I. Lee *, T. Sode*, N. Loew*, W. Tsugawa *, C. Lowe * & *
author keywords: Microcontroller; BioCapacitor; Enzyme fuel cell; Direct electron transfer; Implantable artificial organs; Self-powered; Glucose sensor
MeSH headings : Artificial Organs; Bioelectric Energy Sources; Biosensing Techniques; Electrodes; Glucose / chemistry; Glucose 1-Dehydrogenase / chemistry; Humans
TL;DR:
The success in operating a microcontroller using glucose as the sole energy source indicated the probability of realizing implantable self-powered autonomously operated artificial organs, such as artificial pancreas.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2016 journal article
Development of a light-regulated cell-recovery system for non-photosynthetic bacteria
Microbial Cell Factories, 15(1).
Contributors: M. Nakajima*, K. Abe *, S. Ferri * & *
author keywords: Antigen 43; Green light induction; Cell recovery; Green light sensor; Optogenetics; Escherichia coli
MeSH headings : Escherichia coli / cytology; Escherichia coli / radiation effects; Genetic Vectors / metabolism; Light; Photosynthesis / radiation effects; Plasmids / metabolism; Synechocystis / radiation effects; Time Factors; Transcription, Genetic / radiation effects
TL;DR:
This study demonstrated the construction of a cell recovery system for non-photosynthetic microorganisms induced by exposure of cells to green light and represents a novel tool for future bioprocessing with reduced energy and labor required for cell recovery.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Development of a screen-printed carbon electrode based disposable enzyme sensor strip for the measurement of glycated albumin
Biosensors & Bioelectronics, 88, 167–173.
Contributors: M. Hatada*, W. Tsugawa *, E. Kamio*, N. Loew*, D. Klonoff * & *
author keywords: Diabetes mellitus; Glycated albumin; Fructosyl amino acid oxidase; Hemoglobin A1c; Point-of-care testing; Screen-printed carbon electrode
MeSH headings : Amino Acid Oxidoreductases / chemistry; Biosensing Techniques / instrumentation; Carbon / chemistry; Electrodes; Equipment Design; Glycation End Products, Advanced; Humans; Limit of Detection; Point-of-Care Systems; Reagent Strips / analysis; Serum Albumin / analysis; Glycated Serum Albumin
TL;DR:
The development of a sensing system for measuring GA by combining an enzyme analysis method, which is already used in clinical practice, with electrochemical principles is reported, which shows a clear correlation between the GA concentration and the resulting current.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Development of an electrochemical detection system for measuring DNA methylation levels using methyl CpG-binding protein and glucose dehydrogenase-fused zinc finger protein
Biosensors & Bioelectronics, 93, 118–123.
Contributors: J. Lee *, W. Yoshida *, K. Abe *, K. Nakabayashi *, H. Wakeda*, K. Hata *, C. Marquette *, L. Blum*, * , K. Ikebukuro *
author keywords: DNA methylation; Electrochemical detection; Diagnosis; Zinc finger protein; Glucose dehydrogenase; Methyl-CpG binding domain
MeSH headings : Biosensing Techniques; CpG Islands / genetics; DNA / chemistry; DNA Methylation / genetics; DNA-Binding Proteins / chemistry; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / genetics; Humans; Promoter Regions, Genetic
TL;DR:
A simple electrochemical detection system for DNA methylation using methyl CpG-binding domain (MBD) and a glucose dehydrogenase (GDH)-fused zinc finger protein is developed and the methylation levels at the promoter region of the androgen receptor gene are measured.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Electrochemical sensing system employing fructosamine 6-kinase enables glycated albumin measurement requiring no proteolytic digestion
Biotechnology Journal, 11(6), 797–804.
Contributors: M. Kameya*, W. Tsugawa *, M. Yamada-Tajima*, M. Hatada*, K. Suzuki *, A. Sakaguchi-Mikami*, S. Ferri *, D. Klonoff *, *
author keywords: Diabetes; Fructosamine 6-kinase; Glycated albumin; Glycated protein; Proteolytic digestion
MeSH headings : Biosensing Techniques / methods; Diabetes Mellitus / blood; Electrochemical Techniques / methods; Escherichia coli / enzymology; Escherichia coli Proteins / chemistry; Fructosamine / chemistry; Glycation End Products, Advanced; Humans; Oxidoreductases / chemistry; Phosphotransferases (Alcohol Group Acceptor) / chemistry; Proteolysis; Pyruvate Kinase / chemistry; Serum Albumin / analysis; Glycated Serum Albumin
TL;DR:
An electrochemical sensor based on fructosamine 6‐kinase (FN6K) from Escherichia coli, which can react directly with intact GA unlike FAOXs/FPOXs and could be applied to point‐of‐care‐testing devices for measurement of glycated proteins.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Novel fungal FAD glucose dehydrogenase derived from Aspergillus niger for glucose enzyme sensor strips
Biosensors & Bioelectronics, 87, 305–311.
Contributors: * , N. Loew*, Y. Ohnishi*, H. Tsuruta*, K. Mori*, K. Kojima *, W. Tsugawa *, J. LaBelle *, D. Klonoff *
author keywords: FAD-dependent glucose dehydrogenase; Aspergillus niger; Aspergillus flavus; Glucose specificity; Xylose; BG Monitor
MeSH headings : Aspergillus niger / chemistry; Aspergillus niger / enzymology; Aspergillus niger / metabolism; Biosensing Techniques / methods; Flavin-Adenine Dinucleotide / metabolism; Glucose / analysis; Glucose / metabolism; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / metabolism; Humans; Models, Molecular; Substrate Specificity; Xylose / metabolism
TL;DR:
This is the first report of an enzyme sensor strip using a fungus derived FADGDH, which did not show any positive bias at a therapeutic level xylose concentration on the signal for a glucose sample, which clearly indicates the superiority of AnGDH over other conventionally used fungi derived F ADGDHs in the application for SMBG sensor strips.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2016 journal article
Structural regulation by a G-quadruplex ligand increases binding abilities of G-quadruplex-forming aptamers
Chemical Communications, 52(85), 12646–12649.
Contributors: K. Tsukakoshi *, Y. Ikuta*, K. Abe *, W. Yoshida *, K. Iida *, Y. Ma *, K. Nagasawa *, * , K. Ikebukuro *
TL;DR:
It is reported that a G-quadruplex-specific compound induced structural changes in G- Quadruple-forming DNA aptamers and stabilized the folded structures, making them suitable for targeted proteins and enhancing their binding properties.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
3RD GENERATION CGM SENSOR EMPLOYING DIRECT ELECTRON TRANSFER PRINCIPLE WITH IMPROVED OPERATIONAL CONDITIONS
Diabetes Technology & Therapeutics, 17, A90. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000349317500203&KeyUID=WOS:000349317500203
Source: ORCID
Added: October 9, 2020
2015 journal article
Advancing the development of glycated protein biosensing technology: next-generation sensing molecules.
Journal of Diabetes Science and Technology, 9(2), 183–191.
Contributors: M. Kameya*, A. Sakaguchi-Mikami*, S. Ferri *, W. Tsugawa * & *
MeSH headings : Amino Acid Oxidoreductases; Biomarkers / analysis; Biosensing Techniques / methods; Fructosamine; Humans
TL;DR:
Research on FN6K and fructosyl amino acid-binding protein has been opening up new possibilities for the development of highly sensitive and proteolytic-digestion-free biosensing systems for glycated proteins.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
BioCapacitor: A novel principle for biosensors
Biosensors & Bioelectronics, 76, 20–28.
Contributors: * , T. Yamazaki *, I. Lee *, T. Hanashi* & W. Tsugawa *
author keywords: BioCapacitor; Enzyme fuel cell; Charge pump; Implantable devices; Continuous glucose monitoring
MeSH headings : Biocatalysis; Bioelectric Energy Sources; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Electricity; Enzymes / chemistry; Glucose / isolation & purification; Glucose / metabolism; Humans
TL;DR:
The BioCapacitor principle, a novel biodevice based on an enzyme fuel cell that generates sufficient stable power to operate electric devices, is described and the recent challenges in the developments of self-powered stand-alone biodevices employing enzyme fuel cells combined with charge pumps and capacitor are introduced.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 article
Crystal structure of Aspergillus flavus FAD glucose dehydrogenase
Worldwide Protein Data Bank.
Source: ORCID
Added: October 9, 2020
2015 article
Crystal structure of Aspergillus flavus FADGDH in complex with D-glucono-1,5-lactone
Worldwide Protein Data Bank.
Source: ORCID
Added: October 9, 2020
2015 journal article
Development of an electrochemical adenosine diphosphate (ADP) sensing principle employing ADP-dependent kinase
Sensors and Materials, 27(9), 839–845.
Source: ORCID
Added: October 9, 2020
2015 journal article
Efficient surface-display of autotransporter proteins in cyanobacteria
Algal Research-Biomass Biofuels and Bioproducts, 12, 337–340.
Contributors: S. Ferri *, M. Nakamura*, A. Ito *, M. Nakajima*, K. Abe *, K. Kojima *, *
author keywords: Cyanobacteria; Synechocystis; Autotransporter; Cell-surface display; Recombinant expression
TL;DR:
This is the first report of an efficient method for the display of recombinant proteins in cyanobacteria and the complete post-translational processing of the antigen 43 protein was confirmed by Western blot analysis.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Enzyme linking to DNA aptamers via a zinc finger as a bridge
Chemical Communications, 51(57), 11467–11469.
Contributors: K. Abe *, Y. Murakami *, A. Tatsumi*, K. Sumida*, A. Kezuka*, T. Fukaya*, T. Kumagai*, Y. Osawa*, * , K. Ikebukuro *
MeSH headings : Animals; Aptamers, Nucleotide / chemistry; Biosensing Techniques; Cattle; Enzyme-Linked Immunosorbent Assay; Humans; Luciferases, Firefly / chemistry; Luminescent Agents / chemistry; Luminescent Measurements; Thrombin / analysis; Vascular Endothelial Growth Factor A / analysis; Zinc Fingers
TL;DR:
A novel enzyme-labeling method for DNA aptamers using enzyme-fused zinc finger proteins that achieved thrombin detection and vascular endothelial growth factor detection using zinc finger-fuse firefly luciferase is proposed.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in Enterobacter aerogenes
Microbial Cell Factories, 14(1), (11 June 2015).
Contributors: Y. Tajima*, Y. Yamamoto *, K. Fukui *, Y. Nishio*, K. Hashiguchi*, Y. Usuda *, *
author keywords: Enterobacter aerogenes; Succinate fermentation; Phosphoenolpyruvate carboxykinase; Glucose-PTS; Weak acidity; Anaerobic metabolism
MeSH headings : Adenosine Triphosphate / metabolism; Anaerobiosis; Culture Media / chemistry; Culture Media / metabolism; Enterobacter aerogenes / genetics; Enterobacter aerogenes / metabolism; Fermentation; Hydrogen-Ion Concentration; Metabolic Engineering / methods; Succinic Acid / metabolism
TL;DR:
Focusing on increasing net ATP production during succinate synthesis leads to increased succinate yield and volumetric productivity in E. aerogenes, demonstrating proof-of-concept that bacterium-based succinate fermentation can be improved under weakly acidic conditions.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Improvement of the VEGF binding ability of DNA aptamers through in silico maturation and multimerization strategy
Journal of Biotechnology, 212, 99–105.
Contributors: T. Fukaya*, K. Abe *, N. Savory *, K. Tsukakoshi *, W. Yoshida *, S. Ferri *, * , K. Ikebukuro *
author keywords: Aptamer; in silico maturation; Multimerization; Vascular endothelial growth factor
MeSH headings : Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / metabolism; Computer Simulation; Protein Binding; Protein Multimerization; Surface Plasmon Resonance; Vascular Endothelial Growth Factor A / genetics; Vascular Endothelial Growth Factor A / metabolism
TL;DR:
Improved DNA aptamer binding affinity for vascular endothelial growth factor (VEGF) through in silico maturation (ISM) and aptamer multimerization and designed SL5-trivalent aptamer with three binding motifs significantly increased binding affinity, representing a 500-fold improvement.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Klaus Mosbach Tribute
Biotechnology and Bioengineering, 112(4), 645–647.
Contributors: D. Clark *, I. Karube*, * , L. Bulow *, O. Brueggemann, C. Lowe *, P. Poulsen, R. Langer*
MeSH headings : Bioengineering / history; Bioengineering / methods; Biomedical Research / trends; Biosensing Techniques / methods; Biotechnology / history; Biotechnology / methods; History, 20th Century; History, 21st Century; Molecular Imprinting / methods
TL;DR:
Arevelatory theme of Klaus Mosbach’s work–thatbiomolecules and biopolymers could be controllably modified,manipulated, and assembled together in new ways, almost likeinterchangeablepartsofsomegrandmachine–was incrediblyexciting, andcontinued to driveriveinnovationsacrossmanydifferentdisciplines.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Scaffold-fused riboregulators for enhanced gene activation in Synechocystis sp PCC 6803
Microbiologyopen, 4(4), 533–540.
Contributors: Y. Sakai*, K. Abe *, S. Nakashima *, J. Ellinger *, S. Ferri *, * , K. Ikebukuro *
author keywords: Hfq; posttranscriptional gene regulation; riboregulator; Synechocystis sp PCC 6803; synthetic biology
MeSH headings : Gene Expression Regulation, Bacterial; Gene Targeting / methods; Molecular Biology / methods; Synechocystis / genetics; Synthetic Biology / methods; Transcriptional Activation
TL;DR:
The scaffold sequence derived from natural E. coli noncoding small RNAs is effective for designing RNA‐based genetic tools and scaffold‐fused riboregulators are a strong RNA‐tool to regulate gene expression in cyanobacteria.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Stabilization of fungi-derived recombinant FAD-dependent glucose dehydrogenase by introducing a disulfide bond
Biotechnology Letters, 37(5), 1091–1099.
Contributors: G. Sakai*, K. Kojima *, K. Mori*, Y. Oonishi* & *
author keywords: Aspergillus flavus; Disulfide bond; FAD; Glucose dehydrogenase; Site directed mutagenesis; Thermal stability
MeSH headings : Disulfides / chemistry; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Kinetics; Mutagenesis, Site-Directed; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; Temperature
TL;DR:
The FAD-glucose dehydrogenase (GDH) mutant Val149Cys/Gly190Cys, which was constructed based on a comparison with the three dimensional structure of glucose oxidase showed a 110 min half-life of thermal inactivation at 45 °C, which is 13-fold greater than that of the wild-type enzyme.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
Structural analysis of fungus-derived FAD glucose dehydrogenase
Scientific Reports, 5.
Contributors: H. Yoshida *, G. Sakai*, K. Mori*, K. Kojima *, S. Kamitori * & *
MeSH headings : Aspergillus / enzymology; Catalytic Domain; Crystallography, X-Ray; Flavin-Adenine Dinucleotide / metabolism; Gluconates / metabolism; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / metabolism; Histidine / chemistry; Hydrogen Bonding; Lactones / metabolism; Models, Molecular; Mutagenesis, Site-Directed; Oxygen / metabolism; Substrate Specificity
TL;DR:
The first three-dimensional structure of fungus-derived glucose dehydrogenase using flavin adenine dinucleotide (FAD) as the cofactor is reported, similar to that of fungal glucose oxidases (GOxs) reported till date.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2015 journal article
The Development and Characterization of an Exogenous Green-Light-Regulated Gene Expression System in Marine Cyanobacteria
Marine Biotechnology, 17(3), 245–251.
Contributors: A. Badary *, K. Abe *, S. Ferri *, K. Kojima * & *
author keywords: Marine cyanobacteria; Synechococcus; Optogenetics; Green-light-sensing proteins; Synthetic biology
MeSH headings : Aquatic Organisms; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Gene Expression Regulation, Bacterial / radiation effects; Genes, Reporter; Genetic Engineering; Genetic Vectors / chemistry; Genetic Vectors / metabolism; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Histidine Kinase; Light; Promoter Regions, Genetic; Protein Kinases / genetics; Protein Kinases / metabolism; Real-Time Polymerase Chain Reaction; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Synechococcus / genetics; Synechococcus / metabolism; Synechococcus / radiation effects; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription, Genetic / radiation effects
TL;DR:
The constructed green-light-regulated gene expression system was effective at regulating the recombinant expression of a target gene using green light in a marine cyanobacterial strain that does not naturally possess such a green- light regulation system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2015 journal article
X-ray structure of recombinant nonglycosylated FAD glucose dehydrogenase derived from Aspergillus flavus
Febs Journal, 282, 279. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000362570605056&KeyUID=WOS:000362570605056
Source: ORCID
Added: October 9, 2020
2014 journal article
A green-light inducible lytic system for cyanobacterial cells
Biotechnology For Biofuels, 7(1), (9 April 2014).
Contributors: K. Miyake*, K. Abe *, S. Ferri *, M. Nakajima*, M. Nakamura*, W. Yoshida, K. Kojima *, K. Ikebukuro *, *
author keywords: Cyanobacteria; Self-lysis; Two-component system; T4 bacteriophage; Synthetic biology
TL;DR:
The constructed green-light inducible lytic system was clearly induced by green- light illumination, resulting in lytic cells that released intracellular phycocyanin into the culture supernatant, suggesting a future possibility to construct photosynthetic genetically modified organisms that are unable to survive under sunlight exposure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2014 journal article
Effects of Eliminating Pyruvate Node Pathways and of Coexpression of Heterogeneous Carboxylation Enzymes on Succinate Production by Enterobacter aerogenes
Applied and Environmental Microbiology, 81(3), 929–937.
Contributors: Y. Tajima*, Y. Yamamoto *, K. Fukui *, Y. Nishio*, K. Hashiguchi*, Y. Usuda *, *
MeSH headings : Actinobacillus / enzymology; Actinobacillus / genetics; Corynebacterium glutamicum / enzymology; Corynebacterium glutamicum / genetics; Culture Media / chemistry; Enterobacter aerogenes / enzymology; Enterobacter aerogenes / genetics; Enterobacter aerogenes / metabolism; Gene Deletion; Gene Expression; Glucose / metabolism; Hydrogen-Ion Concentration; Metabolic Engineering; Phosphoenolpyruvate Carboxykinase (ATP) / genetics; Phosphoenolpyruvate Carboxykinase (ATP) / metabolism; Pyruvate Carboxylase / genetics; Pyruvate Carboxylase / metabolism; Pyruvic Acid / metabolism; Succinic Acid / metabolism
TL;DR:
Lowering the pH in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs and can be applied to identify additional engineering targets to increase succinate production.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2014 journal article
Electrochemical detection of pathogenic bacteria by using a glucose dehydrogenase fused zinc finger protein
Analytical Methods, 6(14), 4991–4994.
Contributors: J. Lee *, A. Tatsumi*, K. Abe *, W. Yoshida *, * & K. Ikebukuro *
TL;DR:
An electrochemical detection system for pathogenic bacteria by utilizing a glucose dehydrogenase-fused zinc finger protein (ZF-GDH), which could detect PCR products electrochemically without the need for DNA probe hybridization is developed.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2014 journal article
Study of the role of anaerobic metabolism in succinate production by Enterobacter aerogenes
Applied Microbiology and Biotechnology, 98(18), 7803–7813.
Contributors: Y. Tajima*, K. Kaida*, A. Hayakawa*, K. Fukui *, Y. Nishio*, K. Hashiguchi*, R. Fudou, K. Matsui*, Y. Usuda *, *
author keywords: Enterobacter aerogenes; Succinate fermentation; Redox balance; Phosphoenolpyruvate carboxykinase; Ethanol dehydrogenase
TL;DR:
These single-gene knockout studies showed that the ethanol synthesis pathway serves as the dominant NADH re-oxidation pathway in this organism and showed the potential of bacteria-based succinate fermentation under weak acidic conditions.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2014 journal article
THE 3RD GENERATION CGM SENSOR UTILIZING DIRECT ELECTRON TRANSFER PRINCIPLE
Diabetes Technology & Therapeutics, 16, A78–A79. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000331218000205&KeyUID=WOS:000331218000205
Source: ORCID
Added: October 9, 2020
2014 journal article
The development of an autonomous self-powered bio-sensing actuator
Sensors and Actuators B-Chemical, 196, 429–433.
Contributors: T. Hanashi*, T. Yamazaki *, H. Tanaka *, K. Ikebukuro *, W. Tsugawa * & *
author keywords: Enzyme fuel cell; Autonomous; Sensing actuator; Flavin adenine dinucleotide-dependent glucose dehydrogenase; Self-powered; Drug delivery systems
TL;DR:
An autonomous, self-powered, sensing actuator that employs the principle of BioCapacitor as the core technology is reported, which demonstrates its potential for application as a drug delivery/pumping system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2013 journal article
Blood Glucose Dependence on Emotional Behaviors and Body Surface Temperatures in Common Marmoset's Socio-Psychological Learning with Peers - for 'Development of Human-Environment Interface by Sensing and Multivariate Analysis of Bio-Ecosystem'
Bioengineering Based on Electrochemistry, 50(28), 9–14.
Contributors: M. Koshiba *, A. Senoo, G. Karino*, Y. Shirakawa*, K. Mimura *, T. Sagawa*, W. Tsugawa *, * , S. Nakamura *
Source: ORCID
Added: October 9, 2020
2013 journal article
Cloning and Characterization of Fructosamine-6-Kinase from Arthrobacter aurescens
Applied Biochemistry and Biotechnology, 170(3), 710–717.
Contributors: A. Sakaguchi-Mikami*, M. Kameya*, S. Ferri *, W. Tsugawa * & *
author keywords: Glycation; Amadori products; Arthrobacter; epsilon-Fructosyl lysine (epsilon-FK); Fructosamine-6-kinase (FN6K)
MeSH headings : Amino Acid Sequence; Arthrobacter / enzymology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; Escherichia coli / genetics; Lysine / analogs & derivatives; Lysine / metabolism; Molecular Sequence Data; Phosphotransferases (Alcohol Group Acceptor) / genetics; Phosphotransferases (Alcohol Group Acceptor) / metabolism; Sequence Alignment; Substrate Specificity
TL;DR:
The presence of FN6K in Arthrobacter spp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Design of riboregulators for control of cyanobacterial (Synechocystis) protein expression
Biotechnology Letters, 36(2), 287–294.
Contributors: K. Abe *, Y. Sakai*, S. Nakashima*, M. Araki*, W. Yoshida *, * , K. Ikebukuro
author keywords: Biofuels; Cyanobacteria; Post-transcriptional gene regulation; Riboregulator; Synechocystis; Synthetic biology
MeSH headings : Bacterial Proteins / biosynthesis; Biotechnology / methods; Gene Expression Regulation, Bacterial; Metabolic Engineering / methods; Molecular Biology / methods; RNA / metabolism; Ribosomes / drug effects; Ribosomes / metabolism; Synechocystis / genetics; Synechocystis / metabolism
TL;DR:
R riboregulators, that one of the post-transcriptional regulators composed of RNAs, were constructed for controlling cyanobacterial protein expression and were effective in Synechocystis sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2013 journal article
Engineering Fructosyl Peptide Oxidase to Improve Activity Toward the Fructosyl Hexapeptide Standard for HbA1c Measurement
Molecular Biotechnology, 54(3), 939–943.
Contributors: S. Ferri *, Y. Miyamoto *, A. Sakaguchi-Mikami*, W. Tsugawa * & *
author keywords: Fructosyl peptide oxidase; Hemoglobin A1c; Glycated hemoglobin; Substrate specificity; Protein engineering; Dehydrogenase; Oxidase; Diabetes monitoring
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Sequence; Ascomycota / enzymology; Ascomycota / genetics; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Glycated Hemoglobin; Glycopeptides / chemistry; Glycopeptides / metabolism; Models, Molecular; Molecular Sequence Data; Mutation; Oligopeptides / chemistry; Oligopeptides / metabolism; Protein Engineering / methods; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Reference Standards; Substrate Specificity
TL;DR:
The recently discovered fructosyl peptide oxidase from Phaeosphaeria nodorum (PnFPOX) was demonstrated to react with the glycated hexapeptide measurement standard of hemoglobin A1c, fVHLTPE.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Engineering glucose oxidase to minimize the influence of oxygen on sensor response
Electrochimica Acta, 126, 158–161.
Contributors: Y. Horaguchi*, S. Saito *, K. Kojima *, W. Tsugawa *, S. Ferri * & *
author keywords: Biosensor; Glucose oxidase; Glucose dehydrogenase; Flavin adenine dinucleotide; Oxygen
TL;DR:
The rational amino acid substitution approach resulted in the construction of the Ser114Ala/Phe355Leu mutant, which has an 11-fold decrease in oxidase activity and 2.8-fold increase in dehydrogenase activity compared with wild-type GOx and was considerably less affected by oxygen than the wild- type GOx-based sensor at lower glucose concentrations.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Engineering of a green-light inducible gene expression system in Synechocystis sp PCC6803
Microbial Biotechnology, 7(2), 177–183.
Contributors: K. Abe *, K. Miyake*, M. Nakamura*, K. Kojima *, S. Ferri *, K. Ikebukuro *, *
MeSH headings : Artificial Gene Fusion; Bacterial Proteins / genetics; Gene Expression; Gene Expression Regulation, Bacterial / radiation effects; Genes, Reporter; Genetic Engineering; Genetics, Microbial / methods; Green Fluorescent Proteins / analysis; Green Fluorescent Proteins / genetics; Light; Promoter Regions, Genetic; Synechocystis / genetics; Synechocystis / radiation effects; Transcriptional Activation / radiation effects
TL;DR:
The constructed engineered green‐light sensing system resulted in about 40‐fold higher protein expression than with the wild‐type promoter with a high ON/OFF ratio under green‐ light illumination, which would be a useful genetic tool for controlling gene expression in the emergent cyanobacterial bioprocesses.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Identification and functional analysis of fructosyl amino acid-binding protein from Gram-positive bacterium Arthrobacter sp.
Journal of Applied Microbiology, 114(5), 1449–1456.
Contributors: A. Sakaguchi-Mikami*, S. Ferri *, S. Katayama *, W. Tsugawa * & *
author keywords: Arthrobacter; fructosyl amino acid-binding protein; fructosyl amino acid; glycation; Gram-positive bacteria; Substrate-binding proteins
MeSH headings : Amino Acid Sequence; Arthrobacter / genetics; Arthrobacter / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Fatty Acids / metabolism; Fructosamine / metabolism; Ligands; Lipoproteins / genetics; Lipoproteins / metabolism; Membrane Proteins / genetics; Membrane Proteins / metabolism; Molecular Sequence Data; Protein Sorting Signals; Sequence Homology, Amino Acid; Substrate Specificity
TL;DR:
A novel FABP from Gram‐positive bacteria, Arthrobacter spp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2013 journal article
Improving the Gene-Regulation Ability of Small RNAs by Scaffold Engineering in Escherichia coli
Acs Synthetic Biology, 3(3), 152–162.
Contributors: Y. Sakai*, K. Abe *, S. Nakashima *, W. Yoshida *, S. Ferri *, * , K. Ikebukuro *
author keywords: attenuator; Hfq; post-transcriptional gene regulation; riboregulator; small RNA; transcriptional gene regulation
MeSH headings : Base Sequence; Escherichia coli / genetics; Gene Expression Regulation, Bacterial / genetics; Genetic Engineering / methods; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; RNA, Small Untranslated / genetics; Synthetic Biology / methods
TL;DR:
Efficient gene regulation abilities of small RNAs are improved by directly fusing the natural Escherichia coli trans-encoded small RNA-derived scaffolds, including Hfq-binding and rho-independent transcription terminator sequences, to the 3' end of the smallRNAs that mediate RNA-based gene regulation.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Partial Peptide of alpha-Synuclein Modified with Small-Molecule Inhibitors Specifically Inhibits Amyloid Fibrillation of alpha-Synuclein
International Journal of Molecular Sciences, 14(2), 2590–2600.
Contributors: W. Yoshida *, N. Kobayashi*, Y. Sasaki*, K. Ikebukuro * & *
author keywords: alpha-synuclein; amyloid beta; pyrroloquinoline quinone; Baicalein; EGCG
TL;DR:
These achievements provide a novel strategy for the development of targeted specific amyloid formation inhibitors: the combination of quinone compounds with specific peptide sequence from target proteins involved in amyloids formation.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2013 journal article
Screening of Peptide Ligands for Pyrroloquinoline Quinone Glucose Dehydrogenase Using Antagonistic Template-Based Biopanning
International Journal of Molecular Sciences, 14(12), 23244–23256.
Contributors: K. Abe *, W. Yoshida *, K. Terada*, Y. Yagi-Ishii*, S. Ferri *, K. Ikebukuro *, *
author keywords: peptide ligand; phage display; site-directed; inhibitor
MeSH headings : Amino Acid Sequence; Binding Sites; Glucose Dehydrogenases / antagonists & inhibitors; Glucose Dehydrogenases / genetics; Glucose Dehydrogenases / metabolism; Kinetics; Ligands; Mutagenesis, Site-Directed; Peptide Library; Peptides / chemical synthesis; Peptides / chemistry; Peptides / metabolism; Protein Structure, Tertiary; Substrate Specificity
TL;DR:
The results demonstrate that the antagonistic template-based biopanning is useful for screening peptide ligands recognizing the specific local tertiary structure of proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2013 journal article
Simultaneous Improvement of Specificity and Affinity of Aptamers Against Streptococcus mutans by In Silico Maturation for Biosensor Development
Biotechnology and Bioengineering, 111(3), 454–461.
Contributors: N. Savory *, Y. Takahashi*, K. Tsukakoshi *, H. Hasegawa *, M. Takase*, K. Abe *, W. Yoshida *, S. Ferri *
author keywords: aptamers; Streptococcus mutans; Cell-SELEX; in silico maturation; flow-through test
MeSH headings : Aptamers, Nucleotide / isolation & purification; Biosensing Techniques / methods; Computer Simulation; Sensitivity and Specificity; Streptococcus mutans / isolation & purification
TL;DR:
A successful application of ISM of aptamers is demonstrated to simultaneously improve specificity and affinity for Streptococcus mutans with discovery of a core sequence, which was required to form a polymerized guanine quadruplex structure for target binding.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2012 journal article
BioLC-Oscillator: A Self-Powered Wireless Glucose-Sensing System with the Glucose Dependent Resonance Frequency
Electrochemistry, 80(5), 367–370.
Contributors: T. Hanashi*, T. Yamazaki *, W. Tsugawa *, K. Ikebukuro * & *
author keywords: BioCapacitor; Continuous Glucose Monitoring; Glucose Dehydrogenase; Wireless Sensing System
TL;DR:
This study succeeded in constructing a stand-alone, self-powered, wireless glucose sensing system called the BioLC-Oscillator by using a radio transmitter in which the radio wave resonance frequency changes according to glucose concentration within the range from 0.86 to 10.1mM, which covers from the hypoglycemic range to a part of the hyperglycemic range.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2012 journal article
Biomolecular Engineering of Biosensing Molecules - The Challenges in Creating Sensing Molecules for Glycated Protein Biosensing
Electrochemistry, 80(5), 293–298.
Contributors: S. Ferri * & *
author keywords: Biomolecular Engineering; Biosensing; Glycated Proteins; Fructosyl Peptide Oxidase
TL;DR:
Glycated protein sensing is one of the most emergent and focused on technology in the field of diabetes diagnosis and the recent advances in the development of fructosyl amino acid/fructo peptide oxidases are focused on to acknowledge the rewards of the current advanced biomolecular engineering technology.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2012 journal article
Construction of Mutant Glucose Oxidases with Increased Dye-Mediated Dehydrogenase Activity
International Journal of Molecular Sciences, 13(11), 14149–14157.
Contributors: Y. Horaguchi*, S. Saito *, K. Kojima *, W. Tsugawa *, S. Ferri * & *
author keywords: glucose oxidase; glucose dehydrogenase; FAD; oxygen; site directed mutagenesis
MeSH headings : Amino Acid Substitution; Coloring Agents / chemistry; Coloring Agents / metabolism; Glucose Oxidase / chemistry; Glucose Oxidase / genetics; Glucose Oxidase / metabolism; Kinetics; Models, Molecular; Molecular Conformation; Mutation; Oxidation-Reduction; Oxidoreductases / chemistry; Oxidoreductases / metabolism; Oxygen / chemistry; Oxygen / metabolism; Substrate Specificity
TL;DR:
Results indicate that alteration of the dehydrogenase/oxidase activity ratio of GOxs is possible by introducing a mutation into the putative functional residues responsible for oxidative half reaction with oxygen of these enzymes, resulting in a further increased dehydrogen enzyme activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2012 journal article
Detection of Pathogenic Bacteria by Using Zinc Finger Protein Fused with Firefly Luciferase
Analytical Chemistry, 84(18), 8028–8032.
Contributors: K. Abe *, T. Kumagai*, C. Takahashi *, A. Kezuka*, Y. Murakami *, Y. Osawa*, H. Motoki*, T. Matsuo*
MeSH headings : Binding Sites; DNA, Bacterial / analysis; Early Growth Response Protein 1 / genetics; Early Growth Response Protein 1 / metabolism; Escherichia coli / genetics; Escherichia coli / isolation & purification; Legionella pneumophila / genetics; Legionella pneumophila / isolation & purification; Luciferases, Firefly / genetics; Luciferases, Firefly / metabolism; Polymerase Chain Reaction; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Sp1 Transcription Factor / genetics; Sp1 Transcription Factor / metabolism; Zinc Fingers / genetics
TL;DR:
A novel bacterial genome detection system using zinc finger protein fused with firefly luciferase (ZF-luciferase), which succeeded in the detection of 10 copies of the genome of Legionella pneumophila and Escherichia coli O157.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2012 journal article
Direct electron transfer type disposable sensor strip for glucose sensing employing an engineered FAD glucose dehydrogenase
Enzyme and Microbial Technology, 52(2), 123–128.
Contributors: Y. Yamashita *, S. Ferri *, M. Huynh*, H. Shimizu*, H. Yamaoka* & *
author keywords: Diabetes; Glucose dehydrogenase; Glucose sensor; SMBG; Substrate specificity; Direct electron transfer
MeSH headings : Amino Acid Sequence; Amino Acid Substitution; Aspergillus niger / enzymology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biosensing Techniques; Blood Glucose / analysis; Blood Glucose Self-Monitoring / instrumentation; Blood Glucose Self-Monitoring / methods; Burkholderia cepacia / enzymology; Burkholderia cepacia / genetics; Catalytic Domain; Computer Simulation; Electrochemical Techniques; Electron Transport; Escherichia coli; Flavin-Adenine Dinucleotide / metabolism; Fungal Proteins / metabolism; Glucose / metabolism; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Glucose Dehydrogenases / metabolism; Glucose Oxidase / metabolism; Humans; Maltose / metabolism; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Reagent Strips; Recombinant Proteins / metabolism; Sensitivity and Specificity; Substrate Specificity
TL;DR:
The engineered FADGDH was used to develop a direct electron transfer-type, disposable glucose sensor strip by immobilizing the enzyme complex onto a carbon screen-printed electrode and showed a clear glucose concentration-dependent response that was not affected by the presence of maltose.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2012 journal article
Electrochemical SNP Detection Using Glucose Dehydrogenase
Electrochemistry, 80(5), 345–347.
Contributors: K. Abe *, C. Kiyohara*, Y. Saito *, * & K. Ikebukuro *
author keywords: Single Nucleotide Polymorphism; Glucose Dehydrogenase; Single Base Extension
TL;DR:
An electrochemical detection system of an SNP of peroxisome proliferator-activated receptor γ2 (PPARγ2) (C34G) using glucose dehydrogenase (GDH) based on a single base extension method based on amperometric sensing system is constructed.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2012 journal article
Mutational analysis of the oxygen-binding site of cholesterol oxidase and its impact on dye-mediated dehydrogenase activity
Journal of Molecular Catalysis B-Enzymatic, 88, 41–46.
Contributors: K. Kojima *, T. Kobayashi *, W. Tsugawa *, S. Ferri * & *
author keywords: Cholesterol dehydrogenase; Cholesterol oxidase; FAD; Site-directed mutagenesis
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2012 journal article
Nitrous Oxide Sensing using Oxygen-Insensitive Direct-Electron-Transfer-Type Nitrous Oxide Reductase
Electrochemistry, 80(5), 371–374.
Contributors: W. Tsugawa *, H. Shimizu*, M. Tatara*, Y. Ueno *, K. Kojima * & *
author keywords: Nitrous Oxide; Nitrous Oxide Reductase; Direct Electron Transfer; Cytochrome c
Source: ORCID
Added: October 9, 2020
2012 journal article
Rapid Cytotoxicity Screening Platform for Amyloid Inhibitors Using a Membrane-Potential Sensitive Fluorescent Probe
Analytical Chemistry, 85(1), 185–192.
Contributors: J. Kim *, Y. Sasaki*, W. Yoshida *, N. Kobayashi*, A. Veloso*, K. Kerman *, K. Ikebukuro *, *
MeSH headings : Amyloid beta-Peptides / antagonists & inhibitors; Amyloid beta-Peptides / genetics; Amyloid beta-Peptides / metabolism; Antioxidants / chemistry; Barbiturates / chemistry; Biosensing Techniques; Catechin / analogs & derivatives; Catechin / chemistry; Cell Line, Tumor; Cell Survival / drug effects; Flavanones / chemistry; Fluorescent Dyes / chemistry; Humans; Isoxazoles / chemistry; Peptide Fragments / antagonists & inhibitors; Peptide Fragments / genetics; Peptide Fragments / metabolism; Recombinant Proteins / antagonists & inhibitors; Recombinant Proteins / biosynthesis; Recombinant Proteins / toxicity; Thiobarbiturates / chemistry; alpha-Synuclein / antagonists & inhibitors; alpha-Synuclein / genetics; alpha-Synuclein / metabolism
TL;DR:
The development of a rapid cytotoxicity evaluation system using a membrane potential-sensitive bis-oxonol fluorescent dye, DiBAC4(3), and the capacity of well-defined polyphenolic antioxidants to mitigate amyloid-induced cytot toxicity was evaluated using the developed biosensing system.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2012 journal article
Selection of DNA Aptamers That Recognize alpha-Synuclein Oligomers Using a Competitive Screening Method
Analytical Chemistry, 84(13), 5542–5547.
Contributors: K. Tsukakoshi *, K. Abe *, * & K. Ikebukuro *
MeSH headings : Alzheimer Disease / metabolism; Amyloid / chemistry; Amyloid / metabolism; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Base Sequence; Binding, Competitive; Gene Library; Humans; Parkinson Disease / metabolism; Protein Binding; Protein Structure, Secondary; SELEX Aptamer Technique; alpha-Synuclein / chemistry; alpha-Synuclein / metabolism
TL;DR:
The results of this study support the hypothesis that amyloid oligomers share a common structure and may serve as powerful analytical tools for the design and development of drugs and diagnostic tests for neurodegenerative diseases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2012 journal article
Structural Basis of Efficient Electron Transport between Photosynthetic Membrane Proteins and Plastocyanin in Spinach Revealed Using Nuclear Magnetic Resonance
Plant Cell, 24(10), 4173–4186.
Contributors: T. Ueda *, N. Nomoto*, M. Koga*, H. Ogasa*, Y. Ogawa*, M. Matsumoto*, P. Stampoulis*, * , H. Terasawa *, I. Shimada *
MeSH headings : Cytochrome b6f Complex / metabolism; Electron Transport; Hydrophobic and Hydrophilic Interactions; Magnetic Resonance Spectroscopy; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Molecular Sequence Data; Photosynthesis; Photosystem I Protein Complex / metabolism; Plastocyanin / chemistry; Plastocyanin / metabolism; Spinacia oleracea / metabolism
TL;DR:
This work investigates the residues of plastocyanin in close proximity to photosystem I and cytochrome b6f, in both the thylakoid vesicle–embedded and solubilized states to demonstrate the transient characteristics of the interactions on the acidic patch facilitate the rapid association and dissociation of Pc.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2012 journal article
Substrate specificity engineering of Escherichia coli derived fructosamine 6-kinase
Biotechnology Letters, 35(2), 253–258.
Contributors: K. Kojima *, A. Mikami-Sakaguchi*, M. Kameya*, Y. Miyamoto *, S. Ferri *, W. Tsugawa *, *
author keywords: Fructosamine 6-kinase; Fructosyl-valine; Site-directed mutagenesis; Substrate specificity
MeSH headings : Amino Acid Substitution; Catalytic Domain; Escherichia coli / enzymology; Fructosamine / metabolism; Lysine / analogs & derivatives; Lysine / metabolism; Metabolic Engineering; Models, Molecular; Mutagenesis, Site-Directed; Mutant Proteins / genetics; Mutant Proteins / metabolism; Phosphotransferases / genetics; Phosphotransferases / metabolism; Protein Engineering; Substrate Specificity; Valine / analogs & derivatives; Valine / metabolism
TL;DR:
Among the various Met220-substituted mutant enzymes, Met220Leu, which corresponded to the B. subtilis residue, resulted in an increased activity of fructosyl-valine and decreased activity offructo-lysine, thus increasing the specificity for fructsyl-Valine by 40-fold.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2011 journal article
Aptameric sensors based on structural change for diagnosis (vol 149, pg 93, 2011)
Faraday Discussions, 149, 106. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000285924900009&KeyUID=WOS:000285924900009
Source: ORCID
Added: October 9, 2020
2011 journal article
BioRadioTransmitter: A self-powered wireless glucose-sensing system
Journal of Diabetes Science and Technology, 5(5), 1030–1035.
Contributors: T. Hanashi*, T. Yamazaki *, W. Tsugawa *, K. Ikebukuro * & *
MeSH headings : Biosensing Techniques / instrumentation; Blood Glucose / analysis; Equipment Design; Glucose / analysis; Transducers
TL;DR:
A stand-alone, self-powered, wireless glucose-sensing system called a BioRadioTransmitter is constructed by using a radio transmitter in which the radio wave transmission frequency changes with the glucose concentration in the fuel cell.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2011 journal article
Construction of engineered fructosyl peptidyl oxidase for enzyme sensor applications under normal atmospheric conditions
Biotechnology Letters, 34(3), 491–497.
Contributors: S. Kim*, E. Nibe*, W. Tsugawa *, K. Kojima *, S. Ferri * & *
author keywords: Biosensor; Dehydrogenase; Diabetes; Fructosyl peptidyl oxidase (FPOX); Glycated protein; HbA1c
MeSH headings : Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Ascomycota / enzymology; Biosensing Techniques / methods; Glycated Hemoglobin / analysis; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR:
Improvement of dye-mediated dehydrogenase activity of FPOXs and its application to enzyme electrode construction were undertaken to produce an enzyme electrode for the measurement of fructosyl-αN-valyl-histidine (f-αVal-His), the protease-digested product of HbA1c.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2011 journal article
Development of a novel biosensing system based on the structural change of a polymerized guanine-quadruplex DNA nanostructure
Biosensors & Bioelectronics, 26(12), 4837–4841.
Contributors: Y. Morita*, W. Yoshida *, N. Savory *, S. Han*, M. Tera *, K. Nagasawa *, C. Nakamura *, * , K. Ikebukuro *
author keywords: Aptamers; FADGDH; G-quadruplexes; Nanostructures; Biosensors; Enzymes
MeSH headings : Adenosine / analysis; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Biosensing Techniques / methods; G-Quadruplexes; Glucose 1-Dehydrogenase / metabolism; Guanine / chemistry; Nanostructures / chemistry; Nanostructures / ultrastructure; Polymerization
TL;DR:
An adaptor molecule, named the Gq-switch, which links an electrode with flavin adenine dinucleotide-dependent glucose dehydrogenase (FADGDH) that is capable of transferring electron to a electrode directly is developed.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2011 conference paper
ECS Transactions: Preface
ECS Transactions, 35(7). http://www.scopus.com/inward/record.url?eid=2-s2.0-79960806989&partnerID=MN8TOARS
Source: ORCID
Added: October 9, 2020
2011 journal article
Effect of time of cutting and maceration on forage composition, nutrient flow, microbial protein synthesis, and digestibility in dual-flow continuous culture
Canadian Journal of Animal Science, 91(3), 486–487. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000294744800042&KeyUID=WOS:000294744800042
Source: ORCID
Added: October 9, 2020
2011 journal article
Glucose Specific GDH-PQQ based Sensor Strip: Application of Engineered GDH-PQQ Harboring a de novo Designed Loop Region
Bioelectronics, Biointerfaces, and Biomedical Applications 4, 35(7), 117–119.
Contributors: D. Nagae*, M. Nakajima*, S. Ferri * & *
Source: ORCID
Added: October 9, 2020
2011 journal article
Hemoglobin A1c biosensors
Japanese Journal of Clinical Chemistry, 40(4), 304–311. http://www.scopus.com/inward/record.url?eid=2-s2.0-82155186699&partnerID=MN8TOARS
Source: ORCID
Added: October 9, 2020
2011 journal article
Review of glucose oxidases and glucose dehydrogenases: A bird's eye view of glucose sensing enzymes
Journal of Diabetes Science and Technology, 5(5), 1068–1076.
Contributors: S. Ferri *, K. Kojima * & *
MeSH headings : Glucose 1-Dehydrogenase / metabolism; Glucose Oxidase / metabolism; Humans; Oxidation-Reduction; Substrate Specificity
TL;DR:
The present knowledge of redox enzymes currently utilized in commercially available glucose monitoring systems are summarized to promote a fuller appreciation of enzymatic properties and principles employed in blood glucose monitoring to help avoid potential errors.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2011 journal article
Screening of Aspergillus-derived FAD-glucose dehydrogenases from fungal genome database
Biotechnology Letters, 33(11), 2255–2263.
Contributors: K. Mori*, M. Nakajima*, K. Kojima *, K. Murakami*, S. Ferri * & *
author keywords: Aspergillus; FAD; Glucose dehydrogenase; 3D structure
MeSH headings : Amino Acid Motifs; Aspergillus niger / enzymology; Aspergillus niger / genetics; Cloning, Molecular; Coenzymes / metabolism; Computational Biology; Escherichia coli / genetics; Flavin-Adenine Dinucleotide / metabolism; Gene Expression; Genome, Fungal; Glucose Dehydrogenases / genetics; Glucose Dehydrogenases / metabolism; Models, Molecular; Penicillium / enzymology; Penicillium / genetics; Phylogeny; Protein Conformation; Sequence Homology, Amino Acid
TL;DR:
Results suggest that the putative proteins belonging to the second clade of Aspergillus niger and Penicillium amagasakiens GODs are FADGDHs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2011 journal article
Tuning Fructosyl Peptidyl Oxidase into Dehydrogenase and Its Application for the Construction of an Enzyme Electrode
Bioelectronics, Biointerfaces, and Biomedical Applications 4, 35(7), 113–116.
Contributors: S. Ferri *, E. Nibe*, Y. Miyamoto *, S. Kim*, W. Tsugawa * & *
Source: ORCID
Added: October 9, 2020
2010 journal article
Aptameric sensors based on structural change for diagnosis
Faraday Discussions, 149, 93–105.
Contributors: K. Abe *, D. Ogasawara *, W. Yoshida *, * & K. Ikebukuro *
MeSH headings : Aptamers, Nucleotide / chemistry; Base Sequence; Biomarkers / analysis; Biosensing Techniques / methods; Circular Dichroism / methods; Electrophoretic Mobility Shift Assay / methods; Humans; Molecular Sequence Data; Nucleic Acid Conformation; Surface Plasmon Resonance / methods; Thrombin / analysis
TL;DR:
An aptamer-based bound/free (B/F) separation system that uses a designed aptamer named the "capturable aptamer", which changes its structure upon recognizing its target molecule thereby exposing a specific single-strand region, and the potential of capturable aptamers in clinical diagnostics.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Constructing an improved pyrroloquinoline quinone glucose dehydrogenase binding aptamer for enzyme labeling
Biotechnology Letters, 32(9), 1293–1298.
Contributors: K. Abe *, * & K. Ikebukuro *
author keywords: DNA aptamer; Electrochemical biosensors; Pyrroloquinoline quinone-dependent glucose dehydrogenase; G-quadruplex
MeSH headings : Aptamers, Nucleotide / metabolism; Glucose Dehydrogenases / metabolism; Protein Binding; Staining and Labeling / methods
TL;DR:
Because the truncated mutants minimized unexpected interactions between PGa4 and the target- binding aptamer, they will now facilitate the construction of a highly sensitive detection system using a PQQGDH-labeled diagnostic marker-binding aptamer.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Construction of a Novel Glucose-Sensing Molecule Based on a Substrate-Binding Protein for Intracellular Sensing
Biotechnology and Bioengineering, 108(4), 725–733.
Contributors: A. Sakaguchi-Mikami*, A. Taniguchi *, * & T. Yamazaki *
author keywords: intracellular sensing; gene regulation; transcriptional regulator; lactose repressor (LacI); substrate-binding proteins (SBPs); glucose-/galactose-binding protein (GBP)
MeSH headings : Animals; Carrier Proteins / genetics; Carrier Proteins / metabolism; DNA / metabolism; Escherichia coli / genetics; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Gene Expression Regulation; Genes, Reporter; Glucose / analysis; Glucose / metabolism; Hep G2 Cells; Humans; Lac Repressors / genetics; Lac Repressors / metabolism; Mice; Models, Molecular; NIH 3T3 Cells; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Transcription, Genetic; Transfection
TL;DR:
It is demonstrated that SLCPGL functions as a glucose‐sensitive transcriptional regulator in mammalian cells and reveals the possibility of developing an SBP‐based regulator as a probe of intracellular sensing and gene regulation system for mammalian cells in response to a desired ligands depending on the SBP ligand specificity.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Engineering of dye-mediated dehydrogenase property of fructosyl amino acid oxidases by site-directed mutagenesis studies of its putative proton relay system
Biotechnology Letters, 32(8), 1123–1129.
Contributors: S. Kim*, E. Nibe*, S. Ferri *, W. Tsugawa * & *
author keywords: Biosensor; Dehydrogenase; Flavoprotein; Fructosyl amino acid oxidase (FAOD); Glycated protein; Proton relay system (PRS)
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Sequence; Aspergillus / enzymology; Cloning, Molecular; Crystallography, X-Ray; Electrophoresis, Polyacrylamide Gel; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidoreductases / chemistry; Oxidoreductases / genetics; Oxidoreductases / metabolism; Pichia / enzymology; Protons
TL;DR:
To alter their electron acceptor availability, site-directed mutagenesis was carried out on conserved residues predicted to be involved in the proton relay system (PRS) of two eukaryotic FAODs, the FAOD from the marine yeast Pichia sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2010 journal article
Functional expression of Phanerochaete chrysosporium cellobiose dehydrogenase flavin domain in Escherichia coli
Biotechnology Letters, 32(6), 855–859.
Contributors: Desriani, S. Ferri * & *
author keywords: Cellobiose; Cellobiose dehydrogenase; Flavin domain; Phanerochaete chrysosporium
MeSH headings : Carbohydrate Dehydrogenases / biosynthesis; Carbohydrate Dehydrogenases / genetics; Cellobiose / metabolism; Enzyme Stability; Escherichia coli / genetics; Escherichia coli / metabolism; Gene Expression; Hydrogen-Ion Concentration; Phanerochaete / enzymology; Phanerochaete / genetics; Pichia / genetics; Pichia / metabolism; Protein Structure, Tertiary; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics
TL;DR:
The soluble flavin domain of the Phanerochaete chrysosporium CDH was successfully expressed in Escherichia coli and the enzyme showed dye-mediated CDH activity higher than that of the complete CDH, composed of flavindomain and heme domain.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Motif-Based Search for a Novel Fructosyl Peptide Oxidase From Genome Databases
Biotechnology and Bioengineering, 106(3), 358–366.
Contributors: S. Kim*, S. Ferri *, W. Tsugawa *, K. Mori* & *
author keywords: biosensor; diabetes; hemoglobin Alc (HbA1c); fructosyl amino acid oxidase (FAOD); fructosyl peptide oxidase (FPOX)
MeSH headings : Amino Acid Motifs; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Sequence; Ascomycota / enzymology; Ascomycota / genetics; Base Sequence; Cloning, Molecular; Computational Biology / methods; Databases, Nucleic Acid; Escherichia coli; Fungal Proteins / genetics; Genome, Fungal; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sequence Alignment; Substrate Specificity
TL;DR:
The gene encoding a putative fructosyl amino acid oxidase was found in the Phaeosphaeria nodorum SN15 genome and successfully expressed in Escherichia coli, and Gly60 was found to be a determining residue for the activity towards f‐αVal‐His.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Pyrroloquinoline quinone inhibits the fibrillation of amyloid proteins
Prion, 4(1).
Contributors: J. Kim *, M. Kobayashi *, M. Fukuda *, D. Ogasawara *, N. Kobayashi*, S. Han*, C. Nakamura *, M. Inada *
author keywords: amyloid beta; amyloid fibril; cytotoxicity; fibril formation; inhibitor; prion; pyrroloquinoline quinone
MeSH headings : Amyloid / chemistry; Amyloid / metabolism; Animals; Humans; Mice; Molecular Structure; Neurodegenerative Diseases / drug therapy; PQQ Cofactor / chemistry; PQQ Cofactor / pharmacology; PQQ Cofactor / therapeutic use
TL;DR:
Pyrroloquinoline quinone (PQQ) inhibits theAmyloid fibril formation of the amyloid proteins, amyloids β (1-42) and mouse prion protein and this compound may become a leading anti-neurodegeneration compound in the treatment of neurodegenerative diseases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2010 journal article
Pyrroloquinoline quinone inhibits the fibrillation of amyloid proteins
Prion, 4(1), 26–31. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000277180900005&KeyUID=WOS:000277180900005
Contributors: J. Kim, M. Kobayashi, M. Fukuda, D. Ogasawara, N. Kobayashi, S. Han, C. Nakamura, M. Inada
Source: ORCID
Added: October 9, 2020
2010 journal article
Pyrroloquinoline quinone inhibits the fibrillation of amyloid proteins.
Prion, 4(1), 26–31. http://www.scopus.com/inward/record.url?eid=2-s2.0-77949286151&partnerID=MN8TOARS
Contributors: J. Kim, M. Kobayashi, M. Fukuda, D. Ogasawara, N. Kobayashi, S. Han, C. Nakamura, M. Inada
Source: ORCID
Added: October 9, 2020
2010 journal article
Screening and Improvement of an Anti-VEGF DNA Aptamer
Molecules, 15(1), 215–225.
Contributors: Y. Nonaka*, * & K. Ikebukuro *
author keywords: aptamer; cancer diagnosis; sensor element; VEGF(121); VEGF(165)
MeSH headings : Aptamers, Nucleotide / analysis; Aptamers, Nucleotide / genetics; Base Sequence; Circular Dichroism; Humans; Kinetics; Molecular Sequence Data; Mutant Proteins / metabolism; SELEX Aptamer Technique / methods; Surface Plasmon Resonance; Vascular Endothelial Growth Factor A / metabolism
Source: ORCID
Added: October 9, 2020
2010 journal article
Screening of DNA aptamer which binds to alpha-synuclein
Biotechnology Letters, 32(5), 643–648.
Contributors: K. Tsukakoshi *, R. Harada *, * & K. Ikebukuro *
author keywords: alpha-Synuclein; Alzheimer's disease; DNA aptamer; Lewy bodies; Parkinson's disease
MeSH headings : Aptamers, Nucleotide / isolation & purification; Aptamers, Nucleotide / metabolism; Drug Evaluation, Preclinical / methods; Electrophoresis, Polyacrylamide Gel; Electrophoretic Mobility Shift Assay; Humans; Protein Binding; alpha-Synuclein / metabolism
TL;DR:
The first DNA aptamers against α-synuclein using native PAGE applied to the SELEX method are identified and this aptamer is called “M5-15”; it is the α- synuclein-bound aptamer and was isolated after four cycles of screening.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Selection of DNA aptamer against prostate specific antigen using a genetic algorithm and application to sensing
Biosensors & Bioelectronics, 26(4), 1386–1391.
Contributors: N. Savory *, K. Abe *, * & K. Ikebukuro *
author keywords: DNA aptamer; Prostate specific antigen; Genetic algorithm; In silk maturation
MeSH headings : Algorithms; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / isolation & purification; Base Sequence; Biosensing Techniques / methods; Biosensing Techniques / statistics & numerical data; Gene Library; Humans; Male; Nucleic Acid Conformation; Prostate-Specific Antigen / analysis; Prostate-Specific Antigen / blood; Prostatic Neoplasms / blood; Prostatic Neoplasms / diagnosis; SELEX Aptamer Technique
TL;DR:
The first report of a DNA aptamer against PSA and its application to PSA sensing is reported, with the aptamer showing a 48-fold higher PSA-binding ability than candidates obtained by SELEX.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
The inhibitory effect of pyrroloquinoline quinone on the amyloid formation and cytotoxicity of truncated alpha-synuclein
Molecular Neurodegeneration, 5(1).
Contributors: J. Kim *, R. Harada *, M. Kobayashi *, N. Kobayashi* & *
TL;DR:
It is demonstrated that PQQ inhibits the amyloid fibril formation and cytotoxicity of the C-truncated alpha-synuclein variants and is believed to be a strong candidate for a reagent compound in the treatment of PD.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2010 journal article
Wireless monitoring of blood glucose levels in flatfish with a needle biosensor
Fisheries Science, 76(4), 687–694.
Contributors: H. Endo *, E. Takahashi*, M. Murata, H. Ohnuki *, H. Ren*, W. Tsugawa *, *
author keywords: Biosensor; Fish; Glucose; Blood; Interstitial fluid; Flatfish; Monitoring; Determination
TL;DR:
EISF glucose levels were found to be correlated with those in the blood and to be approximately the same as blood glucose levels in the range of 7–25 mg dl−1, and the estimated glucose levels using a one-point calibration method were correlated with actualBlood glucose levels.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2009 journal article
Amino acid substitution at the substrate-binding subsite alters the specificity of the Phanerochaete chrysosporium cellobiose dehydrogenase
Biochemical and Biophysical Research Communications, 391(2), 1246–1250.
Contributors: Desriani, S. Ferri * & *
author keywords: Cellobiose dehydrogenase; Cellobiose; Lactose; Phanerochaete chrysosporium
MeSH headings : Amino Acid Substitution; Binding Sites / genetics; Carbohydrate Dehydrogenases / chemistry; Carbohydrate Dehydrogenases / genetics; Carbohydrate Dehydrogenases / metabolism; Flavins / metabolism; Lactose / metabolism; Phanerochaete / enzymology; Protein Structure, Tertiary; Substrate Specificity / genetics
TL;DR:
While the wild-typecellobiose dehydrogenase efficiently oxidizes cellobiose and lactose, the Glu279Gln mutant retained most of its activity with cellobioses but was completely inactive with lactose.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2009 journal article
An Aptamer-Based Bound/Free Separation System for Protein Detection
Electroanalysis, 21(11), 1297–1302.
Contributors: M. Fukasawa*, W. Yoshida *, H. Yamazaki, * & K. Ikebukuro *
author keywords: Simple bound/free separation; Aptamers; Complementary strand-immobilized (CSI) beads; Structural change of aptamer; PQQGDH
TL;DR:
Aptamer hybridizes with its complementary strand and can be recovered easily and detected by PQQGDH activity, which allows the detection of 270 pM thrombin and 1 nM IgE.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2009 journal article
DNA Aptamers that Bind to PQQGDH as an Electrochemical Labeling Tool
Electroanalysis, 21(11), 1303–1308.
Contributors: Y. Osawa*, M. Takase*, * & K. Ikebukuro *
author keywords: DNA; Aptamers; Electrochemical biosensors; Pyrroquinoline quinone glucose dehydrogenase; Labeling; Aptamer blotting
TL;DR:
It is believed that the PGa4 aptamer against PQQGDH may become a powerful labeling tool for electrochemical biosensors.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2009 journal article
Kinetic Mechanism and Inhibitor Characterization of WNK1 Kinase
Biochemistry, 48(43), 10255–10266.
Contributors: Y. Yagi*, K. Abe *, K. Ikebukuro * & *
MeSH headings : Electrophoretic Mobility Shift Assay; Enzyme Inhibitors / pharmacology; Humans; Inhibitory Concentration 50; Intracellular Signaling Peptides and Proteins; Kinetics; Minor Histocompatibility Antigens; Protein Serine-Threonine Kinases / antagonists & inhibitors; Protein Serine-Threonine Kinases / metabolism; Pyrazoles / pharmacology; Pyrimidines / pharmacology; Staurosporine / pharmacology; Tyrphostins / pharmacology; WNK Lysine-Deficient Protein Kinase 1
TL;DR:
The development of an in vitro WNK1 microfluidic mobility shift assay for kinetic mechanism studies is described and it is concluded that the compound-based structural alignment enabled us to find interesting relationships among the kinases.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2009 journal article
Review of fructosyl amino acid oxidase engineering research: A glimpse into the future of hemoglobin A1c biosensing
Journal of Diabetes Science and Technology, 3(3), 585–592.
Contributors: S. Ferri *, S. Kim*, W. Tsugawa * & *
MeSH headings : Amino Acid Oxidoreductases; Bioengineering / trends; Biosensing Techniques; Blood Glucose / metabolism; Diabetes Mellitus / blood; Glycated Hemoglobin / analysis; Humans
TL;DR:
This work reviews the available FAOD-based assays for measurement of glycated proteins as well as the recent advances and future direction of FAOD research, expected to lead to the next generation of convenient, simple, and economical sensors for glycated protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2009 journal article
The construction of a glucose-sensing luciferase
Biosensors & Bioelectronics, 25(1), 76–81.
Contributors: A. Taneoka*, A. Sakaguchi-Mikami*, T. Yamazaki *, W. Tsugawa * & *
author keywords: Biosensor; Bioluminescence; Glucose sensing; Glucose-sensing molecule; Galactose-/glucose-binding protein; GGBP; Split luciferases
MeSH headings : Biosensing Techniques / methods; Escherichia coli / genetics; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Galactose / analysis; Glucose / analysis; Luciferases / genetics; Luciferases / metabolism; Luminescent Measurements / methods; Monosaccharide Transport Proteins / genetics; Monosaccharide Transport Proteins / metabolism; Mutation; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Sensitivity and Specificity
TL;DR:
The results demonstrate that the GGBP fused with a split luciferase, which is reconstituted rapidly and specifically in the presence of glucose, provides a novel glucose-sensing system based on luminescence and may also contribute to the construction of luminescent-based sensing molecules for other substrates using other PBPs.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2009 journal article
The effect of amino acid substitution in the imperfect repeat sequences of alpha-synuclein on fibrillation
Biochimica Et Biophysica Acta-Molecular Basis of Disease, 1792(10), 998–1003.
Contributors: R. Harada, N. Kobayashi, J. Kim, C. Nakamura *, S. Han*, K. Ikebukuro, *
author keywords: Parkinson's disease; alpha-Synuclein; Fibrillation; E46K; Imperfect repeat sequences
MeSH headings : Amino Acid Sequence; Amino Acid Substitution / genetics; Circular Dichroism; Humans; Light; Microscopy, Atomic Force; Molecular Sequence Data; Mutant Proteins / chemistry; Mutant Proteins / ultrastructure; Propanols / pharmacology; Protein Structure, Quaternary; Protein Structure, Secondary; Repetitive Sequences, Amino Acid; Scattering, Radiation; alpha-Synuclein / chemistry; alpha-Synuclein / ultrastructure
TL;DR:
Observed mutations in the imperfect KTKE(Q)GV repeat sequences of the N-terminal region were prone to decrease the lag time of fibril formation, and AFM imaging suggested that the Q24K mutant formed twisted fibrils, while the other mutants formed spherical aggregates and short fibrILS.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2009 journal article
The inhibition of amyloid fibrillation using the proteolytic products of PQQ-modified α-synuclein
Open Biotechnology Journal, 3, 40–45.
Contributors: N. Kobayashi*, J. Kim *, K. Ikebukuro * & *
TL;DR:
PQQ-modified peptide fragments, the proteolytic products of PQZ-modified � - synuclein, prevent the amyloid formation of full-length � -synuclein and these inhibitory effects are derived from the PQQ modification of the peptide.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2009 journal article
Uniform molecularly imprinted microspheres and nanoparticles prepared by precipitation polymerization: The control of particle size suitable for different analytical applications (vol 584, pg 112, 2007)
Analytica Chimica Acta, 657(2), 215.
Contributors: K. Yoshimatsu *, K. Reimhult, A. Krozer, K. Mosbach*, * & L. Ye *
Source: ORCID
Added: October 9, 2020
2009 journal article
Zn finger-based direct detection system for PCR products of Salmonella spp. and the Influenza A virus
Biotechnology Letters, 31(5), 735.
Contributors: Y. Osawa*, K. Ikebukuro *, T. Kumagai*, H. Motoki*, T. Matsuo*, M. Horiuchi *, *
Source: ORCID
Added: October 9, 2020
2009 journal article
Zn finger-based direct detection system for PCR products of Salmonella spp. and the Influenza A virus
Biotechnology Letters, 31(5), 725–733.
Contributors: Y. Osawa*, K. Ikebukuro * & *
author keywords: Influenza A virus; PCR products; Salmonella spp.; Zn finger protein
MeSH headings : DNA Gyrase / genetics; DNA, Bacterial / analysis; DNA, Viral / analysis; Early Growth Response Protein 1 / metabolism; Influenza A virus / isolation & purification; Nucleocapsid Proteins; Polymerase Chain Reaction / methods; RNA-Binding Proteins / genetics; Salmonella / isolation & purification; Sp1 Transcription Factor / metabolism; Viral Core Proteins / genetics
TL;DR:
This work has detected PCR products from Salmonella spp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Aggregation and fibrillation study of a-synuclein under applied voltage
Electrochemistry, 76(8), 614–618.
Contributors: Y. Osawa*, K. Ikebukuro *, N. Kobayashi*, S. Han*, C. Nakamura * & *
author keywords: aggregation; application of voltage; alpha-synuclein; natively unfolded protein
TL;DR:
Voltage is applied to amyloidogenic protein, α-synuclein, which is related to Parkinson’s disease to control the aggregation of α- Syn, which has a negative charge, by applying voltage to it, demonstrating that this technique might be useful not only to efficiently control aggregation ofα-syn but also to understand the mechanism of aggregation and fibrillation of α.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
BioCapacitor-A novel category of biosensor
Biosensors & Bioelectronics, 24(7), 1837–1842.
Contributors: T. Hanashi*, T. Yamazaki *, W. Tsugawa *, S. Ferri *, D. Nakayama *, M. Tomiyama*, K. Ikebukuro *, *
author keywords: Biofuel cells; Glucose dehydrogenase; Continuous glucose monitoring; Glucose sensor; Capacitor; Wireless sensing system
MeSH headings : Biosensing Techniques / instrumentation; Electric Capacitance; Electrochemistry / instrumentation; Electrodes; Enzymes, Immobilized / chemistry; Equipment Design; Equipment Failure Analysis; Glucose / analysis; Glucose / chemistry; Glucose 1-Dehydrogenase / chemistry; Reproducibility of Results; Sensitivity and Specificity
TL;DR:
A BioCapacitor employing FAD-dependent glucose dehydrogenase (FADGDH) as its anodic catalyst can be operated as a self-powered enzyme sensor.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2008 journal article
Biofuel cell system employing thermostable glucose dehydrogenase
Biotechnology Letters, 30(10), 1753–1758.
Contributors: J. Okuda-Shimazaki *, N. Kakehi*, T. Yamazaki *, M. Tomiyama* & *
author keywords: biofuel cell; glucose dehydrogenase; direct electron transfer
MeSH headings : Bioelectric Energy Sources; Burkholderia cepacia / genetics; Catalysis; Enzyme Stability; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
TL;DR:
The results indicate the potential application of this thermostable glucose dehydrogenase for the construction of a compartment-less biofuel cell.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 book
Biosensors Using the Aptameric Enzyme Subunit: The Use of Aptamers in the Allosteric Control of Enzymes
In Aptamers in Bioanalysis (pp. 129–138).
Contributors: K. Ikebukuro *, W. Yoshida * & *
Source: ORCID
Added: October 9, 2020
2008 journal article
Construction and Characterization of Direct Electron Transfer-Type Continuous Glucose Monitoring System Employing Thermostable Glucose Dehydrogenase Complex
Analytical Letters, 41(13), 2363–2373.
Contributors: T. Yamazaki *, J. Okuda-Shimazaki *, C. Sakata*, T. Tsuya* & *
author keywords: Burkhorderia cepacia glucose dehydrogenase; continuous glucose monitoring (CGM); cytochrome c; direct electron transfer; glucose sensor
TL;DR:
A direct electron transfer-type enzyme electrode using thermostable FAD-glucose dehydrogenase consisting of three distinct subunits and its application in developing a continuous glucose monitoring (CGM) system without a synthetic electron mediator is demonstrated.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Cumulative effect of amino acid substitution for the development of fructosyl valine-specific fructosyl amine oxidase
Enzyme and Microbial Technology, 44(1), 52–56.
Contributors: S. Kim*, S. Miura*, S. Ferri *, W. Tsugawa * & *
author keywords: Biosensor; Diabetes; Fructosyl amine oxidase; Hemoglobin A(1c) (HbA(1c)); Substrate specificity
TL;DR:
The results support the proposed 3D model, and the resulting f-αVal-specific mutants are expected to be applied to the enzymatic measurement of HbA1c.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Detection system based on the conformational change in an aptamer and its application to simple bound/free separation
Biosensors & Bioelectronics, 24(5), 1372–1376.
Contributors: D. Ogasawara *, N. Hachiya *, K. Kaneko*, * & K. Ikebukuro *
author keywords: Aptamer; Conformational change; Bound/free separation; Prion protein
MeSH headings : Aptamers, Nucleotide / chemistry; Binding Sites; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Equipment Design; Equipment Failure Analysis; Prions / chemistry; Prions / ultrastructure; Protein Binding; Protein Conformation; Reproducibility of Results; Sensitivity and Specificity; Surface Plasmon Resonance
TL;DR:
This work designed aptamers which change their conformation upon binding to the target molecule and thereby expose a single-strand bearing the complementary sequence to the capture probe immobilized onto the support, which allows for simple bound/free separation with only one target ligand.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Development of fructosyl amine oxidase specific to fructosyl valine by site-directed mutagenesis
Protein Engineering Design & Selection, 21(4), 233–239.
Contributors: S. Miura*, S. Ferri *, W. Tsugawa *, S. Kim * & *
author keywords: active site; biosensor; docking; fructosyl amine oxidase; substrate specificity
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / isolation & purification; Amino Acid Oxidoreductases / metabolism; Asparagine / metabolism; Bacillus / enzymology; Buffers; Glycated Hemoglobin / metabolism; Lysine / analogs & derivatives; Lysine / metabolism; Models, Biological; Mutagenesis, Site-Directed / methods; Mutation; Pichia / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sarcosine Oxidase / chemistry; Sarcosine Oxidase / metabolism; Substrate Specificity; Valine / analogs & derivatives; Valine / metabolism
TL;DR:
This work has provided the first structural model of substrate binding with eukaryotic FAOD, which is expected to contribute to further investigation of FAOD.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2008 journal article
Engineering of ligand specificity of periplasmic binding protein for glucose sensing
Biotechnology Letters, 30(8), 1453–1460.
Contributors: A. Sakaguchi-Mikami*, A. Taneoka*, R. Yamoto*, S. Ferri * & *
author keywords: biosensor; blood glucose monitoring; fluorescence; galactose; glucose-binding protein (GGBP); periplasmic binding protein
MeSH headings : Aspartic Acid / genetics; Binding Sites; Biosensing Techniques / methods; Escherichia coli; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / metabolism; Galactose / metabolism; Genetic Engineering; Glucose / metabolism; Ligands; Monosaccharide Transport Proteins / chemistry; Monosaccharide Transport Proteins / metabolism; Mutant Proteins / metabolism; Periplasmic Binding Proteins / chemistry; Periplasmic Binding Proteins / metabolism; Phenylalanine / genetics; Spectrometry, Fluorescence
TL;DR:
It is demonstrated that it is possible to construct a GGBP which specifically recognizes glucose and has a higher Kd value and use it as a molecular recognition element of blood glucose monitoring systems by combining two different mutations based on the 3D structure of G GBP.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Improvement of aptamer affinity by dimerization
Sensors, 8(2), 1090–1098.
Contributors: H. Hasegawa *, K. Taira*, * & K. Ikebukuro *
author keywords: aptamer; dimerization; avidity
TL;DR:
It is demonstrated that the dimerization of aptamer effectively improves the affinities of those aptamers for their targets.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Nanostructure fabrication based on engineered α-synuclein
Nanobiotechnology, 4(1-4), 50–55.
Contributors: N. Kobayashi*, S. Han*, C. Nakamura * & *
TL;DR:
This paper demonstrates nanomaterial fabrication by utilizing two peptide fragments of the non-amyloid-β component of Alzheimer's disease amyloid region, which is the key region for α-synuclein fibrillation and confirmed that these two peptides individually formed different rod-like structures.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Operational condition of a molecular imprinting catalyst-based fructosyl-valine sensor
Electrochemistry, 76(8), 590–593.
Contributors: T. Yamazaki *, S. Ohta* & *
author keywords: glycated hemoglobin; fructosyl amino acid; molecular imprinting catalyst; artificial dehydrogenase; amperometric sensor
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2008 journal article
Propionate sensor using coenzyme-A transferase and acyl-CoA oxidase
Protein and Peptide Letters, 15(8), 779–781.
Contributors: * , W. Tsugawa, M. Aoyagi, E. Rajashekhara & K. Watanabe *
author keywords: propionate; volatile fatty acid; propionate CoA transferase; short-chain acyl-CoA oxidase; amperometry; enzyme sensor
MeSH headings : Acyl-CoA Oxidase / metabolism; Arabidopsis / enzymology; Biosensing Techniques / methods; Clostridium / enzymology; Coenzyme A-Transferases / metabolism; Electrochemistry; Propionates / metabolism
TL;DR:
An amperometric propionate sensor was developed using comprised of two recombinant enzymes, Propionate coenzyme A CoA transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Selection of DNA aptamers against VEGF(165) using a protein competitor and the aptamer blotting method
Biotechnology Letters, 30(5), 829–834.
Contributors: H. Hasegawa *, * & K. Ikebukuro *
author keywords: aptamer blotting; cancer diagnosis; competitor; DNA aptamer; sensor element; VEGF165
MeSH headings : Aptamers, Nucleotide / isolation & purification; Aptamers, Nucleotide / metabolism; Base Sequence; Binding, Competitive; Biomarkers, Tumor / metabolism; Enzymes, Immobilized / metabolism; Gene Library; Humans; Molecular Probe Techniques; Nucleic Acid Conformation; SELEX Aptamer Technique / methods; Surface Plasmon Resonance; Vascular Endothelial Growth Factor A / metabolism
TL;DR:
Two DNA aptamers against a tumor marker protein, human vascular endothelial growth factor (VEGF165) were identified and would be useful sensor elements for cancer diagnosis by means of aptamer blotting and the affinities of the enriched library were evaluated.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Selection of DNA aptamers against insulin and construction of an aptameric enzyme subunit for insulin sensing
Biosensors & Bioelectronics, 24(5), 1116–1120.
Contributors: W. Yoshida *, E. Mochizuki*, M. Takase*, H. Hasegawa *, Y. Morita*, H. Yamazaki, * , K. Ikebukuro *
author keywords: Insulin-binding aptamer; G-quartet library; Aptameric enzyme subunit; Insulin detection
MeSH headings : Aptamers, Nucleotide / chemistry; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Equipment Design; Equipment Failure Analysis; Insulin / analysis; Insulin / chemistry; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence / instrumentation; Spectrometry, Fluorescence / methods
TL;DR:
An aptameric enzyme subunit (AES) was developed by connecting the best identified insulin-binding aptamer with the thrombin-inhibiting aptamer and was able to detect insulin by measuring theThrombin enzymatic activity without bound/free separation.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Site directed mutagenesis studies of FAD-dependent glucose dehydrogenase catalytic subunit of Burkholderia cepacia
Biotechnology Letters, 30(11), 1967–1972.
Contributors: H. Yamaoka*, Y. Yamashita *, S. Ferri * & *
author keywords: diabetes; glucose dehydrogenase; glucose sensor; substrate specificity
MeSH headings : Alanine / genetics; Amino Acid Sequence; Asparagine / genetics; Base Sequence; Burkholderia cepacia / enzymology; Burkholderia cepacia / genetics; Burkholderia cepacia / metabolism; Catalytic Domain / genetics; Flavin-Adenine Dinucleotide / metabolism; Glucose / metabolism; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed / methods; Mutation; Sequence Homology, Amino Acid; Substrate Specificity
TL;DR:
Two residues are mutated, Asn475 and Ala472, which are located near the putative active site of the catalytic subunit of FADGDH and have been predicted from the alignment with theactive site of glucose oxidase, which showed a higher specificity toward glucose compared to the wild type enzyme.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
The simple and rapid detection of specific PCR products from bacterial genomes using Zn finger proteins
Nucleic Acids Research, 36(11).
Contributors: Y. Osawa*, K. Ikebukuro *, H. Motoki *, T. Matsuo*, M. Horiuchi * & *
MeSH headings : Bacteria / genetics; Bacteria / isolation & purification; Binding Sites; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Enzyme-Linked Immunosorbent Assay; Genome, Bacterial; Humans; Legionella pneumophila / genetics; Polymerase Chain Reaction / methods; Sequence Alignment; Sequence Analysis, DNA; Sp1 Transcription Factor / chemistry; Sp1 Transcription Factor / metabolism; Zinc Fingers
TL;DR:
Results indicate that the rapid and simple detection of PCR products specific to L. pneumophila using a Zn finger protein was achieved and can be applied to the detection of other bacteria using various Zn Finger proteins that have already been reported.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Wireless enzyme sensor system for real-time monitoring of blood glucose levels in fish
Biosensors & Bioelectronics, 24(5), 1417–1423.
Contributors: H. Endo *, Y. Yonemori*, K. Hibi*, H. Ren*, T. Hayashi*, W. Tsugawa *, *
author keywords: Biosensor; Enzyme sensor; Glucose; Blood; Wireless; Telemetory; Real-time; Monitoring; Fish
MeSH headings : Animals; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Blood Chemical Analysis / instrumentation; Blood Chemical Analysis / methods; Blood Glucose / analysis; Blood Glucose / chemistry; Computer Systems; Electrochemistry / instrumentation; Equipment Design; Equipment Failure Analysis; Fishes / metabolism; Glucose Oxidase / chemistry; Monitoring, Ambulatory / instrumentation; Monitoring, Ambulatory / methods; Reproducibility of Results; Sensitivity and Specificity; Telemetry / instrumentation; Telemetry / methods
TL;DR:
A wireless enzyme sensor system to determine blood glucose levels in fish can be rapidly and conveniently monitored using the newly developed needle-type enzyme sensor, consisting of a Pt-Ir wire, Ag/AgCl paste, and glucose oxidase.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2008 journal article
Zinc finger protein-based detection system of PCR products for pathogen diagnosis.
Nucleic Acids Symposium Series (2004), (52), 23–24.
Contributors: Y. Osawa*, H. Motoki *, T. Matsuo*, M. Horiuchi *, * & K. Ikebukuro *
MeSH headings : Bacteria / isolation & purification; DNA-Binding Proteins / metabolism; Influenza A virus / genetics; Influenza A virus / isolation & purification; Legionella pneumophila / genetics; Legionella pneumophila / isolation & purification; Molecular Diagnostic Techniques; Polymerase Chain Reaction / methods; Salmonella / genetics; Salmonella / isolation & purification; Sp1 Transcription Factor / metabolism; Viruses / isolation & purification; Zinc Fingers
TL;DR:
This methodology can be applied to the detection of most pathogen using various Zinc finger proteins and succeeded in detecting the PCR products from Legionella pneumophila, Salmonella spp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
Aptameric enzyme subunit for homogeneous DNA sensing
Biotechnology Letters, 30(2), 243–252.
Contributors: K. Ikebukuro *, W. Yoshida * & *
author keywords: aptameric enzyme subunit; enzyme-inhibiting aptamer; homogeneous DNA sensing
MeSH headings : Aptamers, Nucleotide / pharmacology; Base Sequence; Biosensing Techniques; DNA / analysis; DNA / genetics; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Protein Subunits / antagonists & inhibitors; Protein Subunits / chemistry; Protein Subunits / metabolism; Thrombin / analysis; Thrombin / antagonists & inhibitors; Thrombin / genetics
TL;DR:
An aptameric enzyme subunit (AES) which can detect the DNA in a homogeneous solution that is composed of an enzyme-inhibiting aptamer bearing a target-molecule binding site and was able to detect target DNAs by measuring the thrombin activity in ahomogeneous solution.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
Aptameric enzyme subunit for homogeneous protein sensing.
Nucleic Acids Symposium Series (2004), (51), 99–100. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:18029605&KeyUID=MEDLINE:18029605
Source: ORCID
Added: October 9, 2020
2007 journal article
Branched-chain amino acid biosensing using fluorescent modified engineered leucine/isoleucine/valine binding protein
International Journal of Molecular Sciences, 8(6), 513–525.
Contributors: S. Chino*, A. Sakaguchi*, R. Yamoto*, S. Ferri * & *
author keywords: periplasmic binding proteins (PBPs); biosensor; fluorescence; leucine/isoleucine/valine-binding protein (LIVBP); branched-chain amino acid (BCAA)
TL;DR:
Its high sensitivity and group-specific molecular recognition ability make the new sensing system ideally suited for the measurement of BCAAs and the determination of the Fischer ratio, an indicator of hepatic disease involving metabolic dysfunction.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
Construction of target molecule sensing system using aptameric enzyme subunit based on PQQGDH activity.
Nucleic Acids Symposium Series (2004), (51), 401–402. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:18029756&KeyUID=MEDLINE:18029756
Source: ORCID
Added: October 9, 2020
2007 journal article
Development of a compact stacked flatbed reactor with immobilized high-density bacteria for hydrogen production
International Journal of Hydrogen Energy, 33(5), 1593–1597.
Contributors: M. Ishikawa *, S. Yamamura *, R. Ikeda *, Y. Takamura *, * , E. Tamiya *, M. Tomiyama*
author keywords: bio-hydrogen; flatbed bioreactor; cell immobilization
TL;DR:
A compact stacked flatbed reactor (CSFR) composed of several pieces of agar plates containing an H 2 -producing bacterium, Escherichia coli, was developed to enable the easy extraction of products from the reactor.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
Effect of reparation of repeat sequences in the human α-synuclein on fibrillation ability
International Journal of Biological Sciences, 3(1), 1–7.
MeSH headings : Amino Acid Motifs / genetics; Circular Dichroism; Humans; Mutagenesis, Site-Directed; Mutation; Parkinson Disease / genetics; Parkinson Disease / metabolism; Protein Folding; Protein Structure, Secondary; alpha-Synuclein / chemistry; alpha-Synuclein / genetics; alpha-Synuclein / physiology
TL;DR:
Resistance to formation in the partially folded intermediate may repress the folding of α-synuclein, consequently interfering with the aggregation and fibril formation, and demonstrated that KTKEGV repeats may have a significant role in keeping native unfolded status of α.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
In silico panning for a non-competitive peptide inhibitor
Bmc Bioinformatics, 8.
Contributors: Y. Yagi*, K. Terada*, T. Noma *, K. Ikebukuro * & *
MeSH headings : Acinetobacter calcoaceticus / enzymology; Allosteric Site / genetics; Binding, Competitive / genetics; Combinatorial Chemistry Techniques / methods; Directed Molecular Evolution / methods; Glucose 1-Dehydrogenase / antagonists & inhibitors; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Ligands; Peptide Library; Peptides / antagonists & inhibitors; Peptides / genetics; Peptides / metabolism; Protein Binding / genetics; Protein Structure, Secondary / genetics; Surface Plasmon Resonance
TL;DR:
This study demonstrates an effective methodology of in silico panning for the selection of a non-competitive peptide inhibitor from small virtual peptide library and highlights the usefulness of this strategy for structure-based screening of enzyme inhibitors.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
Label-free homogeneous detection of immunoglobulin E by an aptameric enzyme subunit
Biotechnology Letters, 30(3), 421–425.
Contributors: W. Yoshida *, * & K. Ikebukuro *
author keywords: aptameric enzyme subunit; enzyme-inhibiting aptamer; protein detection; immunoglobulin E
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Base Sequence; Biosensing Techniques / methods; Humans; Immunoglobulin E / analysis; Thrombin / antagonists & inhibitors
TL;DR:
An aptameric enzyme subunit (AES) for immunoglobulin E (IgE) sensing is developed and it was able to detect IgE by using this AES in homogeneous solution with a detection limit of 50 pmol.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
Methanogenesis from acetate and propionate by thermophilic down-flow anaerobic packed-bed reactor
Bioresource Technology, 99(11), 4786–4795.
author keywords: anaerobic digestion; propionate; methanogenic microllora; packed-bed reactor; syntroph
MeSH headings : Acetates / metabolism; Anaerobiosis; Archaea / genetics; Bacteria / genetics; Bioreactors; DNA, Ribosomal / analysis; Fatty Acids / metabolism; Gene Library; Genes, Archaeal; Methane / metabolism; Oxygen / metabolism; Phylogeny; Propionates / metabolism; RNA, Ribosomal, 16S / analysis; Temperature; Volatilization
TL;DR:
The experimental results indicate that the packed-bed design can maintain a sufficiently high density of methanogenic microorganisms within the system even at reduced HRTs as well as facilitate an efficient degradation of propionate and acetate, possibly through syntrophic reactions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2007 journal article
Peptide ligand screening of alpha-synuclein aggregation modulators by in silico panning
Bmc Bioinformatics, 8.
Contributors: K. Abe *, N. Kobayashi*, * & K. Ikebukuro *
MeSH headings : Amino Acid Sequence; Binding Sites; Computer Simulation; Dimerization; Drug Design; Ligands; Models, Chemical; Models, Molecular; Molecular Sequence Data; Multiprotein Complexes / chemistry; Multiprotein Complexes / ultrastructure; Peptides / chemistry; Protein Binding; Protein Conformation; Sequence Analysis, Protein / methods
TL;DR:
It is demonstrated that it is possible to screen α-synuclein-binding peptides by in silico panning, and the screened peptides bind to α- Synuclein, thus affecting the aggregation of α- synuclein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2007 journal article
SPCE based glucose sensor employing novel thermostable glucose dehydrogenase, FADGDH: Blood glucose measurement with 150nL sample in one second
Journal of Diabetes Science and Technology, 1(1), 28–35.
TL;DR:
The simplicity of the construction and the high precision of this FADGDH-based glucose biosensor makes it an alternative to previously reported commercially available glucose sensors designed for the SMBG.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
Screening of DNA aptamer against mouse prion protein by competitive selection.
Prion, 1(4), 248–254.
Contributors: D. Ogasawara *, H. Hasegawa *, K. Kaneko*, * & K. Ikebukuro *
author keywords: aptamer; DNA; SELEX; competitive selection; prion protein; beta-PrP; detection
MeSH headings : Animals; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / metabolism; Aptamers, Nucleotide / pharmacology; Brain / metabolism; Brain / pathology; Drug Evaluation, Preclinical; Humans; Mice; Prion Diseases / drug therapy; Prion Diseases / metabolism; Prion Diseases / pathology; Prions / antagonists & inhibitors; Prions / chemistry; Prions / metabolism; Protein Structure, Tertiary
TL;DR:
Clone 4-9 might be a useful tool in prion-disease diagnosis and research because it was indicated that clone 4- 9 could recognize the flexible region of the N-terminal domain of PrP.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2007 journal article
Selection and characterization of DNA aptamers against VEGF165 with aptamer blotting method and its application.
Nucleic Acids Symposium Series (2004), (51), 399–400.
Contributors: K. Ikebukuro *, H. Hasegawa * & *
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / isolation & purification; Aptamers, Nucleotide / metabolism; Base Sequence; Binding, Competitive; Biomarkers, Tumor / metabolism; Humans; Molecular Probe Techniques; Molecular Sequence Data; Nucleic Acid Conformation; SELEX Aptamer Technique; Vascular Endothelial Growth Factor A / metabolism
TL;DR:
Two DNA aptamers against a tumor marker protein, human vascular endothelial growth factor (VEGF165), using another protein as a competitor in the SELEX process allowed us to select the specific aptamer against a target protein efficiently.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2007 journal article
Selection of DNA aptamers that inhibit enzymatic activity of PQQGDH and its application.
Nucleic Acids Symposium Series (2004), (51), 403–404. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:18029757&KeyUID=MEDLINE:18029757
Source: ORCID
Added: October 9, 2020
2007 journal article
Specific detection of PCR product from Legionella pneumophila strain Philadelphia1 using zinc finger protein Sp2.
Nucleic Acids Symposium Series (2004), (51), 285–286. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:18029698&KeyUID=MEDLINE:18029698
Source: ORCID
Added: October 9, 2020
2007 journal article
W-motif exchange between beta-propeller proteins
Protein Journal, 26(3), 153–158.
author keywords: beta-propeller protein; W-motif; motif exchange; chimeric enzyme; molecular evolution
MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Catalytic Domain; Glucose Dehydrogenases / chemistry; Models, Biological; Molecular Sequence Data; Neuraminidase / chemistry; Protein Engineering; Protein Folding; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Structure-Activity Relationship
TL;DR:
The similarity between the structural scaffold of PQQGDH and that of sialidase in the absence of any similarity in the primary structure, catalytic function and substrate recognition encouraged us to attempt a W-motif exchange between these enzymes.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
A novel wireless glucose sensor employing direct electron transfer principle based enzyme fuel cell
Biosensors & Bioelectronics, 22(9-10), 2250–2255.
Contributors: N. Kakehi*, T. Yamazaki *, W. Tsugawa * & *
author keywords: glucose dehydrogenase; continuous glucose monitoring; bio-fuel cell; glucose sensor; direct electron transfer
MeSH headings : Animals; Biosensing Techniques / instrumentation; Burkholderia cepacia / enzymology; Cattle; Glucose / analysis; Glucose 1-Dehydrogenase
TL;DR:
This is the first demonstration of an enzyme-based direct electron transfer-type enzyme fuel cell and fuel cell-type glucose sensor which can be utilized as a subcutaneously implantable system for continuous glucose monitoring.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2006 journal article
A screening method for DNA aptamers that bind to specific, unidentified protein in tissue samples
Biotechnology Letters, 28(17), 1377–1381.
Contributors: T. Noma *, K. Ikebukuro *, * , T. Ohkubo*, Y. Sakasegawa *, N. Hachiya *, K. Kaneko*
author keywords: aptamer; electrophoresis blotting; multiple target proteins; systematic evolution of ligands by exponential enrichment
MeSH headings : Animals; Aptamers, Nucleotide / metabolism; Chickens; Gene Library; Muscle, Skeletal / metabolism; Oligonucleotides / metabolism; Protein Binding; Proteomics / methods
TL;DR:
To select aptamers that bind to a specific unidentified protein in tissues for protein analysis, a screening method was developed using chicken skeletal muscle as a model, suggesting this screening method might be useful for selecting aptamer for proteome analysis.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Active site analysis of fructosyl amine oxidase using homology modeling and site-directed mutagenesis
Biotechnology Letters, 28(23), 1895–1900.
Contributors: S. Miura*, S. Ferri *, W. Tsugawa *, S. Kiin & *
author keywords: biosensor; diabetes; fructosyl amine oxidase; HbA1c; substrate specificity
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Sequence; Bacillus / enzymology; Binding Sites; Biotechnology / methods; Diabetes Mellitus / metabolism; Glycated Hemoglobin / chemistry; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Pichia / enzymology; Protein Conformation; Sarcosine Oxidase / chemistry; Sequence Homology, Amino Acid; Substrate Specificity
TL;DR:
A three-dimensional structural model of fructosyl amine oxidase from the marine yeast Pichia N1-1 was generated using the crystal structure of monomeric sarcosine oxidation from Bacillus sp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Analysis of the evolution of the thrombin-inhibiting DNA aptamers using a genetic algorithm
Biotechnology Letters, 28(23), 1933–1937.
Contributors: K. Ikebukuro *, W. Yoshida *, T. Noma * & *
author keywords: aptamer; genetic algorithm; G-quartet structure; inhibitor; systematic; evolution of ligands by exponential enrichment
MeSH headings : Algorithms; Aptamers, Nucleotide; Biotechnology / methods; Chemistry, Physical / methods; DNA / chemistry; Fibrinogen / chemistry; Humans; Ligands; Models, Genetic; Molecular Conformation; Mutation; Nucleic Acid Conformation; RNA / chemistry; Thrombin / antagonists & inhibitors; Thrombin / chemistry
TL;DR:
This method randomized the sequences of the duplex region of this thrombin-inhibiting DNA aptamer and carried out selection based on inhibitory activity using a genetic algorithm, and obtained ligands with greater inhibitory activities than that of the original aptamer.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Aptameric enzyme subunit for biosensing based on enzymatic activity measurement
Analytical Chemistry, 78(10), 3296–3303.
Contributors: W. Yoshida *, * & K. Ikebukuro *
MeSH headings : Adenosine / chemistry; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Base Sequence; Biosensing Techniques / methods; Calibration; Circular Dichroism; Nucleic Acid Conformation; Protease Inhibitors / chemistry; Protease Inhibitors / metabolism; Protein Subunits / antagonists & inhibitors; Protein Subunits / chemistry; Protein Subunits / metabolism; Thrombin / antagonists & inhibitors; Thrombin / metabolism
TL;DR:
The aptameric enzyme subunit (AES), which is a DNA aptamer composed of an enzyme-inhibiting aptamer and a target molecule-binding aptamer, has been developed for the biosensing of target molecules and adenosine was successfully detected by measuring the thrombin activity in a homogeneous solution without bound/free separation.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Characterization and application of aptamers for Taq DNA polymerase selected using an evolution-mimicking algorithm
Biotechnology Letters, 28(23), 1939–1944.
Contributors: T. Noma *, * & K. Ikebukuro *
author keywords: aptamer; evolution-mimicking algorithm; hot-start PCR
MeSH headings : Algorithms; Aptamers, Nucleotide; Base Sequence; Biotechnology / methods; DNA, Viral / genetics; Evolution, Molecular; HIV-1 / genetics; Ligands; Models, Genetic; Molecular Sequence Data; Mutation; Oligonucleotides / chemistry; Polymerase Chain Reaction; Taq Polymerase / chemistry; Taq Polymerase / genetics
TL;DR:
This study characterized the most potent aptamer and demonstrated its applicability and improved the specificity of detection of a low-copy-number target gene in PCR using these DNA polymerases.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Development of a compact high-density microbial hydrogen reactor for portable bio-fuel cell system
International Journal of Hydrogen Energy, 31(11), 1484–1489.
Contributors: M. Ishikawa *, S. Yamamura *, Y. Takamura *, * , E. Tamiya * & M. Tomiyama*
author keywords: biohydrogen; bioreactor; cell immobilization; bio-fuel cell
TL;DR:
The encapsulation of hydrogen producing bacterium, Escherichia coli strain MC13-4, in alginate gel beads provided approximately three-fold increase in hydrogen production in comparison with the free cell suspension.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2006 journal article
Homogeneous DNA sensing using enzyme-inhibiting DNA aptamers
Biochemical and Biophysical Research Communications, 348(1), 245–252.
Contributors: W. Yoshida *, * & K. Ikebukuro *
author keywords: homogeneous DNA sensing; enzyme-inhibiting DNA aptamers; aptameric enzyme subunit; SARS detection; thrombin-inhibiting aptamer
MeSH headings : Aptamers, Peptide; Base Sequence; Biosensing Techniques; DNA / analysis; DNA / genetics; Humans; Molecular Sequence Data; Nucleic Acid Conformation; Protein Subunits / analysis; Sequence Analysis, DNA / methods; Thrombin / analysis; Thrombin / antagonists & inhibitors; Thrombin / genetics
TL;DR:
A novel aptameric enzyme subunit (AES) which can detect target DNAs has been developed and was able to detect targetDNAs by measuring the thrombin activity in a homogeneous solution without bound/free separation, and the lower detection limit was 20nM.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Novel fluorescent sensing system for alpha-fructosyl amino acids based on engineered fructosyl amino acid binding protein
Biosensors & Bioelectronics, 22(9-10), 1933–1938.
Contributors: A. Sakaguchi*, S. Ferri *, W. Tsugawa * & *
author keywords: diabetes; hemoglobin A1c (HbA1c); sensing system; alpha-fructosyl amino acid; periplasmic binding proteins (PBPs); fructosyl amino acid binding protein (FABP)
MeSH headings : Amino Acids / analysis; Amino Acids / chemistry; Biosensing Techniques; Carrier Proteins; Fluorescence; Fructose / chemistry; Protein Engineering; Substrate Specificity
TL;DR:
Its high sensitivity and specificity for alpha-substituted fructosyl amino acids makes the new sensing system ideally suited for the measurement of hemoglobin A1c (HbA1c), a major marker of diabetes.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Production of hydrogen and methane from organic solid wastes by phase-separation of anaerobic process
Bioresource Technology, 98(9), 1861–1865.
Contributors: Y. Ueno *, M. Tatara*, H. Fukui *, T. Makiuchi*, M. Goto * & *
author keywords: anaerobic digestion; hydrogen fermentation; methane fermentation; organic solid wastes; packed-bed reactor
MeSH headings : Anaerobiosis; Bacteria, Anaerobic / physiology; Biodegradation, Environmental; Bioreactors; Evaluation Studies as Topic; Fermentation; Hydrogen / metabolism; Hydrogen-Ion Concentration; Methane / biosynthesis; Organic Chemicals / chemistry; Organic Chemicals / metabolism; Phase Transition; Refuse Disposal / methods
TL;DR:
Phase-separated two-stage anaerobic process was examined and evaluated using artificial organic solid waste in laboratory scale and hydrogenogenic operation was more suitable to combine methanogenic process than solublizing operation, obtaining almost the same levels of overall removal efficiency in both COD and VSS.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Pyrroloquinoline quinone (PQQ) prevents fibril formation of alpha-synuclein
Biochemical and Biophysical Research Communications, 349(3), 1139–1144.
Contributors: M. Kobayashi *, J. Kim *, N. Kobayashi*, S. Han*, C. Nakamura *, K. Ikebukuro *, *
author keywords: Parkinson's disease; alpha-synuclein; pyrroloquinoline quinone; amyloid fibril; thioflavin T
MeSH headings : Amyloid / metabolism; Microscopy, Atomic Force; Molecular Structure; PQQ Cofactor / chemistry; PQQ Cofactor / pharmacology; Protein Binding; Spectrum Analysis; alpha-Synuclein / metabolism; alpha-Synuclein / ultrastructure
TL;DR:
PQQ prevents the amyloid fibril formation and aggregation of alpha-synuclein in vitro in a PQQ-concentration-dependent manner and is a candidate for future anti-PD reagent compounds.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2006 journal article
The application of engineered glucose dehydrogenase to a direct electron-transfer-type continuous glucose monitoring system and a compartmentless biofuel cell
Analytical Letters, 40(3), 431–440.
Contributors: J. Okuda*, T. Yamazaki *, M. Fukasawa*, N. Kakehi* & *
author keywords: fuel cells; glucose dehydrogenase; continuous glucose monitoring; direct electron transfer; cytochrome c domain
TL;DR:
A direct electron‐transfer‐type CGM system employing quinohemoprotein glucose dehydrogenase showed sufficient current response and high operational stability, and was successfully constructed a compartmentless biofuel cell employing QH‐GDH.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2006 journal article
Uniform molecularly imprinted microspheres and nanoparticles prepared by precipitation polymerization: The control of particle size suitable for different analytical applications
Analytica Chimica Acta, 584(1), 112–121.
Contributors: K. Yoshimatsu *, K. Reimhult, A. Krozer, K. Mosbach*, * & L. Ye *
author keywords: molecular imprinting; precipitation polymerization; propranolol; assay; nanoparticles
MeSH headings : Chemistry Techniques, Analytical / methods; Indicators and Reagents; Microchemistry / methods; Microscopy, Electron, Scanning / methods; Microspheres; Nanoparticles / chemistry; Nanotechnology; Propane; Propranolol; Radioligand Assay / methods; Vinyl Compounds
TL;DR:
New synthetic conditions are initiated to obtain MIP beads with controllable size in the nano- to micro-meter range, using racemic propranolol as a model template, and the imprinted sites displayed high chiral selectivity.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2006 journal article
Volatile fatty acid-sensing system involving coenzyme-A transferase
Biotechnology Progress, 22(1), 334–337.
MeSH headings : Animals; Arabidopsis / chemistry; Arabidopsis / enzymology; Biosensing Techniques / methods; Clostridium / chemistry; Clostridium / enzymology; Coenzyme A-Transferases / chemistry; Coenzyme A-Transferases / genetics; Fatty Acids, Volatile / analysis; Fatty Acids, Volatile / chemistry; Propionates / analysis; Propionates / blood; Rabbits; Recombinant Proteins / chemistry; Recombinant Proteins / genetics
TL;DR:
A VFA biosensing system comprised of two recombinant enzymes, propionate coenzyme A (CoA) transferase (PCT) from Clostridium propionicum and acyl‐CoA oxidase from Arabidopsis thaliana, which enabled very sensitive detection of VFAs (down to 1 μM propionates, more than 100‐fold more sensitive compared to previously developed ACS biosensors).
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2005 journal article
Cloning and functional expression of glucose dehydrogenase complex of Burkholderia cepacia in Escherichia coli
Journal of Biotechnology, 123(2), 127–136.
Contributors: T. Tsuya*, S. Ferri *, M. Fujikawa*, H. Yamaoka* & *
author keywords: electron transfer subunit; gene cluster; glucose dehydrogenase; respiration
MeSH headings : Amino Acid Sequence; Burkholderia cepacia / enzymology; Burkholderia cepacia / genetics; Cell Respiration / physiology; Cloning, Molecular / methods; Enzyme Activation; Escherichia coli / physiology; Glucose / metabolism; Glucose 1-Dehydrogenase / analysis; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / genetics; Glucose 1-Dehydrogenase / metabolism; Molecular Sequence Data; Protein Engineering / methods; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism
TL;DR:
The deduced beta subunit amino acid sequence contains three typical heme-binding motifs and was 44-49% identical to the cytochrome c subunits of other FAD-dependent dehydrogenase complexes.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2005 journal article
Construction of artificial cyclic amide amidohydrolases using molecular imprinting technique
Biotechnology Letters, 27(7), 471–475.
Contributors: Z. Meng *, T. Yamazaki * &
author keywords: artificial enzyme; creatininase; cyclic amide amidohydrolase; hydantoinase; molecular imprinting
MeSH headings : Amidohydrolases / chemical synthesis; Amidohydrolases / chemistry; Amidohydrolases / metabolism; Catalysis; Creatinine / metabolism; Hydantoins / metabolism; Molecular Mimicry; Molecular Structure; Polymethacrylic Acids / chemistry; Polymethacrylic Acids / metabolism; Vinyl Compounds / chemistry; Vinyl Compounds / metabolism
TL;DR:
A general molecular imprinting approach is proposed to synthesize artificial enzymes to mimic the family of cyclic amide amidohydrolases which share similar active site and catalytic mechanism.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2005 journal article
Development of a novel sensing probe using DNA aptamer inhibiting enzymatic activity.
Nucleic Acids Symposium Series (2004), (49), 83–84. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:17150644&KeyUID=MEDLINE:17150644
Source: ORCID
Added: October 9, 2020
2005 journal article
Engineered alpha-synuclein prevents wild type and familial Parkin variant fibril formation
Biochemical and Biophysical Research Communications, 335(2), 432–436.
author keywords: alpha-synuclein; amyloid fibril; site-directed mutagenesis; natively unfolded protein; Parkinson's disease
MeSH headings : Alzheimer Disease / metabolism; Amino Acid Sequence; Amyloid / chemistry; Bone Marrow Cells / cytology; Circular Dichroism; DNA / chemistry; DNA, Complementary / metabolism; Gene Library; Humans; Light; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Nerve Tissue Proteins / chemistry; Nerve Tissue Proteins / genetics; Nerve Tissue Proteins / physiology; Parkinson Disease / metabolism; Peptides / chemistry; Polymerase Chain Reaction; Proline / chemistry; Protein Binding; Protein Conformation; Protein Engineering; Protein Structure, Secondary; Scattering, Radiation; Synucleins; Time Factors; Ubiquitin-Protein Ligases / chemistry; Ubiquitin-Protein Ligases / genetics; Ultraviolet Rays; Valine / chemistry; alpha-Synuclein
TL;DR:
Mixing the Val70Thr/Val71Thr double mutant, which has inherently low potential, with the fibril forming α-synucleins, WT and Ala53Thr, greatly reduced their fibrils formation and aggregation and has great potential for further therapeutic approaches.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2005 journal article
Increasing stability of water-soluble PQQ glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface
BMC Biochemistry, 6.
Contributors: S. Tanaka*, S. Igarashi*, S. Ferri * & *
MeSH headings : Actinobacteria / genetics; Actinobacteria / metabolism; Dimerization; Enzyme Stability / physiology; Glucose Dehydrogenases / genetics; Glucose Dehydrogenases / metabolism; Hydrophobic and Hydrophilic Interactions; Solubility; Surface Properties; Water
TL;DR:
Through rational design based on the quaternary structure of the enzyme, residues located at the dimer interface that do not contribute to the intersubunit interaction are selected and the thermal stability of PQQGDH-B was increased without decreasing its catalytic activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2005 journal article
Isolation and characterization of a fructosyl-amine oxidase from an Arthrobacter sp.
Biotechnology Letters, 27(1), 27–32.
Contributors: S. Ferri *, A. Sakaguchi*, H. Goto *, W. Tsugawa * & *
author keywords: Arthrobacter; biosensor; diabetes; fructosyl-amine oxidase; hemoglobin A1c
MeSH headings : Amino Acid Oxidoreductases / chemistry; Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / isolation & purification; Amino Acid Oxidoreductases / metabolism; Amino Acid Sequence; Animals; Arthrobacter / enzymology; Base Sequence; Biosensing Techniques; Escherichia coli / genetics; Molecular Sequence Data; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sequence Homology, Amino Acid
Source: ORCID
Added: October 9, 2020
2005 journal article
Screening of DNA aptamers against multiple proteins in tissue.
Nucleic Acids Symposium Series (2004), (49), 357–358. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:17150781&KeyUID=MEDLINE:17150781
Source: ORCID
Added: October 9, 2020
2005 journal article
SocA is a novel periplasmic binding protein for fructosyl amino acid
Biochemical and Biophysical Research Communications, 336(4), 1074–1080.
Contributors: A. Sakaguchi*, S. Ferri * & *
author keywords: glycation; diabetes; fructosyl amino acid; sensor; bacterial periplasmic binding protein; SocA; Agrobacterium tumefaciens; conformational change; autofluorescence
MeSH headings : Agrobacterium tumefaciens / metabolism; Amino Acid Sequence; Amino Acids / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / isolation & purification; Bacterial Proteins / metabolism; Cloning, Molecular; Escherichia coli / genetics; Escherichia coli / metabolism; Fluorescence; Fructose / metabolism; Molecular Sequence Data; Periplasmic Binding Proteins / chemistry; Periplasmic Binding Proteins / isolation & purification; Periplasmic Binding Proteins / metabolism; Protein Binding; Recombinant Proteins / chemistry; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Substrate Specificity
TL;DR:
It is demonstrated that SocA is a novel FA bPBP that can be utilized as a novel molecular recognition element for the monitoring of FA.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2004 journal article
A molecularly imprinted catalyst designed by a computational approach in catalysing a transesterification process
Biosensors & Bioelectronics, 20(6), 1068–1075.
Contributors: Z. Meng *, T. Yamazaki * & *
author keywords: artificial enzymes; lipase; molecular imprinting; molecular modelling; transesterification
MeSH headings : Acetates / chemistry; Catalysis; Computer-Aided Design; Enzyme Activation; Esterification; Isoenzymes / chemical synthesis; Isoenzymes / chemistry; Lipase / chemical synthesis; Lipase / chemistry; Methacrylates / chemistry; Models, Chemical; Models, Molecular; Substrate Specificity
TL;DR:
A computational approach was developed to optimize the monomer formulation of molecularly imprinted catalysts, and a virtual library of the intermediates of a lipase-catalysed transesterification process was constructed using Chem3D software with p-nitrophenyl acetate as substrate.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2004 journal article
Cloning and expression of fructosyl-amine oxidase from marine yeast Pichia species N1-1
Marine Biotechnology, 6(6), 625–632.
Contributors: S. Ferri *, S. Miura*, A. Sakaguchi*, F. Ishimura*, W. Tsugawa * & *
author keywords: fructosyl-amine oxidase; marine yeast; Pichia
MeSH headings : Amino Acid Oxidoreductases / genetics; Amino Acid Oxidoreductases / metabolism; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cluster Analysis; DNA Primers; Escherichia coli; Gene Expression; Molecular Sequence Data; Phylogeny; Pichia / enzymology; Pichia / genetics; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Sequence Analysis, DNA; Sequence Homology
TL;DR:
The gene encoding the fructosyl-amine oxidase (FAOD) from the marine yeast Pichia sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2004 journal article
Construction and characterization of heterodimeric, soluble quinoprotein glucose dehydrooenase
Journal of Biochemical and Biophysical Methods, 61(3), 331–338.
author keywords: pyrroloquinoline quinone (PQQ); glucose dehydrogenase (GDH); heterodimer cooperativity
MeSH headings : Amino Acid Substitution; Dimerization; Enzyme Activation; Glucose Dehydrogenases / analysis; Glucose Dehydrogenases / chemistry; Multiprotein Complexes / analysis; Multiprotein Complexes / chemistry; Mutagenesis, Site-Directed; Recombinant Proteins / analysis; Recombinant Proteins / chemistry; Solubility; Structure-Activity Relationship
TL;DR:
An effective method of creating heterodimeric PQQGDH-B composed of native (wild-type) and inactive mutant (His168Gln) subunits, which showed slightly decreased GDH activity and almost identical substrate specificity profile to the wild-type enzyme.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2004 journal article
Development of a novel DNA sensing system using DNA aptamer inhibited enzymatic activity 1.
Nucleic Acids Symposium Series (2004), (48), 231–232. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:17150563&KeyUID=MEDLINE:17150563
Source: ORCID
Added: October 9, 2020
2004 journal article
Development of a novel DNA sensing system using DNA aptamer that inhibits enzymatic activity 2.
Nucleic Acids Symposium Series (2004), (48), 309–310. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:17150602&KeyUID=MEDLINE:17150602
Source: ORCID
Added: October 9, 2020
2004 journal article
Development of a novel glucose enzyme fuel cell system employing protein engineered PQQ glucose dehydrogenase
Biosensors & Bioelectronics, 20(10), 2145–2150.
Contributors: N. Yuhashi*, M. Tomiyama*, J. Okuda*, S. Igarashi*, K. Ikebukuro * & *
author keywords: enzyme fuel cell; PQQ glucose dehydrogenase; protein engineering; bilirubin oxidase; stability
MeSH headings : Acinetobacter calcoaceticus / enzymology; Acinetobacter calcoaceticus / genetics; Amino Acid Substitution; Electric Power Supplies; Electrochemistry / instrumentation; Electrochemistry / methods; Energy Transfer; Equipment Design; Equipment Failure Analysis; Feasibility Studies; Glucose / chemistry; Glucose 1-Dehydrogenase / chemistry; Glucose 1-Dehydrogenase / genetics; Mutagenesis, Site-Directed; Pilot Projects; Protein Engineering / methods
TL;DR:
This work constructed the biofuel cell system employing the engineered PQQGDH as the bioanode enzyme and bilirubin oxidase (BOD) as the biocathode, and compared the stability of the bio fuel cell with that employing wild-type PQZGDH.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2004 journal article
Electrochemical detection of protein using a double aptamer sandwich
Analytical Letters, 37(14), 2901–2909.
Contributors: K. Ikebukuro *, C. Kiyohara* & *
author keywords: thrombin; aptamer; sandwich system; electrochemical detection
TL;DR:
An electrochemical sensor system for protein detection using two different antithrombin aptamers that recognize different parts of the target protein and thrombin was detected using a sandwich format.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2004 journal article
Engineered PQQ glucose dehydrogenase based enzyme sensor for continuous glucose monitoring
Analytical Letters, 37(9), 1847–1857.
author keywords: continuous glucose monitoring (CGM); PQQ glucose dehydrogenase (PQQGDH); glucose sensor; cytochrome b(562) (cyt b(562))
TL;DR:
The engineered PQQGDH Ser415Cys has a far superior thermal stability over the wild‐type enzyme, and the applicability of cyt b 562 as the electron mediator to construct a CGM system free of synthetic mediator, achieved the successful application in continuous operation without a significant decrease in the sensor signal.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
1. No Poverty
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2004 journal article
Engineering PQQ glucose dehydrogenase with improved substrate specificity site-directed mutagenesis studies on the active center of PQQ glucose dehydrogenase
Biomolecular Engineering, 21(2), 81–88.
Contributors: S. Igarashi*, T. Hirokawa * & *
author keywords: pyrroloquinoline quinone (PQQ); glucose dehydrogenase (GDH); substrate specificity; protein engineering
MeSH headings : Amino Acid Sequence; Amino Acid Substitution / genetics; Escherichia coli; Glucose / chemistry; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Engineering; Protein Structure, Tertiary; Structure-Activity Relationship; Substrate Specificity / genetics
TL;DR:
Site-directed mutagenesis was carried out on the active site of water-soluble PQQ glucose dehydrogenase (PQQGDH-B) to improve its substrate specificity, and of the numerous Asp167 mutants characterized, Asp 167Glu showed the best substrate specificity profile, while retaining most of its catalytic activity for glucose and stability.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2004 journal article
Essential role of the small subunit of thermostable glucose dehydrogenase from Burkholderia cepacia
Biotechnology Letters, 26(22), 1757–1761.
Contributors: H. Yamaoka*, S. Ferri * & M. Sode *
author keywords: chaperone; flavoenzyme; glucose dehydrogenase; twin-arginine translocase
MeSH headings : Amino Acid Sequence; Burkholderia cepacia / enzymology; Burkholderia cepacia / genetics; Enzyme Activation; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Glucose 1-Dehydrogenase / analysis; Glucose 1-Dehydrogenase / biosynthesis; Glucose 1-Dehydrogenase / chemistry; Molecular Sequence Data; Molecular Weight; Protein Subunits / chemistry; Protein Subunits / metabolism; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism; Structure-Activity Relationship; Temperature
TL;DR:
The co-expression in Escherichia coli of the γ-sub unit and the catalytic α-subunit of the thermostable glucose dehydrogenase (GDH) from Burkholderia cepacia sp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2004 journal article
High-rate thermophilic methane fermentation on short-chain fatty acids in a down-flow anaerobic packed-bed reactor
Bioprocess and Biosystems Engineering, 27(2), 105–113.
author keywords: thermophilic methane fermentation; short-chain fatty acids; short HRTs; high organic loading rate; supporting media
MeSH headings : Acetic Acid / metabolism; Bacteria, Anaerobic / growth & development; Bacteria, Anaerobic / metabolism; Bioreactors / microbiology; Butyric Acid / metabolism; Cell Culture Techniques / methods; Euryarchaeota / growth & development; Euryarchaeota / metabolism; Fatty Acids, Volatile / metabolism; Fermentation / physiology; Methane / metabolism
TL;DR:
In order to maximize the efficiency of methane fermentation on short-chain fatty acids, growth media containing acetic acid and butyric acid as major carbon sources were supplied to a thermophilic down-flow anaerobic packed-bed reactor.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2004 journal article
Highly sensitive detection of DNA hybridization using enzyme electrode
Electrochemistry, 72(8), 598–600. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000223239200012&KeyUID=WOS:000223239200012
Source: ORCID
Added: October 9, 2020
2004 journal article
Molecular engineering of PQQGDH and its applications
Archives of Biochemistry and Biophysics, 428(1), 52–63.
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; DNA / analysis; Enzyme Activation; Enzyme Stability; Glucose / analysis; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Glucose Dehydrogenases / metabolism; Mutagenesis, Site-Directed; PQQ Cofactor / chemistry; PQQ Cofactor / genetics; PQQ Cofactor / metabolism; Protein Conformation; Protein Engineering / methods; Structure-Activity Relationship; Substrate Specificity
Source: ORCID
Added: October 9, 2020
2004 journal article
Novel electrochemical sensor system for protein using the aptamers in sandwich manner
Biosensors & Bioelectronics, 20(10), 2168–2172.
Contributors: K. Ikebukuro *, C. Kiyohara* & *
author keywords: aptamer; sandwich manner; electrochemical detection; pyrroquinoline quinone glucose dehydrogenase ((PQQ)GDH)
MeSH headings : Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Electrochemistry / instrumentation; Electrochemistry / methods; Equipment Design; Equipment Failure Analysis; Humans; Immunoassay / instrumentation; Immunoassay / methods; Protein Binding; Protein Interaction Mapping / instrumentation; Protein Interaction Mapping / methods; Thrombin / analysis; Thrombin / chemistry
TL;DR:
The batch type protein sensing system was constructed using the two different aptamers sandwichingThrombin and it showed linear response to the increase of the thrombin concentration in the range of 40-100 nM.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2004 journal article
PQQ glucose dehydrogenase with novel electron transfer ability
Biochemical and Biophysical Research Communications, 314(3), 793–797.
author keywords: PQQ glucose dehydrogenase; cytochrome; electron transfer; glucose sensor; direct electron transfer type sensor
MeSH headings : Acinetobacter calcoaceticus / enzymology; Alcohol Oxidoreductases / chemistry; Alcohol Oxidoreductases / genetics; Alcohol Oxidoreductases / metabolism; Biosensing Techniques / methods; Comamonas testosteroni / enzymology; Cytochromes c / genetics; Electrodes; Electron Transport; Glucose / analysis; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Glucose Dehydrogenases / metabolism; Kinetics; Protein Engineering; Recombinant Fusion Proteins / genetics; Spectrophotometry / methods
TL;DR:
The constructed fusion protein showed not only intra-molecular electron transfer, between PQQ and heme of the cytochrome c domain, but also electron transfer from heme to the electrode, thereby allowing the construction of a direct electron transfer-type glucose sensor.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2004 journal article
The molecular reaction vessels for a transesterification process created by molecular imprinting technique
Journal of Molecular Recognition, 18(3), 262–266.
Contributors: Z. Meng * & *
author keywords: molecular imprinting; molecular reaction vessel; molecular recognition; polymeric catalyst; transesterification
MeSH headings : Catalysis; Esterification; Hexanols / chemistry; Imidazoles; Methacrylates / chemistry; Microspheres; Nitrophenols / chemistry; Polymers / chemical synthesis; Polymers / chemistry; Succinates / chemistry
TL;DR:
A polymeric catalyst was synthesized by co‐polymerizing 4(5)‐vinylimidazole and itaconic acid with trimethylpropanol trimethacrylate micro spheres with p‐nitrophenyl acetate as template, and molecular imprinting process enhanced the νmax of the Polymeric catalyst 3‐fold.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2003 journal article
Amperometric Glucose Sensor Using Thermostable Co-Factor Binding Glucose Dehydrogenase
IEEJ Transactions on Sensors and Micromachines, 123(6), 185–189.
Contributors: Y. Nakazawa*, T. Yamazaki *, W. Tsugawa *, K. Ikebukuro * & *
TL;DR:
The cross-linked GDH immobilized electrode showed good storage stability and the flow injection analysis (FIA) glucose sensing system was also constructed by immobilizing the cross- linked GDH and ferrocene as a mediator in the carbon paste matrix.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2003 journal article
Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli
Biochimica Et Biophysica Acta-Proteins and Proteomics, 1645(2), 133–138.
Contributors: K. Inose*, M. Fujikawa*, T. Yaniazaki, K. Kojima * & *
author keywords: glucose dehydrogenase; thermal stability; catalytic subunit; FAD
MeSH headings : Amino Acid Sequence; Burkholderia cepacia / enzymology; Burkholderia cepacia / genetics; Catalytic Domain / genetics; Enzyme Stability; Escherichia coli / metabolism; Gene Expression Regulation, Enzymologic; Glucose 1-Dehydrogenase; Glucose Dehydrogenases / biosynthesis; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Molecular Sequence Data; Recombinant Proteins / biosynthesis; Sequence Alignment; Temperature
TL;DR:
A 1620-nucleotide gene encoding the catalytic subunit (alpha subunit) of a thermostable glucose dehydrogenase (GDH) from Burkholderia cepacia is cloned, showing maximum dye-mediated GDH activity at 70 degrees C, retaining high thermal stability.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2003 journal article
Construction of a molecular imprinting catalyst using target analogue template and its application for an amperometric fructosylamine sensor
Biosensors & Bioelectronics, 18(12), 1485–1490.
Contributors: * , S. Ohta*, Y. Yanai* & T. Yamazaki *
author keywords: molecularly imprinted polymer; hemoglobin A1c; amperometric sensor; fructosylamine compound; analogue compound
MeSH headings : Allylamine / chemistry; Biomimetic Materials / chemical synthesis; Biomimetic Materials / chemistry; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Catalysis; Coated Materials, Biocompatible / chemical synthesis; Coated Materials, Biocompatible / chemistry; Crystallization / methods; Electrochemistry / instrumentation; Electrochemistry / methods; Enzymes, Immobilized / chemical synthesis; Enzymes, Immobilized / chemistry; Oxidoreductases Acting on CH-NH Group Donors / chemical synthesis; Oxidoreductases Acting on CH-NH Group Donors / chemistry; Reproducibility of Results; Sensitivity and Specificity; Valine / analogs & derivatives; Valine / analysis; Valine / chemistry
TL;DR:
This work attempted to construct a Fru-val-oxidizing molecularly imprinted catalyst (MIC) utilizing the analogue molecule methyl valine (m-val) as template molecule utilizing allylamine as the functional monomer and m-val as the template molecule to produce an even better MIC-immobilized electrode.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2003 journal article
Development of highly-sensitive fructosyl-valine enzyme sensor employing recombinant fructosyl amine oxidase
Electrochemistry, 71(6), 442–445. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000183761100019&KeyUID=WOS:000183761100019
Source: ORCID
Added: October 9, 2020
2003 journal article
Electrochemical DNA sensor using genetically engineered thermostable pyrroloquinoline quinone glucose dehydrogenase
Electrochemistry, 71(6), 490–495. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000183761100029&KeyUID=WOS:000183761100029
Source: ORCID
Added: October 9, 2020
2003 journal article
Enhancement of the catalytic activity of an artificial phosphotriesterase using a molecular imprinting technique
Biotechnology Letters, 25(13), 1075–1080.
Contributors: Z. Meng *, T. Yamazaki &
author keywords: artificial enzyme; imidazole; molecular imprinting; Paraoxon; phosphotriesterase
MeSH headings : Adsorption; Aryldialkylphosphatase; Biomimetic Materials / chemical synthesis; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Catalysis; Electrochemistry / instrumentation; Electrochemistry / methods; Enzymes, Immobilized / chemical synthesis; Esterases / chemical synthesis; Esterases / chemistry; Imidazoles / chemistry; Methacrylates / chemistry; Paraoxon / chemistry; Pesticides / analysis; Pesticides / chemistry; Quality Control; Vinyl Compounds / chemistry; Zinc / chemistry
TL;DR:
An artificial phosphotriesterase (PTE) was constructed by co-polymerization of 4(5)-vinylimidazole-Zn2+-methacrylic acid cluster with a divinylbenzene polymer and caused 105-fold rate acceleration towards the hydrolysis of diethyl p-nitrophenyl phosphate (Paraoxon).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2003 journal article
Glucose enzyme electrode using cytochrome b(562) as an electron mediator
Biosensors & Bioelectronics, 18(5-6), 699–704.
author keywords: glucose enzyme sensor; electron transfer; Escherichia coli cytochrome b(562); PQQ glucose dehydrogenase; glucose oxidase
MeSH headings : Acinetobacter / enzymology; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Coenzymes; Cytochrome b Group / chemistry; Electrochemistry / instrumentation; Electrochemistry / methods; Electron Transport; Enzymes, Immobilized / chemistry; Escherichia coli / enzymology; Escherichia coli Proteins / chemistry; Glucose / analysis; Glucose / chemistry; Glucose 1-Dehydrogenase / chemistry; Glucose Oxidase / chemistry; Multienzyme Complexes / chemistry; PQQ Cofactor / chemistry
TL;DR:
It is demonstrated that a sensor system can be constructed without a synthetic electron mediator by using a natural electron acceptor and the potential application of cyt b(562) in direct electron transfer type sensor systems with oxidoreductases whose quaternary structure do not contain any electron transfer subunit.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2003 journal article
Improved substrate specificity of water-soluble pyrroloquinoline quinone glucose dehydrogenase by a peptide ligand
Biotechnology Letters, 25(4), 301–305.
Contributors: H. Yoshida *, Y. Yagi*, K. Ikebukuro * & *
author keywords: peptide ligand; phage display; PQQ-glucose dehydrogenase; quaternary structure design; substrate specificity
MeSH headings : Amino Acid Sequence; Carbohydrates / chemistry; Carbohydrates / classification; Coenzymes / chemistry; Enzyme Activation; Glucose Dehydrogenases / chemistry; Ligands; Macromolecular Substances; Molecular Sequence Data; Peptide Library; Peptides / chemistry; Protein Engineering / methods; Protein Subunits / chemistry; Quality Control; Sequence Analysis, Protein / methods; Solubility; Substrate Specificity; Water / chemistry
TL;DR:
A new approach in altering the substrate specificity of enzyme is proposed using glucose dehydrogenase, with pyrroroquinoine quinone (PQQGDH) as co-factor, as the model, based on the selection of random peptide phage displayed library.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2003 journal article
Molecular imprinting catalyst based artificial enzyme sensor for fructosylamines
Analytical Letters, 36(1), 75–89.
Contributors: T. Yamazaki *, S. Ohta*, Y. Yanai* & *
TL;DR:
The molecularly imprinted catalyst was synthesized by imprinting polyvinylimidazole based polymer using fructosyl valine (Fru-val) that is the model compound for HbA1c as template molecules, based on both typical principles for molecular imprinting, noncovalent and covalent approaches.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2003 journal article
Relationship between serum N-carbamoyl-beta-D-glucopyranosylamine level and renal failure
Renal Failure, 25(1), 115–121.
Contributors: T. Hamafuji*, W. Tsugawa * & A. Sode *
author keywords: hemodialysis; N-carbamoyl-beta-D-glucopyranosylamine; glucose 3-dehydrogenasa
MeSH headings : Biomarkers / blood; Diabetes Mellitus / blood; Glucosamine / analogs & derivatives; Glucosamine / blood; Humans; Renal Dialysis; Renal Insufficiency / blood; Renal Insufficiency / therapy; Syndrome
TL;DR:
Results indicate that a high serum NCG level is a feature of renal failure patients, and a relationship was demonstrated between hyperuremia and NCG formation and accumulation in blood.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2003 journal article
Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase
Molecular Biotechnology, 24(2), 97–103.
author keywords: pyrroloquinoline quinone (PQQ); glucose dehydrogenase (GDH); thermal stability; dimmer interface; subunit-subunit interaction
MeSH headings : Computer Simulation; Dimerization; Disulfides / chemistry; Enzyme Activation; Enzyme Stability; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Macromolecular Substances; Models, Molecular; Mutation; Protein Conformation; Protein Engineering; Protein Structure, Quaternary; Protein Subunits; Recombinant Proteins / chemistry; Solubility; Structure-Activity Relationship; Temperature; Water / chemistry
TL;DR:
The results indicate that the introduction of one Cys residue in each monomer of PQQGDH-B resulted in formation of a disulfide bond at the dimer interface and thus achieved a large increase in the thermal stability of the enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2003 journal article
Surface charge engineering of PQQ glucose dehydrogenase for downstream processing
Biotechnology Letters, 25(20), 1695–1701.
author keywords: downstream process; poly-arginine tail (Arg-tail); pyrroloquinoline quinone glucose dehydrogenase; (PQQGDH); surface charge engineering
MeSH headings : Chromatography, Ion Exchange / methods; Enzyme Activation; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Genetic Enhancement / methods; Glucose Dehydrogenases / biosynthesis; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Glucose Dehydrogenases / isolation & purification; Mutagenesis, Site-Directed; Mutation; Protein Engineering / methods; Recombinant Fusion Proteins / biosynthesis; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / isolation & purification; Static Electricity; Substrate Specificity; Surface Properties
TL;DR:
The ion-exchange chromatography behavior of recombinant glucose dehydrogenase harboring pyrroloquinoline quinone was modified to greatly simplify its purification and resulted in enzymes with increased thermal stability.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2002 journal article
Amperometric DNA sensor using the pyrroquinoline quinone glucose dehydrogenase-avidin conjugate
Biosensors & Bioelectronics, 17(11-12), 1075–1080.
Contributors: K. Ikebukuro *, Y. Kohiki* & *
author keywords: pyrroquinoline quinone glucose dehydrogenase ((PQQ)GDH); DNA sensor; amperometry; (PQQ)GDH-avidin conjugate; Salmonella virulence invA gene; carbon paste electrode
MeSH headings : Avidin / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Biotin / chemistry; Calibration; Carbon; DNA / analysis; DNA / chemistry; DNA Probes / analysis; DNA, Bacterial / analysis; DNA, Bacterial / chemistry; Electrochemistry / instrumentation; Electrochemistry / methods; Electrodes; Enzymes, Immobilized / chemistry; Equipment Design; Glucose / analysis; Glucose / chemistry; Glucose Dehydrogenases / chemistry; Materials Testing / methods; Reference Values; Reproducibility of Results; Salmonella / genetics; Salmonella / metabolism; Sensitivity and Specificity
TL;DR:
A new amperometric DNA sensor was constructed using a pyrroquinoline quinone glucose dehydrogenase conjugated with avidin to specifically detect the DNA sequence of the invA virulence gene from the pathogenic bacterium Salmonella.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2002 journal article
Clinical application of the serum 1,5-anhydroglucitol assay method using glucose 3-dehydrogenase
Journal of Clinical Laboratory Analysis, 16(6), 299–303.
Contributors: T. Hamafuji*, W. Tsugawa * & *
author keywords: 1,5-anhydroglucitol; glucose 3-dehydrogenase; clinical analysis; validation
MeSH headings : Blood Chemical Analysis; Deoxyglucose / blood; Dose-Response Relationship, Drug; Evaluation Studies as Topic; Glucose Dehydrogenases / metabolism; Humans; Plasma / chemistry; Plasma / metabolism; Reproducibility of Results; Tetrazolium Salts / metabolism
TL;DR:
A novel serum 1,5‐anhydroglucitol assay kit using glucose 3‐dehydrogenase (G3DH) from Halomonas sp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2002 journal article
Construction of engineered water-soluble PQQ glucose dehydrogenase with improved substrate specificity
Biocatalysis and Biotransformation, 20(6), 405–412.
Contributors: * , S. Igarashi*, A. Morimoto* & H. Yoshida *
author keywords: pyrroloquinoline quinone (PQQ); glucose dehydrogenase; substrate specificity; protein engineering; glucose sensor; beta-propeller protein
TL;DR:
The predicted 3D structures of Asn452Thr and the wild-type enzyme indicated that the most significant impact of the amino acid substitution was observed in the interaction between the 6BC loop region with lactose.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2002 journal article
Development of a flow-injection analysis (FIA) enzyme sensor for fructosyl amine monitoring
Analytical and Bioanalytical Chemistry, 373(4-5), 211–214.
Contributors: K. Ogawa*, D. Stollner*, F. Scheller *, A. Warsinke*, F. Ishimura*, W. Tsugawa *, S. Ferri *, *
author keywords: enzyme sensor; glycated hemoglobin; fructosyl amine; fructosyl amine oxidase; FIA system
MeSH headings : Amino Acid Oxidoreductases; Biosensing Techniques / instrumentation; Biosensing Techniques / methods; Biosensing Techniques / standards; Calibration; Enzymes, Immobilized; Glycated Hemoglobin / analysis; Glycoproteins / analysis; Humans; Lysine / analogs & derivatives; Lysine / analysis; Valine / analogs & derivatives; Valine / analysis
TL;DR:
An enzyme-sensor system with flow-injection analysis (FIA) has been developed for the detection of fructosyl amine compounds; the sensor utilizes fructo-amine oxidase isolated from the marine yeast Pichia sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2002 journal article
Isolation and identification of N-carbamoyl-beta-D-glucopyranosylamine from human serum.
Journal of Biochemistry, Molecular Biology, and Biophysics : JBMBB : the Official Journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB), 6(5), 315–318.
MeSH headings : Carbohydrate Conformation; Chromatography, High Pressure Liquid; Glucosamine / analogs & derivatives; Glucosamine / blood; Glucosamine / chemistry; Glucosamine / isolation & purification; Humans; Models, Molecular
Source: ORCID
Added: October 9, 2020
2002 journal article
Multi-sugar analysis system using a novel glucose-3-dehydrogenase electrode
Instrumentation Science & Technology, 30(1), 97–105.
Contributors: T. Hamafuji*, Y. Takano*, W. Tsugawa * & *
UN Sustainable Development Goal Categories
10. Reduced Inequalities
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2002 journal article
Secretion of water soluble pyrroloquinoline quinone glucose dehydrogenase by recombinant Pichia pastoris
Enzyme and Microbial Technology, 30(3), 312–318.
Contributors: H. Yoshida *, N. Araki*, A. Tomisaka* & *
author keywords: PQQ glucose dehydrogenase; secretion; Pichia pastoris
TL;DR:
The production of water soluble pyrroloquinoline quinone glucose dehydrogenase from Acinetobacter calcoaceticus was carried out by using recombinant Pichia pastoris by using Saccharomyces cerevisiae α-factor signal sequence instead of the native signal sequence of PQQGDH-B.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2002 journal article
The application of cytochromes as the interface molecule to facilitate the electron transfer for PQQ glucose dehydrogenase employing mediator type glucose sensor
Analytical Letters, 35(9), 1465–1478.
TL;DR:
The future application of cytochromes as an essential component for the improvement of sensor response in the redox enzyme-based amperometric sensors is demonstrated.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2001 journal article
A new concept for the construction of an artificial dehydrogenase for fructosylamine compounds and its application for an amperometric fructosylamine sensor
Analytica Chimica Acta, 435(1), 151–156.
Contributors: * , Y. Takahashi*, S. Ohta*, W. Tsugawa * & T. Yamazaki *
Source: ORCID
Added: October 9, 2020
2001 journal article
A novel amperometric sensor for organophosphotriester insecticides detection employing catalytic polymer mimicking phosphotriesterase catalytic center
Electrochemistry, 69(12), 969–972. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000172742300016&KeyUID=WOS:000172742300016
Source: ORCID
Added: October 9, 2020
2001 journal article
Biodegradation of formaldehyde by a formaldehyde-resistant bacterium isolated from seawater
Applied Biochemistry and Biotechnology, 91-3, 213–217.
Contributors: T. Yamazaki *, W. Tsugawa * & *
author keywords: biodegradation; formaldehyde; marine bacterium; screening
MeSH headings : Bacteria / drug effects; Bacteria / isolation & purification; Bacteria / metabolism; Biodegradation, Environmental; Colony Count, Microbial; Culture Media; Drug Resistance, Bacterial; Formaldehyde / metabolism; Formaldehyde / pharmacology; Kinetics; Seawater / microbiology; Water Pollutants, Chemical / metabolism; Water Pollutants, Chemical / pharmacology
TL;DR:
A formaldehyde-tolerantbacterium designated as a DM-2 strain was used to biodegrade formaldehyde, and cells were able to degrade formaldehyde in a minimal medium supplemented with up to 400 ppm offormaldehyde in the presence of 3% NaCl.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2001 journal article
Cloning and expression of glucose 3-dehydrogenase from Halomonas sp alpha-15 in Escherichia coli
Biochemical and Biophysical Research Communications, 282(1), 21–27.
Contributors: K. Kojima *, W. Tsugawa * & *
author keywords: glucose 3-dehydrogenase; marine bacteria; Halomonas sp.
MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Bacterial; Escherichia coli / genetics; Genes, Bacterial; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Halomonas / enzymology; Molecular Sequence Data; Sequence Homology, Amino Acid
TL;DR:
The deduced primary structure of G3DH showed about 30% identity to sorbitol dehydrogenase from Gluconobacter oxydans and 2-keto- d -gluconate dehydrogenases from Erwinia herbicola and Pantoea citrea.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2001 journal article
Enzymatic synthesis of a novel trehalose derivative, 3,3 '-diketotrehalose, and its potential application as the trehalase enzyme inhibitor
Febs Letters, 489(1), 42–45.
Contributors: * , E. Akaike*, H. Sugiura* & W. Tsugawa *
author keywords: glucose-3-dehydrogenase; trehalose; trehalase; inhibitor; insecticide
MeSH headings : Animals; Binding, Competitive; Bombyx / enzymology; Catalysis; Enzyme Inhibitors / chemical synthesis; Enzyme Inhibitors / chemistry; Enzyme Inhibitors / pharmacology; Glucose 1-Dehydrogenase; Glucose Dehydrogenases / chemistry; Halomonas / enzymology; Kinetics; Trehalase / antagonists & inhibitors; Trehalose / analogs & derivatives; Trehalose / chemical synthesis; Trehalose / chemistry
TL;DR:
3,3′dkT was revealed to show an inhibitory effect toward pig‐kidney and Bombyx mori trehalase and was a specific inhibitor of trehalases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2001 journal article
Fructosyl amine sensing based on Prussian blue modified enzyme electrode
Electrochemistry, 69(12), 973–975. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000172742300017&KeyUID=WOS:000172742300017
Source: ORCID
Added: October 9, 2020
2001 journal article
Increasing the hydrophobic interaction between terminal W-motifs enhances the stability of Salmonella typhimurium sialidase. A general strategy for the stabilization of beta-propeller protein fold
Protein Engineering, 14(11), 891–896.
Contributors: A. Witarto * & *
author keywords: hydrophobic interaction; beta-propeller protein; sialidase; stabilization; terminal W-motifs
MeSH headings : Alanine / chemistry; Amino Acid Motifs; Circular Dichroism; Cloning, Molecular; Guanidine / pharmacology; Kinetics; Leucine / chemistry; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Neuraminidase / chemistry; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Salmonella typhimurium / enzymology; Thermodynamics
TL;DR:
Al Ala53Leu and Ala69 each located at strands B and C of the W1 motif were mutated to Leu and Val, respectively, to increase the hydrophobic interaction between W1 and W6 motifs, which showed enhanced stability towards guanidine hydrochloride and thermal unfolding.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2001 journal article
Screening and characterization of fructosyl-valine-utilizing marine microorganisms
Marine Biotechnology, 3(2), 126–132.
Contributors: * , F. Ishimura* & W. Tsugawa *
author keywords: fructosyl-valine; Amadori compounds; glycated hemoglobin (Hb A(1C)); diabetes; biosensors
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2001 journal article
Towards the use of molecularly imprinted polymers containing imidazoles and bivalent metal complexes for the detection and degradation of organophosphotriester pesticides
Analytica Chimica Acta, 435(1), 209–214.
Contributors: T. Yamazaki *, E. Yilmaz, K. Mosbach & *
author keywords: molecularly imprinted polymers; imidazoles; organophosphotriester pesticides
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2000 journal article
A Salmonella detection system using an engineered DNA binding protein that specifically captured a DNA sequence
Analytical Chemistry, 72(13), 2809–2813.
Contributors: A. Takeuchi * & *
MeSH headings : Culture Media; DNA Primers; DNA, Bacterial / chemistry; DNA-Binding Proteins / genetics; Genes, Bacterial / genetics; Reverse Transcriptase Polymerase Chain Reaction; Salmonella / chemistry
TL;DR:
A novel method for the detection with high selectivity of a double-stranded DNA fragment using an engineered DNA-binding protein, DnaA IV, a fusion protein of the DNA- binding domain of Dna a and glutathione S-transferase, which could distinguish Salmonella from 106 cfu/ mL contaminating bacteria.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2000 journal article
Construction and characterization of glucose enzyme sensor employing engineered water soluble PQQ glucose dehydrogenase with improved thermal stability
Electrochemistry, 68(11), 907–911. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000165420800021&KeyUID=WOS:000165420800021
Source: ORCID
Added: October 9, 2020
2000 journal article
Construction of multi-chimeric pyrroloquinoline quinone glucose dehydrogenase with improved enzymatic properties and application in glucose monitoring
Biotechnology Letters, 22(18), 1505–1510.
Contributors: H. Yoshida *, T. Iguchi* & *
author keywords: chimeric enzyme; dynamic range; glucose sensor; PQQ glucose dehydrogenase
TL;DR:
The application of the multi-chimeric PQQGDH in amperometric glucose sensor construction achieved an expanded dynamic range together with increased operational stability and narrower substrate specificity, suggesting its potential for the direct measurement of high blood-glucose levels in diabetic patients.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2000 journal article
Development of an enzyme sensor utilizing a novel fructosyl amine oxidase from a marine yeast
Electrochemistry, 68(11), 869–871. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000165420800009&KeyUID=WOS:000165420800009
Source: ORCID
Added: October 9, 2020
2000 journal article
Enzyme electrochemical preparation of a 3-keto derivative of 1,5-anhydro-D-glucitol using glucose-3-dehydrogenase
Applied Biochemistry and Biotechnology, 84-6, 947–954.
Contributors: * , H. Sugiura*, W. Tsugawa *, Y. Watazu & T. Hamafuji
author keywords: 1,5 anhydro-D-glucitol; glucose-3-dehydrogenase; electrochemistry; 3-keto sugars
MeSH headings : Biomarkers / blood; Deoxyglucose / blood; Diabetes Mellitus / blood; Diabetes Mellitus / diagnosis; Electrochemistry / methods; Glucose 1-Dehydrogenase; Glucose Dehydrogenases / isolation & purification; Glucose Dehydrogenases / metabolism; Halomonas / enzymology; Humans; Molecular Structure; Seawater / microbiology; Sorbitol / analogs & derivatives; Sorbitol / chemical synthesis; Sorbitol / chemistry
TL;DR:
A novel enzymatic organic synthesis was reported, utilizing glucose-3-dehydrogenase (G3DH) and its regeneration via electrochemical methods, utilizing water-soluble G3DH prepared from a marine bacterium and electron mediator with the electrode system in order to regenerate the enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2000 journal article
Extended-range glucose sensor employing engineered glucose dehydrogenases
Analytical Chemistry, 72(19), 4689–4693.
Contributors: T. Yamazaki *, K. Kojima * & *
MeSH headings : Biosensing Techniques; Colorimetry; Glucose / analysis; Glucose 1-Dehydrogenase; Glucose Dehydrogenases / metabolism
TL;DR:
The extended-range glucose sensor acquired not only the expanded dynamic range that covered both dynamic ranges of the single enzyme sensors but also the narrower substrate specificity of glucose due to the inherited property of engineered enzymes.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2000 journal article
Fluorescence resonance energy transfer from pyrene to perylene labels for nucleic acid hybridization assays under homogeneous solution conditions
Nucleic Acids Research, 28(8), 34.
MeSH headings : DNA, Bacterial / genetics; Escherichia coli / genetics; Fluoresceins / chemistry; Fluorescent Dyes / chemistry; Nucleic Acid Denaturation; Nucleic Acid Hybridization; Oligodeoxyribonucleotides / analysis; Oligodeoxyribonucleotides / chemistry; Oligonucleotide Probes; Perylene / chemistry; Pyrenes / chemistry; Rhodamines / chemistry; Solutions; Spectrometry, Fluorescence; Spectrophotometry; Temperature
TL;DR:
Comparison between the fluorescent properties of a pyrene-perylene FRET pair with those of a widely used fluorescein-rhodamine pair showed that the pyrene's FRET is suitable for hybridization assays.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2000 journal article
Improvement of enantioselectivity of chiral organophosphate insecticide hydrolysis by bacterial phosphotriesterase
Applied Biochemistry and Biotechnology, 84-6, 311–317.
Contributors: W. Tsugawa *, H. Nakamura *, * & S. Ohuchi*
author keywords: enantioselectivity; organophosphate insecticide; detoxification; phosphotriesterase
MeSH headings : Acetylcholinesterase / metabolism; Animals; Aryldialkylphosphatase; Biodegradation, Environmental; Chickens; Cholinesterase Inhibitors / pharmacology; Electrophorus; Esterases / metabolism; Female; Flavobacterium / enzymology; Houseflies; Insecticides / chemistry; Insecticides / metabolism; Insecticides / toxicity; Kinetics; Organophosphorus Compounds; Stereoisomerism; Substrate Specificity
TL;DR:
This work attempted to alter the enantioselectivity of PTE in order to degrade toxic EPN enantiomer effectively, and found that PTE preferred R-EPN over S-EPn in the presence of DMSO by a factor of 2.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2000 journal article
Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement
Enzyme and Microbial Technology, 26(7), 491–496.
Contributors: * , T. Ootera*, M. Shirahane*, A. Witarto *, S. Igarashi* & H. Yoshida
author keywords: pyrroloquinoline quinone; glucose dehydrogenase; biosensor; thermal stability
TL;DR:
Considering that Ser231Lys showed more than an 8-fold increase in its half-life during the thermal inactivation at 55 degrees C compared with the wild-type enzyme, the application of this mutant enzyme as a glucose sensor constituent may develop into a stable glucose sensor construction.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
2000 journal article
Novel approaches toward bioelectrochemical applications of genetically engineered proteins - 2. Engineering of substrate specificity of PQQ glucose dehydrogenase
Electrochemistry, 68(5), 348–352. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000087236200008&KeyUID=WOS:000087236200008
Source: ORCID
Added: October 9, 2020
2000 journal article
Surface deposition method; A novel protein immobilization procedure on a sensor surface
Electrochemistry, 68(11), 882–885. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000165420800013&KeyUID=WOS:000165420800013
Source: ORCID
Added: October 9, 2020
2000 journal article
The production of soluble pyrroloquinoline quinone glucose dehydrogenase by Klebsiella pneumoniae, the alternative host of PQQ enzymes
Biotechnology Letters, 22(16), 1343–1347.
Contributors: K. Kojima *, A. Witarto * & *
author keywords: glucose dehydrogenase; Klebsiella pneumoniae; pyrroloquinoline quinone; recombinant production
TL;DR:
The recombinant K. pneumoniaeexpressed PQQGDH-B in its holo-form at about 18 000 U l−1, equal to that achieved in recombinant Escherichia coli, and became an alternative host microorganism not only for PQZG DH-B production but also for recombinant P QQ enzymes production.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
2000 journal article
The role of conserved His775/781 in membrane-binding PQQ glucose dehydrogenase of Escherichia coli and Acinetobacter calcoaceticus
Journal of Biochemistry, Molecular Biology and Biophysics, 4(6), 415–422. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034524811&partnerID=MN8TOARS
Source: ORCID
Added: October 9, 2020
1999 journal article
Construction and characterization of a linked-dimeric pyrroloquinoline quinone glucose dehydrogenase
Biotechnology Letters, 21(8), 707–710.
Contributors: * , M. Shirahane* & H. Yoshida *
author keywords: biosensor; glucose dehydrogenase; protein engineering; pyrroloquinoline quinone
TL;DR:
Using the structural gene of a homo dimeric enzyme, the water-soluble pyrroloquinoline quinone glucose dehydrogenase (PQQGDH-B), a gene consisting of two identical subunits linked together by a DNA segment coding linker peptide region was constructed and produced in Escherichia coli as the active soluble enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1999 journal article
Construction and characterization of fermentative lactate dehydrogenase Escherichia coli mutant and its potential for bacterial hydrogen production
Applied Biochemistry and Biotechnology, 77-9, 317–323.
author keywords: metabolic engineering; lactate dehydrogenase; hydrogen; production; Escherichia coli
TL;DR:
In Escherichia coli, classified as a mixed-acid producer in fermentation, D-lactate is one of the final metabolites from pyruvate and the effect of uptake hydrogenases on the hydrogen production was discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1999 journal article
Construction and characterization of mutant water-soluble PQQ glucose dehydrogenases with altered K-m values - Site-directed mutagenesis studies on the putative active site
Biochemical and Biophysical Research Communications, 264(3), 820–824.
Contributors: S. Igarashi*, T. Ohtera*, H. Yoshida *, A. Witarto * & *
author keywords: pyrroloquinoline quinone (PQQ); glucose dehydrogenase; s-GDH; catalytic activity; biosensor
MeSH headings : Amino Acid Sequence; Amino Acid Substitution; Animals; Escherichia coli; Glucose Dehydrogenases / chemistry; Glucose Dehydrogenases / genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation
TL;DR:
Based on a PCR mutant enzyme of water-soluble glucose dehydrogenase-harboring pyrroloquinoline quinone as the prosthetic group, PQQGDH-B, a site-directed mutagenesis study was carried out and the substitution of Glu277 residue with Gly resulted in a decrease in the K(m) value for glucose and altered the substrate specificity profile, compared with the wild-type enzyme.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1999 journal article
Design of new primers that can detect Salmonella without decrease of detection threshold in the presence of contaminant organisms
Biotechnology Techniques, 13(1), 81–85.
author keywords: Salmonella; Citrobacter; PCR; primer; threshold
TL;DR:
Using the PCR primers, the selective detection of Salmonella in the presence of large excess number of salmonella-like strains were achieved without the reduction of its sensitivity.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1999 journal article
Effect of growth substrates on production of new soluble glucose 3-dehydrogenase in Halomonas (Deleya) sp alpha-15
Applied Biochemistry and Biotechnology, 77-9, 827–834. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000081133100076&KeyUID=WOS:000081133100076
Source: ORCID
Added: October 9, 2020
1999 journal article
Engineering a chimeric pyrroloquinoline quinone glucose dehydrogenase: improvement of EDTA tolerance, thermal stability and substrate specificity
Protein Engineering, 12(1), 63–70.
Contributors: H. Yoshida *, K. Kojima *, A. Witarto * & *
author keywords: PQQ glucose dehydrogenase; chimeric enzymes; biosensor; substrate specificity; thermal stability
MeSH headings : Acinetobacter / chemistry; Amino Acid Sequence; Circular Dichroism; Edetic Acid / pharmacology; Enzyme Stability; Escherichia coli / chemistry; Genotype; Glucose Dehydrogenases / chemistry; Kinetics; Models, Biological; Molecular Sequence Data; Phenotype; Protein Engineering; Protein Structure, Secondary; Recombinant Fusion Proteins; Sequence Homology, Amino Acid; Substrate Specificity; Temperature; Time Factors
TL;DR:
An engineered Escherichia coli PQQ glucose dehydrogenase (PQQGDH) with improved enzymatic characteristics was constructed by substituting and combining the gene-encoding protein regions responsible for EDTA tolerance, thermal stability and substrate specificity to suggest a strategy for the construction of a semi-artificial enzyme.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1999 journal article
Increased thermal stability of glucose dehydrogenase by cross-linking chemical modification
Biotechnology Letters, 21(3), 199–202.
Contributors: T. Yamazaki *, W. Tsugawa * & *
author keywords: thermal stabilization; glucose dehydrogenase; cross-linking chemical modification; amperometric glucose sensor; storage stability
TL;DR:
A hetero-oligomeric glucose dehydrogenase (GDH) from a moderate thermophilic bacterium, SM4 was cross-linked with glutaraldehyde (GA) and it now showed only one optimum temperature for reaction at around 65 °C, which approximately follows the Arrhenius equation.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1999 journal article
Secondary structure study of pyrroloquinoline quinone glucose dehydrogenase
Journal of Biochemistry, Molecular Biology and Biophysics, 2(3), 209–213. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032956367&partnerID=MN8TOARS
Source: ORCID
Added: October 9, 2020
1999 journal article
Single amino acid preferences for specific locations at type-I alpha-turns in globular proteins
Bulletin of the Chemical Society of Japan, 72(8), 1807–1813.
TL;DR:
Similarities in the structure and amino acid preferences for specific locations among them suggest that the amino acid residues on defined positions along both turns could be properly assigned to the type-I α-turns.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1999 journal article
Site-directed mutagenesis study on the thermal stability of a chimeric PQQ glucose dehydrogenase and its structural interpretation
Applied Biochemistry and Biotechnology, 77-9, 159–168.
Contributors: A. Witarto *, T. Ohtera* & *
author keywords: PQQ glucose dehydrogenase; beta-propeller protein; site-directed mutagenesis; CD spectroscopy; denaturation pathway
MeSH headings : Circular Dichroism; Glucose Dehydrogenases / chemistry; Models, Biological; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Recombinant Fusion Proteins / chemistry; Temperature
TL;DR:
Comparison of the thermal denaturation of E. coli PQQGDH and E97A3 followed by far-ultraviolet (UV) circular dichroism (CD) spectroscopy revealed that E97 A3 acquired stability at the first step of denaturation, which is reversible, and where no significant secondary structure change was observed.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1999 journal article
Subunit analyses of a novel thermostable glucose dehydrogenase showing different temperature properties according to its quaternary structure
Applied Biochemistry and Biotechnology, 77-9, 325–335.
Contributors: T. Yamazaki *, W. Tsugawa * & *
author keywords: glucose dehydrogenase; thermal stability; catalytic subunit; electron transfer subunit; quaternary structures
Source: ORCID
Added: October 9, 2020
1998 journal article
Construction of a marine cyanobacterial strain with increased heavy metal ion tolerance by in introducing exogenic metallothionein gene
Journal of Marine Biotechnology, 6(3), 174–177. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000075383800008&KeyUID=WOS:000075383800008
Source: ORCID
Added: October 9, 2020
1998 journal article
Effect of hydrogenase 3 over-expression and disruption of nitrate reductase on fermamentive hydrogen production in Escherichia coli - A metabolic engineering approach
Biohydrogen, 73–79. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000078629000009&KeyUID=WOS:000078629000009
Source: ORCID
Added: October 9, 2020
1998 journal article
Fluorescent measurement of 1,5-anhydro-D-glucitol based on a novel marine bacterial glucose dehydrogenase
Enzyme and Microbial Technology, 22(4), 269–274.
Contributors: W. Tsugawa *, N. Ogasawara* & *
author keywords: 1,5-anhydro-D-glucitol; diabetes; glucose dehydrogenase; marine bacteria
TL;DR:
A novel enzymatic method for 1-5AG determination was developed utilizing the fluorescent electron mediator, AlamarBlue tm, and a good linear correlation was observed from 2.5–200 μ m of 1,5AG, which covers both the healthy subjects' and the diabetics' range in blood.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1998 journal article
Statistical characterization of eleven kinds of helix elements with amino acid residues in the middle of triplets
Bulletin of the Chemical Society of Japan, 71(2), 385–395.
TL;DR:
The 681 helices were built up from 8836 amino acid residues, which could be classified into 11 kinds of helix elements (a—k), and Glycine residues in the proteins were used for its precise assignment as internal standards.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1997 journal article
A new type of phi,psi-representation of the protein tertiary structure and the analysis of the amino acid preferences for specific locations at type-II beta-turn by using 8000 possible kinds of amino acid residues
Bulletin of the Chemical Society of Japan, 70(7), 1639–1648.
TL;DR:
A new type of representation of the protein tertiary structure can be used as a stereotyped finger printing for 125 protein molecules for a precise comparison of the relationship of the primary, secondary, and tertiary structures of homologous proteins.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1997 journal article
Alteration of substrate specificity of galactose oxidase by chemical cross-linking
Denki Kagaku, 65(6), 435–439. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1997XG00300003&KeyUID=WOS:A1997XG00300003
Source: ORCID
Added: October 9, 2020
1997 journal article
An optical resolution of racemic organophosphorous esters by phosphotriesterase-catalyzing hydrolysis
Applied Biochemistry and Biotechnology, 63-5, 659–665.
Contributors: S. Ohuchi*, H. Nakamura *, H. Sugiura, M. Narita* & *
author keywords: phosphotriesterase; stereospecific reaction; organophosphorous insecticide; phosphotriesters; kinetic optical resolution
TL;DR:
It is shown that PTE chiral recognition is limited to organophosphorous esters having the chiral center on the phosphorous atoms, which is attacked with nucleophile, not having theChiral Center on the other atoms of the leaving group, therefore, PTE can be utilized for the synthesis of their chiral organoph phosphate esters.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1997 journal article
Biochemical properties and colony formation patterns of salmonella-like strains
Biocontrol Science, 2(2), 93–97.
Contributors: A. Takeuchi * & *
TL;DR:
Five bacterial strains were isolated from food samples, and were not easily distinguished from Salmonella via conventional methods, but their characteristics after being cultured resembled those ofSalmonella, with similar kinds of colony formation patterns on XLD, DHL and TSI agar.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1997 journal article
Compatibility of phosphotriesterase from Flavobacterium sp. with detergents
Biotechnology Letters, 19(12), 1239–1242.
Contributors: * & H. Nakamura *
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1997 journal article
Construction and characterization of a chimeric Escherichia coli PQQ glucose dehydrogenase (PQQGDH) with increased EDTA tolerance
Denki Kagaku, 65(6), 444–451. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1997XG00300005&KeyUID=WOS:A1997XG00300005
Source: ORCID
Added: October 9, 2020
1997 journal article
Growth properties of a marine bacterium capable of assimilating 1,5-anhydro-D-glucitol as the sole carbon source
Journal of Marine Biotechnology, 5(4), 201–204. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1997XR47000003&KeyUID=WOS:A1997XR47000003
Source: ORCID
Added: October 9, 2020
1997 journal article
Improved substrate specificity and dynamic range for glucose measurement of Escherichia coli PQQ glucose dehydrogenase by site directed mutagenesis
Biotechnology Letters, 19(11), 1073–1077.
Contributors: * & K. Kojima *
TL;DR:
H775D showed the increase in K value for glucose versus wild type PQQGDH (25-fold), therefore H775D is suitable for the direct measurement of blood glucose.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1997 journal article
Induction of a temperate marine cyanophage by heavy metal
Journal of Marine Biotechnology, 5(2-3), 178–180. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1997XJ35800021&KeyUID=WOS:A1997XJ35800021
Source: ORCID
Added: October 9, 2020
1997 journal article
Preparation of lyophilized pyrroloquinoline quinone glucose dehydrogenase using trehalose as an additive
Biotechnology Techniques, 11(8), 577–580.
TL;DR:
Of 8 compounds tested as additives, trehalose at 100 mM was the most effective for the preparation of lyophilized pyrroloquinoline quinone glucose dehydrogenase (PQQGDH), suitable for use as a biosensor component.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1996 journal article
A novel thermostable glucose dehydrogenase varying temperature properties by altering its quaternary structures
Enzyme and Microbial Technology, 19(2), 82–85.
Contributors: * , W. Tsugawa *, T. Yamazaki *, M. Watanabe *, N. Ogasawara* & M. Tanaka*
author keywords: thermostable enzyme; glucose dehydrogenase; thermophilic bacteria; enzyme quaternary structure
TL;DR:
This novel enzyme was purified from a Gram-negative moderate thermophilic bacterium isolated from soil near a hot spring and concluded that this novel enzyme showed a different optimum temperature for reaction according to its quaternary structure.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1996 journal article
A novel transesterification catalyzed by phosphotriesterase in an organic solvent
Biotechnology Letters, 18(8), 923–926.
Contributors: * , S. Ohuchi*, H. Nakamura * & M. Narita*
TL;DR:
Phosphotriesterase (PTE) from Flavobacterium sp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1996 journal article
Cloning of a marine cyanobacterial promoter for foreign gene expression using a promoter probe vector
Applied Biochemistry and Biotechnology, 59(3), 349–360.
MeSH headings : Base Sequence; Chloramphenicol O-Acetyltransferase / metabolism; Chromatography, High Pressure Liquid; Cloning, Molecular; Cyanobacteria / genetics; DNA Probes; Gene Expression Regulation, Bacterial / physiology; Genetic Vectors; Genome, Bacterial; Promoter Regions, Genetic; Spectrometry, Fluorescence
TL;DR:
C Cyanobacterial transformants, harboring plasmid containing a cloned 2-kbp marine cyanobacterial genomic fragment, showed a 10-fold higher CAT activity, compared with that achieved using the kanamycin-resistant gene promoter.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1996 journal article
Development of a novel conductometric determination of organophosphate insecticides using phosphotriesterase
Denki Kagaku, 64(12), 1234–1238. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1996VY99100005&KeyUID=WOS:A1996VY99100005
Source: ORCID
Added: October 9, 2020
1996 journal article
Increased production of recombinant pyrroloquinoline quinone (PQQ) glucose dehydrogenase by metabolically engineered Escherichia coli strain capable of PQQ biosynthesis
Journal of Biotechnology, 49(1-3), 239–243.
Contributors: * , K. Ito *, A. Witarto *, K. Watanabe*, H. Yoshida * & P. Postma*
author keywords: pyrroloquinoline quinone (PQQ); glucose dehydrogenase; metabolic engineering; Acinetobacter calcoaceticus
MeSH headings : Acinetobacter calcoaceticus / enzymology; Acinetobacter calcoaceticus / genetics; Enzyme Stability; Escherichia coli / genetics; Escherichia coli / metabolism; Glucose Dehydrogenases / biosynthesis; Glucose Dehydrogenases / genetics; PQQ Cofactor; Quinolones / metabolism; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics
TL;DR:
It is shown that the increase in the accumulated PQQGDH is due to the increased stability of the holo-enzyme over apo- enzyme, using recombinant Acinetobacter calcoaceticus PQZGDH, which allowed E. coli to produce P QQ.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1996 book
Marine Biotechnology Based on Marine Microorganisms
In Advances in Molecular and Cell Biology (Vol. 15, pp. 95–102).
TL;DR:
This review summarizes the recent research on marine biotechnology based on marine microorganisms and constructed gene manipulating systems as well as host-vector systems, and developed bioreactors and monitoring systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1996 journal article
Purification of a marine bacterial glucose dehydrogenase from Cytophaga marinoflava and its application for measurement of 1,5-anhydro-D-glucitol
Applied Biochemistry and Biotechnology, 56(3), 301–310.
Contributors: W. Tsugawa *, S. Horiuchi*, M. Tanaka*, H. Wake & *
author keywords: glucose dehydrogenase; marine bacteria; 1,5-anhydro-D-glucitol; diabetes; Cytophaga mauinoflava
MeSH headings : Chromatography / methods; Cytophaga / enzymology; Deoxyglucose / analysis; Glucose 1-Dehydrogenase; Glucose Dehydrogenases / isolation & purification
TL;DR:
A novel glucose dehydrogenase (GDH) from a marine bacterium Cytophaga marinoflava IFO 14170 was isolated from its membrane fraction and an enzymatic determination of 1,5-anhydro-D-glucitol (1,5AG) utilizing 2,6-dichrolophenolindophenol (DCIP) and phenazine methosulfate (PMS) as electron mediators was caried out.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1996 journal article
Recovery of a marine cyanobacterial recombinant product using fish-feed organisms
Journal of Marine Biotechnology, 4(2), 82–86. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI199799471382&KeyUID=BCI:BCI199799471382
Source: ORCID
Added: October 9, 2020
1996 journal article
Stabilization of pyrroloquinoline quinone glucose dehydrogenase by cross-linking chemical modification
Biotechnology Letters, 18(9), 997–1002.
Contributors: * , T. Shimakita*, S. Ohuchi* & T. Yamazaki *
TL;DR:
Cross-linking chemical modification of pyrroloquinoline quinone (PQQ) glucose dehydrogenase (GDH) by glutaraldehyde was carried out and its stability was analyzed and cross-linked PQQGDH gained high EDTA tolerance.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1995 journal article
A marine bacterial electrode for sensitive detection of 1,5-anhydro-D-glucitol
Denki Kagaku, 63(12), 1131–1133. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1995TK61200010&KeyUID=WOS:A1995TK61200010
Source: ORCID
Added: October 9, 2020
1995 journal article
An iron-regulated gene, magA, encoding an iron transport protein of Magnetospirillum sp. strain AMB-1
Journal of Biological Chemistry, 270(47), 28392–28396.
Contributors: C. Nakamura *, J. Burgess *, * & T. Matsunaga*
MeSH headings : Amino Acid Sequence; Bacteria / genetics; Bacteria / isolation & purification; Bacteria / metabolism; Bacterial Proteins / chemistry; Base Sequence; Blotting, Northern; Carrier Proteins / biosynthesis; Carrier Proteins / chemistry; Carrier Proteins / genetics; Carrier Proteins / metabolism; Cation Transport Proteins; Cloning, Molecular; DNA Transposable Elements; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial / drug effects; Iron / metabolism; Iron / pharmacology; Kinetics; Magnetics; Molecular Sequence Data; Mutagenesis, Insertional; Open Reading Frames; Potassium Channels; RNA, Messenger / analysis; RNA, Messenger / biosynthesis; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Restriction Mapping; Sequence Homology, Amino Acid
TL;DR:
It is considered that the MagA protein is an iron transport involved in the synthesis of magnetic particles in AMB-1, a freshwater magnetic bacterium which synthesizes intracellular particles of magnetite (FeO).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1995 journal article
Analysis of a gene, magA, required for magnetite biomineralization in the bacterium Magnetospirillum sp. AMB-1
Bulletin De l'Institut Oceanographique (Monaco), 0(SPEC. ISSUE 14 PART 2), 121–126. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI199698775342&KeyUID=BCI:BCI199698775342
Source: ORCID
Added: October 9, 2020
1995 journal article
CONTINUOUS 2-STEP BIOCONVERSION OF 4-OXOISOPHORONE (OIP) TO 4-HYDROXY-2,2,6-TRIMETHYLCYCLOHEXANONE (4-HTMCH) BY THERMOPHILIC BACTERIA
Biotechnology Letters, 17(8), 785–790.
TL;DR:
The continuous two step conversion of 4-oxoisophorone to 4-hydroxy-2,2,6-trimethylcyclohexanone(4-HTMCH) via dihydrooxoisPhorone (DOIP) was carried out using two types of thermophilic growing cells in sequential connected continuous stirred tank reactor (CSTR) and hollow fiber reactor system.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1995 journal article
Determination of the metal binding region of pyrroloquinoline quinone glucose dehydrogenase
Protein Engineering, 8(9), 51. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1995TL57300062&KeyUID=WOS:A1995TL57300062
Source: ORCID
Added: October 9, 2020
1995 journal article
EFFECT OF PQQ GLUCOSE-DEHYDROGENASE OVEREXPRESSION IN ESCHERICHIA-COLI ON SUGAR-DEPENDENT RESPIRATION
Journal of Biotechnology, 43(1), 41–44.
Contributors: * , S. Sugimoto*, M. Watanabe * & W. Tsugawa *
author keywords: PYRROLOQUINOLINE QUINONE; GLUCOSE DEHYDROGENASE; RESPIRATION; METABOLIC ENGINEERING; MICROBIAL SENSOR
TL;DR:
It is suggested that PQQGDH overexpression may alter sugar utilization preferences in E. coli, suggesting further possible applications in metabolic engineering for carbon source utilization.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1995 journal article
ELUCIDATION OF THE REGION RESPONSIBLE FOR EDTA TOLERANCE IN PQQ GLUCOSE DEHYDROGENASES BY CONSTRUCTING ESCHERICHIA-COLI AND ACINETOBACTER-CALCOACETICUS CHIMERIC ENZYMES
Biochemical and Biophysical Research Communications, 211(1), 268–273.
MeSH headings : Acinetobacter calcoaceticus / enzymology; Amino Acid Sequence; Base Sequence; DNA Primers; Drug Tolerance; Edetic Acid / pharmacology; Escherichia coli / enzymology; Glucose 1-Dehydrogenase; Glucose Dehydrogenases / antagonists & inhibitors; Glucose Dehydrogenases / biosynthesis; Glucose Dehydrogenases / metabolism; Isoenzymes / antagonists & inhibitors; Isoenzymes / biosynthesis; Isoenzymes / metabolism; Molecular Sequence Data; PQQ Cofactor; Plasmids; Quinolones / analysis; Recombinant Fusion Proteins / antagonists & inhibitors; Recombinant Fusion Proteins / biosynthesis; Recombinant Fusion Proteins / metabolism; Restriction Mapping; Sequence Homology, Amino Acid
TL;DR:
It is demonstrated that EDTA tolerance of PQQGDHs can be completely altered by substituting each corresponding region by homologous recombination of their structural genes.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1995 journal article
Preference of a specific primary structure in the helix of proteins and its regiospecificity
Protein Engineering, 8(9), 48. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1995TL57300059&KeyUID=WOS:A1995TL57300059
Source: ORCID
Added: October 9, 2020
1995 journal article
SCREENING OF MARINE CYANOBACTERIA FOR HIGH PALMITOLEIC ACID PRODUCTION
Fems Microbiology Letters, 133(1-2), 137–141.
Contributors: T. Matsunaga*, H. Takeyama *, Y. Miura *, T. Yamazaki *, H. Furuya & *
author keywords: MARINE CYANOBACTERIA; PHORMIDIUM SP; FATTY ACID; PALMITOLEIC ACID
TL;DR:
Results indicate that this cyanobacterium could be considered as a new source for palmitoleic acid.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1995 journal article
SCREENING OF MELANIN BIOSYNTHESIS INHIBITORS FROM MARINE MICROALGAE USING STREPTOMYCES-BIKINIENSIS BIOASSAY
Biotechnology Techniques, 9(9), 633–636.
Contributors: Y. Wachi*, * , K. Horikoshi*, H. Takeyama * & T. Matsunaga*
TL;DR:
Inhibitors of melanin biosynthesis from marine microalgae were screened against a melanin-producing microorganism, Streptomyces bikiniensis, and extracts from two marine green algae showed strongly inhibited melan in biosynthesis, but showed less than 30 % inhibitory activity against mushroom tyrosinase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1995 journal article
Screening of marine cyanobacteria for high palmitoleic acid production
FEMS Microbiology Letters, 133(1-2), 137–141.
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1995 journal article
THERMOSTABLE CHIMERIC PQQ GLUCOSE-DEHYDROGENASE
Febs Letters, 364(3), 325–327.
Contributors: * , K. Watanabe*, S. Ito *, K. Matsumura * & T. Kikuchi*
author keywords: PQQ (PYRROLOQUINOLINE QUINONE); GLUCOSE DEHYDROGENASE; CHIMERIC ENZYME; THERMAL STABILITY; PROTEIN ENGINEERING
MeSH headings : Acinetobacter calcoaceticus / enzymology; Amino Acid Sequence; Enzyme Activation; Enzyme Stability; Escherichia coli / enzymology; Glucose 1-Dehydrogenase; Glucose Dehydrogenases / chemistry; Hot Temperature; Kinetics; Molecular Sequence Data; Recombinant Fusion Proteins / chemistry; Thermolysin
TL;DR:
Using transition state theory, the increase in the free energy of inactivation observed in E97A3 was compared with those of the E. coli and A. calcoaceticus parental enzymes and showed a more than 3‐fold and 12‐fold increase in half life time at 40°C.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1994 journal article
CONSTRUCTION AND CHARACTERIZATION OF CHIMERIC QUINOPROTEIN GLUCOSE-DEHYDROGENASE BETWEEN ESCHERICHIA-COLI AND ACINETOBACTER-CALCOACETICUS
Protein Engineering, 7(9), 1154. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1994PG50700021&KeyUID=WOS:A1994PG50700021
Source: ORCID
Added: October 9, 2020
1994 chapter
ENZYME SENSOR FOR MEASUREMENT OF 1,5-ANHYDRO-D-GLUCITOL UTILIZING A NOVEL MARINE BACTERIAL GLUCOSE DEHYDROGENASE
In Advanced Materials '93, Ii - a & B (Vol. 15, pp. 445–447). http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1994BC69F00099&KeyUID=WOS:A1994BC69F00099
Source: ORCID
Added: October 9, 2020
1994 journal article
FOREIGN GENE-EXPRESSION IN MARINE CYANOBACTERIA UNDER PSEUDOCONTINUOUS CULTURE
Journal of Biotechnology, 33(3), 243–248.
author keywords: MARINE CYANOBACTERIA; FOREIGN GENE EXPRESSION; CHLORAMPHENICOL ACETYL-TRANSFERASE; PSEUDOCONTINUOUS CULTURE; BROAD HOST RANGE VECTOR
MeSH headings : Chloramphenicol O-Acetyltransferase / biosynthesis; Chloramphenicol O-Acetyltransferase / genetics; Cyanobacteria / genetics; Cyanobacteria / metabolism; Recombinant Proteins / biosynthesis
TL;DR:
Foreign gene expression in a marine cyanobacterium, Synechococcus NKBG 15041c, has been carried out in both batch and pseudo-continuous culture, using chloramphenicaol acetyltransferase (CAT) as a model peptide and the broad host range vector pKT230 to express CAT.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1994 journal article
GLU742 SUBSTITUTION TO LYS ENHANCES THE EDTA TOLERANCE OF ESCHERICHIA-COLI PQQ GLUCOSE-DEHYDROGENASE
Biotechnology Letters, 16(5), 455–460.
Contributors: * & H. Sano *
author keywords: PYRROLOQUINOLINE QUINONE(PQQ); QUINOPROTEIN GLUCOSE DEHYDROGENASE (GDH); PUTATIVE PQQ BINDING SITE; SITE DIRECTED MUTAGENESIS; ESCHERICHIA COLI; ACINETOBACTER CALCOACETICUS
TL;DR:
This is the first mutagenesis study of putative a P QQ binding site in PQQ enzyme and the mutant enzyme, E742K showed higher tolerance towards EDTA inactivation than wild type PQZGDH.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1994 journal article
Over expression of PQQ glucose dehydrogenase in Escherichia coli under holo enzyme forming condition
Biotechnology Letters, 16(12), 1265–1268.
Contributors: * , A. Witarto *, K. Watanabe*, K. Noda*, S. Ito * & W. Tsugawa *
TL;DR:
The level of P QQGDH expressed in E.coli PP2418/pGEc1 was more than 10 fold when the cultivation was carried out under holo enzyme forming condition in the presence of 600 nM of PQQ and 10 mM of MgCl2, compared with those of apo condition.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1994 journal article
PSEUDOCONTINUOUS CULTURE OF MARINE RECOMBINANT CYANOBACTERIA UNDER A LIGHT-DARK CYCLE
Biotechnology Letters, 16(9), 973–976.
TL;DR:
Pseudo-continuous culture of recombinant marine cyanobacteria under a light dark cycle indicates the possibility of regulation of foreign gene expression by controlling light illumination and indicates the lack of energy for foreign peptide synthesis during dark incubation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1994 journal article
SELECTIVE DETECTION OF MESSENGER-RNA BY OLIGO-DNA IMMOBILIZED BACTERIAL MAGNETITE
Advanced Materials '93, Ii - a & B, 15(A -B), 449–454. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1994BC69F00100&KeyUID=WOS:A1994BC69F00100
Source: ORCID
Added: October 9, 2020
1993 journal article
APPLICATION OF BACTERIAL MAGNETIC PARTICLES FOR HIGHLY SELECTIVE MESSENGER-RNA RECOVERY-SYSTEM
Biotechnology Techniques, 7(9), 688–694.
Contributors: * , S. Kudo*, T. Sakaguchi*, N. Nakamura * & T. Matsunaga*
TL;DR:
A novel mRNA detection system was developed using DNA immobilized on bacterial magnetic particles isolated from the magnetic bacterium Magnetospirillum sp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1993 journal article
CHARACTERIZATION OF IRON UPTAKE IN THE MAGNETIC BACTERIUM AQUASPIRILLUM SP AMB-1
Applied Biochemistry and Biotechnology, 39(1), 169–176.
Contributors: C. Nakamura *, T. Sakaguchi*, S. Kudo*, J. Burgess *, * & T. Matsunaga*
author keywords: BIOMINERALIZATION; MAGNETITE; AQUASPIRILLUM; IRON UPTAKE; NUCLEOTIDE BINDING PROTEIN
TL;DR:
The results suggest that a nucleotide binding protein mediated iron transport system similar to that observed inSerratia marcescens is present in Aquaspirillum sp.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1993 journal article
CONSTRUCTION AND CHARACTERIZATION OF LOW-TEMPERATURE-OPERATING BIOSENSORS
Sensors and Actuators B-Chemical, 14(1-3), 663–664.
Contributors: * , S. Nakasono *, M. Tanaka* & T. Matsunaga*
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1993 journal article
DEVELOPMENT OF RAPID DETECTION SYSTEM FOR ALLERGIC REACTION USING RAT BASOPHILIC LEUKEMIA (RBL-1) CELLS
Sensors and Actuators B-Chemical, 13(1-3), 312–314.
Contributors: N. Nakamura *, S. Kumazawa*, * & T. Matsunaga*
TL;DR:
The electrochemical detection system of allergic reaction using rat basophilic leukemia (RBL-1) cells by applying cyclic voltammetry finds that this current increase is attributed to localization of the intracellular serotonin and is specific for allergen.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1993 journal article
Docosahexaenoic acid production by the unicellular alga Isochrysis galbana using a photobioreactor
Japanese Journal of Phycology, 41(4), 414. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI199497157951&KeyUID=BCI:BCI199497157951
Source: ORCID
Added: October 9, 2020
1993 journal article
EFFICIENT GENE-TRANSFER OF HIGH COPY NUMBER MOBILIZABLE PLASMIDS CONTAINING A MARINE RHODOBACTER ORIGIN OF REPLICATION
Current Microbiology, 26(2), 105–108.
Contributors: J. Burgess *, H. Sudo*, * & T. Matsunaga*
Source: ORCID
Added: October 9, 2020
1993 journal article
Light-induced antimicrobial activity of extracts from marine .
Journal of Marine Biotechnology, 1(3), 143–146. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=CABI&KeyUT=CABI:19941301477&KeyUID=CABI:19941301477
Source: ORCID
Added: October 9, 2020
1993 journal article
PRODUCTION OF GAMMA-LINOLENIC ACID FROM THE MARINE GREEN-ALGA CHLORELLA SP NKG-042401
Fems Microbiology Letters, 107(2-3), 163–167.
Contributors: Y. Miura *, * , N. Nakamura *, N. Matsunaga * & T. Matsunaga*
author keywords: CHLORELLA SP; GREEN ALGAE; FATTY ACID; GAMMA-LINOLENIC ACID (GLA); ENVIRONMENT
MeSH headings : Chlorella / drug effects; Chlorella / growth & development; Chlorella / metabolism; Fatty Acids / biosynthesis; Linolenic Acids / biosynthesis; Linolenic Acids / isolation & purification; Nitrogen / pharmacology; Osmolar Concentration; Sodium Chloride; gamma-Linolenic Acid
TL;DR:
γ-Linolenic acid (GLA) production using a high GLA producing marine green alga, Chlorella sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1993 journal article
SUBZERO TEMPERATURE OPERATING BIOSENSOR UTILIZING AN ORGANIC-SOLVENT AND QUINOPROTEIN GLUCOSE-DEHYDROGENASE
Biotechnology and Bioengineering, 42(2), 251–254.
Contributors: * , S. Nakasono *, M. Tanaka* & T. Matsunaga*
author keywords: BIOSENSOR; SUBZERO TEMPERATURE; PQQGDH
TL;DR:
Using this biosensor, glucose concentration can be determined even at -7 degrees C, and the response was 20% of that obtained at 20 degrees C.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1992 journal article
A NOVEL MICROBIAL SENSOR USING LUMINOUS BACTERIA
Biosensors & Bioelectronics, 7(4), 273–277.
Contributors: S. Lee*, * , K. Nakanishi*, J. Marty *, E. Tamiya * & I. Karube*
author keywords: PHOTOMICROBIAL SENSOR; LUMINOUS BACTERIA; FLOW INJECTION ANALYSIS; GLUCOSE MEASUREMENT; TOXIC COMPOUND MEASUREMENT
MeSH headings : Bacteria; Benzalkonium Compounds / analysis; Biosensing Techniques; Chromium / analysis; Glucose / analysis; Luminescent Measurements; Sodium Dodecyl Sulfate / analysis
TL;DR:
A novel microbial sensor system that uses luminous bacteria was developed for the determination of both glucose and toxic compounds and a linear relationship was observed between increased luminescence and concentrations of glucose between 0.05 mM and 0.55 mM.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1992 journal article
BIOSENSOR FOR DETECTION OF ORGANOPHOSPHATE AND CARBAMATE INSECTICIDES
Electroanalysis, 4(2), 249–252.
Contributors: J. Marty *, * & I. Karube*
TL;DR:
An enzyme sensor for the determination of organophosphate and carbamate insecticides is described, based on the coimmobilization and sequential enzymatic reactions of acetylcholinesterase and choline oxidase.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1992 journal article
CO2 REMOVAL BY HIGH-DENSITY CULTURE OF A MARINE CYANOBACTERIUM SYNECHOCOCCUS SP USING AN IMPROVED PHOTOBIOREACTOR EMPLOYING LIGHT-DIFFUSING OPTICAL FIBERS
Applied Biochemistry and Biotechnology, 34-5(1), 449–458.
Contributors: H. Takano*, H. Takeyama *, N. Nakamura *, * , J. Burgess *, E. Manabe*, M. Hirano*, T. Matsunaga*
author keywords: PHOTOBIOREACTOR; MARINE CYANOBACTERIUM; SYNECHOCOCCUS; CO2 REMOVAL; OPTICAL FIBER
TL;DR:
Results demonstrate the potential of LDOF photobioreactor units for CO2 removal and biomass production using marine cyanobacteria.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1992 journal article
Conjugative gene transfer in marine cyanobacteria: Synechococcus sp., Synechocystis sp. and Pseudanabaena sp.
Applied Microbiology and Biotechnology, 37(3), 369–373.
Contributors: * , M. Tatara*, H. Takeyama *, J. Burgess * & T. Matsunaga*
MeSH headings : Biotechnology; Conjugation, Genetic; Cyanobacteria / genetics; Genetic Engineering; Genetic Vectors; Plasmids; Species Specificity
TL;DR:
This is the first study to demonstrate gene transfer in marine cyanobacteria via transconjugation using the mobilizable transposon (Tn5) carrying plasmid pSUP1021, and the broad-host-range vector pKT230 (IncQ) was tested for trans Conjugation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1992 journal article
DISINFECTION OF DRINKING-WATER BY USING A NOVEL ELECTROCHEMICAL REACTOR EMPLOYING CARBON-CLOTH ELECTRODES
Applied and Environmental Microbiology, 58(2), 686–689. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1992HC49400037&KeyUID=WOS:A1992HC49400037
Source: ORCID
Added: October 9, 2020
1992 journal article
ELECTROCHEMICAL DISINFECTION OF MARINE-BACTERIA ATTACHED ON A PLASTIC ELECTRODE
Bioelectrochemistry and Bioenergetics, 27(2), 191–198.
Contributors: S. Nakasono *, N. Nakamura *, * & T. Matsunaga*
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1992 journal article
Electrochemical disinfection of marine bacteria attached on a plastic electrode
Journal of Electroanalytical Chemistry, 342(2), 191–198.
Contributors: S. Nakasono *, N. Nakamura *, * & T. Matsunaga*
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1992 journal article
Gene transfer in magnetic bacteria: Transposon mutagenesis and cloning of genomic DNA fragments required for magnetosome synthesis
Journal of Bacteriology, 174(9), 2748–2753.
Contributors: T. Matsunaga*, C. Nakamura *, J. Burgess * & *
MeSH headings : Cloning, Molecular; Conjugation, Genetic; DNA Transposable Elements; Escherichia coli / genetics; Ferrosoferric Oxide; Genetic Vectors; Iron / metabolism; Mutagenesis, Insertional; Oxides / metabolism; Plasmids / genetics; Restriction Mapping; Spirillum / genetics; Transfection
TL;DR:
Broad-host-range IncP and IncQ plasmids have been transferred to the aerobic magnetic bacterium Aquaspirillum sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1992 journal article
INFLUENCE OF AMMONIUM-CHLORIDE ON GROWTH AND FATTY-ACID PRODUCTION BY SPIRULINA-PLATENSIS
Applied Biochemistry and Biotechnology, 34-5(1), 273–281.
author keywords: SPIRULINA-PLATENSIS; BLUE-GREEN ALGA; FATTY ACID; GAMMA-LINOLENIC ACID; AMMONIUM CHLORIDE
TL;DR:
Changes in growth and fatty acid content of Spirulina platensis were examined after transferring cells into media containing various concentrations of ammonium chloride and γ-linolenic acid content increased continuously during a 72-h incubation.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1992 journal article
MAINTENANCE OF BROAD HOST RANGE VECTOR PKT230 IN MARINE UNICELLULAR CYANOBACTERIA
Fems Microbiology Letters, 99(1), 73–78.
TL;DR:
The host range of pKT230 in marine unicellular cyanobacteria was investigated and p KT230 stability in Synechococcus sp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1991 journal article
Application of Novel Sensors for Bioprocess On-Line Monitoring to Dextran Fermentation
KAGAKU KOGAKU RONBUNSHU, 17(3), 559–564.
Contributors: H. Endo *, M. Suzuki *, * , E. Tamiya * & I. Karube*
author keywords: ONLINE MONITORING; BIOPROCESS; DEXTRAN; SENSOR; CELL CONCENTRATION; VISCOSITY; SUCROSE
TL;DR:
This paper focuses on mesenteroides, a new approach to cell reprograming that combines 3D image analysis and 3D lieutenants.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1991 journal article
DEVELOPMENT OF ACETYLCHOLINE SENSOR USING CARBON-FIBER (AMPEROMETRIC DETERMINATION)
Biosensors & Bioelectronics, 6(8), 675–680.
Contributors: E. Navera*, * , E. Tamiya * & I. Karube*
author keywords: CARBON FIBER; CO-IMMOBILIZED ENZYMES; PVA-SBQ; AMPEROMETRIC DETECTION
MeSH headings : Acetylcholine / analysis; Ascorbic Acid / analysis; Biosensing Techniques; Calibration; Carbon / chemistry; Electrochemistry; Electrodes; Enzyme Stability; Enzymes, Immobilized; Hydrogen-Ion Concentration; Temperature
TL;DR:
The use of carbon fiber containing co-immobilized enzymes could offer several novel advantages especially in neuroscience research and the aims of the present work are centered on carbon fiber electrode fabrication, immobilization and electrochemical measurements.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1991 journal article
GLUTAMATE PRODUCTION FROM CO2 BY MARINE CYANOBACTERIUM SYNECHOCOCCUS SP USING A NOVEL BIOSOLAR REACTOR EMPLOYING LIGHT-DIFFUSING OPTICAL FIBERS
Applied Biochemistry and Biotechnology, 28-9(1), 157–167.
Contributors: T. Matsunaga*, H. Takeyama *, H. Sudo*, N. Oyama*, S. Ariura*, H. Takano, M. Hirano, J. Burgess *, * , N. Nakamura *
author keywords: GLUTAMATE; MARINE CYANOBACTERIA; SYNECHOCOCCUS; BIOSOLAR REACTOR; OPTICAL FIBER
TL;DR:
It is concluded that as a result of its high surface-to-volume ratio (692/m) increased photoproduction of useful compounds may be achieved and is generally applicable to all aspects of photobiotechnology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1991 journal article
ONLINE MONITORING OF MARINE CYANOBACTERIAL CULTIVATION BASED ON PHYCOCYANIN FLUORESCENCE
Journal of Biotechnology, 21(3), 209–217.
Contributors: * , K. Horikoshi*, H. Takeyama *, N. Nakamura * & T. Matsunaga*
author keywords: ONLINE FLUORESCENCE MONITORING; MARINE CYANOBACTERIA; PHYCOCYANIN; SYNECHOCOCCUS SP
MeSH headings : Biotechnology / methods; Colony Count, Microbial; Cyanobacteria / growth & development; Cyanobacteria / metabolism; Flow Cytometry; Fluorescence; Kinetics; Phycocyanin / metabolism; Spectrometry, Fluorescence / instrumentation
TL;DR:
It is concluded that on-line monitoring of cyanobacterial culture fluorescence based on phycocyanin is a rapid, efficient and also versatile method for determining viable cell concentration.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1991 journal article
Preparation of Bacterial Magnetite Particles for DNA Carriers
KOBUNSHI RONBUNSHU, 48(5), 319–325.
Contributors: H. Takeyama *, S. Kudo*, * , M. Nakamura* & T. Matsunaga*
author keywords: MAGNETIC BACTERIUM; AQUASPIRILLUM; BACTERIAL MAGNETITE; DNA CARRIER; DNA ADSORPTION
Source: ORCID
Added: October 9, 2020
1991 journal article
RAPID-DETERMINATION OF PLASMID-CARRYING YEAST-CELLS BY USING AN IMAGING SENSOR SYSTEM
Biotechnology and Bioengineering, 38(11), 1331–1336.
Contributors: H. Endo *, M. Suzuki *, * , E. Tamiya * & I. Karube*
author keywords: PLASMID; YEAST; DETECTION; SENSOR; IMAGE ANALYSIS; 5-FLUORO-OROTIC ACID; DETERMINATION
TL;DR:
A novel imaging sensor system based on the fact that the membrane integrity of only plasmid‐carrying cells is lost following cell growth in 5‐fluoro‐orotic acid (5‐FOA) containing medium was developed, and consequently these target cell can be stained with fluorescent probes and detected.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1991 journal article
SALINITY-DEPENDENT COPY NUMBER INCREASE OF A MARINE CYANOBACTERIAL ENDOGENOUS PLASMID
Fems Microbiology Letters, 90(1), 95–98.
Contributors: H. Takeyama *, J. Burgess *, H. Sudo*, * & T. Matsunaga*
TL;DR:
This strain possesses a 2.7-kb endogenous plasmid pSY10, whose copy number increased five-fold when the salinity of the growth medium was increased from 0 to 3% NaCl, the first report of a cyanobacterial plasmids with a salinity-dependent copy number.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1991 journal article
Salinity-dependent copy number increase of a marine cyanobacterial endogenous plasmid
FEMS Microbiology Letters, 90(1), 95–98.
Contributors: H. Takeyama *, J. Grant Burgess, H. Sudo, & T. Matsunaga
author keywords: MARINE CYANOBACTERIUM; SYNECHOCOCCUS; COPY NUMBER; PLASMID; SODIUM CHLORIDE
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1991 book
WHOLE-ORGANISM BASED BIOSENSORS AND MICROBIOSENSORS
In Turner, A. P. F. (Ed.). Advances in Biosensors, Vol. 1. Xii+299p. Jai Press Ltd.: London, England, Uk; Greenwich, Connecticut, Usa. Illus (pp. 1–32). http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI199242103193&KeyUID=BCI:BCI199242103193
Source: ORCID
Added: October 9, 2020
1990 journal article
AIR-BUBBLING, HOLLOW-FIBER REACTOR WITH CELL BLEEDING AND CROSS-FLOW FILTRATION
Biotechnology and Bioengineering, 35(11), 1155–1160.
TL;DR:
Continuous asymmetric reduction of dyhydrooxoisophorone (DOIP) to 4‐hydroxy‐2,2,6‐trimethylcyclo‐hexanone (4‐HTMCH) was achieved by a thermophilic bacterium Bacillus stearothermophilus NK86‐0151 and a cell free product solution was continuously obtained by cross‐flow filtration.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1990 journal article
AMPEROMETRIC DETERMINATION OF CHOLINE AND ACETYLCHOLINE WITH ENZYMES IMMOBILIZED IN A PHOTOCROSS-LINKABLE POLYMER
Analytica Chimica Acta, 228(1), 49–53.
Contributors: J. Marty *, * & I. Karube*
TL;DR:
Choline and acetylcholine sensor membranes stored in a dry state were very stable and no loss of activity was observed after storage for 60 days.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1990 book
BIOSENSORS A DECADE OF RESEARCH IN JAPAN
In Finn, R. K. and P. Praeve (Ed.). Biotechnology Focus, Vol. 2. Fundamentals, Applications, Information. Viii+543p. Oxford University Press: Cary, North Carolina, Usa; Oxford, England, Uk; Hanser Publishers: Munich, West Germany. Illus (pp. 175–198). http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI199039072021&KeyUID=BCI:BCI199039072021
Source: ORCID
Added: October 9, 2020
1990 journal article
FACILE ISOLATION OF ENDO-PECTATE LYASE FROM ERWINIA-CAROTOVORA BASED ON ELECTROSTATIC INTERACTION
Applied Biochemistry and Biotechnology, 26(3), 239–248.
MeSH headings : Acetylation; Chlorides / pharmacology; Dialysis; Electrochemistry; Electrophoresis, Polyacrylamide Gel; Erwinia / enzymology; Hydrogen-Ion Concentration; Lipid Metabolism; Lipids / isolation & purification; Lipopolysaccharides / isolation & purification; Lipopolysaccharides / metabolism; Macromolecular Substances; Osmolar Concentration; Polymyxin B / pharmacology; Polysaccharide-Lyases / isolation & purification
TL;DR:
It was confirmed that the cosedimentation of PATE with LPSLC was induced by electrostatic interaction and was remarkably affected by pH or ionic strength.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1990 journal article
IDENTIFICATION OF PROTEINS ENCODED IN ESCHERICHIA-COLI HYDA, HYDB AND ANALYSIS OF THE HYDA LOCUS
Fems Microbiology Letters, 66(1-3), 187–192.
Contributors: T. Yamamoto *, M. Tomiyama*, H. Mita, * & I. Karube
Source: ORCID
Added: October 9, 2020
1990 journal article
Identification of proteins encoded in Escherichia coli hydA, hydB and analysis of the hydA locus
FEMS Microbiology Letters, 66(1-3), 187–192.
Contributors: T. Yamamoto *, M. Tomiyama, H. Mita, & I. Karube
MeSH headings : Bacterial Proteins / genetics; Cloning, Molecular; Escherichia coli / enzymology; Escherichia coli / genetics; Genes, Bacterial; Hydrogenase / genetics; Molecular Weight; Plasmids; Restriction Mapping
TL;DR:
The hydB gene of Escherichia coli, which is related with the expression of hydrogenase activity, was cloned into the plasmid (pES1) using the maxicell protein-labeling method and the molecular weight of the gene product was determined bymaxicell analysis.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1990 journal article
ONLINE MONITORING OF THE VISCOSITY IN DEXTRAN FERMENTATION USING PIEZOELECTRIC QUARTZ CRYSTAL
Biotechnology and Bioengineering, 36(6), 636–641.
Contributors: H. Endo *, * , I. Karube* & H. Muramatsu *
TL;DR:
A novel viscous sensor utilizing AT‐cut quartz crystal to monitor the viscosity of fermentation broth was developed and little bubbling effect and agitation of the sensor signal were observed, showing that this system can be utilized for Viscosity monitoring in a bioprocess.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1990 journal article
Restoration of escherichia coli hyd b mutant hydrogenase by cultivation in media containing various metals
Biocatalysis and Biotransformation, 4(2-3), 211–217.
Contributors: * , T. Sugiyama *, T. Yamamoto *, M. Tomiyama* & I. Karube*
TL;DR:
The leniency of HK7 hyd B product might permit the transportation and incorporation of iron chloride or vanadium chloride in hydrogenase, resulting in the alteration of hydrogenase activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1990 journal article
Sequential two-step conversion of 4-oxoisophorone to 4-hydroxy-2,2,6-trimethylcyclohexanone by thermophilic bacteria
Applied Microbiology and Biotechnology, 33(3), 245–250.
TL;DR:
The two-step conversion by a sequential culture system using T. curvata and B. stearothermophilus was found to be suitable for 4-HTMCH production and the total conversion yield from OIP was 83% through the two- step conversion.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1989 journal article
AMPEROMETRIC DETERMINATION OF FREE FATTY-ACIDS BY UTILIZING 5 SEQUENTIAL ENZYME-REACTIONS
Electroanalysis, 1(1), 69–74.
Contributors: * , E. Tamiya *, I. Karube* & Y. Kameda
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1989 journal article
BIOSENSOR FOR TOXIC COMPOUNDS USING IMMOBILIZED ANIMAL CELL MEMBRANE
Membrane, 14(5), 311–318. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI199089044064&KeyUID=BCI:BCI199089044064
Source: ORCID
Added: October 9, 2020
1989 journal article
Biosensor for toxic compounds using immobilized animal cell membrane
Membrane, 14(5), 311–318.
Source: ORCID
Added: October 9, 2020
1989 journal article
DETERMINATION OF MICROBIAL CONCENTRATION WITH A PIEZOELECTRIC GUM SENSOR
Journal of Biotechnology, 12(3-4), 307–316.
Contributors: H. Endo *, * , K. Ogura*, K. Ohya* & I. Karube*
TL;DR:
A novel piezoelectric gum sensor was developed for the determination of microbial concentration based on the attenuation of the sound intensity level in the presence of the microorganism, and a linear relationship between the output voltage and the cell concentration was obtained.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1989 journal article
EXTRACELLULAR LIPOPOLYSACCHARIDE PRODUCTION BY ERWINIA-CAROTOVORA
Journal of Fermentation and Bioengineering, 68(5), 320–324.
TL;DR:
The plant-pathogenic bacterium Erwinia carotovora FERM P-7576 produced lipopolysaccharide (LPS) in culture broth through a combination of two carbon sources, such as pectin and other carbohydrates, which promoted LPS production.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1989 journal article
INTEGRATED BIOSENSOR FOR GLUCOSE AND GALACTOSE
Analytica Chimica Acta, 218(1), 137–142.
Contributors: K. Yokoyama *, * , E. Tamiya * & I. Karube*
TL;DR:
A glucose microsensor based on quinoprotein glucose dehydrogenase (GDH) and electron mediators is described, which is unaffected by the concentration of dissolved oxygen and has insensitivity to oxygen.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1989 journal article
MICROBIAL CONVERSION OF BETA-IONONE BY IMMOBILIZED ASPERGILLUS-NIGER IN THE PRESENCE OF AN ORGANIC-SOLVENT
Biotechnology and Bioengineering, 33(9), 1191–1195.
TL;DR:
Aspergillus niger JTS191 was capable of the conversion of β‐ionone to a mixture of its derivatives that is utilized to an essential oil of tobacco and the addition of isooctane accelerated the microbial conversion and improved the resistance of A. niger to the antifungal property of β-ionone.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1989 journal article
MICROBIAL CONVERSION OF DIHYDROOXOISOPHORONE (DOIP) TO 4-HYDROXY-2,2,6-TRIMETHYLCYCLOHEXANONE (4-HTMCH) BY THERMOPHILIC BACTERIA
Journal of Biotechnology, 9(2), 117–128.
TL;DR:
The relationship between conversion of DOIP and cell growth was also investigated and it was found that this microbial conversion was closely associated with cell growth.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1989 journal article
MICROBIAL SENSOR FOR PRELIMINARY SCREENING OF MUTAGENS UTILIZING A PHAGE INDUCTION TEST
Analytical Chemistry, 61(21), 2388–2391.
MeSH headings : Bacteriophages / genetics; Biosensing Techniques; Escherichia coli / genetics; Mutagenicity Tests; Mutagens / analysis
Source: ORCID
Added: October 9, 2020
1989 journal article
Microbial bod sensor utilizing thermophilic bacteria
Analytical Letters, 22(4), 791–801.
Contributors: I. Karube*, * , E. Tamiya * & K. Yokoyama *
Source: ORCID
Added: October 9, 2020
1989 journal article
SENSOR FOR FREE FATTY-ACIDS BASED ON ACYL COENZYME-A SYNTHETASE AND ACYL COENZYME-A OXIDASE
Analytica Chimica Acta, 220(1), 251–255.
Contributors: * , E. Tamiya *, I. Karube* & Y. Kameda
Source: ORCID
Added: October 9, 2020
1988 journal article
APPLICATION OF MICROBIOLOGICAL SENSORS IN FERMENTATION PROCESSES
Analytica Chimica Acta, 213(1-2), 69–77.
Contributors: I. Karube*, E. Tamiya *, * , K. Yokoyama *, Y. Kitagawa*, H. Suzuki *, Y. Asano
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: October 9, 2020
1988 journal article
CONTINUOUS HYDROGEN EVOLUTION BY IMMOBILIZED RECOMBINANT ESCHERICHIA-COLI USING A BIOREACTOR
Biotechnology and Bioengineering, 32(3), 396–399.
TL;DR:
Etude de l'influence du taux de dilution sur la production d'hydrogene par fermentation du glucose en presence d' Escherichia coli ou d'EscherichIA coli C-600 ou de Citrobacter freundii TIT0101.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1988 journal article
CONTINUOUS PRODUCTION OF ALPHA-PEPTIDE USING IMMOBILIZED RECOMBINANT YEAST-CELLS - A MODEL FOR CONTINUOUS PRODUCTION OF FOREIGN PEPTIDE BY RECOMBINANT YEAST
Journal of Biotechnology, 8(2), 113–122.
Contributors: * , T. Morita *, A. Peterhans*, F. Meussdoerffer*, K. Mosbach* & I. Karube*
TL;DR:
The continuous production of α-peptide was performed by using immobilized recombinant yeast cells on a column reactor, after characterizing the secretion, using minimal and complex medium, and improvements were attempted by alternating minimal andcomplex medium.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1988 journal article
CONTINUOUS PRODUCTION OF SOMATOMEDIN-C WITH IMMOBILIZED TRANSFORMED YEAST-CELLS
Applied Microbiology and Biotechnology, 28(3), 215–221. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1988N050900001&KeyUID=WOS:A1988N050900001
Source: ORCID
Added: October 9, 2020
1988 journal article
EFFECT OF THE GLUCOSIDASE INHIBITOR 1-DEOXYNOJIRIMYCIN ON PROTEIN SECRETION FROM SACCHAROMYCES-CEREVISIAE
Enzyme and Microbial Technology, 10(4), 246–251.
Contributors: U. Greber *, * & F. Meussdoerffer*
TL;DR:
Deoxynojirimycin (dNM), an inhibitor of the trimming enzymes glucosidase I and II, increased secretion of a cloned human peptide hormone, somatomedin C, about fourfold, although the stimulation was reversible and directly dependent on dNM concentration.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1988 book
ENZYME AND MICROBIAL SENSOR
In Guilbault, G. G. and M. Mascini (Ed.). Nato Asi (Advanced Science Institute) Series Series C Mathematical and Physical Sciences, Vol. 226. Analytical Uses of Immobilized Biological Compounds For Detection, Medical and Industrial Uses; Workshop, Florence, Italy, May 4-8, 1987. Xv+396p. Kluwer Academic Publishers: Dordrecht, Netherlands; Boston, Massachusetts, Usa. Illus (pp. 115–130). http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI199038016591&KeyUID=BCI:BCI199038016591
Source: ORCID
Added: October 9, 2020
1988 book
INTEGRATED MICROBIOSENSORS FOR MEDICAL USE
In Blanch, H. W. and A. M. Klibanov (Ed.). Annals of the New York Academy of Sciences, Vol. 542. Enzyme Engineering; Ninth International Conference, Santa Barbara, California, USA, October 4-9, 1987. Xiv+535p. New York Academy of Sciences: New York, New York, Usa. Illus (pp. 470–479). http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI198937060611&KeyUID=BCI:BCI198937060611
Source: ORCID
Added: October 9, 2020
1988 journal article
Integrated microbiosensors for medical use.
Annals of the New York Academy of Sciences, 542, 470–479.
Contributors: I. Karube*, E. Tamiya *, * , K. Yokoyama *, H. Suzuki * & S. Hattori
MeSH headings : Biomedical Engineering / instrumentation; Biosensing Techniques; Enzymes, Immobilized / metabolism; Glucose / analysis; Glucose Oxidase / metabolism; Microchemistry; Oxygen
TL;DR:
Dans le but de developper des micro-analyseurs de taux de glucose in vivo, une electrode a oxygene avec une glucose oxydase immobilisee est construite, permettant la condensation du microdoseur en un appareil fiable pouvant.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1988 journal article
MICROBIAL CONVERSION OF 4-OXOISOPHORONE - BY THERMOMONOSPORA-CURVATA USING AN AIR-BUBBLING HOLLOW FIBER REACTOR
Applied Biochemistry and Biotechnology, 19(2), 209–220.
TL;DR:
In a continuous microbial conversion of OIP using a continuous stirred tank reactor, the cell density in the reactor seemed to be the limiting factor in the OIP conversion, so an air-bubbling hollow fiber reactor was developed to achieve a high density culture and more than 3.3 times higher productivity was achieved.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1988 book
MOLECULAR BREEDING OF HYDROGEN-EVOLVING BACTERIA
In Russell, G. E. (Ed.). Biotechnology and Genetic Engineering Reviews, Vol. 6. X+457p. Intercept Ltd.: Andover, England, Uk. Illus (pp. 379–402). http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=BCI&KeyUT=BCI:BCI199038054119&KeyUID=BCI:BCI199038054119
Source: ORCID
Added: October 9, 2020
1987 journal article
BASIC STUDIES OF HYDROGEN EVOLUTION BY ESCHERICHIA-COLI CONTAINING A CLONED CITROBACTER-FREUNDII HYDROGENASE GENE
Applied Biochemistry and Biotechnology, 15(2), 97–106.
MeSH headings : Citrobacter / enzymology; Citrobacter / genetics; Cloning, Molecular; DNA Restriction Enzymes; Escherichia coli / enzymology; Escherichia coli / genetics; Genes; Genes, Bacterial; Hydrogen / metabolism; Hydrogenase / genetics; Hydrogenase / metabolism; Kinetics; Plasmids
TL;DR:
Citrobacter freundii genes that complementedEscherichia coli hyd(hydrogenase activity) mutation were cloned in plasmids p CBH4 and pCBH6, and this high hydrogen evolution rate was maintained for more than 1 mo.
(via Semantic Scholar)
Source: ORCID
Added: October 9, 2020
1987 journal article
BIOSENSORS FOR LIPIDS
Fett Wissenschaft Technologie-Fat Science Technology, 89(12), 471. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1987L597000041&KeyUID=WOS:A1987L597000041
Source: ORCID
Added: October 9, 2020
Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.
Certain data included herein are derived from the Web of Science© and InCites© (2024) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.