@article{pereira_martins_batista-silva_condori-apfata_silva_oliveira_andrade_martins_medeiros_nascimento_et al._2024, title={Differential content of leaf and fruit pigment in tomatoes culminate in a complex metabolic reprogramming without growth impacts}, url={http://dx.doi.org/10.1016/j.jplph.2024.154170}, DOI={10.1016/j.jplph.2024.154170}, abstractNote={Although significant efforts to produce carotenoid-enriched foods either by biotechnology or traditional breeding strategies have been carried out, our understanding of how changes in the carotenoid biosynthesis might affect overall plant performance remains limited. Here, we investigate how the metabolic machinery of well characterized tomato carotenoid mutant plants [namely crimson (old gold-og), Delta carotene (Del) and tangerine (t)] adjusts itself to varying carotenoid biosynthesis and whether these adjustments are supported by a reprogramming of photosynthetic and central metabolism in the source organs (leaves). We observed that mutations og, Del and t did not greatly affect vegetative growth, leaf anatomy and gas exchange parameters. However, an exquisite metabolic reprogramming was recorded on the leaves, with an increase in levels of amino acids and reduction of organic acids. Taken together, our results show that despite minor impacts on growth and gas exchange, carbon flux is extensively affected, leading to adjustments in tomato leaves metabolism to support changes in carotenoid biosynthesis on fruits (sinks). We discuss these data in the context of our current understanding of metabolic adjustments and carotenoid biosynthesis as well as regarding to improving human nutrition.}, journal={Journal of Plant Physiology}, author={Pereira, Auderlan M. and Martins, Auxiliadora O. and Batista-Silva, William and Condori-Apfata, Jorge A. and Silva, Victor F. and Oliveira, Leonardo A. and Andrade, Eduarda Santos and Martins, Samuel C.V. and Medeiros, David B. and Nascimento, Vitor L. and et al.}, year={2024}, month={Jan} }
 @article{andrade_cardoso_oliveira_pereira_haverroth_souza_damatta_zsogon_martins_2024, title={Enhanced drought resistance in tomato via reduced auxin sensitivity: delayed dehydration and improved leaf resistance to embolism}, volume={176}, ISSN={["1399-3054"]}, url={https://doi.org/10.1111/ppl.14408}, DOI={10.1111/ppl.14408}, abstractNote={Abstract Auxins are master regulators of plant development and auxin perception mutants display smaller leaves, lower transpiration, and narrower xylem vessels than their corresponding wild types. Here, we evaluated whether the leaf embolism resistance and overall plant resistance to drought are altered in the auxin perception mutant diageotropica ( dgt ). Our assessments demonstrate that the dgt mutants in tomato exhibit considerably smaller xylem vessels in stems (‐24%), leaf petioles (‐43%), and leaf midribs (‐34%) than the wild type. Alongside narrower vessels, dgt mutants exhibited greater xylem cell wall thickness‐to‐conduit diameter and greater leaf embolism resistance than the wild type. The water potential at 50% cumulative leaf embolism (P 50 ) of dgt and wild type was ‐1.39 and ‐1.14 MPa, respectively. Plants of dgt also exhibited higher stomatal safety margin (water potential difference between stomatal closure and P 50 ), needed a longer time to reach their P 50 in a dry‐down experiment, and showed a faster recovery in leaf gas exchange upon rehydration than the wild type. The impaired auxin signaling resulted in lower canopy area and stomatal conductance, which likely contributed to delaying the time for plants to reach hydraulic damage during drought. These findings demonstrate a clear association between structural and physiological changes and improved resistance against drought‐induced hydraulic dysfunction in the dgt tomato mutant.}, number={3}, journal={PHYSIOLOGIA PLANTARUM}, author={Andrade, Moab T. and Cardoso, Amanda A. and Oliveira, Leonardo A. and Pereira, Talitha S. and Haverroth, Eduardo J. and Souza, Genaina A. and Damatta, Fabio M. and Zsogon, Agustin and Martins, Samuel C. V.}, year={2024}, month={May} }
 @article{haverroth_rimer_oliveira_lima_cesarino_martins_mcadam_cardoso_2024, title={Gradients in embolism resistance within stems driven by secondary growth in herbs}, volume={4}, ISSN={["1365-3040"]}, url={http://dx.doi.org/10.1111/pce.14921}, DOI={10.1111/pce.14921}, abstractNote={Abstract The stems of some herbaceous species can undergo basal secondary growth, leading to a continuum in the degree of woodiness along the stem. Whether the formation of secondary growth in the stem base results in differences in embolism resistance between the base and the upper portions of stems is unknown. We assessed the embolism resistance of leaves and the basal and upper portions of stems simultaneously within the same individuals of two divergent herbaceous species that undergo secondary growth in the mature stem bases. The species were Solanum lycopersicum (tomato) and Senecio minimus (fireweed). Basal stem in mature plants of both species displayed advanced secondary growth and greater resistance to embolism than the upper stem. This also resulted in significant vulnerability segmentation between the basal stem and the leaves in both species. Greater embolism resistance in the woodier stem base was found alongside decreases in the pith‐to‐xylem ratio, increases in the proportion of secondary xylem, and increases in lignin content. We show that there can be considerable variation in embolism resistance across the stem in herbs and that this variation is linked to the degree of secondary growth present. A gradient in embolism resistance across the stem in herbaceous plants could be an adaptation to ensure reproduction or basal resprouting during episodes of drought late in the lifecycle.}, journal={PLANT CELL AND ENVIRONMENT}, author={Haverroth, Eduardo J. and Rimer, Ian M. and Oliveira, Leonardo A. and Lima, Leydson G. A. and Cesarino, Igor and Martins, Samuel C. V. and Mcadam, Scott A. M. and Cardoso, Amanda A.}, year={2024}, month={Apr} }
 @article{pereira_oliveira_andrade_haverroth_cardoso_martins_2024, title={Linking water-use strategies with drought resistance across herbaceous crops}, volume={176}, ISSN={["1399-3054"]}, url={https://doi.org/10.1111/ppl.14114}, DOI={10.1111/ppl.14114}, abstractNote={AbstractWoody plants minimize xylem embolism formation during drought essentially by closing stomata at higher water potentials and/or by increasing the xylem resistance to embolism. Both of these mechanisms result in a higher stomatal safety margin (SSM), which is the water potential difference between stomatal closure and embolism formation. Here, we investigated whether increasing SSM represents a drought resistance mechanism for herbaceous plants and how the different water‐use strategies impact their survival. For that, we exposed four herbaceous crops with contrasting drought resistance to severe water deficit to assess drought‐induced damage and mortality. Unlike woody species, SSM was not associated with plant survival for herbaceous crops. Soybean, which presented the largest SSM across the four crops (1.67 MPa), exhibited the earliest mortality of leaves and whole plants as well as the highest rate of plant mortality (100%) at the end of the drought period. Cowpea, with an SSM of 0.63 MPa, was the most drought‐resistant species, with the latest leaf damage and the highest plant survival (100%). The most effective traits ensuring survival in herbaceous crops under severe drought were those related to drought avoidance mechanisms such as (1) early stomatal closure, (2) very low residual transpiration post‐stomatal closure, and (3) high capacitance pre‐ and post‐turgor loss.}, number={1}, journal={PHYSIOLOGIA PLANTARUM}, author={Pereira, Talitha S. and Oliveira, Leonardo A. and Andrade, Moab T. and Haverroth, Eduardo J. and Cardoso, Amanda A. and Martins, Samuel C. V.}, year={2024}, month={Jan} }
 @article{haverroth_da-silva_taggart_oliveira_cardoso_2024, title={Shoot hydraulic impairments induced by root waterlogging: Parallels and contrasts with drought}, volume={6}, ISSN={["1532-2548"]}, url={https://doi.org/10.1093/plphys/kiae336}, DOI={10.1093/plphys/kiae336}, abstractNote={Abstract Soil waterlogging and drought correspond to contrasting water extremes resulting in plant dehydration. Dehydration in response to waterlogging occurs due to impairments to root water transport, but no previous study has addressed whether limitations to water transport occur beyond this organ or whether dehydration alone can explain shoot impairments. Using common bean (Phaseolus vulgaris) as a model species, we report that waterlogging also impairs water transport in leaves and stems. During the very first hours of waterlogging, leaves transiently dehydrated to water potentials close to the turgor loss point, possibly driving rapid stomatal closure and partially explaining the decline in leaf hydraulic conductance. The initial decline in leaf hydraulic conductance (occurring within 24 h), however, surpassed the levels predicted to occur based solely on dehydration. Constraints to leaf water transport resulted in a hydraulic disconnection between leaves and stems, furthering leaf dehydration during waterlogging and after soil drainage. As leaves dehydrated later during waterlogging, leaf embolism initiated and extensive embolism levels amplified leaf damage. The hydraulic disconnection between leaves and stems prevented stem water potentials from declining below the threshold for critical embolism levels in response to waterlogging. This allowed plants to survive waterlogging and soil drainage. In summary, leaf and stem dehydration are central in defining plant impairments in response to waterlogging, thus creating similarities between waterlogging and drought. Yet, our findings point to the existence of additional players (likely chemicals) partially controlling the early declines in leaf hydraulic conductance and contributing to leaf damage during waterlogging.}, journal={PLANT PHYSIOLOGY}, author={Haverroth, Eduardo J. and Da-Silva, Cristiane J. and Taggart, Matthew and Oliveira, Leonardo A. and Cardoso, Amanda A.}, year={2024}, month={Jun} }
 @article{haverroth_oliveira_andrade_taggart_mcadam_zsogon_thompson_martins_cardoso_2023, title={Abscisic acid acts essentially on stomata, not on the xylem, to improve drought resistance in tomato}, volume={8}, ISSN={["1365-3040"]}, url={https://doi.org/10.1111/pce.14676}, DOI={10.1111/pce.14676}, abstractNote={AbstractDrought resistance is essential for plant production under water‐limiting environments. Abscisic acid (ABA) plays a critical role in stomata but its impact on hydraulic function beyond the stomata is far less studied. We selected genotypes differing in their ability to accumulate ABA to investigate its role in drought‐induced dysfunction. All genotypes exhibited similar leaf and stem embolism resistance regardless of differences in ABA levels. Their leaf hydraulic resistance was also similar. Differences were only observed between the two extreme genotypes: sitiens (sit; a strong ABA‐deficient mutant) and sp12 (a transgenic line that constitutively overaccumulates ABA), where the water potential inducing 50% embolism was 0.25 MPa lower in sp12 than in sit. Maximum stomatal and minimum leaf conductances were considerably lower in plants with higher ABA (wild type [WT] and sp12) than in ABA‐deficient mutants. Variations in gas exchange across genotypes were associated with ABA levels and differences in stomatal density and size. The lower water loss in plants with higher ABA meant that lethal water potentials associated with embolism occurred later during drought in sp12 plants, followed by WT, and then by the ABA‐deficient mutants. Therefore, the primary pathway by which ABA enhances drought resistance is via declines in water loss, which delays dehydration and hydraulic dysfunction.}, journal={PLANT CELL AND ENVIRONMENT}, publisher={Wiley}, author={Haverroth, Eduardo J. and Oliveira, Leonardo A. and Andrade, Moab T. and Taggart, Matthew and McAdam, Scott A. M. and Zsogon, Agustin and Thompson, Andrew J. and Martins, Samuel C. V. and Cardoso, Amanda A.}, year={2023}, month={Aug} }
 @article{oliveira_souza_andrade_oliveira_gouvea_martins_ramalho_cardoso_damatta_2023, title={Carbon gain is coordinated with enhanced stomatal conductance and hydraulic architecture in coffee plants acclimated to elevated [CO<sub>2</sub>]: The interplay with irradiance supply}, volume={204}, ISSN={["1873-2690"]}, url={http://dx.doi.org/10.1016/j.plaphy.2023.108145}, DOI={10.1016/j.plaphy.2023.108145}, abstractNote={We recently demonstrated that, under elevated [CO2] (eCa), coffee (Coffea arabica L.) plants grown at high light (HL), but not at low light (LL), display higher stomatal conductance (gs) than at ambient [CO2] (aCa). We then hypothesized that the enhanced gs at eCa/HL, if sustained at the long-term, would lead to adjustments in hydraulic architecture. To test this hypothesis, potted plants of coffee were grown in open-top chambers for 12 months under HL or LL (ca. 9 or 1 mol photons m-2 day-1, respectively); these light treatments were combined with two [CO2] levels (ca. 437 or 705 μmol mol-1, respectively). Under eCa/HL, increased gs was closely accompanied by increases in branch and leaf hydraulic conductances, suggesting a coordinated response between liquid- and vapor-phase water flows throughout the plant. Still under HL, eCa also resulted in increased Huber value (sapwood area-to-total leaf area), sapwood area-to-stem diameter, and root mass-to-total leaf area, thus further improving the water supply to the leaves. Our results demonstrate that Ca is a central player in coffee physiology increasing carbon gain through a close association between stomatal function and an improved hydraulic architecture under HL conditions.}, journal={PLANT PHYSIOLOGY AND BIOCHEMISTRY}, publisher={Elsevier BV}, author={Oliveira, Ueliton S. and Souza, Antonio H. and Andrade, Moab T. and Oliveira, Leonardo A. and Gouvea, Debora G. and Martins, Samuel C. V. and Ramalho, Jose D. C. and Cardoso, Amanda A. and DaMatta, Fabio M.}, year={2023}, month={Nov} }
 @article{souza_oliveira_oliveira_carvalho_andrade_pereira_gomes_cardoso_ramalho_martins_et al._2023, title={Growth and Leaf Gas Exchange Upregulation by Elevated [CO2] Is Light Dependent in Coffee Plants}, volume={12}, ISSN={["2223-7747"]}, url={https://doi.org/10.3390/plants12071479}, DOI={10.3390/plants12071479}, abstractNote={Coffee (Coffea arabica L.) plants have been assorted as highly suitable to growth at elevated [CO2] (eCa), although such suitability is hypothesized to decrease under severe shade. We herein examined how the combination of eCa and contrasting irradiance affects growth and photosynthetic performance. Coffee plants were grown in open-top chambers under relatively high light (HL) or low light (LL) (9 or 1 mol photons m−2 day−1, respectively), and aCa or eCa (437 or 705 μmol mol–1, respectively). Most traits were affected by light and CO2, and by their interaction. Relative to aCa, our main findings were (i) a greater stomatal conductance (gs) (only at HL) with decreased diffusive limitations to photosynthesis, (ii) greater gs during HL-to-LL transitions, whereas gs was unresponsive to the LL-to-HL transitions irrespective of [CO2], (iii) greater leaf nitrogen pools (only at HL) and higher photosynthetic nitrogen-use efficiency irrespective of light, (iv) lack of photosynthetic acclimation, and (v) greater biomass partitioning to roots and earlier branching. In summary, eCa improved plant growth and photosynthetic performance. Our novel and timely findings suggest that coffee plants are highly suited for a changing climate characterized by a progressive elevation of [CO2], especially if the light is nonlimiting.}, number={7}, journal={PLANTS-BASEL}, publisher={MDPI AG}, author={Souza, Antonio H. and Oliveira, Ueliton S. and Oliveira, Leonardo A. and Carvalho, Pablo H. N. and Andrade, Moab T. and Pereira, Talitha S. and Gomes, Carlos C. and Cardoso, Amanda A. and Ramalho, Jose D. C. and Martins, Samuel C. V. and et al.}, year={2023}, month={Apr} }
 @article{caetano-madeira_garcia_elerati_silva lopes_corrêa_souza_oliveira_cruz_bhering_nunes-nesi_et al._2023, title={Metabolic, Nutritional and Morphophysiological Behavior of Eucalypt Genotypes Differing in Dieback Resistance in Field When Submitted to PEG-Induced Water Deficit}, url={http://dx.doi.org/10.3390/agronomy13051261}, DOI={10.3390/agronomy13051261}, abstractNote={Dieback is a physiological disorder that has caused losses on eucalyptus plantations. Thinking that water stress is one of the triggers for the physiological disorder and aiming at the early identification of tolerant genotypes, we evaluated plantlets of four commercial clones with divergent behavior in field conditions. The plantlets were grown in a greenhouse where the drought conditions were provided by the application of polyethylene glycol 6000 solutions at 100 and 300 g L−1. After water deficit treatments, the morphological, physiological, nutritional and metabolic analyses were performed. SuzT maintained the carbon fixation and the instantaneous water use efficiency, even under water deficit, while sustaining growth. This resulted in higher leaf area and total dry mass in SuzT. Despite higher photosynthetic rate, SuzS exhibited reduced dry biomass accumulation, implying less efficient carbon use. SuzT revealed a higher level of calcium that supports cell structure and homeostasis and indicates higher capacity to manage specific resources and survival under water deficit. SuzT suffered reduction in some free amino acids; however, there was no significant variation for total amino acid content. The principal component and cluster analyses indicated that SuzMT and SuzTP genotypes behave similarly to SuzT under water deficit, while SuzS clustered in isolation. Our results support that there are common trends in water deficit responses for contrasting eucalypt genotypes. The existence of other strategies coping with water deficit resistance is not discarded and should be further evaluated.}, journal={Agronomy}, author={Caetano-Madeira, Débora Durso and Garcia, Rebeca Patrícia Omena and Elerati, Tamiris Lopes and Silva Lopes, Camila Batista and Corrêa, Thais Roseli and Souza, Genaína Aparecida and Oliveira, Leonardo Araujo and Cruz, Cosme Damião and Bhering, Leonardo Lopes and Nunes-Nesi, Adriano and et al.}, year={2023}, month={Apr} }
 @article{freitas_oliveira_mcadam_lawson_damatta_cardoso_2023, title={Woody species grown under sun and shade present similar stomatal speed}, volume={7}, ISSN={["2197-0025"]}, url={http://dx.doi.org/10.1007/s40626-023-00283-3}, DOI={10.1007/s40626-023-00283-3}, journal={THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY}, publisher={Springer Science and Business Media LLC}, author={Freitas, Rafael S. and Oliveira, Leonardo A. and McAdam, Scott A. M. and Lawson, Tracy and DaMatta, Fabio M. and Cardoso, Amanda A.}, year={2023}, month={Jul} }
 @article{coelho_silva_filho_oliveira_araújo_santos farnese_araújo_oliveira_2023, title={Bioaccumulation and physiological traits qualify Pistia stratiotes as a suitable species for phytoremediation and bioindication of iron-contaminated water}, url={http://dx.doi.org/10.1016/j.jhazmat.2022.130701}, DOI={10.1016/j.jhazmat.2022.130701}, abstractNote={Serious concerns have recently been raised regarding the association of Fe excess with neurodegenerative diseases in mammals and nutritional and oxidative disorders in plants. Therefore, the current study aimed to understand the physiological changes induced by Fe excess in Pistia stratiotes, a species often employed in phytoremediation studies. P. stratiotes were subjected to five concentrations of Fe: 0.038 (control), 1.0, 3.0, 5.0 and 7.0 mM. Visual symptoms of Fe-toxicity such as bronzing of leaf edges in 5.0 and 7.0 mM-grown plants were observed after 5 days. Nevertheless, no major changes were observed in photosynthesis-related parameters at this time-point. In contrast, plants growing for 10 days in high Fe concentrations showed decreased chlorophyll concentrations and lower net CO2 assimilation rate. Notwithstanding, P. stratiotes accumulated high amounts of Fe, especially in roots (maximum of 10,000 µg g-1 DW) and displayed a robust induction of the enzymatic antioxidant system. In conclusion, we demonstrated that P. stratiotes can be applied to clean up Fe-contaminated water, as the species displays high Fe bioaccumulation, mostly in root apoplasts, and can maintain physiological processes under Fe excess. Our results further revealed that by monitoring visual symptoms, P. stratiotes could be applied for bioindication purposes.}, journal={Journal of Hazardous Materials}, author={Coelho, Daniel Gomes and Silva, Vinicius Melo and Filho, Antonio Aristides Pereira Gomes and Oliveira, Leonardo Araujo and Araújo, Hugo Humberto and Santos Farnese, Fernanda and Araújo, Wagner L. and Oliveira, Juraci Alves}, year={2023}, month={Mar} }
 @article{pita-barbosa_oliveira_barros_hodecker_oliveira_araújo_martins_2023, title={Developing a Roadmap to Define a Potential Ideotype for Drought Tolerance in Eucalyptus}, url={https://doi.org/10.1093/forsci/fxac044}, DOI={10.1093/forsci/fxac044}, abstractNote={Abstract Climate change is escalating the water crisis worldwide, leading to unpredictable water availability and water scarcity, which is among the main environmental factors affecting plant growth and yield, severely affecting the productivity of planted forests, most of them composed of Eucalyptus species. The selection of genotypes capable of growing and surviving under water scarcity is required for eucalypt cultivation in areas with seasonal or continuous drought episodes. To achieve this goal, there is the current need for the identification of potential drought tolerance bioindicators, which is a challenging task since plant responses to drought involve numerous genes and strategies. We posit that eco-physiological and morphoanatomical analyses in combination with molecular approaches could help fill the current knowledge gap. In this review, we summarize recent advances in the identification of potential indicators of drought tolerance in Eucalyptus and discuss the typical responses to drought stress, as well as their importance as bioindicators for the development of a drought-tolerant eucalypt ideotype.}, journal={Forest Science}, author={Pita-Barbosa, Alice and Oliveira, Leonardo Araujo and Barros, Nairam Félix and Hodecker, Bárbara Elias Reis and Oliveira, Franciele S and Araújo, Wagner L and Martins, Samuel C V}, year={2023}, month={Feb} }
 @article{pinto_martins_fontes_oliveira_almeida_detoni_oliveira_silva_medeiros_picoli_et al._2022, title={Elevated [CO2] mitigates the impacts of heat stress in eucalyptus seedlings}, url={http://dx.doi.org/10.1007/s40626-022-00257-x}, DOI={10.1007/s40626-022-00257-x}, journal={Theoretical and Experimental Plant Physiology}, author={Pinto, Samuel S. and Martins, Auxiliadora O. and Fontes, Luiz F. P. and Oliveira, Franciele S. and Almeida, Iale C. and Detoni, Iagor B. and Oliveira, Leonardo A. and Silva, Willian B. and Medeiros, David B. and Picoli, Edgar A. T. and et al.}, year={2022}, month={Sep} }
 @article{pereira_martins_batista-silva_condori-apfata_nascimento_silva_oliveira_medeiros_martins_fernie_et al._2022, title={Elevated carbon assimilation and metabolic reprogramming in tomato high pigment mutants support the increased production of pigments}, url={http://dx.doi.org/10.1007/s00299-022-02900-y}, DOI={10.1007/s00299-022-02900-y}, journal={Plant Cell Reports}, author={Pereira, Auderlan M. and Martins, Auxiliadora O. and Batista-Silva, Willian and Condori-Apfata, Jorge A. and Nascimento, Vitor L. and Silva, Victor F. and Oliveira, Leonardo A. and Medeiros, David B. and Martins, Samuel C. V. and Fernie, Alisdair R. and et al.}, year={2022}, month={Sep} }
 @article{oliveira_cardoso_andrade_pereira_araujo_santos_damatta_martins_2022, title={Exploring leaf hydraulic traits to predict drought tolerance of Eucalyptus clones}, volume={4}, ISSN={["1758-4469"]}, url={https://doi.org/10.1093/treephys/tpac040}, DOI={10.1093/treephys/tpac040}, abstractNote={Abstract
               Ongoing changes in climate, and the consequent mortality of natural and cultivated forests across the globe, highlight the urgent need to understand the plant traits associated with greater tolerance to drought. Here, we aimed at assessing key foliar traits, with a focus on the hydraulic component, that could confer a differential ability to tolerate drought in three commercial hybrids of the most important Eucalyptus species utilized in tropical silviculture: E. urophyla, E. grandis and E. camaldulensis. All genotypes exhibited similar water potential when the 90% stomatal closure (Ψgs90) occurs with Ψgs90 always preceding the start of embolism events. The drought-tolerant hybrid showed a higher leaf resistance to embolism, but the leaf hydraulic efficiency was similar among all genotypes. Other traits presented by the drought-tolerant hybrid were a higher cell wall reinforcement, lower value of osmotic potential at full turgor and greater bulk modulus of elasticity. We also identified that the leaf capacitance after the turgor loss, the ratio between cell wall thickness (t) and lumen breadth (b) ratio (t/b)3, and the minimal conductance might be good proxies for screening drought-tolerant Eucalyptus genotypes. Our findings suggest that xylem resistance to embolism can be an important component of drought tolerance in Eucalyptus in addition to other traits aimed at delaying the development of high tensions in the xylem.
               Highlight Drought tolerance in tropical Eucalyptus hybrids encompasses a high leaf resistance to embolism and a suite of traits aimed at delaying the development of high tensions in the xylem.}, journal={TREE PHYSIOLOGY}, publisher={Oxford University Press (OUP)}, author={Oliveira, Leonardo A. and Cardoso, Amanda A. and Andrade, Moab T. and Pereira, Talitha S. and Araujo, Wagner L. and Santos, Gleison A. and Damatta, Fabio M. and Martins, Samuel C.}, editor={Pfautsch, SebastianEditor}, year={2022}, month={May} }
 @article{andrade_oliveira_pereira_cardoso_batista-silva_damatta_zsogon_martins_2022, title={Impaired auxin signaling increases vein and stomatal density but reduces hydraulic efficiency and ultimately net photosynthesis}, ISSN={["1460-2431"]}, url={https://doi.org/10.1093/jxb/erac119}, DOI={10.1093/jxb/erac119}, abstractNote={Abstract
               Auxins are known to regulate xylem development in plants, but their effects on water transport efficiency are poorly known. Here we used tomato plants with the diageotropica mutation (dgt), which has impaired function of a cyclophilin 1 cis–trans isomerase involved in auxin signaling, and the corresponding wild type (WT) to explore the mutation’s effects on plant hydraulics and leaf gas exchange. The xylem of the dgt mutant showed a reduced hydraulically weighted vessel diameter (Dh) (24–43%) and conduit number (25–58%) in petioles and stems, resulting in lower theoretical hydraulic conductivities (Kt); on the other hand, no changes in root Dh and Kt were observed. The measured stem and leaf hydraulic conductances of the dgt mutant were lower (up to 81%), in agreement with the Kt values; however, despite dgt and WT plants showing similar root Dh and Kt, the measured root hydraulic conductance of the dgt mutant was 75% lower. The dgt mutation increased the vein and stomatal density, which could potentially increase photosynthesis. Nevertheless, even though it had the same photosynthetic capacity as WT plants, the dgt mutant showed a photosynthetic rate c. 25% lower, coupled with a stomatal conductance reduction of 52%. These results clearly demonstrate that increases in minor vein and stomatal density only result in higher leaf gas exchange when accompanied by higher hydraulic efficiency.}, journal={JOURNAL OF EXPERIMENTAL BOTANY}, publisher={Oxford University Press (OUP)}, author={Andrade, Moab T. and Oliveira, Leonardo A. and Pereira, Talitha S. and Cardoso, Amanda A. and Batista-Silva, Willian and DaMatta, Fabio M. and Zsogon, Aguston and Martins, Samuel C.}, editor={Lawson, TracyEditor}, year={2022}, month={Mar} }
 @article{toledo picoli_oliveira_silva_souza_santos rufino_pereira_freitas ribeiro_malvicini_turello_d́alessandro sérgio contrim_et al._2021, title={Treasured exceptions: Association of morphoanatomical leaf traits with cup quality of Coffea arabica L. cv. “Catuaí”}, url={http://dx.doi.org/10.1016/j.foodres.2021.110118}, DOI={10.1016/j.foodres.2021.110118}, abstractNote={The morphoanatomical characteristics of leaves were associated with altitude, hillside, and the cup quality of coffee produced in the Matas de Minas region. Although the small magnitude, there are correlations between cup quality with altitude and morpho-anatomical traits. Despite facing the differences of management inherent to 363 sampling sites, Northwestern hillside had significant lower cup quality, whereas only stomata density (SD) and thickness of the leaf epidermis in the adaxial face (AdET) showed significant differences between hillsides. Altitude, leaf mass per area (LMA) and SD, and to a lesser extent the thickness of the leaf epidermis in the abaxial face (AbET), were correlated (Spearman’s correlation) with cup quality. Interestingly, AbET correlations were negative. Mantel’s test significant correlations were found between coffee cup quality vs. altitude, LMA and petiole phloem area (PPhA). The spatial autocorrelation was significant only with LMA. Also, SD, to a lesser extent, was associated with cup quality. Despite the complexity of the association among the environment, plant growth and development, this is the first report to associate morpho-anatomical features of the leaf with the coffee cup traits. Even with the expectation of genotype/species vs environment interactions, and the influence of other parameters associated with post-harvest, roasting and brewing, the evaluation of LMA, SD, AdET, AbET and the thickness of the palisade parenchyma (PPT) allow a novel approach to access coffee cup quality.}, journal={Food Research International}, author={Toledo Picoli, Edgard Augusto and Oliveira, Leonardo Araújo and Silva, Bruno Tavares and Souza, Genaína Aparecida and Santos Rufino, José Luís and Pereira, Antônio Alves and Freitas Ribeiro, Marcelo and Malvicini, Gian Luca and Turello, Luca and D́Alessandro Sérgio Contrim and et al.}, year={2021}, month={Mar} }
 @article{avila_cardoso_almeida_costa_machado_barbosa_souza_oliveira_batista_martins_et al._2020, title={Coffee plants respond to drought and elevated [CO2] through changes in stomatal function, plant hydraulic conductance, and aquaporin expression}, url={http://dx.doi.org/10.1016/j.envexpbot.2020.104148}, DOI={10.1016/j.envexpbot.2020.104148}, abstractNote={Rising air CO2 concentration ([CO2]) is believed to mitigate the negative impacts of global climate changes such as increased air temperatures and drought events on plant growth and survival. Nonetheless, how elevated [CO2] affects the way coffee (Coffea arabica L.) plants sense and respond to drought remains a critical unknown. In this study, potted coffee plants were cultivated under two air [CO2] (ca. 400 ppm or 700 ppm) in open top chambers under greenhouse conditions. After a 5-month exposure to [CO2] treatments, plants were submitted to a progressive, controlled soil water deficit down to 20 % soil field capacity. Under well-watered (100 % field capacity) conditions, 700-plants displayed lower whole-plant transpiration rates (T) than their 400-counterparts. Changes in T were unrelated to stomatal conductances at the leaf scale (as well as stomatal morphology) or foliar ABA levels, but they were rather associated with faster stomata closure rates upon rapid increases in vapor pressure deficit in the 700-plants. During drought, 700-plants were able to maintain higher water potentials and plant hydraulic conductances for longer in parallel to higher T than their 400-counterparts. Under elevated [CO2], the faster stomatal closure rates (irrigated conditions) or the maintenance of plant hydraulic conductances (drought conditions) were associated with higher (3 to 40-fold) transcript abundance of most aquaporin genes. Altogether, our results suggest that elevated [CO2] has marked implications on how coffee plants respond to soil water deficit, ultimately permitting 700-plants to have improved fitness under drought when compared to 400-plants.}, journal={Environmental and Experimental Botany}, author={Avila, Rodrigo T. and Cardoso, Amanda A. and Almeida, Wellington L. and Costa, Lucas C. and Machado, Kleiton L.G. and Barbosa, Marcela L. and Souza, Raylla P.B. and Oliveira, Leonardo A. and Batista, Diego S. and Martins, Samuel C.V. and et al.}, year={2020}, month={Sep} }
 @article{sturião_martinez_oliveira_jezler_jesus pereira_ventrella_carmo milagres_2020, title={Deficiency of calcium affects anatomical, biometry and nutritional status of cherry tomato}, url={http://dx.doi.org/10.1016/j.sajb.2020.05.003}, DOI={10.1016/j.sajb.2020.05.003}, abstractNote={Calcium (Ca) is one of the main problems in tomato management, due to its susceptibility to physiological disorders such as blosson end rot. This study aimed to evaluate the effects of Ca doses on the anatomy, biometry and nutritional status of cherry tomato hybrid BRS Iracema cultivated in a hydroponic system. The experiment consisted of an arrangement in time split-plot scheme, assigning Ca concentrations (0.5; 1.5; 3.0; 6.0 and 10.0 mmol L−1) in the plots and days after sowing (57; 84; 97 and 115) in the subplots. The design was completely randomized, with four replications. We performed a variance and regression analysis for the quantitative data and then a qualitative description of the biometric and anatomical characteristics. The best responses of biometric and production variables, as well as the better nutritional plant status occur with estimated concentrations from 6.0 to 7.0 mmol L−1 Ca in nutrient solution. Ca deficiency promotes anatomical changes such as phloem super development and leaf mesophyll thickness, lesions in the cortical region of the primary stem, with hypertrophied and collapsed cells and root apices shortening in relation to the first lateral roots. Consequently, lower growth and production, showing that tomato under Ca deficiency do not always express BER symptoms. The increase in Ca availability promotes an increase in Ca intake and reduction in Zn, Cu and N-total contents in the distal part of the fruits.}, journal={South African Journal of Botany}, author={Sturião, Walas Permanhane and Martinez, Herminia Emilia Prieto and Oliveira, Leonardo Araújo and Jezler, Caroline Nery and Jesus Pereira, Luana and Ventrella, Marília Contin and Carmo Milagres, Carla}, year={2020}, month={Aug} }
 @article{marçal_avila_quiroga-rojas_souza_junior_ponte_barbosa_oliveira_martins_ramalho_et al._2021, title={Elevated [CO2] benefits coffee growth and photosynthetic performance regardless of light availability}, url={http://dx.doi.org/10.1016/j.plaphy.2020.11.042}, DOI={10.1016/j.plaphy.2020.11.042}, abstractNote={Despite being evolved in shaded environments, most coffee (Coffea arabica L.) is cultivated worldwide under sparse shade or at full sunlight. Coffee is ranked as greatly responsive to climate change (CC), and shading has been considered an important management strategy for mitigating the harmful CC outcomes on the crop. However, there is no information on the effects of enhanced [CO2] (eCa) on coffee performance in response to light availability. Here, we examined how carbon assimilation and use are affected by eCa in combination with contrasting light levels. For that, greenhouse-grown plants were submitted to varying light levels (16 or 7.5 mol photons m−2 day−1) and [CO2] (ca. 380 or 740 μmol mol−1 air) over six months. We demonstrated that both high light and eCa improved growth and photosynthetic performance, independently. Despite marginal alterations in biomass partitioning, some allometric changes, such as higher root biomass-to-total leaf area and lower leaf area ratio under the combination of eCa and high light were found. Stimulation of photosynthetic rates by eCa occurred with no direct effect on stomatal and mesophyll conductances, and no signs of photosynthetic down-regulation were found irrespective of treatments. Particularly at high light, eCa led to decreases in both photorespiration rates and oxidative pressure. Overall, our novel findings suggest that eCa could tandemly act with shading to mitigate the harmful CC effects on coffee sustainability.}, journal={Plant Physiology and Biochemistry}, author={Marçal, Dinorah M.S. and Avila, Rodrigo and Quiroga-Rojas, Luisa F. and Souza, Raylla P.B. and Junior, Carlos C. Gomes and Ponte, Lucas Roani and Barbosa, Marcela L. and Oliveira, Leonardo A. and Martins, Samuel C.V. and Ramalho, José D.C. and et al.}, year={2021}, month={Jan} }
 @article{oliveira_souza_silva_rocha_toledo picoli_souza pereira_donzeles_freitas ribeiro_ferreira_2020, title={Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds}, volume={15}, url={https://doi.org/10.25186/.v15i.1704}, DOI={10.25186/.v15i.1704}, abstractNote={The endosperm of coffee beans is an important structure which is composed of different reserve compounds. In the present work, we followed anatomical features during the reserve mobilization of Coffea arabica L. ‘Catuaí’ seed samples, harvested at 20 different sites, after 0, 3, 6, 12, 24, 33 and 45 days of imbibition. Seed samples were properly fixed and stored, respectively, for histochemical and enzyme activity approach. Fixed samples were cross-sectioned for detection of starch, lipids, essential oils, oleoresins, proteins, phenolic compounds, neutral polysaccharides, alkaloids, β-1,3 and β-1,4 glucans, and lignin. Overall, based on histochemical tests and enzyme activity, seed reserve mobilization was similar among the evaluated altitudes and mountainsides. During soaking, there is an intense consumption of reserve compounds, starting at the region close to the embryo. Reserve mobilization intensifies after root protrusion, from 12 days of soaking. The intensification of the reserve consumption is concomitant with an increased activity of MDH is observed at the first 12 days, whereas SOD showed higher expression after 33 days of soaking. At the 33rd day, little reserve is observed in the endosperm. At 45th day, cotyledon leaves are expanded, and the root system, constituted by the taproot and lateral roots, is well established. Key words: Coffea arabica; germination; reserve breakdown; histochemistry; seed anatomy.}, journal={Coffee Science}, publisher={Coffee Science}, author={Oliveira, Leonardo Araujo and Souza, Genaina Aparecida and Silva, Bruno Tavares and Rocha, Antônio Augusto Gomes and Toledo Picoli, Edgard Augusto and Souza Pereira, Diego and Donzeles, Sérgio Maurício Lopes and Freitas Ribeiro, Marcelo and Ferreira, Williams Pinto Marques}, year={2020}, pages={1–14} }
 @article{mauri_cardoso_silva_oliveira_avila_martins_damatta_2020, title={Leaf hydraulic properties are decoupled from leaf area across coffee species}, url={http://dx.doi.org/10.1007/s00468-020-01983-y}, DOI={10.1007/s00468-020-01983-y}, journal={Trees}, author={Mauri, Rafael and Cardoso, Amanda A. and Silva, Mariela M. and Oliveira, Leonardo A. and Avila, Rodrigo T. and Martins, Samuel C. V. and DaMatta, Fábio M.}, year={2020}, month={Dec} }
 @article{oliveira pires_souza_santos dias_oliveira_borges_2016, title={Protective action of nitric oxide in sesame seeds submitted to water stress}, url={http://dx.doi.org/10.1590/2317-1545v38n4166972}, DOI={10.1590/2317-1545v38n4166972}, abstractNote={Abstract: The objective in this work was to investigate the effect of nitric oxide (NO) like protective agent in sesame seeds submitted to different osmotic potentials. The treatments, in total of eight, were water (control), water plus sodium nitroprusside (SNP) and the other treatments with PEG 6000 and PEG 6000 plus SNP: - 0.1 MPa, -0.1MPa +200 µM of SNP, 0.2 MPa, -0.2 MPa +200 µM of SNP, -0.3 MPa and -0.3 MPa, +200 µM of SNP. Were done the following determinations: germination, first count of germination, speed germination index, hypocotyl length, radicle length, dry mass of hypocotyl and radicle. It was quantified the activity of the antioxidative enzymes, superoxide dismutase, catalase, ascorbate peroxidase and total peroxidase. The experimental design was completely randomized with five replications. The water restriction reduced the germination of sesame seeds, however, the presence of nitric oxide (NO) due to the application of SNP, was beneficial, promoting increase in germination, vigor and seedlings. There was an increase of antioxidative enzymes activity in the period of 0 to 24 hours, demonstrating organization of antioxidative system in all long the time. The association of PEG 6000 to SNP, increased the activity of antioxidative enzymes, evidencing an efficient system of elimination of ROS formed during the exposition to water deficit.}, journal={Journal of Seed Science}, author={Oliveira Pires, Raquel Maria and Souza, Genaina Aparecida and Santos Dias, Denise Cunha Fernandes and Oliveira, Leonardo Araujo and Borges, Eduardo Euclydes}, year={2016}, month={Dec} }