Avery Roberts

College of Agriculture and Life Sciences

Works (4)

Updated: July 31st, 2024 05:04

2024 article

Characterization of <i>Ligilactobacillus salivarius</i> CRISPR-Cas systems

Roberts, A., Spang, D., Sanozky-Dawes, R., Nethery, M. A., & Barrangou, R. (2024, July 11). (C. D. Ellermeier, Ed.). MSPHERE, Vol. 7.

By: A. Roberts n, D. Spang n, R. Sanozky-Dawes n, M. Nethery n & R. Barrangou n 

Ed(s): C. Ellermeier

author keywords: Ligilactobacillus; salivarius; CRISPR; Cas
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: July 15, 2024

2022 journal article

CRISPR-based engineering of phages for in situ bacterial base editing

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(46).

By: M. Nethery n, C. Hidalgo-Cantabrana  n, A. Roberts n & R. Barrangou n 

author keywords: phage engineering; CRISPR; base editing; soil microbiome
MeSH headings : Gene Editing; CRISPR-Cas Systems / genetics; Bacteriophages / genetics; Ecosystem; Soil
TL;DR: Phage-assisted delivery, together with base editing, offers an important in situ editing approach for the genetic interrogation of microbial community members. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 8, 2022

2022 article

Functional characterization of diverse type I-F CRISPR-associated transposons

Roberts, A., Nethery, M. A., & Barrangou, R. (2022, November 17). NUCLEIC ACIDS RESEARCH, Vol. 11.

By: A. Roberts n, M. Nethery n & R. Barrangou n 

MeSH headings : CRISPR-Cas Systems; Plasmids; Endonucleases / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; DNA; RNA; CRISPR-Associated Proteins / genetics; CRISPR-Associated Proteins / metabolism
TL;DR: This work selected a diverse set of type I-F3 CRISPR-associated transposon systems derived from Gammaproteobacteria, predicted all components essential for transposition activity, and deployed them for functionality testing within Escherichia coli, demonstrating that these systems possess a significant range of integration efficiencies with regards to temperature, transposition size, and flexible PAM requirements. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 19, 2022

2022 journal article

Genomic and epigenetic landscapes drive CRISPR-based genome editing in Bifidobacterium

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(30).

By: M. Pan  n, W. Morovic *, C. Hidalgo-Cantabrana  n, A. Roberts n, K. Walden *, Y. Goh n, R. Barrangou n 

author keywords: CRISPR-Cas; bifidobacterium; genomics; epigenomics; probiotics
MeSH headings : Bifidobacterium / genetics; CRISPR-Cas Systems; Gene Editing / methods; Genome, Bacterial / genetics; Genomics; Humans; Probiotics
TL;DR: This study adapted existing CRISPR effectors and editing strategies to generate a variety of editing outcomes in B. animalis subsp. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: July 21, 2022

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