Avery Roberts

College of Agriculture and Life Sciences

Works (4)

Updated: July 31st, 2024 05:04

2024 article

Characterization of <i>Ligilactobacillus salivarius</i> CRISPR-Cas systems

Roberts, A., Spang, D., Sanozky-Dawes, R., Nethery, M. A., & Barrangou, R. (2024, July 11). (C. D. Ellermeier, Ed.). MSPHERE, Vol. 7.

By: A. Roberts n, D. Spang n, R. Sanozky-Dawes n, M. Nethery n & R. Barrangou n 

Ed(s): C. Ellermeier

author keywords: Ligilactobacillus; salivarius; CRISPR; Cas
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: July 15, 2024

2022 journal article

CRISPR-based engineering of phages for in situ bacterial base editing

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(46).

By: M. Nethery n, C. Hidalgo-Cantabrana  n, A. Roberts n & R. Barrangou n 

author keywords: phage engineering; CRISPR; base editing; soil microbiome
MeSH headings : Gene Editing; CRISPR-Cas Systems / genetics; Bacteriophages / genetics; Ecosystem; Soil
TL;DR: Phage-assisted delivery, together with base editing, offers an important in situ editing approach for the genetic interrogation of microbial community members. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 8, 2022

2022 article

Functional characterization of diverse type I-F CRISPR-associated transposons

Roberts, A., Nethery, M. A., & Barrangou, R. (2022, November 17). NUCLEIC ACIDS RESEARCH, Vol. 11.

By: A. Roberts n, M. Nethery n & R. Barrangou n 

MeSH headings : CRISPR-Cas Systems; Plasmids; Endonucleases / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; DNA; RNA; CRISPR-Associated Proteins / genetics; CRISPR-Associated Proteins / metabolism
TL;DR: This work selected a diverse set of type I-F3 CRISPR-associated transposon systems derived from Gammaproteobacteria, predicted all components essential for transposition activity, and deployed them for functionality testing within Escherichia coli, demonstrating that these systems possess a significant range of integration efficiencies with regards to temperature, transposition size, and flexible PAM requirements. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 19, 2022

2022 journal article

Genomic and epigenetic landscapes drive CRISPR-based genome editing in Bifidobacterium

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(30).

By: M. Pan  n, W. Morovic *, C. Hidalgo-Cantabrana  n, A. Roberts n, K. Walden *, Y. Goh n, R. Barrangou n 

author keywords: CRISPR-Cas; bifidobacterium; genomics; epigenomics; probiotics
MeSH headings : Bifidobacterium / genetics; CRISPR-Cas Systems; Gene Editing / methods; Genome, Bacterial / genetics; Genomics; Humans; Probiotics
TL;DR: This study adapted existing CRISPR effectors and editing strategies to generate a variety of editing outcomes in B. animalis subsp. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: July 21, 2022

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