A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses
Marks, M. D., Betancur, L., Gilding, E., Chen, F., Bauer, S., Wenger, J. P., … Haigler, C. H. (2008, July 5). The Plant Journal, Vol. 56, pp. 483–492.
author keywords: trichome isolation; trichome cell wall; trichome gene expression; lignin; pectin
MeSH headings : Arabidopsis / chemistry; Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis Proteins / isolation & purification; Cell Separation / methods; Cell Wall / chemistry; DNA, Plant / genetics; DNA, Plant / isolation & purification; Gene Expression Profiling; Genes, Plant; Genome, Plant; Lignin / chemistry; Microscopy, Electron, Transmission; Monosaccharides / chemistry; Plant Epidermis / cytology; Plant Epidermis / genetics; Plant Leaves / cytology; Plant Leaves / genetics; Polymerase Chain Reaction; RNA, Plant / genetics; RNA, Plant / isolation & purification
topics (OpenAlex): Polysaccharides and Plant Cell Walls; Plant Molecular Biology Research; Plant nutrient uptake and metabolism
TL;DR:
The monosaccharide analysis and positive staining with ruthenium red indicates that the walls also contain a large portion of pectin, which suggests that the polysaccharides of the trichome cell walls are more like those of typical primary walls even though the wall becomes quite thick.
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