@article{betancur_singh_rapp_wendel_marks_roberts_haigler_2010, title={Phylogenetically Distinct Cellulose Synthase Genes Support Secondary Wall Thickening in Arabidopsis Shoot Trichomes and Cotton Fiber}, volume={52}, ISSN={1672-9072 1744-7909}, url={http://dx.doi.org/10.1111/j.1744-7909.2010.00934.x}, DOI={10.1111/j.1744-7909.2010.00934.x}, abstractNote={Abstract Through exploring potential analogies between cotton seed trichomes (or cotton fiber) and arabidopsis shoot trichomes we discovered that CesAs from either the primary or secondary wall phylogenetic clades can support secondary wall thickening. CesA genes that typically support primary wall synthesis, AtCesA1,2,3,5, and 6, underpin expansion and secondary wall thickening of arabidopsis shoot trichomes. In contrast, apparent orthologs of CesA genes that support secondary wall synthesis in arabidopsis xylem, AtCesA4,7, and 8, are up‐regulated for cotton fiber secondary wall deposition. These conclusions arose from: (a) analyzing the expression of CesA genes in arabidopsis shoot trichomes; (b) observing birefringent secondary walls in arabidopsis shoot trichomes with mutations in AtCesA4, 7, or 8; (c) assaying up‐regulated genes during different stages of cotton fiber development; and (d) comparing genes that were co‐expressed with primary or secondary wall CesAs in arabidopsis with genes up‐regulated in arabidopsis trichomes, arabidopsis secondary xylem, or cotton fiber during primary or secondary wall deposition. Cumulatively, the data show that: (a) the xylem of arabidopsis provides the best model for secondary wall cellulose synthesis in cotton fiber; and (b) CesA genes within a “cell wall toolbox” are used in diverse ways for the construction of particular specialized cell walls. }, number={2}, journal={Journal of Integrative Plant Biology}, publisher={Wiley}, author={Betancur, Lissete and Singh, Bir and Rapp, Ryan A. and Wendel, Jonathan F. and Marks, M. David and Roberts, Alison W. and Haigler, Candace H.}, year={2010}, month={Feb}, pages={205–220} }
 @article{marks_betancur_gilding_chen_bauer_wenger_dixon_haigler_2008, title={A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses}, volume={56}, ISSN={["0960-7412"]}, DOI={10.1111/j.1365-313X.2008.03611.x}, abstractNote={SummaryA new procedure has been developed for the isolation of wild‐type and mutant Arabidopsis trichomes. The isolated trichomes maintained enzymatic activity and were used for DNA, protein, and RNA isolation. The RNA was used to generate probes suitable for Affymetrix analysis. The validity of the Affymetrix results was confirmed by quantitative PCR analysis on a subset of genes that are preferentially expressed in trichomes or leaves. Sufficient quantities of trichomes were isolated to probe the biochemical nature of trichome cell walls. These analyses provide evidence for the presence of lignin in Arabidopsis trichome cell walls. The monosaccharide analysis and positive staining with ruthenium red indicates that the walls also contain a large portion of pectin. The 2.23‐fold ratio of pectin‐related sugars compared with potential cellulosic glucose suggests that the polysaccharides of the trichome cell walls are more like those of typical primary walls even though the wall becomes quite thick. Overall, these analyses open the door to using the Arabidopsis trichome cell wall as an excellent model to probe various questions concerning plant cell wall biosynthesis.}, number={3}, journal={PLANT JOURNAL}, author={Marks, M. David and Betancur, Lissete and Gilding, Edward and Chen, Fang and Bauer, Stefan and Wenger, Jonathan P. and Dixon, Richard A. and Haigler, Candace H.}, year={2008}, month={Nov}, pages={483–492} }