Ling Chuang

Works (3)

Updated: August 8th, 2023 21:20

2015 journal article

Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis

Proceedings of the National Academy of Sciences, 112(27), 8481–8486.

By: J. Wang n , L. Chuang n, P. Loziuk n, H. Chen n, Y. Lin n, R. Shi n, G. Qu *, D. Muddiman n , R. Sederoff n, V. Chiang n

Contributors: J. Wang n , L. Chuang n, P. Loziuk n, H. Chen n, Y. Lin n, R. Shi n, G. Qu *, D. Muddiman n , R. Sederoff n, V. Chiang n

author keywords: AldOMT; COMT; lignin; phosphoproteomics; phosphoregulation
MeSH headings : Acrolein / analogs & derivatives; Acrolein / metabolism; Amino Acid Sequence; Binding Sites / genetics; Biocatalysis; Catechols / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Isoenzymes / genetics; Isoenzymes / metabolism; Lignin / biosynthesis; Methyltransferases / genetics; Methyltransferases / metabolism; Microscopy, Confocal; Molecular Sequence Data; Mutation; Phosphoproteins / metabolism; Phosphorylation; Plant Proteins / genetics; Plant Proteins / metabolism; Populus / enzymology; Populus / genetics; Populus / metabolism; Proteomics / methods; Protoplasts / enzymology; Protoplasts / metabolism; Sequence Homology, Amino Acid; Tandem Mass Spectrometry
topics (OpenAlex): Plant Gene Expression Analysis; Biofuel production and bioconversion; Horticultural and Viticultural Research
TL;DR: It is shown that reversible monophosphorylation at Ser123 or Ser125 acts as an on/off switch for the activity of 5-hydroxyconiferaldehyde O-methyltransferase 2 (PtrAldOMT2), an enzyme central to monolignol biosynthesis for lignification in stem-differentiating xylem of Populus trichocarpa. (via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2014 article

A robust chromatin immunoprecipitation protocol for studying transcription factor–DNA interactions and histone modifications in wood-forming tissue

Li, W., Lin, Y.-C., Li, Q., Shi, R., Lin, C.-Y., Chen, H., … Chiang, V. L. (2014, August 21). Nature Protocols.

By: W. Li n, Y. Lin n, Q. Li n, R. Shi n, C. Lin  n, H. Chen n, L. Chuang n, G. Qu *, R. Sederoff n, V. Chiang n

MeSH headings : Base Sequence; Chromatin Immunoprecipitation / methods; DNA / metabolism; DNA Fragmentation; Histones / metabolism; Immunoprecipitation; Molecular Sequence Data; Populus / cytology; Populus / metabolism; Sequence Analysis, DNA; Transcription Factors / metabolism; Wood / cytology; Xylem / genetics; Xylem / metabolism
topics (OpenAlex): Plant Molecular Biology Research; Genomics and Chromatin Dynamics; Plant Gene Expression Analysis
TL;DR: This protocol is the only working ChIP system for wood-forming tissue and uses stem-differentiating xylem to identify genome-wide specific TF-DNA interactions during wood formation and histone modifications associated with regulation of wood formation. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2014 article

A simple improved-throughput xylem protoplast system for studying wood formation

Lin, Y.-C., Li, W., Chen, H., Li, Q., Sun, Y.-H., Shi, R., … Chiang, V. L. (2014, August 21). Nature Protocols, Vol. 9, pp. 2194–2205.

By: Y. Lin n, W. Li n, H. Chen n, Q. Li n, Y. Sun *, R. Shi n, C. Lin n, J. Wang n  ...

Contributors: Y. Lin n, W. Li n, H. Chen n, Q. Li n, Y. Sun *, R. Shi n, C. Lin n, J. Wang n  ...

MeSH headings : Cell Wall / metabolism; Gene Expression Regulation, Plant / genetics; Gene Regulatory Networks / genetics; Green Fluorescent Proteins; Populus / cytology; Populus / growth & development; Protoplasts / metabolism; Transfection / methods; Wood / cytology; Wood / growth & development; Xylem / chemistry
topics (OpenAlex): Plant Molecular Biology Research; Plant Reproductive Biology; Plant Gene Expression Analysis
TL;DR: A protocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-differentiating xylem (SDX), in the model woody plant Populus trichocarpa is presented. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

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