Development of a tandem affinity phosphoproteomic method with motif selectivity and its application in analysis of signal transduction networks
Herring, L. E., Grant, K. G., Blackburn, K., Haugh, J. M., & Goshe, M. B. (2015, February 19). Journal of Chromatography B, Vol. 988, pp. 166–174.
author keywords: Phosphopeptide enrichment; Liquid chromatography; Mass spectrometry; Motif analysis; Pathway analysis
MeSH headings : Amino Acid Sequence; Chromatography, Liquid / methods; Molecular Sequence Data; Phosphopeptides / analysis; Phosphopeptides / chemistry; Proteomics / methods; Signal Transduction / physiology; Tandem Mass Spectrometry / methods
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Protein Kinase Regulation and GTPase Signaling; Enzyme Structure and Function
TL;DR:
A multi-dimensional method incorporating electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) with tandem IMAC/TiO2 enrichment for subsequent phosphopeptide identification by LC/MS/MS and biological functions that were over-represented at each PDGF stimulation time point illustrate the feasibility of this approach in quantitative phosphoproteomic studies.
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