Laura Kimberly Gardner

author keywords: chemical biology; photocontrol; riboswitches; RNA; synthetic biology

MeSH headings : Cell Membrane Permeability; Gene Expression Regulation / drug effects; Gene Expression Regulation / radiation effects; Intracellular Space / drug effects; Intracellular Space / genetics; Intracellular Space / radiation effects; Ligands; Molecular Structure; Riboswitch / drug effects; Riboswitch / genetics; Riboswitch / radiation effects; Structure-Activity Relationship; Theophylline / chemistry; Theophylline / pharmacology; Ultraviolet Rays

TL;DR: By combining a riboswitch with a cell‐permeable photocaged small‐molecule ligand, an optochemical gene control element was constructed that enabled spatial and temporal control of gene expression in bacterial cells. (via Semantic Scholar)

MeSH headings : Animals; Cell Line; Gene Regulatory Networks / drug effects; Gene Regulatory Networks / radiation effects; Genetic Engineering / methods; Humans; Light; Photochemical Processes

TL;DR: Recent advancements in the area of light-controlled synthetic networks are described, which allows for the regulation of intermediate steps of complex dynamic pathways in mammalian cells such as those involved in kinase networks. (via Semantic Scholar)

MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Drug Resistance, Bacterial / drug effects; Drug Resistance, Bacterial / genetics; Erythromycin / pharmacology; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli / radiation effects; Exoribonucleases / genetics; Exoribonucleases / metabolism; Light; Photochemistry / methods

TL;DR: This work engineered the MphR(A)/promoter system to enable photochemical activation of gene expression in E. coli, and applied this photochemical gene switch to the construction of a light-triggered logic gate, aLight-controlled band-pass filter, as well as spatial and temporal control of geneexpression. (via Semantic Scholar)