@article{kraft_sit_diepenbrock_ashrafi_aryal_fernandez_burrack_2021, title={Detection of Fruit Meals Within Laboratory-Raised and Field-Trapped Adult Drosophila suzukii (Diptera: Drosophilidae) Guts}, volume={9}, ISSN={2296-701X}, url={http://dx.doi.org/10.3389/fevo.2021.719645}, DOI={10.3389/fevo.2021.719645}, abstractNote={The feeding habits of adult Brachycera are understudied and may provide important context for understanding invasive pest biology, as with the polyphagous small fruit pest Drosophila suzukii. We developed molecular methods to study adult D. suzukii gut content in order to understand its feeding habits. We designed and verified two primer pairs specific for either blueberries or blackberries and used a qPCR melt curve analysis to determine whether we can detect the presence or absence of berry feeding by adult flies. In a laboratory assay, the blueberry fly meal DNA can be detected for longer periods than the blackberry meal DNA. Generally, female gut contents are less variable than male gut contents. We also tested recently emerged flies that were not fed as adults but developed as larvae in either blueberries or blackberries. Some adult flies from each fruit had detectable fruit DNA in their gut, which could be due to pupal meconium feeding after emergence. Next, we aimed to test the primers in the field to develop techniques to track fruit feeding by D. suzukii in its natural field environment. First, to identify the most appropriate collection method, we determined how long we could detect fruit DNA, using previously developed primers within D. suzukii gut preserved in four types of trap fluid in the laboratory. The likelihood of detecting blackberry DNA differed by day, trap fluid, and between sexes. For the blueberry primer, the possibility of detecting blueberry DNA differed by trap fluid only. Based on those results, we used RV antifreeze with a Scentry SWD lure in field trials at two research station locations, one containing blackberries and one with blueberries. We established transects away from each fruit planting and collected up to 120 total flies at each point along transects. There were no significant differences in the number of flies containing berry DNA among collection points along the transect in both locations. These results suggest that adult flies move between crop and non-crop habitats and may not be highly dependent on fruit food resources.}, journal={Frontiers in Ecology and Evolution}, publisher={Frontiers Media SA}, author={Kraft, Laura J. and Sit, Tim L. and Diepenbrock, Lauren M. and Ashrafi, Hamid and Aryal, Rishi and Fernandez, Gina E. and Burrack, Hannah J.}, year={2021}, month={Aug} }
 @article{olazcuaga_loiseau_parrinello_paris_fraimout_guedot_diepenbrock_kenis_zhang_chen_et al._2020, title={A Whole-Genome Scan for Association with Invasion Success in the Fruit Fly Drosophila suzukii Using Contrasts of Allele Frequencies Corrected for Population Structure}, volume={37}, ISSN={["1537-1719"]}, DOI={10.1093/molbev/msaa098}, abstractNote={Abstract}, number={8}, journal={MOLECULAR BIOLOGY AND EVOLUTION}, author={Olazcuaga, Laure and Loiseau, Anne and Parrinello, Hugues and Paris, Mathilde and Fraimout, Antoine and Guedot, Christelle and Diepenbrock, Lauren M. and Kenis, Marc and Zhang, Jinping and Chen, Xiao and et al.}, year={2020}, month={Aug}, pages={2369–2385} }
 @article{diepenbrock_mcphie_2018, title={Use of a novel host plant family, Basallaceae, by Drosophila suzukii (Diptera: Drosophilidae)}, volume={120}, number={2}, journal={Proceedings of the Entomological Society of Washington}, author={Diepenbrock, L. M. and McPhie, D. R.}, year={2018}, pages={255–259} }
 @article{van timmeren_diepenbrock_bertone_burrack_isaacs_2017, title={A Filter Method for Improved Monitoring of Drosophila suzukii (Diptera: Drosophilidae) Larvae in Fruit}, volume={8}, ISSN={["2155-7470"]}, DOI={10.1093/jipm/pmx019}, abstractNote={Drosophila suzukii Matsumura (Diptera: Drosophilidae) has become a major economic pest of soft-skinned fruits since it was detected in North America in 2008. Control of this fly is achieved through insecticide sprays applied when ripening or ripe fruit are present. Monitoring to aid informed management decisions is challenging since trapping for adults is not a reliable indicator of potential or existing infestation in the fruit. Moreover, current larval monitoring techniques using brown sugar or salt solutions allow for visual detection of late-instar larvae, but they are time consuming and tend to miss smaller larvae. Here, we describe a method combining a salt solution, coffee filter, and microscope that can reliably and efficiently detect small and large larvae of D. suzukii in fruit samples. By sifting the sample liquid through an inexpensive coffee filter, larvae of all instars can be counted quickly and accurately. This method is 1.7 times faster than using a visual traybased method and can detect more larvae because first instar larvae can be detected. Growers can use this information to target insecticide sprays for curative control of small larvae, identify fields where the presence of larger larvae may indicate an unmarketable crop, or verify that no infestation exists and control programs are working. We provide images to support larval identification of this pest, and we expect this method will become an important component of rebuilding IPM programs in fruit crops affected by D. suzukii.}, number={1}, journal={JOURNAL OF INTEGRATED PEST MANAGEMENT}, author={Van Timmeren, Steven and Diepenbrock, Lauren M. and Bertone, Matthew A. and Burrack, Hannah J. and Isaacs, Rufus}, year={2017}, month={Jan} }
 @article{diepenbrock_hardin_burrack_2017, title={Season-long programs for control of Drosophila suzukii in southeastern US blackberries}, volume={98}, ISSN={["1873-6904"]}, DOI={10.1016/j.cropro.2017.03.022}, abstractNote={Drosophila suzukii is an invasive insect pest which impacts small fruit production throughout much of the world. Current management programs use regular applications of broad-spectrum insecticides which must be rotated for resistance management. This study examined the efficacy of rotational treatment programs designed to meet the needs of commercial growers in the southeastern United States, a region which experiences frequent rainfall during the growing season. In bioassays, all insecticides in our programs killed at least 50% of all female flies. Despite this good efficacy and weekly applications, infestation still occurred within fields. Our findings demonstrate the necessity of a comprehensive management strategy for blackberries, requiring additional efforts to current chemical-intensive management regimes, including cultural management practices such as pruning, harvest frequency, and post-harvest cooling.}, journal={CROP PROTECTION}, author={Diepenbrock, Lauren M. and Hardin, Jesse A. and Burrack, Hannah J.}, year={2017}, month={Aug}, pages={149–156} }
 @article{diepenbrock_burrack_2017, title={Variation of within-crop microhabitat use by Drosophila suzukii (Diptera: Drosophilidae) in blackberry}, volume={141}, ISSN={["1439-0418"]}, DOI={10.1111/jen.12335}, abstractNote={Abstract}, number={1-2}, journal={JOURNAL OF APPLIED ENTOMOLOGY}, author={Diepenbrock, L. M. and Burrack, H. J.}, year={2017}, month={Feb}, pages={1–7} }
 @article{diepenbrock_rosensteel_hardin_sial_burrack_2016, title={Season-long programs for control of Drosophila suzukii in southeastern US blueberries}, volume={81}, ISSN={["1873-6904"]}, DOI={10.1016/j.cropro.2015.12.012}, abstractNote={Drosophila suzukii is an invasive insect pest which has major impacts on small fruit production throughout North America. Current control programs use frequent applications of broad-spectrum insecticides. To prevent resistance, modes of action must be rotated. This study examines the acute and residual efficacy of rotational treatment programs designed to meet the needs of commercial growers in the southeastern United States, a region which experiences significant precipitation throughout the growing season. All insecticide applications had greater adult acute mortality than controls, and some had residual efficacy for three or more days after treatment. Programs had no residual effects at seven days after treatment. Within programs, organophosphates (phosmet and malathion) and pyrethroids (zeta-cypermethrin and fenpropathrin) were the most effective. Some programs also resulted in less offspring production by D. suzukii in bioassays and reduced field infestation compared to controls. Results show that effective, season-long rotational chemical treatment programs can be designed to minimize crop damage, meet exportation requirements and manage for resistance. Understanding the effect of regional climate on the performance of individual insecticide treatments and their efficacy will be critical for widespread implementation of effective control programs for D. suzukii.}, journal={CROP PROTECTION}, author={Diepenbrock, Lauren M. and Rosensteel, Danielle Olivieri and Hardin, Jesse A. and Sial, Ashfaq A. and Burrack, Hannah J.}, year={2016}, month={Mar}, pages={76–84} }
 @article{diepenbrock_rosensteel_hardin_sial_burrack_2016, title={Season-long programs for control of Drosophila suzukii in southeastern blueberries (vol 81, pg 76, 2016)}, volume={84}, ISSN={["1873-6904"]}, DOI={10.1016/j.cropro.2016.02.010}, journal={CROP PROTECTION}, author={Diepenbrock, Lauren M. and Rosensteel, Danielle Olivieri and Hardin, Jesse A. and Sial, Ashfaq A. and Burrack, Hannah J.}, year={2016}, month={Jun}, pages={171–171} }