@article{singh_hung_shanahan_kanke_bonfini_dame_biraud_peck_oyesola_freund_et al._2020, title={Enteroendocrine Progenitor Cell-Enriched mir-7 Regulates Intestinal Epithelial Proliferation in an Xiap-Dependent Manner}, volume={9}, ISBN={2352-345X}, DOI={10.1016/j.jcmgh.2019.11.001}, number={3}, journal={CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY}, author={Singh, Ajeet P. and Hung, Yu-Han and Shanahan, Michael T. and Kanke, Matt and Bonfini, Alessandro and Dame, Michael K. and Biraud, Mandy and Peck, Bailey C. E. and Oyesola, Oyebola O. and Freund, John M. and et al.}, year={2020}, pages={447–464} }
 @article{orr_baker_lynch_hughes_clark_slone_fogle_gonzalez_2020, title={Prognostic value of colonic and peripheral venous lactate measurements in horses with large colon volvulus}, volume={1}, DOI={10.1111/vsu.13361}, journal={Veterinary Surgery}, publisher={Wiley}, author={Orr, Kindra. E. and Baker, W. True and Lynch, Tim M. and Hughes, Faith E. and Clark, Carol K. and Slone, Donnie E., Jr and Fogle, Callie A. and Gonzalez, Liara M.}, year={2020}, month={Apr} }
 @article{gonzalez_stewart_freund_kucera_dekaney_magness_blikslager_2019, title={Preservation of reserve intestinal epithelial stem cells following severe ischemic injury}, volume={316}, DOI={10.1152/ajpgi.00262.2018}, number={4}, journal={American Journal of Physiology-Gastrointestinal and Liver Physiology}, publisher={American Physiological Society}, author={Gonzalez, Liara M. and Stewart, Amy Stieler and Freund, John and Kucera, Cecilia Renee and Dekaney, Christopher M. and Magness, Scott T. and Blikslager, Anthony T.}, year={2019}, month={Apr}, pages={G482–G494} }
 @article{ziegler_pridgen_mills_gonzalez_landeghem_odle_blikslager_2018, title={Epithelial restitution defect in neonatal jejunum is rescued by juvenile mucosal homogenate in a pig model of intestinal ischemic injury and repair}, volume={13}, DOI={10.1371/journal.pone.0200674}, number={8}, journal={PLOS ONE}, author={Ziegler, Amanda L. and Pridgen, Tiffany A. and Mills, Juliana K. and Gonzalez, Liara M. and Landeghem, Laurianne Van and Odle, Jack and Blikslager, Anthony T.}, editor={Karhausen, JörnEditor}, year={2018}, month={Aug} }
 @article{blikslager_gonzalez_2018, title={Equine intestinal mucosal pathobiology}, volume={6}, journal={Annual review of animal biosciences, vol 6}, author={Blikslager, A. and Gonzalez, L.}, year={2018}, pages={157–175} }
 @article{fletcher_mansell_martin_borst_barnes_gonzalez_2018, title={Gross Morphometry, Histomorphometry, and Immunohistochemistry Confirm Early and Persistent Jejunal Crypt Hyperplasia in Poults with Enteritis and Depressed Growth}, volume={62}, ISSN={0005-2086 1938-4351}, url={http://dx.doi.org/10.1637/11759-101717-reg.1}, DOI={10.1637/11759-101717-reg.1}, number={2}, journal={Avian Diseases}, publisher={American Association of Avian Pathologists (AAAP)}, author={Fletcher, O. J. and Mansell, R. and Martin, M. P. and Borst, L. B. and Barnes, H. John and Gonzalez, L. M.}, year={2018}, month={Jun}, pages={163–170} }
 @article{stewart_freund_blikslager_gonzalez_2018, title={Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia}, DOI={10.3791/57647}, number={135}, journal={JOVE-JOURNAL OF VISUALIZED EXPERIMENTS}, author={Stewart, Amy Stieler and Freund, John M. and Blikslager, Anthony and Gonzalez, Liara}, year={2018} }
 @article{gonzalez_2018, title={Large colon volvulus: The continued conundrum}, volume={3}, DOI={10.1111/eve.12748}, number={6}, journal={Equine Veterinary Education}, publisher={Wiley}, author={Gonzalez, L. M.}, year={2018}, month={Jun}, pages={312–314} }
 @article{kucera_stranahan_hughes_blikslager_gonzalez_2018, title={Protein biomarker of cell proliferation determines survival to discharge in cases of equine large colon volvulus}, volume={50}, DOI={10.1111/evj.12767}, number={4}, journal={Equine Veterinary Journal}, publisher={Wiley}, author={Kucera, C. R. and Stranahan, L. W. and Hughes, F. and Blikslager, A. T. and Gonzalez, L. M.}, year={2018}, month={Jul}, pages={452–456} }
 @article{lima_lin_jacobi_man_sommer_flowers_blikslager_gonzalez_odle_2018, title={Supplementation of maternal diets with docosahexaenoic acid and methylating vitamins impacts growth and development of fetuses from malnourished gilts}, volume={2}, DOI={10.3945/cdn.117.001958}, number={3}, journal={Current Developments in Nutrition}, author={Lima, H. K. and Lin, X. and Jacobi, S. K. and Man, C. L. and Sommer, J. and Flowers, W. and Blikslager, Anthony and Gonzalez, Liara and Odle, J.}, year={2018} }
 @article{royal_hunt_gonzalez_lewbart_bailey_2018, title={Veterinary Medical Students' Motivations for Exercise}, volume={45}, ISSN={0748-321X 1943-7218}, url={http://dx.doi.org/10.3138/jvme.0117-004r}, DOI={10.3138/jvme.0117-004r}, number={3}, journal={Journal of Veterinary Medical Education}, publisher={University of Toronto Press Inc. (UTPress)}, author={Royal, Kenneth D. and Hunt, Suzanne A. and Gonzalez, Liara M. and Lewbart, Gregory A. and Bailey, Kate M.}, year={2018}, month={Aug}, pages={367–373} }
 @article{royal_hunt_gonzalez_lewbart_bailey_2018, title={Veterinary Medical Students' Motivations for Exercise}, volume={1}, ISSN={0748-321X 1943-7218}, url={http://dx.doi.org/10.3138/jvme.0117-004}, DOI={10.3138/jvme.0117-004}, journal={Journal of Veterinary Medical Education}, publisher={University of Toronto Press Inc. (UTPress)}, author={Royal, Kenneth D. and Hunt, Suzanne A. and Gonzalez, Liara M. and Lewbart, Gregory A. and Bailey, Kate M.}, year={2018}, month={Jan}, pages={1–7} }
 @article{dellon_joshi_blikslager_gonzalez_pridgen_whitlow_ivanovic_slaughter_garman_karp_et al._2017, title={A Novel Inflammation-Activated Drug Delivery System Using Self-Assembling Hydrogel Doubles Esophageal Dwell Time in an Esophageal Injury Porcine Model}, volume={152}, ISSN={0016-5085}, url={http://dx.doi.org/10.1016/S0016-5085(17)32956-6}, DOI={10.1016/S0016-5085(17)32956-6}, number={5}, journal={Gastroenterology}, publisher={Elsevier BV}, author={Dellon, Evan S. and Joshi, Nitin and Blikslager, Anthony and Gonzalez, Liara M. and Pridgen, Tiffany and Whitlow, Ann and Ivanovic, Marija and Slaughter, Kai and Garman, Katherine S. and Karp, Jeffrey M. and et al.}, year={2017}, month={Apr}, pages={S859–S860} }
 @article{stewart_freund_gonzalez_2017, title={Advanced three-dimensional culture of equine intestinal epithelial stem cells}, volume={50}, ISSN={0425-1644}, url={http://dx.doi.org/10.1111/evj.12734}, DOI={10.1111/evj.12734}, number={2}, journal={Equine Veterinary Journal}, publisher={Wiley}, author={Stewart, A. Stieler and Freund, J. M. and Gonzalez, L. M.}, year={2017}, month={Sep}, pages={241–248} }
 @article{stewart_pratt-phillips_gonzalez_2017, title={Alterations in Intestinal Permeability: The Role of the “Leaky Gut” in Health and Disease}, volume={52}, DOI={10.1016/j.jevs.2017.02.009}, journal={Journal of Equine Veterinary Science}, publisher={Elsevier BV}, author={Stewart, Amy Stieler and Pratt-Phillips, Shannon and Gonzalez, Liara M.}, year={2017}, month={May}, pages={10–22} }
 @article{kruger_gonzalez_pridgen_mccall_furstenberg_harnden_carnighan_cox_blikslager_garman_et al._2017, title={Ductular and proliferative response of esophageal submucosal glands in a porcine model of esophageal injury and repair}, volume={313}, DOI={10.1152/ajpgi.00036.2017}, number={3}, journal={American Journal of Physiology. Gastrointestinal and Liver Physiology}, author={Kruger, L. and Gonzalez, L. M. and Pridgen, T. A. and McCall, S. J. and Furstenberg, R. J. and Harnden, I. and Carnighan, G. E. and Cox, A. M. and Blikslager, A. T. and Garman, K. S. and et al.}, year={2017}, pages={G180–191} }
 @article{von furstenberg_li_stolarchuk_feder_campbell_kruger_gonzalez_blikslager_cardona_mccall_et al._2017, title={Porcine Esophageal Submucosal Gland Culture Model Shows Capacity for Proliferation and Differentiation}, volume={4}, ISSN={2352-345X}, url={http://dx.doi.org/10.1016/j.jcmgh.2017.07.005}, DOI={10.1016/j.jcmgh.2017.07.005}, number={3}, journal={Cellular and Molecular Gastroenterology and Hepatology}, publisher={Elsevier BV}, author={von Furstenberg, Richard J. and Li, Joy and Stolarchuk, Christina and Feder, Rachel and Campbell, Alexa and Kruger, Leandi and Gonzalez, Liara M. and Blikslager, Anthony T. and Cardona, Diana M. and McCall, Shannon J. and et al.}, year={2017}, month={Nov}, pages={385–404} }
 @article{simpson_gonzalez_chung_blikslager_magness_piedrahita_2016, title={27 AN IMPROVED LARGE ANIMAL MODEL FOR THE STUDY OF ADULT STEM CELLS}, volume={28}, ISSN={1031-3613}, url={http://dx.doi.org/10.1071/RDV28N2AB27}, DOI={10.1071/RDV28N2AB27}, abstractNote={

Murine models for the study of adult stem cell populations have broadened the understanding of previously uncharacterized stem cell niches. The development of murine reporter lines for the leucine-rich repeat-containing G-protein-coupled receptor-5 (Lgr5) has highlighted the importance of this gene as a stem cell marker in the stomach, intestine, hair follicle, liver, and kidney in mice. These models however have significant limitations in terms of translational applications because of anatomical and physiological differences between humans and mice. In order to overcome these limitations, we have sought to develop a porcine LGR5 reporter line. We report the generation of a porcine stem cell reporter line using the combination of transcription activator-like effector nucleases and somatic cell NT. Transcription activator-like effector nuclease-mediated homologous recombination was used to drive the integration of an internal ribosome entry site green fluorescent protein fusion into the 3′ untranslated region of the LGR5 locus in porcine fetal fibroblast cells. Multiple cell lines were developed and screened for the proper integration event. Upon confirmation of proper integration by genomic DNA sequencing, these lines were used as donors for somatic cell NT. Transfer of the somatic cell NT reconstructed embryos to a surrogate gilt resulted in 3 live births, and the establishment of a founder line of LGR5-green fluorescent protein reporter pigs. We have begun to characterise these lines, having observed fluorescent labelling of putative stem cell populations in the intestinal crypts and hair follicles from these animals. Many of these observations parallel the expression patterns observed in similar murine models. We have confirmed the fluorescent reporter signal by immunohistochemistry using an anti-green fluorescent protein antibody, and are working towards colocalization studies using anti-LGR5 antibodies and RNA in situ hybridization, as well as the characterisation of additional stem cell populations in the pig. The development of this line of transgenic pigs represents significant progress toward the study of adult stem cells, their progenitors, and the stem cell niche, using a large animal model with an anatomy, physiology, and ability to recapitulate human disease that overcomes the current limitations of rodent models.
Funding was provided by NIH R21OD019738.
}, number={2}, journal={Reproduction, Fertility and Development}, publisher={CSIRO Publishing}, author={Simpson, S. and Gonzalez, L. and Chung, J. and Blikslager, A. and Magness, S. and Piedrahita, J.}, year={2016}, pages={143} }
 @article{royal_gonzalez_2016, title={An Evaluation of the Psychometric Properties of an Advising Survey for Medical and Professional Program Students}, volume={6}, ISSN={1927-0534 1927-0526}, url={http://dx.doi.org/10.5539/jedp.v6n1p195}, DOI={10.5539/jedp.v6n1p195}, abstractNote={<p>The purpose of this study was to evaluate the psychometric properties of a newly developed instrument intended to measure faculty competence as it pertains to their role as advisors, particularly in medical and professional programs. A total of 166 students completed the Faculty Advisor’s Skills and Behaviors Inventory (FASBI). The psychometric properties of the FASBI were evaluated using the Rasch Rating Scale Model. Results indicate the FASBI is a psychometrically-sound instrument capable of producing valid and reproducible measures.</p>}, number={1}, journal={Journal of Educational and Developmental Psychology}, publisher={Canadian Center of Science and Education}, author={Royal, Kenneth D. and Gonzalez, Liara M.}, year={2016}, month={Apr}, pages={195} }
 @inbook{gonzalez_2016, place={Hoboken, NJ}, edition={3rd}, title={Intestinal Stem Cells}, booktitle={Equine Acute Abdomen}, publisher={Wiley & Sons, Inc}, author={Gonzalez, L.M.}, editor={Blikslager, A.T. and White, N.A. and Moore, J.N. and Mair, T.S.Editors}, year={2016} }
 @inbook{gonzalez_2016, place={Hoboken, NJ}, edition={3rd}, title={Intestinal Viability}, booktitle={Equine Acute Abdomen}, publisher={Wiley & Sons, Inc}, author={Gonzalez, L.M.}, editor={Blikslager, A.T. and White, N.A. and Moore, J.N. and Mair, T.S.Editors}, year={2016}, pages={570} }
 @article{ziegler_gonzalez_blikslager_2016, title={Large Animal Models: The Key to Translational Discovery in Digestive Disease Research}, volume={2}, ISSN={2352-345X}, url={http://dx.doi.org/10.1016/j.jcmgh.2016.09.003}, DOI={10.1016/j.jcmgh.2016.09.003}, number={6}, journal={Cellular and Molecular Gastroenterology and Hepatology}, publisher={Elsevier BV}, author={Ziegler, Amanda and Gonzalez, Liara and Blikslager, Anthony}, year={2016}, month={Nov}, pages={716–724} }
 @article{kruger_gonzalez_von furstenberg_henning_blikslager_garman_2016, title={Mo1253 Ductular and Proliferative Response of Esophageal Submucosal Glands in a Porcine Model of Esophageal Injury and Repair}, volume={150}, ISSN={0016-5085}, url={http://dx.doi.org/10.1016/S0016-5085(16)32312-5}, DOI={10.1016/S0016-5085(16)32312-5}, number={4}, journal={Gastroenterology}, publisher={Elsevier BV}, author={Kruger, Leandi and Gonzalez, Liara M. and von Furstenberg, Richard J. and Henning, Susan J. and Blikslager, Anthony and Garman, Katherine S.}, year={2016}, month={Apr}, pages={S679–S680} }
 @article{stewart_freund_magness_lund_blikslager_gonzalez_2016, title={Mo1298 Critical Contribution of Intestinal Stem Cells in the Repair of Ischemia Reperfusion Injury}, volume={150}, ISSN={0016-5085}, url={http://dx.doi.org/10.1016/S0016-5085(16)32355-1}, DOI={10.1016/S0016-5085(16)32355-1}, number={4}, journal={Gastroenterology}, publisher={Elsevier BV}, author={Stewart, Amy S. and Freund, John and Magness, Scott and Lund, Pauline K. and Blikslager, Anthony and Gonzalez, Liara M.}, year={2016}, month={Apr}, pages={S691} }
 @article{gonzalez_moeser_blikslager_2015, title={Animal models of ischemia-reperfusion-induced intestinal injury: Progress and promise for translational research}, volume={308}, DOI={10.1152/ajpgi.00112.2013}, number={2}, journal={American Journal of Physiology. Cell Physiology}, author={Gonzalez, Liara and Moeser, A. J. and Blikslager, Anthony}, year={2015}, pages={G63–75} }
 @article{gonzalez_kinnin_blikslager_2015, title={Characterization of discrete equine intestinal epithelial cell lineages}, volume={76}, DOI={10.2460/ajvr.76.4.358}, number={4}, journal={American Journal of Veterinary Research}, author={Gonzalez, Liara and Kinnin, L. A. and Blikslager, Anthony}, year={2015}, pages={358–366} }
 @article{gonzalez_fogle_baker_hughes_law_motsinger-reif_blikslager_2015, title={Operative factors associated with short-term outcome in horses with large colon volvulus: 47 cases from 2006 to 2013}, volume={47}, DOI={10.1111/evj.12273}, number={3}, journal={Equine Veterinary Journal}, author={Gonzalez, Liara and Fogle, Callie and Baker, W. T. and Hughes, F. E. and Law, J. M. and Motsinger-Reif, A. A. and Blikslager, Anthony}, year={2015}, pages={279–284} }
 @article{gonzalez_moeser_blikslager_2015, title={Porcine models of digestive disease: The future of large animal translational research}, volume={166}, DOI={10.1016/j.trsl.2015.01.004}, number={1}, journal={Translational Research}, author={Gonzalez, Liara and Moeser, A. J. and Blikslager, A. T.}, year={2015}, pages={12–27} }
 @article{gonzalez_2015, title={The mother of a gut cell: Intestinal epithelial stem cells}, volume={27}, DOI={10.1111/eve.12456}, number={11}, journal={Equine Veterinary Education}, author={Gonzalez, Liara}, year={2015}, pages={559–560} }
 @article{gonzalez_magness_lund_blikslager_2014, title={Mo1960 Intestinal Epithelial Cells Expressing Biomarkers of Crypt Base Columnar or Reserve Stem Cells Show Differential Resistance to Ischemia-Reperfusion Injury}, volume={146}, ISSN={0016-5085}, url={http://dx.doi.org/10.1016/S0016-5085(14)62550-6}, DOI={10.1016/S0016-5085(14)62550-6}, number={5}, journal={Gastroenterology}, publisher={Elsevier BV}, author={Gonzalez, Liara M. and Magness, Scott T. and Lund, Pauline K. and Blikslager, Anthony}, year={2014}, month={May}, pages={S-702} }
 @article{gonzalez_williamson_piedrahita_blikslager_magness_2013, title={Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration}, volume={8}, number={6}, journal={PLoS One}, author={Gonzalez, L. M. and Williamson, I. and Piedrahita, J. A. and Blikslager, A. T. and Magness, S. T.}, year={2013} }
 @article{gonzalez_blikslager_piedrahita_magness_2013, title={Su1099 A Translational Porcine Model of Intestinal Stem Cells}, volume={144}, ISSN={0016-5085}, url={http://dx.doi.org/10.1016/S0016-5085(13)61466-3}, DOI={10.1016/S0016-5085(13)61466-3}, number={5}, journal={Gastroenterology}, publisher={Elsevier BV}, author={Gonzalez, Liara and Blikslager, Anthony and Piedrahita, Jorge A. and Magness, Scott T.}, year={2013}, month={May}, pages={S-398} }
 @article{gonzalez_schramme_robertson_thrall_redding_2010, title={MRI FEATURES OF METACARPO(TARSO)PHALANGEAL REGION LAMENESS IN 40 HORSES}, volume={51}, ISSN={1058-8183 1740-8261}, url={http://dx.doi.org/10.1111/j.1740-8261.2010.01676.x}, DOI={10.1111/j.1740-8261.2010.01676.x}, number={4}, journal={Veterinary Radiology & Ultrasound}, publisher={Wiley}, author={Gonzalez, Liara M. and Schramme, Michael C. and Robertson, Ian D. and Thrall, Donald E. and Redding, Rich W.}, year={2010}, month={Apr}, pages={404–414} }