@article{recker_burton_cardinal_miranda_2013, title={Analysis of Quantitative Traits in Two Long-Term Randomly Mated Soybean Populations: I. Genetic Variances}, volume={53}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2012.10.0573}, abstractNote={ABSTRACTThe genetic effects of long‐term random mating and natural selection aided by genetic male sterility were evaluated in two soybean [Glycine max (L.) Merr.] populations: RSII and RSIII. Population means, variances, and heritabilities were estimated to determine the effects of 26 generations of random mating. The 10% highest yielding lines from each population were selected. Data was collected on flowering date, maturity date, plant height, lodging score, seed yield, seed weight, protein concentration (PC), and oil concentration (OC). RSII had a mean seed yield, PC, and OC of 2163 kg ha−1, 416 g kg−1, and 193 g kg−1, respectively. Entry‐mean heritability for seed yield, PC, and OC was estimated at 36, 68, and 57%, respectively. Theoretical response to selection was 183 kg ha−1 and, after selection, the realized gain was 161 kg ha−1. RSIII had a mean seed yield, PC, and OC of 2300 kg ha−1, 416 g kg−1, and 190 g kg−1, respectively. The entry‐mean heritability estimates for seed yield, PC, and OC were 57, 87, and 91%, respectively. Theoretical response to selection was 300 kg ha−1 and, after selection, realized gain was an increase of 139 kg ha−1. Despite not reaching their theoretical yield potential, the best lines from both populations compared well to check cultivars. The significant genetic variances and heritabilities observed demonstrate that the breakage of linkage blocks by long‐term random mating and natural selection were effective in producing competitive lines.}, number={4}, journal={CROP SCIENCE}, author={Recker, Jill R. and Burton, Joseph W. and Cardinal, Andrea and Miranda, Lilian}, year={2013}, pages={1375–1383} } @article{feng_burton_carter_miranda_st martin_brownie_2011, title={Genetic Analysis of Populations Derived from Matings of Southern and Northern Soybean Cultivars}, volume={51}, ISSN={["1435-0653"]}, DOI={10.2135/cropsci2010.12.0718}, abstractNote={ABSTRACTGenetic diversity is one of the important considerations in selecting parents for applied cultivar development in soybean [Glycine max (L.) Merr.]. Recent studies have shown that southern and northern U.S. soybean cultivars belong to two very distinct gene pools based on pedigree and molecular marker analysis. A study was conducted to evaluate the potential of matings between southern and northern cultivars for applied soybean breeding. Nine populations were derived from matings of two southern soybean cultivars, Johnston and Stonewall, with three ancestral, A.K. (Harrow), Lincoln, and Mandarin, and three improved, Flyer, Sibley, and Zane, northern cultivars. Field trials showed statistically significant differences among and within crosses for mean yield, maturity, height, seed quality, seed weight, protein, oil, and fatty acid composition. There were significant differences in means and genetic variances among populations derived from male (northern) cultivars for mean yield and all other traits recorded. Results suggested that improved northern cultivars would be useful parents for practical breeding programs in the south. Populations derived from Flyer produced the highest mean yields. A significant correlation (r = –0.82) between coefficient of parentage (CP) value and genetic variance estimate for cross yield may indicate that CP is a useful measurement in predicting genetic variation among matings of inbred soybean cultivars.}, number={6}, journal={CROP SCIENCE}, author={Feng, L. and Burton, J. W. and Carter, T. E., Jr. and Miranda, L. M. and St Martin, S. K. and Brownie, C.}, year={2011}, month={Nov}, pages={2479–2488} } @article{miranda_murphy_marshall_cowger_leath_2007, title={Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.)}, volume={114}, ISSN={0040-5752 1432-2242}, url={http://dx.doi.org/10.1007/s00122-007-0530-4}, DOI={10.1007/s00122-007-0530-4}, abstractNote={A single gene controlling powdery mildew resistance was identified in the North Carolina germplasm line NC96BGTD3 (NCD3) using genetic analysis of F(2) derived lines from a NCD3 X Saluda cross. Microsatellite markers linked to this Pm gene were identified and their most likely order was Xcfd7, 10.3 cM, Xgdm43, 8.6 cM, Xcfd26, 11.9 cM, Pm gene. These markers and the Pm gene were assigned to chromosome 5DL by means of Chinese Spring Nullitetrasomic (Nulli5D-tetra5A) and ditelosomic (Dt5DL) lines. A detached leaf test showed a distinctive disease reaction to six pathogen isolates among the NCD3 Pm gene, Pm2 (5DS) and Pm34 (5DL). An allelism test showed independence between Pm34 and the NCD3 Pm gene. Together, the tests provided strong evidence for the presence of a novel Pm gene in NCD3, and this gene was designated Pm35.}, number={8}, journal={Theoretical and Applied Genetics}, publisher={Springer Science and Business Media LLC}, author={Miranda, L. M. and Murphy, J. P. and Marshall, D. and Cowger, C. and Leath, S.}, year={2007}, month={Mar}, pages={1451–1456} } @article{miranda_perugini_srnic_brown-guedira_marshall_leath_murphy_2007, title={Genetic mapping of a Triticum monococcum-derived powdery mildew resistance gene in common wheat}, volume={47}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2007.01.0053}, abstractNote={Powdery mildew of wheat (Triticum aestivum L.) is a major fungal disease caused by Blumeria graminis DC f. sp. tritici A microsatellite linkage map was developed for the T. monococcum‐derived powdery mildew resistant gene present in the North Carolina germplasm line NCBGT96A6 (NCA6). Genetic analysis of F2‐derived lines from the cross NCA6 × ‘Saluda’ indicated a single gene controlled powdery mildew resistance. Four microsatellite markers linked to the NCA6 Pm gene mapped to chromosome 7AL. The most likely order was Xcfa2123‐0.9 cM–Xbarc121‐1.7 cM resistance gene/Xcfa2019‐3.0 cM‐Xgwm332 A detached‐leaf test indicated the disease reaction response of the NCA6 Pm gene was different from the five known alleles at the Pm1 locus on 7AL. Deletion interval mapping showed a large physical to genetic distance ratio for these microsatellite marker loci. This may be due to suppressed recombination between the introgressed T. monococcum segment and the homologous region of the T. aestivum cultivar Saluda. Our results suggested that the NCA6 Pm gene is likely a novel source of resistance to powdery mildew but additional allelism studies are needed to establish the relationship between this locus and the other known Pm loci on 7AL.}, number={6}, journal={CROP SCIENCE}, author={Miranda, L. M. and Perugini, L. and Srnic, G. and Brown-Guedira, G. and Marshall, D. and Leath, S. and Murphy, J. P.}, year={2007}, pages={2323–2329} } @article{miranda_murphy_marshall_leath_2006, title={Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.)}, volume={113}, ISSN={["1432-2242"]}, DOI={10.1007/s00122-006-0397-9}, abstractNote={Powdery mildew is a major fungal disease in wheat growing areas worldwide. A novel source of resistance to wheat powdery mildew present in the germplasm line NC97BGTD7 was genetically characterized as a monogenic trait in greenhouse and field trials using F(2) derived lines from a NC97BGTD7 X Saluda cross. Microsatellite markers were used to map and tag this resistance gene, now designated Pm34. Three co-dominant microsatellite markers linked to Pm34 were identified and their most likely order was established as: Xbarc177-5D, 5.4cM, Pm34, 2.6cM, Xbarc144-5D, 14cM, Xgwm272-5D. These microsatellite markers were previously mapped to the long arm of the 5D chromosome and their positions were confirmed using Chinese Spring nullitetrasomic Nulli5D-tetra5A and ditelosomic Dt5DL lines. Pm2, the only other known Pm gene on chromosome 5D, has been mapped to the short arm and its specificity is different from that of Pm34.}, number={8}, journal={THEORETICAL AND APPLIED GENETICS}, author={Miranda, L. M. and Murphy, J. P. and Marshall, D. and Leath, S.}, year={2006}, month={Nov}, pages={1497–1504} }