@article{staley_de los reyes_wang_barlaz_2018, title={Microbial ecological succession during municipal solid waste decomposition}, volume={102}, ISSN={0175-7598 1432-0614}, url={http://dx.doi.org/10.1007/s00253-018-9014-5}, DOI={10.1007/s00253-018-9014-5}, abstractNote={The decomposition of landfilled refuse proceeds through distinct phases, each defined by varying environmental factors such as volatile fatty acid concentration, pH, and substrate quality. The succession of microbial communities in response to these changing conditions was monitored in a laboratory-scale simulated landfill to minimize measurement difficulties experienced at field scale. 16S rRNA gene sequences retrieved at separate stages of decomposition showed significant succession in both Bacteria and methanogenic Archaea. A majority of Bacteria sequences in landfilled refuse belong to members of the phylum Firmicutes, while Proteobacteria levels fluctuated and Bacteroidetes levels increased as decomposition proceeded. Roughly 44% of archaeal sequences retrieved under conditions of low pH and high acetate were strictly hydrogenotrophic (Methanomicrobiales, Methanobacteriales). Methanosarcina was present at all stages of decomposition. Correspondence analysis showed bacterial population shifts were attributed to carboxylic acid concentration and solids hydrolysis, while archaeal populations were affected to a higher degree by pH. T-RFLP analysis showed specific taxonomic groups responded differently and exhibited unique responses during decomposition, suggesting that species composition and abundance within Bacteria and Archaea are highly dynamic. This study shows landfill microbial demographics are highly variable across both spatial and temporal transects.}, number={13}, journal={Applied Microbiology and Biotechnology}, publisher={Springer Science and Business Media LLC}, author={Staley, Bryan F. and de los Reyes, Francis L. and Wang, Ling and Barlaz, Morton A.}, year={2018}, month={Apr}, pages={5731–5740} }
 @article{de los reyes_weaver_wang_2015, title={A methodological framework for linking bioreactor function to microbial communities and environmental conditions}, volume={33}, ISSN={0958-1669}, url={http://dx.doi.org/10.1016/j.copbio.2015.02.002}, DOI={10.1016/j.copbio.2015.02.002}, abstractNote={In the continuing quest to relate microbial communities in bioreactors to function and environmental and operational conditions, engineers and biotechnologists have adopted the latest molecular and 'omic methods. Despite the large amounts of data generated, gaining mechanistic insights and using the data for predictive and practical purposes is still a huge challenge. We present a methodological framework that can guide experimental design, and discuss specific issues that can affect how researchers generate and use data to elucidate the relationships. We also identify, in general terms, bioreactor research opportunities that appear promising.}, journal={Current Opinion in Biotechnology}, publisher={Elsevier BV}, author={de los Reyes, Francis L, III and Weaver, Joseph E and Wang, Ling}, year={2015}, month={Jun}, pages={112–118} }
 @article{wang_aziz_reyes_2013, title={Determining the limits of anaerobic co-digestion of thickened waste activated sludge with grease interceptor waste}, volume={47}, ISSN={["0043-1354"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84878477665&partnerID=MN8TOARS}, DOI={10.1016/j.watres.2013.04.003}, abstractNote={Anaerobic co-digestion of thickened waste activated sludge (TWAS) with grease interceptor waste (GIW) from a food service establishment was conducted in lab scale semi-continuous digesters. GIW included the entire contents of the grease interceptor (GI) including fat, oil, and grease (FOG), food residuals, and associated wastewater. GIW was added in step increases to identify the maximum methane production and the corresponding threshold input of GIW that led to inhibition of methanogenesis. The experiment was performed at mesophilic conditions (37 °C) with a solids retention time (SRT) of 20 days. The highest GIW addition rate achieved without digester failure was 20% (v/v), or 65.5% (w/w) of volatile solids (VS) added, enhancing the methane yield from 0.180 to 0.752 mCH43/kgVSadded, biogas production from 2.2 × 10−3 to 1.4 × 10−2 m3/d, and methane content from 60.2% to 70.1%. The methane yield of 0.752 mCH43/kgVSadded is the highest value reported to date for co-digestion of GIW. Stepwise increases in co-substrate addition led to better microbial acclimation and reduced the GIW inhibitory effect. The limit for GIW addition leading to an inhibited digestion process was identified to be between 20 and 40% (v/v) or 65.5 and 83.5% (w/w) of VS added. The results show the significant benefits of anaerobic co-digestion of GIW and the positive impacts of gradual addition of GIW.}, number={11}, journal={WATER RESEARCH}, publisher={Elsevier BV}, author={Wang, Ling and Aziz, Tarek N. and Reyes, Francis L., III}, year={2013}, month={Jul}, pages={3835–3844} }