@article{jaiswal_yerke_bagley_ekelof_weber_haddad_fodor_muddiman_williams_2020, title={3D Imaging and metabolomic profiling reveal higher neuroactive kavalactone contents in lateral roots and crown root peels of Piper methysticum (kava)}, volume={9}, ISSN={["2047-217X"]}, DOI={10.1093/gigascience/giaa096}, abstractNote={Abstract
               
                  Background
                  Kava is an important neuroactive medicinal plant. While kava has a large global consumer footprint for its clinical and recreational use, factors related to its use lack standardization and the tissue-specific metabolite profile of its neuroactive constituents is not well understood.
               
               
                  Results
                  Here we characterized the metabolomic profile and spatio-temporal characteristics of tissues from the roots and stems using cross-platform metabolomics and a 3D imaging approach. Gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry revealed the highest content of kavalactones in crown root peels and lateral roots. Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) imaging revealed a unique tissue-specific presence of each target kavalactone. X-ray micro-computed tomography analysis demonstrated that lateral roots have morphological characteristics suitable for synthesis of the highest content of kavalactones.
               
               
                  Conclusions
                  These results provide mechanistic insights into the social and clinical practice of the use of only peeled roots by linking specific tissue characteristics to concentrations of neuroactive compounds.
               }, number={9}, journal={GIGASCIENCE}, author={Jaiswal, Yogini S. and Yerke, Aaron M. and Bagley, M. Caleb and Ekelof, Mans and Weber, Daniel and Haddad, Daniel and Fodor, Anthony and Muddiman, David C. and Williams, Leonard L.}, year={2020}, month={Sep} }
 @article{jaiswal_weber_yerke_xue_lehman_williams_xiao_haddad_williams_2019, title={A substitute variety for agronomically and medicinally important Serenoa repens (saw palmetto)}, volume={9}, ISSN={["2045-2322"]}, url={http://europepmc.org/abstract/med/30886216}, DOI={10.1038/s41598-019-41150-z}, abstractNote={AbstractSerenoa repens (saw palmetto) berries are one of the most consumed medicinal herbs in the United States and the wild green variety is used in the initial therapy of benign prostatic hyperplasia (BPH), globally. Use of saw palmetto is approved by the German Commission E, and several clinical trials are underway for evaluation of its efficacy. Exploitation of its habitats and over foraging imperil this plant, which only grows in the wild. This is the first study, to propose the use of the S. repens forma glauca (silver variety) as a qualitative substitute for the wild variety, to support its conservation. We compared tissue microstructures and lipid and water distribution through spatial imaging and examined metabolite distribution of three tissue domains and whole berries. This combined approach of 3D imaging and metabolomics provides a new strategy for studying phenotypic traits and metabolite synthesis of closely related plant varieties.}, number={1}, journal={SCIENTIFIC REPORTS}, author={Jaiswal, Yogini and Weber, Daniel and Yerke, Aaron and Xue, Yanling and Lehman, Danielle and Williams, Taufika and Xiao, Tiqiao and Haddad, Daniel and Williams, Leonard}, year={2019}, month={Mar} }
 @article{bansode_randolph_plundrich_lila_williams_2019, title={Peanut protein-polyphenol aggregate complexation suppresses allergic sensitization to peanut by reducing peanut-specific IgE in C3H/HeJ mice}, volume={299}, ISSN={["1873-7072"]}, DOI={10.1016/j.foodchem.2019.125025}, abstractNote={Peanut allergy is usually lifelong and accidental exposure impose formidable risk. The aim of this study was to assess the capacity of peanut proteins complexed to polyphenol extracts to reduce allergic response in C3H/HeJ mice. Mice were sensitized to peanut flour followed by exposure to amino acid diets fortified with peanut protein-polyphenol aggregates of either with low (15%; w/w) or high (40%; w/w) complexation ratios of blueberry (BB-Low and BB-High) and cranberry (CB-Low and CB-High) extracts. Treatment groups on diets with high complexation ratios of blueberry and cranberry aggregates showed significant reduction in peanut specific plasma Immunoglobulin E (IgE). Western blot analysis of spleen lysates showed CD63 protein expression was reduced in a dose-dependent manner in blueberry and cranberry complexed peanut protein supplemented diet groups. Our results demonstrate for the first time that complexation of polyphenols to peanut flour can potentially lower plasma IgE of peanut-sensitized C3H/HeJ mice.}, journal={FOOD CHEMISTRY}, author={Bansode, Rishipal R. and Randolph, Priscilla D. and Plundrich, Nathalie J. and Lila, Mary Ann and Williams, Leonard L.}, year={2019}, month={Nov} }
 @article{bansode_plundrich_randolph_lila_williams_2018, title={Peanut flour aggregation with polyphenolic extracts derived from peanut skin inhibits IgE binding capacity and attenuates RBL-2H3 cells degranulation via MAPK signaling pathway}, volume={263}, ISSN={["1873-7072"]}, DOI={10.1016/j.foodchem.2018.05.007}, abstractNote={This study investigates the anti-allergic properties of peanut skin polyphenols (PSP)-enriched peanut (PN) protein aggregates. PSP was blended with PN flour at concentrations of 5, 10, 15, 30, and 40% (w/w). Rat basophil leukemia cells (RBL-2H3) were sensitized with either anti-DNP-IgE or PN-allergic plasma followed by co-exposure to unmodified PN flour (control) or PSP-PN protein aggregates and Ca2+ ionophore, ionomycin. Immunoblotting and staining were performed to measure the IgE binding capacity of PSP-PN aggregates. Results showed that 30% PSP-PN aggregate significantly reduced β-hexosaminidase and histamine levels by 54.2% and 49.2%, respectively compared with control. Immunoblotting results revealed 40% PSP-PN aggregates significantly decreased IgE binding by 19%. The phosphorylation of p44/42 MAPK was significantly reduced while phosphorylation of p38 MAPK and SAPK/JNK increased upon PSP-PN protein aggregate exposure to the cells. Our results show that aggregation of PSP to PN proteins reduces allergic response by inhibiting Ca2+-induced MAPK-dependent cell degranulation.}, journal={FOOD CHEMISTRY}, author={Bansode, Rishipal R. and Plundrich, Nathalie J. and Randolph, Priscilla D. and Lila, Mary Ann and Williams, Leonard L.}, year={2018}, month={Oct}, pages={307–314} }