@article{probst_twiddy_hatada_pavlidis_daniele_sode_2024, title={Development of Direct Electron Transfer-Type Extended Gate Field Effect Transistor Enzymatic Sensors for Metabolite Detection}, volume={96}, ISSN={["1520-6882"]}, url={https://doi.org/10.1021/acs.analchem.3c04599}, DOI={10.1021/acs.analchem.3c04599}, abstractNote={In this work, direct electron transfer (DET)-type extended gate field effect transistor (EGFET) enzymatic sensors were developed by employing DET-type or quasi-DET-type enzymes to detect glucose or lactate in both 100 mM potassium phosphate buffer and artificial sweat. The system employed either a DET-type glucose dehydrogenase or a quasi-DET-type lactate oxidase, the latter of which was a mutant enzyme with suppressed oxidase activity and modified with amine-reactive phenazine ethosulfate. These enzymes were immobilized on the extended gate electrodes. Changes in the measured transistor drain current (ID) resulting from changes to the working electrode junction potential (φ) were observed as glucose and lactate concentrations were varied. Calibration curves were generated for both absolute measured ID and ΔID (normalized to a blank solution containing no substrate) to account for variations in enzyme immobilization and conjugation to the mediator and variations in reference electrode potential. This work resulted in a limit of detection of 53.9 μM (based on ID) for glucose and 2.12 mM (based on ID) for lactate, respectively. The DET-type and Quasi-DET-type EGFET enzymatic sensor was then modeled using the case of the lactate sensor as an equivalent circuit to validate the principle of sensor operation being driven through OCP changes caused by the substrate-enzyme interaction. The model showed slight deviation from collected empirical data with 7.3% error for the slope and 8.6% error for the y-intercept.}, number={10}, journal={ANALYTICAL CHEMISTRY}, author={Probst, David and Twiddy, Jack and Hatada, Mika and Pavlidis, Spyridon and Daniele, Michael and Sode, Koji}, year={2024}, month={Feb}, pages={4076–4085} }
 @article{turner_twiddy_wilkins_ramesh_kilgour_domingos_nasrallah_menegatti_daniele_2023, title={Biodegradable elastomeric circuit boards from citric acid-based polyesters}, volume={7}, ISSN={["2397-4621"]}, DOI={10.1038/s41528-023-00258-z}, abstractNote={Abstract}, number={1}, journal={NPJ FLEXIBLE ELECTRONICS}, author={Turner, Brendan L. and Twiddy, Jack and Wilkins, Michael D. and Ramesh, Srivatsan and Kilgour, Katie M. and Domingos, Eleo and Nasrallah, Olivia and Menegatti, Stefano and Daniele, Michael A.}, year={2023}, month={Jun} }
 @article{queener_ahmmed_victorio_twiddy_dehn_brewer_lobaton_bozkurt_pozdin_daniele_2023, place={Vienna, Austria}, title={Conformal Micropatterned Organic-Metal Electrodes for Physiological Recording}, ISSN={["1930-0395"]}, url={http://dx.doi.org/10.1109/sensors56945.2023.10324963}, DOI={10.1109/SENSORS56945.2023.10324963}, abstractNote={Conformal electrodes provide a soft and conforming interface with the skin for reduced impedance, comfortable skin contact, and improved signal quality compared to commercial electrodes. In this paper, we present conformal micropatterned organic-metal (CMOM) electrodes and our investigation on the effect of perforation micropatterning and PEDOT:PSS coating. CMOM electrodes were characterized then evaluated in vivo against commercial-off-the-shelf electrodes. PEDOT:PSS was found to reduce the overall impedance in each electrode variant, resulting in a >97% decrease in impedance at low frequencies. The change in impedance at high frequencies was not significant for the control or $30\ \mu \mathrm{m}$ vias electrodes, but the impedance was significantly greater following EPD for $60\ \mu \mathrm{m}$ vias electrodes.}, journal={2023 IEEE SENSORS}, author={Queener, Kirstie M. and Ahmmed, Parvez and Victorio, Mauro and Twiddy, Jack and Dehn, Ashley and Brewer, Alec and Lobaton, Edgar and Bozkurt, Alper and Pozdin, Vladimir and Daniele, Michael}, year={2023} }
 @article{rivera_bilton_burclaff_czerwinski_liu_trueblood_hinesley_breau_deal_joshi_et al._2023, title={Hypoxia Primes Human ISCs for Interleukin-Dependent Rescue of Stem Cell Activity}, volume={16}, ISSN={["2352-345X"]}, DOI={10.1016/j.jcmgh.2023.07.012}, abstractNote={Hypoxia in the intestinal epithelium can be caused by acute ischemic events or chronic inflammation in which immune cell infiltration produces inflammatory hypoxia starving the mucosa of oxygen. The epithelium has the capacity to regenerate after some ischemic and inflammatory conditions suggesting that intestinal stem cells (ISCs) are highly tolerant to acute and chronic hypoxia; however, the impact of hypoxia on human ISC (hISC) function has not been reported. Here we present a new microphysiological system (MPS) to investigate how hypoxia affects hISCs from healthy donors and test the hypothesis that prolonged hypoxia modulates how hISCs respond to inflammation-associated interleukins (ILs).hISCs were exposed to <1.0% oxygen in the MPS for 6, 24, 48, and 72 hours. Viability, hypoxia-inducible factor 1a (HIF1a) response, transcriptomics, cell cycle dynamics, and response to cytokines were evaluated in hISCs under hypoxia. HIF stabilizers and inhibitors were screened to evaluate HIF-dependent responses.The MPS enables precise, real-time control and monitoring of oxygen levels at the cell surface. Under hypoxia, hISCs maintain viability until 72 hours and exhibit peak HIF1a at 24 hours. hISC activity was reduced at 24 hours but recovered at 48 hours. Hypoxia induced increases in the proportion of hISCs in G1 and expression changes in 16 IL receptors. Prolyl hydroxylase inhibition failed to reproduce hypoxia-dependent IL-receptor expression patterns. hISC activity increased when treated IL1β, IL2, IL4, IL6, IL10, IL13, and IL25 and rescued hISC activity caused by 24 hours of hypoxia.Hypoxia pushes hISCs into a dormant but reversible proliferative state and primes hISCs to respond to a subset of ILs that preserves hISC activity. These findings have important implications for understanding intestinal epithelial regeneration mechanisms caused by inflammatory hypoxia.}, number={5}, journal={CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY}, author={Rivera, Kristina R. and Bilton, R. Jarrett and Burclaff, Joseph and Czerwinski, Michael J. and Liu, Jintong and Trueblood, Jessica M. and Hinesley, Caroline M. and Breau, Keith A. and Deal, Halston E. and Joshi, Shlok and et al.}, year={2023}, pages={823–846} }
 @article{yang_nithyanandam_kanetkar_kwon_ma_im_oh_shamsi_wilkins_daniele_et al._2023, title={Liquid Metal Coated Textiles with Autonomous Electrical Healing and Antibacterial Properties}, volume={4}, ISSN={["2365-709X"]}, DOI={10.1002/admt.202202183}, abstractNote={Abstract}, journal={ADVANCED MATERIALS TECHNOLOGIES}, author={Yang, Jiayi and Nithyanandam, Praneshnandan and Kanetkar, Shreyas and Kwon, Ki Yoon and Ma, Jinwoo and Im, Sooik and Oh, Ji-Hyun and Shamsi, Mohammad and Wilkins, Mike and Daniele, Michael and et al.}, year={2023}, month={Apr} }
 @article{prodromou_moore_chu_deal_san miguel_brown_daniele_pozdin_menegatti_2023, title={Molecular Engineering of Cyclic Azobenzene-Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo-Affinity Chromatography}, volume={1}, ISSN={["1616-3028"]}, url={http://dx.doi.org/10.1002/adfm.202213881}, DOI={10.1002/adfm.202213881}, abstractNote={Abstract}, journal={ADVANCED FUNCTIONAL MATERIALS}, publisher={Wiley}, author={Prodromou, Raphael and Moore, Brandyn David and Chu, Wenning and Deal, Halston and San Miguel, Adriana and Brown, Ashley Carson and Daniele, Michael Angelo-Anthony and Pozdin, Vladimir Aleksandrovich and Menegatti, Stefano}, year={2023}, month={Jan} }
 @article{kilgour_turner_daniele_menegatti_2023, title={One-Step Quantification of anti-Covid-19 Antibodies via Dual Affinity Ratiometric Quenching Assays}, volume={6}, ISSN={["1520-6882"]}, DOI={10.1021/acs.analchem.3c01266}, abstractNote={The global pandemic caused by acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people and paralyzed healthcare systems worldwide. Developing rapid and accurate tests to detect and quantify anti-SARS-CoV-2 antibodies in complex fluids is critical to (i) track and address the spread of SARS-CoV-2 variants with different virulence and (ii) support the industrial manufacturing and clinical administration of anti-SARS-CoV-2 therapeutic antibodies. Conventional immunoassays, such as lateral flow, ELISA, and surface plasmon resonance (SPR), are either qualitative or, when quantitative, are laborious and expensive and suffer from high variability. Responding to these challenges, this study evaluates the performance of the Dual-Affinity Ratiometric Quenching (DARQ) assay for the quantification of anti-SARS-CoV-2 antibodies in bioprocess harvests and intermediate fractions (i.e., a Chinese hamster ovary (CHO) cell culture supernatant and a purified eluate) and human fluids (i.e., saliva and plasma). Monoclonal antibodies targeting the SARS-CoV-2 nucleocapsid as well as the spike protein of the delta and omicron variants are adopted as model analytes. Additionally, conjugate pads loaded with dried protein were studied as an at-line quantification method that can be used in clinical or manufacturing laboratories. Our results indicate that the DARQ assay is a highly reproducible (coefficient of variation ∼0.5-3%) and rapid (<10 min) test, whose sensitivity (∼0.23-2.5 ng/mL), limit of detection (23-250 ng/mL), and dynamic range (70-1300 ng/mL) are independent of sample complexity, thus representing a valuable tool for monitoring anti-SARS-CoV-2 antibodies.}, journal={ANALYTICAL CHEMISTRY}, author={Kilgour, Katie M. and Turner, Brendan L. and Daniele, Michael and Menegatti, Stefano}, year={2023}, month={Jun} }
 @article{chu_shastry_barbieri_prodromou_greback-clarke_smith_moore_kilgore_cummings_pancorbo_et al._2023, title={Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates}, volume={7}, ISSN={["1097-0290"]}, DOI={10.1002/bit.28495}, abstractNote={Abstract}, journal={BIOTECHNOLOGY AND BIOENGINEERING}, author={Chu, Wenning and Shastry, Shriarjun and Barbieri, Eduardo and Prodromou, Raphael and Greback-Clarke, Paul and Smith, Will and Moore, Brandyn and Kilgore, Ryan and Cummings, Christopher and Pancorbo, Jennifer and et al.}, year={2023}, month={Jul} }
 @article{shastry_chu_barbieri_greback-clarke_smith_cummings_minzoni_pancorbo_gilleskie_ritola_et al._2023, title={Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography}, volume={9}, ISSN={["1860-7314"]}, DOI={10.1002/biot.202300230}, abstractNote={Abstract}, journal={BIOTECHNOLOGY JOURNAL}, author={Shastry, Shriarjun and Chu, Wenning and Barbieri, Eduardo and Greback-Clarke, Paul and Smith, William K. and Cummings, Christopher and Minzoni, Arianna and Pancorbo, Jennifer and Gilleskie, Gary and Ritola, Kimberly and et al.}, year={2023}, month={Sep} }
 @article{wang_hosseini_shastry_barbieri_chu_menegatti_daniele_2023, title={Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy}, DOI={10.1109/BioSensors58001.2023.10281105}, abstractNote={Gene therapies have shown great promise for the potential treatment of a broad range of diseases. Adeno-associated viruses (AAVs) are popular gene vectors because of their ability to target specific tissues, and they have demonstrated high transduction efficiencies in multiple neurological targets. While these therapeutics hold great promise, their biomanufacturing has limited potential cost-reduction and more widespread adoption. Herein, we report the preliminary development of an immunosensor for measuring the titer of adeno-associated virus 2 (AAV2), which may be deployed for rapid quantification of product yield during AAV biomanufacturing. We functionalized an interdigitated electrode array with anti-AAV2 antibodies, and electrochemical impedance spectroscopy was employed to investigate the response to AAV2 titer. A Faradaic sensing principle was utilized, in which the charge transfer resistance (Rct) of an electrochemical reporter was monitored after capture of AAV2 on the surface of the sensor. A linear response was measured over titers 1012 - 1013 capsids/mL.}, journal={2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS}, author={Wang, Junhyeong and Hosseini, Mahshid and Shastry, Shriarjun and Barbieri, Eduardo and Chu, Wenning and Menegatti, Stefano and Daniele, Michael A.}, year={2023} }
 @article{sharkey_twiddy_peterson_aroche_menegatti_daniele_2023, title={Towards electrochemical control of pH for regeneration of biosensors}, DOI={10.1109/BioSensors58001.2023.10281061}, abstractNote={Most affinity-based biosensors are designed to be single-use devices, based on the measurement of irreversible binding events, which makes longitudinal monitoring resource-intensive, and typically prohibits the measurement of analyte fluctuations over time using the same device. Selective reversal of biorecognition events, i.e., regeneration, may enable repeated and longitudinal use of affinity-based biosensors; however, typical regeneration methods utilize additional chemical reagents, requiring longer processing times and increasing the likelihood of operator error. The development of a “solid-state” regeneration method provides significant value for extending the utility of affinity-based biosensors, such as electrochemical immunosensors and aptasensors. Herein, we report the characterization of a method for electronically controlling pH without additional reagents. Palladium was used to induce pH swings in aqueous electrolytes and buffers by application of an electric potential. The developed system was able to affect acidic and basic pH changes of ± 4. The efficacy of this method was further demonstrated by reversing common affinity-binding complexes and compared to conventional glycine-based regeneration.}, journal={2023 IEEE BIOSENSORS CONFERENCE, BIOSENSORS}, author={Sharkey, Christopher and Twiddy, Jack and Peterson, Kaila L. and Aroche, Angelica F. and Menegatti, Stefano and Daniele, Michael A.}, year={2023} }
 @article{twiddy_peterson_maddocks_macpherson_pimentel_yates_armitano-lago_kiefer_pietrosimone_franz_et al._2022, title={A Low-Cost, Open Source Wireless Body Area Network for Clinical Gait Rehabilitation}, ISSN={["1930-0395"]}, DOI={10.1109/SENSORS52175.2022.9967362}, abstractNote={Wearable inertial sensors represent an opportunity to enable gait monitoring and feedback-based rehabilitation in real-world environments. Here, we describe the development of an inexpensive I MU-based wireless body area network capable of recording 9-axis motion data from 8 sites on the body simultaneously. This system can generate data comparable to existing commercial sensor networks and can distinguish varying loading conditions observed during real-time biofeedback-based human subject testing.}, journal={2022 IEEE SENSORS}, author={Twiddy, Jack and Peterson, Kaila and Maddocks, Grace and MacPherson, Ryan and Pimentel, Ricky and Yates, Max and Armitano-Lago, Cortney and Kiefer, Adam and Pietrosimone, Brian and Franz, Jason and et al.}, year={2022} }
 @article{suh_twiddy_mahmood_ali_lubna_bradford_daniele_gluck_2022, title={Electrospun Carbon Nanotube-Based Scaffolds Exhibit High Conductivity and Cytocompatibility for Tissue Engineering Applications}, volume={7}, ISSN={["2470-1343"]}, url={https://doi.org/10.1021/acsomega.2c01807}, DOI={10.1021/acsomega.2c01807}, abstractNote={Carbon nanotubes (CNTs) are known for their excellent conductive properties. Here, we present two novel methods, “sandwich” (sCNT) and dual deposition (DD CNT), for incorporating CNTs into electrospun polycaprolactone (PCL) and gelatin scaffolds to increase their conductance. Based on CNT percentage, the DD CNT scaffolds contain significantly higher quantities of CNTs than the sCNT scaffolds. The inclusion of CNTs increased the electrical conductance of scaffolds from 0.0 ± 0.00 kS (non-CNT) to 0.54 ± 0.10 kS (sCNT) and 5.22 ± 0.49 kS (DD CNT) when measured parallel to CNT arrays and to 0.25 ± 0.003 kS (sCNT) and 2.85 ± 1.12 (DD CNT) when measured orthogonally to CNT arrays. The inclusion of CNTs increased fiber diameter and pore size, promoting cellular migration into the scaffolds. CNT inclusion also decreased the degradation rate and increased hydrophobicity of scaffolds. Additionally, CNT inclusion increased Young’s modulus and failure load of scaffolds, increasing their mechanical robustness. Murine fibroblasts were maintained on the scaffolds for 30 days, demonstrating high cytocompatibility. The increased conductivity and high cytocompatibility of the CNT-incorporated scaffolds make them appropriate candidates for future use in cardiac and neural tissue engineering.}, number={23}, journal={ACS OMEGA}, publisher={American Chemical Society (ACS)}, author={Suh, Taylor C. and Twiddy, Jack and Mahmood, Nasif and Ali, Kiran M. and Lubna, Mostakima M. and Bradford, Philip D. and Daniele, Michael A. and Gluck, Jessica M.}, year={2022}, month={Jun}, pages={20006–20019} }
 @article{richmond_cole_dangler_daniele_marchitto_lobaton_2022, title={Forabot: Automated Planktic Foraminifera Isolation and Imaging}, volume={23}, ISSN={["1525-2027"]}, url={http://dx.doi.org/10.1029/2022gc010689}, DOI={10.1029/2022GC010689}, abstractNote={Abstract}, number={12}, journal={GEOCHEMISTRY GEOPHYSICS GEOSYSTEMS}, publisher={American Geophysical Union (AGU)}, author={Richmond, Turner and Cole, Jeremy and Dangler, Gabriella and Daniele, Michael and Marchitto, Thomas and Lobaton, Edgar}, year={2022}, month={Dec} }
 @article{twiddy_taggart_reynolds_sharkey_rufty_lobaton_bozkurt_daniele_2022, title={Real-Time Monitoring of Plant Stalk Growth Using a Flexible Printed Circuit Board Sensor}, ISSN={["1930-0395"]}, DOI={10.1109/SENSORS52175.2022.9967167}, abstractNote={Monitoring of plant growth within agriculture is essential for ensuring the survival of crops and optimization of resources in the face of environmental and industrial challenges. Herein, we describe a low-cost and easily deployable flexible circuit board sensor for measurement of plant stalk growth, providing for remote tracking of plant development on an industrial scale. Three circuit topologies and measurement strategies - “ladder-type,” “multiplex-type,” and “mixed-type” - are initially assessed off-plant in a simulated growth experiment. Further development of the “multiplex-type” sensor and on-plant validation demonstrates its ability to quantify stalk growth as a proxy for plant development.}, journal={2022 IEEE SENSORS}, author={Twiddy, Jack and Taggart, Matthew and Reynolds, James and Sharkey, Chris and Rufty, Thomas and Lobaton, Edgar and Bozkurt, Alper and Daniele, Michael}, year={2022} }
 @article{lee_aroche_menegatti_daniele_2022, title={Toward an Aptasensor for Monitoring of Tacrolimus}, ISSN={["1930-0395"]}, DOI={10.1109/SENSORS52175.2022.9967265}, abstractNote={Developing a simple and accurate method for monitoring the levels of circulating tacrolimus (TAC) can inform dosing schedule and reduce the risk of rejection in solid organ transplantation. Thus, an aptasensor was designed, fabricated, and characterized for electrochemical quantification of TAC concentration. The TAC-aptasensor comprised a gold electrode functionalized with a thiolated TAC-binding aptamer (APT122) derivatized with methylene blue. Surface plasmon resonance (SPR) was used to characterize the binding strength and specificity of the TAC:APT122 complex. Square wave voltammetry (SWV) was used to quantify the aptasensor response to varying concentrations of TAC. A dose-response curve was observed at concentrations of TAC < 7 µM, when operated at 50 and 80 Hz. Typical sensitivity of 0.07 µA· µM-1 was observed at 50 Hz. Limited specificity for the TAC aptasensors was observed with SWV against cyclosporine, amoxicillin, and tetracycline. Future efforts will explore a multi-aptamer system to extend the dynamic range and improve surface functionality to limit non-specific binding.}, journal={2022 IEEE SENSORS}, author={Lee, Bang Hyun and Aroche, Angelica F. and Menegatti, Stefano and Daniele, Michael A.}, year={2022} }
 @article{saha_songkakul_knisely_yokus_daniele_dickey_bozkurt_velev_2022, title={Wireless Wearable Electrochemical Sensing Platform with Zero- Power Osmotic Sweat Extraction for Continuous Lactate Monitoring}, volume={7}, ISSN={["2379-3694"]}, url={https://doi.org/10.1021/acssensors.2c00830}, DOI={10.1021/acssensors.2c00830}, abstractNote={Wearable and wireless monitoring of biomarkers such as lactate in sweat can provide a deeper understanding of a subject's metabolic stressors, cardiovascular health, and physiological response to exercise. However, the state-of-the-art wearable and wireless electrochemical systems rely on active sweat released either via high-exertion exercise, electrical stimulation (such as iontophoresis requiring electrical power), or chemical stimulation (such as by delivering pilocarpine or carbachol inside skin), to extract sweat under low-perspiring conditions such as at rest. Here, we present a continuous sweat lactate monitoring platform combining a hydrogel for osmotic sweat extraction, with a paper microfluidic channel for facilitating sweat transport and management, a screen-printed electrochemical lactate sensor, and a custom-built wireless wearable potentiostat system. Osmosis enables zero-electrical power sweat extraction at rest, while continuous evaporation at the end of a paper channel allows long-term sensing from fresh sweat. The positioning of the lactate sensors provides near-instantaneous sensing at low sweat volume, and the custom-designed potentiostat supports continuous monitoring with ultra-low power consumption. For a proof of concept, the prototype system was evaluated for continuous measurement of sweat lactate across a range of physiological activities with changing lactate concentrations and sweat rates: for 2 h at the resting state, 1 h during medium-intensity exercise, and 30 min during high-intensity exercise. Overall, this wearable system holds the potential of providing comprehensive and long-term continuous analysis of sweat lactate trends in the human body during rest and under exercising conditions.}, journal={ACS SENSORS}, publisher={American Chemical Society (ACS)}, author={Saha, Tamoghna and Songkakul, Tanner and Knisely, Charles T. and Yokus, Murat A. and Daniele, Michael A. and Dickey, Michael D. and Bozkurt, Alper and Velev, Orlin D.}, year={2022}, month={Jul} }
 @article{saha_fang_yokus_mukherjee_bozkurt_daniele_dickey_velev_2021, title={A Wearable Patch for Prolonged Sweat Lactate Harvesting and Sensing}, ISSN={["1558-4615"]}, DOI={10.1109/EMBC46164.2021.9630881}, abstractNote={Operating at low sweat rates, such as those experienced by humans at rest, is still an unmet need for state-of-the-art wearable sweat harvesting and testing devices for lactate. Here, we report the on-skin performance of a non-invasive wearable sweat sampling patch that can harvest sweat at rest, during exercise, and post-exercise. The patch simultaneously uses osmosis and evaporation for long-term (several hours) sampling of sweat. Osmotic sweat withdrawal is achieved by skin-interfacing a hydrogel containing a concentrated solute. The gel interfaces with a paper strip that transports the fluid via wicking and evaporation. Proof of concept results show that the patch was able to sample sweat during resting and post-exercise conditions, where the lactate concentration was successfully quantified. The patch detected the increase in sweat lactate levels during medium level exercise. Blood lactate remained invariant with exercise as expected. We also developed a continuous sensing version of the patch by including enzymatic electrochemical sensors. Such a battery-free, passive, wearable sweat sampling patch can potentially provide useful information about the human metabolic activity.}, journal={2021 43RD ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE & BIOLOGY SOCIETY (EMBC)}, author={Saha, Tamoghna and Fang, Jennifer and Yokus, Murat A. and Mukherjee, Sneha and Bozkurt, Alper and Daniele, Michael A. and Dickey, Michael D. and Velev, Orlin D.}, year={2021}, pages={6863–6866} }
 @article{prodromou_day_saberi-bosari_schneible_mabe_san miguel_daniele_pozdin_menegatti_2021, title={Engineering Next Generation Cyclized Peptide Ligands for Light-Controlled Capture and Release of Therapeutic Proteins}, volume={31}, ISSN={["1616-3028"]}, url={http://dx.doi.org/10.1002/adfm.202101410}, DOI={10.1002/adfm.202101410}, abstractNote={Abstract}, number={27}, journal={ADVANCED FUNCTIONAL MATERIALS}, publisher={Wiley}, author={Prodromou, Raphael and Day, Kevin N. and Saberi-Bosari, Sahand and Schneible, John D. and Mabe, Matthew D. and San Miguel, Adriana and Daniele, Michael A. and Pozdin, Vladimir and Menegatti, Stefano}, year={2021}, month={Jul} }
 @article{mihalko_sandry_mininni_nellenbach_deal_daniele_ghadimi_levy_brown_2021, title={Fibrin-modulating nanogels for treatment of disseminated intravascular coagulation}, volume={5}, ISSN={["2473-9537"]}, DOI={10.1182/bloodadvances.2020003046}, abstractNote={Abstract}, number={3}, journal={BLOOD ADVANCES}, author={Mihalko, Emily P. and Sandry, Megan and Mininni, Nicholas and Nellenbach, Kimberly and Deal, Halston and Daniele, Michael and Ghadimi, Kamrouz and Levy, Jerrold H. and Brown, Ashley C.}, year={2021}, month={Feb}, pages={613–627} }
 @article{yokus_daniele_2021, title={Integrated non-invasive biochemical and biophysical sensing systems for health and performance monitoring: A systems perspective}, volume={184}, ISSN={["1873-4235"]}, DOI={10.1016/j.bios.2021.113249}, abstractNote={Advances in materials, bio-recognition elements, transducers, and microfabrication techniques, as well as progress in electronics, signal processing, and wireless communication have generated a new class of skin-interfaced wearable health monitoring systems for applications in personalized medicine and digital health. In comparison to conventional medical devices, these wearable systems are at the cusp of initiating a new era of longitudinal and noninvasive sensing for the prevention, detection, diagnosis, and treatment of diseases at the molecular level. Herein, we provide a review of recent developments in wearable biochemical and biophysical systems. We survey the sweat sampling and collection methods for biochemical systems, followed by an assessment of biochemical and biophysical sensors deployed in current wearable systems with an emphasis on their hardware specifications. Specifically, we address how sweat collection and sample handling platforms may be a rate limiting technology to realizing the clinical translation of wearable health monitoring systems; moreover, we highlight the importance of achieving both longitudinal sensing and assessment of intrapersonal variation in sweat-blood correlations to have the greatest clinical impact. Lastly, we assess a snapshot of integrated wireless wearable systems with multimodal sensing capabilities, and we conclude with our perspective on the state-of-the-art and the required developments to achieve the next-generation of integrated wearable health and performance monitoring systems.}, journal={BIOSENSORS & BIOELECTRONICS}, author={Yokus, Murat A. and Daniele, Michael A.}, year={2021}, month={Jul} }
 @article{martins_wilkins_ligler_daniele_freytes_2021, title={Microphysiological System for High-Throughput Computer Vision Measurement of Microtissue Contraction}, volume={6}, ISSN={["2379-3694"]}, DOI={10.1021/acssensors.0c02172}, abstractNote={The ability to measure microtissue contraction in vitro can provide important information when modeling cardiac, cardiovascular, respiratory, digestive, dermal, and skeletal tissues. However, measuring tissue contraction in vitro often requires the use of high number of cells per tissue construct along with time-consuming microscopy and image analysis. Here, we present an inexpensive, versatile, high-throughput platform to measure microtissue contraction in a 96-well plate configuration using one-step batch imaging. More specifically, optical fiber microprobes are embedded in microtissues, and contraction is measured as a function of the deflection of optical signals emitted from the end of the fibers. Signals can be measured from all the filled wells on the plate simultaneously using a digital camera. An algorithm uses pixel-based image analysis and computer vision techniques for the accurate multiwell quantification of positional changes in the optical microprobes caused by the contraction of the microtissues. Microtissue constructs containing 20,000-100,000 human ventricular cardiac fibroblasts (NHCF-V) in 6 mg/mL collagen type I showed contractile displacements ranging from 20-200 μm. This highly sensitive and versatile platform can be used for the high-throughput screening of microtissues in disease modeling, drug screening for therapeutics, physiology research, and safety pharmacology.}, number={3}, journal={ACS SENSORS}, author={Martins, Ana Maria Gracioso and Wilkins, Michael D. and Ligler, Frances S. and Daniele, Michael A. and Freytes, Donald O.}, year={2021}, month={Mar}, pages={985–994} }
 @article{pozdin_erb_downey_rivera_daniele_2021, title={Monitoring of random microvessel network formation by in-line sensing of flow rates: A numerical and in vitro investigation}, volume={331}, ISSN={["1873-3069"]}, DOI={10.1016/j.sna.2021.112970}, abstractNote={The directed or de novo formation of microvasculature in engineered tissue constructs is essential for accurately replicating physiological function. A limiting factor of a system relying on spontaneous microvessel formation is the inability to precisely quantify the development of the microvascular network and control fluid moving through formed vessels. Herein, we report a strategy to monitor the dynamic formation of microscale fluid networks, which can be translated to the monitoring of microvasculature development in engineered tissue constructs. The non-invasive, non-destructive monitoring and characterization of the fluid network is achieved via in-line sensing of fluid flow rate and correlating this measurement to the hydrodynamic resistance of the fluid network to model the progression of microvessel formation and connectivity. Computational fluid dynamics, equivalent circuit, and experimental models were compared, which simulated multi-generational branching or splitting microvessel networks. The networks simulated vessels with varying cross-sectional area, up to 16 branching vessels, and microvessel network volume ranging from ˜20−30 mm3. In all models, the increasing degree of network complexity and volume corresponded to a decrease in jumper flow-rate measured; however, vessel cross-section also impacted the measured jumper flow rate, i.e. at low vessel height (<200 μm) response was dominated by increased network volume and at higher vessel height (>200 μm) the response was dominated by resistance of narrow channels. An approximately 2% error was exhibited between the models, which was attributed to variation in the geometry of the fabricated models and illustrates the potential to precisely and non-destructively monitor microvessel network development and volumetric changes.}, journal={SENSORS AND ACTUATORS A-PHYSICAL}, author={Pozdin, Vladimir A. and Erb, Patrick D. and Downey, McKenna and Rivera, Kristina R. and Daniele, Michael}, year={2021}, month={Nov} }
 @article{songkakul_peterson_daniele_bozkurt_2021, title={Preliminary Evaluation of a Solar-Powered Wristband for Continuous Multi-Modal Electrochemical Monitoring}, ISSN={["1558-4615"]}, DOI={10.1109/EMBC46164.2021.9630105}, abstractNote={Continuous, non-invasive wearable measurement of metabolic biomarkers could provide vital insight into patient condition for personalized health and wellness monitoring. We present our efforts towards developing a wearable solar-powered electrochemical platform for multimodal sweat based metabolic monitoring. This wrist-worn wearable system consists of a flexible photovoltaic cell connected to a circuit board containing ultra low power circuitry for sensor data collection, energy harvesting, and wireless data transmission, all integrated into an elastic fabric wristband. The system continuously samples amperometric, potentiometric, temperature, and motion data and wirelessly transmits these to a data aggregator. The full wearable system is 7.5 cm long and 5 cm in diameter, weighs 22 grams, and can run directly from harvested light energy. Relatively low levels of light such as residential lighting (∼200 lux) are sufficient for continuous operation of the system. Excess harvested energy is stored in a small 37 mWh lithium polymer battery. The battery can be charged in ∼14 minutes under full sunlight and can power the system for ∼8 days when fully charged. The system has an average power consumption of 176 µW. The solar-harvesting performance of the system was characterized in a variety of lighting conditions, and the amperometric and potentiometric electrochemical capabilities of the system were validated in vitro.Clinical relevance—The presented solar-powered wearable system enables continuous wireless multi-modal electrochemical monitoring for uninterrupted sensing of metabolic biomarkers in sweat while harvesting energy from indoor lighting or sunlight.}, journal={2021 43RD ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE & BIOLOGY SOCIETY (EMBC)}, author={Songkakul, Tanner and Peterson, Kaila and Daniele, Michael and Bozkurt, Alper}, year={2021}, pages={7316–7319} }
 @article{hiraka_tsugawa_asano_yokus_ikebukuro_daniele_sode_2021, title={Rational design of direct electron transfer type L-lactate dehydrogenase for the development of multiplexed biosensor}, volume={176}, ISSN={["1873-4235"]}, DOI={10.1016/j.bios.2020.112933}, abstractNote={The development of wearable multiplexed biosensors has been focused on systems to measure sweat l-lactate and other metabolites, where the employment of the direct electron transfer (DET) principle is expected. In this paper, a fusion enzyme between an engineered l-lactate oxidase derived from Aerococcus viridans, AvLOx A96L/N212K mutant, which is minimized its oxidase activity and b-type cytochrome protein was constructed to realize multiplexed DET-type lactate and glucose sensors. The sensor with a fusion enzyme showed DET to a gold electrode, with a limited operational range less than 0.5 mM. A mutation was introduced into the fusion enzyme to increase Km value and eliminate its substrate inhibition to construct “b2LOxS”. Together with the employment of an outer membrane, the detection range of the sensor with b2LOxS was expanded up to 10 mM. A simultaneous lactate and glucose monitoring system was constructed using a flexible thin-film multiplexed electrodes with b2LOxS and a DET-type glucose dehydrogenase, and evaluated their performance in the artificial sweat. The sensors achieved simultaneous detection of lactate and glucose without cross-talking error, with the detected linear ranges of 0.5–20 mM for lactate and 0.1–5 mM for glucose, sensitivities of 4.1 nA/mM∙mm2 for lactate and 56 nA/mM∙mm2 for glucose, and limit of detections of 0.41 mM for lactate and 0.057 mM for glucose. The impact of the presence of electrochemical interferants (ascorbic acid, acetaminophen and uric acid), was revealed to be negligible. This is the first report of the DET-type enzyme based lactate and glucose dual sensing systems.}, journal={BIOSENSORS & BIOELECTRONICS}, author={Hiraka, Kentaro and Tsugawa, Wakako and Asano, Ryutaro and Yokus, Murat A. and Ikebukuro, Kazunori and Daniele, Michael A. and Sode, Koji}, year={2021}, month={Mar} }
 @article{turner_ramesh_menegatti_daniele_2021, title={Resorbable elastomers for implantable medical devices: highlights and applications}, volume={12}, ISSN={["1097-0126"]}, DOI={10.1002/pi.6349}, abstractNote={Abstract}, journal={POLYMER INTERNATIONAL}, author={Turner, Brendan and Ramesh, Srivatsan and Menegatti, Stefano and Daniele, Michael}, year={2021}, month={Dec} }
 @article{roosa_muhamed_young_nellenbach_daniele_ligler_brown_2021, title={Synthesis of sonicated fibrin nanoparticles that modulate fibrin clot polymerization and enhance angiogenic responses}, volume={204}, ISSN={["1873-4367"]}, DOI={10.1016/j.colsurfb.2021.111805}, abstractNote={Chronic wounds can occur when the healing process is disrupted and the wound remains in a prolonged inflammatory stage that leads to severe tissue damage and poor healing outcomes. Clinically used treatments, such as high density, FDA-approved fibrin sealants, do not provide an optimal environment for native cell proliferation and subsequent tissue regeneration. Therefore, new treatments outside the confines of these conventional fibrin bulk gel therapies are required. We have previously developed flowable, low-density fibrin nanoparticles that, when coupled to keratinocyte growth factor, promote cell migration and epithelial wound closure in vivo. Here, we report a new high throughput method for generating the fibrin nanoparticles using probe sonication, which is less time intensive than the previously reported microfluidic method, and investigate the ability of the sonicated fibrin nanoparticles (SFBN) to promote clot formation and cell migration in vitro. The SFBNs can form a fibrin gel when combined with fibrinogen in the absence of exogenous thrombin, and the polymerization rate and fiber density in these fibrin clots is tunable based on SFBN concentration. Furthermore, fibrin gels made with SFBNs support cell migration in an in vitro angiogenic sprouting assay, which is relevant for wound healing. In this report, we show that SFBNs may be a promising wound healing therapy that can be easily produced and delivered in a flowable formulation.}, journal={COLLOIDS AND SURFACES B-BIOINTERFACES}, author={Roosa, Colleen A. and Muhamed, Ismaeel and Young, Ashlyn T. and Nellenbach, Kimberly and Daniele, Michael A. and Ligler, Frances S. and Brown, Ashley C.}, year={2021}, month={Aug} }
 @article{nandi_mihalko_nellenbach_castaneda_schneible_harp_deal_daniele_menegatti_barker_et al._2021, title={Synthetic Platelet Microgels Containing Fibrin Knob B Mimetic Motifs Enhance Clotting Responses}, volume={4}, ISSN={["2366-3987"]}, DOI={10.1002/adtp.202100010}, abstractNote={Abstract}, number={5}, journal={ADVANCED THERAPEUTICS}, author={Nandi, Seema and Mihalko, Emily and Nellenbach, Kimberly and Castaneda, Mario and Schneible, John and Harp, Mary and Deal, Halston and Daniele, Michael and Menegatti, Stefano and Barker, Thomas H. and et al.}, year={2021}, month={May} }
 @article{chen_wilkins_barahona_rosenbaum_daniele_lobaton_2021, title={Toward Automated Analysis of Fetal Phonocardiograms: Comparing Heartbeat Detection from Fetal Doppler and Digital Stethoscope Signals}, ISSN={["1558-4615"]}, url={http://dx.doi.org/10.1109/embc46164.2021.9629814}, DOI={10.1109/EMBC46164.2021.9629814}, abstractNote={Longitudinal fetal health monitoring is essential for high-risk pregnancies. Heart rate and heart rate variability are prime indicators of fetal health. In this work, we implemented two neural network architectures for heartbeat detection on a set of fetal phonocardiogram signals captured using fetal Doppler and a digital stethoscope. We test the efficacy of these networks using the raw signals and the hand-crafted energy from the signal. The results show a Convolutional Neural Network is the most efficient at identifying the S1 waveforms in a heartbeat, and its performance is improved when using the energy of the Doppler signals. We further discuss issues, such as low Signal-to-Noise Ratios (SNR), present in the training of a model based on the stethoscope signals. Finally, we show that we can improve the SNR, and subsequently the performance of the stethoscope, by matching the energy from the stethoscope to that of the Doppler signal.}, journal={2021 43rd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)}, publisher={IEEE}, author={Chen, Yuhan and Wilkins, Michael D. and Barahona, Jeffrey and Rosenbaum, Alan J. and Daniele, Michael and Lobaton, Edgar}, year={2021}, pages={975–979} }
 @article{richardson_maddocks_peterson_daniele_pavlidis_2021, title={Toward Subcutaneous Electrochemical Aptasensors for Neuropeptide Y}, ISSN={["1930-0395"]}, DOI={10.1109/SENSORS47087.2021.9639832}, abstractNote={Subcutaneous sensors, similar to the continuous glucose monitor, are advantageous for identifying healthy and pathological patterns of circulating biomarkers. A biosensor for the detection of neuropeptide Y (NPY), a marker of stress, has been designed and tested for operation in a flexible microneedle form factor. The biosensing principle used is affinity binding of NPY to a DNA aptamer-functionalized electrode. A gold microelectrode was functionalized by formation of a self- assembled monolayer (SAM) of a thiol-modified NPY-binding aptamer and poly(ethylene glycol) methyl ether thiol (PEG). The sensors were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy, resulting in a response to NPY over 400 pM to 200 nM when tested in KCl and K3[Fe(CN)6]/K4[Fe(CN)6], and PBS.}, journal={2021 IEEE SENSORS}, author={Richardson, Hayley and Maddocks, Grace and Peterson, Kaila and Daniele, Michael and Pavlidis, Spyridon}, year={2021} }
 @article{martin_reynolds_daniele_lobaton_bozkurt_2021, title={Towards Continuous Plant Bioimpedance Fitting and Parameter Estimation}, ISSN={["1930-0395"]}, url={http://dx.doi.org/10.1109/sensors47087.2021.9639492}, DOI={10.1109/SENSORS47087.2021.9639492}, abstractNote={The push to advance artificial intelligence, internet of things, and big data analysis all pave the way to automated and systematic optimization in precision agriculture and smart farming applications. These advancements lead to many benefits, including the optimization of primary production, prevention of spoilage via supply chain management, and detection of crop failure risk. Noninvasive impedance sensors serve as a promising candidate for monitoring plant health wirelessly and play a major role in this optimization problem. In this study, we developed a software pipeline to support impedance sensing applications and, as a proof of concept, applied this to track longitudinal consistent bioimpedance data from the V4 leaf midrib in maize plants. The script uses the single-shell equivalent circuit model to represent the extracellular fluid, cellular membrane, and intracellular fluid as a simplified resistive-capacitive circuit, where these elements’ parameters are estimated with complex nonlinear least squares. The double-shell model extends the single-shell model to account for the effects of the relatively large plant cell vacuole. Limit cases for impedance are utilized for specific parameters as an alternative method of estimation. We investigated a complex analysis-based modification to the objective function and model optimization for the data pipeline automation. Various weighing functions are applied and checked against one another. Additionally, a custom graphical user interface was developed to assist with parameter initialization for correcting potential convergence issues and understating the influence of each parameter on the dataset. We demonstrated that the analysis of an example longitudinal dataset was able to reveal a time series for parameter fitting.}, journal={2021 IEEE SENSORS}, publisher={IEEE}, author={Martin, Devon and Reynolds, James and Daniele, Michael and Lobaton, Edgar and Bozkurt, Alper}, year={2021} }
 @article{turner_senevirathne_kilgour_mcart_biggs_menegatti_daniele_2021, title={Ultrasound-Powered Implants: A Critical Review of Piezoelectric Material Selection and Applications}, volume={7}, ISSN={["2192-2659"]}, DOI={10.1002/adhm.202100986}, abstractNote={Abstract}, journal={ADVANCED HEALTHCARE MATERIALS}, author={Turner, Brendan L. and Senevirathne, Seedevi and Kilgour, Katie and McArt, Darragh and Biggs, Manus and Menegatti, Stefano and Daniele, Michael A.}, year={2021}, month={Jul} }
 @article{su_huang_mathews_scharf_hu_li_frame_cores_dinh_daniele_et al._2020, title={Cardiac Stromal Cell Patch Integrated with Engineered Microvessels Improves Recovery from Myocardial Infarction in Rats and Pigs}, volume={6}, ISSN={["2373-9878"]}, DOI={10.1021/acsbiomaterials.0c00942}, abstractNote={The vascularized cardiac patch strategy is promising for ischemic heart repair after myocardial infarction (MI), but current fabrication processes are quite complicated. Vascularized cardiac patches that can promote concurrent restoration of both the myocardium and vasculature at the injured site in a large animal model remain elusive. The safety and therapeutic benefits of a cardiac stromal cell patch integrated with engineered biomimetic microvessels (BMVs) were determined for treating MI. By leveraging a microfluidic method employing hydrodynamic focusing, we constructed the endothelialized microvessels and then encapsulated them together with therapeutic cardiosphere-derived stromal cells (CSCs) in a fibrin gel to generate a prevascularized cardiac stromal cell patch (BMV-CSC patch). We showed that BMV-CSC patch transplantation significantly promoted cardiac function, reduced scar size, increased viable myocardial tissue, promoted neovascularization, and suppressed inflammation in rat and porcine MI models, demonstrating enhanced therapeutic efficacy compared to conventional cardiac stromal cell patches. BMV-CSC patches did not increase renal and hepatic toxicity or exhibit immunogenicity. We noted a significant increase in endogenous progenitor cell recruitment to the peri-infarct region of the porcine hearts treated with BMV-CSC patch as compared to those that received control treatments. These findings establish the BMV-CSC patch as a novel engineered-tissue therapeutic for ischemic tissue repair.}, number={11}, journal={ACS BIOMATERIALS SCIENCE & ENGINEERING}, author={Su, Teng and Huang, Ke and Mathews, Kyle G. and Scharf, Valery F. and Hu, Shiqi and Li, Zhenhua and Frame, Brianna N. and Cores, Jhon and Dinh, Phuong-Uyen and Daniele, Michael A. and et al.}, year={2020}, month={Nov}, pages={6309–6320} }
 @article{schneible_young_daniele_menegatti_2020, title={Chitosan Hydrogels for Synergistic Delivery of Chemotherapeutics to Triple Negative Breast Cancer Cells and Spheroids}, volume={37}, ISBN={1573-904X}, DOI={10.1007/s11095-020-02864-2}, abstractNote={This study aimed to develop a hydrogel system for treating aggressive triple negative breast cancer (TNBC) via kinetically-controlled delivery of the synergistic drug pair doxorubicin (DOX) and gemcitabine (GEM). A 2D assay was adopted to evaluate therapeutic efficacy by determining combination index (CI), and a 3D assay using cancer spheroids was implemented to assess the potential for translation in vivo. The release of DOX and GEM from an acetylated-chitosan (ACS, degree of acetylation χAc = 40 ± 5%) was characterized to identify a combined drug loading that affords release kinetics and dose that are therapeutically synergistic. The selected DOX/GEM-ACS formulation was evaluated in vitro with 2-D and 3-D models of TNBC to determine the combination index (CI) and the tumor volume reduction, respectively. Therapeutically desired release dosages and kinetics of GEM and DOX were achieved. When evaluated with a 2-D model of TNBC, the hydrogel afforded a CI of 0.14, indicating a stronger synergism than concurrent administration of DOX and GEM (CI = 0.23). Finally, the therapeutic hydrogel accomplished a notable volume reduction of the cancer spheroids (up to 30%), whereas the corresponding dosages of free drugs only reduced growth rate. The ACS hydrogel delivery system accomplishes drug release kinetics and molar ratio that affords strong therapeutically synergism. These results, in combination with the choice of ACS as affordable and highly abundant source material, provide a strong pre-clinical demonstration of the potential of the proposed system for complementing surgical resection of aggressive solid tumors.}, number={7}, journal={PHARMACEUTICAL RESEARCH}, author={Schneible, John D. and Young, Ashlyn T. and Daniele, M. A. and Menegatti, S.}, year={2020} }
 @article{turner_kilgour_stine_daniele_menegatti_2020, title={Dual-Affinity Ratiometric Quenching (DARQ) Assay for the Quantification of Therapeutic Antibodies in CHO-S Cell Culture Fluids}, volume={92}, ISSN={["1520-6882"]}, DOI={10.1021/acs.analchem.0c04269}, abstractNote={More than 100 monoclonal antibodies (mAbs) are in industrial and clinical development to treat myriad diseases. Accurate quantification of mAbs in complex media, derived from industrial and patient samples, is vital to determine production efficiency or pharmacokinetic properties. To date, mAb quantification requires time and labor-intensive assays. Herein, we report a novel dual-affinity ratiometric quenching (DARQ) assay, which combines selective biorecognition and quenching of fluorescence signals for rapid and sensitive quantification of therapeutic monoclonal antibodies (mAbs). The reported assay relies on the affinity complexation of the target mAb by the corresponding antigens and Protein L (PrL, which targets the Fab region of the antibody), respectively, labeled with fluorescein and rhodamine. Within the affinity complex, the mAb acts as a scaffold framing the labeled affinity tags (PrL and antigen) in a molecular proximity that results in ratiometric quenching of their fluorescence emission. Notably, the decrease in fluorescence emission intensity is linearly dependent upon mAb concentration in solution. Control experiments conducted with one affinity tag only, two tags labeled with equal fluorophores, or two tags labeled with fluorophores of discrete absorbance and emission bands exhibited significantly reduced effect. The assay was evaluated in noncompetitive (pure mAb) and competitive conditions (mAb in a Chinese Hamster Ovary (CHO) cell culture harvest). The "DARQ" assay is highly reproducible (coefficient of variation ∼0.8-0.7%) and rapid (5 min), and its sensitivity (∼0.2-0.5 ng·mL-1), limit of detection (75-119 ng·mL-1), and dynamic range (300-1600 ng·mL-1) are independent of the presence of CHO host cell proteins.}, number={24}, journal={ANALYTICAL CHEMISTRY}, author={Turner, Brendan L. and Kilgour, Katie M. and Stine, Sydney J. and Daniele, Michael and Menegatti, Stefano}, year={2020}, month={Dec}, pages={16274–16283} }
 @misc{hunter_akbar_bhansali_daniele_erb_johnson_liu_miller_oralkan_hesketh_et al._2020, title={Editors' Choice-Critical Review-A Critical Review of Solid State Gas Sensors}, volume={167}, ISSN={["1945-7111"]}, DOI={10.1149/1945-7111/ab729c}, abstractNote={Solid state gas sensors are a core enabling technology to a range of measurement applications including industrial, safety, and environmental monitoring. The technology associated with solid-state gas sensors has evolved in recent years with advances in materials, and improvements in processing and miniaturization. In this review, we examine the state-of-the-art of solid state gas sensors with the goal of understanding the core technology and approaches, various sensor design methods to provide targeted functionality, and future prospects in the field. The structure, detection mechanism, and sensing properties of several types of solid state gas sensors will be discussed. In particular, electrochemical cells (solid and liquid), impedance/resistance based sensors (metal oxide, polymer, and carbon based structures), and mechanical sensing structures (resonators, cantilevers, and acoustic wave devices) as well as sensor arrays and supporting technologies, are described. Development areas for this field includes increased control of material properties for improved sensor response and durability, increased integration and miniaturization, and new material systems, including nano-materials and nano-structures, to address shortcomings of existing solid state gas sensors.}, number={3}, journal={JOURNAL OF THE ELECTROCHEMICAL SOCIETY}, author={Hunter, Gary W. and Akbar, Sheikh and Bhansali, Shekhar and Daniele, Michael and Erb, Patrick D. and Johnson, Kevin and Liu, Chung-Chiun and Miller, Derek and Oralkan, Omer and Hesketh, Peter J. and et al.}, year={2020}, month={Feb} }
 @misc{deal_brown_daniele_2020, title={Microphysiological systems for the modeling of wound healing and evaluation of pro-healing therapies}, volume={8}, ISSN={["2050-7518"]}, DOI={10.1039/d0tb00544d}, abstractNote={Wound healing microphysiological systems have been engineered with synthetic and natural materials and techniques such as bioprinting or viscous finger patterning. Model designs focus on particular phases of wound healing or the recapitulation of micro-anatomies.}, number={32}, journal={JOURNAL OF MATERIALS CHEMISTRY B}, author={Deal, Halston E. and Brown, Ashley C. and Daniele, Michael A.}, year={2020}, month={Aug}, pages={7062–7075} }
 @article{schneible_shi_young_ramesh_he_dowdey_dubnansky_libya_gao_santiso_et al._2020, title={Modified gaphene oxide (GO) particles in peptide hydrogels: a hybrid system enabling scheduled delivery of synergistic combinations of chemotherapeutics}, volume={8}, ISSN={["2050-7518"]}, DOI={10.1039/d0tb00064g}, abstractNote={Composite material enabling the delivery of synergistic combination of doxorubicin and gemcitabine against breast cancer with molar and kinetic precision.}, number={17}, journal={JOURNAL OF MATERIALS CHEMISTRY B}, author={Schneible, John D. and Shi, Kaihang and Young, Ashlyn T. and Ramesh, Srivatsan and He, Nanfei and Dowdey, Clay E. and Dubnansky, Jean Marie and Libya, Radina L. and Gao, Wei and Santiso, Erik and et al.}, year={2020}, month={May}, pages={3852–3868} }
 @misc{bacon_lavoie_rao_daniele_menegatti_2020, title={Past, Present, and Future of Affinity-based Cell Separation Technologies}, volume={112}, ISSN={["1878-7568"]}, DOI={10.1016/j.actbio.2020.05.004}, abstractNote={Progress in cell purification technology is critical to increase the availability of viable cells for therapeutic, diagnostic, and research applications. A variety of techniques are now available for cell separation, ranging from non-affinity methods such as density gradient centrifugation, dielectrophoresis, and filtration, to affinity methods such as chromatography, two-phase partitioning, and magnetic-/fluorescence-assisted cell sorting. For clinical and analytical procedures that require highly purified cells, the choice of cell purification method is crucial, since every method offers a different balance between yield, purity, and bioactivity of the cell product. For most applications, the requisite purity is only achievable through affinity methods, owing to the high target specificity that they grant. In this review, we discuss past and current methods for developing cell-targeting affinity ligands and their application in cell purification, along with the benefits and challenges associated with different purification formats. We further present new technologies, like stimuli-responsive ligands and parallelized microfluidic devices, towards improving the viability and throughput of cell products for tissue engineering and regenerative medicine. Our comparative analysis provides guidance in the multifarious landscape of cell separation techniques and highlights new technologies that are poised to play a key role in the future of cell purification in clinical settings and the biotech industry. STATEMENT OF SIGNIFICANCE: Technologies for cell purification have served science, medicine, and industrial biotechnology and biomanufacturing for decades. This review presents a comprehensive survey of this field by highlighting the scope and relevance of all known methods for cell isolation, old and new alike. The first section covers the main classes of target cells and compares traditional non-affinity and affinity-based purification techniques, focusing on established ligands and chromatographic formats. The second section presents an excursus of affinity-based pseudo-chromatographic and non-chromatographic technologies, especially focusing on magnetic-activated cell sorting (MACS) and fluorescence-activated cell sorting (FACS). Finally, the third section presents an overview of new technologies and emerging trends, highlighting how the progress in chemical, material, and microfluidic sciences has opened new exciting avenues towards high-throughput and high-purity cell isolation processes. This review is designed to guide scientists and engineers in their choice of suitable cell purification techniques for research or bioprocessing needs.}, journal={ACTA BIOMATERIALIA}, author={Bacon, Kaitlyn and Lavoie, Ashton and Rao, Balaji M. and Daniele, Michael and Menegatti, Stefano}, year={2020}, month={Aug}, pages={29–51} }
 @article{yuen_pozdin_young_turner_giles_naciri_trammell_charles_stenger_daniele_2020, title={Perylene-diimide-based n-type semiconductors with enhanced air and temperature stable photoconductor and transistor properties}, volume={174}, ISSN={["1873-3743"]}, DOI={10.1016/j.dyepig.2019.108014}, abstractNote={We report the synthesis and characterization of highly air and temperature stable, solution-processed, n-type organic semiconductors: a perylene-diimide monomer and a perylene-diimide-based pendant polymer. When integrated into a transistor structure, both materials possess pure n-type transport with mobility as high as 10−5 cm2 V−1 s−1 for the polymer. The organic semiconductors exhibit good photoconductor properties, with photocurrent to dark current ratios of up to 103 for the monomer, despite its lower FET mobility. The differences in transistor and photoconductor properties suggest different applications for each material. Both materials can be processed in air, and their transport properties have good air stability, improving with annealing even up to 200 °C in air. It is notable that such air-stable photoconductivity and transport properties have rarely been reported for n-type organic semiconductors before, as most n-type organic semiconductors are not stable in air. Hence, these materials may have potential in a wide range of applications.}, journal={DYES AND PIGMENTS}, author={Yuen, Jonathan D. and Pozdin, Vladimir A. and Young, Ashlyn T. and Turner, Brendan L. and Giles, Ian D. and Naciri, Jawad and Trammell, Scott A. and Charles, Paul T. and Stenger, David A. and Daniele, Michael A.}, year={2020}, month={Mar} }
 @article{day_schneible_young_pozdin_driessche_gaffney_prodromou_freytes_fourches_daniele_et al._2020, title={Photoinduced reconfiguration to control the protein-binding affinity of azobenzene-cyclized peptides}, volume={8}, ISSN={["2050-7518"]}, DOI={10.1039/d0tb01189d}, abstractNote={Light-controlled switching of cell-binding activity of fluorescently-labeled peptides for on-demand cell labeling.}, number={33}, journal={JOURNAL OF MATERIALS CHEMISTRY B}, author={Day, Kevin and Schneible, John D. and Young, Ashlyn T. and Pozdin, Vladimir A. and Driessche, George and Gaffney, Lewis A. and Prodromou, Raphael and Freytes, Donald O. and Fourches, Denis and Daniele, Michael and et al.}, year={2020}, month={Sep}, pages={7413–7427} }
 @article{young_white_daniele_2020, title={Rheological Properties of Coordinated Physical Gelation and Chemical Crosslinking in Gelatin Methacryloyl (GelMA) Hydrogels}, volume={20}, ISSN={["1616-5195"]}, DOI={10.1002/mabi.202000183}, abstractNote={Abstract}, number={12}, journal={MACROMOLECULAR BIOSCIENCE}, author={Young, Ashlyn T. and White, Olivia C. and Daniele, Michael A.}, year={2020}, month={Dec} }
 @article{yokus_songkakul_pozdin_bozkurt_daniele_2020, title={Wearable multiplexed biosensor system toward continuous monitoring of metabolites}, volume={153}, ISSN={["1873-4235"]}, DOI={10.1016/j.bios.2020.112038}, abstractNote={Comprehensive metabolic panels are the most reliable and common methods for monitoring general physiology in clinical healthcare. Translation of this clinical practice to personal health and wellness tracking requires reliable, non-invasive, miniaturized, ambulatory, and inexpensive systems for continuous measurement of biochemical analytes. We report the design and characterization of a wearable system with a flexible sensor array for non-invasive and continuous monitoring of human biochemistry. The system includes signal conditioning, processing, and transmission parts for continuous measurement of glucose, lactate, pH, and temperature. The system can operate three discrete electrochemical cells. The system draws 15 mA under continuous operation when powered by a 3.7 V 150 mAh battery. The analog front-end of the electrochemical cells has four potentiostats and three multiplexers for multiplexed and parallel readout from twelve working electrodes. Utilization of redundant working electrodes improves the measurement accuracy of sensors by averaging chronoamperometric responses across the array. The operation of the system is demonstrated in vitro by simultaneous measurement of glucose and lactate, pH, and skin temperature. In benchtop measurements, the sensors are shown to have sensitivities of 26.31 μA mM-1·cm-2 for glucose, 1.49 μA mM-1·cm-2 for lactate, 54 mV·pH-1 for pH, and 0.002 °C-1 for temperature. With the custom wearable system, these values were 0.84 ± 0.03 mV μM-1·cm-2 or glucose, 31.87 ± 9.03 mV mM-1·cm-2 for lactate, 57.18 ± 1.43 mV·pH-1 for pH, and 63.4 μV·°C-1 for temperature. This miniaturized wearable system enables future evaluation of temporal changes of the sweat biomarkers.}, journal={BIOSENSORS & BIOELECTRONICS}, author={Yokus, Murat A. and Songkakul, Tanner and Pozdin, Vladimir A. and Bozkurt, Alper and Daniele, Michael A.}, year={2020}, month={Apr} }
 @article{zwarycz_gracz_rivera_williamson_samsa_starmer_daniele_salter-cid_zhao_magness_2019, title={IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model}, volume={7}, ISSN={["2352-345X"]}, DOI={10.1016/j.jcmgh.2018.06.008}, abstractNote={Crohn's disease is an inflammatory bowel disease that affects the ileum and is associated with increased cytokines. Although interleukin (IL)6, IL17, IL21, and IL22 are increased in Crohn's disease and are associated with disrupted epithelial regeneration, little is known about their effects on the intestinal stem cells (ISCs) that mediate tissue repair. We hypothesized that ILs may target ISCs and reduce ISC-driven epithelial renewal.A screen of IL6, IL17, IL21, or IL22 was performed on ileal mouse organoids. Computational modeling was used to predict microenvironment cytokine concentrations. Organoid size, survival, proliferation, and differentiation were characterized by morphometrics, quantitative reverse-transcription polymerase chain reaction, and immunostaining on whole organoids or isolated ISCs. ISC function was assayed using serial passaging to single cells followed by organoid quantification. Single-cell RNA sequencing was used to assess Il22ra1 expression patterns in ISCs and transit-amplifying (TA) progenitors. An IL22-transgenic mouse was used to confirm the impact of increased IL22 on proliferative cells in vivo.High IL22 levels caused decreased ileal organoid survival, however, resistant organoids grew larger and showed increased proliferation over controls. Il22ra1 was expressed on only a subset of ISCs and TA progenitors. IL22-treated ISCs did not show appreciable differentiation defects, but ISC biomarker expression and self-renewal-associated pathway activity was reduced and accompanied by an inhibition of ISC expansion. In vivo, chronically increased IL22 levels, similar to predicted microenvironment levels, showed increases in proliferative cells in the TA zone with no increase in ISCs.Increased IL22 limits ISC expansion in favor of increased TA progenitor cell expansion.}, number={1}, journal={CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY}, author={Zwarycz, Bailey and Gracz, Adam D. and Rivera, Kristina R. and Williamson, Ian A. and Samsa, Leigh A. and Starmer, Josh and Daniele, Michael A. and Salter-Cid, Luisa and Zhao, Qihong and Magness, Scott T.}, year={2019}, pages={1–17} }
 @article{rivera_pozdin_young_erb_wisniewski_magness_daniele_2019, title={Integrated phosphorescence-based photonic biosensor (iPOB) for monitoring oxygen levels in 3D cell culture systems}, volume={123}, ISSN={["1873-4235"]}, DOI={10.1016/j.bios.2018.07.035}, abstractNote={Physiological processes, such as respiration, circulation, digestion, and many pathologies alter oxygen concentration in the blood and tissue. When designing culture systems to recapitulate the in vivo oxygen environment, it is important to integrate systems for monitoring and controlling oxygen concentration. Herein, we report the design and engineering of a system to remotely monitor and control oxygen concentration inside a device for 3D cell culture. We integrate a photonic oxygen biosensor into the 3D tissue scaffold and regulate oxygen concentration via the control of purging gas flow. The integrated phosphorescence-based oxygen biosensor employs the quenching of palladium-benzoporphyrin by molecular oxygen to transduce the local oxygen concentration in the 3D tissue scaffold. The system is validated by testing the effects of normoxic and hypoxic culture conditions on healthy and tumorigenic breast epithelial cells, MCF-10A cells and BT474 cells, respectively. Under hypoxic conditions, both cell types exhibited upregulation of downstream target genes for the hypoxia marker gene, hypoxia-inducible factor 1α (HIF1A). Lastly, by monitoring the real-time fluctuation of oxygen concentration, we illustrated the formation of hypoxic culture conditions due to limited diffusion of oxygen through 3D tissue scaffolds.}, journal={BIOSENSORS & BIOELECTRONICS}, author={Rivera, Kristina R. and Pozdin, Vladimir A. and Young, Ashlyn T. and Erb, Patrick D. and Wisniewski, Natalie A. and Magness, Scott T. and Daniele, Michael}, year={2019}, month={Jan}, pages={131–140} }
 @misc{rivera_yokus_erb_pozdin_daniele_2019, title={Measuring and regulating oxygen levels in microphysiological systems: design, material, and sensor considerations}, volume={144}, ISSN={["1364-5528"]}, DOI={10.1039/c8an02201a}, abstractNote={Quantifying and regulating oxygen in a microphysiological models can be achievedviaan array of technologies, and is an essential component of recapitulating tissue-specific microenvironments.}, number={10}, journal={ANALYST}, author={Rivera, Kristina R. and Yokus, Murat A. and Erb, Patrick D. and Pozdin, Vladimir A. and Daniele, Michael}, year={2019}, month={May}, pages={3190–3215} }
 @misc{young_rivera_erb_daniele_2019, title={Monitoring of Microphysiological Systems: Integrating Sensors and Real-Time Data Analysis toward Autonomous Decision-Making}, volume={4}, ISSN={["2379-3694"]}, DOI={10.1021/acssensors.8b01549}, abstractNote={Microphysiological systems replicate human organ function and are promising technologies for discovery of translatable biomarkers, pharmaceuticals, and regenerative therapies. Because microphysiological systems require complex microscale anatomical structures and heterogeneous cell populations, a major challenge remains to manufacture and operate these products with reproducible and standardized function. In this Perspective, three stages of microphysiological system monitoring, including process, development, and function, are assessed. The unique features and remaining technical challenges for the required sensors are discussed. Monitoring of microphysiological systems requires nondestructive, continuous biosensors and imaging techniques. With such tools, the extent of cellular and tissue development, as well as function, can be autonomously determined and optimized by correlating physical and chemical sensor outputs with markers of physiological performance. Ultimately, data fusion and analyses across process, development, and function monitors can be implemented to adopt microphysiological systems for broad research and commercial applications.}, number={6}, journal={ACS SENSORS}, author={Young, Ashlyn T. and Rivera, Kristina R. and Erb, Patrick D. and Daniele, Michael A.}, year={2019}, month={Jun}, pages={1454–1464} }
 @article{chester_marrow_daniele_brown_2019, title={Wound Healing and the Host Response in Regenerative Engineering}, DOI={10.1016/B978-0-12-801238-3.99896-9}, abstractNote={Wound healing is a complex and highly controlled process responsible for maintaining and reestablishing the homeostatic structure, function, and properties of tissues. This process becomes substantially more complicated upon the introduction of a foreign material. The purpose of this book chapter is to highlight the cellular processes and bioactive agents that encompass the wound healing process and to discuss how these processes change in the presence of a biomaterial. Common biomaterials that are used to direct the wound healing process are also discussed.}, journal={ENCYCLOPEDIA OF BIOMEDICAL ENGINEERING, VOL 1}, author={Chester, Daniel and Marrow, Ethan A. and Daniele, Michael A. and Brown, Ashley C.}, year={2019}, pages={707–718} }
 @article{su_huang_daniele_hensley_young_tang_allen_vandergriff_erb_ligler_et al._2018, title={Cardiac Stem Cell Patch Integrated with Microengineered Blood Vessels Promotes Cardiomyocyte Proliferation and Neovascularization after Acute Myocardial Infarction}, volume={10}, ISSN={["1944-8252"]}, DOI={10.1021/acsami.8b13571}, abstractNote={Cardiac stem cell (CSC) therapy has shown preclinical and clinical evidence for ischemic heart repair but is limited by low cellular engraftment and survival after transplantation. Previous versions of the cardiac patch strategy improve stem cell engraftment and encourage repair of cardiac tissue. However, cardiac patches that can enhance cardiomyogenesis and angiogenesis at the injured site remain elusive. Therapies that target cardiomyocyte proliferation and new blood vessel formation hold great potential for the protection against acute myocardial infarction (MI). Here, we report a new strategy for creating a vascularized cardiac patch in a facile and modular fashion by leveraging microfluidic hydrodynamic focusing to construct the biomimetic microvessels (BMVs) that include human umbilical vein endothelial cells (HUVECs) lining the luminal surface and then encapsulating the BMVs in a fibrin gel spiked with human CSCs. We show that the endothelialized BMVs mimicked the natural architecture and function of capillaries and that the resultant vascularized cardiac patch (BMV-CSC patch) exhibited equivalent release of paracrine factors compared to those of coculture of genuine human CSCs and HUVECs after 7 days of in vitro culture. In a rat model of acute MI, the BMV-CSC patch therapy induced profound mitotic activities of cardiomyocytes in the peri-infarct region 4 weeks post-treatment. A significant increase in myocardial capillary density was noted in the infarcted hearts that received BMV-CSC patch treatment compared to the infarcted hearts treated with conventional CSC patches. The striking therapeutic benefits and the fast and facile fabrication of the BMV-CSC patch make it promising for practical applications. Our findings suggest that the BMV-CSC patch strategy may open up new possibilities for the treatment of ischemic heart injury.}, number={39}, journal={ACS APPLIED MATERIALS & INTERFACES}, author={Su, Teng and Huang, Ke and Daniele, Michael A. and Hensley, Michael Taylor and Young, Ashlyn T. and Tang, Junnan and Allen, Tyler A. and Vandergriff, Adam C. and Erb, Patrick D. and Ligler, Frances S. and et al.}, year={2018}, month={Oct}, pages={33088–33096} }
 @article{young_cornwell_daniele_2018, title={Neuro Interfaces: Neuro-Nano Interfaces: Utilizing Nano-Coatings and Nanoparticles to Enable Next-Generation Electrophysiological Recording, Neural Stimulation, and Biochemical Modulation (Adv. Funct. Mater. 12/2018)}, volume={28}, ISSN={1616-301X}, url={http://dx.doi.org/10.1002/ADFM.201870079}, DOI={10.1002/ADFM.201870079}, abstractNote={In article number 1700239, Michael Daniele and co-workers evaluate the state-of-the-art in next-generation neuro-nano interfaces. Functional nanomaterials are being developed to improve neural implants, as well as, enable the modulation of neural activity and blood-brain barrier function via the remote transduction of optical and electromagnetic fields.}, number={12}, journal={Advanced Functional Materials}, publisher={Wiley}, author={Young, Ashlyn T. and Cornwell, Neil and Daniele, Michael A.}, year={2018}, month={Mar}, pages={1870079} }
 @article{young_cornwell_daniele_2018, title={Neuro-Nano Interfaces: Utilizing Nano-Coatings and Nanoparticles to Enable Next-Generation Electrophysiological Recording, Neural Stimulation, and Biochemical Modulation}, volume={28}, ISSN={1616-301X}, url={http://dx.doi.org/10.1002/ADFM.201700239}, DOI={10.1002/ADFM.201700239}, abstractNote={Abstract}, number={12}, journal={Advanced Functional Materials}, publisher={Wiley}, author={Young, Ashlyn T. and Cornwell, Neil and Daniele, Michael A.}, year={2018}, month={Mar}, pages={1700239} }
 @article{alves_turner_divito_daniele_walper_2017, title={Affinity purification of bacterial outer membrane vesicles (OMVs) utilizing a His-tag mutant}, volume={168}, DOI={10.1016/j.resmic.2016.10.001}, abstractNote={To facilitate the rapid purification of bacterial outer membrane vesicles (OMVs), we developed two plasmid constructs that utilize a truncated, transmembrane protein to present an exterior histidine repeat sequence. We chose OmpA, a highly abundant porin protein, as the protein scaffold and utilized the lac promoter to allow for inducible control of the epitope-presenting construct. OMVs containing mutant OmpA-His6 were purified directly from Escherichia coli culture media on an immobilized metal affinity chromatography (IMAC) Ni-NTA resin. This enabling technology can be combined with other molecular tools directed at OMV packaging to facilitate the separation of modified/cargo-loaded OMV from their wt counterparts. In addition to numerous applications in the pharmaceutical and environmental remediation industries, this technology can be utilized to enhance basic research capabilities in the area of elucidating endogenous OMV function.}, number={2}, journal={Research in Microbiology}, author={Alves, N. J. and Turner, K. B. and DiVito, K. A. and Daniele, M. A. and Walper, S. A.}, year={2017}, pages={139–146} }
 @article{yuen_walper_melde_daniele_stenger_2017, title={Electrolyte-Sensing Transistor Decals Enabled by Ultrathin Microbial Nanocellulose}, volume={7}, ISSN={2045-2322}, url={http://dx.doi.org/10.1038/SREP40867}, DOI={10.1038/SREP40867}, abstractNote={Abstract}, number={1}, journal={Scientific Reports}, publisher={Springer Science and Business Media LLC}, author={Yuen, Jonathan D. and Walper, Scott A. and Melde, Brian J. and Daniele, Michael A. and Stenger, David A.}, year={2017}, month={Jan} }
 @article{divito_daniele_roberts_ligler_adams_2017, title={Microfabricated blood vessels undergo neoangiogenesis}, volume={138}, ISSN={["1878-5905"]}, DOI={10.1016/j.biomaterials.2017.05.012}, abstractNote={The greatest ambition and promise of tissue engineering is to manufacture human organs. Before “made-to-measure” tissues can become a reality [1], [2], [3], however, three-dimensional tissues must be reconstructed and characterized. The current inability to manufacture operational vasculature has limited the growth of engineered tissues. Here, free-standing, small diameter blood vessels with organized cell layers that recapitulate normal biological functionality are fabricated using microfluidic technology. Over time in culture, the endothelial cells form a monolayer on the luminal wall and remodel the scaffold with human extracellular matrix proteins. After integration into three-dimensional gels containing fibroblasts, the microvessels sprout and generate extended hollow branches that anastomose with neighboring capillaries to form a network. Both the microfabricated vessels and the extended sprouts support perfusion of fluids and particles. The ability to create cellularized microvessels that can be designed with a diameter of choice, produced by the meter, and undergo angiogenesis and anastomoses will be an extremely valuable tool for vascularization of engineered tissues. To summarize, ultraviolet (UV) photo-crosslinkable poly(ethylene glycol) and gelatin methacrylate polymers used in combination with sheath-flow microfluidics allow for the fabrication of small diameter blood vessels which undergo neoangiogenesis as well as other developmental processes associated with normal human blood vessel maturation. Once mature, these vessels can be embedded; perfused; cryogenically stored and respond to stimuli such as chemokines and shear stresses to mimic native human blood vessels. The applications range from tissue-on-chip systems for drug screening, characterization of normal and pathologic processes, and creation and characterization of engineered tissues for organ repair.}, journal={BIOMATERIALS}, author={DiVito, Kyle A. and Daniele, Michael A. and Roberts, Steven A. and Ligler, Frances S. and Adams, Andre A.}, year={2017}, month={Sep}, pages={142–152} }
 @article{divito_daniele_roberts_ligler_adams_2017, title={“Data characterizing microfabricated human blood vessels created via hydrodynamic focusing”}, volume={14}, ISSN={2352-3409}, url={http://dx.doi.org/10.1016/J.DIB.2017.07.011}, DOI={10.1016/J.DIB.2017.07.011}, abstractNote={This data article provides further detailed information related to our research article titled "Microfabricated Blood Vessels Undergo Neovascularization" (DiVito et al., 2017) [1], in which we report fabrication of human blood vessels using hydrodynamic focusing (HDF). Hydrodynamic focusing with advection inducing chevrons were used in concert to encase one fluid stream within another, shaping the inner core fluid into 'bullseye-like" cross-sections that were preserved through click photochemistry producing streams of cellularized hollow 3-dimensional assemblies, such as human blood vessels (Daniele et al., 2015a, 2015b, 2014, 2016; Roberts et al., 2016) [2], [3], [4], [5], [6]. Applications for fabricated blood vessels span general tissue engineering to organ-on-chip technologies, with specific utility in in vitro drug delivery and pharmacodynamics studies. Here, we report data regarding the construction of blood vessels including cellular composition and cell positioning within the engineered vascular construct as well as functional aspects of the tissues.}, journal={Data in Brief}, publisher={Elsevier BV}, author={DiVito, Kyle A. and Daniele, Michael A. and Roberts, Steven A. and Ligler, Frances S. and Adams, André A.}, year={2017}, month={Oct}, pages={156–162} }
 @inproceedings{jandhyala_walper_cargill_ozual_daniele_2016, title={Integration of biochemical sensors into wearable biomaterial platforms}, volume={9863}, booktitle={Smart biomedical and physiological sensor technology xiii}, author={Jandhyala, S. and Walper, S. A. and Cargill, A. A. and Ozual, A. and Daniele, M. A.}, year={2016} }
 @article{roberts_divito_ligler_adams_daniele_2016, title={Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures}, volume={10}, ISSN={1932-1058}, url={http://dx.doi.org/10.1063/1.4963145}, DOI={10.1063/1.4963145}, abstractNote={Integrating a perfusable microvasculature system in vitro is a substantial challenge for “on-chip” tissue models. We have developed an inclusive on-chip platform that is capable of maintaining laminar flow through porous biosynthetic microvessels. The biomimetic microfluidic device is able to deliver and generate a steady perfusion of media containing small-molecule nutrients, drugs, and gases in three-dimensional cell cultures, while replicating flow-induced mechanical stimuli. Here, we characterize the diffusion of small molecules from the perfusate, across the microvessel wall, and into the matrix of a 3D cell culture.}, number={5}, journal={Biomicrofluidics}, publisher={AIP Publishing}, author={Roberts, Steven A. and DiVito, Kyle A. and Ligler, Frances S. and Adams, André A. and Daniele, Michael A.}, year={2016}, month={Sep}, pages={054109} }
 @inproceedings{keller_wilkins_reynolds_dieffenderfer_hood_daniele_bozkurt_tunc-ozdemir_2016, title={Nanocellulose electrodes for interfacing plant electrochemistry}, booktitle={2016 ieee sensors}, author={Keller, K. and Wilkins, M. and Reynolds, J. and Dieffenderfer, J. and Hood, C. and Daniele, M. A. and Bozkurt, A. and Tunc-Ozdemir, M.}, year={2016} }
 @misc{ngobili_daniele_2016, title={Nanoparticles and direct immunosuppression}, volume={241}, ISSN={["1535-3699"]}, DOI={10.1177/1535370216650053}, abstractNote={ Targeting the immune system with nanomaterials is an intensely active area of research. Specifically, the capability to induce immunosuppression is a promising complement for drug delivery and regenerative medicine therapies. Many novel strategies for immunosuppression rely on nanoparticles as delivery vehicles for small-molecule immunosuppressive compounds. As a consequence, efforts in understanding the mechanisms in which nanoparticles directly interact with the immune system have been overshadowed. The immunological activity of nanoparticles is dependent on the physiochemical properties of the nanoparticles and its subsequent cellular internalization. As the underlying factors for these reactions are elucidated, more nanoparticles may be engineered and evaluated for inducing immunosuppression and complementing immunosuppressive drugs. This review will briefly summarize the state-of-the-art and developments in understanding how nanoparticles induce immunosuppressive responses, compare the inherent properties of nanomaterials which induce these immunological reactions, and comment on the potential for using nanomaterials to modulate and control the immune system. }, number={10}, journal={EXPERIMENTAL BIOLOGY AND MEDICINE}, author={Ngobili, Terrika A. and Daniele, Michael A.}, year={2016}, month={May}, pages={1064–1073} }
 @article{chen_garland_geder_pruessner_mootz_cargill_leners_vokshi_davis_burns_et al._2016, title={Platinum Nanoparticle Decorated SiO2 Microfibers as Catalysts for Micro Unmanned Underwater Vehicle Propulsion}, volume={8}, ISSN={["1944-8252"]}, DOI={10.1021/acsami.6b10047}, abstractNote={Micro unmanned underwater vehicles (UUVs) need to house propulsion mechanisms that are small in size but sufficiently powerful to deliver on-demand acceleration for tight radius turns, burst-driven docking maneuvers, and low-speed course corrections. Recently, small-scale hydrogen peroxide (H2O2) propulsion mechanisms have shown great promise in delivering pulsatile thrust for such acceleration needs. However, the need for robust, high surface area nanocatalysts that can be manufactured on a large scale for integration into micro UUV reaction chambers is still needed. In this report, a thermal/electrical insulator, silicon oxide (SiO2) microfibers, is used as a support for platinum nanoparticle (PtNP) catalysts. The mercapto-silanization of the SiO2 microfibers enables strong covalent attachment with PtNPs, and the resultant PtNP-SiO2 fibers act as a robust, high surface area catalyst for H2O2 decomposition. The PtNP-SiO2 catalysts are fitted inside a micro UUV reaction chamber for vehicular propulsion; the catalysts can propel a micro UUV for 5.9 m at a velocity of 1.18 m/s with 50 mL of 50% (w/w) H2O2. The concomitance of facile fabrication, economic and scalable processing, and high performance-including a reduction in H2O2 decomposition activation energy of 40-50% over conventional material catalysts-paves the way for using these nanostructured microfibers in modern, small-scale underwater vehicle propulsion systems.}, number={45}, journal={ACS APPLIED MATERIALS & INTERFACES}, author={Chen, Bolin and Garland, Nathaniel T. and Geder, Jason and Pruessner, Marius and Mootz, Eric and Cargill, Allison and Leners, Anne and Vokshi, Granit and Davis, Jacob and Burns, Wyatt and et al.}, year={2016}, month={Nov}, pages={30941–30947} }
 @article{jenkins_bandera_daniele_ledford_tietje_kelso_sehorn_wei_chakrabarti_ray_et al._2016, title={Sequestering survivin to functionalized nanoparticles: a strategy to enhance apoptosis in cancer cells}, volume={4}, ISSN={2047-4830 2047-4849}, url={http://dx.doi.org/10.1039/C5BM00580A}, DOI={10.1039/C5BM00580A}, abstractNote={Survivin belongs to the family of inhibitor of apoptosis proteins (IAP) and is present in most cancers while being below detection limits in most terminally differentiated adult tissues, making it an attractive protein to target for diagnostic and, potentially, therapeutic roles.}, number={4}, journal={Biomaterials Science}, publisher={Royal Society of Chemistry (RSC)}, author={Jenkins, Ragini and Bandera, Yuriy P. and Daniele, Michael A. and Ledford, LeAnna L. and Tietje, Ashlee and Kelso, Andrew A. and Sehorn, Michael G. and Wei, Yanzhang and Chakrabarti, Mrinmay and Ray, Swapan K. and et al.}, year={2016}, pages={614–626} }
 @article{yokus_daniele_2016, title={Skin Hydration Sensor for Customizable Electronic Textiles}, volume={1}, ISSN={["2059-8521"]}, DOI={10.1557/adv.2016.540}, abstractNote={This paper introduces the design and simulated operation of a capacitive hydration sensor for integration into textile-based electronics. The multilayer patch is composed of a textile layer and an attached series of serpentine-interdigitated electrodes. The model used for simulations incorporated this design onto a representative model of skin. The serpentine-interdigitated electrodes are electrodes for capacitive measurement of skin hydration. In this study, the capacitance change relative to skin hydration was simulated using finite element analysis. The simulation results suggest the fabric layer had little effect on the capacitance of the sensor. Furthermore, the frequency domain simulations indicated that the capacitance of the sensor decreased with increasing frequency, and the decrease in capacitance was more significant for the dry skin compared to the wet skin. Therefore, the variation in the capacitance value of the serpentine-interdigitated electrodes can be employed for continuous skin hydration detection.}, number={38}, journal={MRS ADVANCES}, author={Yokus, Murat A. and Daniele, Michael A.}, year={2016}, pages={2671–2676} }
 @article{stover_fukuyama_young_daniele_oberley_crapo_baeumer_2016, title={Topically applied manganese-porphyrins BMX-001 and BMX-010 display a significant anti-inflammatory response in a mouse model of allergic dermatitis}, volume={308}, ISSN={["1432-069X"]}, DOI={10.1007/s00403-016-1693-0}, abstractNote={In this study, we topically administered two antioxidant compounds, the manganese-porphyrin-derivatives BMX-001 and BMX-010, in a mouse model of allergic dermatitis and compared the efficacy for reduction of itch and inflammation. In vitro effects of BMX-001 and BMX-010 on keratinocytes, bone marrow derived dendritic cells (BMDCs) and T-cells were initially analysed. For assessment of scratching behaviour, BMX-001 and BMX-010 (0.01 and 0.1 %) were topically applied 16 h and/or 1 h before compound 48/80 or toluene-2,4,-diisocyanate (TDI) challenge in a TDI induced mouse dermatitis model. Additionally, assessment of allergic skin inflammation was performed in a similar manner in the TDI model. Post-treatment ear thickness was measured 24 h after TDI challenge and compared to basal values. The mice were sacrificed and the ear auricle was removed for further analysis. In vitro, both BMX substances significantly inhibited cytokine production of keratinocytes as well as of BMDC and T-cell proliferation. Topical treatment with BMX cream resulted in a significant decrease in scratching behaviour in the compound 48/80 model, but not in the TDI model. Mice treated with BMX-001 and BMX-010 showed a moderate dose dependent decrease in ear thickness, and interestingly, the concentration of the cytokines IL-1β and IL-4 in inflamed skin was reduced by 80–90 % by all treatment options. These first results suggest the potential benefit of a BMX-001 and BMX-010 cream for the treatment of allergic-inflammatory skin diseases.}, number={10}, journal={ARCHIVES OF DERMATOLOGICAL RESEARCH}, author={Stover, Kelsey and Fukuyama, Tomoki and Young, Ashlyn T. and Daniele, Michael A. and Oberley, Rebecca and Crapo, James D. and Baeumer, Wolfgang}, year={2016}, month={Dec}, pages={711–721} }
 @inproceedings{dieffenderfer_wilkins_hood_beppler_daniele_bozkurt_2016, title={Towards a sweat-based wireless and wearable electrochemical sensor}, booktitle={2016 ieee sensors}, author={Dieffenderfer, J. and Wilkins, M. and Hood, C. and Beppler, E. and Daniele, M. A. and Bozkurt, A.}, year={2016} }
 @article{daniele_boyd_mott_ligler_2015, title={3D hydrodynamic focusing microfluidics for emerging sensing technologies}, volume={67}, ISSN={0956-5663}, url={http://dx.doi.org/10.1016/J.BIOS.2014.07.002}, DOI={10.1016/J.BIOS.2014.07.002}, abstractNote={While the physics behind laminar flows has been studied for 200 years, understanding of how to use parallel flows to augment the capabilities of microfluidic systems has been a subject of study primarily over the last decade. The use of one flow to focus another within a microfluidic channel has graduated from a two-dimensional to a three-dimensional process and the design principles are only now becoming established. This review explores the underlying principles for hydrodynamic focusing in three dimensions (3D) using miscible fluids and the application of these principles for creation of biosensors, separation of cells and particles for sample manipulation, and fabrication of materials that could be used for biosensors. Where sufficient information is available, the practicality of devices implementing fluid flows directed in 3D is evaluated and the advantages and limitations of 3D hydrodynamic focusing for the particular application are highlighted.}, journal={Biosensors and Bioelectronics}, publisher={Elsevier BV}, author={Daniele, Michael A. and Boyd, Darryl A. and Mott, David R. and Ligler, Frances S.}, year={2015}, month={May}, pages={25–34} }
 @article{alves_turner_daniele_oh_medintz_walper_2015, title={Bacterial Nanobioreactors–Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles}, volume={7}, ISSN={1944-8244 1944-8252}, url={http://dx.doi.org/10.1021/ACSAMI.5B08811}, DOI={10.1021/ACSAMI.5B08811}, abstractNote={All bacteria shed outer membrane vesicles (OMVs) loaded with a diverse array of small molecules, proteins, and genetic cargo. In this study we sought to hijack the bacterial cell export pathway to simultaneously produce, package, and release an active enzyme, phosphotriesterase (PTE). To accomplish this goal the SpyCatcher/SpyTag (SC/ST) bioconjugation system was utilized to produce a PTE-SpyCatcher (PTE-SC) fusion protein and a SpyTagged transmembrane porin protein (OmpA-ST), known to be abundant in OMVs. Under a range of physiological conditions the SpyTag and SpyCatcher domains interact with one another and form a covalent isopeptide bond driving packaging of PTE into forming OMVs. The PTE-SC loaded OMVs are characterized for size distribution, number of vesicles produced, cell viability, packaged PTE enzyme kinetics, OMV loading efficiency, and enzyme stability following iterative cycles of freezing and thawing. The PTE-loaded OMVs exhibit native-like enzyme kinetics when assayed with paraoxon as a substrate. PTE is often toxic to expression cultures and has a tendency to lose activity with improper handling. The coexpression of OmpA-ST with PTE-SC, however, greatly improved the overall PTE production levels by mitigating toxicity through exporting of the PTE-SC and greatly enhanced packaged enzyme stability against iterative cycles of freezing and thawing.}, number={44}, journal={ACS Applied Materials & Interfaces}, publisher={American Chemical Society (ACS)}, author={Alves, Nathan J. and Turner, Kendrick B. and Daniele, Michael A. and Oh, Eunkeu and Medintz, Igor L. and Walper, Scott A.}, year={2015}, month={Oct}, pages={24963–24972} }
 @article{daniele_boyd_adams_ligler_2015, title={Microfluidics: Microfluidic Strategies for Design and Assembly of Microfibers and Nanofibers with Tissue Engineering and Regenerative Medicine Applications (Adv. Healthcare Mater. 1/2015)}, volume={4}, ISSN={2192-2640}, url={http://dx.doi.org/10.1002/ADHM.201570002}, DOI={10.1002/ADHM.201570002}, abstractNote={Recent applications in tissue engineering and regenerative medicine have highlighted the utility of microfluidic fiber fabrication. On page 11 M. A. Daniele and team show how this process uses microflow shaping to generate a core-sheath profile, which can be subsequently solidified into microfibers of various shapes and chemistries, providing the capability to incorporate and organize both fragile biomolecules and cell-cultures within individual fibers and bioactive textiles.}, number={1}, journal={Advanced Healthcare Materials}, publisher={Wiley}, author={Daniele, Michael A. and Boyd, Darryl A. and Adams, André A. and Ligler, Frances S.}, year={2015}, month={Jan}, pages={2–2} }
 @article{daniele_knight_roberts_radom_erickson_2015, title={Nanocomposites: Sweet Substrate: A Polysaccharide Nanocomposite for Conformal Electronic Decals (Adv. Mater. 9/2015)}, volume={27}, ISSN={0935-9648}, url={http://dx.doi.org/10.1002/ADMA.201570064}, DOI={10.1002/ADMA.201570064}, abstractNote={On page 1600, M. A. Daniele and co-workers report the development of a flexible, robust, and green decal system for transferring circuitry to complex surfaces, such as biological tissue. The decal, a bilayer film of nanocellulose and pullulan, is characterized and utilized to support and transfer simple conductive traces. By taking full advantage of the chemical and mechanical robustness of the nanocellulose, these “polysaccharide circuit boards” show promise as a biocompatible substrate for wearable bioelectronics.}, number={9}, journal={Advanced Materials}, publisher={Wiley}, author={Daniele, Michael A. and Knight, Adrian J. and Roberts, Steven A. and Radom, Kathryn and Erickson, Jeffrey S.}, year={2015}, month={Mar}, pages={1636–1636} }
 @article{daniele_adams_naciri_north_ligler_2014, title={Interpenetrating networks based on gelatin methacrylamide and PEG formed using concurrent thiol click chemistries for hydrogel tissue engineering scaffolds}, volume={35}, ISSN={0142-9612}, url={http://dx.doi.org/10.1016/J.BIOMATERIALS.2013.11.009}, DOI={10.1016/J.BIOMATERIALS.2013.11.009}, abstractNote={The integration of biological extracellular matrix (ECM) components and synthetic materials is a promising pathway to fabricate the next generation of hydrogel-based tissue scaffolds that more accurately emulate the microscale heterogeneity of natural ECM. We report the development of a bio/synthetic interpenetrating network (BioSINx), containing gelatin methacrylamide (GelMA) polymerized within a poly(ethylene glycol) (PEG) framework to form a mechanically robust network capable of supporting both internal cell encapsulation and surface cell adherence. The covalently crosslinked PEG network was formed by thiol-yne coupling, while the bioactive GelMA was integrated using a concurrent thiol-ene coupling reaction. The physical properties (i.e. swelling, modulus) of BioSINx were compared to both PEG networks with physically-incorporated gelatin (BioSINP) and homogenous hydrogels. BioSINx displayed superior physical properties and significantly lower gelatin dissolution. These benefits led to enhanced cytocompatibility for both cell adhesion and encapsulation; furthermore, the increased physical strength provided for the generation of a micro-engineered tissue scaffold. Endothelial cells showed extensive cytoplasmic spreading and the formation of cellular adhesion sites when cultured onto BioSINx; moreover, both encapsulated and adherent cells showed sustained viability and proliferation.}, number={6}, journal={Biomaterials}, publisher={Elsevier BV}, author={Daniele, Michael A. and Adams, André A. and Naciri, Jawad and North, Stella H. and Ligler, Frances S.}, year={2014}, month={Feb}, pages={1845–1856} }
 @article{daniele_boyd_adams_ligler_2014, title={Microfluidic Strategies for Design and Assembly of Microfibers and Nanofibers with Tissue Engineering and Regenerative Medicine Applications}, volume={4}, ISSN={2192-2640}, url={http://dx.doi.org/10.1002/ADHM.201400144}, DOI={10.1002/ADHM.201400144}, abstractNote={Fiber‐based materials provide critical capabilities for biomedical applications. Microfluidic fiber fabrication has recently emerged as a very promising route to the synthesis of polymeric fibers at the micro and nanoscale, providing fine control over fiber shape, size, chemical anisotropy, and biological activity. This Progress Report summarizes advanced microfluidic methods for the fabrication of both microscale and nanoscale fibers and illustrates how different methods are enabling new biomedical applications. Microfluidic fabrication methods and resultant materials are explained from the perspective of their microfluidic device principles, including co‐flow, cross‐flow, and flow‐shaping designs. It is then detailed how the microchannel design and flow parameters influence the variety of synthesis chemistries that can be utilized. Finally, the integration of biomaterials and microfluidic strategies is discussed to manufacture unique fiber‐based systems, including cell scaffolds, cell encapsulation, and woven tissue matrices.}, number={1}, journal={Advanced Healthcare Materials}, publisher={Wiley}, author={Daniele, Michael A. and Boyd, Darryl A. and Adams, André A. and Ligler, Frances S.}, year={2014}, month={May}, pages={11–28} }
 @article{daniele_radom_ligler_adams_2014, title={Microfluidic fabrication of multiaxial microvessels via hydrodynamic shaping}, volume={4}, ISSN={2046-2069}, url={http://dx.doi.org/10.1039/C4RA03667K}, DOI={10.1039/C4RA03667K}, abstractNote={Fabrication of small, hydrogel microvessels (radii <250 um) through hydrodynamic shaping and photoinitiated polymerization is demonstrated. Photopolymerized hydrogel microvessels were produced and examined. The process is modular and amenable to generating an array of microvessel sizes and shapes.}, number={45}, journal={RSC Adv.}, publisher={Royal Society of Chemistry (RSC)}, author={Daniele, Michael A. and Radom, Kathryn and Ligler, Frances S. and Adams, André A.}, year={2014}, pages={23440–23446} }
 @article{claussen_daniele_geder_pruessner_mäkinen_melde_twigg_verbarg_medintz_2014, title={Platinum-Paper Micromotors: An Urchin-like Nanohybrid Catalyst for Green Monopropellant Bubble-Thrusters}, volume={6}, ISSN={1944-8244 1944-8252}, url={http://dx.doi.org/10.1021/AM504525E}, DOI={10.1021/AM504525E}, abstractNote={Platinum nanourchins supported on microfibrilated cellulose films (MFC) were fabricated and evaluated as hydrogen peroxide catalysts for small-scale, autonomous underwater vehicle (AUV) propulsion systems. The catalytic substrate was synthesized through the reduction of chloroplatinic acid to create a thick film of Pt coral-like microstructures coated with Pt urchin-like nanowires that are arrayed in three dimensions on a two-dimensional MFC film. This organic/inorganic nanohybrid displays high catalytic ability (reduced activation energy of 50-63% over conventional materials and 13-19% for similar Pt nanoparticle-based structures) during hydrogen peroxide (H2O2) decomposition as well as sufficient propulsive thrust (>0.5 N) from reagent grade H2O2 (30% w/w) fuel within a small underwater reaction vessel. The results demonstrate that these layered nanohybrid sheets are robust and catalytically effective for green, H2O2-based micro-AUV propulsion where the storage and handling of highly explosive, toxic fuels are prohibitive due to size-requirements, cost limitations, and close person-to-machine contact.}, number={20}, journal={ACS Applied Materials & Interfaces}, publisher={American Chemical Society (ACS)}, author={Claussen, Jonathan C. and Daniele, Michael A. and Geder, Jason and Pruessner, Marius and Mäkinen, Antti J. and Melde, Brian J. and Twigg, Mark and Verbarg, Jasenka M. and Medintz, Igor L.}, year={2014}, month={Sep}, pages={17837–17847} }
 @article{daniele_knight_roberts_radom_erickson_2014, title={Sweet Substrate: A Polysaccharide Nanocomposite for Conformal Electronic Decals}, volume={27}, ISSN={0935-9648}, url={http://dx.doi.org/10.1002/ADMA.201404445}, DOI={10.1002/ADMA.201404445}, abstractNote={A conformal electronic decal based on a polysaccharide circuit board (PCB) is fabricated and characterized. The PCBs are laminates composed of bioderived sugars - nanocellulose and pullulan. The PCB and decal transfer are a bioactive material system for supporting electronic devices capable of conforming to bio-logical surfaces.}, number={9}, journal={Advanced Materials}, publisher={Wiley}, author={Daniele, Michael A. and Knight, Adrian J. and Roberts, Steven A. and Radom, Kathryn and Erickson, Jeffrey S.}, year={2014}, month={Dec}, pages={1600–1606} }
 @article{daniele_shaughnessy_roeder_childress_bandera_foulger_2013, title={Magnetic Nanoclusters Exhibiting Protein-Activated Near-Infrared Fluorescence}, volume={7}, ISSN={1936-0851 1936-086X}, url={http://dx.doi.org/10.1021/nn3037368}, DOI={10.1021/nn3037368}, abstractNote={Composite nanoclusters with chemical, magnetic, and biofunctionality offer broad opportunities for targeted cellular imaging. A key challenge is to load a high degree of targeting, imaging, and therapeutic functionality onto stable metal-oxide nanoparticles. Here we report a route for producing magnetic nanoclusters (MNCs) with alkyne surface functionality that can be utilized as multimodal imaging probes. We form MNCs composed of magnetic Fe(3)O(4) nanoparticles and poly(acrylic acid-co-propargyl acrylate) by the co-precipitation of iron salts in the presence of copolymer stabilizers. The MNCs were surface-modified with near-infrared (NIR) emitting fluorophore used in photodynamic therapy, an azide-modified indocyanine green. The fluorophores engaged and complexed with bovine serum albumin, forming an extended coverage of serum proteins on the MNCs. These proteins isolated indocyanine green fluorophores from the aqueous environment and induced an effective "turn-on" of NIR emission.}, number={1}, journal={ACS Nano}, publisher={American Chemical Society (ACS)}, author={Daniele, Michael A. and Shaughnessy, Margaret L. and Roeder, Ryan and Childress, Anthony and Bandera, Yuriy P. and Foulger, Stephen}, year={2013}, month={Jan}, pages={203–213} }
 @article{daniele_north_naciri_howell_foulger_ligler_adams_2013, title={Microfabrication: Rapid and Continuous Hydrodynamically Controlled Fabrication of Biohybrid Microfibers (Adv. Funct. Mater. 6/2013)}, volume={23}, ISSN={1616-301X}, url={http://dx.doi.org/10.1002/ADFM.201370031}, DOI={10.1002/ADFM.201370031}, abstractNote={Hydrodynamic shaping is used as a versatile method for the in situ encapsulation of bacteria in microfibers. On page 698 André A. Adams and co-workers report the production of biohybrid microfibers by hydrodynamic shaping of a cell-containing pre-gel and inert sheath fluid, subsequently polymerized via photoinitiation.}, number={6}, journal={Advanced Functional Materials}, publisher={Wiley}, author={Daniele, Michael A. and North, Stella H. and Naciri, Jawad and Howell, Peter B. and Foulger, Stephen H. and Ligler, Frances S. and Adams, André A.}, year={2013}, month={Feb}, pages={697–697} }
 @article{jetty_bandera_daniele_hanor_hung_ramshesh_duperreault_nieminen_lemasters_foulger_2013, title={Protein triggered fluorescence switching of near-infrared emitting nanoparticles for contrast-enhanced imaging}, volume={1}, ISSN={2050-750X 2050-7518}, url={http://dx.doi.org/10.1039/C3TB20681E}, DOI={10.1039/C3TB20681E}, abstractNote={Sub-100 nm colloidal particles which are surface-functionalized with multiple environmentally-sensitive moieties have the potential to combine imaging, early detection, and the treatment of cancer with a single type of long-circulating "nanodevice". Deep tissue imaging is achievable through the development of particles which are surface-modified with fluorophores that operate in the near-infrared (NIR) spectrum and where the fluorophore's signal can be maximized by "turning-on" the fluorescence only in the targeted tissue. We present a general approach for the synthesis of NIR emitting nanoparticles that exhibit a protein triggered activation/deactivation of the emission. Dispersing the particles into an aqueous solution, such as phosphate buffered saline (PBS), resulted in an aggregation of the hydrophobic fluorophores and a cessation of emission. The emission can be reinstated, or activated, by the conversion of the surface-attached fluorophores from an aggregate to a monomeric species with the addition of an albumin. This activated probe can be deactivated and returned to a quenched state by a simple tryptic digestion of the albumin. The methodology for emission switching offers a path to maximize the signal from the typically weak quantum yield inherent in NIR fluorophores.}, number={36}, journal={Journal of Materials Chemistry B}, publisher={Royal Society of Chemistry (RSC)}, author={Jetty, Ragini and Bandera, Yuriy P. and Daniele, Michael A. and Hanor, David and Hung, Hsin-I. and Ramshesh, Venkat and Duperreault, Megan F. and Nieminen, Anna-Liisa and Lemasters, John J. and Foulger, Stephen H.}, year={2013}, pages={4542} }
 @article{daniele_bandera_sharma_rungta_roeder_sehorn_foulger_2012, title={Enzyme Recognition: Substrate-Baited Nanoparticles: A Catch and Release Strategy for Enzyme Recognition and Harvesting (Small 13/2012)}, volume={8}, ISSN={1613-6810}, url={http://dx.doi.org/10.1002/smll.201290072}, DOI={10.1002/smll.201290072}, abstractNote={The cover image illustrates a universal model for concentrating and extracting protein–substrate pairs via substrate-baited nanoparticles, which can be an invaluable tool in recognizing unknown protein–substrate affinities. The isolation of a single type of protein from a complex mixture is vital for the characterization of the function, structure, and interactions of the protein of interest and is typically the most laborious aspect of the protein purification process. Enzyme trapping and recycling is illustrated with the carbazole 1,9a-dioxygenase (CARDO) system, an enzyme important in bioremediation and natural product synthesis. The enzymes are baited by carbazolyl-moieties attached to nanoparticles through a click transformation. The single-step procedure for attracting the enzymes to the particles is capable of concentrating the protein from raw lysate and sequestering all required components of the protein to maintain bioactivity. For more information, please read the Full Paper “Substrate-Baited Nanoparticles: A Catch and Release Strategy for Enzyme Recognition and Harvesting” by S. H. Foulger, and co-workers, beginning on page 2083.}, number={13}, journal={Small}, publisher={Wiley}, author={Daniele, Michael A. and Bandera, Yuriy P. and Sharma, Deepti and Rungta, Parul and Roeder, Ryan and Sehorn, Michael G. and Foulger, Stephen H.}, year={2012}, month={Jul}, pages={1961–1961} }
 @article{daniele_north_naciri_howell_foulger_ligler_adams_2012, title={Rapid and Continuous Hydrodynamically Controlled Fabrication of Biohybrid Microfibers}, volume={23}, ISSN={1616-301X}, url={http://dx.doi.org/10.1002/adfm.201202258}, DOI={10.1002/adfm.201202258}, abstractNote={Abstract}, number={6}, journal={Advanced Functional Materials}, publisher={Wiley}, author={Daniele, Michael A. and North, Stella H. and Naciri, Jawad and Howell, Peter B. and Foulger, Stephen H. and Ligler, Frances S. and Adams, André A.}, year={2012}, month={Sep}, pages={698–704} }
 @article{daniele_bandera_sharma_rungta_roeder_sehorn_foulger_2012, title={Substrate-Baited Nanoparticles: A Catch and Release Strategy for Enzyme Recognition and Harvesting}, volume={8}, ISSN={1613-6810}, url={http://dx.doi.org/10.1002/smll.201200013}, DOI={10.1002/smll.201200013}, abstractNote={Abstract}, number={13}, journal={Small}, publisher={Wiley}, author={Daniele, Michael A. and Bandera, Yuriy P. and Sharma, Deepti and Rungta, Parul and Roeder, Ryan and Sehorn, Michael G. and Foulger, Stephen H.}, year={2012}, month={Apr}, pages={2083–2090} }