@article{chilton_petit_chilton_dessaux_2001, title={Structure and characterization of the crown gall opines heliopine, vitopine and rideopine}, volume={58}, ISSN={["0031-9422"]}, DOI={10.1016/S0031-9422(01)00166-2}, abstractNote={The crown gall opines heliopine from tumors induced by octopine type Agrobacterium tumefaciens strains A6, A136(pTiB6-806), E9, A652 and 1590-1 and vitopine from tumor induced by grapevine strains S4 and T2 are identical to synthetic N 2-(1′R-carboxyethyl)-l-glutamine. Tumors produced by strains S4 and T2 do not contain octopine or lysopine, but they do contain heliopine and the new opine ridéopine identified as N-(4′-aminobutyl)-d-glutamic acid. Grapevine strains S4 and T2 grow normally on tumor heliopine or synthetic heliopine and on tumor and synthetic ridéopine as well as on ridéopine lactam as sole carbon source. While octopine strains A6 and A136(pTiB6-806) do not grow on heliopine, mutant colonies do appear after a few weeks. Heliopine catabolism by octopine strains is not induced by octopine.}, number={1}, journal={PHYTOCHEMISTRY}, author={Chilton, WS and Petit, A and Chilton, MD and Dessaux, Y}, year={2001}, month={Sep}, pages={137–142} } @article{tilghman_astin_brinkley_chilton_cummings_ehrenberg_fox_glenn_green_hans_et al._1998, title={Trends in the early careers of life scientists - Preface and executive summary}, volume={9}, number={11}, journal={Molecular Biology of the Cell}, author={Tilghman, S. and Astin, H. S. and Brinkley, W. and Chilton, M. D. and Cummings, M. P. and Ehrenberg, R. G. and Fox, M. F. and Glenn, K. and Green, P. J. and Hans, S. and et al.}, year={1998}, pages={3007–3015} } @article{melanson_chilton_mastersmoore_chilton_1997, title={A deletion in an indole synthase gene is responsible for the DIMBOA-deficient phenotype of bxbx maize}, volume={94}, ISSN={["0027-8424"]}, DOI={10.1073/pnas.94.24.13345}, abstractNote={The biosynthesis of DIMBOA, a pesticidal secondary metabolite of maize, branches off the tryptophan pathway. We have previously demonstrated that indole is the last intermediate common to both the tryptophan and hydroxamic acid pathways. The earliest discovered mutant in the DIMBOA pathway, bxbx (benzoxazineless), is deficient in the production of DIMBOA and related compounds. This paper presents evidence that a gene identified by Kramer and Koziel [Kramer, V. C. & Koziel, M. G. (1995) Plant Mol. Biol. 27, 1183-1188] as maize tryptophan synthase alpha (TSA) is the site of the genetic lesion in the DIMBOA-deficient mutant maize line bxbx. We demonstrate that the TSA gene has sustained a 924-bp deletion in bxbx compared with its counterpart in wild-type maize. We report that the TSA gene maps to the same location as the bxbx mutation, on the short arm of chromosome 4. We present evidence that the very early and very high level of expression of TSA corresponds to the timing and level of DIMBOA biosynthesis but is strikingly different from the expression of the maize tryptophan synthase beta (TSB) genes. We show that feeding indole to bxbx seedlings restores their ability to synthesize DIMBOA. We conclude that the maize enzyme initially named tryptophan synthase alpha in fact is a DIMBOA biosynthetic enzyme, and we propose that it be renamed indole synthase. This work confirms and enlarges upon the findings of Frey et al. [Frey, M., Chomet, P., Glawischniq, E., Stettner, C., Grün, S., Winklmair, A., Eisenreich, W., Bacher, A., Meeley, R. B., Briggs, S. P., Simcox, K. & Gierl, A. (1997) Science 277, 696-699], which appeared while the present paper was in review.}, number={24}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Melanson, D and Chilton, MD and MastersMoore, D and Chilton, WS}, year={1997}, month={Nov}, pages={13345–13350} }