Works (59)

Updated: September 15th, 2024 06:25

2024 article

CRISPR/Cas9-mediated genome editing of <i>Frankliniella occidentalis</i>, the western flower thrips, via embryonic microinjection

Han, J., Klobasa, W., Oliveira, L., Rotenberg, D., Whitfield, A. E., & Lorenzen, M. D. (2024, April 27). INSECT MOLECULAR BIOLOGY, Vol. 4.

By: J. Han n, W. Klobasa n, L. Oliveira n, D. Rotenberg n, A. Whitfield n & M. Lorenzen n

author keywords: CRISPR; eye; gene knockout; insect vector; Thysanoptera
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 7, 2024

2024 journal article

Development of an agroinfiltration-based transient hairpin RNA expression system in pak choi leaves<i> (Brassica</i><i> rapa</i> ssp.<i> chinensis)</i> for RNA interference against Liriomyza sativae

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 204.

author keywords: Agroinfiltration-mediated RNAi; Liriomyza sativae; V-ATPase a; Actin; Pest control
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: September 9, 2024

2024 journal article

Evaluation of <i>Peregrinus maidis transformer-2</i> as a target for CRISPR-based control

PLOS ONE, 19(4).

By: Y. Wang n, D. Rivera n, W. Klobasa n & M. Lorenzen n

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 17, 2024

2024 journal article

The Genome of <i>Arsenophonus</i> sp. and Its Potential Contribution in the Corn Planthopper, <i>Peregrinus maidis</i>

INSECTS, 15(2).

By: Y. Wang n, A. Mikaelyan n, B. Coates* & M. Lorenzen n

author keywords: genome assembly; Arsenophonus sp.; Peregrinus maidis; hemiptera
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 6, 2024

2023 journal article

A draft Diabrotica virgifera virgifera genome: insights into control and host plant adaption by a major maize pest insect

BMC Genomics, 24(1).

author keywords: Genome assembly; Host plant specialization; Differential expression
MeSH headings : Animals; Zea mays / physiology; Coleoptera / genetics; Larva / metabolism; Poaceae / genetics; Insecticides / metabolism; Pest Control, Biological; Plants, Genetically Modified / genetics; Endotoxins
TL;DR: Insight is provided into transcriptomic responses of larval feeding on different host plants and resources for genomic research on this economically significant pest of maize and suggests that adaptations of D. virgifera larvae to feeding on an alternate host plant invoke fewer transcriptional changes compared to marginal or poor hosts. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 13, 2023

2023 journal article

An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera

Insects, 14(8), 683.

author keywords: insect rearing; rootworm; molecular biology; function genomics; CRISPR
TL;DR: An optimized rearing system for use in western corn rootworm functional genomics research is reported, specifically the development of a system that facilitates the collection of preblastoderm embryos for microinjection as well as gathering large larvae and pupae for downstream phenotypic screening. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 11, 2023

2023 journal article

Development of transgenic corn planthopper <scp><i>Peregrinus maidis

Insect Molecular Biology, 32(4), 363–375.

By: A. Patil n, W. Klobasa n, D. Espinoza‐Rivera n, O. Baars n, M. Lorenzen n & M. Scott n

TL;DR: The aim of this study was to develop transgenic P. maidis strains that could be used for future genetic biocontrol programs and is the first report of a transgenic Hemiptera. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: March 11, 2023

2023 journal article

Genome and Genetic Engineering of the House Cricket (Acheta domesticus): A Resource for Sustainable Agriculture

Biomolecules, 13(4), 589.

author keywords: food security; cricket; protein; genome; sustainability; agriculture; insects as food and feed; Acheta domesticus; insect genome; CRISPR-Cas9; genetic engineering
MeSH headings : Animals; Gryllidae / genetics; Gryllidae / metabolism; Agriculture; Crops, Agricultural; Allergens / metabolism; Genetic Engineering
TL;DR: The first high quality annotated genome assembly of A. domesticus is presented, demonstrating both CRISPR/Cas9-mediated knock-in and knock-out of the house cricket and implications for the food, pharmaceutical, and other industries. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries, Crossref
Added: March 29, 2023

2023 journal article

Structural and functional insights into the ATP-binding cassette transporter family in the corn planthopper, Peregrinus maidis

Insect Molecular Biology, 32(4), 412–423.

By: Y. Wang n, W. Klobasa n, F. Chu n, O. Huot n, A. Whitfield n & M. Lorenzen n

author keywords: ABC transporters; Hemiptera; RNA interference (RNAi); transcriptome
TL;DR: Transcriptomic data and RNAi results presented here will no doubt assist with the development of new control methods for this pest and each phenotype is similar to that found when targeting the orthologous gene in other species and it demonstrates their potential in RNAi‐based P. maidis control. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 1, 2023

2022 journal article

The Genome of Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae): Adaptation for Success

Genes, 13(3), 446.

author keywords: Bostrichidae; insect reference genome; insecticide resistance; insect genetics; lesser grain borer; Rhyzopertha dominica
MeSH headings : Acclimatization; Animals; Coleoptera / genetics; Dominica; Insecticides; Larva / genetics
TL;DR: The lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), is a major global pest of cereal grains, and the genome sequenced to identify genes responsible for important biological functions and develop more targeted and efficacious management strategies. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 18, 2022

2021 journal article

Microinjection of Corn Planthopper, <em>Peregrinus maidis</em>, Embryos for CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, 3(169).

MeSH headings : Animals; CRISPR-Cas Systems / genetics; Endonucleases / genetics; Female; Gene Editing / methods; Zea mays / chemistry
TL;DR: Detailed protocols for collecting and microinjecting precellular P. Maidis embryos with CRISPR components (Cas9 nuclease that has been complexed with guide RNAs), and results of Cas9-based gene knockout of a P. maidis eye-color gene, white, are presented. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 17, 2021

2020 journal article

Characterization, expression patterns, and transcriptional responses of three core RNA interference pathway genes from Ostrinia nubilalis

Journal of Insect Physiology, 129, 104181.

author keywords: RNAi efficiency; Dicer 2; Argonaute 2; R2D2; European corn borer; Western corn rootworm; Diabrotica virgifera virgifera
MeSH headings : Animals; Argonaute Proteins / genetics; Genes, Insect / drug effects; Insect Control / methods; Moths / genetics; Moths / metabolism; RNA Helicases / genetics; RNA Interference; RNA, Small Interfering / pharmacology; RNA-Binding Proteins / genetics; RNAi Therapeutics
TL;DR: To determine if deficiencies in these core RNAi pathway genes contribute to low RNAi efficiency in ECB, full-length complementary DNAs encoding OnAgo2, OnDcr2, and OnR2D2 were cloned, sequenced, and characterized and indicated that transcripts for all three genes were expressed in all developmental stages and tissues investigated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 10, 2021

2020 journal article

Core commitments for field trials of gene drive organisms

Science, 370(6523), 1417–1419.

By: K. Long, L. Alphey, G. Annas, C. Bloss, K. Campbell, J. Champer, C. Chen, A. Choudhary ...

Contributors: K. Long, L. Alphey, G. Annas, C. Bloss, K. Campbell, J. Champer, C. Chen, A. Choudhary ...

MeSH headings : Animals; Gene Drive Technology / standards; Guidelines as Topic; Organisms, Genetically Modified; Safety
TL;DR: Significant commitments are outlined that are deemed critical for responsible conduct of a field trial and to ensure that these technologies, if they are introduced, serve the public interest. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: March 22, 2021

2020 journal article

Molecular Characterizations of Double-Stranded RNA Degrading Nuclease Genes from Ostrinia nubilalis

Insects, 11(10), 652.

author keywords: dsRNase; European corn borer; gene expression; REase; RNAi efficiency; substrate specificity
TL;DR: This study characterized genes encoding proteins from European corn borer that are associated with RNA digestion in other insects and investigated the contribution of dsRNA-degrading endonucleases (dsRNases) and lepidopteran-specific RNAi efficiency-related nucleases (REases) to ds RNA instability and lowRNAi efficiency in ECB. (via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: September 28, 2020

2020 journal article

New Technologies for Studying Negative-Strand RNA Viruses in Plant and Arthropod Hosts

Molecular Plant-Microbe Interactions®, 33(3), 382–393.

By: T. German*, M. Lorenzen n, N. Grubbs n & A. Whitfield n

author keywords: virus-plant interactions
MeSH headings : Animals; Insect Vectors / virology; Insecta / virology; Plant Diseases / virology; Plant Viruses; Plants / virology; RNA Viruses
TL;DR: Functional analysis of genes using genome editing will provide future pathways for further study of the transmission cycle and new control strategies for these viruses and their vectors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 3, 2020

2020 journal article

Transcriptome analysis of life stages of the house cricket, Acheta domesticus, to improve insect crop production

Scientific Reports, 10(1).

By: B. Oppert*, L. Perkin*, M. Lorenzen n & A. Dossey*

MeSH headings : Animals; Antimicrobial Cationic Peptides / antagonists & inhibitors; Antimicrobial Cationic Peptides / genetics; Antimicrobial Cationic Peptides / metabolism; Crop Production; Embryo, Nonmammalian / metabolism; Female; Gene Expression Profiling; Gryllidae / genetics; Gryllidae / growth & development; Gryllidae / metabolism; Insect Proteins / antagonists & inhibitors; Insect Proteins / genetics; Insect Proteins / metabolism; Life Cycle Stages; Male; Nymph / genetics; Nymph / metabolism; RNA Interference; Transcriptome
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: September 14, 2020

2019 journal article

Effects of targeting eye color in Tenebrio molitor through RNA interference of tryptophan 2,3‐dioxygenase ( vermilion ): Implications for insect farming

Archives of Insect Biochemistry and Physiology, 101(1), e21546.

By: B. Oppert*, F. Chu n, S. Reyna n, S. Pinzi n, S. Adrianos*, L. Perkin*, M. Lorenzen n

author keywords: agriculture; entomophagy; farming; gene expression; insect eye color; protein; RNA-Seq; Tenebrio molitor; vermilion
MeSH headings : Animal Husbandry; Animals; Eye Color / genetics; Gene Expression; Genetic Markers; Insect Proteins / genetics; Insect Proteins / metabolism; Larva / genetics; Pigmentation / genetics; Pupa / genetics; RNA Interference; Tenebrio / enzymology; Tenebrio / genetics; Tenebrio / growth & development; Tryptophan Oxygenase / genetics
TL;DR: A dominant marker system based on eye color can be developed for the genetic manipulation of T. molitor to increase the value of mealworms as an alternative food source by decreasing negative traits,such as disease susceptibility, and increasing desired traits, such as protein content and vitamin production. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 15, 2019

2019 journal article

Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene family

BMC Genomics, 20(1).

By: F. Adedipe n, N. Grubbs n, B. Coates*, B. Wiegmman & M. Lorenzen n

author keywords: ATP-binding cassette (ABC) transporter; Phylogenetic; Transcriptome; RNA interference (RNAi); Corn rootworm
MeSH headings : ATP-Binding Cassette Transporters / genetics; Animals; Coleoptera / classification; Coleoptera / genetics; Coleoptera / growth & development; Computational Biology / methods; Gene Expression Profiling; Gene Ontology; Life Cycle Stages / genetics; Molecular Sequence Annotation; Multigene Family; Phenotype; RNA Interference; Transcriptome
TL;DR: This study has produced a relatively comprehensive de novo transcriptome assembly for D. v. virgifera and identified 65 members of the ABC transporter family and provided the first insights into the developmental and physiological roles of ABC transporters in this pest species. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: December 16, 2019

2019 journal article

The distribution and spread of naturally occurring <i>Medea</i> selfish genetic elements in the United States

Ecology and Evolution, 9(24), 14407–14416.

By: S. Cash n, M. Lorenzen n & F. Gould n

author keywords: maternal effect; Medea; red flour beetle; selfish genetic element
TL;DR: The finding of patchy distributions of Medea elements suggests that released synthetic SGEs cannot always be expected to spread uniformly, especially in target species with limited dispersal. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 10, 2020

2019 journal article

The impact of local population genetic background on the spread of the selfish element <i>Medea‐1</i> in red flour beetles

Ecology and Evolution, 10(2), 863–874.

By: S. Cash n, M. Robert*, M. Lorenzen n & F. Gould n

author keywords: gene drive; maternal effect; Medea; selfish genetic element
TL;DR: It is shown that populations with naturally low to intermediate M1 frequencies likely represent transient states during the process of Medea spread and how a known suppressor of MedEA can impair the increase of M1 in populations is shown. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 6, 2020

2019 content posted

Window to the Fluorescence: The<i>White

TL;DR: The discovery, cloning, and analysis of Dvvw cDNA and promoter are reported, and RNA interference is utilized to knock down DvvW mRNA in a transgenic strain, thereby demonstrating the utility of eye-color mutations when developing transgenic technologies. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 23, 2023

2018 journal article

Cover Image

Archives of Insect Biochemistry and Physiology, 97(3), e21453.

By: F. Chu, W. Klobasa, N. Grubbs & M. Lorenzen

Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2018 journal article

Genome of the small hive beetle (Aethina tumida, Coleoptera: Nitidulidae), a worldwide parasite of social bee colonies, provides insights into detoxification and herbivory

GigaScience, 7(12).

By: J. Evans*, D. McKenna*, E. Scully*, S. Cook*, B. Dainat, N. Egekwu*, N. Grubbs n, D. Lopez* ...

author keywords: Coleoptera; pollination; Apis mellifera; invasive pest; phytophagy; glycoside hydrolase; honey bee
MeSH headings : ATP-Binding Cassette Transporters / classification; ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Acetylcholinesterase / classification; Acetylcholinesterase / genetics; Acetylcholinesterase / metabolism; Animals; Bees / parasitology; Coleoptera / classification; Coleoptera / genetics; Genetic Variation; Genome; Glycoside Hydrolases / classification; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Herbivory; Insect Proteins / classification; Insect Proteins / genetics; Insect Proteins / metabolism; Insecticides / metabolism; Phylogeny; Receptors, Cell Surface / classification; Receptors, Cell Surface / genetics; Receptors, Cell Surface / metabolism; Voltage-Gated Sodium Channels / classification; Voltage-Gated Sodium Channels / genetics
TL;DR: The ATUMI genome is sequenced and annotated, providing the first genomic resources for this species and for the Nitidulidae, a beetle family that is closely related to the extraordinarily species-rich clade of beetles known as the Phytophaga. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: February 11, 2019

2018 journal article

Metabolic pathway interruption: CRISPR/Cas9-mediated knockout of tryptophan 2,3-dioxygenase in Tribolium castaneum

JOURNAL OF INSECT PHYSIOLOGY, 107, 104–109.

By: S. Adrianos*, M. Lorenzen n & B. Oppert*

author keywords: CRISPR; Ommochrome; Tryptophan 2,3-dioxygenase; Vermilion; Tribolium castaneum; Insect eye pigment
MeSH headings : Amino Acid Sequence; Animals; CRISPR-Cas Systems; Female; Gene Knockout Techniques; Insect Proteins / chemistry; Insect Proteins / genetics; Insect Proteins / metabolism; Larva / genetics; Larva / growth & development; Larva / physiology; Male; Metabolic Networks and Pathways; Ocular Physiological Phenomena; Phylogeny; Pigmentation / genetics; Sequence Alignment; Tribolium / genetics; Tribolium / growth & development; Tribolium / physiology; Tryptophan Oxygenase / chemistry; Tryptophan Oxygenase / genetics; Tryptophan Oxygenase / metabolism
TL;DR: The Tribolium castaneum vermilion gene encodes tryptophan 2,3-dioxygenase, a pivotal enzyme in the ommochrome pathway that is required for proper pigmentation of the eye and was used to establish a mutant strain homozygous for the CRISPR-induced knock-out allele. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

Microinjection of Western Corn Rootworm, <em>Diabrotica virgifera virgifera</em>, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, (134).

author keywords: Bioengineering; Issue 134; embryonic microinjection; functional genomics; germline transformation; transgenesis; CRISPR/Cas9 genome editing; western corn rootworm
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Coleoptera; Gene Editing / methods; Germ-Line Mutation / genetics; Microinjections / methods; RNA Interference
TL;DR: The methods used to collect and microinject precellular WCR embryos with nucleic acids are detailed, aimed at the creation of transgenic WCR, and CRISPR/Cas9-genome editing could also be performed using the same protocols, with the only difference being the composition of the solution injected into the embryos. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 16, 2018

2017 journal article

Agricultural production: assessment of the potential use of Cas9-mediated gene drive systems for agricultural pest control

Journal of Responsible Innovation, 5(sup1), S98–S120.

By: M. Scott n, F. Gould n, M. Lorenzen n, N. Grubbs n, O. Edwards* & D. O’Brochta*

author keywords: Gene drive; Cas9; sterile insect technique; agricultural pest insect
TL;DR: To highlight how gene drives could be useful for control of agricultural insect pests, species that are pests of animals, plants, and animals are selected. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2017 journal article

Development and use of a piggyBac -based jumpstarter system in Drosophila suzukii

Archives of Insect Biochemistry and Physiology, 97(3), e21439.

author keywords: germline transformation; Minos; mutagenesis; piggyBac; transgenesis
MeSH headings : Animals; Animals, Genetically Modified; DNA Transposable Elements; Drosophila / genetics; Genetic Engineering / methods; Mutagenesis; Transposases
TL;DR: It is demonstrated that there is a dramatic increase in transformation rates when germline transformation is performed in a transposase-expressing background, and it is shown that these jumpstarter strains could be used in conjunction with a piggyBac-based donor strain for genome-wide mutagenesis of D. suzukii. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera

Insect Molecular Biology, 26(4), 440–452.

By: F. Chu n, W. Klobasa n, P. Wu n, S. Pinzi n, N. Grubbs n, S. Gorski n, Y. Cardoza n, M. Lorenzen n

author keywords: Minos; piggyBac; small-scale rearing; embryonic microinjection
MeSH headings : Animals; Coleoptera / genetics; Female; Gene Transfer Techniques; Luminescent Proteins; Male; Transposases
TL;DR: Establishing transgenic technologies for this beetle is the first step towards bringing a wide range of transformation‐based tools to bear on understanding WCR biology. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2016 journal article

Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Insect Biochemistry and Molecular Biology, 76, 118–147.

By: M. Kanost*, E. Arrese*, X. Cao*, Y. Chen*, S. Chellapilla*, M. Goldsmith*, E. Grosse-Wilde*, D. Heckel* ...

author keywords: Lepidoptera; Insect; Tobacco hornworm; Synteny; Moth; Insect biochemistry; Innate immunity
MeSH headings : Animals; Gene Expression; Gene Expression Profiling; Genome, Insect; Larva / genetics; Larva / growth & development; Manduca / genetics; Manduca / growth & development; Pupa / genetics; Pupa / growth & development; Sequence Analysis, DNA; Synteny
TL;DR: The sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages, provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects. (via Semantic Scholar)
Source: Crossref
Added: January 25, 2020

2016 journal article

Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Insect Biochemistry and Molecular Biology, 76, 118–147.

By: M. Kanost, E. Arrese, X. Cao, Y. Chen, S. Chellapilla, M. Goldsmith, E. Grosse-Wilde, D. Heckel ...

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Genes related to mitochondrial functions are differentially expressed in phosphine-resistant and -susceptible Tribolium castaneum

BMC GENOMICS, 16.

By: B. Oppert*, R. Guedes*, M. Aikins*, L. Perkin*, Z. Chen*, T. Phillips*, K. Zhu*, G. Opit* ...

author keywords: Anti-diuretic peptide; Cytochrome P450; Deltamethrin; Dihydrolipoamide dehydrogenase; Fumigants; Insecticide resistance; Gene expression; Phosphine resistance; Red flour beetle; RNA-Seq; Stored product pests; Tribolium castaneum
MeSH headings : Amino Acid Sequence; Animals; Cytochrome P-450 Enzyme System / genetics; Dihydrolipoamide Dehydrogenase / chemistry; Dihydrolipoamide Dehydrogenase / genetics; Genomics; Insecticide Resistance / genetics; Mitochondria / drug effects; Mitochondria / metabolism; Molecular Sequence Data; Nitriles / pharmacology; Phosphines / pharmacology; Pyrethrins / pharmacology; Sequence Analysis, RNA; Transcriptome / drug effects; Tribolium / cytology; Tribolium / drug effects; Tribolium / enzymology; Tribolium / genetics
TL;DR: The first gene expression data on the response of phosphine-resistant and -susceptible insects to phosphine exposure is provided, and RNA-Seq is demonstrated to be a valuable tool to examine differences in insects that respond differentially to environmental stimuli. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

The ABCs of Eye Color in Tribolium castaneum: Orthologs of the Drosophila white, scarlet, and brown Genes

GENETICS, 199(3), 749-+.

By: N. Grubbs n, S. Haas*, R. Beeman* & M. Lorenzen n

author keywords: ABC transporters; eye pigmentation; evolution of development
MeSH headings : ATP-Binding Cassette Transporters / genetics; Animals; Base Sequence; Drosophila Proteins / genetics; Evolution, Molecular; Eye Color / genetics; Eye Proteins / genetics; Genes, Insect; Molecular Sequence Data; Mutation; Tribolium / genetics
TL;DR: RNA inhibition of Tcw and Tcst recapitulates the mutant phenotypes, confirming the roles of these genes in normal eye pigmentation, while RNA interference of Tcbw provides further evidence that it has no role in eye pigmented in Tribolium. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 book

Dictionary of Toxicology

(3rd ed.). Amsterdam: Academic Press.

By: E. Hodgson, M. Roe, R. Mailman & J. Chambers

Source: NC State University Libraries
Added: March 26, 2021

2014 conference paper

Genetics and Genomics of Tribolium Medea Elements

Presented at the 11th International Working Conference on Stored Product Protection, Chiang Mai, Thailand.

By: F. Chu, M. Lorenzen & W. Klobasa

Event: 11th International Working Conference on Stored Product Protection at Chiang Mai, Thailand on November 24-28, 2014

Source: NC State University Libraries
Added: March 26, 2021

2013 journal article

Functional analysis of the ATP-binding cassette (ABC) transporter gene family of Tribolium castaneum

BMC GENOMICS, 14.

By: G. Broehan*, T. Kroeger*, M. Lorenzen n & H. Merzendorfer*

author keywords: ABC transporter; Coleoptera; dsRNA; Genome; RNA interference; Red flour beetle; Tribolium castaneum
MeSH headings : ATP-Binding Cassette Transporters / deficiency; ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Animals; Eye / metabolism; Female; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Genomics; Insect Proteins / deficiency; Insect Proteins / genetics; Insect Proteins / metabolism; Larva / anatomy & histology; Larva / genetics; Larva / growth & development; Larva / metabolism; Lipid Metabolism / genetics; Male; Phenotype; Pigmentation / genetics; RNA Interference; Tribolium / anatomy & histology; Tribolium / genetics; Tribolium / growth & development; Tribolium / metabolism
TL;DR: The results from this study provide new insights into the physiological function of ABC transporters in T. castaneum, and may help to establish new target sites for insect control. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Genetic Structure of Tribolium castaneum (Coleoptera: Tenebrionidae) Populations in Mills

Environmental Entomology, 41(1), 188–199.

By: A. Semeao*, J. Campbell*, R. Beeman*, M. Lorenzen*, R. Whitworth* & P. Sloderbeck*

author keywords: Tribolium castaneum; genetic structure; F-ST; genetic fingerprinting; bottleneck
MeSH headings : Animal Migration; Animals; Flour; Genetic Variation; Insect Control / methods; Oryza; Polymerase Chain Reaction; Population Dynamics; Puerto Rico; Tribolium / genetics; Triticum; United States
TL;DR: Evidence is provided that populations of T. castaneum collected from mills show spatial genetic structure, but the poor ability to assign individuals to source populations and lack of isolation by distance suggest greater levels of gene flow than predicted originally. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2012 conference paper

Genetic structure of Tribolium castaneum (Coleoptera: Tenebrionidae) populations in mills

Presented at the 10th International Working Conference on Stored Product Protection, Estoril, Portugal.

By: A. Semeao, J. Campbell, R. Beeman, R. Whitworth, P. Sloderbeck & M. Lorenzen

Event: 10th International Working Conference on Stored Product Protection at Estoril, Portugal on June 27 - July 2, 2010

Source: NC State University Libraries
Added: March 26, 2021

2012 journal article

Transcriptome Profiling of the Intoxication Response of Tenebrio molitor Larvae to Bacillus thuringiensis Cry3Aa Protoxin

PLoS ONE, 7(4), e34624.

By: B. Oppert*, S. Dowd, P. Bouffard*, L. Li*, A. Conesa*, M. Lorenzen n, M. Toutges*, J. Marshall* ...

Ed(s): S. Palli

MeSH headings : Administration, Oral; Animals; Bacillus thuringiensis Toxins; Bacterial Proteins / administration & dosage; Bacterial Proteins / toxicity; Base Sequence; Biosynthetic Pathways / drug effects; DNA, Complementary / genetics; Endotoxins / administration & dosage; Endotoxins / toxicity; Energy Metabolism / drug effects; Gene Expression Profiling; Hemolysin Proteins / administration & dosage; Hemolysin Proteins / toxicity; Larva / drug effects; Larva / metabolism; Microarray Analysis; Molecular Sequence Data; Sequence Analysis, DNA; Tenebrio / drug effects; Tenebrio / genetics; Tenebrio / metabolism; Time Factors; Transcriptome / drug effects
TL;DR: The data suggest that T. molitor larvae mount a complex response to Cry3Aa during the initial 24 h of intoxication, and the methods in this study are useful for comparative analyses in organisms lacking a sequenced genome. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2011 chapter

RNAi in Agriculturally-Important Arthropods

In P. Grabowski (Ed.), RNA Processing (pp. 157–180).

By: K. Aronstein*, B. Oppert* & M. Lorenzen

Ed(s): P. Grabowski

TL;DR: The aim in this chapter is to provide an overview of the profound knowledge accumulated in recent years from invertebrate RNAi studies, but with a focus on agriculturally important arthropods, and the latest achievements in basic honey bee research. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 28, 2021

2010 journal article

RNA Interference as a Method for Target-Site Screening in the Western Corn Rootworm, Diabrotica Virgifera Virgifera

Journal of Insect Science, 10(162), 1–16.

By: A. Alves*, M. Lorenzen*, R. Beeman*, J. Foster* & B. Siegfried*

MeSH headings : Amino Acid Sequence; Animals; Chitin Synthase / genetics; Cloning, Molecular; Coleoptera / genetics; DNA Primers / genetics; Insect Control / methods; Laccase / genetics; Larva; Molecular Sequence Data; Phenotype; RNA Interference; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment
TL;DR: Results indicate that RNAi-induced silencing of D. v. vergifera genes provides a powerful tool for identifying potential insecticide targets. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2009 journal article

A NovelTenebrio molitorCadherin Is a Functional Receptor forBacillus thuringiensisCry3Aa Toxin

Journal of Biological Chemistry, 284(27), 18401–18410.

MeSH headings : Amino Acid Sequence; Animals; Bacillus thuringiensis / metabolism; Bacillus thuringiensis Toxins; Bacterial Proteins / metabolism; Bacterial Proteins / toxicity; Base Sequence; Cadherins / chemistry; Cadherins / genetics; Cadherins / metabolism; Cloning, Molecular; Endotoxins / metabolism; Endotoxins / toxicity; Hemolysin Proteins / metabolism; Hemolysin Proteins / toxicity; Insect Proteins / chemistry; Insect Proteins / genetics; Insect Proteins / metabolism; Larva / genetics; Larva / microbiology; Molecular Sequence Data; Phylogeny; Protein Binding / physiology; Protein Structure, Tertiary; RNA, Small Interfering; Tenebrio / genetics; Tenebrio / growth & development; Tenebrio / microbiology
TL;DR: The functional role of TmCad1 as a Cry3Aa receptor in T. molitor larvae is demonstrated and similarities between the mode of action of Cry toxins in Lepidoptera and Coleoptera are revealed. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: January 24, 2021

2009 journal article

BeetleBase in 2010: revisions to provide comprehensive genomic information for Tribolium castaneum

Nucleic Acids Research, 38(suppl_1), D437–D442.

MeSH headings : Animals; Computational Biology / methods; Computational Biology / trends; Databases, Genetic; Databases, Nucleic Acid; Databases, Protein; Expressed Sequence Tags; Genome; Genomics; Information Storage and Retrieval / methods; Internet; Models, Genetic; Protein Structure, Tertiary; Software; Tribolium / genetics
TL;DR: BeetleBase has been updated to provide more comprehensive genomic information for the red flour beetle Tribolium castaneum, and the BLAST and BLAT search tools have been integrated with the GMOD tools. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2009 journal article

Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps

BMC Biology, 7(1), 73.

By: J. Trauner*, J. Schinko, M. Lorenzen, T. Shippy*, E. Wimmer, R. Beeman, M. Klingler*, G. Bucher, S. Brown*

MeSH headings : Animals; Chromosome Mapping; Crosses, Genetic; DNA Transposable Elements / genetics; Embryo Loss / genetics; Embryo, Nonmammalian; Enhancer Elements, Genetic; Genes, Lethal; Genetic Markers; Germ-Line Mutation; Mutagenesis, Insertional / methods; Transposases; Tribolium / genetics
TL;DR: A large-scale transposon mutagenesis screen for red flour beetle lines produced more than 6,500 new piggyBac insertions, which proved to be recessive lethal, semi-lethal, and eight indicated recessive sterility, while 505 showed new enhancer-trap patterns. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2009 journal article

The Red Flour Beetle, Tribolium castaneum (Coleoptera): A Model for Studies of Development and Pest Biology

Cold Spring Harbor Protocols, 2009(8), pdb.emo126-pdb.emo126.

By: S. Brown*, T. Shippy, S. Miller, R. Bolognesi, R. Beeman, M. Lorenzen*, G. Bucher, E. Wimmer, M. Klingler

MeSH headings : Animals; Animals, Genetically Modified; Body Patterning / genetics; Body Patterning / physiology; Drug Resistance; Female; Genomics / methods; Larva / drug effects; Larva / genetics; Larva / growth & development; Male; Models, Biological; Pest Control; Pesticides / toxicity; RNA Interference; Tribolium / genetics; Tribolium / growth & development
TL;DR: Cold Spring Harb Protoc Lorenzen, Gregor Bucher, Ernst A. Wimmer and Martin Klingler Studies of Development and Pest Biology (Coleoptera): A Model for Tribolium castaneum The Red Flour Beetle. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2009 journal article

Tribolium castaneum Larval Gut Transcriptome and Proteome: A Resource for the Study of the Coleopteran Gut

Journal of Proteome Research, 8(8), 3889–3898.

By: K. Morris*, M. Lorenzen*, Y. Hiromasa*, J. Tomich*, C. Oppert*, E. Elpidina*, K. Vinokurov*, J. Jurat-Fuentes*, J. Fabrick*, B. Oppert*

author keywords: Tribolium castaneum; microarray; proteomics; Coleoptera; insect control; insect gut
MeSH headings : Analysis of Variance; Animals; Digestive System / chemistry; Digestive System / metabolism; Electrophoresis, Gel, Two-Dimensional; Gene Expression Profiling / methods; Insect Control; Insect Proteins / analysis; Insect Proteins / genetics; Insect Proteins / metabolism; Larva / chemistry; Models, Biological; Oligonucleotide Array Sequence Analysis; Peptide Fragments / analysis; Proteome / analysis; Proteome / genetics; Proteome / metabolism; Proteomics / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tribolium / chemistry; Tribolium / genetics; Tribolium / metabolism
TL;DR: The recently acquired T. castaneum genome is used to identify T.Castaneum genes and proteins in one of the more critical environmental interfaces of the insect, the larval alimentary tract, and provides the basis for comparative transcriptomic and proteomic studies related to the gut of coleopteran insects. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2008 journal article

Analysis of repetitive DNA distribution patterns in the Tribolium castaneum genome

Genome Biology, 9(3), R61.

By: S. Wang*, M. Lorenzen*, R. Beeman* & S. Brown*

MeSH headings : Animals; DNA Transposable Elements; Evolution, Molecular; Gene Library; Genome, Insect; Heterochromatin / genetics; Recombination, Genetic; Sequence Analysis, DNA; Tandem Repeat Sequences; Tribolium / genetics
TL;DR: This study found that transposable elements and other repetitive DNA accumulate in certain regions in the assembled T. castaneum genome, and several lines of evidence suggest these regions are derived from the large blocks of pericentric heterochromatin in T.Castaneum chromosomes. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2008 journal article

The genome of the model beetle and pest Tribolium castaneum

Nature, 452(7190), 949–955.

By: S. Richards*, R. Gibbs, G. Weinstock*, S. Brown*, R. Denell*, R. Beeman*, G. Bucher*, M. Friedrich ...

MeSH headings : Animals; Base Composition; Body Patterning / genetics; Cytochrome P-450 Enzyme System / genetics; DNA Transposable Elements / genetics; Genes, Insect / genetics; Genome, Insect / genetics; Growth and Development / genetics; Humans; Insecticides / pharmacology; Neurotransmitter Agents / genetics; Oogenesis / genetics; Phylogeny; Proteome / genetics; RNA Interference; Receptors, G-Protein-Coupled / genetics; Receptors, Odorant / genetics; Repetitive Sequences, Nucleic Acid / genetics; Taste / genetics; Telomere / genetics; Tribolium / classification; Tribolium / embryology; Tribolium / genetics; Tribolium / physiology; Vision, Ocular / genetics
TL;DR: Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model organism for the study of generalized insect development, and an important pest of stored agricultural products. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2008 journal article

The maternal-effect, selfish genetic element Medea is associated with a composite Tc1 transposon

Proceedings of the National Academy of Sciences, 105(29), 10085–10089.

By: M. Lorenzen*, A. Gnirke*, J. Margolis*, J. Garnes*, M. Campbell*, J. Stuart*, R. Aggarwal*, S. Richards*, Y. Park*, R. Beeman*

author keywords: postzygotic; selfish gene; Tribolium; gene driver
MeSH headings : Animals; Chromosome Mapping; Chromosomes, Artificial, Bacterial / genetics; Cloning, Molecular; DNA Transposable Elements / genetics; Female; Gene Dosage; Genes, Insect; Genes, Lethal; Male; Molecular Sequence Data; Mutation; Phylogeny; Repetitive Sequences, Nucleic Acid; Tribolium / genetics; Zygote
TL;DR: Sequence comparisons suggest that the current distribution of Medea1 reflects global emanation after a single transpositional event in recent evolutionary time and could provide a useful vehicle for driving desirable genes into populations. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2008 journal article

Tubulin superfamily genes in Tribolium castaneum and the use of a Tubulin promoter to drive transgene expression

Insect Biochemistry and Molecular Biology, 38(8), 749–755.

By: K. Siebert*, M. Lorenzen*, S. Brown*, Y. Park* & R. Beeman*

author keywords: Tribolium; Tubulin; promoter; PiggyBac; EGFP; vermilion; transgene
MeSH headings : Animals; Base Sequence; Cloning, Molecular; Insect Proteins / genetics; Molecular Sequence Data; Multigene Family; Mutation; Phenotype; Promoter Regions, Genetic; Transgenes; Tribolium / genetics; Tubulin / genetics
TL;DR: 12 Tubulin family members from the genome sequence of the red flour beetle are identified and the promoter from one of these is used to drive constitutive expression of a transgene. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2007 journal article

Analysis of transcriptome data in the red flour beetle, Tribolium castaneum

Insect Biochemistry and Molecular Biology, 38(4), 380–386.

By: Y. Park*, J. Aikins*, L. Wang*, R. Beeman, B. Oppert, J. Lord, S. Brown*, M. Lorenzen ...

author keywords: Coleoptera; EST; GLEAN; gene model
MeSH headings : Animals; Databases, Nucleic Acid; Expressed Sequence Tags; Gene Expression Profiling; Gene Library; Tribolium / genetics; Tribolium / metabolism
TL;DR: All available expressed sequence tag (EST) data is compiled and analyzed to facilitate accurate annotation and detailed functional analysis of this genome, suggesting that a considerable number of transcribed sequences were missed by the gene prediction programs or were removed by GLEAN. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2007 journal article

piggyBac-based insertional mutagenesis in Tribolium castaneum using donor/helper hybrids

Insect Molecular Biology, 16(3), 265–275.

By: M. Lorenzen*, T. Kimzey, T. Shippy, S. Brown, R. Denell & R. Beeman

author keywords: piggyBac; Minos; germline transformation; EGFP; enhancer trap
MeSH headings : Actins / genetics; Animals; Base Sequence; Chromosome Mapping; Crosses, Genetic; DNA Footprinting; DNA Transposable Elements / genetics; Green Fluorescent Proteins / genetics; Molecular Sequence Data; Mutagenesis, Insertional / methods; Phenotype; Sequence Analysis, DNA; Transposases; Tribolium / genetics
TL;DR: The jumpstarter system described herein makes genome‐wide, saturation insertional mutagenesis a realistic goal in this coleopteran species. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2006 journal article

Insights into social insects from the genome of the honeybee Apis mellifera

Nature, 443(7114), 931–949.

Marce Lorenzen

MeSH headings : Animals; Base Composition; Bees / genetics; Bees / growth & development; Bees / physiology; Behavior, Animal; DNA Transposable Elements / genetics; Evolution, Molecular; Female; Gene Expression Regulation / genetics; Genes, Insect / genetics; Genome, Insect / genetics; Genomics; Immunity / genetics; Male; Molecular Sequence Data; Phylogeny; Physical Chromosome Mapping; Proteome / genetics; Reproduction / genetics; Signal Transduction / genetics; Social Behavior; Telomere / genetics
TL;DR: The genome sequence of the honeybee Apis mellifera is reported, suggesting a novel African origin for the species A. melliferA and insights into whether Africanized bees spread throughout the New World via hybridization or displacement. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science; OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2005 journal article

Genetic Linkage Maps of the Red Flour Beetle, Tribolium castaneum, Based on Bacterial Artificial Chromosomes and Expressed Sequence Tags

Genetics, 170(2), 741–747.

By: M. Lorenzen*, Z. Doyungan*, J. Savard*, K. Snow*, L. Crumly*, T. Shippy*, J. Stuart*, S. Brown*, R. Beeman*

MeSH headings : Animals; Chromosome Mapping / methods; Chromosomes, Artificial, Bacterial; Crosses, Genetic; DNA / metabolism; DNA Transposable Elements; DNA, Complementary / metabolism; Databases, Genetic; Expressed Sequence Tags; Female; Genetic Linkage; Internet; Male; Microsatellite Repeats; Models, Genetic; Molecular Sequence Data; Nucleic Acid Heteroduplexes; Physical Chromosome Mapping; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Single-Stranded Conformational; Species Specificity; Tribolium / genetics
TL;DR: A genetic linkage map was constructed in a backcross family of the red flour beetle, Tribolium castaneum, based largely on sequences from bacterial artificial chromosome ends and untranslated regions from random cDNA's, providing a framework for positional cloning and a scaffold for integration of the emerging physical map and genome sequence assembly. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2005 journal article

The Tribolium chitin synthase genes TcCHS1 and TcCHS2 are specialized for synthesis of epidermal cuticle and midgut peritrophic matrix

Insect Molecular Biology, 14(5), 453–463.

author keywords: Tribolium castaneum; chitin synthase; RNAi; peritrophic matrix; cuticle
MeSH headings : Animals; Base Sequence; Chitin / biosynthesis; Chitin Synthase / biosynthesis; Chitin Synthase / genetics; Epidermis / enzymology; Epidermis / growth & development; Gastrointestinal Tract / enzymology; Gastrointestinal Tract / growth & development; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Silencing; Insect Proteins / genetics; Insect Proteins / metabolism; Larva / metabolism; Molting / physiology; Phenotype; Pupa / metabolism; RNA, Messenger / metabolism; Tissue Distribution; Tribolium / embryology; Tribolium / enzymology; Tribolium / genetics
TL;DR: Functional analysis of the two chitin synthase genes, Tc CHS1 and TcCHS2, in the red flour beetle, Tribolium castaneum, revealed unique and complementary roles for each gene. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2004 journal article

DIRS retroelements in arthropods: identification of the recently active TcDirs1 element in the red flour beetle Tribolium castaneum

Molecular Genetics and Genomics, 272(1), 47–56.

By: T. Goodwin*, R. Poulter*, M. Lorenzen* & R. Beeman*

author keywords: insect; transposable element; tyrosine recombinase
MeSH headings : Amino Acid Sequence; Animals; Base Sequence; Bees / genetics; Molecular Sequence Data; Phylogeny; Recombination, Genetic; Retroelements / genetics; Sea Urchins / genetics; Sequence Analysis, DNA; Tribolium / genetics
TL;DR: The first DIRS retroelement to be identified in an arthropod was found in the red flour beetle Tribolium castaneum (Coleoptera), and TcDirs1 elements exhibit several features indicative of recent activity, such as intact coding regions, a high level of sequence similarity between distinct elements and polymorphic insertion sites. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2003 journal article

piggyBac-mediated germline transformation in the beetle Tribolium castaneum

Insect Molecular Biology, 12(5), 433–440.

By: M. Lorenzen*, A. Berghammer, S. Brown*, R. Denell*, M. Klingler & R. Beeman

author keywords: piggyBac; germline transformation; EGFP; enhancer trap; Tribolium castaneum
MeSH headings : Animals; Base Sequence; Blotting, Southern; Chromosome Mapping; DNA Transposable Elements / genetics; Gene Expression Profiling; Microinjections; Molecular Sequence Data; Plasmids; Transformation, Genetic; Tribolium / genetics
TL;DR: It is demonstrated that a primary insertion of a non‐autonomous element can be efficiently remobilized to non‐homologous chromosomes by injection of an immobile helper element into embryos harbouring the primary insertion, suggesting potential for more sophisticated methods of piggyBac‐mediated genome manipulation. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

2002 journal article

Cloning and characterization of the Tribolium castaneum eye-color genes encoding tryptophan oxygenase and kynurenine 3-monooxygenase

Genetics, 160(1), 225–234.

By: M. Lorenzen*, S. Brown*, R. Denell* & R. Beeman*

MeSH headings : Animals; Cloning, Molecular; Drosophila Proteins; Eye Color / genetics; Eye Proteins; Genes, Insect; Humans; Insect Proteins; Kynurenine 3-Monooxygenase; Larva / genetics; Mixed Function Oxygenases / genetics; Molecular Sequence Data; Mutation; Sequence Analysis, DNA; Sequence Homology; Transformation, Genetic; Tribolium / enzymology; Tribolium / genetics; Tryptophan Oxygenase / genetics
Sources: NC State University Libraries, NC State University Libraries
Added: March 26, 2021

2002 journal article

Transgene expression from the Tribolium castaneum Polyubiquitin promoter

Insect Molecular Biology, 11(5), 399–407.

By: M. Lorenzen*, S. Brown*, R. Denell* & R. Beeman*

MeSH headings : Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Complementary; Gene Expression; Genes, Insect; Molecular Sequence Data; Mutagenesis; Phenotype; Polyubiquitin / genetics; Promoter Regions, Genetic; Transgenes; Tribolium / genetics
TL;DR: The cloning and characterization of the Tribolium castaneum Polyubiquitin (TcPUb) gene is reported, and germline transformants were easily recognized by rescue of eye pigmentation, illustrating the potential of the Tc PUb promoter for use in driving transgene expression. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020

1999 journal article

Using RNAi to investigate orthologous homeotic gene function during development of distantly related insects

Evolution and Development, 1(1), 11–15.

By: S. Brown*, J. Mahaffey n, M. Lorenzen*, R. Denell* & J. Mahaffey n

MeSH headings : Animals; Drosophila / embryology; Drosophila / genetics; Drosophila / growth & development; Genes, Homeobox; Mutation; RNA, Double-Stranded / physiology
TL;DR: It is shown that RNA interference can be used to phenocopy mutations of the Deformed orthologues in Drosophila and Tribolium and the possible use of this technique is discussed for comparisons of developmental mechanisms in organisms with differing ontogenies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

Employment

Updated: May 25th, 2023 12:08

2009 - present

NC State University Raleigh, NC, US
Professor Entomology

Education

Updated: May 2nd, 2016 12:36

1994 - 2000

Kansas State University Manhattan, KS, US
PhD Biology

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