@article{whitehead_petritz_doerr_stoskopf_harrison_2020, title={Biochemical Effects of Routine Gonadectomy on Blood of Domestic Ferrets (Mustela putorius furo)}, volume={59}, ISSN={["1559-6109"]}, DOI={10.30802/AALAS-JAALAS-19-000173}, abstractNote={We studied domestic ferrets (Mustela putorius furo) to evaluate the physiologic effects of routine surgery. Standard plasma biochemistry panels and 1H-NMR spectroscopy of heparinized whole blood were performed on samples taken 24 h prior to and immediately after surgery from female and male ferrets undergoing routine gonadectomy. Increases in plasma glucose, phosphorus, potassium, and creatine kinase concentrations associated with the duration of surgery were identified on plasma biochemistry panels. Whole-blood NMR spectra allowed us to identify 42 metabolites and one drug residue. Variations between pre- and postoperative metabolite concentrations were most pronounced for female ferrets, which underwent moreprolonged surgery than males. Affected metabolites included organic acids and osmolytes (betaine, methylmalonate, d-lactate), fatty acids and lipids (2-hydroxy-3-methylbutyric acid), and amino acid groups (acetylglycine, alloisoleucine, leucine, and isoleucine). These findings indicate that 1H-NMR spectroscopy of whole blood provides insight into metabolic perturbations in domestic ferrets undergoing surgery that are not detected in routine clinical chemistry panels.}, number={5}, journal={JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE}, author={Whitehead, Michelle C. and Petritz, Olivia A. and Doerr, Mary and Stoskopf, Michael K. and Harrison, Tara M.}, year={2020}, month={Sep}, pages={567–574} }
 @article{doerr_stoskopf_2019, title={EVALUATION OF EUTHANASIA OF MOON JELLYFISH (AURELIA AURITA) USING SIMPLE SALT SOLUTIONS}, volume={50}, ISSN={["1937-2825"]}, DOI={10.1638/2018-01510}, abstractNote={Abstract: Immersion euthanasia methods reported over the most recent decades for aquatic invertebrates use organic alcohols or halogenated hydrocarbons that can interfere with nuclear magnetic resonance (NMR) analysis. A rolling study design evaluated potassium chloride (KCl), magnesium chloride (MgCl2), and magnesium sulfate (MgSO4) as potential ion-based euthanasia methods for moon jellyfish (Aurelia aurita) destined for metabolomic analysis by NMR spectroscopy. Death was defined as the cessation of autonomous bell pulsing and response to external stimulus. MgCl2 applied at a dose of 142 g/L provided euthanasia within 32 sec of applications without the untoward effects observed with the other two salts. Euthanasia with KCl at the doses tested was associated with abnormal behavior and tissue degradation during dissection. MgSO4 at the doses tested resulted in abnormal behavior and failed to provide rapid euthanasia.}, number={1}, journal={JOURNAL OF ZOO AND WILDLIFE MEDICINE}, author={Doerr, Mary and Stoskopf, Michael K.}, year={2019}, month={Mar}, pages={123–126} }