@article{enomoto_castro_hash_thomson_nakanishi-hester_perry_aker_haupt_opperman_roe_et al._2024, title={Prevalence of radiographic appendicular osteoarthritis and associated clinical signs in young dogs}, volume={14}, ISSN={["2045-2322"]}, DOI={10.1038/s41598-024-52324-9}, abstractNote={Abstract}, number={1}, journal={SCIENTIFIC REPORTS}, author={Enomoto, Masataka and Castro, Nicholas and Hash, Jonathan and Thomson, Andrea and Nakanishi-Hester, Aoi and Perry, Erin and Aker, Savannah and Haupt, Emily and Opperman, Logan and Roe, Simon and et al.}, year={2024}, month={Feb} }
 @article{wu_nichols_foster_martin_dieffenderfer_enomoto_lascelles_russenberger_brenninkmeyer_bozkurt_et al._2023, title={An Exploration of Machine Learning Methods for Gait Analysis of Potential Guide Dogs}, url={https://doi.org/10.1145/3637882.3637883}, DOI={10.1145/3637882.3637883}, abstractNote={Gait analysis is important for guide dog organizations, as ideal guide dogs have a smooth and efficient gait, where they can also easily shift between and maintain various gaits. Gait quality and natural traveling speed are two of the multiple factors important in matching a guide dog to its visually impaired handler. Gait evaluation typically includes subjective visual observation of the dog or objective assessments obtained from special-designed equipment. Guide dog organizations need a method to easily collect and analyze objective data of gait information. In this work, we explored how various machine learning models could learn and analyze gait patterns from inertial measurements data that were collected during two different data collection experiments using a wearable sensor device. We also evaluated how well each machine learning model could generalize behavior patterns from various dogs under different environments. Additionally, we compared how sensor placement locations could affect gait prediction performance by attaching the sensor device to the dog’s neck and back area respectively. The tested machine learning models were able to classify different gaits in the range of 42% to 91% in terms of accuracy, and predict various gait parameters with an error rate ranging from 14% to 29% depending on the setup. Furthermore, we also observed that using behavior data collected from the neck region contains more movement information than the back area. By performing a cross-dataset generalization test on the machine learning models, we found that even with performance drop, the models were able to learn gait-specific behavior patterns that are generalizable for different dogs. Although the results were preliminary, the proposed gait analysis exploration still showed promising potential for studying behavior patterns of candidate guide dogs.}, journal={TENTH INTERNATIONAL CONFERENCE ON ANIMAL-COMPUTER INTERACTION, ACI 2023}, author={Wu, Yifan and Nichols, Colt and Foster, Marc and Martin, Devon and Dieffenderfer, James and Enomoto, Masataka and Lascelles, B. Duncan X. and Russenberger, Jane and Brenninkmeyer, Gerald and Bozkurt, Alper and et al.}, year={2023} }
 @article{nakanishi_lascelles_allen_case_gearing_enomoto_2022, title={A Pilot, Open-Label Study to Evaluate the Efficacy of Intra-Articular Administration of a Caninized TNF Receptor Fc Fusion Protein as a Treatment for Osteoarthritis-Associated Joint Pain}, volume={9}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2022.836709}, abstractNote={Tumor necrosis factor-α (TNF-α) is a potential target for osteoarthritis (OA) treatment. In several recent clinical studies in human OA, anti-TNF-α therapy showed promising results; however, these were open-label and based on patient-reported outcome measures. In this study, we developed a caninized TNF-α receptor-Fc (caTNFR-Fc) fusion protein and conducted a non-randomized, open-label, pilot study in dogs with OA using objectively measured ground reaction forces and activity. The aims of the study were to assess the efficacy of the intra-articular (IA) injection of the caTNFR-Fc fusion protein as a treatment for OA pain, and additionally to evaluate TNF concentrations in synovial fluid (SF) between joints with/without OA in dogs. Dogs (n = 12) with single-limb lameness due to single joint appendicular OA were recruited. All dogs received caTNFR-Fc fusion protein injection into the affected joint under sedation. Objective kinetic gait analysis using force plate was performed prior to (baseline), and at 14- and 28-days following treatment. Additionally, SF samples were collected from OA joints (n = 69) and non-OA joints (n = 79) in a different cohort of dogs and TNF-α were measured using enzyme-linked immunosorbent assay. No significant treatment effects on the limb use, activity, and the questionnaire were found. The concentration of TNF-α was significantly higher in OA joints than in healthy joints (p = 0.0019), but TNF-α was detected in only 10/69 OA samples. The IA injection of caTNFR-Fc fusion protein provided no benefit in terms of objective limb use and activity data in dogs with OA in this pilot study. Although the SF concentration of TNF-α was significantly higher in OA joints, few OA joints had measurable TNF-α. Collectively, the data indicate TNF-α may not be a good therapeutic target in canine OA.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Nakanishi, Aoi and Lascelles, B. Duncan X. and Allen, Julie and Case, Beth and Gearing, David and Enomoto, Masataka}, year={2022}, month={Jun} }
 @article{ortiz_belda_hash_enomoto_robertson_lascelles_2022, title={Initial exploration of the discriminatory ability of the PetPace collar to detect differences in activity and physiological variables between healthy and osteoarthritic dogs}, volume={3}, ISSN={["2673-561X"]}, url={http://dx.doi.org/10.3389/fpain.2022.949877}, DOI={10.3389/fpain.2022.949877}, abstractNote={BackgroundAccelerometry has been used to evaluate activity in dogs with osteoarthritis (OA) pain, especially in relation to effect of treatment; however no studies have compared accelerometry-measured activity in dogs with OA-pain and healthy dogs. The aims of this study were to (1) compare activity output from the PetPace collar with the validated Actical monitor and (2) determine if PetPace collar outputs (overall activity, activity levels, body position, and vital signs) differed between healthy dogs and dogs with OA-pain.}, journal={FRONTIERS IN PAIN RESEARCH}, publisher={Frontiers Media SA}, author={Ortiz, Avery Rowlison and Belda, Beatriz and Hash, Jon and Enomoto, Masataka and Robertson, James and Lascelles, B. Duncan X.}, year={2022}, month={Sep} }
 @article{wu_holder_foster_williams_enomoto_lascelles_bozkurt_roberts_2022, title={Spatial and Temporal Analytic Pipeline for Evaluation of Potential Guide Dogs Using Location and Behavior Data}, url={https://doi.org/10.1145/3565995.3566033}, DOI={10.1145/3565995.3566033}, abstractNote={Training guide dogs for visually-impaired people is a resource-consuming task for guide dog schools. This task is further complicated by a dearth of capabilities to objectively measure and analyze candidate guide dogs’ temperaments as they are placed with volunteer raisers away from guide dog schools for months during the raising process. In this work, we demonstrate a preliminary data analysis workflow that is able to provide detailed information about candidate guide dogs’ day to day physical exercise levels and gait activities using objective environmental and behavioral data collected from a wearable collar-based Internet of Things device. We trained and tested machine learning models to analyze different gait types including walking, pacing, trotting and mixture of walk and trot. By analyzing data both spatially and temporally, a location and behavior summary for candidate dogs is generated to provide insight for guide dog training experts, so that they can more accurately and comprehensively evaluate the future success of the candidate. The preliminary analysis revealed movement patterns for different location types which reflected the behaviors of candidate guide dogs.}, journal={NINTH INTERNATIONAL CONFERENCE ON ANIMAL-COMPUTER INTERACTION, ACI 2022}, author={Wu, Yifan and Holder, Timothy and Foster, Marc and Williams, Evan and Enomoto, Masataka and Lascelles, B. Duncan X. and Bozkurt, Alper and Roberts, David L.}, year={2022} }
 @article{estes_enomoto_moutos_carson_toth_eggert_stallrich_willard_veis_little_et al._2021, title={Biological resurfacing in a canine model of hip osteoarthritis}, volume={7}, ISSN={["2375-2548"]}, url={https://doi.org/10.1126/sciadv.abi5918}, DOI={10.1126/sciadv.abi5918}, abstractNote={A 3D woven scaffold seeded with MSCs was used to resurface the canine hip, reducing pain and improving joint function.}, number={38}, journal={SCIENCE ADVANCES}, publisher={American Association for the Advancement of Science (AAAS)}, author={Estes, Bradley T. and Enomoto, Masataka and Moutos, Franklin T. and Carson, Megan A. and Toth, Jeffrey M. and Eggert, Peter and Stallrich, Jonathan and Willard, Vincent P. and Veis, Deborah J. and Little, Dianne and et al.}, year={2021}, month={Sep} }
 @article{enomoto_baines_roe_marcellin-little_lascelles_2021, title={Defining the rate of, and factors influencing, radiographic progression of osteoarthritis of the canine hip joint}, volume={189}, ISSN={["2042-7670"]}, url={https://doi.org/10.1002/vetr.516}, DOI={10.1002/vetr.516}, abstractNote={Abstract}, number={10}, journal={VETERINARY RECORD}, publisher={Wiley}, author={Enomoto, Masataka and Baines, Elizabeth A. and Roe, Simon C. and Marcellin-Little, Denis J. and Lascelles, B. Duncan X.}, year={2021}, month={Jun} }
 @article{enomoto_lascelles_robertson_gruen_2021, title={Refinement of the Feline Musculoskeletal Pain Index (FMPI) and development of the short-form FMPI}, volume={24}, ISSN={["1532-2750"]}, url={https://doi.org/10.1177/1098612X211011984}, DOI={10.1177/1098612X211011984}, abstractNote={Objectives The aim of this study was to investigate the reliability and responsiveness of the Feline Musculoskeletal Pain Index (FMPI) using the collective results of multiple clinical studies and iteratively refine the FMPI for future use. }, number={2}, journal={JOURNAL OF FELINE MEDICINE AND SURGERY}, publisher={SAGE Publications}, author={Enomoto, Masataka and Lascelles, B. Duncan X. and Robertson, James B. and Gruen, Margaret E.}, year={2021}, month={May} }
 @article{correlation of artemin and gfrα3 with osteoarthritis pain: early evidence from naturally occurring osteoarthritis-associated chronic pain in dogs_2020, url={http://dx.doi.org/10.3389/fnins.2020.00077}, DOI={10.3389/fnins.2020.00077}, abstractNote={Arthritis, including osteoarthritis (OA) and other musculoskeletal-associated pain, is a worldwide problem, however, effective drug options are limited. Several receptors, neurotransmitters, and endogenous mediators have been identified in rodent models, but the relevance of these molecules in disease-associated pain is not always clear. Artemin, a neurotrophic factor, and its receptor, glial-derived neurotrophic factor (GDNF) family receptor alpha-3 (GFRα3), have been identified as involved in pain in rodents. Their role in OA-associated pain is unknown. To explore a possible association, we analyzed tissue from naturally occurring OA in dogs to characterize the correlation with chronic pain. We used behavioral assessment, objective measures of limb use, and molecular tools to identify whether artemin and GFRα3 might be associated with OA pain. Our results using banked tissue from well-phenotyped dogs indicates that artemin/GFRα3 may play an important, and hitherto unrecognized, role in chronic OA-associated pain. Elevated serum levels of artemin from osteoarthritic humans compared to healthy individuals suggest translational relevance. Our data provide compelling evidence that the artemin/GFRα3 signaling pathway may be important in OA pain in both non-humans and humans and may ultimately lead to novel therapeutics.}, journal={Frontiers in Neuroscience}, year={2020}, month={Feb} }
 @article{enomoto_lascelles_gruen_2020, title={Development of a checklist for the detection of degenerative joint disease-associated pain in cats}, volume={22}, url={https://doi.org/10.1177/1098612X20907424}, DOI={10.1177/1098612X20907424}, abstractNote={Objectives The aim of this study was to develop an evidence-based, clinically expedient checklist to identify cats likely to have degenerative joint disease (DJD)-associated pain. }, number={12}, journal={Journal of Feline Medicine and Surgery}, publisher={SAGE Publications}, author={Enomoto, Masataka and Lascelles, B Duncan X and Gruen, Margaret E}, year={2020}, month={Dec}, pages={1137–1147} }
 @article{enomoto_mantyh_murrell_innes_lascelles_2019, title={Anti-nerve growth factor monoclonal antibodies for the control of pain in dogs and cats}, volume={184}, DOI={10.1136/vr.104590}, abstractNote={Nerve growth factor (NGF) is essential for the survival of sensory and sympathetic neurons during development. However, in the adult, NGF and its interaction with tropomyosin receptor kinase A receptor (TrkA) has been found to play a critical role in nociception and nervous system plasticity in pain conditions. Thus, various monoclonal antibody (mAb) therapies targeting this pathway have been investigated in the development of new pharmacotherapies for chronic pain. Although none of the mAbs against NGF are yet approved for use in humans, they look very promising for the effective control of pain. Recently, species‐specific anti‐NGF mAbs for the management of osteoarthritis (OA)‐associated pain in dogs and cats has been developed, and early clinical trials have been conducted. Anti‐NGF therapy looks to be both very effective and very promising as a novel therapy against chronic pain in dogs and cats. This review outlines the mechanism of action of NGF, the role of NGF in osteoarthritis, research in rodent OA models and the current status of the development of anti‐NGF mAbs in humans. Furthermore, we describe and discuss the recent development of species‐specific anti‐NGF mAbs for the treatment of OA‐associated pain in veterinary medicine.}, number={1}, journal={Vet Rec}, publisher={BMJ}, author={Enomoto, M. and Mantyh, P.W. and Murrell, J. and Innes, J.F. and Lascelles, B.D.X.}, year={2019}, month={Jan}, pages={14} }
 @article{pet dogs with subclinical acute radiodermatitis experience widespread somatosensory sensitization_2019, url={http://dx.doi.org/10.1667/rr15468.1}, DOI={10.1667/rr15468.1}, abstractNote={Radiation-induced dermatitis (RID) is a common and painful complication of radiotherapy. When severe, radiation-associated pain (RAP) can reduce the efficacy of radiotherapy by limiting the radiation dose given, and/or necessitating breaks in treatment. Current RAP mitigation strategies are of limited efficacy. Our long-term goal is to develop a comparative oncology model, in which novel analgesic interventions for RAP can be evaluated. The aim of this study was to validate quantitative end points indicative of RAP in pet dogs with subclinical and low-grade RID. Extremity soft tissue sarcomas were treated with post-operative irradiation (54 Gy in 18 fractions). Visual toxicity scores, questionnaire-based pain instruments and objective algometry [mechanical quantitative sensory testing (mQST)], were evaluated regularly. Breed-matched control populations were also evaluated to address the effect of potential confounders. Skin biopsies from within the irradiated field were collected at baseline and after 24 Gy irradiation, for analysis of pain-related genes using the nanoString nCounter platform. Relative to control populations, mechanical thresholds decreased in irradiated test subjects as the total radiation dose increased, with the most pronounced effect at the irradiated site. This was accompanied by increased mRNA expression of GFRα3, TNFα, TRPV2 and TRPV4. In a separate set of dogs with moderate-to-severe RID, serum concentrations of artemin (the ligand for GFRα3) were elevated relative to controls (P = 0.015). Progressive reduction in mechanical thresholds, both locally and remotely, indicates widespread somatosensory sensitization during radiation treatment. mQST in pet dogs undergoing radiation treatment represents an innovative tool for preclinical evaluation of novel analgesics.}, journal={Radiation Research}, year={2019}, month={Dec} }
 @article{muller_enomoto_buono_steiner_lascelles_2019, title={Placebo-controlled pilot study of the effects of an eggshell membrane-based supplement on mobility and serum biomarkers in dogs with osteoarthritis}, volume={253}, DOI={10.1016/j.tvjl.2019.105379}, abstractNote={Osteoarthritis (OA) is a debilitating disease in dogs. Non-steroidal anti-inflammatory drugs (NSAIDs) are commonly used to treat OA; however, many dogs do not obtain adequate pain relief with an NSAID alone. This pilot study evaluated the systemic anti-inflammatory and mobility enhancing effects of an eggshell membrane-based nutritional supplement in dogs with OA-associated pain and mobility impairment. Twenty-seven dogs with OA-associated pain were enrolled into a randomized, double-masked, placebo-controlled, proof of principle pilot study and received either placebo or an eggshell membrane-based nutritional supplement over a 12-week period. Inflammatory biomarkers (IL-2, IL-6, IL-8, tumor necrosis factor-α, C-reactive protein, S100A12, and N-methylhistamine) were measured at Day 0 and Day 84. Owner questionnaires (CBPI and LOAD) were completed at Day 0, Day 42, and Day 84. Differences between groups over time were calculated. Twenty-two dogs completed the pilot study. Inflammatory biomarker IL-2 decreased in the supplement group, compared to the placebo group. Although small, the difference was statistically significant at an alpha of 0.1 (P = 0.069). LOAD scores were numerically lower in the supplement group, but not significantly different from the placebo group at Day 0. Day 84 LOAD scores were significantly lower in the supplement group compared to the placebo group (P = 0.034). CBPI results did not show the same pattern. The changes in biomarkers and LOAD scores were small, and do not provide definitive evidence of positive effects. However, these pilot results provide a rationale for performing a larger placebo-controlled study of the potential effects of the eggshell membrane-based nutritional supplement.}, journal={The Veterinary Journal}, publisher={Elsevier BV}, author={Muller, C. and Enomoto, M. and Buono, A. and Steiner, J.M. and Lascelles, B.D.X.}, year={2019}, month={Nov}, pages={105379} }
 @article{hunt_goff_jenkins_harris_knowles_lascelles_enomoto_mendl_whay_murrell_et al._2018, title={Electrophysiological characterisation of central sensitisation in canine spontaneous osteoarthritis}, volume={159}, ISSN={["1872-6623"]}, url={https://dx.doi.org/10.1097/j.pain.0000000000001336}, DOI={10.1097/j.pain.0000000000001336}, abstractNote={Abstract}, number={11}, journal={PAIN}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Hunt, James R. and Goff, Megan and Jenkins, Helen and Harris, John and Knowles, Toby G. and Lascelles, B. Duncan X. and Enomoto, Masataka and Mendl, Michael and Whay, Helen R. and Murrell, Joanna C. and et al.}, year={2018}, month={Nov}, pages={2318–2330} }
 @article{belda_enomoto_case_lascelles_2018, title={Initial evaluation of PetPace activity monitor}, volume={237}, url={https://doi.org/10.1016/j.tvjl.2018.05.011}, DOI={10.1016/j.tvjl.2018.05.011}, abstractNote={Mobility is considered a vital component of health and quality of life in humans and companion animals. Wearable devices for pets that can monitor activity and other aspects of health are increasingly being marketed to veterinarians and owners, with claims around their ability to monitor aspects of health. However, there is little scientific evidence to support the validity of these claims. To address this, the objective of this study was to assess the correlation of the activity measurement from the PetPace device compared to activity output from Actigraph and the validated Actical device. Ten client-owned, healthy dogs were used for the study. The three devices were mounted simultaneously on a dedicated collar and activity was recorded during a period of 7 days. There were moderate correlations between the Actical and the PetPace (r2 = 0.59, P = <0.001). There was high correlation between the PetPace and the Actigraph (r2 = 0.85, P = <0.001) and between the Actical and the Actigraph (r2 = 0.72, P = <0.001). If the Actical activity counts were limited under 50,000 per hour, there was strong correlation between the Actical and the PetPace (r2 = 0.71, P = <0.001) and between the Actical and the Actigraph (r2 = 0.86, P = <0.001). PetPace has a moderate correlation with the most validated activity monitor that has been used in veterinary medicine. Its real-time data acquisition, user friendly interface for owners and cost make this device an attractive tool for monitoring activity in dogs. Further studies maybe needed to evaluate its performance, validity and clinical utility in the field.}, journal={Vet J}, publisher={Elsevier BV}, author={Belda, B. and Enomoto, M. and Case, B.C. and Lascelles, B.D.X.}, year={2018}, month={Jul}, pages={63–68} }
 @article{muller_enomoto_steiner_lascelles_2018, title={Randomized Pilot Trial of the Effects of an Egg-Shell Membrane-Based Supplement (Movoflextm) on Mobility and Serum Biomarkers of Inflammation in Dogs with Osteoarthritis}, volume={31}, ISSN={0932-0814 2567-6911}, url={http://dx.doi.org/10.1055/S-0038-1668204}, DOI={10.1055/s-0038-1668204}, abstractNote={Introduction: Despite increasing interest in nutritional supplements, evidence of analgesic efficacy is lacking beyond the omega-3 fatty acids. The purpose of this pilot study was to evaluate the mobility enhancing and systemic anti-inflammatory effects of an eggshell membrane-based nutritional supplement (Movoflex) in dogs with osteoarthritis (OA)-associated pain and mobility impairment.}, number={S 02}, journal={Veterinary and Comparative Orthopaedics and Traumatology}, publisher={Georg Thieme Verlag KG}, author={Muller, Carrie and Enomoto, Masataka and Steiner, Joerg and Lascelles, B.}, year={2018}, month={Jul}, pages={A1–A25} }
 @article{enomoto_lascelles_gerard_2017, title={Defining local nerve blocks for feline distal pelvic limb surgery: a cadaveric study}, volume={19}, ISSN={["1532-2750"]}, url={https://dx.doi.org/10.1177/1098612x17690652}, DOI={10.1177/1098612x17690652}, abstractNote={Objectives Anatomical and methodological detail is lacking regarding local anesthetic peripheral nerve block techniques for distal pelvic limb surgery in cats. The aim of this study was to develop, describe and test nerve block methods based on cadaveric dissections and dye injections. }, number={12}, journal={JOURNAL OF FELINE MEDICINE AND SURGERY}, publisher={SAGE Publications}, author={Enomoto, Masataka and Lascelles, B. Duncan X. and Gerard, Mathew P.}, year={2017}, month={Dec}, pages={1215–1223} }
 @article{enomoto_kigin_bledsoe_slone_hash_smith_lascelles_2017, title={Pilot evaluation of a novel unilateral onychectomy model and efficacy of an extended release buprenorphine product}, volume={13}, journal={BMC Veterinary Research}, author={Enomoto, M. and Kigin, P. D. and Bledsoe, D. and Slone, R. and Hash, J. and Smith, C. E. and Lascelles, B. D. X.}, year={2017} }
 @article{enomoto_lascelles_gerard_2016, title={Defining the local nerve blocks for feline distal thoracic limb surgery: a cadaveric study}, volume={18}, ISSN={["1532-2750"]}, url={https://dx.doi.org/10.1177/1098612x15597967}, DOI={10.1177/1098612x15597967}, abstractNote={Objectives Though controversial, onychectomy remains a commonly performed distal thoracic limb surgical procedure in cats. Peripheral nerve block techniques have been proposed in cats undergoing onychectomy but evidence of efficacy is lacking. Preliminary tests of the described technique using cadavers resulted in incomplete staining of nerves. The aim of this study was to develop nerve block methods based on cadaveric dissections and test these methods with cadaveric dye injections. }, number={10}, journal={JOURNAL OF FELINE MEDICINE AND SURGERY}, publisher={SAGE Publications}, author={Enomoto, Masataka and Lascelles, B. Duncan X. and Gerard, Mathew P.}, year={2016}, month={Oct}, pages={838–845} }
 @article{enomoto_kigin_bledsoe_slone_hash_smith_lascelles_2016, title={Pilot evaluation of a novel unilateral onychectomy model and efficacy of an extended release buprenorphine product}, volume={13}, DOI={10.1186/s12917-017-0943-5}, abstractNote={Non-steroidal anti-inflammatory drugs (NSAIDs), transdermal fentanyl patches, and transmucosal buprenorphine are probably the most commonly used options for providing post-operative analgesia in the early at-home period. However, these require daily administration or are associated with abuse concerns. One of the significant unmet needs in veterinary surgery and pain management is for longer acting opioids for cats to effectively bridge the gap between the in-hospital and at-home recovery periods. A proof of concept study of an extended release formulation of buprenorphine HCL (ER-Bup) was conducted using objective kinetic measures and a unilateral onychectomy model. Using a blinded, randomized, two period crossover design, four cats were allocated to control (saline) or ER-Bup (0.6 mg/kg, subcutaneously [SC]) treatment groups. All animals underwent a unilateral forelimb onychectomy per period with a washout/recovery period in between. Observational pain scores and kinetic data (using a pressure sensitive walkway [PSW]) were collected prior to (baseline) and at intervals for 72 h following surgery. Symmetry indices were derived for kinetic variables (peak vertical force [PVF]; vertical impulse [VI]) of each forelimb for landing following a jump and for walking. A rescue analgesic protocol was in place. Effect of surgery and treatment were evaluated using a mixed model statistical approach. No cats required rescue analgesics based on subjective pain score. ER-Bup had a positive influence on subjective pain scores during the 72 h postsurgery (p = 0.0473). PVF and VI of the operated limb were significantly decreased for both landing (p < 0.0001 and p < 0.0001) and walking (p < 0.0001 and p < 0.0001 respectively) compared to control. ER-Bup resulted in significantly decreased asymmetry in limb use during landing (PVF, p < 0.0001; VI, p < 0.0001) and walking (PVF, p = 0.0002, VI, p < 0.0001). The novel use of data collected following a jump from an elevated platform appeared to provide all desired information and was easier to collect than walking data. This study demonstrates that SC administration of ER-Bup may be an effective analgesic for a 72 h period postoperatively. Furthermore, landing onto a PSW from an elevated perch may be a useful and efficient way to assess analgesics in cats using a unilateral model of limb pain.}, number={1}, journal={BMC Veterinary Research}, publisher={Springer Nature}, author={Enomoto, Masataka and Kigin, Patricia D. and Bledsoe, David and Slone, Robyn and Hash, Jonathan and Smith, Charles E. and Lascelles, B. Duncan X.}, year={2016} }