@article{hall_george_kim_hwang_samberg_monteiro-riviere_narayan_2014, title={Growth of Zircone on Nanoporous Alumina Using Molecular Layer Deposition}, volume={66}, ISSN={["1543-1851"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84898791280&partnerID=MN8TOARS}, DOI={10.1007/s11837-014-0933-z}, number={4}, journal={JOM}, author={Hall, Robert A. and George, Steven M. and Kim, Yeongae and Hwang, Woonbong and Samberg, Meghan E. and Monteiro-Riviere, Nancy A. and Narayan, Roger J.}, year={2014}, month={Apr}, pages={649–653} }
 @article{samberg_mente_he_king_monteiro-riviere_2014, title={In Vitro Biocompatibility and Antibacterial Efficacy of a Degradable Poly(L-lactide-co-epsilon-caprolactone) Copolymer Incorporated with Silver Nanoparticles}, volume={42}, ISSN={["1573-9686"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84904246786&partnerID=MN8TOARS}, DOI={10.1007/s10439-013-0929-9}, abstractNote={Silver nanoparticles (Ag-nps) are currently used as a natural biocide to prevent undesired bacterial growth in clothing, cosmetics and medical products. The objective of the study was to impart antibacterial properties through the incorporation of Ag-nps at increasing concentrations to electrospun degradable 50:50 poly(l-lactide-co-epsilon-caprolactone) scaffolds for skin tissue engineering applications. The biocompatibility of the scaffolds containing Ag-nps was evaluated with human epidermal keratinocytes (HEK); cell viability and proliferation were evaluated using Live/Dead and alamarBlue viability assays following 7 and 14 days of cell culture on the scaffolds. Significant decreases in cell viability and proliferation were noted for the 1.0 mg(Ag) g(scaffold)−1 after 7 and 14 days on Ag-nps scaffolds. After 14 days, scanning electron microscopy revealed a confluent layer of HEK on the surface of the 0.0 and 0.1 mg(Ag) g(scaffold)−1. Both 0.5 and 1.0 mg(Ag) g(scaffold)−1 were capable of inhibiting both Gram positive and negative bacterial strains. Uniaxial tensile tests revealed a significant (p < 0.001) decrease in the modulus of elasticity following Ag-nps incorporation compared to control. These findings suggest that a scaffold containing between 0.5 and 1.0 mg(Ag) g(scaffold)−1 is both biocompatible and antibacterial, and is suitable for skin tissue engineering graft scaffolds.}, number={7}, journal={ANNALS OF BIOMEDICAL ENGINEERING}, author={Samberg, Meghan E. and Mente, Peter and He, Ting and King, Martin W. and Monteiro-Riviere, Nancy A.}, year={2014}, month={Jul}, pages={1482–1493} }
 @article{samberg_tan_monteiro-riviere_orndorff_shirwaiker_2013, title={Biocompatibility analysis of an electrically-activated silver-based antibacterial surface system for medical device applications}, volume={24}, ISSN={["1573-4838"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84876410058&partnerID=MN8TOARS}, DOI={10.1007/s10856-012-4838-5}, number={3}, journal={JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE}, author={Samberg, Meghan E. and Tan, Zhuo and Monteiro-Riviere, Nancy A. and Orndorff, Paul E. and Shirwaiker, Rohan A.}, year={2013}, month={Mar}, pages={755–760} }
 @misc{shirwaiker_samberg_cohen_wysk_monteiro-riviere_2013, title={Nanomaterials and synergistic low-intensity direct current (LIDC) stimulation technology for orthopedic implantable medical devices}, volume={5}, ISSN={["1939-0041"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84876463086&partnerID=MN8TOARS}, DOI={10.1002/wnan.1201}, abstractNote={Abstract Nanomaterials play a significant role in biomedical research and applications because of their unique biological, mechanical, and electrical properties. In recent years, they have been utilized to improve the functionality and reliability of a wide range of implantable medical devices ranging from well‐established orthopedic residual hardware devices (e.g., hip implants) that can repair defects in skeletal systems to emerging tissue engineering scaffolds that can repair or replace organ functions. This review summarizes the applications and efficacies of these nanomaterials that include synthetic or naturally occurring metals, polymers, ceramics, and composites in orthopedic implants, the largest market segment of implantable medical devices. The importance of synergistic engineering techniques that can augment or enhance the performance of nanomaterial applications in orthopedic implants is also discussed, the focus being on a low‐intensity direct electric current (LIDC) stimulation technology to promote the long‐term antibacterial efficacy of oligodynamic metal‐based surfaces by ionization, while potentially accelerating tissue growth and osseointegration. While many nanomaterials have clearly demonstrated their ability to provide more effective implantable medical surfaces, further decisive investigations are necessary before they can translate into medically safe and commercially viable clinical applications. The article concludes with a discussion about some of the critical impending issues with the application of nanomaterials‐based technologies in implantable medical devices, and potential directions to address these. WIREs Nanomed Nanobiotechnol 2013, 5:191–204. doi: 10.1002/wnan.1201 This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Implantable Materials and Surgical Technologies > Nanomaterials and Implants Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement}, number={3}, journal={WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY}, author={Shirwaiker, Rohan A. and Samberg, Meghan E. and Cohen, Paul H. and Wysk, Richard A. and Monteiro-Riviere, Nancy A.}, year={2013}, pages={191–204} }
 @article{monteiro-riviere_samberg_oldenburg_riviere_2013, title={Protein binding modulates the cellular uptake of silver nanoparticles into human cells: Implications for in vitro to in vivo extrapolations?}, volume={220}, ISSN={["1879-3169"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84879479222&partnerID=MN8TOARS}, DOI={10.1016/j.toxlet.2013.04.022}, abstractNote={Nanoparticles (NP) absorbed in the body will come in contact with blood proteins and form NP/protein complexes termed protein coronas, which may modulate NP cellular uptake. This study quantitated human epidermal keratinocyte (HEK) uptake of silver (Ag) NP complexed to different human serum proteins. Prior to HEK dosing, AgNP (20nm and 110nm citrate BioPure™; 40nm and 120nm silica-coated) were preincubated for 2h at 37°C without (control) or with physiological levels of albumin (44mg/ml), IgG (14.5mg/ml) or transferrin (3mg/ml) to form protein-complexed NP. HEK were exposed to the protein incubated AgNP for 3h, rinsed and incubated for 24h, rinsed in buffer and lysed. Ag was assayed by inductively-coupled plasma optical emission spectrometry. Uptake of Ag in HEK was <4.1% of applied dose with proteins suppressing citrate, but not silica coated Ag uptake. IgG exposure dramatically reduced 110nm citrate AgNP uptake. In contrast, greatest uptake of 20nm silica AgNP was seen with IgG, while 110nm silica AgNP showed minimal protein effects. Electron microscopy confirmed cellular uptake of all NP but showed differences in the appearance and agglomeration state of the NP within HEK vacuoles. This work suggests that NP association with different serum proteins, purportedly forming different protein coronas, significantly modulates Ag uptake into HEK compared to native NP uptake, suggesting caution in extrapolating in vitro uptake data to predict behavior in vivo where the nature of the protein corona may determine patterns of cellular uptake, and thus biodistribution, biological activity and toxicity.}, number={3}, journal={TOXICOLOGY LETTERS}, author={Monteiro-Riviere, Nancy A. and Samberg, Meghan E. and Oldenburg, Steven J. and Riviere, Jim E.}, year={2013}, month={Jul}, pages={286–293} }
 @article{samberg_loboa_oldenburg_monteiro-riviere_2012, title={Silver nanoparticles do not influence stem cell differentiation but cause minimal toxicity}, volume={7}, ISSN={["1748-6963"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84868143959&partnerID=MN8TOARS}, DOI={10.2217/nnm.12.18}, abstractNote={Aims: To evaluate the toxicity and cellular uptake of both undifferentiated and differentiated human adipose-derived stem cells (hASCs) exposed to silver nanoparticles (Ag-NPs), and to assess their effect on hASC differentiation. Materials & methods: hASC were exposed to 10- or 20-nm Ag-NPs at concentrations of 0.1, 1.0, 10.0, 50.0 and 100.0 µg/ml either before or after differentiation down the adipogenic or osteogenic pathways. Results: Exposure of hASC to either 10- or 20-nm Ag-NPs resulted in no significant cytotoxicity to hASC, and minimal dose-dependent toxicity to adipogenic and osteogenic cells at 10 µg/ml. Each of the hASC, adipogenic and osteogenic cells showed cellular uptake of both 10- and 20-nm Ag-NPs, without causing significant ultrastructural alterations. Exposure to 10- or 20-nm Ag-NPs did not influence the differentiation of the cells, and at antimicrobial concentrations of Ag-NPs resulted in a minimal decrease in viability. Conclusion: The biocompatibility of Ag-NPs with both undifferentiated and differentiated hASC establishes their suitability for incorporation into tissue-engineered graft scaffolds, for the prevention of bacterial contamination upon implantation. Original submitted 28 September 2011; Revised submitted 15 November 2011; Published online 14 May 2012}, number={8}, journal={NANOMEDICINE}, author={Samberg, Meghan E. and Loboa, Elizabeth G. and Oldenburg, Steven J. and Monteiro-Riviere, Nancy A.}, year={2012}, month={Aug}, pages={1197–1209} }
 @article{samberg_orndorff_monteiro-riviere_2011, title={Antibacterial efficacy of silver nanoparticles of different sizes, surface conditions and synthesis methods}, volume={5}, ISSN={["1743-5404"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000290936000012&KeyUID=WOS:000290936000012}, DOI={10.3109/17435390.2010.525669}, abstractNote={Silver nanoparticles (Ag-nps) are used as a natural biocide to prevent undesired bacterial growth in clothing and cosmetics. The objective of this study was to assess the antibacterial efficacy of Ag-nps of different sizes, surface conditions, and synthesis methods against Escherichia coli, Ag-resistant E. coli, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), and Salmonella sp. Ag-nps samples were synthesized by: Base reduction with unmodified surfaces and used as synthesized ('unwashed'; 20, 50 and 80 nm) or after 20 phosphate buffer washes ('washed'; 20, 50 and 80 nm), or synthesized by laser ablation with carbon-stabilized surfaces ('carbon-coated'; 25 and 35 nm). Unwashed Ag-nps were toxic to all bacterial strains at concentrations between 3.0-8.0 μg/ml. The washed Ag-nps and carbon-coated Ag-nps were toxic to all bacterial strains except Ag-resistant E. coli at concentrations between 64.0-1024.0 μg/ml. Ag-resistant E. coli died only when treated with unwashed Ag-nps or its supernatant, both of which contained formaldehyde.}, number={2}, journal={NANOTOXICOLOGY}, author={Samberg, Meghan E. and Orndorff, Paul E. and Monteiro-Riviere, Nancy A.}, year={2011}, month={Jun}, pages={244–253} }
 @article{samberg_oldenburg_monteiro-riviere_2010, title={Evaluation of Silver Nanoparticle Toxicity in Skin in Vivo and Keratinocytes in Vitro}, volume={118}, ISSN={["1552-9924"]}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000275449500034&KeyUID=WOS:000275449500034}, DOI={10.1289/ehp.0901398}, abstractNote={IntroductionProducts using the antimicrobial properties of silver nanoparticles (Ag-nps) may be found in health and consumer products that routinely contact skin.ObjectivesThis study was designed to assess the potential cytotoxicity of Ag-nps in human epidermal keratinocytes (HEKs) and their inflammatory and penetrating potential into porcine skin in vivo.Materials and MethodsWe used eight different Ag-nps in this study [unwashed/uncoated (20, 50, and 80 nm particle diameter), washed/uncoated (20, 50, and 80 nm), and carbon-coated (25 and 35 nm)]. Skin was dosed topically for 14 consecutive days. HEK viability was assessed by MTT, alamarBlue (aB), and CellTiter 96 AQueous One (96AQ). Release of the proinflammatory mediators interleukin (IL)-1β, IL-6, IL-8, IL-10, and tumor necrosis factor-α (TNF-α) were measured.ResultsThe effect of the unwashed Ag-nps on HEK viability after a 24-hr exposure indicated a significant dose-dependent decrease (p < 0.05) at 0.34 μg/mL with aB and 96AQ and at 1.7 μg/mL with MTT. However, both the washed Ag-nps and carbon-coated Ag-nps showed no significant decrease in viability at any concentration assessed by any of the three assays. For each of the unwashed Ag-nps, we noted a significant increase (p < 0.05) in IL-1β, IL-6, IL-8, and TNF-α concentrations. We observed localization of all Ag-nps in cytoplasmic vacuoles of HEKs. Macroscopic observations showed no gross irritation in porcine skin, whereas microscopic and ultrastructural observations showed areas of focal inflammation and localization of Ag-nps on the surface and in the upper stratum corneum layers of the skin.ConclusionThis study provides a better understanding Ag-nps safety in vitro as well as in vivo and a basis for occupational and risk assessment. Ag-nps are nontoxic when dosed in washed Ag-nps solutions or carbon coated.}, number={3}, journal={ENVIRONMENTAL HEALTH PERSPECTIVES}, author={Samberg, Meghan E. and Oldenburg, Steven J. and Monteiro-Riviere, Nancy A.}, year={2010}, month={Mar}, pages={407–413} }