@article{wang_ritter_kullman_muddiman_2023, title={Comparative analysis of sucrose-embedding for whole-body zebrafish MSI by IR-MALDESI}, volume={8}, ISSN={["1618-2650"]}, url={https://doi.org/10.1007/s00216-023-04914-1}, DOI={10.1007/s00216-023-04914-1}, journal={ANALYTICAL AND BIOANALYTICAL CHEMISTRY}, author={Wang, Mary F. and Ritter, Morgan M. and Kullman, Seth W. and Muddiman, David C.}, year={2023}, month={Aug} }
 @article{wang_sohn_samal_erning_segura_muddiman_2023, title={Lipidomic Analysis of Mouse Brain to Evaluate the Efficacy and Preservation of Different Tissue Preparatory Techniques by IR-MALDESI-MSI}, volume={3}, ISSN={["1879-1123"]}, DOI={10.1021/jasms.2c00353}, abstractNote={Numerous preparatory methods have been developed to preserve the cellular and structural integrity of various biological tissues for different -omics studies. Herein, two preparatory methods for mass spectrometry imaging (MSI) were evaluated, fresh-frozen and sucrose-embedded, paraformaldehyde (PFA) fixed, in terms of ion abundance, putative lipid identifications, and preservation of analyte spatial distributions. Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)-MSI was utilized to compare the preparatory methods of interest with and without the use of the conventional ice matrix. There were 2.5-fold and 1.6-fold more lipid species putatively identified in positive- and negative-ion modes, respectively, for sucrose-embedded, PFA-fixed tissues without an ice matrix relative to the current IR-MALDESI-MSI gold-standard, fresh-frozen tissue preparation with an exogenous ice matrix. Furthermore, sucrose-embedded tissues demonstrated improved spatial distribution of ions resulting from the cryo-protective property of sucrose and paraformaldehyde fixation. Evidence from these investigations supports sucrose-embedding without ice matrix as an alternative preparatory technique for IR-MALDESI-MSI.}, journal={JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY}, author={Wang, Mary F. and Sohn, Alexandria L. and Samal, Juhi and Erning, Kevin and Segura, Tatiana and Muddiman, David C.}, year={2023}, month={Mar} }