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Works (21)
Updated: April 5th, 2024 07:06
2023 article
Assessing MTT and sulforhodamine B cell proliferation assays under multiple oxygen environments
Yao, M., Walker, G., & Gamcsik, M. P. (2023, July 5). CYTOTECHNOLOGY.
author keywords: MTT assay; SRB; Oxygen partial pressure; Hypoxia; Proliferation; Cancer
TL;DR:
It is found that the MTT readings do not correlate with cell number for the Caco-2 and PANC-1 cell lines under different oxygen conditions, whereas the sulforhodamine B protein assays perform well under all conditions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: July 24, 2023
2022 journal article
Direct Detection of Glutathione Biosynthesis, Conjugation, Depletion and Recovery in Intact Hepatoma Cells
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(9).
author keywords: glutathione; antioxidant; nuclear magnetic resonance spectroscopy; noninvasive; alkylating agent; metabolism
MeSH headings : Animals; Bioreactors; Bridged Bicyclo Compounds; Carcinoma, Hepatocellular; Glutathione / metabolism; Humans; Liver Neoplasms; Rats
TL;DR:
Standard NMR techniques can directly monitor intracellular processes such as glutathione depletion with a time resolution of approximately < 2 min, and are demonstrated to be effective in alginate-encapsulated JM-1 hepatomas.
(via Semantic Scholar)
open access via www.mdpi.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: June 6, 2022
2022 article
Highlighting Functional Mass Spectrometry Imaging Methods in Bioanalysis
Mellinger, A. L., Muddiman, D. C., & Gamcsik, M. P. (2022, June 27). JOURNAL OF PROTEOME RESEARCH, Vol. 6.
author keywords: mass spectrometry imaging; kinetics; rate; isotope; metabolism; dynamics
MeSH headings : Magnetic Resonance Imaging; Mass Spectrometry / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
MSI exceeds some of the classic functional imaging methods, demonstrating the ability to provide functional data from the nanoscale (subcellular) to whole tissue or organ level, and this Perspective highlights several examples of how different MSI ionization and detection technologies can provide unprecedented detailed spatial maps of time-dependent biological processes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 11, 2022
2022 journal article
Mapping glycine uptake and its metabolic conversion to glutathione in mouse mammary tumors using functional mass spectrometry imaging
FREE RADICAL BIOLOGY AND MEDICINE, 193, 677–684.
MeSH headings : Mice; Animals; Glycine; Biological Transport; Glutathione; Mammary Neoplasms, Animal; Mass Spectrometry
TL;DR:
A novel functional mass spectrometry imaging (fMSI) method that can map the variations in the conversion of glycine to glutathione metabolic activity across tumor tissue sections by tracking the fate of three glycine isotopologues administered in a timed sequence to tumor-bearing anesthetized mice is developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 23, 2023
2021 journal article
A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments
SCIENTIFIC REPORTS, 11(1).
MeSH headings : Cell Culture Techniques / instrumentation; Cell Culture Techniques / methods; Cell Hypoxia / drug effects; Cell Hypoxia / physiology; Cell Line, Tumor / metabolism; Doxorubicin / pharmacology; High-Throughput Screening Assays / methods; Humans; Neoplasms / metabolism; Oxygen / metabolism; Pancreatic Neoplasms / metabolism; Tirapazamine / pharmacology; Toxicity Tests / methods; Triazines / pharmacology; Tumor Microenvironment / physiology
TL;DR:
A novel 96-well plate design is presented that recirculates the local oxygen environment to shield cells during media changes and facilitates toxicity studies of cells cultured under varying oxygen levels, ideal for assessing the effects of oxygen as a variable in toxicity screens.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: March 22, 2021
2021 journal article
Multiple Infusion Start Time Mass Spectrometry Imaging of Dynamic SIL-Glutathione Biosynthesis Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization
JOURNAL OF PROTEOME RESEARCH, 21(3), 747–757.
author keywords: IR-MALDESI; mass spectrometry imaging; stable isotope labeling; glutathione; kinetic metabolism
MeSH headings : Animals; Glutathione; Glycine; Lasers; Mice; Spectrometry, Mass, Electrospray Ionization / methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
TL;DR:
A study combining a multiple infusion start time protocol, stable isotope labeling technology, infrared matrix-assisted laser desorption electrospray ionization, and high-resolution accurate mass-mass spectrometry imaging to study spatial changes in glutathione kinetics across in sectioned mouse liver tissues is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 2, 2022
2019 journal article
Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia
ANALYTICAL CHEMISTRY, 91(23), 15032–15039.
MeSH headings : Aldehyde Dehydrogenase / metabolism; Caco-2 Cells; Cell Hypoxia; Cell Line, Tumor; Glucose / pharmacokinetics; Humans; Hypoxia-Inducible Factor 1, alpha Subunit / drug effects; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; Oxygen / metabolism; Oxygen / pharmacology; Pancreatic Neoplasms / pathology; Time Factors
TL;DR:
A manifold designed to use flow-encoding to produce sequential changes in gas mixtures delivered to a permeable-bottom 96-well plate is fabricated and shown how this manifold and plate design can be used to expose cells to either static or cycling hypoxic conditions for eight different time periods thereby facilitating the study of the time-response of cells to altered oxygen environments.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 30, 2019
2018 journal article
Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 315(5), C766–C775.
author keywords: cell culture; cycling hypoxia; finite element analysis; oxygen diffusion; permeability
MeSH headings : Caco-2 Cells; Cell Culture Techniques / instrumentation; Cell Hypoxia / genetics; Cell Proliferation / genetics; Humans; Hypoxia-Inducible Factor 1, alpha Subunit / chemistry; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; MCF-7 Cells; Oxygen / chemistry; Oxygen / metabolism
TL;DR:
A 96-well plate-based device that is capable of delivering eight static or dynamically changing oxygen environments to different rows on a single plate that is amenable to multiwell Plate-based molecular assays or drug dose-response studies in static or cycling hypoxia conditions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 10, 2018
2015 journal article
Biocompatibility of Tygon® tubing in microfluidic cell culture
Biomedical Microdevices, 17(1).
author keywords: Biomaterial; Cancer cell; Biocompatibility; Toxicity; Polyvinylchloride; Spectroscopy
MeSH headings : Cell Culture Techniques / instrumentation; Cell Culture Techniques / methods; Cell Line, Tumor; Female; Humans; Microfluidic Analytical Techniques / instrumentation; Microfluidic Analytical Techniques / methods
TL;DR:
Results indicate leaching of a chemical species from microbore Tygon® tubing that can affect cell growth in microfluidic devices.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: August 28, 2020
2014 journal article
A microfluidic device to study cancer metastasis under chronic and intermittent hypoxia
Biomicrofluidics, 8(5), 054117.
TL;DR:
This work presents the design and characterization of a microfluidic device that can simultaneously mimic the oxygenation conditions observed within the tumor and model the cell migration and intravasation processes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Noninvasive In Vivo Magnetic Resonance Measures of Glutathione Synthesis in Human and Rat Liver as an Oxidative Stress Biomarker
HEPATOLOGY, 59(6), 2321–2330.
MeSH headings : Adult; Animals; Biomarkers / metabolism; Carbon Isotopes; Glutathione / biosynthesis; Glycine; Humans; Liver / metabolism; Magnetic Resonance Imaging; Male; Oxidative Stress; Rats; Rats, Sprague-Dawley; Translational Research, Biomedical
TL;DR:
In vivo 13C‐labeling and detection of GSH as a biomarker of tissue OS defenses, detecting chronic and acute OS insults are demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
13C magnetic resonance spectroscopy detection of changes in serine isotopomers reflects changes in mitochondrial redox status
MAGNETIC RESONANCE IN MEDICINE, 68(3), 671–679.
author keywords: serine 13C isotopomers; 13C NMR spectroscopy; mitochondrial redox; 2-13C-glycine; hepatocytes; confocal microscopy
MeSH headings : Animals; Carbon Isotopes; Cells, Cultured; Hepatocytes / metabolism; Hepatocytes / ultrastructure; Male; Mitochondria, Liver / metabolism; NAD / analysis; Oxidation-Reduction; Radiopharmaceuticals; Rats; Rats, Sprague-Dawley; Serine / analysis
TL;DR:
It is demonstrated that 13C magnetic resonance spectroscopy monitoring of the formation of serine isotopomers in isolated rat hepatocytes given 2‐13C‐glycine reflects the changes of mitochondrial redox status.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
In vivo MR studies of glycine and glutathione metabolism in a rat mammary tumor
NMR IN BIOMEDICINE, 25(2), 271–278.
author keywords: glutathione; MR; serine; metabolism; trans-sulfuration
MeSH headings : Animals; Carbon Isotopes; Female; Glutathione / metabolism; Glycine / metabolism; Magnetic Resonance Spectroscopy / methods; Mammary Neoplasms, Animal / metabolism; Mammary Neoplasms, Animal / pathology; Mass Spectrometry; Neoplasm Transplantation; Rats; Rats, Inbred F344; Subcutaneous Tissue / pathology
TL;DR:
These studies demonstrate the use of MRI and MRS for the monitoring of tumor metabolic processes central to oxidative stress defense and show that isotope‐labeled serine is further metabolized via the trans‐sulfuration pathway.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
In vivo hypoxia and a fungal alcohol dehydrogenase influence the pathogenesis of invasive pulmonary aspergillosis
PLoS Pathogens, 7(7).
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
Synthesis of 13C-labeled derivatives of cysteine for magnetic resonance imaging studies of drug uptake and conversion to glutathione in rat brain
Journal of Labelled Compounds and Radiopharmaceuticals, 54(9), 607–612.
author keywords: C-13-labeling; synthesis; N-acetylcysteine; 2-oxothiazolidine-4-carboxylic acid; OTZ; ethyl glutamylcysteinate
TL;DR:
Three prodrugs of glutathione and cysteine were synthesized with a 13C-label such that in vivo uptake of each and conversion to glutathion in the brain could be monitored by magnetic resonance imaging.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: August 6, 2018
2010 journal article
Non-invasive Monitoring of L-2-Oxothiazolidine-4-Carboxylate Metabolism in the Rat Brain by In vivo 13C Magnetic Resonance Spectroscopy
Neurochemical Research, 36(3), 443–451.
author keywords: Magnetic resonance; Metabolism; Cysteine; Glutathione; Taurine
MeSH headings : Animals; Brain / metabolism; Female; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular / methods; Oxidative Stress; Pyrrolidonecarboxylic Acid / metabolism; Rats; Rats, Inbred F344; Thiazolidines / metabolism
TL;DR:
These studies demonstrate the utility of in vivo magnetic resonance for non-invasive monitoring of antioxidant uptake and metabolism in intact brain and evaluate the efficacy of various interventions for maintenance of brain glutathione.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID, NC State University Libraries
Added: August 27, 2020
2005 journal article
Intrastrand base-stacking buttresses widening of major groove in interstrand cross-linked B-DNA
Bioorganic & Medicinal Chemistry, 13(14), 4580–4587.
author keywords: DNA interstrand crosslinks; DNA repair; solution NMR structure
MeSH headings : DNA / chemistry; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Conformation
TL;DR:
Compared to earlier structures of ethyl cross-linked oligonucleotides, this unique perturbation induced by the butyl moiety offers a new probe for systematic studies of DNA repair mechanisms.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2005 journal article
Structure, Flexibility, and Repair of Two Different Orientations of the Same Alkyl Interstrand DNA Cross-Link†
Biochemistry, 44(18), 6764–6775.
MeSH headings : Alkylating Agents / chemistry; CpG Islands; Cross-Linking Reagents / chemistry; DNA Repair; DNA, Circular / chemistry; Intercalating Agents / chemistry; Microscopy, Atomic Force; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Conformation; Nucleic Acid Heteroduplexes / chemical synthesis; Plasmids; Thermodynamics
TL;DR:
It is shown that the increased flexibility observed in the GpC cross-link persists when it is embedded into much larger DNA fragments, which may account for the different repair efficiencies seen in NER deficient cells.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2004 journal article
A versatile oxygenator and perfusion system for magnetic resonance studies
Biotechnology and Bioengineering, 49(3), 348–354.
author keywords: oxygenator; NMR spectroscopy; organ perfusion; mammalian cell culture
TL;DR:
A compact, reusable membrane oxygenator has been constructed for the perfusion of cultured cells and isolated organs that is compatible with nuclear magnetic resonance (NMR) spectroscopy studies and can be used for any experiment which requires warming and oxygenation of perfusates.
(via Semantic Scholar)
Source: Crossref
Added: January 28, 2021
2004 journal article
Accommodation of Mispair Aligned N3T-Ethyl-N3T DNA Interstrand Cross Link†
Biochemistry, 43(39), 12549–12554.
MeSH headings : Base Pair Mismatch; Base Sequence; Cross-Linking Reagents / chemistry; Crystallography, X-Ray; DNA / chemical synthesis; DNA / genetics; DNA Damage; DNA Repair; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Heteroduplexes / chemistry; Nucleic Acid Heteroduplexes / genetics; Oligonucleotides / chemistry; Oligonucleotides / genetics; Thermodynamics; Thymine / chemistry
TL;DR:
It is established that the lesion defined by mismatch-aligned minor groove N(3)T-ethyl-N( 3)T cross-linked thymine bases produces very localized distortions in a DNA stem that may be difficult to recognize by repair mechanisms that are not transcription- or replication-coupled, and is a valuable structural probe to study mechanisms of repair.
(via Semantic Scholar)
Source: Crossref
Added: January 21, 2021
2004 journal article
Role of glutathione and nucleotide excision repair in modulation of cisplatin activity with O6-benzylguanine
Cancer Chemotherapy and Pharmacology, 55(4), 333–342.
author keywords: cisplatin; modulation; chemotherapy
MeSH headings : Antineoplastic Agents / pharmacology; Cell Line; Cell Survival / drug effects; Cisplatin / pharmacology; DNA Damage; DNA Repair / drug effects; Drug Synergism; Glutathione / metabolism; Guanine / analogs & derivatives; Guanine / pharmacology; Humans; Platinum / metabolism; Tumor Cells, Cultured
TL;DR:
The results suggest that the mechanism of enhancement involves neither the GSH nor the NER pathways, but triggers an event prior to DNA platination damage that ultimately results in increased cytotoxicity, apoptosis and increased platination levels.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: January 21, 2021
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