@article{rogers_poore_shaeffer_weaver_siciliano_harmon_2024, title={Amazing Grazing webinar and workshop series improves producers understanding of pasture ecology}, volume={102}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skae019.010}, abstractNote={Abstract
               Grassland stewards are seeking a better understanding of the complex interactions occurring in their pastures. Information is readily available to assist producers but each farm provides its own unique challenges and opportunities. A diverse scope of knowledge and skills are required to build resilient grazing systems. To address this need Amazing Grazing conducted a webinar series (April 2023) followed by on-farm workshops in July/August 2023. Webinars were held on consecutive Wednesday evenings with 237 average number of registered attendees and 78 average number of actual participants. Topics covered during the first webinar were pasture ecology basics, plant growth principles, livestock forage demand and grazing management principles. The following week covered pasture layout and design, pasture renovation techniques and pasture soil health concepts. The final evening featured topics on electric fence basics, grazing systems for horses and small ruminants and a synopsis. NC Extension Livestock Agents assisted in the sight selection and execution of workshops (n = 8) that were held on working farms. These events provide the opportunity for hands-on instruction to demonstrate the concepts covered in the webinar series. Total workshop registration was 196, with 148 evaluations completed. Participants were 72% farmers, 1% extension agents, 10% Soil and Water Conservation District Employee, 7% NRCS employee and 10% other. The total number of pasture hectares grazed by participants was 3,217 with 1,257 ha as hay. Livestock inventories for attendees were 4,323 beef cattle, 1,366 sheep, 455 goats and 51 horses. Attendees were asked about their pre- and post- workshop knowledge (1 = Very Low; 5 = Very High) of topics presented. Participants indicated a knowledge gain (average pre = 2.78 and post = 3.91) in Concepts of Pastureland Ecology, Adaptive Management, Managing Nutrient Distribution, Pasture Renovation, Temporary Electric Fence, Troubleshooting Electric Fence and Pasture Plant Identification. Workshop impact was determined by asking attendees their post workshop intentions regarding management practices (1 = No, 2 = Maybe, 3 = Yes, 4 = Already Doing). Attendees plan to spending more time walking pasture to determine plant condition, learn more about soil, plant, animal and human interactions, increase use of temporary electric fence and adopt an adaptive management style (average score = 3.91). Continued workshop participation indicates the demand for hands-on education in these areas and that the on-farm format is an effective teaching environment.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Rogers, Johnny R. and Poore, Matt H. and Shaeffer, April D. and Weaver, Andrew R. and Siciliano, Paul and Harmon, Deidre D.}, year={2024}, month={Mar}, pages={7–8} }
 @article{huggins_poore_harmon_2024, title={Cotton gin byproduct in growing diets for beef steers}, volume={102}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skae234.569}, abstractNote={Abstract The purpose of this study was to evaluate the performance of beef cattle during the stocker phase when fed different concentrations of cotton gin byproduct (CGB). Angus steers [n = 50; initial body weight (BW) = 349 ± 66 kg) were stratified by BW and grouped in slatted floor pens (10 steers/pen). Steers were assigned randomly within pen (2 per pen) to one of five diets containing 0, 10, 20, 30, and 40% CGB (dry matter basis). Diets were balanced for protein and energy, and the steers were fed for 84 d. Steers were weighed on d -1, 0, 28, 56, 83, and 84 for the stocker phase with d -1 and 0, 83 and 84 averaged to give start and end BW. Following the growing period, steers were switched to the same finishing diet and fed for an additional 55 d prior to being transported to PA for harvest and carcass data collection. Data were analyzed using procMIXED of SASv9.4 to determine the significance of linear, quadratic, cubic, and quartic trends. During the growing phase, dry matter intake increased linearly (P = 0.0017) with 10.16, 11.23, 11.59, 11.86, and 11.96 kg/d for 0, 10, 20, 30, and 40% CGB, respectively. Average daily gain tended to increase (P = 0.0631) with a cubic trend, increasing with the first addition of CGB, declining at the intermediate levels, and then increasing again at the 40% level (1.34, 1.54, 1.36, 1.39, and 1.52 kg/d for 0, 10, 20, 30, and 40% CGB, respectively). Feed efficiency showed a similar cubic trend (P = 0.10) with 0.133, 0.138, 0.119, 0.117, and 0.128 for 0, 10, 20, 30, and 40% CGB, respectively. Within the finishing phase, average daily gain was significant (P = 0.026) as a quadratic trend with 1.54, 1.25, 1.18, 1.20, and 1.33 kg/d for 0, 10, 20, 30, and 40% CGB, respectively. Diets did not differ (P > 0.10) for dry matter intake (10.08 kg/d), feed conversion (8.31), or feed efficiency (0.130). Treatment did not influence (P > 0.10) marbling or other carcass measurements. Data suggest CGB supported good animal gains with only minor impact on feed intake and feed efficiency for stocker steers up to an inclusion rate of 40%.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Huggins, Michael J. and Poore, Matt H. and Harmon, Deidre}, year={2024}, month={Sep}, pages={503–503} }
 @article{poore_shaeffer_rogers_weaver_siciliano_harmon_2024, title={Pastureland Ecology 1 provides hands-on training in grazing management for USDA-NRCS professionals}, volume={102}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skae019.007}, abstractNote={Abstract
               Pastureland Ecology 1 is a 2-wk continuing education course taught by cooperating faculty at NC State University for USDA-NRCS Staff. The course was founded in the early 1990s and has been taught 23 times to over 500 participants from all 50 states. Participants primarily include grazing specialists, soil conservationists, and district conservationists. Instruction is split between classroom lectures and hands-on experience with grazing management. Lecture topics include; introduction to pasture ecology, principles of plant growth, determining animal forage needs, pasture design, principles of soil health, principles of grazing management, and grazing management systems for small ruminants, horses and cattle. Small groups of students work with groups of goats, sheep, horses, and beef cattle throughout the course, learning concepts of forage allocation, temporary electric fence, and adaptive management. Popular demonstrations developed over the years include; the reel race, dung beetle buckets, burying underwear to demonstrate soil health, exploring root diversity, and troubleshooting electric fence. Farm tours are conducted to show how concepts being taught are applied on commercial farms. In 2023 there were 29 students from 13 states including VA (4), MD (4), PA (3), OK (4), MI (1), UT (2), TN (3), WV (1), MA (1), OH (1), NM (1), WA (3), and RI (1). Participants had 8.4 ± 1.39 (AVG ± SEM) years of experience with NRCS with a range of 1 to 27 yr. Students self-assessed their understanding of major concepts before and after the course. On a 1 to 5 scale students increased their understanding by an average of 1.05 ± 0.129, averaged over all topics. Topics more familiar to participants had less increase including; Soil Health (0.38), Pasture Condition Evaluation (0.51), and Grazing Management for Beef Cattle (0.79). Less familiar topics had greater increases including Grazing Management for Horses (1.27) and Small Ruminants (1.27), and Temporary Electric Fence (1.43). Pastureland Ecology 1 remains a critical part of the Amazing Grazing Program at NC State University, impacting pasture-based livestock producers across the country. This educational model has proven to be an effective way of teaching the principles of pastureland ecology.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poore, Matt H. and Shaeffer, April D. and Rogers, Johnny R. and Weaver, Andrew R. and Siciliano, Paul and Harmon, Deidre}, year={2024}, month={Mar}, pages={5–5} }
 @article{mullenix_gadberry_rhinehart_dillard_tucker_stewart_vendramini_poole_sanchez_mercadante_et al._2023, title={Awardee Talk: Foraging for Collaboration: Beef-Forage System Strengths Unite Research and Extension Efforts in the Asas Southern Section}, volume={101}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skad281.187}, abstractNote={Abstract
               The American Society of Animal Science Southern Section has a rich history of collaboration and hosts an annual professional development meeting for ASAS members. The Section meeting includes scientific presentations, academic quadrathlon, undergraduate and graduate student competitions, symposia, and networking opportunities for its members. The Section has used strategic collaborations to foster multi-state projects and initiatives. One of the key discipline focus areas of the Section is forage-based beef production systems. The Section holds two symposia focused on research, Extension, and industry topics related to beef cattle management. The Bill E. Kunkle Interdisciplinary Symposium was started in 2014 to honor the impact of Dr. Kunkle, University of Florida, and fosters multi-disciplinary communication of beef cattle science. The SERA 41 Symposium is part of the regional NIMSS project focus-group on improving production efficiency of the beef cow herd in the Southeast US. The NIMSS group works collectively to create partnerships and common goal-oriented projects across the Southern Section states. The section is also home to the Randel Physiology Lecture series which was formed in 2016 to highlight Dr. Ron Randel’s commitment to the Southern Section physiology section, and to promote high-quality science for the next generation of physiologists and endocrinologists. More than 25 peer-reviewed publications and several webinars have resulted from these symposia and invited lectureships. This creates a referenceable resource for scientists on emerging topics and identifies potential priority areas of focus for scientists within the Southern region. Another highlight of the Section is the collaborative work of the Extension committee. The Extension committee collaboratively fosters Extension program development across the Southern region. The committee developed a regional webinar series in 2015 for regional training of Extension agents on livestock and forage management topics. Subsequent in-service training courses have emerged through both virtual and in-person delivery. Training is primarily focused on forage-based livestock management systems. Through these opportunities, the Southern Section has remained a gathering point for professional engagement and should continue to evolve to meet the needs of animal scientists.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Mullenix, Kimberly and Gadberry, Shane and Rhinehart, Justin D. and Dillard, S. Leanne and Tucker, Jennifer J. J. and Stewart, Robert Lawton and Vendramini, Joao and Poole, Daniel H. and Sanchez, Nicole C. Burdick and Mercadante, Vitor R. G. and et al.}, year={2023}, month={Nov}, pages={152–153} }
 @article{poore_2023, title={Challenges in the Pasture}, volume={101}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skad068.116}, abstractNote={Abstract
               Cow-calf farms are abundant across the southeastern US and there is a wide diversity of farm sizes and types of farmers. Farms include small-scale part-time farms, and large commercial-scale farms, often integrated with other farm enterprises. Many farmers have full-time employment off the farm and little if any hired labor, such that there is little time to devote to farming activities. In contrast, large farms often have access to labor, equipment and other inputs that may not be available to small farms. The diversity of farms and farmer demographics makes extension programming challenging as motivational factors vary considerably. Many pasture management practices proven by research have very low levels of adoption including active grazing management, pasture renovation, hay testing, etc. We assume that all farmers are focused on profitability, but this is clearly not the case especially with small part-time farmers. Other motivations may be enjoyment of spending time with animals, time spent with family, improving animal welfare, interest in machinery and “gadgets”, and other non-farm interests including using a cow-calf enterprise to hold land in some kind of agriculture for future development. New management practices may make sense from a profitability perspective but might require the development of new skills that the farmer does not have. Additionally, there may be other barriers including access to necessary equipment, conflicting information provided by advisers, and expertise in simple practices such as planting and spraying. As an example, novel endophyte tall fescue (NETF) has potential to eliminate the negative aspects of endophyte infected tall fescue while maintaining the desirable agronomic characteristics. While NETF technology is research proven and has been available to producers for over two decades, adoption has been very slow. The Alliance for Grassland Renewal is a multi-state private/public collaborative formed to promote the appropriate adoption of NETF. The Alliance conducts workshops to teach farmers and their advisors about how to best implement the NETF technology. The collaborative effort provides a more uniform set of recommendations than was formerly available through each member state. Through workshop evaluations we found several important factors that limit adoption including 1) the balance of cost and benefit is unclear, 2) farmers have limited financial resources, 3) the land is rented or unsuitable for renovation, and 4) farmers lack of knowledge, and 5) farm forage reserves are limiting. Many of these pasture management practices are difficult to adopt because they require many sequential steps that must be completed in a timely way. This may leave the farmer hesitant to take the first step in the process. For practices that are very different than standard management, use of on-farm demonstrations and hands-on learning have proven valuable.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poore, Matthew H.}, year={2023}, month={May} }
 @article{newsome_poore_harmon_shaeffer_talbott_fellner_griffith_2023, title={The Effects of Varying Levels of Cotton Gin Byproduct and Wet brewers' Grains on Growth Performance of Growing and Finishing Steers}, volume={101}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skad281.345}, abstractNote={Abstract
               The abundance of craft breweries provides widespread availability of wet brewers grains (WBG) to be used by beef cattle producers as a potential feedstuff. Cotton gin byproduct (CGB) is a feedstuff typically reserved for mature beef cows due to its low and variable feed value. Both WBG and CGB have high cost of transportation but are sometimes used by producers near supply centers due to their low price. The objective of this study was to analyze animal performance of growing Angus steers fed total mixed rations with varying amounts of CGB and WBG. Sixty-four Angus steers (365 ± 26 kg) were allotted and blocked by initial body weight (BW) in a randomized complete block design to 8 dietary treatments and fed in 2 phases, growing phase (GP) and finishing phase (FP) (84-d and 69-d, respectively). Dietary treatments were arranged in a 2 x 4 factorial with two levels of CGB (0 or 25% of DM) and 4 levels of WBG (0, 10, 20 or 30% of DM). The control and base of all treatments were corn, corn silage and soybean meal. Body weights were recorded on two consecutive days at the beginning and end of GP and FP. Feed samples were collected, and refusals were weighed weekly. Data were analyzed using the MIXED procedure of SAS 9.4 with main effects of CGB, WBG, and the interaction. The linear, quadratic, and cubic effects of increasing levels of WBG and CGB were tested with polynomial contrasts. Average daily gain (ADG) did not differ among dietary treatments (0, 25% CGB and 0, 10, 20, 30% WBG) for GP, (1.67, 1.61, and 1.67, 1.57, 1.74, and 1.60 kg/d, respectively) (P > 0.3031), FP (1.53, 1.61 and 1.56, 1.53, 1.61, and 1.58 kg/d) or the overall trial (1.61, 1.62 and 1.62, 1.55, 1.68, and 1.60 kg/d). The addition of CGB increased GP dry matter intake (DMI; 11.06 vs 11.99 kg/d; P = 0.0011), while the addition of WBG linearly decreased GP DMI (11.81, 11.69. 11.81, and 10.81 kg/d; P = 0.0292). There was an interaction between CGB and WBG (P = 0.0206), with increasing levels of WBG at 0% CGB exhibiting a cubic effect (P = 0.0127), but no effect at 25% CGB. Inclusion of CGB decreased feed efficiency (FE) in the GP (6.77 vs. 7.69; P = 0.0008) but had no carry-over effect in the FP. There was no effect of WBG on FE in GP or FP. In conclusion, CGB increased DMI, reduced FE, and maintained ADG. Increasing quantities of WBG did not reduce ADG or FE, but decreased DMI with increasing feeding level. Therefore, WBG and CGB can be considered viable options for cattle producers seeking to lower input costs.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Newsome, Madeline and Poore, Matt H. and Harmon, Deidre D. and Shaeffer, April D. and Talbott, Charlotte L. and Fellner, Vivek and Griffith, Emily H.}, year={2023}, month={Nov}, pages={289–290} }
 @article{franzluebbers_shoemaker_cline_lipscomb_stafford_farmaha_waring_lowder_thomason_poore_2022, title={Adjusting the N fertilizer factor based on soil health as indicated by soil-test biological activity}, volume={7}, ISSN={["2471-9625"]}, DOI={10.1002/ael2.20091}, abstractNote={AbstractAgriculture faces a dilemma with nitrogen (N)—it is often the most necessary external input to optimize production, several generations of farmers became accustomed to its relatively inexpensive cost, and it contributes to widespread pollution due to numerous loss pathways to the environment. However, standard N fertilizer recommendations have not accounted well enough for a key source via mineralizable soil N. Soil‐test biological activity (STBA) is strongly associated with mineralizable soil N, both of which become surface‐enriched with conservation agricultural management using soil health principles. A series of field experiments assessed the contribution of mineralizable soil N to the N supply needed to optimize corn (Zea mays L.) grain and fall‐stockpiled tall fescue [Schedonorus arundinaceus (Schreb.) Dumort.] production. This essay synthesizes how STBA along with cost‐to‐value threshold can be used to modify the N fertilizer factor to optimize economic return and avoid environmental degradation.}, number={2}, journal={AGRICULTURAL & ENVIRONMENTAL LETTERS}, author={Franzluebbers, Alan J. and Shoemaker, Robert and Cline, Jeff and Lipscomb, Bruce and Stafford, Carl and Farmaha, Bhupinder S. and Waring, Robert and Lowder, Nathan and Thomason, Wade E. and Poore, Matt H.}, year={2022} }
 @article{palmer_vedovatto_oliveira_ranches_vendramini_poore_martins_binelli_arthington_moriel_2022, title={Effects of maternal winter vs. year-round supplementation of protein and energy on postnatal growth, immune function, and carcass characteristics of Bos indicus-influenced beef offspring}, volume={100}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skac003}, abstractNote={AbstractThis 2-yr study evaluated the effects of winter vs. year-round supplementation of Bos indicus-influenced beef cows on cow reproductive performance and impact on their offspring. On day 0 of each year (approximately day 122 ± 23 of gestation), 82 to 84 mature Brangus cows/yr were stratified by body weight (BW; 475 ± 67 kg) and body condition score (BCS; 4.85 ± 0.73) and randomly assigned to 1 of 6 bahiagrass (Paspalum notatum) pastures (13 to 14 cows/pasture). Treatments were randomly assigned to pastures consisting of winter supplementation with molasses + urea (WMOL), or year-round supplementation with molasses + urea (YMOL) or wheat middling-based range cubes (YCUB). Total yearly supplement DM amount was 272 kg/cow and supplements were formulated to be isocaloric and isonitrogenous (75% TDN and 20% CP). On day 421 (weaning; approximately 260 ± 24 d of age), 33 to 35 steers/yr were vaccinated against parainfluenza-3 (PI3) and bovine viral diarrhea virus type 1 (BVDV-1) and transported 1,193 km to a feedlot. Steers were penned according to maternal pasture and managed similarly until slaughter. Data were analyzed using the MIXED and GLIMMIX procedures of SAS. On day 217 (start of breeding season), BCS was greater (P = 0.01) for YMOL than WMOL cows, whereas BCS of YCUB did not differ (P ≥ 0.11) to both WMOL and YMOL cows. The percentage of cows that calved, calving date, birth BW, and preweaning BW of the first offspring did not differ (P ≥ 0.22) among maternal treatments. Plasma cortisol concentrations were greater (P ≤ 0.001) for YCUB steers at feedlot arrival (day 422) than WMOL and YMOL steers. Moreover, YCUB steers had greater (P = 0.02) and tended (P = 0.08) to have greater plasma concentrations of haptoglobin compared to WMOL and YMOL steers, respectively. Antibody titers against PI3 and BVDV-1 viruses did not differ (P ≥ 0.25) among maternal treatments. Steer BW at feedlot exit was greater (P ≤ 0.05) for YMOL and WMOL than YCUB steers. However, feedlot DMI did not differ (P ≥ 0.37) by maternal treatment. Hot carcass weight, yield grade, LMA, and marbling did not differ (P ≥ 0.14) among maternal treatments. Percentage of steers that graded low choice was enhanced (P ≤ 0.05) for WMOL and YCUB than YMOL steers. Maternal year-round supplementation of range cubes or molasses + urea either did not impact or decrease growth, immune function, and carcass characteristics of the offspring when compared with maternal supplementation of molasses + urea during winter only.}, number={3}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Palmer, Elizabeth A. and Vedovatto, Marcelo and Oliveira, Rhaiza A. and Ranches, Juliana and Vendramini, Joao M. B. and Poore, Matthew H. and Martins, Thiago and Binelli, Mario and Arthington, John D. and Moriel, Philipe}, year={2022}, month={Mar} }
 @misc{poore_2022, title={Invited Review: Use of byproduct feeds in southeastern US beef production systems}, volume={38}, ISSN={["2590-2865"]}, DOI={10.15232/aas.2022-02307}, abstractNote={Byproduct feeds are important in beef cattle feeding systems in the southeastern United States. This article discusses the evolution of a feed from a waste material to a coproduct, presents a current understanding of several important byproducts, and details sweetpotatoes and associated byproducts as an example of a potential feed source that needs continued development. Applied research and outreach results are presented to support the use of byproducts currently in widespread use in the region. Practical advice is offered to readers based on 32 yr of experience working with farmers on byproduct-based diets for stocker cattle and brood cows. There are several well-established byproduct feeds, including soybean hulls, corn gluten feed, distillers grains, and wheat middlings, that have been the subject of a great deal of research and development. Many other potential feed sources remain as waste materials or waste products awaiting more research and development to help them evolve into well-accepted feeds. Nutritionists need to understand the characteristics and limitations of byproducts available in their area. Working with producers to set up effective feeding programs that allow them to make quick decisions on ingredient purchasing and adapting to disruption in supply will be critical. Although many feeds appear valuable "on paper" using the Petersen method, factors that influence their usefulness, including the presence of contaminants or antinutritional factors, high moisture content, and imbalance of key minerals, must be considered in the development of an effective feeding program.}, number={5}, journal={APPLIED ANIMAL SCIENCE}, author={Poore, Matthew H.}, year={2022}, month={Oct}, pages={441–446} }
 @article{franzluebbers_poore_2022, title={Soil fertility characteristics in North Carolina pastures as affected by spatial separation and renovation with annual forages}, ISSN={["1435-0645"]}, DOI={10.1002/agj2.21218}, abstractNote={AbstractSpatial variation in soil properties is often considered significant across broad geographical regions due to soil formation factors. However, fine‐scale variations might also be significant. This study was conducted with the original intent of assessing how simple and complex mixtures of annual forages might be used to renovate perennial pastures. Private farmers in the Flatwoods, Piedmont, and Blue Ridge Major Land Resource Areas of North Carolina tested annual forages to renovate tall fescue pastures. Soil was sampled in multiple random locations in each field at depths of 0–6, 6–12, and 12–20 cm at the beginning and ending of a 3‐yr annual forage evaluation. Relative variation among five components (year of sampling [n = 2], physiographic region [n = 3], annual forage treatment [n = 2], soil depth [n = 3], and random variation from pseudoreplicates [n = 3 in 2015 and n = 5 in 2018]) was assessed for four soil physical, 10 soil biological, and 16 soil chemical properties. Soil chemical properties were mostly affected by physiographic region (47 ± 26% of total variation) and soil depth (33 ± 18%), soil biological properties were mostly affected by soil depth (63 ± 25%) and random pseudoreplication (14 ± 6%), and soil physical properties were equally affected by pseudoreplication (35 ± 21%), physiographic region (32 ± 18%), and soil depth (29 ± 22%). The type of annual forage had no discernible effect on soil properties, even the most biologically active. A diversity of spatial variations was important, suggesting that regional‐level ecological investigations require careful attention to an appropriate sampling design considering multiple factors.}, journal={AGRONOMY JOURNAL}, author={Franzluebbers, Alan. J. and Poore, Matt H.}, year={2022}, month={Dec} }
 @article{palmer_vedovatto_oliveira_ranches_vendramini_poore_martins_binelli_arthington_moriel_2022, title={Timing of maternal supplementation of dried distillers grains during late gestation influences postnatal growth, immunocompetence, and carcass characteristics of Bos indicus-influenced beef calves}, volume={100}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skac022}, abstractNote={AbstractThis 2-yr study investigated the timing of dried distillers grains (DDG) supplementation during the third trimester of gestation of Bos indicus-influenced beef cows and its impact on their offspring performance. On day 0 of each year (84 d before calving), Brangus cows (n = 84/yr; cow age = 8 ± 3 yr) were stratified by initial body weight (BW; 482 ± 75 kg) and body condition score (BCS; 5.3 ± 0.8) and assigned randomly to one of six bahiagrass (Paspalum notatum) pastures (experimental units; 14 cows/pasture). Treatments were assigned randomly to pasture (2 pastures/treatment/yr) and consisted of no prepartum supplementation (CON), 2 kg/d of DDG from day 0 to 42 (LATE42), or 1 kg/d of DDG from day 0 to 84 (LATE84). Following calving (day 84), cow–calf pairs remained in their respective pastures, and cows were offered sugarcane molasses + urea (1.82 kg of dry matter/cow/d) from day 85 until the end of the breeding season (day 224). On day 347, steer calves (n = 38/yr; 11 to 15 steers/treatment/yr) were weaned and transported to the feedlot (1,193 km). Steers were penned according to cow prepartum pasture and managed similarly until the time of harvest. BCS at calving was greater (P < 0.01) for LATE42 and LATE84 vs. CON cows but did not differ (P = 0.16) between LATE42 and LATE84 cows. Calving date, calving percentage, and birth BW of the first offspring did not differ (P ≥ 0.22) among treatments. However, LATE42 cows calved their second offspring 8 d earlier (P = 0.04) compared with CON and LATE84 cows. At weaning (first offspring), LATE84 calves were the heaviest (P ≤ 0.05), CON calves were the lightest, and LATE42 calves had intermediate BW (P ≤ 0.05). Steer plasma concentrations of cortisol and haptoglobin and serum bovine viral diarrhea virus type-1 titers did not differ (P ≥ 0.21) between treatments. Steer serum parainfluenza-3 titers were greater (P = 0.03) for LATE42 vs. CON steers, tended to be greater (P = 0.10) for LATE84 compared with CON steers, and did not differ (P = 0.38) between LATE42 and LATE84 steers. Steer feedlot BW, average daily gain, dry matter intake, and hot carcass weight did not differ (P ≥ 0.36) between treatments. Marbling and the percentage of steers grading choice were greater (P ≤ 0.04) for LATE42 vs. CON steers, whereas LATE84 steers were intermediate. In summary, different timing of DDG supplementation during the third trimester of gestation could be explored to optimize cow BCS and offspring preweaning growth and carcass quality.}, number={2}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Palmer, Elizabeth A. and Vedovatto, Marcelo and Oliveira, Rhaiza A. and Ranches, Juliana and Vendramini, Joao M. B. and Poore, Matthew H. and Martins, Thiago and Binelli, Mario and Arthington, John D. and Moriel, Philipe}, year={2022}, month={Feb} }
 @article{alexander_pickworth_moore_poore_2020, title={102 Impact of weaning strategy on beef cow-calf behavior and activity}, volume={98}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skz397.141}, DOI={10.1093/jas/skz397.141}, abstractNote={Abstract
               The objective of this study was to investigate the impact of three weaning strategies on cow and calf behavior and activity. Research has shown that weaning imposes stress on both the cow and the calf that can alter behavior, activity, and growth performance. Angus-based cow-calf pairs (n = 147) were randomly assigned to one of three treatment groups, abrupt weaning on d0 (Abrupt, n=50), fence-line contact for 7 days and then relocation of the cow on d7 (Fence, n=50), and late weaning by abrupt separation on d84 (Late, n = 47). Both cow and calf behaviors were observed over 7d prior to weaning and 7d post weaning activity for each treatment group. A single observer recorded activity and vocalizations for each animal twice in morning and evening on observation days. Calf activity was also continuously measured using accelerometers secured to collars worn by five calves per treatment group. Observation and activity data were analyzed using PROC MIXED of SAS. Significance was determined at P < 0.05 and a tendency at 0.05< P ≤0.10. The average activity units per day (AU/d) prior to weaning were similar among treatment groups. On d1 of weaning, Fence calves, who still had a visual contact with their dam, tended to have greater AU/d (P = 0.09) than the Abrupt calves and maintained a higher level of activity through d14 (P < 0.001). Late calves tended to have greater AU/d (P = 0.10) than Abrupt or Fence on d0-1 after their weaning. No difference in vocalizations (P = 0.41) by calves 7d post weaning were observed (1% Abrupt, 0% Fence, and 4% Late). More cows on Fence paced (1%) than Abrupt (0%) or Late (0%) (P < 0.05) in the 7d of fenceline contact. Late cows (2%) vocalized more than Abrupt (1%) or Fence (0%) when their calves were still with them (P < 0.05), but not after (P > 0.05) when no vocalizations were recorded. These results differ from the previous year study; therefore, further investigation of the impact of weaning strategy on cow-calf behavior and activity is warranted.}, number={Supplement_2}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Alexander, Molly and Pickworth, Carrie L and Moore, Mikayla and Poore, Matt H}, year={2020}, month={Nov}, pages={62–62} }
 @article{brown_haimon_poole_poore_poole_2020, title={Changes in Cytokine Profiles in Response to Ergovaline Exposure in Slick vs. Normal Hair Coat in Beef Heifers.}, volume={98}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz397.091}, abstractNote={Abstract
               Fescue toxicosis is a syndrome common in cattle grazing fescue containing an endophyte that produces ergot alkaloids. Our lab has previously demonstrated that chronic exposure of ergot alkaloids adversely effects innate immune response. Therefore, the objective of this study was to examine changes in cytokine profiles in heat tolerant heifers exposed to ergot alkaloids from endophyte-infected fescue compared to wildtype controls. Angus X Senepol heifers were blocked by weight and hair coat genotype, Slick (S) or Normal (N) then randomly fed novel endophyte fescue (EN) or endophyte-infected fescue (EI) haylage in a total mixed ration for 63d (n=5 per group). Weekly measurements were collected to monitor physiological responses during exposure to ergot alkaloids. Blood samples were collected for cytokine analysis on d 0, 14, 28 and 56. Cytokine concentrations were quantified using Quantibody® Bovine Cytokine Arrays. Data were analyzed using PROC MIXED of SAS with repeated measures. Statistical significance was determined at P< 0.05 and a tendency at 0.05< P< 0.10. Concentrations of TNF were greater in EN-S heifers (6.57 ng/ml) compared to other heifer groups (P< 0.05; 1.33, 2.34, 3.15 ng/ml for EN-N, EI-S, and EI-N, respectively). Concentrations of IL21 were greater in EN-S heifers (8.15 ng/ml) compared to other heifer groups (P< 0.05; 1.55, 3.43, 3.26 ng/ml for EN-N, EI-S, and EI-N, respectively). Concentrations of IFNA (P=0.0543), IFNG (P=0.0558), IL1F1 (P=0.0587), and IL1F5 (P=0.0738) tended to be greater in in EN-S heifers compared to other heifer groups. Interestingly, concentrations of chemokine CCL4 (953.5 vs. 183.7 pg/mL) and CXCL9 (2.57 vs. 0.69 ng/mL) were significantly greater in S heifers than N heifers (P< 0.05). No differences were observed for IL13 and CXCL10 (P >0.05). In contrast to previous data, exposure to ergovaline via endophyte-infected fescue, did not induce an innate immune response. Rather, cytokine concentrations were elevated in heifers with the slick hair genotype. Overall, developing a better understanding of the interaction between ergot alkaloids and the slick hair genotype will help to improve cattle health management.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Brown, Alecia R. and Haimon, McKenzie L. and Poole, Rebecca and Poore, Matt H. and Poole, Daniel H.}, year={2020}, month={Nov}, pages={40–40} }
 @article{poole_mayberry_newsome_poole_galliou_khanal_poore_serao_2020, title={Evaluation of Resistance to Fescue Toxicosis in Purebred Angus Cattle Utilizing Animal Performance and Cytokine Response}, volume={12}, ISSN={["2072-6651"]}, DOI={10.3390/toxins12120796}, abstractNote={Fescue toxicosis is a multifaceted syndrome common in cattle grazing endophyte-infected tall fescue; however, varying symptomatic responses potentially imply genetic tolerance to the syndrome. It was hypothesized that a subpopulation of animals within a herd would develop tolerance to ergot alkaloid toxicity. Therefore, the goals of this study were to develop selection criteria to identify tolerant and susceptible animals within a herd based on animal performance, and then examine responsive phenotypic and cytokine profiles to fescue toxicosis. Angus cows grazed endophyte-infected tall fescue at two locations for 13 weeks starting in mid-April 2016. Forage measurements were collected to evaluate ergot alkaloid exposure during the study. A post hoc analysis of animal performance was utilized to designate cattle into either tolerant or susceptible groups, and weekly physiological measurements and blood samples were collected to evaluate responses to chronic exposure to endophyte-infected tall fescue. Findings from this study support the proposed fescue toxicosis selection method formulated herein, could accurately distinguish between tolerant and susceptible animals based on the performance parameters in cattle chronically exposed to ergot alkaloids, and provides evidence to warrant additional analysis to examine the impact of ergot alkaloids on immune responsiveness in cattle experiencing fescue toxicosis.}, number={12}, journal={TOXINS}, author={Poole, Daniel H. and Mayberry, Kyle J. and Newsome, McKayla and Poole, Rebecca K. and Galliou, Justine M. and Khanal, Piush and Poore, Matthew H. and Serao, Nick V. L.}, year={2020}, month={Dec} }
 @article{galliou_khanal_mayberry_poore_poole_serao_2020, title={Evaluation of a commercial genetic test for fescue toxicosis in pregnant Angus beef cattle}, volume={4}, ISSN={["2573-2102"]}, DOI={10.1093/tas/txaa181}, abstractNote={Abstract
               Most tall fescue [Lolium arundinaceum (Schreb.) Darbysh] in the Southeastern United States contains an endophyte that causes fescue toxicosis (FT) in grazing animals, a serious disease that causes approximately $1 billion in economic losses to the animal industries in the United States. Recently, a genetic test called T-Snip (AgBotanica, LCC, Columbia, MO), was developed with the objective of identifying animals with genetic variation for FT tolerance. The aim of this study was to validate the use of this genetic test in mature, pregnant cows. Over 13 wk, weekly phenotypic data, including body weight, rectal temperatures, hair coat scores, hair shedding scores, and body condition scores, were collected on 148 pregnant purebred Angus cows at 2 locations in NC where infected fescue was the primary source of feed. Birth weights (cBW) and 205-d adjusted weaning weights (adjWW) from these cow’s calves were recorded. All cows were genotyped for T-Snip. At the end of the trial, each phenotypic trait was calculated as the slope of the linear regression of performance on weeks. The effect of T-Snip rating genotypes (4 levels) on slope traits was tested using a linear model also including the fixed-effects of location, parity, and the initial measurement for each trait (covariate). For cBW and adjWW, the model also included the sex of the calf and the month of birth as categorical effects. Associations of T-Snip genotypes were observed for body weight gain (aBWd) of pregnant cows (P = 0.15; interaction with location), change in body condition score (aBCSd; P = 0.13), and adjWW (P = 0.06; interaction with location). For aBWd and adjWW, associations were found just within one location (P = 0.017 and 0.047, respectively), which was the location with higher endophyte infection rate. For all associations, the direction of the T-Snip genotypes was the same and as expected: the greater the genotype score, the better performance. No associations were found for the other traits (P > 0.10). These results indicate that the T-Snip test may be predictive of cow performance (aBWd, aBCSd, and adjWW) in an endophyte-infected tall fescue environment.}, number={4}, journal={TRANSLATIONAL ANIMAL SCIENCE}, author={Galliou, Justine M. and Khanal, Piush and Mayberry, Kyle and Poore, Matt H. and Poole, Daniel H. and Serao, Nick V. L.}, year={2020}, month={Oct} }
 @article{moore_cox-o'neill_poore_pickworth_2020, title={Impact of weaning strategy on beef cow and calf weight and body condition}, volume={98}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz397.192}, abstractNote={Abstract
               Weaning is one of the largest stressors in an animal’s life and can have long term impacts on the animal. The objective of this study was to investigate the effect of weaning strategy on the weight and body condition of beef cattle. A total of 249 Angus-based pairs at two locations were assigned to one of three treatments: abrupt removal of the calf on d0 (Abrupt, n = 84), fenceline contact for 7 days followed by complete separation on d7 (Fence, n = 85), or late weaning d84 (Late, n = 80). Weaned calves were supplemented 0.23 kg/hd/d of grain as fed while Late were not supplemented. Weights were obtained on d0, 7, 14, 28, 56, 84, 91, and 112. Body condition scores were recorded monthly. Data were analyzed using PROC MIXED of SAS and weights were analyzed with repeated measures. Significance was determined at P < 0.05. During the initial week post-abrupt weaning, Abrupt calves lost weight, Fence maintained weight, and Late calves gained weight (-0.4, 0, and 0.7 kg/d, respectively; P < 0.05). Over the complete weaning period (d0 to 112), Abrupt and Late weaned differed (P < 0.05) in their gains, while Fence was not different (P > 0.05) than either treatment (0.58, 0.64, and 0.62 kg/d, respectively). Cow overall ADG was not affected by weaning treatment (1.2, 0.8, and 0.5 kg/d, respectively; P >0.05). From d28 to 84, lactating Late cows gained less weight than Abrupt and Fence (0.3, 0.8, 0.8 kg/d, respectively; P < 0.0001). This contributed to a -0.1 BCS decrease at d112 for Late compared to a 0.3 and 0.2 BCS gain for Abrupt and Fence, respectively (P < 0.0001). Cows weaning calves after 10 mo of lactation were lighter than cows weaned after 7 mo; however, calves remaining with their dam maintained growth rates similar to grain supplemented weaned calves on pasture. While treatments influenced cow measures, all remained within normal values, therefore late weaning may be an option for producers to sell heavier calves without grain supplementation if marketing older calves.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Moore, Mikayla and Cox-O'Neill, Jordan and Poore, Matt H. and Pickworth, Carrie L.}, year={2020}, month={Nov}, pages={81–82} }
 @article{moore_cox-o'neill_poore_pickworth_2020, title={Impact of weaning strategy on beef cow and calf weight and body condition.}, volume={98}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz397.119}, abstractNote={Abstract
               Weaning is one of the largest stressors in an animal’s life and can have long term impacts on the animal. The objective of this study was to investigate the effect of weaning strategy on the weight and body condition of beef cattle. A total of 249 Angus-based pairs at two locations were assigned to one of three treatments: abrupt removal of the calf on d0 (Abrupt, n = 84), fenceline contact for 7 days followed by complete separation on d7 (Fence, n = 85), or late weaning d84 (Late, n = 80). Weaned calves were supplemented 0.23 kg/hd/d of grain as fed while Late were not supplemented. Weights were obtained on d0, 7, 14, 28, 56, 84, 91, and 112. Body condition scores were recorded monthly. Data were analyzed using PROC MIXED of SAS and weights were analyzed with repeated measures. Significance was determined at P < 0.05. During the initial week post-abrupt weaning, Abrupt calves lost weight, Fence maintained weight, and Late calves gained weight (-0.4, 0, and 0.7 kg/d, respectively; P < 0.05). Over the complete weaning period (d0 to 112), Abrupt and Late weaned differed (P < 0.05) in their gains, while Fence was not different (P >0.05) than either treatment (0.58, 0.64, and 0.62 kg/d, respectively). Cow overall ADG was not affected by weaning treatment (1.2, 0.8, and 0.5 kg/d, respectively; P > 0.05). From d28 to 84, lactating Late cows gained less weight than Abrupt and Fence (0.3, 0.8, 0.8 kg/d, respectively; P < 0.0001). This contributed to a -0.1 BCS decrease at d112 for Late compared to a 0.3 and 0.2 BCS gain for Abrupt and Fence, respectively (P < 0.0001). Cows weaning calves after 10 mo of lactation were lighter than cows weaned after 7 mo; however calves remaining with their dam maintained growth rates similar to grain supplemented weaned calves on pasture. While treatments influenced cow measures, all remained within normal values, therefore late weaning may be an option for producers to sell heavier calves without grain supplementation if marketing older calves.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Moore, Mikayla and Cox-O'Neill, Jordan and Poore, Matt H. and Pickworth, Carrie L.}, year={2020}, month={Nov}, pages={52–52} }
 @article{haimon_brown_poore_poole_2020, title={In Utero Exposure of Ergot Alkaloids does not Impact Heifer Growth and Reproductive Performance.}, volume={98}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz397.101}, abstractNote={Abstract
               In utero exposure to toxins can alter fetal development and postnatal performance. The objective of this study was to investigate the effects of in utero exposure of ergot alkaloids via endophyte-infected (EI) fescue diets during gestation on heifer growth, onset of puberty, ovarian characteristics and AI pregnancy rates. Pregnant Angus and SimAngus cows grazed either endophyte-free (EF) or EI pastures on d90-180 of gestation. Assessment of puberty of heifer (n = 40) via measurement of BW, BCS, reproductive tract scores (RTS), pelvic area (PA) and weekly blood sample for progesterone (P4) analysis started at 10 mo of age. At 14 mos of age, all heifers were synchronized using the CoSynch+7d CIDR protocol and inseminated to examine reproductive measurements, follicle mapping and AI pregnancy rate. Data were analyzed using a MIXED procedure of SAS and examined for effects of EI, sire, and age. Statistical significance was determined at P < 0.05 and a tendency at 0.05 < P < 0.10. In utero exposure to EI did not affect ADG, BW, or BCS (P > 0.05) however, there was a sire by treatment interaction for ADG during onset of puberty (P < 0.05). While EI did not affect PA or RTS (P > 0.05), sire significantly influenced RTS and PA (P < 0.05) thus impacting of onset of puberty. Based on P4 concentrations, only 55% of all heifers were cyclic by 14 months of age and there was a sire by treatment interaction for onset of puberty (P < 0.05). No differences were observes in AI pregnancy rate between heifer groups (EF 39.5±7; EI, 43.7±7; P >0.05). While EI did not affect follicle counts or luteal area (P > 0.05), sire significantly influenced the percentage of preselected (2-4mm) and selected (>9mm), but not transitional (5-8mm), ovarian follicles at breeding age (P < 0.05). While consumption of EI fescue during gestation impacts cow performance and onset of puberty in heifers, it has minimal effect on heifer growth and reproductive development and performance.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Haimon, McKenzie L. and Brown, Alecia R. and Poore, Matt H. and Poole, Daniel H.}, year={2020}, month={Nov}, pages={44–44} }
 @article{freeman_poore_pickworth_alley_2021, title={Influence of weaning strategy on behavior, humoral indicators of stress, growth, and carcass characteristics}, volume={5}, ISSN={["2573-2102"]}, DOI={10.1093/tas/txaa231}, abstractNote={Abstract
               Weaning is one of the most stressful events a calf experiences in our current beef production system. Its effects may include reduced feed intake, increased activity, slower growth, and increased susceptibility to disease. This study was designed to evaluate weaning after a 7-d placement of nose flaps at 7 mo (N, n = 40) and delaying weaning by 49 d relative to 7-mo weaning (D, n = 39) as alternatives to the industry standard; abrupt weaning at 7 mo of age (A, n = 39). The 4-yr trial utilized Angus and Angus X Senepol steer calves. Calves were randomly assigned to weaning strategy after being stratified by dam parity (heifer/cow), hair coat phenotype (normal/slick), and body weight. Behavioral observations were made on five steers per strategy group per year over the weeks surrounding weaning. Activity levels were determined by accelerometers worn on neck collars. Blood samples were obtained from the observed cattle during the last 2 yr to determine haptoglobin and cortisol concentration. Once weaned, the steers were followed through finishing and carcass characteristics obtained at harvest. Twelve of 38 steers in the N group had sores in their nostrils from the nose flaps when the flaps were removed at weaning of A/N (237 ± 3 d of age). The A and D calves were more active than N calves in the first 2 to 3 d after weaning but settled down to similar activity levels to N by the day 4. The A and D groups were more vocal than N during the same time frame. Cortisol and haptoglobin remained within normal reference ranges. Average daily body weight gain (ADG) was greater for D than A, who in turn had greater ADG than N during the first 42 d after A and N calves were weaned (0.69, 0.54, and 0.37 kg/d for D, A, and N, respectively; P < 0.01). All treatment groups graded Low Choice at harvest and exhibited similar efficiency of gain during growth and finishing (P > 0.2). Based on ADG during the 42 d after weaning, we recommend delaying weaning when available pasture and cow body condition support this strategy. When conditions do not permit delayed weaning, abrupt weaning may be the next viable option based on animal welfare concerns and increased handling to place and remove the flaps. Nose flaps reduced vocalization at weaning but resulted in less postweaning ADG. Based on our data, we suggest that abrupt weaning under the conditions of this study, is less stressful than we perceive it to be, based on calf behavior.}, number={1}, journal={Translational Animal Science}, author={Freeman, S. and Poore, M. and Pickworth, C. and Alley, M.}, year={2021}, month={Jan}, pages={txaa231} }
 @article{franzluebbers_poore_2021, title={Nutritive value of fall-stockpiled tall fescue pastures on southeastern US farms}, volume={113}, ISSN={["1435-0645"]}, DOI={10.1002/agj2.20517}, abstractNote={AbstractStockpiling of tall fescue [Schedonorus arundinaceus (Schreb.) Dumort.] may be a useful strategy to avoid fescue toxicosis in autumn and to reduce winter hay feeding costs of beef cattle grazing systems. Typical recommendations are to fertilize with N prior to fall growth. However, little information is available on how soil and pasture conditions affect forage nutritive value responses to fall fertilization. We analyzed the nutritive value of fall‐stockpiled forage in response to N fertilization on 92 fields in North Carolina and neighboring states from 2015 to 2018. We hypothesized that inherent soil nutrient cycling might minimize the need for exogenous N fertilizer inputs. Increasing N fertilization led to (a) greater crude protein, macronutrient concentrations (i.e., K, Ca, and P), and relative feed value and (b) lower acid detergent fiber, neutral detergent fiber, and lignin. However, changes were small, and effects were modified by surface soil properties. For example, crude protein increased from an average of 114 g kg–1 without N fertilization to 131 g kg–1 with 100 kg N ha–1, but this significant N fertilization effect diminished with increasing total organic C and inorganic N of a field. Small changes in nutritive value may have been a consequence of trials conducted on well‐managed farms with relatively high soil‐test biological activity and net N mineralization potential. Therefore, a single recommendation for fall N fertilization may not always be appropriate for enhancing nutritive value. Stockpiled tall fescue had sufficient nutritive value for pregnant cows, and N fertilization may not always be necessary.}, number={1}, journal={AGRONOMY JOURNAL}, author={Franzluebbers, Alan J. and Poore, Matt H.}, year={2021}, month={Jan}, pages={610–622} }
 @article{eisemann_ashwell_devine_poole_poore_linder_2020, title={Physiological response, function of sweat glands, and hair follicle cycling in cattle in response to fescue toxicosis and hair genotype}, volume={98}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skaa013}, DOI={10.1093/jas/skaa013}, abstractNote={AbstractFescue toxicosis is a syndrome that results when cattle consume toxic endophyte-infected tall fescue. The objective of this study was to compare the response in physiological variables, sweat gland function, hair follicle cycling, and gene expression to feeding a total mixed ration that included tall fescue haylage and tall fescue seed containing a toxic endophyte (EI) or tall fescue haylage containing a nontoxic novel endophyte (EN) in beef heifers (Angus × Senepol heifers, n = 31) with 2 different hair genotypes. Numbers in each subgroup were as follows: novel endophyte, heterozygous slick (EN-S; n = 8), novel endophyte, homozygous hairy (wild type, EN-W; n = 7), endophyte-infected, heterozygous slick (EI-S; n = 10), and endophyte-infected, homozygous hairy (wild type, EI-W; n = 6). Physiological measurements were taken weekly for 7 wk. Data were analyzed using the MIXED procedure of SAS including dietary fescue treatment (EN vs. EI) and hair genotype (S vs. W) as main effects, day as a repeated measure, and temperature–humidity index (THI) as a covariate. Skin biopsies were taken before treatment initiation and on day 37 of treatment. Average surface temperature (ST) increased as the THI increased (P < 0.0001). Average ST was greater (P < 0.01) for animals fed EI than for animals fed the EN fescue diet, and greater (P < 0.01) for animals with the W genotype compared with animals with the S genotype. The difference between heifers with the S and W genotype was greater at greater THI (genotype × day interaction, P < 0.01). Transepidermal water loss (TEWL) was greater (P < 0.05) for animals with the S genotype compared with the W genotype and greater (P < 0.05) for heifers with the S genotype than for heifers with the W genotype when fed EI (36.7, 38.5, 30.0, and 38.7 g/m2 per hour for EN-W, EN-S, EI-W, and EI-S, respectively). The fraction of follicles in telogen in plucked hair samples for heifers fed EI was greater for animals with the S genotype than the W genotype (fraction in telogen: 0.456, 0.565, 0.297, 0.702 for EN-W, EN-S, EI-W, and EI-S, respectively; diet × genotype interaction, P < 0.05). Fraction of follicles in anagen was the opposite. EI fescue resulted in increased ST, changes in hair follicle cycling that support greater hair growth, and decreased TEWL for heifers with the W genotype compared with S genotype, suggesting greater heat stress in response to EI.}, number={3}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Eisemann, J.H. and Ashwell, M.S. and Devine, T.L. and Poole, D.H. and Poore, M.H. and Linder, K.}, year={2020}, month={Mar} }
 @article{franzluebbers_poore_freeman_rogers_2021, title={Soil nutrient distribution on cattle farms in three physiographic regions of North Carolina}, volume={113}, ISSN={["1435-0645"]}, DOI={10.1002/agj2.20508}, abstractNote={AbstractBeef cattle production systems are sometimes considered a source of nutrient enrichment due to alteration of botanical composition and excretion of nutrients in isolated zones of pastures. However, research on environmental effects from livestock grazing in the eastern United States has been scant. We conducted an intensive soil sampling campaign on six private farms in three physiographic regions of North Carolina to determine the impacts of landscape features, pasture management, and animal behavior on a suite of soil nutrients. Total and particulate organic C and soil‐test biological activity (STBA) increased with increasing elevation due to edaphic and climate factors. Within farms, fields with different management illustrated that: (a) swine and poultry manure application can favorably enrich soil with infrequent application, but lead to excessive soil‐test P and K with repeated application; (b) positive impacts on total and particulate organic C and STBA occur with grazed perennial forages compared with annual forages and previous cropping; and (c) enrichment of total and particulate organic C and STBA occurs with winter hay feeding, but excessive nutrient accumulation can occur if fed in the same location repeatedly. Aggregated enrichment of soil organic C, STBA, and soil‐test P and K occurred on 30% of fields near drinking water tanks, shade sources, gates, and hay feeding stations. Improved grazing management with rotational stocking and more year‐round grazing helped to minimize occurrence and magnitude of nutrient enrichment zones. We conclude that improved grazing management systems can have positive impacts on the environment, and likely on farm livelihoods.}, number={1}, journal={AGRONOMY JOURNAL}, author={Franzluebbers, Alan J. and Poore, Matt H. and Freeman, Sharon R. and Rogers, Johnny R.}, year={2021}, month={Jan}, pages={590–609} }
 @article{franzluebbers_poore_2020, title={Soil-test biological activity with the flush of CO2: VII. Validating nitrogen needs for fall-stockpiled forage}, volume={112}, ISSN={["1435-0645"]}, DOI={10.1002/agj2.20153}, abstractNote={AbstractNitrogen fertilizer is an expensive input, and its necessity in grazed tall fescue (Schedonorus arundinaceus) pastures can be questioned when sufficient soil N mineralization occurs. A soil‐testing tool that could predict the need for N fertilizer inputs would be beneficial to producers to optimize profit and avoid environmental contamination. Thirty‐seven on‐farm trials were conducted in the Piedmont and Blue Ridge regions of Georgia, South Carolina, North Carolina, and Virginia in 2018 to evaluate fall‐stockpiled tall fescue yield response to N and P fertilizer inputs. Carbon and N characteristics of the soil surface (0‐ to 10‐cm depth) and surface residue varied among sites. Forage mass responses to P fertilizer input were below the economic threshold, regardless of Mehlich‐III extractable P. Forage mass responses to N fertilizer input did not exceed a low cost/value threshold of 5 kg forage kg−1 N in 24 of the trials. Economically optimum N rate was greatest when soil N mineralization and soil‐test biological activity (STBA) were at low levels. Results validated those of an earlier study on 55 fields, and taken together, suggest that N fertilizer for fall‐stockpiled tall fescue could be as high as 80 kg N ha−1 on fields with very low STBA (<100 mg CO2–C kg−1 soil 3 d−1) and declining to nil with medium STBA (>250 mg CO2–C kg−1 soil 3 d−1), depending on cost/value threshold. Healthy soils with high STBA can be managed effectively without N fertilizer inputs to recycle nutrients and promote more sustainable agricultural systems.}, number={3}, journal={AGRONOMY JOURNAL}, author={Franzluebbers, Alan J. and Poore, Matt H.}, year={2020}, pages={2240–2255} }
 @article{phipps_harmon_poore_henderson_bryant_ashburn_shaeffer_rogers_2020, title={Utilizing craft brewing side streams of trub, hops, and yeast mix (THYM) as a novel feed additive and an alternative to monensin in beef cattle diets.}, volume={98}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz397.115}, abstractNote={Journal Article RETRACTED: 186 Utilizing craft brewing side streams of trub, hops, and yeast mix (THYM) as a novel feed additive and an alternative to monensin in beef cattle diets Get access Kendra P Phipps, Kendra P Phipps North Carolina State University Search for other works by this author on: Oxford Academic PubMed Google Scholar Deidre D Harmon, Deidre D Harmon North Carolina State University Search for other works by this author on: Oxford Academic PubMed Google Scholar Matt H Poore, Matt H Poore Department of Animal Science - North Carolina State University Search for other works by this author on: Oxford Academic PubMed Google Scholar Ethan Henderson, Ethan Henderson North Carolina Cooperative Extension Search for other works by this author on: Oxford Academic PubMed Google Scholar Robert Bryant, Robert Bryant Warren Wilson College Search for other works by this author on: Oxford Academic PubMed Google Scholar Brock Ashburn, Brock Ashburn Highland Brewing Company Search for other works by this author on: Oxford Academic PubMed Google Scholar April Shaeffer, April Shaeffer North Carolina State University Search for other works by this author on: Oxford Academic PubMed Google Scholar Johnny R Rogers Johnny R Rogers North Carolina State University Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Animal Science, Volume 98, Issue Supplement_2, November 2020, Page 50, https://doi.org/10.1093/jas/skz397.115 Published: 28 November 2020}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Phipps, Kendra P. and Harmon, Deidre D. and Poore, Matt H. and Henderson, Ethan and Bryant, Robert and Ashburn, Brock and Shaeffer, April and Rogers, Johnny R.}, year={2020}, month={Nov}, pages={50–50} }
 @article{phipps_harmon_poore_henderson_bryant_ashburn_shaeffer_rogers_2020, title={Utilizing craft brewing side streams of trub, hops, and yeast mix (THYM) as a novel feed additive and an alternative to monensin in beef cattle diets.}, volume={98}, ISBN={1525-3163}, DOI={10.1093/jas/skz397.021}, abstractNote={Abstract
               The objective of this experiment was to evaluate craft brewing side streams of trub, hops and yeast mix (THYM) as a potential feedstuff and replacement for monensin in growing beef cattle rations. British-cross steers (n = 45, initial BW = 732 ± 58kg) were stratified by BW and randomly assigned to 1 of 15 pens (3 head/pen). Treatment diets were randomly assigned to pen and consisted of a corn silage based total mixed ration (TMR) with no supplementation (CON; 70.99% TDN, 12.25% CP), supplementation with monensin (MON; 22g/907kg ration, dry matter basis) or supplementation with the brewery side streams (THYM; 70.95% TDN, 12.49% CP, 2.65% THYM, dry matter basis). Steers were adjusted to a TMR for 14 d prior to a 63 d collection period. Body weight and serum urea nitrogen (SUN) were measured on d 0 and every 21 d thereafter. Rumen fluid was collected via esophageal/rumen tube at d 42 and 63 for short chain fatty acid analysis (SCFA). Data were analyzed using the MIXED procedure of SAS 9.4 (Cary, NC) with treatment as the main effect and using repeated measures. Average daily gain and dry matter intake did not differ among treatments (P = 0.96, respectively), but a tendency was noted for THYM to have greater average gain:feed than the CON treatment. SUN concentrations were least on d 0 (P < 0.01) but did not differ among treatments at any time (P =0.11). On d 42, THYM had a greater (P = 0.05) A:P than MON. Concentrations of propionic and valeric acid were greater (P < 0.05) for CON than THYM on d 63, while SCFA concentrations tended to be greater (P = 0.08) for CON than THYM. THYM also tended to have a greater A:P on d 63 than CON. Interpretation of the data indicates that inclusion of THYM in growing cattle rations has no negative impact on animal performance.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Phipps, Kendra P. and Harmon, Deidre D. and Poore, Matt H. and Henderson, Ethan and Bryant, Robert and Ashburn, Brock and Shaeffer, April and Rogers, Johnny R.}, year={2020}, month={Nov}, pages={9–10} }
 @article{carvalho_sanglard_nascimento_moriel_sommer_merrill_poore_duarte_serao_2020, title={miRNAs explain the variation in muscle and blood transcriptomes of beef calves born from dams with or without energy restriction during late gestation}, volume={98}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skaa054.292}, abstractNote={Abstract
               Maternal energy restriction during late gestation affects the expression of genes related to energy metabolism in muscle and immune response in blood. MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally. The aim of this study was to identify potentials miRNA involved in the expression of differentially expressed genes (DEG) in muscle and blood following exposure to maternal gestational energy restriction. Forty days before the expected calving date, cows were assigned to one of two diets: 100% (CTRL) or 70% (REST) of the daily energy requirement. For RNA-seq analysis, muscle samples were collected from 12 heifers and 12 steers, and blood samples were collected from 12 steers. miRNAs were identified from the RNA-seq data based on the bovine genome annotation, with 38 and 10 miRNAs identified in blood and muscle, respectively. The expression of the miRNAs and the previously identified 160 and 450 DEGs in muscle and blood, respectively, was pre-adjusted for fixed effects before final analyses. A stepwise selection (P-value < 0.05) was used to identify miRNAs (dependent variables) explaining variation in DEGs, for each DEG at a time, and analyses performed separately for blood and muscle. The R2 of selected models ranged from 0.88 to 0.99 in muscle and 0.92 to 0.99 in blood. Of the most selected miRNA in muscle, MiR-133a and MiR-1 are known to be related to muscle hypertrophy, and MiR-143 and bta-let-7i promote adipocyte differentiation. Of the most selected miRNA in blood, MiR-21 regulates immune system by different pathways. Using RNA-seq data, we identified miRNAs explaining a large amount of the variation of DEGs, with the identification of important miRNAs related to muscle development and immune system.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Carvalho, Elisa B. and Sanglard, Leticia P. and Nascimento, Moyses and Moriel, Philipe and Sommer, Jeffrey and Merrill, Melissa and Poore, Matthew and Duarte, Marcio and Serao, Nick}, year={2020}, month={Nov}, pages={165–165} }
 @article{ott_freeman_poore_pickworth_2019, title={11 Impact of weaning strategy on calf performance, behavior, and activity}, volume={97}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skz053.027}, DOI={10.1093/jas/skz053.027}, abstractNote={Abstract
               Weaning is known to be the most stressful time in a beef calf’s post-natal life. Weaning stress may have long-term impacts on cattle performance. This study was conducted to determine differences in calf stress under three weaning strategies. Two hundred twenty-nine Angus or Angus-Simmental cross pairs at two locations were assigned to one of three weaning treatments: abrupt removal of the calf on d 0 (Abrupt, n = 77), fenceline contact for 7 d prior to removal of the calf on d 7 (Fence, n = 79), and abrupt removal of the calf on d 84 (Late, n = 73). Behavioral observations were conducted pre- and post-weaning and total activity was measured prior to and after weaning using accelerometers placed on collars on five calves per treatment. Calf weights, hip heights, and body condition scores (BCS) were collected to evaluate calf performance post-weaning. Data were analyzed using PROC MIXED of SAS. Statistical significance was determined at P < 0.05 and a tendency at 0.05<p<0.10. Skeletal growth via hip height was not affected by weaning treatment. During the first 28 d after initial weaning, the Abrupt calves lost while the Late weaned calves gained weight (-0.09 and 0.49 kg/d, respectively; P < 0.05). Fence were intermediate weight and tended to be different from both treatments (0.02 kg/d; P = 0.09). Overall ADG was not affected (P > 0.10) by timing or strategy of weaning. Total activity was greater (P < 0.05) in Abrupt than Fence on d 1 and d 2 post-wean (Abrupt 26772 and 22346 and Fence 22235 and 16691 relative activity units/hour, respectively). Calf activity was elevated (P < 0.05) on day of weaning until d 2 post-wean then returned to pre-wean level by d 3 for all treatments. Short-term differences in calf performance warrant further exploration of the possible long-term effects on heifer development and steer feedlot performance.}, number={Supplement_1}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Ott, Grace C and Freeman, Sharon R and Poore, Matthew H and Pickworth, Carrie L}, year={2019}, month={Jul}, pages={12–13} }
 @article{brown_poole_jackson_poore_pickworth_poole_2019, title={111 Changes in proinflammatory cytokines and chemokines in beef steers chronically exposed to endophyte-infected tall fescue seed}, volume={97}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skz053.095}, DOI={10.1093/jas/skz053.095}, abstractNote={Abstract
               Endophyte-infected tall fescue consumption adversely effects health and immune response. The aim of this study was to evaluate the impact of protein supplementation on cytokine response in steers consuming ergovaline, found in endophyte-infected tall fescue. Thirty-two beef steers were fed a total mixed ration (TMR) and randomly assigned to receive one of four treatments for 56 days: endophyte-free seed (0 ug/kg ergovaline) with either 18% (EF-18; n = 8) or 14% dietary protein (EF-14; n = 8) and endophyte-infected seed (500 ug/ kg ergovaline) with 18% (EI-18; n = 8) or 14% dietary protein (EI-14; n = 8). Blood samples were collected for cytokine analysis on d 28 and d 42 of the feeding period representing when steers vaccinated and boostered for bovine viral diarrhea (BVD) types 1 and 2 and infectious bovine rhinotracheitis (IBR). Cytokine concentrations were evaluated using Quantibody Bovine Cytokine Arrays. Data were analyzed using PROC MIXED of SAS with repeated measures. Statistical significance was determined at P0.05). Interestingly, concentrations of cytokines, IL13 (912±148 vs. 410.9±135 pg/mL) and IL21 (1636.0±295 vs. 629±305 pg/mL), which are involved in chronic inflammatory process were greater (P < 0.05) in EI steer compared to EF steers on d 28 and d 42. Concentrations of chemokine CCL4 and proinflammatory cytokines IFNG and IL-1alpha tended to be greater in EI steers compared to EF steers (P < 0.10). Overall, these data demonstrate that consumption of ergovaline increased proinflammatory cytokines and induced a chronic immune response. Developing a better understanding of overall immunity in cattle exposed to endophyte-infected tall fescue is fundamental to improve cattle health within the beef industry.}, number={Supplement_1}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Brown, Alecia R and Poole, Rebecca K and Jackson, McKenzie Lane and Poore, Matthew H and Pickworth, Carrie L and Poole, Daniel H}, year={2019}, month={Jul}, pages={42–43} }
 @article{davis_poole_poore_poole_pickworth_2019, title={47 Interaction of dietary protein and endophyte- infected Tall Fescue seed in stocker steers on finishing phase performance and carcass traits}, volume={97}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skz053.189}, DOI={10.1093/jas/skz053.189}, abstractNote={Abstract
               Fescue toxicosis is a multifaceted disease that negatively impacts cattle. It was hypothesized that exposure to endophyte-infected fescue during the stocker phase affects finishing phase growth and carcass characteristics. Eight-month-old steers were sorted by weight and randomly assigned to receive one of four treatments for 56 days in confinement: endophyte-free seed (0 ug/kg ergovaline) with either 18% (EF-18; n = 9) or 14% dietary protein (EF-14; n = 9) and endophyte-infected seed (500 ug/kg ergovaline) with 18% (EI-18; n = 9) or 14% dietary protein (EI-14; n = 9). Following fescue exposure calves were backgrounded on novel endophyte fescue pastures for 8 mo. At 18 mo, all steers were vaccinated, implanted, and reentered the feedlot for a 112 d growing phase and 52 d finishing phase. Body weight was recorded every 28 d and carcass data was collected at slaughter. Data were analyzed using the MIXED procedure of SAS for effects of BW, ADG, dressing percentage (DP), HCW, backfat, ribeye area (REA), KPH, USDA Yield grade (YG), and USDA Quality grade (QG). Statistical significance was determined at P > 0.05) HCW, BW, backfat, REA, DP, or QG. TheEI-14 had a greater ADG (1.69 kg/d) during the feedlot growing phase compared to the other treatment groups (1.53, 1.47, 1.55 for EI-18, EF-14, EF-18, respectively; P > 0.05). Steers stockered on 18% dietary protein had greater BW compared to steers previously fed at 14% (501.3±3.8 vs. 489.7±3.8; P = 0.0339). In addition, 18% protein in stocker diet resulted in greater KPH (2.3±0.2 vs. 1.78±0.2; P = 0.0129) and YG (3.4±0.1 vs. 3.0±0.1; P = 0.0168) compared with steers fed 14% dietary protein. Finishing growth and carcass characteristics were not affected by exposure to ergot alkaloids at weaning as much as dietary protein when cattle had a background period prior to feedlot entry}, number={Supplement_1}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Davis, Matthew E and Poole, Rebecca K and Poore, Matthew H and Poole, Daniel H and Pickworth, Carrie L}, year={2019}, month={Jul}, pages={83–83} }
 @article{worthington_ott_poore_pickworth_2019, title={57 Impact of weaning strategy on beef cow performance}, volume={97}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skz053.000}, DOI={10.1093/jas/skz053.000}, abstractNote={Abstract
               It has been shown that physiologic stress in beef cattle can impact animal performance. Weaning stress is recognized as a major stress in a calf’s life. However, less is known of its impacts on the dam or developing fetus, as the dam is at the end of the second trimester at weaning. Therefore, the objective was to investigate the impact of weaning stress on cow performance. A total of 240 cows from two locations were assigned to one of three weaning strategies: abrupt removal of the calf on d 0 (n = 80), d 7 cow-calf fenceline contact (n = 81), and late weaning on d 84 (n = 79). Cows averaged 16 and 24 weeks of gestation on d 0 and d 84 respectively. Weights and BCS were obtained monthly. Behavioral observations occurred over the week before and after weaning for each weaning period. Five late-wean cows per location were milked on d 14 and d 70 to estimate milk yield throughout the late wean period. Data were analyzed using the MIXED procedure of SAS with BW and BCS utilizing repeated measures. Means are declared different at P ≤ 0.05. The ADG over the course of the study was lower (P < 0.05) for the late weaned cows compared to the abrupt weaned cows. This was also reflected by change in body condition where late weaned cows dropped from a 5.7 to a 4.9 (P < 0.05) over the 105 d. Milk production in late weaned cows decreased over time (6.0 and 4.4 kg/d for d 14 and 70, respectively; P = 0.05). Energy expenditure for lactation may have contributed to the drop in BCS of the late weaned compared with the non-lactating cows and could compromise third trimester growth of calf in utero. The results of this study warrant further exploration of the effect of weaning stress on dams and their developing fetuses.}, number={Supplement_1}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Worthington, Mary Clarke and Ott, Grace C and Poore, Matthew H and Pickworth, Carrie L}, year={2019}, month={Jul}, pages={1–1} }
 @article{poole_womble_poore_poole_pickworth_2019, title={Effects of endophyte-infected tall fescue seed and protein supplementation on stocker steers: I. Growth performance and hemodynamic responses}, volume={97}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skz249}, DOI={10.1093/jas/skz249}, abstractNote={Abstract
               Fescue toxicosis is a multifaceted syndrome common in cattle grazing endophyte-infected tall fescue and is detrimental to growth and performance. Recent research has shown that supplementing protein has the potential to enhance growth performance in weaned steers. Therefore, the objective of this study was to evaluate the effect of supplemental CP on physiological parameters in stocker steers experiencing fescue toxicosis. Thirty-six weaned Angus steers (6 mo of age) stratified by weight (196.1 ± 3.6 kg) were assigned to a 2 × 2 factorial arrangement for 56 d: endophyte-free (EF) seed and 14% CP (EF-14; n = 9), EF seed and 18% CP (EF-18; n = 9), endophyte-infected (EI) seed and 14% CP (EI-14; n = 9), and EI seed and 18% CP (EI-18; n = 9). Steer growth and hemodynamic responses were collected weekly during ergot alkaloid exposure. On day 14 of the trial, iButton temperature data loggers were subcutaneously inserted in the lateral neck region to record hourly body temperature for 42 d. Data were analyzed using PROC MIXED of SAS with repeated measures. No differences were observed in DMI, BW, ADG, F:G, or BCS during the treatment period (P > 0.05). Hair shedding scores, rectal temperatures, surface temperatures, and respiration rates were greater in EI steers compared to EF steers regardless of supplemental CP (P < 0.05). However, subcutaneous body temperature was greater in EI-14 steers (37.94 °C) compared to other steer groups (37.60, 37.68, 37.72 ± 0.04 °C for EF-14, EF-18, and EI-18, respectively; P < 0.05). Prolactin concentrations tended to be greater in EF steers when compared to EI steers (P = 0.07). Heart rate and hematocrit were reduced for EI-18 steers compared to other steer groups (P < 0.05). Caudal artery diameter was reduced in EI-18 steers compared to EI-14 steers (2.60 vs. 2.75 ± 0.05 mm, respectively; P < 0.05) and caudal vein diameter was reduced in EI-18 steers (3.20 mm) compared to all other steer groups (3.36, 3.39, 3.50 mm for EF-14, EF-18, and EI-14, respectively; P < 0.05). However, there was no difference observed in systolic or diastolic blood pressure during the treatment period (P > 0.05). Based on the data, exposure to low to moderate levels of ergot alkaloids during the stocker phase had a negative impact on hemodynamic responses and supplemental CP had minimal impact to alleviate symptoms. Therefore, feeding additional protein above established requirements is not expected to help alleviate fescue toxicosis.}, number={9}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Poole, Rebecca K and Womble, Carrisa M and Poore, Matthew H and Poole, Daniel H and Pickworth, Carrie L}, year={2019}, month={Jul}, pages={3776–3785} }
 @article{poole_brown_poore_pickworth_poole_2019, title={Effects of endophyte-infected tall fescue seed and protein supplementation on stocker steers: II. Adaptive and innate immune function}, volume={97}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skz250}, DOI={10.1093/jas/skz250}, abstractNote={AbstractFescue toxicosis is a multifaceted syndrome common in cattle grazing endophyte-infected tall fescue that affects performance; however, little information is available pertaining to its effects on immunity. Recently, it has been shown that supplemental CP can improve performance in weaned steers postvaccination. Thus, the objective of this study was to evaluate the effect of supplemental CP on innate and adaptive immune responses in stocker steers chronically exposed to ergovaline. Angus steers (n = 12 pens; 3 steers/pen) were stratified by weight and assigned to a 2 × 2 factorial arrangement to examine crude protein levels of supplement (14% or 18%) and ergovaline exposure (0 or 185 μg ergovaline/kg BW/d via ground endophyte-free (EF) or endophyte-infected (EI) tall fescue seed, respectively) on immune response. Consumption of low to moderate concentration of ergovaline from EI tall fescue seed was sufficient to induce mild symptoms associated with fescue toxicosis. Blood samples were collected at day 0, 42, and 56 to evaluate infectious bovine rhinotracheitis (IBR) and bovine viral diarrhea virus (BVDV) type 1b titers following vaccine challenge. Additionally, serum cytokine concentrations were evaluated using Quantibody Bovine Cytokine Arrays on day 0, 28, and 42. Data were analyzed using PROC MIXED of SAS with repeated measures. Regardless of treatment, no differences were observed in IBR and BVDV-1b seroconversion following vaccine challenge (P > 0.05). Regardless of crude protein concentration, EI steers had greater concentrations of proinflammatory cytokines (TNF-α, IFN-γ, IL-1α), chemokines (CCL2, CCL4, MIG), anti-inflammatory cytokines (IL-2, -13, -15, -21), and various growth factors (FGF-1, IGF-1, VEGF-A) when compared to EF steers (P < 0.05). Furthermore, VEGF-A and IGF-1 concentrations were greater in EI-14 steers on day 28 compared to EI-18, EF-14, and EF-18 steers (P < 0.05), however, this difference was not observed on day 0 or 42 (P > 0.05). Based on these data, steers exposed to ergovaline have an increase in pro- and anti-inflammatory cytokines and supplemental CP had minimal impact to mitigate this response. However, in the current study, exposure to ergovaline had little to no effect on adaptive immunity and response to vaccination. Together, chronic exposure to ergovaline results in a hyperactive innate immune response, which may lead to an immuno-compromised animal.}, number={10}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Poole, Rebecca K and Brown, Alecia R and Poore, Matthew H and Pickworth, Carrie L and Poole, Daniel H}, year={2019}, month={Jul}, pages={4160–4170} }
 @article{poore_rogers_franzluebbers_2019, title={Enhancing production efficiency on southern beef farms with an interactive extension approach using forage management.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.158}, abstractNote={Abstract
               Production efficiency in beef grazing systems can be improved by several relatively simple changes in forage management practices. During a 4-year project period funded by the NRCS Conservation Innovation Grant Program (68-3A75-14–251) and in collaboration with the University of Georgia, the Amazing Grazing Program in North Carolina utilized several innovative forage management approaches on 6 private farms and 3 public research stations to educate beef producers. On-farm grid sampling illustrated spatial distribution of soil nutrients as influenced by long-term historical management, particularly of winter hay feeding stations. Side-by-side testing of simple and complex annual forage mixes on three private farms demonstrated the opportunities to enhance forage nutritive value, capture the value of diverse botanical composition, and to defer grazing on perennial pastures. Smallplot testing of yield response to fall-stockpiled tall fescue nitrogen fertilizer rate allowed producers to reap the economic benefits of improved soil health and to help improve the environment surrounding their farm. At each private farm, summer, and winter forage management workshops explored innovative approaches to beef farm management, including the nature and importance of dung beetles, value of simple compared with complex annual forages, how to approach winter grazing, benefits of hay unrolling, need for mineral supplementation, utilizing temporary fencing, and frost-seeding clovers. As the project progressed, interest and attendance at workshops grew and cooperating farmer engagement increased. We found that many small-scale beef farmers were interested in simple practices such as hay unrolling and frost seeding when demonstrated by their peers. Using a variety of educational approaches, workshop audiences responded positively to hands-on activities and time for open discussion. Attendees were interested, attentive, and motivated to adopt new practices based on these practical demonstrations on peer farms.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poore, Matthew H. and Rogers, Johnny R. and Franzluebbers, Alan J.}, year={2019}, month={Jul}, pages={70–70} }
 @article{freeman_poore_shaeffer_2019, title={Enhancing the adoption of stockpiling tall fescue and managed grazing}, volume={3}, ISSN={["2573-2102"]}, DOI={10.1093/tas/txz086}, abstractNote={Abstract
               One of the primary goals of extension is to encourage and support adoption of improved technologies. Managed grazing and stockpiling forage are two such technologies. The goal of this project was to encourage adoption of these practices by providing on-farm demonstrations of the technologies. We also collected forage and soil data and held workshops at each of the demonstration sites. Host producers were selected, given a basic kit of temporary fencing supplies for use during the demonstration, and instructed on their proper use during the winter stockpiling season. Forage yield and quality data were collected and soil tests made to show the economic advantages of proper fertilization and grazing fresh forage in contrast to feeding stored forage and concentrate. The nutritive value of the forage stockpiled in September through November (67% total digestible nutrients [TDN] and 14% crude protein, CP) exceeded the needs of the cattle and was greater than the nutrient content of hay present on the farms (59% TDN and 11% CP, P < 0.01). The mean quantity of available forage (2,856 ± 164 kg dry matter per hectare) provided an average of 260 (±81.8) standard cow (545 kg) grazing days per hectare of stockpiled forage. Taking into account the higher nutritive value of the fresh forage when compared with hay and the savings of time and equipment costs by grazing, we estimated that grazing stockpiled forage saved $1.28 per standard cow per day. The grazing management skills gained during this project and the temporary fencing technology were adopted by 93% of the demonstration farms that responded to our follow-up survey (78% of demonstration sites), and the area managed with these technologies increased on these sites more than 350%. Having the hosts share personal experiences played an important role in encouraging their peers to adopt the technologies.}, number={4}, journal={TRANSLATIONAL ANIMAL SCIENCE}, author={Freeman, Sharon and Poore, Matthew and Shaeffer, April}, year={2019}, month={Jul}, pages={1099–1105} }
 @article{mayberry_poole_newsome_khanal_poore_serao_poole_2019, title={Evaluation of cytokine response as an indicator of genetic resistance to fescue toxicosis in purebred Angus cattle.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.102}, abstractNote={Abstract
               Fescue toxicosis is a multifaceted syndrome common in cattle grazing endophyte-infected tall fescue. However, varying responses among cattle potentially implies genetic tolerance to the syndrome. Therefore, the objective was to evaluate the cytokine response of cows deemed either tolerant or susceptible to endophyte-infected fescue. Angus cows, 2–4 yrs old, grazed endophyte-infected tall fescue at two locations for 13 weeks starting mid-April 2016. A post-hoc analysis of animal performance was utilized to designate cattle into either tolerant (n = 20) or susceptible (n = 20) groups, based on physiological responses (changes in ADG) to chronic exposure to endophyte-infected fescue. Blood samples collected on weeks 1, 7, and 13 were used to evaluate serum prolactin (PRL) concentration. Cytokine concentrations were evaluated via Quantibody Bovine Cytokine Arrays. Data were analyzed using PROC MIXED of SAS with repeated measures. There were no differences by location or week. Tolerant cows tended to have decreased overall PRL concentrations compared to susceptible cows (98.3 and 138.4 ng/mL, respectively; P = 0.08). G protein-coupled receptor associated sorting protein (GASP)-1, a down-regulator of G protein-coupled receptors including D2-dopamine receptor a regulator of PRL secretion, was greater in tolerant cows when compared to susceptible cows (4.7 and 4.2 ng/mL; P = 0.03). Vascular endothelial growth factor (VEGF)-A was greater in tolerant cows when compared to susceptible cows (0.5 and 0.2 ng/mL; P = 0.02). Finally, interleukin (IL)-4, an anti-inflammatory cytokine, tended to be greater in tolerant cows when compared to susceptible cows (2.2 and 0.8 ng/mL, respectively; P = 0.07). Based on these data, greater concentrations of GASP-1, VEGF-A, and IL-4 in tolerant compared to susceptible cattle may mitigate the detrimental effects of fescue toxicosis, including suppressed PRL and vasoconstriction, leading to improved animal performance in a fescue environment. Additionally, these cytokines may serve as potential biomarkers for determining an animal’s tolerance or susceptibility to negative effects associated with endophyte-infected tall fescue}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Mayberry, Kyle and Poole, Rebecca K. and Newsome, McKayla A. and Khanal, Piush and Poore, Matthew H. and Serao, Nick V. L. and Poole, Daniel H.}, year={2019}, month={Jul}, pages={45–46} }
 @article{khanal_sanglard_mayberry_sommer_poore_poole_serao_2019, title={Genes and functions associated with tolerance to fescue toxicosis in Angus cows}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz122.295}, abstractNote={Abstract
               The objective of this study was to identify differentially expressed genes (DEG) and functions associated with tolerance to fescue toxicosis (FT). Forty pregnant purebred Angus cows were selected based on their growth at two locations in North Carolina (Butner Beef Cattle Field Laboratory, BBFCL; Upper Piedmont Research Station, UPRS) and classified as either high tolerant (HT) or low tolerant (LT) to FT with 20 cows in each group balanced by location. Blood samples were collected on weeks 1, 5, 9, and 13 for RNA sequencing. Counts were analyzed using a negative binomial model including the effects of genetic group, location, time, all possible interactions of these effects, flow cell, covariate of RNA integrity number, and normalized library size as offset. Genotype-by-location-by-time interaction was evident with a high number (4,453) of DEG (q-value<0.1) between genetic groups on week 5 at UPRS compared to all other possible interactions. So further analyses were focused on week 5 at UPRS. The most significant upregulated genes in LT and HT animals were ENPP6 and MESP2, respectively, with log2 fold changes of 1.90 [95% confidence interval = 0.89, 2.92] q-value=0.005) and 0.91 [0.35, 1.47] (q-value=0.01), respectively. Other top 5 upregulated genes for HT animals were CTBS, CLDN19, SPDYC, HEYL, and SDC2, and for LT animals were OLIG1, IL13, ANXA13, ENSBTAG00000024188and CXCL13. Enrichment analysis (P < 0.05) showed that DEG between genetic groups have general functions, such as metabolic, biosynthetic, and catabolic processes, as well as DNA and RNA-related functions, such as translation, transcription, and repair. These findings helped characterizing the genetic basis of tolerance to FT in cattle. In addition, we identified genes that may serve as potential biomarkers for tolerance to FT}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Khanal, Piush and Sanglard, Leticia Pereira and Mayberry, Kyle and Sommer, Jeffrey and Poore, Matthew H. and Poole, Daniel H. and Serao, Nick V. L.}, year={2019}, month={Jul}, pages={167–167} }
 @article{poole_devine_mayberry_eisemann_poore_long_poole_2019, title={Impact of slick hair trait on physiological and reproductive performance in beef heifers consuming ergot alkaloids from endophyte-infected tall fescue}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz024}, abstractNote={Fescue toxicosis is a multifaceted syndrome common in cattle grazing endophyte-infected tall fescue. The objective of this study was to evaluate the impact of the slick hair trait on physiological and reproductive parameters in heifers experiencing fescue toxicosis. Angus × Senepol heifers (n = 31) were blocked by weight (393.5 ± 17.3 kg) and phenotype relative to hair coat at birth, and randomly fed novel endophyte fescue (EN) or endophyte-infected fescue (EI) haylage in a total mixed ration for 91 d. Weekly measurements were collected to monitor heifer growth and response during ergot alkaloids exposure. Following 28 d of treatment, estrus was synchronized and heifers were inseminated. Ovary mapping and AI pregnancy rate were examined via transrectal ultrasonography. Blood samples were taken for genotyping: slick (S) or wildtype (W). Data were analyzed using repeated measures in PROC MIXED of SAS including fescue treatment (EN vs. EI), genotype (S vs. W), and sample collection time as main effects. Body condition scores were decreased for W heifers compared with S heifers (5.48 vs. 5.66, respectively; P < 0.0001). Surface temperature was greater for EI-W heifers (37.2 °C) compared with other groups (36.4, 36.6, 36.7 °C for EN-S, EN-W, EI-S, respectively; P < 0.05). Serum PRL concentrations were reduced for EI heifers compared with EN heifers (133.5 vs. 163.1 ng/mL, respectively; P < 0.05). The average number of 2 to 4 mm follicles were greater in EI-W heifers (13.8 follicles) compared with other groups (12.2, 10.6, and 11.1 for EN-S, EN-W, and EI-S, respectively; P < 0.0001). However, the average number of preovulatory follicles (≥9 mm) were reduced in EI-W heifers (0.52 follicles) compared with other heifer groups (0.94, 0.88, and 0.85 ± 0.04 for EN-S, EN-W, and EI-S, respectively; P < 0.05). Ovulatory follicle size was smaller in EI-W heifers compared with EN-W heifers (9.14 vs. 11.57 mm, respectively; P = 0.05). Corpus luteum area was reduced in EI-W heifers (235.1 mm2) compared with other heifer groups (297.2, 272.7, and 276.8 mm2 for EN-S, EN-W, and EI-S, respectively; P < 0.05). Concentrations of P4 were greater for EN heifers compared with EI heifers (2.7 vs. 1.8 ng/mL, respectively; P < 0.05). Pregnancy was not established in EI-W heifers (0%) compared with other heifer groups (37.5%, 57.1%, and 62.5% for EN-S, EN-W, and EI-S, respectively; P < 0.05). Overall, the slick hair mutation appears to aid in offsetting the physiological symptoms associated with fescue toxicosis and helps to improve reproductive performance.}, number={4}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poole, Rebecca K. and Devine, Thomas L. and Mayberry, Kyle J. and Eisemann, Joan H. and Poore, Matt H. and Long, Nathan M. and Poole, Daniel H.}, year={2019}, month={Apr}, pages={1456–1467} }
 @article{worthington_ott_poore_pickworth_2019, title={Impact of weaning strategy on beef cow performance.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.190}, abstractNote={Abstract
               It has been shown that physiologic stress in beef cattle can impact animal performance. Weaning stress is recognized as a major stress in a calf’s life. However, less is known of its impacts on the dam or developing fetus, as the dam is at the end of the second trimester at weaning. Therefore, the objective was to investigate the impact of weaning stress on cow performance. A total of 240 cows from two locations were assigned to one of three weaning strategies: abrupt removal of the calf on d 0 (n = 80), d 7 cow-calf fenceline contact (n = 81), and late weaning on d 84 (n = 79). Cows averaged 16 and 24 weeks of gestation on d 0 and d 84 respectively. Weights and BCS were obtained monthly. Behavioral observations occurred over the week before and after weaning for each weaning period. Five late-wean cows per location were milked on d 14 and d 70 to estimate milk yield throughout the late wean period. Data were analyzed using the MIXED procedure of SAS with BW and BCS utilizing repeated measures. Means are declared different at P ≤ 0.05. The ADG over the course of the study was lower (P < 0.05) for the late weaned cows compared to the abrupt weaned cows. This was also reflected by change in body condition where late weaned cows dropped from a 5.7 to a 4.9 (P < 0.05) over the 105 d. Milk production in late weaned cows decreased over time (6.0 and 4.4 kg/d for d 14 and 70, respectively; P = 0.05). Energy expenditure for lactation may have contributed to the drop in BCS of the late weaned compared with the non-lactating cows and could compromise third trimester growth of calf in utero. The results of this study warrant further exploration of the effect of weaning stress on dams and their developing fetuses.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Worthington, Mary Clarke and Ott, Grace C. and Poore, Matthew H. and Pickworth, Carrie L.}, year={2019}, month={Jul}, pages={83–83} }
 @article{ott_freeman_poore_pickworth_2019, title={Impact of weaning strategy on calf performance, behavior, and activity.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.152}, abstractNote={Abstract
               Weaning is known to be the most stressful time in a beef calf’s post-natal life. Weaning stress may have long-term impacts on cattle performance. This study was conducted to determine differences in calf stress under three weaning strategies. Two hundred twenty-nine Angus or Angus-Simmental cross pairs at two locations were assigned to one of three weaning treatments: abrupt removal of the calf on d 0 (Abrupt, n = 77), fenceline contact for 7 d prior to removal of the calf on d 7 (Fence, n = 79), and abrupt removal of the calf on d 84 (Late, n = 73). Behavioral observations were conducted pre- and post-weaning, and total activity was measured prior to and after weaning using accelerometers placed on collars on five calves per treatment. Calf weights, hip heights, and body condition scores (BCS) were collected to evaluate calf performance post-weaning. Data were analyzed using PROC MIXED of SAS. Statistical significance was determined at P < 0.05 and a tendency at 0.05 0.10) by timing or strategy of weaning. Total activity was greater (P < 0.05) in Abrupt than Fence on d 1 and d 2 post-wean (Abrupt 26772 and 22346 and Fence 22235 and 16691 relative activity units/hour, respectively). Calf activity was elevated (P < 0.05) on day of weaning until d 2 post-wean then returned to pre-wean level by d 3 for all treatments. Short-term differences in calf performance warrant further exploration of the possible long-term effects on heifer development and steer feedlot performance.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Ott, Grace C. and Freeman, Sharon R. and Poore, Matthew H. and Pickworth, Carrie L.}, year={2019}, month={Jul}, pages={67–68} }
 @article{davis_poole_poore_poole_pickworth_2019, title={Interaction of dietary protein and endophyte-infected Tall Fescue seed in stocker steers on finishing phase performance and carcass traits.}, volume={97}, ISBN={1525-3163}, DOI={10.1093/jas/skz053.086}, abstractNote={Abstract
               Fescue toxicosis is a multifaceted disease that negatively impacts cattle. It was hypothesized that exposure to endophyte-infected fescue during the stocker phase affects finishing phase growth and carcass characteristics. Eight-month-old steers were sorted by weight and randomly assigned to receive one of four treatments for 56 days in confinement: endophyte-free seed (0 ug/ kg ergovaline) with either 18% (EF-18; n = 9) or 14% dietary protein (EF-14; n = 9) and endophyte-infected seed (500 ug/kg ergovaline) with 18% (EI-18; n = 9) or 14% dietary protein (EI-14; n = 9). Following fescue exposure calves were backgrounded on novel endophyte fescue pastures for 8 mo. At 18 mo, all steers were vaccinated, implanted, and reentered the feedlot for a 112 d growing phase and 52 d finishing phase. Body weight was recorded every 28 d and carcass data was collected at slaughter. Data were analyzed using the MIXED procedure of SAS for effects of BW, ADG, dressing percentage (DP), HCW, backfat, ribeye area (REA), KPH, USDA Yield grade (YG), and USDA Quality grade (QG). Statistical significance was determined at P > 0.05) HCW, BW, backfat, REA, DP, or QG. TheEI-14 had a greater ADG (1.69 kg/d) during the feedlot growing phase compared to the other treatment groups (1.53, 1.47, 1.55 for EI-18, EF-14, EF-18, respectively; P > 0.05). Steers stockered on 18% dietary protein had greater BW compared to steers previously fed at 14% (501.3±3.8 vs. 489.7±3.8; P = 0.0339). In addition, 18% protein in stocker diet resulted in greater KPH (2.3±0.2 vs. 1.78±0.2; P = 0.0129) and YG (3.4±0.1 vs. 3.0±0.1; P = 0.0168) compared with steers fed 14% dietary protein. Finishing growth and carcass characteristics were not affected by exposure to ergot alkaloids at weaning as much as dietary protein when cattle had a background period prior to feedlot entry}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Davis, Matthew E. and Poole, Rebecca K. and Poore, Matthew H. and Poole, Daniel H. and Pickworth, Carrie L.}, year={2019}, month={Jul}, pages={38–38} }
 @article{jackson_newsome_brown_poole_dudley_poore_poole_2019, title={Long term impact of ergot alkaloids on fetal heifer development and subsequent growth and reproductive performance.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.103}, abstractNote={Abstract
               Previous studies from our laboratory have demonstrated physiological differences in mid-gestation cows grazing either endophyte-infected (EI) or endophyte-free (EF) tall fescue. The objective of this study was to investigate the effects of in utero exposure (TRT) of either EI or EF on heifer growth, reproductive tract development and onset of puberty. Pregnant Angus and SimAngus cows were blocked by age, BW, and breed; and then randomly assigned to graze either EF (n = 27) or EI (n = 27) pastures. At parturition, heifer birthweight was recorded and placental tissues were collected to assess placental characteristics. Heifer BW, height, frame score were recorded at weaning. Assessment of puberty via measurement of BW, BCS, reproductive tract scores (RTS) and pelvic area (PA) started at 10 mo of age. Data were analyzed using a MIXED procedure of SAS and examined for effects of TRT, sire, and age. Statistical significance was determined at P < 0.05 and a tendency at 0.05); however, there was a sire by treatment interaction for ADG during onset of puberty (P < 0.05). While TRT did not affect PA or RTS (P > 0.05) at 10 mo of age, sire significantly influenced RTS (P < 0.05) thus impacting of onset of puberty. While consumption of EI fescue impacts cow performance, calf birthweight, and ratio of cotyledon to placenta weight, it has minimal effect on postnatal growth and development through 10 mo of age. Further studies are needed to determine the impact on heifer reproductive performance.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Jackson, McKenzie Lane and Newsome, McKayla A. and Brown, Alecia R. and Poole, Rebecca K. and Dudley, Harrison B. and Poore, Matthew H. and Poole, Daniel H.}, year={2019}, month={Jul}, pages={46–46} }
 @article{rogers_poore_ingram_harmon_2019, title={On-farm demonstrations improve producer understanding of using metsulfuron for tall fescue seedhead suppression.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.011}, abstractNote={Abstract
               In 2018, a series of 4 on-farm demonstrations were conducted using a metsulfuron herbicide for tall fescue (Lolium arundinaceum) seedhead suppression to increase producer awareness of this practice. Chaparral© herbicide was applied prior to the boot stage (April 5–April 21, 2018) and the design was an unreplicated field with side by side treated and untreated areas. Forage samples were taken to determine yield, nutritive value, species composition and ergovaline level. Treatment with Chaparral© increased crude protein (12.1 vs. 14.2%) while reducing yield (5477 vs. 3738 kg/ha) and tall fescue seedhead proportion (42.8 vs. 25.4%) (P < 0.05). Total digestible nutrients tended to be higher (57.2 vs 59.2%, P = 0.08) and ergovaline level tended to be lower (901 vs. 491 ppb, P = 0.11) with treatment. Workshops were held to report the demonstration results. Total workshop registration was 147 with 91 evaluations completed. Participants were 78% male and 22% female with 65% being over the age 50. The pasture hectares grazed by participants was 4253 with 1415 ha as hay. Attendee’s livestock inventories were 6357 beef cattle, 105 sheep, 66 goats and 70 horses. Post-workshop evaluation indicated that 100% of attendees increased understanding of fescue toxicosis, management strategies to reduce fescue toxicity and of using metsulfuron for seedhead suppression. Evaluations completed at the conclusion of each workshop (n = 90) indicated satisfaction with (scale of 1 = not satisfied to 4 = very satisfied, mean ± SD): instructors’ knowledge (3.86 ± 0.34), workshop environment/format (3.83 ± 0.37), and overall quality (3.82 ± 0.41). One hundred percent indicated that this workshop met their expectations and they would recommend it to others. Producer interest and responses indicate that on farm demonstrations are an effective method for education of new production practices}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Rogers, Johnny R. and Poore, Matthew H. and Ingram, Sam and Harmon, Deidre}, year={2019}, month={Jul}, pages={5–6} }
 @article{poore_scaglia_andrae_harmon_rogers_blacklin_2020, title={Pasture-finished beef production in the south}, ISBN={["978-0-12-814474-9"]}, DOI={10.1016/B978-0-12-814474-9.00010-4}, abstractNote={There has been increased interest in pasture-based systems for finishing beef cattle in the Southern United States. These systems include grass-fed, pasture-finished, and many other variations. The local food movement has provided farmers with new outlets through farmers' markets, restaurants, and independent grocery stores, and various business models are being explored to deliver consistent and high-quality products to consumers. Farmers need to define and document their production system to support any production claims they make on their beef packaging labels or marketing materials. Farmers developing these systems must overcome many challenges with economy of scale, unsteady forage supply and nutritive value, lack of processing infrastructure, and consumer misconceptions about animal welfare, human health benefits, and environmental impacts. There are compositional differences between conventional feedlot beef and pasture-raised beef, including differences in total fat, fatty acid composition, and vitamin levels. However, because beef is only a small part of the overall diet, these differences have limited implications for human health. Forage system research has identified some of the weaknesses of Southern pasture–based beef finishing systems, but inflexibility in study design makes it difficult to mimic the systems used by producers. Most producers are targeting a significant level of marbling in meat, so that they either supplement concentrates on forage-based diets, use high-quality forages, and/or keep animals to older ages than may be typical for conventional feedlot beef. Regardless of the production system, providing animals with high-quality grazed or harvested forages as much of the year as possible will improve meat quality and improve the sustainability of these systems.}, journal={MANAGEMENT STRATEGIES FOR SUSTAINABLE CATTLE PRODUCTION IN SOUTHERN PASTURES}, author={Poore, Matt and Scaglia, Guillermo and Andrae, John and Harmon, Deidre and Rogers, Johnny and Blacklin, Sarah}, year={2020}, pages={265–299} }
 @article{ingram_poore_poole_2019, title={Renovating toxic-infected tall fescue pastures for profitability, animal performance, and soil health.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.084}, abstractNote={Abstract
               Renovating toxic-infected tall fescue (TF) pastures to non-toxic infected tall fescue (NE) for profitability, animal and agronomic performance without compromising soil health is needed to transition to NE in the southeast U.S. Three renovation strategies were evaluated for impact on soil health, profitability, and animal and agronomic performance in a replicated field trial located in Bahama, NC. Strategies included: 1) control (C), 2) renovation to NE after one season of a single specie cover crop (1-SM), 3) renovation to NE after three seasons of a single specie cover crop (3-SM), and 4) renovation to NE after three seasons of a multi-specie cover crop (3-CM). Soil samples were taken prior to renovation to establish baseline soil health measurements for each strategy. Data were analyzed using proc GLM of SAS v9.4. Thirty-two Angus and Angus cross steers (initial BW 425 ± 27) were blocked by BW and randomly assigned to one of four treatments during the first season of renovation. Average daily gain for cattle grazing the first season of cover crops was greatest (P < 0.001) with 3-CM (0.8 kg/d), 3-SM (0.8 kg/d), in comparison to C and 1-SM (-0.1 kg/d). Gain per hectare differed among treatments (P < 0.001) with 3-SM and 3-CM being greater than C and 1-SM (179, 154, -24, -43 kg/ha, respectively). Within the two-month grazing period, yield different during the month of August (P < 0.001), with 3-SM being the highest (4511 kg/ha) in comparison to 3-CM (3275 kg/ha), 1-SM (2922 kg/ha), and C (2644 kg/ha), while yield was highest (P < 0.001) for 3-CM in September (2931 kg/ha) in comparison to all other treatments. Data suggests agronomic and animal performance for 3-CM and 3-SM were improved compared to C and could partially alleviate initial renovation costs.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Ingram, Sam and Poore, Matthew H. and Poole, Daniel H.}, year={2019}, month={Jul}, pages={37–38} }
 @article{ingram_poore_poole_2019, title={Renovating toxic-infected tall fescue pastures for profitability, animal performance, and soil health.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.044}, abstractNote={Abstract
               Renovating toxic-infected tall fescue (TF) pastures to non-toxic infected tall fescue (NE) for profitability, animal and agronomic performance without compromising soil health is needed to transition to NE in the southeast U.S. Three renovation strategies were evaluated for impact on soil health, profitability, and animal and agronomic performance in a replicated field trial located in Bahama, NC. Strategies included: 1) control (C), 2) renovation to NE after one season of a single specie cover crop (1-SM), 3) renovation to NE after three seasons of a single specie cover crop (3-SM), and 4) renovation to NE after three seasons of a multi-specie cover crop (3-CM). Soil samples were taken prior to renovation to establish baseline soil health measurements for each strategy. Data were analyzed using proc GLM of SAS v9.4. Thirty-two Angus and Angus cross steers (initial BW 425 ± 27) were blocked by BW and randomly assigned to one of four treatments during the first season of renovation. Average daily gain for cattle grazing the first season of cover crops was greatest (P < 0.001) with 3-CM (0.8 kg/d), 3-SM (0.8 kg/d), in comparison to C and 1-SM (-0.1 kg/d). Gain per hectare differed among treatments (P < 0.001) with 3-SM and 3-CM being greater than C and 1-SM (179, 154, -24, -43 kg/ha, respectively). Within the two-month grazing period, yield different during the month of August (P < 0.001), with 3-SM being the highest (4511 kg/ha) in comparison to 3-CM (3275 kg/ha), 1-SM (2922 kg/ha), and C (2644 kg/ha), while yield was highest (P <0.001) for 3-CM in September (2931 kg/ha) in comparison to all other treatments. Data suggest agronomic and animal performance for 3-CM and 3-SM were improved compared to C and could partially alleviate initial renovation costs.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Ingram, Sam and Poore, Matthew H. and Poole, Daniel H.}, year={2019}, month={Jul}, pages={20–20} }
 @article{franzluebbers_poore_freeman_rogers_2019, title={Soil-surface nutrient distributions in grazed pastures of North Carolina}, volume={74}, ISSN={["1941-3300"]}, DOI={10.2489/jswc.74.6.571}, abstractNote={Soil nutrient distribution in perennial pastures is likely affected by livestock activities, but detailed spatial patterns on a diversity of farms have not been widely investigated. Livestock management variables, such as placement of drinking water sources, fencing, and winter hay-feeding stations, as well as pasture utilization through seasonal stocking, could affect nutrient distribution despite attempts at uniform application of fertilizers. We sampled perennial pasture portions of three research station farms in North Carolina on a 40 m grid in 2016. Twenty-two soil variables were measured, including routine soil testing of pH, cation exchange capacity, and extractable phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg), as well as nonroutine testing of sand concentration, total and particulate organic carbon (C) and nitrogen (N), and soil-test biological activity. Large statistical variations in nearly all variables occurred within a location. Severely deficient and heavily excessive levels of soil-test P and K were observed on the same farm and sometimes on the same paddock. Soil-test biological activity and total soil N also varied spatially within locations. High nutrient concentrations and soil-test biological activity were congregated in zones of several pastures, which could be related to historical winter hay-feeding and permanent drinking water locations. At the Piedmont location with recent winter hay feeding, both organic and inorganic indicators generally corresponded to the same enrichment zones. At the Coastal Plain location with abandonment of some historical feeding locations, soil-test P remained high near feeding stations, but soil-test K and total soil N became more uniformly distributed due to leaching and/or decomposition dynamics over time. We suggest that both organic and inorganic soil testing indicators should be measured to make nutrient recommendations for better resource utilization. Livestock managers are encouraged to sample pastures in expected zones of nutrient differentiation so that nutrients can be used efficiently and environmental threats can be mitigated.}, number={6}, journal={JOURNAL OF SOIL AND WATER CONSERVATION}, author={Franzluebbers, A. J. and Poore, M. H. and Freeman, S. R. and Rogers, J. R.}, year={2019}, pages={571–583} }
 @article{poore_stratton_roberts_smith_andrae_pent_shaeffer_2019, title={The Alliance for Grassland Renewal presents workshops to enhance the understanding and adoption of Novel Endophyte Tall Fescue.}, volume={97}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skz053.018}, abstractNote={Abstract
               The Alliance for Grassland Renewal (the Alliance) is a collaborative multi-state effort to enhance the understanding of fescue toxicosis and the adoption of Novel Endophyte Tall Fescue (NETF). The Alliance includes academic institutions, companies marketing NETF, allied companies, governmental agencies, and non-profits. The Alliance initially focused on the state of Missouri. After 2014, it expanded efforts with one-day workshops in MO, KY, OK, KS and UKY joined the board in 2017. In 2018, Clemson and NCSU joined the board, and workshops were expanded to the east coast, with five workshops in MO, KY, SC, NC and VA. In 2019, workshops will repeated in those states in addition GA. Key presentations included managing fescue toxicosis, establishment and first year management, second year management, and economics of conversion. Additionally the agenda included microscope viewing of the endophyte, quality control and endophyte testing, industry update, drill calibration, producer panel, and a tour of NETF plots. There were 214 paid attendees in 2018 (locations ranged 26 to 59; 75% farmers), and 146 evaluations were submitted. Attendees owned 9592 beef or dairy cows, 1997 stocker cattle, 121 horses, 545 sheep and goats, and 151 alpacas. Thirty percent of the audience had planted NETF in the past, while 55% intended to plant NETF in the future. Reasons given for not planting included: unclear cost/benefit (9%), limited financial resources (4%), lack of knowledge (5%), rented or unsuitable land (15%) and forage reserves lacking (5%). Registration fee was $60, and 45% of the audience said they would have paid $95, suggesting the registration fee was appropriate. The Alliance is a growing and successful partnership between academic institutions and industry. This approach is a model that could be applied to adoption of other new technologies.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poore, Matthew H. and Stratton, Tony and Roberts, Craig A. and Smith, S. Ray. and Andrae, John G. and Pent, Gabe J. and Shaeffer, April D.}, year={2019}, month={Jul}, pages={8–9} }
 @article{ott_freeman_poore_pickworth_2018, title={27 Maternal Stress at Weaning Impacts Calf Growth.}, volume={96}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/sky027.028}, DOI={10.1093/jas/sky027.028}, number={suppl_1}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Ott, G C and Freeman, S R and Poore, M H and Pickworth, C L}, year={2018}, month={Mar}, pages={14–15} }
 @article{ingram_poore_2018, title={Comparison of a Full Season and Early Maturing Forage Sorghum Silage to Corn Silage for Growing Cattle.}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/sky027.058}, abstractNote={Brachytic dwarf brown mid-rib forage sorghum varieties are an alternative to corn silage (CS) in certain production systems. Agronomic characteristics, cattle performance and carcass data were collected during this project. Full season forage sorghum (FS,110–115 days), early maturing forage sorghum (ES, 85–89 days), and a full season CS were grown and harvested in a replicated field trial in Bahama, NC. Overall mean yield (kg DM/ha) did not differ (P < 0.12; 6772 kg/ha). Silages contained 30.9, 27.5, 28.3% DM for CS, FS and ES, 68.5, 63.5, 64.7 TDN (% of DM); and 9.6, 8.2, 8.7% CP, respectively. Sixty-six Angus and Angus cross steers (initial BW 329 ± 35 kg) were penned by BW and randomly assigned to one of three treatments. Steers were individually fed ad libitum utilizing Calan gates for a 77-d growing period and a 88-day finishing period. Diets were formulated to 14% CP and were 75% CS, FS or ES and 25% concentrate (DM basis) during growing and 15% CS and 85% concentrate (DM basis) during finishing. Cattle were harvested in Pennsylvania and carcass data were collected. During the growing period, DMI and ADG for the growing period differed among treatments (P < 0.05; P < 0.01) with intake and gain being greatest (9.85 kg/d; 1.95 kg/d) for CS with no difference between FS (8.51kg/d; 1.46 kg/d) and ES (8.69 kg/d; 1.50 kg/d). During finishing, ADG was higher (P < 0.02) for FS (1.80 kg) and ES (1.86 kg) compared to CS (1.66 kg) while finishing DMI did not differ (P < 0.78; 12.10 kg). Feed:gain during the growing period differed (P < 0.01) with CS (4.83) being more efficient than FS (5.90) and ES (6.02). Feed:gain during finishing was lower (P < 0.05) for FS (6.80) and ES (6.65) compared to CS (7.27). Loin eye area did not differ (P < 0.41; 90.3 cm2), but quality grade (17 = low choice and 18 = mid choice) differed (P < 0.03) between CS (18.24) and ES (17.39), but not between CS and FS (17.83, P < 0.17). Brachytic dwarf brown mid-rib forage sorghum silages limited animal performance during the growing period but steers compensated during the finishing period.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Ingram, S. and Poore, M.}, year={2018}, month={Mar}, pages={31–31} }
 @article{freeman_lahti_poore_2018, title={Creating Partnerships with the US Military.}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/sky027.018}, abstractNote={The US Army reached out to NC Cooperative Extension Service (NCCES) for assistance in training soldiers working with indigenous farmers while performing their duties during deployments worldwide. Two, independent training opportunities were developed. Basic Animal Husbandry (BAH) training began in 2011 and has grown from an annual, one-day course to a four-day, biannual training offered to Army Medics from Fort Bragg. It has served over 75 personnel from 2 Battalions and, in one year, included Navy Corpsmen from Camp Lejeune. Training session topics are digestive and reproductive physiology; nutrition; handling common livestock species; biosecurity/zoonosis; cost effective solutions to common health problems; and global perspective on the tie between animal and human health and welfare. The Rural Engagement and Mapping Course (REMC) began in 2015 as a one week agricultural immersion course. Its focus is increasing basic agricultural knowledge through exposure to agriculture, smallholder farms, and farm culture. It provides real-world experience through engaging with farmers and developing solutions to real problems as well as fostering self-awareness and personal development. To date, 106 soldiers from 4 different battalions have completed the training. When NCCES realized there would be ongoing demand for both courses, the desire for feedback from participants to enable ongoing improvement in the courses increased. Trainers began asking soldiers to complete self-assessment evaluations to determine change in knowledge base and to suggest areas in need of change or improvement. Mean before and after scores (Table 1; n = 51 for BAH and 18 for REMC) indicate success. Continued demand for both courses also indicates the Army values the products NCCES has developed. Both trainings are made possible through partnerships. Cooperative Extension partners with local farmers for REMC, while BAH partners NCCES staff from the Animal Science Department and College of Veterinary Medicine at NC State University. The success of these programs has had global impact and is a testament to the value of partnerships. Table 1. Training results for Civil Affairs Battalions self-assessments 1Scale 0 to 4: 0 = no knowledge, 4 = complete understanding 2Scale 1 to 10: 1 = no knowledge, 10 = complete understanding 3Scale 0 to 15: pre-/post-test scores out of 15; not paired by individual Table 1. Training results for Civil Affairs Battalions self-assessments 1Scale 0 to 4: 0 = no knowledge, 4 = complete understanding 2Scale 1 to 10: 1 = no knowledge, 10 = complete understanding 3Scale 0 to 15: pre-/post-test scores out of 15; not paired by individual}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Freeman, S. R. and Lahti, L. J. and Poore, M.}, year={2018}, month={Mar}, pages={9–10} }
 @article{silva_poore_ranches_santos_moriel_2018, title={Effects of gradual reduction in frequency of energy supplementation on growth and immunity of beef steers}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skx047}, abstractNote={This study evaluated the effects of gradual reduction in frequency of energy supplementation following vaccination on growth and measurements of innate and humoral immunity of beef steers. At 14-d postweaning (d 0), Angus steers (n = 42; 200 ± 5 kg of BW; 175 ± 4 d of age) were stratified by BW and age, and randomly assigned into 1 of 14 drylot pens (three steers/pen). From d 0 to 42, steers were provided ad libitum ground tall fescue hay (57% TDN, 13% CP of DM basis) and supplemented with concentrate at 1% of BW (50:50 soybean hulls and corn gluten feed; 71% TDN, 15% CP of DM basis). Treatments were randomly assigned to pens, and consisted of similar weekly concentrate DM supplementation (1% of BW multiplied by 7 d) that was divided and offered daily from d 0 to 42 (7X; 4 pens), 3 times weekly from d 0 to 42 (3X; Monday, Wednesday, and Friday; 5 pens), or daily from d 0 to 15 and then 3 times weekly from d 16 to 42 (7-3X; 5 pens). Steers were vaccinated against infectious bovine rhinotracheitis (IBR), bovine viral diarrhea virus (BVDV), parainfluenza-3 (PI-3), Mannheimia haemolytica, and Clostridium on d 0 and 15. Individual shrunk BW was collected on d 0 and 42, following 12 h of feed and water withdrawal. Blood samples were collected via jugular venipuncture 4 h after concentrate supplementation on d 0, 1, 2, 3, 7, 15, 16, 17, 18, 22, and 42. Mean BW, ADG, G:F, hay DMI, and total DMI over the 42-d period did not differ among treatments (P ≥ 0.26). Plasma concentrations of cortisol and mean serum BVDV-1a titers also did not differ among treatments (P ≥ 0.35), but overall plasma haptoglobin concentrations were greater for 3X vs. 7-3X and 7X steers (P ≤ 0.05; 0.44, 0.37, and 0.33 ± 0.026 mg/mL, respectively). Also, 3X steers had less mean serum IBR titers (P ≤ 0.05; 0.29 vs. 0.88 and 0.79 ± 0.179 log2, respectively) and less seroconversion to PI-3 virus on d 15 than 7-3X and 7X steers (P ≤ 0.05; 36.0 vs. 76.6 and 57.8 ± 8.24%, respectively). In summary, a gradual reduction in frequency of energy supplementation during a 42-d preconditioning period did not negatively impact growth, but alleviated indices of inflammation and prevented reductions in vaccine response against BVDV-1a and PI-3 viruses compared to steers offered concentrate 3 times weekly during the entire study.}, number={1}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Silva, Gleise M. and Poore, Matt H. and Ranches, Juliana and Santos, Glauber S. and Moriel, Philipe}, year={2018}, month={Jan}, pages={273–283} }
 @article{silva_poore_ranches_moriel_2018, title={Effects of timing of vaccination relative to weaning and post-weaning frequency of energy supplementation on growth and immunity of beef calves}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skx021}, abstractNote={A 2 × 2 factorial design study evaluated the impact of pre- vs. post-weaning vaccination and different post-weaning frequency of energy supplementation (daily vs. 3X weekly) on growth and immunity of beef calves. At 14 d before weaning (d -14), 48 Angus calves (24 steers and 24 heifers; 244 ± 33 kg; 196 ± 20 d) were stratified by BW and age, and randomly assigned to receive vaccinations against bovine viral diarrhea virus 1a (BVDV-1a) and parainfluenza-3 (PI-3) on d -14 and 0 (PRE) or 7 and 21 (POS), relative to weaning. Calves were weaned on d 0 and offered daily concentrate DM supplementation (50:50 soybean hulls and corn gluten feed; 71% TDN, 15% CP of DM) at 0.5% of BW for 7 d. On d 7, calves were stratified by vaccination scheme and assigned into 1 of 16 drylot pens (3 calves of same sex/pen; 4 pens/treatment). Pens were randomly assigned to receive similar weekly concentrate DM supplementation (1% of BW multiplied by 7 d) that was divided and offered daily (7X) or three times weekly (3X; Mondays, Wednesdays, and Fridays) from d 7 to 43. From d 0 to 43, calves were provided ad libitum ground tall fescue hay (57% TDN, 13% CP of DM). Blood samples were collected from jugular vein on d 0, 1, 3, 7, and 14, relative to the respective first vaccination, and on d 43 of the study. Effects of timing of vaccination × frequency of supplementation were not detected for any variable in this study (P ≥ 0.12), except for overall ADG from d -14 to 43 (P = 0.04), which was less for PRE-3X vs. PRE-7X, POS-3X, and POS-7X calves (0.60, 0.70, 0.70, and 0.77 ± 0.04 kg/d, respectively; P ≤ 0.08). Post-weaning total DMI and G:F did not differ among treatments (P ≥ 0.11). Pre-weaning vaccination increased plasma concentrations of cortisol and haptoglobin from d 0 to 3, relative to first vaccination (P ≤ 0.03), and decreased serum PI-3 titers on d 43 compared with post-weaning vaccination (P < 0.0001). Decreasing the supplementation frequency tended (P = 0.10) to increase overall plasma cortisol concentrations and reduce overall serum BVDV-1a titers. Hence, pre-weaning vaccination associated with reduced post-weaning frequency of energy supplementation caused the greatest reduction on calf growth performance. Post-weaning vaccination and daily energy supplementation alleviated inflammation and improved humoral immunity compared with pre-weaning vaccination and reduced post-weaning frequency of energy supplementation of recently weaned beef calves.}, number={1}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Silva, Gleise M. and Poore, Matthew H. and Ranches, Juliana and Moriel, Philipe}, year={2018}, month={Jan}, pages={318–330} }
 @article{poole_lyons_poole_poore_2018, title={Ergot alkaloids induce vasoconstriction of bovine uterine and ovarian blood vessels}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/sky328}, abstractNote={Fescue toxicosis is a syndrome that impairs growth and reproduction in cattle grazing endophyte-infected tall fescue [Lolium arundinaceum [(Schreb.].) Darbysh)] in the United States, resulting in approximately $1 billion in annual economic loss in species that utilize this forage resource. Approximately 90% of tall fescue contains an endophytic fungus (Epichloë coenophiala) that produces ergot alkaloids. Ergot alkaloids cause vasoconstriction and reduced blood flow to the extremities; however, it remains unknown how blood flow to the reproductive organs is affected in cattle. Therefore, the objective of this study was to determine if ergot alkaloids from endophyte-infected tall fescue reduce blood flow to the reproductive organs, thus hindering reproductive function. Angus heifers (n = 36) naïve to ergot alkaloids were placed in Calan gates and randomly assigned to receive either endophyte-infected fescue seed (E+) or noninfected fescue seed (E-; control) in a total mixed ration for 63 d. Weekly measurements were taken to monitor heifer growth and response to ergot alkaloid exposure. Reproductive measurements, including ovarian structures, uterine and ovarian vessel diameter, and hormone concentrations were determined after heifers were synchronized using the standard CO-Synch + 7 d CIDR protocol to ensure all measurements were collected at the same stages of the estrous cycle (0, 4, 10, and 17 d). Data were analyzed using repeated measures in PROC MIXED of SAS. Average daily gain was decreased for the E+ group (0.8 kg/d) compared to control heifers (1.0 kg/d). Body condition scores tended to be greater in control heifers compared to the E+ group (P = 0.053). Additionally, hair coat and hair shedding scores were greater in E+ heifers compared to controls (P < 0.05). Heart rate, rectal temperature, respiration rate, and blood pressure did not differ between treatments (P > 0.05). Vasoconstriction was observed in the caudal artery, but not the caudal vein, in heifers consuming the E+ fescue seed (P < 0.05). No differences were observed in antral follicle counts, corpus luteum area or circulating progesterone concentrations in E+ heifers compared to controls (P > 0.05). There was a significant decrease in the diameter of arteries and veins servicing the ovary and uterus on day 10 and 17 of the estrous cycle. Reduction in blood flow to the reproductive organs during critical times in the estrous cycle may contribute to the reduced ovarian function and pregnancy rates associated with fescue toxicosis.}, number={11}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poole, Daniel H. and Lyons, Sarah E. and Poole, Rebecca K. and Poore, Matt H.}, year={2018}, month={Nov}, pages={4812–4822} }
 @article{mayberry_khanal_poore_serao_poole_2018, title={Evaluation of Angus Calf Performance Based on Dams Tolerance or Susceptibility to Fescue Toxicosis.}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/sky027.103}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Mayberry, K. and Khanal, P. and Poore, M. H. and Serao, N. V. L. and Poole, D. H.}, year={2018}, month={Mar}, pages={55–55} }
 @article{newsome_monn_mayberry_poole_young_poore_poole_2018, title={Grazing Novel or Endophyte-Infected Tall Fescue during Mid-Gestation Impacts Cow Performance.}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/sky027.025}, abstractNote={Consumption of endophyte-infected tall fescue results in poor reproductive performance. The objective of this study was to examine physiological changes in pregnant cows grazing tall fescue to develop a better understanding of the factors that contribute to the poor reproductive performance attributed to fescue toxicosis. Pregnant Angus and Simmental-Angus cows were blocked by age (2–3, 4–7, and >7 y), BW, and breed; and then randomly assigned to graze either novel (E-; <5% infection rate; n=27) or endophyte-infected tall fescue (E+; 79.5% infection rate; n=27). Weekly BW, BCS, hair coat scores (HCS), and hair shedding scores (HSS) were collected from mid-April through July when ergot alkaloid concentrations are greatest in the forage. Blood samples were collected to measure progesterone concentrations. Forage samples were taken every 2 wks to determine forage quality and composition. Data were analyzed using the MIXED procedure of SAS with repeated measures examined for effects of age, treatment (E- or E+) and time. Statistical significance was determined at P<0.05 and a tendency at 0.05<P<0.10. Pastures consisted of 69% and 67.5% available fescue for the novel and endophyte infected pastures, respectively. Average daily gain tended to be higher in E- cows than E+ (0.16, 0.01 kg/d, respectively; P=0.09). Cows on E- pastures had greater BCS compared to cows on E+ pastures (6.09, 5.77, respectively; P<0.05). Similarly, an age effect was observed with mature (4–7 and >7 y) cows having higher scores when compared to younger cows (6.0, 6.1, and 5.7, respectively; P<0.05). The HCS and HSS were greater in cows on E+ pastures compared to cows on E- pastures (P<0.05). The HSS were greater in the >7 y cows (2.69) compared to other age groups (2.40, 2.51, for 2–3 and 4–7 y, respectively, P<0.05) on E+ pastures, whereas no age differences in HCS were observed in cows on E- pastures. By d21 of the grazing period, cows on E- pastures displayed a greater ability to shed hair compared to cattle on E+ pastures (P<0.0001), which continued throughout the remainder of the grazing period. Progesterone concentrations in E+ cows (3.75 ng/ml) were decreased throughout the grazing period compared to E- cows (6.53 ng/mL, P<0.05). The impact of reduced progesterone concentrations, greater HCS and HSS and tendency for decreased ADG in cows grazing E+ during mid-gestation, compared to cows grazing E-, on calf development remains unknown.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Newsome, M. A. and Monn, R. E. and Mayberry, K. and Poole, R. K. and Young, Z. and Poore, M. H. and Poole, D. H.}, year={2018}, month={Mar}, pages={13–13} }
 @article{poore_shaeffer_rogers_freeman_2018, title={Hands-on Workshops Spark Producer Interest in Improving Mineral Programs for Beef Cattle.}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/sky027.007}, abstractNote={Mineral supplements are a key input for grazing beef cattle, and the wide variety of products and management approaches is confusing to producers. To address basic mineral requirements and mineral program management, four Amazing Grazing workshops were conducted across North Carolina with funding support from the NC Cattle Industry Assessment Program. Workshops were conducted in collaboration with county livestock extension agents who managed the meeting facilities and logistics, and provided tires and barrels for mineral feeder construction. A state specialist provided the educational materials and also the hardware and tools needed for mineral feeder construction. The presentations were informal and included 1) the basics of mineral requirements and typical forage mineral composition, 2) how to read and interpret a mineral tag, 3) how to determine a need for added mineral technologies such as “organic or chelated” trace minerals, ionophores, weather proofing, etc, and 4) management strategies including how to choose a good mineral feeder. As a hands-on activity producers worked together to build simple tire and barrel mineral feeders which they took home with them from the workshop. Enough supplies were on hand such that each participating farm could take a mineral feeder home if they wished. At the end of the workshop participants filled out a survey to represent each farm participating. Total workshop attendance was 86 (mean and standard deviation; 22 ± 7) and 57 (14 ± 6) surveys were collected. Workshop participants managed 2690 cows, 971 yearlings, and 4532 acres of pasture. All participants were satisfied or very satisfied with the workshop overall, and said they would recommend the workshop to other producers. A total of 49 mineral feeders were built (12 ± 3), and participants indicated they intended to go home and build an additional 81 feeders. Ninety % of the participants indicated they intended to use the information presented to improve their mineral program. Economic impact was determined by assuming that improving a mineral program would improve net income per animal by $10, and mineral feeders were valued at $150. Total economic impact was $52,499. Of all comments about what participants liked most about the workshop, “the hands-on activity” was most common. This program has been adapted for local use by a number of extension agents, and an accompanying YouTube video and set of plans for constructing these mineral feeders have been valuable outreach tools.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poore, M. H. and Shaeffer, A. D. and Rogers, J. R. and Freeman, S. R.}, year={2018}, month={Mar}, pages={4–4} }
 @article{sanglard_nascimento_moriel_sommer_ashwell_poore_duarte_serão_2018, title={Impact of energy restriction during late gestation on the muscle and blood transcriptome of beef calves after preconditioning}, volume={19}, ISSN={1471-2164}, url={http://dx.doi.org/10.1186/s12864-018-5089-8}, DOI={10.1186/s12864-018-5089-8}, abstractNote={Maternal nutrition has been highlighted as one of the main factors affecting intra-uterine environment. The increase in nutritional requirements by beef cows during late gestation can cause nutritional deficiency in the fetus and impact the fetal regulation of genes associated with myogenesis and immune response.Forty days before the expected calving date, cows were assigned to one of two diets: 100% (control) or 70% (restricted group) of the daily energy requirement. Muscle samples were collected from 12 heifers and 12 steers, and blood samples were collected from 12 steers. The objective of this work was to identify and to assess the biological relevance of differentially expressed genes (DEG) in the skeletal muscle and blood of beef calves born from cows that experienced [or not] a 30% energy restriction during the last 40 days of gestation.A total of 160, 164, and 346 DEG (q-value< 0.05) were identified in the skeletal muscle for the effects of diet, sex, and diet-by-sex interaction, respectively. For blood, 452, 1392, and 155 DEG were identified for the effects of diet, time, and diet-by-time interaction, respectively. For skeletal muscle, results based on diet identified genes involved in muscle metabolism. In muscle, from the 10 most DEG down-regulated in the energy-restricted group (REST), we identified 5 genes associated with muscle metabolism and development: SLCO3A1, ATP6V0D1, SLC2A1, GPC4, and RASD2. In blood, among the 10 most DEG, we found genes related to response to stress up-regulated in the REST after weaning, such as SOD3 and INO80D, and to immune response down-regulated in the REST after vaccination, such as OASL, KLRF1, and LOC104968634.In conclusion, maternal energy restriction during late gestation may limit the expression of genes in the muscle and increase expression in the blood of calves. In addition, enrichment analysis showed that a short-term maternal energy restriction during pregnancy affects the expression of genes related to energy metabolism and muscle contraction, and immunity and stress response in the blood. Therefore, alterations in the intra-uterine environment can modify prenatal development with lasting consequences to adult life.}, number={1}, journal={BMC Genomics}, publisher={Springer Science and Business Media LLC}, author={Sanglard, Leticia P and Nascimento, Moysés and Moriel, Philipe and Sommer, Jeffrey and Ashwell, Melissa and Poore, Matthew H and Duarte, Márcio de S and Serão, Nick V L}, year={2018}, month={Sep} }
 @article{monn_shea_mayberry_freeman_poole_poore_2018, title={Implementing Shade and Slick Hair Phenotypes Improves Heifer Performance}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/sky027.150}, abstractNote={Studies have shown that proper regulation of body temperature is essential for optimum productivity in cattle. The objective of this pilot study was to determine if the shade provided by silvopasture or incorporation of the slick hair phenotype improves beef heifer performance. Senepol cross beef heifers (n=12) were blocked by hair coat phenotype, either slick or normal, and randomly assigned to graze shaded or non-shaded pastures from May through October. During this grazing period, BCS, hair coat scores (HCS), hair shedding scores (HSS) and fly counts were recorded every 2 wk. Additionally, heifers were weighed at the beginning and end of the grazing period to determine ADG. On d 112 of the grazing period, iButton® temperature data loggers were attached to CIDR-blanks to record vaginal body temperature every 15 min for 14 d. Data were analyzed using the MIXED procedure of SAS with repeated measures examined for effects of hair coat phenotype, pasture (shaded or non-shaded) and time on ADG, HSS, HCS, fly count, and body temperature. Statistical significance was determined at P < 0.05. HCS, but not HSS, were greater in the normal hair phenotype heifers compared to heifers with the slick phenotype. Incorporation of shade lowered HCS of normal hair coat heifers, but not heifers with the slick hair phenotype (P < 0.05). Heifers with the slick hair phenotype in non-shaded pastures had decreased HSS compared to heifers with the normal hair phenotype (P < 0.05). A greater ADG was observed in heifers with the slick phenotype compared to normal hair phenotype (0.46 and 0.36 Kg/d, respectively; P < 0.05) as well as those cattle that were permitted shade compared to those without shade (0.48 and 0.33 Kg/d, respectively; P < 0.05). Fly counts were greatest in normal haired cattle (101.5 ± 27.6) in non-shaded pastures compared to the other animal groups (62.1,64.9, and 51.2 for slick in non-shaded, normal and slick in shaded pastures, respectively; P < 0.05). Heifers grazing in non-shaded pastures displayed a greater body temperature compared to those cattle permitted access to shade (P < 0.05). The combination of greater body temperatures, fly counts, HCS and HSS during the grazing period negatively impacted the growth performance of heifers in non-shaded pastures with the normal hair phenotype compared to the other animal groups.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Monn, R. E. and Shea, T. P. and Mayberry, K. and Freeman, S. R. and Poole, D. H. and Poore, M. H.}, year={2018}, month={Mar}, pages={79–80} }
 @article{rogers_poore_shaeffer_freeman_2018, title={On-farm workshops improve producers understanding of winter forage management strategies.}, volume={96}, ISSN={["1525-3163"]}, DOI={10.1093/jas/sky027.019}, abstractNote={In 2017 a series of 4 on-farm workshops were held across North Carolina to address winter forage management strategies. Two workshops were held in the Piedmont region where tall fescue (Lolium arundinaceum) is the dominant forage. Topics included extending the grazing season, improving soil health, frost-seeding clover, improving soil nutrient distribution and introducing warm season grasses. Two additional workshops were held in the Coastal Plain region where bermudagrass (Cynodon dactylon) is the most prevalent forage. Those sessions focused on over-seeding Bermudagrass pastures with winter annuals, renovating winter feeding areas, improving soil nutrient distribution and livestock mineral supplementation. Workshops were held on farms participating in USDA-NRCS Conservation Innovation Grant #693A75-14–251 which is focused on improving soil health in pasture-based livestock farms. Total workshop registration was 153 with 120 evaluations completed. Participants were 73% male and 27% female with 51% being under the age of 50. The total number of pasture hectares grazed by participants was 3726 with 1682 ha as hay. Livestock inventories for attendees were 5289 beef cattle, 505 sheep, 359 goats and 62 horses. Post-workshop responses from the Piedmont workshops indicated that 100% of attendees had increased understanding of using stockpiling, frost seeding clover and warm season grasses in fescue systems. Ninety-seven percent of participants indicated a better understanding of how annual forages can be used to extend the grazing season and soil nutrient distribution. Similar responses were reported from the Coastal Plain workshops with 100% of attendees having increased understanding of establishing winter annuals into Bermudagrass, winter feeding area renovation and mineral supplementation. Ninety-six percent of attendees had an improved understanding of how grazing management can impact soil nutrient distribution. Participants completed an evaluation at the conclusion of each workshop (n = 120) where they indicated their satisfaction with (scale of 1 = not satisfied to 4 = very satisfied, mean ± SD): instructors’ knowledge (3.85 ± 0.35), workshop environment/format (3.85 ± 0.37), and overall quality (3.85 ± 0.35). One hundred percent of the attendees indicated that this workshop met their expectations and they would recommend it to others. Continued workshop participation indicates the demand for hands-on education in these areas and that the on-farm format is an effective teaching environment.}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Rogers, J. R. and Poore, M. and Shaeffer, A. D. and Freeman, S. R.}, year={2018}, month={Mar}, pages={10–10} }
 @article{franzluebbers_pehim-limbu_poore_2018, title={Soil-Test Biological Activity with the Flush of CO2: IV. Fall-Stockpiled Tall Fescue Yield Response to Applied Nitrogen}, volume={110}, ISSN={["1435-0645"]}, DOI={10.2134/agronj2018.03.0146}, abstractNote={Core Ideas
Soil biological activity is a reliable indicator of soil nitrogen availability.
Nitrogen fertilization of fall stockpiled tall fescue should be adjusted based on soil testing.
The flush of CO2 is a robust indicator of soil biological activity.
A new paradigm of soil testing based on soil biological activity is possible.
Fall stockpiling of tall fescue (Lolium arundinaceum) in the southeastern United States is promoted as an ecologically favorable cattle management approach to avoid the financial and environmental burdens of winter hay feeding. We hypothesized that soil N mineralization should be an important factor controlling forage yield response to N fertilizer. We conducted 55 N fertilizer trials in combination with analyses of soil C and N fractions at multiple locations in Georgia, North Carolina, Virginia, and West Virginia during two seasons. Plant‐available N, as a combination of residual inorganic N + mineralizable N at depth of 0 to 10 cm, was significantly negatively related with extent of forage dry matter response to N fertilizer input. Large variations in economically optimum N fertilizer requirement (EONR) occurred among fields, but when several fields were averaged along a gradient of soil biological activity, a strong negative yield response with increasing soil‐test biological activity emerged. With moderate soil‐test biological activity of 200 mg CO2–C kg–1 soil 3 d–1, EONR was 20 kg N Mg–1 forage dry matter (a value similar to current N fertilizer recommendations). However, with progressively greater soil‐test biological activity up to 600 mg CO2–C kg–1 soil 3 d–1, EONR declined in a nonlinear manner to near zero. These results illustrate that N fertilizer recommendations for fall stockpiled tall fescue pastures should be a function of soil‐test biological activity as an indicator of biologically active N. Greater economic and environmental sustainability would likely be attainable with a shift to recognizing soil biological activity in an ecologically oriented fertilization paradigm.}, number={5}, journal={AGRONOMY JOURNAL}, author={Franzluebbers, Alan J. and Pehim-Limbu, Smriti and Poore, Matt H.}, year={2018}, pages={2033–2049} }
 @article{stewart_beck_walker_poore_arthington_lawrence_2016, title={BILL E. KUNKLE INTERDISCIPLINARY BEEF SYMPOSIUM: Mineral Nutrition in Beef Cattle Production}, volume={94}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2016-1116}, abstractNote={The Bill Kunkle Interdisciplinary Beef Symposium titled “The Current Status of Mineral Nutrition in Beef Cattle Production: From Pasture to Plate” was held at the annual meeting of the Southern Section of the American Society of Animal Science on Monday, February 8, 2016, in San Antonio, TX. The symposium was developed to present up-to-date information regarding mineral nutrition of beef cattle in all sectors of production through harvest as well as offer an arena for discussion on the impact of current feeding strategies and management practices on mineral status, reproduction, immunity, growth, and antemortem impacts on carcass characteristics and meat quality. The symposium comprised 4 invited presentations, all of which are briefly discussed below. The symposium began with an invited presentation by Dr. L. W. Greene (Auburn University, Auburn, AL) entitled “Assessing the current mineral supplementation needs in pasture-based beef operations in the Southeastern United States.” Dr. Greene gave an...}, number={12}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Stewart, R. L., Jr. and Beck, P. and Walker, R. S. and Poore, M. H. and Arthington, J. D. and Lawrence, T. E.}, year={2016}, month={Dec}, pages={5393–5394} }
 @article{moriel_piccolo_artioli_poore_marques_cooke_2016, title={Decreasing the frequency and rate of wet brewers grains supplementation did not impact growth but reduced humoral immune response of preconditioning beef heifers}, volume={94}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2015-0250}, abstractNote={This study evaluated growth and measurements of innate and humoral immunity of preconditioning beef heifers supplemented with wet brewers grains (WBG) at 2 supplementation rates and frequencies. At 14 d after weaning (d 0), Angus heifers ( = 36; 213 ± 2 kg BW and 254 ± 7 d of age) were stratified by BW and age and randomly assigned to 1 of 12 drylot pens (3 heifers/pen). Treatments were randomly assigned to pens, in a 2 × 2 factorial design, and consisted of heifers provided ground tall fescue hay ad libitum (55% TDN and 12% CP of DM) and supplemented with WBG (75% TDN and 36% CP of DM) either daily (7X) or 3 times weekly (3X; Monday, Wednesday, and Friday) at 0.5 or 1.0% of BW (DM basis) for 42 d. Heifers were vaccinated against infectious bovine rhinotracheitis (IBR), bovine viral diarrhea virus (BVDV), Mannheimia haemolytica, and Clostridium on d 14 and 28. Individual BW was measured before feeding on d 0 and 42 following 12 h of feed and water withdrawal. Blood samples were collected via jugular venipuncture 4 h after WBG supplementation on d 14, 15, 16, 17, 21, 28, 29, 30, 31, 35, and 42. Heifers fed WBG 3X had less hay DMI (2.6 ± 0.16 vs. 3.2 ± 0.16 kg/d; < 0.0001) but greater total DMI (5.6 ± 0.16 vs. 3.8 ± 0.16 kg/d; < 0.0001) than 7X heifers on days that all heifers received WBG supplementation. However, overall hay and total DMI was not affected ( ≥ 0.40) by supplementation frequency. Therefore, ADG, BW, and G:F from d 0 to 42 did not differ among treatments ( ≥ 0.29). Plasma concentrations of haptoglobin on d 15 and cortisol on d 14 were greater for 3X heifers vs. 7X heifers ( ≤ 0.04). Heifers fed WBG at 0.5% of BW tended to have greater plasma cortisol concentrations on d 15, 17, and 35 ( ≤ 0.09) than heifers fed at 1.0% of BW. Serum BVDV-1a titers were greater ( = 0.04) for 7X heifers vs. 3X heifers on d 42 (4.2 ± 0.28 vs. 3.3 ± 0.28 log), whereas serum titers against BVDV-2 and IBR were greater for heifers fed WBG at 1.0% of BW vs. heifers fed WBG at 0.5% of BW (7.6 vs. 6.7 and 3.3 vs. 2.8 ± 0.19 log, respectively). In summary, decreasing WBG supplementation frequency (7 vs. 3 times weekly) or rate (1.0 vs. 0.5% of BW) for recently weaned beef heifers did not affect growth but decreased vaccine-induced antibody production against pathogens associated with bovine respiratory disease during a 42-d preconditioning period.}, number={7}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Moriel, P. and Piccolo, M. B. and Artioli, L. F. A. and Poore, M. H. and Marques, R. S. and Cooke, R. F.}, year={2016}, month={Jul}, pages={3030–3041} }
 @article{lyons_shaeffer_drewnoski_poore_poole_2016, title={Effect of protein supplementation and forage allowance on the growth and reproduction of beef heifers grazing stockpiled tall fescue}, volume={94}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2015-9969}, abstractNote={Stockpiled tall fescue can provide adequate winter forage for beef cattle, although unsupplemented replacement heifers may display marginal performance before breeding. The objective of this study was to determine if protein supplementation and/or additional forage improves growth and reproductive performance of replacement heifers grazing stockpiled fescue. Cattle averaging 272 ± 1.59 kg were stratified by BW and then randomly assigned to 1 of 4 plots within a pasture replication. Treatment combinations were assigned in a 2 × 2 factorial arrangement and included 1) a conservative forage allocation ("normal," targeting 85% forage use) and mineral supplement (normal forage allocation with mineral supplement [FM]), 2) normal forage allocation with protein tub (FT), 3) more liberal forage allocation ("extra," targeting 70% forage use) and mineral supplement (extra forage allocation with mineral supplement [EM]), and 4) "extra forage allocation with protein tub (ET). Treatments were administered for 8 wk from early November to early January. Heifers were fed fescue hay for 1 wk before breeding in late January. Heifers were synchronized with the 7-d CO-Synch + controlled internal drug release device protocol and inseminated in late January. Heifers were checked for pregnancy by ultrasonography at 35 and 90 d after AI. Main and interaction effects between the 2 treatments were determined. Total supplement intake was greater for protein tub than mineral supplement (0.36 vs. 0.11 kg·heifer·d, respectively; < 0.0001), and the additional dietary protein in the tub groups resulted in greater serum urea N concentrations ( < 0.0001; 8.15 vs. 10.4 mg/dL for mineral and protein tub, respectively). Forage utilization efficiency was greater for normal than extra forage allocation (74.7 vs. 65.8%, respectively; < 0.0001). Main effects of both treatments on ADG were significant ( < 0.0001; 0.28, 0.43, 0.43, and 0.51 kg·heifer·d for FM, FT, EM, and ET, respectively). There was an interaction effect of the 2 treatments on change in BCS ( < 0.05; 0.12, 0.10, 0.18, and 0.31 for FM, FT, EM, and ET, respectively). Reproductive tract scores, pelvic area, and AI pregnancy rates were not different between treatments ( > 0.05). Overall, feeding a protein supplement or providing extra forage increased gain and interacted to increase BCS but did not have an effect on reproductive performance. Supplementing with protein and providing extra forage are strategies that can increase gain in heifers, which could aid heifers in reaching puberty before estrous synchronization.}, number={4}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Lyons, S. E. and Shaeffer, A. D. and Drewnoski, M. E. and Poore, M. H. and Poole, D. H.}, year={2016}, month={Apr}, pages={1677–1688} }
 @article{moriel_artioli_piccolo_marques_poore_cooke_2016, title={Effects of timing of anabolic implant insertion on growth and immunity of recently weaned beef steers}, volume={94}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2016-0470}, abstractNote={We evaluated the effects of timing of estrogenic implant insertion, relative to weaning, on growth performance and measurements of innate and humoral immunity of beef calves. On d -14, Angus × Simmental crossbred steers ( = 48; BW = 217 ± 5 kg; age = 191 ± 3 d) were stratified by BW, age, and cow parity and randomly assigned to receive no implant (NOIP) or 36 mg of zeranol on d -14, 0, or 14, relative to weaning (IP-14, IP0, and IP+14, respectively; 12 steers/treatment). From d -14 to 0, cow-calf pairs remained on a single, tall-fescue pasture with no access to concentrate supplementation. Steers were weaned on d 0, stratified by treatment and BW, and then allocated into 1 of 16 drylot pens to receive daily free-choice access to a corn silage-based diet during the preconditioning phase (d 0 to 56). Steers were vaccinated against infectious bovine rhinotracheitis (IBRV), bovine viral diarrhea virus (BVDV), and on d -27 and 0. From d 56 to 252 (postpreconditioning phase), steers remained in their respective feedlot pens and were provided free-choice access to corn silage-based growing (d 56 to 167) and finishing total mixed rations (d 168 to 252). Body weight on d 0 did not differ among treatments ( ≥ 0.29) but was greater for IP-14 and IP0 than NOIP and IP+14 steers on d 14, 42, and 56 ( ≤ 0.05). Treatment effects were not detected for G:F and DMI from d 0 to 56 ( ≥ 0.34), but ADG from d -14 to 56 was greater for IP-14 compared to NOIP ( ≤ 0.05) and intermediate for IP0 and IP+14 steers. Plasma IGF-1 concentrations were greater for IP-14 than NOIP ( ≤ 0.05) and intermediate for IP0 and IP+14 steers on d -7, 0, 14, and 21. Plasma concentrations of cortisol and haptoglobin and serum titers against BVDV types 1a and 2 did not differ among treatments from d 0 to 56 ( ≥ 0.37). However, serum IBRV titers were greater for IP+14 than NOIP, IP-14, and IP0 steers ( ≤ 0.02). On d 252, BW was greater for IP-14 and IP0 than NOIP steers ( ≤ 0.05) and intermediate for IP+14 steers, but ADG and G:F from d 57 to 252 and carcass characteristics at slaughter did not differ among treatments ( ≥ 0.16). Thus, the 36-mg zeranol implant did not elicit an inflammatory response or affect the overall vaccine response of steers (except for IBRV titers). However, growth of steers during a 56-d preconditioning period was enhanced by administering 36-mg zeranol implant 14 d before weaning, without affecting subsequent postpreconditioning growth and carcass characteristics at slaughter.}, number={7}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Moriel, P. and Artioli, L. F. A. and Piccolo, M. B. and Marques, R. S. and Poore, M. H. and Cooke, R. F.}, year={2016}, month={Jul}, pages={3051–3060} }
 @article{moriel_artioli_piccolo_marques_poore_cooke_2016, title={Frequency of wet brewers grains supplementation during late gestation of beef cows and its effects on offspring postnatal growth and immunity}, volume={94}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2016-0427}, abstractNote={Our objectives were to evaluate postnatal growth and measurements of innate and humoral immunity of beef calves born to dams fed wet brewers grains (WBG) daily or 3 times weekly during late gestation. On d 0 (approximately 60 d before calving), 28 multiparous, spring-calving Angus cows (BW = 578 ± 19 kg; age = 4.7 ± 0.65 yr; BCS = 7.0 ± 0.18) were stratified by sire, age, BW, and BCS and then randomly allocated into 1 of 14 drylot pens (2 cows/pen; 18 by 3 m; 27 m/cow). Cows were offered ground tall fescue hay ad libitum and received similar weekly WBG supplementation (DMI = 0.5% of BW multiplied by 7 d). Treatments were randomly assigned to pens (7 pens/treatment) and consisted of cows receiving WBG supplementation daily (S7; weekly DMI of WBG divided by 7 d) or 3 times weekly (S3; weekly DMI of WBG divided by 3 d; Mondays, Wednesdays, and Fridays) from d 0 until calving. Cow-calf pairs were managed as a single group on tall fescue pastures from calving to weaning (d 226). Calves were immediately submitted to a preconditioning period from d 226 to 266 and vaccinated against infectious bovine rhinotracheitis, bovine viral diarrhea virus, , and on d 231 and 245. Decreasing the frequency of WBG supplementation did not impact ( ≥ 0.21) precalving intake of total DM, CP, and TDN; BW and BCS change; overall plasma cortisol concentrations; and postcalving growth and pregnancy rate of cows. Overall plasma concentrations of glucose and insulin did not differ ( ≥ 0.28) between S3 and S7 cows, whereas S3 cows had greater ( = 0.002) plasma glucose concentrations and tended ( = 0.06) to have greater plasma insulin concentrations on days they were not fed WBG vs. days of WBG supplementation. Calf plasma concentrations of haptoglobin and cortisol at birth but not serum IgG ( = 0.63) tended ( = 0.10) to be greater for S3 vs. S7 calves. However, additional calf growth and immunity variables obtained during pre- and postweaning phases did not differ between S3 and S7 calves ( ≥ 0.21). Hence, decreasing the frequency of WBG supplementation during late gestation caused oscillations on precalving plasma glucose and insulin concentrations but did not affect plasma cortisol concentrations, growth, and pregnancy rate of cows. Also, reduced frequency of WBG supplementation during late gestation did not have carryover effects on postnatal calf growth and immunity.}, number={6}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Moriel, P. and Artioli, L. F. A. and Piccolo, M. B. and Marques, R. S. and Poore, M. H. and Cooke, R. F.}, year={2016}, month={Jun}, pages={2553–2563} }
 @article{moriel_piccolo_artioli_marques_poore_cooke_2016, title={Short-term energy restriction during late gestation of beef cows decreases postweaning calf humoral immune response to vaccination}, volume={94}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2016-0426}, abstractNote={Our objectives were to evaluate the pre- and postweaning growth and measurements of innate and humoral immune response of beef calves born to cows fed 70 or 100% of NEm requirements during the last 40 d of gestation. On d 0 (approximately 40 d before calving), 30 multiparous Angus cows pregnant to embryo transfer (BW = 631 ± 15 kg; age = 5.2 ± 0.98 yr; BCS = 6.3 ± 0.12) were randomly allocated into 1 of 10 drylot pens (3 cows/pen). Treatments were randomly assigned to pens (5 pens/treatment) and consisted of cows limit-fed (d 0 to calving) isonitrogenous, total-mixed diets formulated to provide 100 (CTRL) or 70% (REST) of daily NEm requirements of a 630-kg beef cow at 8 mo of gestation. Immediately after calving, all cow-calf pairs were combined into a single management group and rotationally grazed on tall fescue pastures (6 pastures; 22 ha/pasture) until weaning (d 266). All calves were assigned to a 40-d preconditioning period in a drylot from d 266 to 306 and vaccinated against infectious bovine rhinotracheitis, bovine viral diarrhea virus (BVDV), , and spp. on d 273 and 287. Blood samples from jugular vein were collected from cows on d 0, 17, and 35 and from calves within 12 h of birth and on d 266, 273, 274, 276, 279, and 287. By design, REST cows consumed less ( ≤ 0.002) total DMI, TDN, and NEm but had similar CP intake ( = 0.67), which tended ( = 0.06) to increase BW loss from d 0 to calving, than CTRL cows (-1.09 vs. -0.70 ± 0.14 kg/d, respectively). However, gestational NEm intake did not affect ( ≥ 0.30) plasma concentrations of cortisol, insulin, and glucose during gestation and BCS at calving as well as postcalving pregnancy rate, BW, and BCS change of cows. Calf serum IgG concentrations and plasma concentrations of haptoglobin and cortisol at birth as well as calf pre- and postweaning BW and ADG did not differ ( ≥ 0.15) between calves born to REST and CTRL cows. However, calf postweaning overall plasma concentrations of cortisol; plasma haptoglobin concentrations on d 274, 276, and 279; and serum BVDV-1a titers on d 306 were less for REST calves than for CTRL calves ( ≤ 0.05). Hence, a NEm restriction to 70% of daily requirements during the last 40 d of gestation had minimal effects on cow precalving growth and did not affect postcalving cow growth and reproductive performance. However, it decreased postweaning vaccination-induced humoral immunity, inflammatory, and physiological stress responses of calves.}, number={6}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Moriel, P. and Piccolo, M. B. and Artioli, L. F. A. and Marques, R. S. and Poore, M. H. and Cooke, R. F.}, year={2016}, month={Jun}, pages={2542–2552} }
 @article{scaglia_beck_poore_lehmkuhler_2015, title={BILL E. KUNKLE INTERDISCIPLINARY BEEF SYMPOSIUM: Tall fescue toxicosis update}, volume={93}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2015-9892}, abstractNote={The Bill Kunkle Interdisciplinary Beef Symposium entitled “Tall Fescue Toxicosis Update” was held at the annual meeting of the Southern Section of ASAS on Monday, February 2, 2015, in Atlanta, GA. The purpose of the symposium was to present up-to-date information regarding fescue toxicosis in beef cattle and new information regarding metabolism of ergot alkaloids, as well as to provide an environment for discussion of years of research focusing on how to reduce the negative effects of this syndrome and improve genetic resistance to it. The symposium was comprised of 5 invited presentations, which are briefly discussed below. The symposium began with an invited presentation by R. L. Kallenbach (University of Missouri, Columbia, MO) entitled “Coping with tall fescue toxicosis: Solutions and realities”. During his presentation, Kallenbach gave an overview of the current status of the possible alternatives to reduce the effect of fescue toxicosis in beef cattle with emphasis on agronomic and management practices (Kallenbach, 2015). Testing tall fescue for infection rate is of major importance due to its impact on animal productivity and well-being. Based on these tests published in several scientific journals, it has been concluded that ADG of stocker cattle is reduced 45 g for every 10% increase in endophyte infection and cow conception rates decreased 3.5% for every 10% increase on the pastures infestation rates.}, number={12}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Scaglia, G. and Beck, P. and Poore, M. H. and Lehmkuhler, J.}, year={2015}, month={Dec}, pages={5485–5486} }
 @article{artioli_moriel_poore_marques_cooke_2015, title={Decreasing the frequency of energy supplementation from daily to three times weekly impairs growth and humoral immune response of preconditioning beef steers}, volume={93}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2015-9457}, abstractNote={We evaluated the effects of frequency of energy supplementation on growth and measurements of innate and humoral immune responses of preconditioning beef steers following vaccination. Angus steers ( = 24; 221 ± 6.3 kg; 177 ± 4 d of age) were weaned on d -7 and kept in a single drylot pen with free access to tall fescue hay and concentrate DMI at 0.5% of BW (50:50 mix of soyhulls and corn gluten pellets; DM basis) from d -7 to 0. On d 0, steers were stratified by BW and age and randomly assigned to 1 of 8 feedlot pens (3 steers/pen). Treatments were randomly assigned to pens (4 pens/treatment) and consisted of steers provided daily free access to ground tall fescue hay and similar weekly concentrate DMI (1% of BW times 7 d), which was divided and offered either daily (S7) or 3 times weekly (S3; Monday, Wednesday, and Friday) from d 0 to 42. Individual BW was measured before feeding on d 0 and 42, after 12 h of feed and water withdrawal. Steers were vaccinated against infectious bovine rhinotracheitis, bovine viral diarrhea virus (BVDV), and clostridium on d 7 and 21. Blood samples were collected from the jugular vein on d -7 and 4 h after concentrate supplementation on d 0, 7, 8, 9, 10, 14, 21, 22, 23, 24, 28, 35, and 42. Steers offered concentrate daily had greater ( ≤ 0.02) BW on d 42, overall ADG, and total DMI, but similar ( = 0.14) G:F, than S3 steers. On days that S7 and S3 steers were offered concentrate, total DMI was greater and hay DMI was less for S3 vs. S7 steers ( ≤ 0.05). On days that only S7 steers were supplemented, hay DMI was greater, but total DMI was less for S3 vs. S7 steers ( ≤ 0.05). Mean CP and NEg intake were greater ( ≤ 0.03) for S7 vs. S3 steers. Plasma cortisol concentrations on d 7 and 28, and mean plasma haptoglobin concentrations, but not liver mRNA expression of haptoglobin ( = 0.75), were greater for S3 vs. S7 steers ( ≤ 0.03). Plasma IGF-1 concentrations on d 0 and urea nitrogen on d 1 and 3, relative to vaccination, were greater for S7 vs. S3 steers ( ≤ 0.008). Positive seroconversion to BVDV-1b on d 42 and mean serum BVDV-1b titers were greater for S7 vs. S3 steers ( ≤ 0.05). In summary, decreasing the frequency of concentrate supplementation from daily to three times weekly, during a 42-d preconditioning period, decreased growth performance, increased plasma concentrations of haptoglobin and cortisol, and decreased vaccine-induced antibody production against BVDV-1b of beef steers.}, number={11}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Artioli, L. F. A. and Moriel, P. and Poore, M. H. and Marques, R. S. and Cooke, R. F.}, year={2015}, month={Nov}, pages={5430–5441} }
 @article{poore_lehmkuhler_2015, title={FORAGES AND PASTURES SYMPOSIUM: Use of marginal lands and fibrous byproducts in efficient beef and dairy production systems}, volume={93}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2015-9127}, abstractNote={The Forages and Pastures Symposium “Use of Marginal Lands and Fibrous Byproducts in Efficient Beef and Dairy Production Systems” was held at the Joint Annual Meeting of the American Dairy Science Association, the American Society of Animal Science, and the Canadian Society of Animal Science in Kansas City, MO, July 20 to 24, 2014. The purpose of the symposium was to explore how the efficiency of beef and dairy production on marginal lands and using marginal-quality harvested feeds can be improved. In the future, grazing and forage production will be relegated to more marginal land and confinement feeding systems will be increasingly based on fibrous byproducts. Four invited presentations were planned to explore 1) strategies to improve the efficiency of production systems using grazing beef and dairy cattle, 2) application of improved grazing management to improve long-term productivity through improved soil health and associated ecosystem services, 3) advances in the use of fibrous byproducts with a focus on the use of corn crop residues, and 4) selection of new forage species for improved productivity on marginal lands.}, number={6}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poore, M. H. and Lehmkuhler, J. W.}, year={2015}, month={Jun}, pages={2607–2608} }
 @article{moriel_artioli_poore_confer_marques_cooke_2015, title={Increasing the metabolizable protein supply enhanced growth performance and led to variable results on innate and humoral immune response of preconditioning beef steers}, volume={93}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2015-9238}, abstractNote={We evaluated the effects of MP supply on growth performance before and after preconditioning and measurements of innate and humoral immune response of beef steers following vaccination. Angus steers ( = 36; BW = 231 ± 21 kg; age = 184 ± 18 d) were weaned on d -6, stratified by BW and age on d 0, and randomly assigned to 1 of 18 drylot pens (2 steers/pen). Treatments were assigned to pens (6 pens/treatment) and consisted of corn silage-based diets formulated to provide 85%, 100%, or 115% of the daily MP requirements of a beef steer gaining 1.1 kg/d from d 0 to 42. Steers were vaccinated against infectious bovine rhinotracheitis virus, bovine viral diarrhea (BVDV) types 1 and 2 viruses, and clostridium on d 14 and 28. Blood samples were collected on d 0, 14, 15, 17, 21, 28, 29, 30, 35, and 42. Body weight did not differ ( ≥ 0.17) among treatments from d 0 to 28. On d 42, 115% MP steers were heaviest, 100% MP steers were intermediate, and 85% MP steers were lightest ( = 0.05; 297, 290, and 278 ± 7 kg, respectively). Overall, ADG and G:F did not differ ( ≥ 0.13) between 100% and 115% MP steers and were least ( < 0.01) for 85% MP steers (1.2, 1.4, and 0.8 ± 0.07 kg/d and 0.23, 0.24, and 0.19 ± 0.008, respectively). Plasma haptoglobin (Hp) concentrations did not differ among treatments ( ≥ 0.46), whereas plasma ceruloplasmin (Cp) concentrations were greatest ( ≤ 0.04) for 85% MP steers, intermediate for 100% MP steers, and least for 115% MP steers on d 30, 35, and 42. Plasma cortisol concentrations were greater ( ≤ 0.03) for 85% vs. 100% and 115% MP steers on d 14 and 28. Liver mRNA expression of Cp and Hp and muscle mRNA expression of m-calpain, mammalian target of rapamycin, and ubiquitin did not differ among treatments ( ≥ 0.17). Serum neutralization titers to BVDV-1b titers were greater ( ≤ 0.02) for 115% vs. 85% and 100% MP steers on d 42 (5.8, 3.0, and 3.7 ± 0.60 log, respectively), whereas mean serum leukotoxin titers were greater for 85% vs. 100% and 115% MP steers (3.1, 2.4, and 2.5 ± 0.21 log, respectively). Preconditioning MP supply did not affect ( ≥ 0.26) ubsequent finishing growth performance and carcass characteristics. Thus, increasing MP supply from 85% to 115% of daily requirement of preconditioning beef steers had variable results on innate and humoral immune response and enhanced growth performance during a 42-d preconditioning period without affecting carcass characteristics at slaughter.}, number={9}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Moriel, P. and Artioli, L. F. A. and Poore, M. H. and Confer, A. W. and Marques, R. S. and Cooke, R. F.}, year={2015}, month={Sep}, pages={4473–4485} }
 @article{bordeaux_grossman_white_osmond_poore_pietrosemoli_2014, title={Effects of rotational infrastructure within pasture-raised pig operations on ground cover, soil nutrient distribution, and bulk density}, volume={69}, ISSN={["1941-3300"]}, DOI={10.2489/jswc.69.2.120}, abstractNote={Interest in pasture-based pork products has increased significantly in recent years. However, nitrogen (N) losses resulting from these systems are common due to importation of feed, high stocking rates, and pig behavior. This study was conducted to evaluate soil inorganic N, soil-test phosphorus (STP), ground cover, and compaction changes as impacted by rotational shade, water, and feed structures in a pasture-raised pig operation over two 12-week pig occupations. Shade and watering structures were rotated weekly for 12 weeks within a rotational (mobile) scheme; data were compared to a stationary structure system as well as to a managed hay operation with no pigs. Soil samples were acquired from subplots and analyzed for distribution of inorganic N concentrations among main plot treatments, including nitrate (NO3), ammonium (NH4), and STP values. Soil inorganic N concentrations were higher in exterior subplot positions than in interior positions. This pattern was not maintained after a second pig group occupied the plots. Soil test phosphorus was unaffected by either pig occupation. Ground cover percentages were higher in control (hay) treatments than for pig treatments, however no difference was found between mobile and stationary structure treatments in either pig occupation. Soil compaction, as measured by soil bulk density, was found to be higher under permanent shade structure locations as compared to mobile and control treatments. Mobile and control compaction levels were not different for the second occupation, utilizing a more intensive sampling scheme, suggesting a benefit to the rotation of shade, water and feed infrastructure. The weekly rotation of infrastructure performed during both occupations was both labor intensive and time consuming. The observed lack of improvement in nutrient distribution to a rotational infrastructure may limit its utility in pastured-pig systems. However, further options are available that would allow the production of pasture-raised pigs while minimizing associated nutrient loading and pasture degradation.}, number={2}, journal={JOURNAL OF SOIL AND WATER CONSERVATION}, publisher={Soil and Water Conservation Society}, author={Bordeaux, C. and Grossman, J. and White, J. and Osmond, D. and Poore, M. and Pietrosemoli, S.}, year={2014}, pages={120–130} }
 @article{drewnoski_huntington_poore_2014, title={Reduced supplementation frequency increased insulin-like growth factor 1 in beef steers fed medium quality hay and supplemented with a soybean hull and corn gluten feed blend}, volume={92}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2013-7372}, abstractNote={Reducing supplementation frequency in calf growing programs can reduce labor and equipment operation costs. However, little is understood about the metabolic response of ruminants to large fluctuations in nutrient intake. Eighteen Angus or Angus × Simmental cross steers (287 ± 20 kg and 310 ± 3.6 d of age) were individually fed 1 of 3 dietary treatments using Calan gates. Dietary treatments consisted of ad libitum hay and no supplement (NS), ad libitum hay and 1% BW (as-fed basis) of supplement daily (DS), or ad libitum hay and 2% BW (as-fed basis) of supplement every other day (SA). The supplement was 90% DM and contained (as-fed basis) 47% corn gluten feed, 47% soybean hulls, 2% feed grade limestone, and 4% molasses. Hay intake and ADG was measured over a 52-d period. Steers were then moved to individual tie stalls. Steers were fed at 0800 h and blood samples were collected every hour from 0600 to 1400 h and at 1800, 2200, and 0200 h over a 2-d period. Gains were increased (P < 0.01) by supplementation but did not differ (P = 0.68) due to supplementation frequency. Average daily gain was 0.45, 0.90, and 0.87 kg ·hd(-1)·d(-1) (SEM ± 0.05) for steers NS, DS, and SA, respectively. Across the 2-d supplementation cycle area under the concentration time curve (AUC) for plasma glucose was increased (P < 0.01) by supplementation but did not differ (P = 0.41) due to supplementation frequency. The AUC for plasma insulin was increased by supplementation (P < 0.01) but did not differ (P = 0.67) due to supplementation frequency. Plasma IGF-1 was increased (P = 0.01) by supplementation and was greater (P = 0.04) for steers supplemented SA than DS. Gains of steers supplemented with a soybean hull and corn gluten feed blend on alternate days did not differ from those supplemented daily suggesting the steers were able to efficiently utilize large boluses of nutrients fed every other day. The effect of less frequent supplementation on IGF-1 deserves further examination as this hormone has been shown to increase protein synthesis.}, number={6}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Drewnoski, M. E. and Huntington, G. B. and Poore, M. H.}, year={2014}, month={Jun}, pages={2546–2553} }
 @article{poore_washburn_2013, title={FORAGES AND PASTURES SYMPOSIUM: Impact of Fungal Endophytes on Pasture and Environmental Sustainability}, volume={91}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2013-6431}, abstractNote={The discovery of endophyte-grass associations in tall fescue [Lolium arundinaceum (Schreb.) Darbysh. syn. Festuca arundinacea Schreb.] and perennial ryegrass (Lolium perenne L.) has improved the understanding of fescue toxicosis, ryegrass staggers, and other related syndromes. These two forage species are of major importance in temperate grazing systems, and the presence of alkaloid toxins in many varieties produced by endophytic fungi (from the Epichloë and Neotyphodium genera) has led to poor animal performance and great economic losses. About 8 million beef cows in the United States graze tall fescue sometime during the year, and there are also significant numbers of dairy cattle, horses, and small ruminants present in the fescue belt. Total economic loss as a result of adverse effects on growth rate, reproduction, and milk production exceeds $1 billion/yr (Roberts and Andrae, 2010). Recent development and commercialization of tall fescue infected with an endophyte that does not produce toxic alkaloids (e.g., MaxQ, Pennington Seed, Madison, GA) represents the first use of endophyte technology to enhance the agronomic performance of a grass (i.e., Jesup tall fescue). Initial research demonstrated that agronomic characteristics of tall fescue infected with nontoxic endophytes were better than endophyte-free tall fescue and not different from tall fescue infected with wild-type (i.e., toxic) endophyte. Furthermore, animal performance was similar for endophyte-free and nontoxic-endophyte-infected tall fescue, both of which supported improved performance vs. tall fescue infected with wild-type endophyte (Parish et al., 2003). Questions remained about how long-term persistence of the nontoxic-endophyte-infected tall fescue would compare with wild-type-infected tall fescue. Two long-term studies have been published recently (Vibart et al., 2008; Drewnoski et al., 2009a,b; Franzluebbers et al., 2009) that confirmed the beneficial effects of the nontoxic endophyte on animal performance and also showed that long-term yield and persistence were improved compared with endophyte-free fescue and were nearly equal to tall fescue infected with wild-type endophyte.}, number={5}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poore, M. H. and Washburn, S. P.}, year={2013}, month={May}, pages={2367–2368} }
 @article{sullivan_freeman_van heugten_ange-van heugten_wolfe_poore_2012, title={Impact of two types of complete pelleted, wild ungulate feeds and two pelleted feed to hay ratios on the development of urolithogenic compounds in meat goats as a model for giraffes}, volume={97}, ISSN={0931-2439}, url={http://dx.doi.org/10.1111/j.1439-0396.2012.01297.x}, DOI={10.1111/j.1439-0396.2012.01297.x}, abstractNote={SummaryUrolith formation has been documented in giraffes and goats. As research in giraffes poses logistical challenges, 16 buck goats were used as a model. The impact of two commercially available, pelleted feeds used for giraffes, ADF‐16 and Wild Herbivore (WH), as well as the impact of alfalfa hay and pellet proportions (20% hay:80% pellets, 80P or 80% hay:20% pellet, 20P) on the formation of urolithogenic precursors in goat urine was accomplished in a 2 × 2 factorial balance study. Complete diets contained 0.60, 0.32, 0.35 and 0.26% phosphorus (P) with calcium:P ratios of 1.60, 4.16, 3.06 and 5.23, for 80P‐ADF‐16, 20P‐ADF‐16, 80P‐WH and 20P‐WH respectively. Total faeces and urine were collected over two 5‐day periods to assess N and mineral balance. Fresh urine samples were collected and evaluated microscopically for urolithic crystal content. Urinary nitrogen (N) was lower and N retention was higher in goats fed 80P diets (p < 0.05). Intake of P was greatest for goats fed 80P‐ADF‐16; however, urinary P excretion and P retention were not affected by treatment. Crystal scores were higher in animals receiving 80P diets (p = 0.08), with crystals being composed predominantly of calcium phosphate. Urine pH was alkaline (>8) for all treatments. Urinary P concentration, a risk factor for urolithiasis, was highest (p ≤ 0.06) in the 80P‐ADF‐16 treatment (0.38 vs. 0.01, 0.02 and 0.04 mg/dl for 20P‐ADF‐16, 80P‐WH and 20P‐WH respectively), reflecting its highest dietary P level. Further investigation is recommended to determine the long‐term effects of these diets on urolithogenic compound formation.}, number={3}, journal={Journal of Animal Physiology and Animal Nutrition}, publisher={Wiley}, author={Sullivan, K. and Freeman, S. and van Heugten, E. and Ange-van Heugten, K. and Wolfe, B. and Poore, M. H.}, year={2012}, month={Apr}, pages={566–576} }
 @article{rhinehart_poore_2013, title={SOUTHERN SECTION INTERDISCIPLINARY BEEF CATTLE SYMPOSIUM: Opportunities for stocker cattle in the southern United States}, volume={91}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2012-6095}, abstractNote={Section annual meeting has been a well-attended addition to the program at the Southern Association of Agricultural Scientists (SAAS) annual conference. This venue allows broad participation by all attendees from the Southern Section of American Society of Animal Science (ASAS), and the symposium is also well attended by members of other societies associated with SAAS. The symposium in 2012, which was held in Birmingham, AL, on February 6, was developed to address economic and production management issues for modern stocker cattle production systems. Beef cattle production in the southern United States is driven by fl uctuations in multiple commodity markets, seasonality, weather patterns, production management capabilities, and many other factors. Taking advantage of the opportunities these fl uc-tuations present can be a key component of profi tability. The average age of cattle producers in the United States is 58 yr (USDA-NASS, 2007), indicating a reduction in young producers choosing this fi eld as a career. Stocker cattle production might provide an ideal entry into the industry. Cash fl ow can be more ideally managed for a beginning producer that might lack the fi nan-cial and resource capitol for cow–calf production. However , fi nancial and production risk management can be more intense. Moreover, health, production, and pasture management require a great deal of experiential knowledge compared with cow–calf production systems. The nomenclature around stocker cattle production can be confusing and regional jargon varies widely. In general, the term " stocker " refers to a weaned calf grown predominantly on standing forage with or without supplementation (Johnson et al., 2010). " Backgrounding " refers to growing weaned calves with a high concentrate ration, often in drylot housing. The term " feeder calf " is usually intended to describe weaned steers and heifers that are going straight to a feedlot without going through a stockering or back-grounding system. The stocker cattle industry in the Southern United States is based on several different scenarios: 1) purchasing mismanaged cattle, improving their health and appearance, and then selling them to feeders as a value-added product, 2) purchasing high-quality calves as small groups or singles, commingling them into uniform lots, and profi ting from improved marketing power , or 3) adding BW on forages or byproducts at a lower cost than could be done in the feedlot. A classic model has been to purchase intact males, castrate them, and then resell them after they have healed …}, number={1}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Rhinehart, J. D. and Poore, M. H.}, year={2013}, month={Jan}, pages={501–502} }
 @article{dowler_siciliano_pratt-phillips_poore_2012, title={Determination of Pasture Dry Matter Intake Rates in Different Seasons and Their Application in Grazing Management}, volume={32}, ISSN={0737-0806}, url={http://dx.doi.org/10.1016/j.jevs.2011.06.006}, DOI={10.1016/j.jevs.2011.06.006}, abstractNote={Eight mature horses weighing 576 ± 32 kg (mean ± SD) were used to compare differences in pasture dry matter (DM) intake rate in October (period 1), February (period 2), and May (period 3). Horses were randomly assigned to a pair of adjacent 5 m × 5 m grazing cells containing nontoxic, endophyte-infected tall fescue. Horses had access to each cell for 4 hours. Pasture DM intake rate was estimated over the entire 8-hour period by measuring the pre- and postgrazing herbage mass within each cell and was expressed as kg DM/100 kg body weight (BW)/hr. Mean 8-hour DM intake rate in period 1 (0.17 ± 0.01 kg DM/100 kg BW/hr) was greater (P < .001) than for period 2 (0.09 ± 0.01 kg DM/100 kg BW/hr) and period 3 (0.11 ± 0.01 kg DM/100 kg BW/hr), but it was not different (P = .274) between periods 2 and 3. A second experiment using the same eight horses was conducted immediately after the first experiment, within each season, to determine whether the DM intake rates derived from the first experiment could be used along with estimates of maintenance digestible energy (DE) requirements and pasture DE concentrations to predict the amount of grazing time required for a horse to consume only its maintenance DE requirement and maintain zero BW change over a 6-week period. Grazing time necessary to maintain zero BW change was accurately predicted for period 1 only.}, number={2}, journal={Journal of Equine Veterinary Science}, publisher={Elsevier BV}, author={Dowler, Lauren E. and Siciliano, Paul D. and Pratt-Phillips, Shannon E. and Poore, Matthew}, year={2012}, month={Feb}, pages={85–92} }
 @article{drewnoski_poore_benson_2011, title={Effect of frequency of supplementation of a soyhulls and corn gluten feed blend on hay intake and performance of growing steers (vol 164, pg 38, 2011)}, volume={168}, ISSN={["0377-8401"]}, DOI={10.1016/j.anifeedsci.2011.04.056}, number={1-2}, journal={ANIMAL FEED SCIENCE AND TECHNOLOGY}, author={Drewnoski, M. E. and Poore, M. H. and Benson, G. A.}, year={2011}, month={Aug}, pages={144–144} }
 @article{drewnoski_poore_benson_2011, title={Effect of frequency of supplementation of a soyhulls and corn gluten feed blend on hay intake and performance of growing steers}, volume={164}, ISSN={["0377-8401"]}, DOI={10.1016/j.anifeedsci.2010.11.022}, abstractNote={Abstract Feeding supplements less frequently can reduce labor costs and increase profitability. However reducing the frequency of delivery of grain based supplements can negatively affect fiber digestion in the rumen because more grain must be fed per feeding potentially causing decreased ruminal pH and/or an insufficiency of ruminal nitrogen. Using supplements that have a moderate crude protein (CP) level, and that are low in starch, might alleviate negative effects on fiber digestion and therefore decrease negative effects associated with supplementing energy supplements less frequently. Corn gluten feed and soybean hulls are high in metabolizable energy but low in fat and starch, and corn gluten feed is also a good source of ruminally degradable CP. The objective of this study was to determine effects of reducing supplementation frequency during a weekly cycle on steer performance when supplementing medium quality hay with a blend of soybean hulls and corn gluten feed (SH/CGF). The 86 d feeding experiment was replicated over 4 years. Each year, 40 steers (BW = 263 ± 2.5, 281 ± 2.5, 271 ± 2.9, 229 ± 2.9 kg for years 1–4, respectively) were stratified by body weight and assigned to 8 groups which were randomly assigned to treatment. During years 1 and 2, treatments consisted of ad libitum medium quality fescue hay (70–100 g/kg CP and 340–410 g/kg acid detergent fiber) that was either not supplemented (HAY), supplemented daily (7X) with 2.73 kg/hd, or supplemented on Monday, Wednesday and Friday (3X) with 6.36 kg/hd. During years 3 and 4, an additional treatment was added in which steers were supplemented on Monday and Thursday with 9.55 kg/hd of SH/CGF (2X). Average daily gain was higher (P}, number={1-2}, journal={ANIMAL FEED SCIENCE AND TECHNOLOGY}, author={Drewnoski, M. E. and Poore, M. H. and Benson, G. A.}, year={2011}, month={Feb}, pages={38–44} }
 @article{freeman_poore_young_anderson_2010, title={Influence of calcium (0.6 or 1.2%) and phosphorus (0.3 or 0.6%) content and ratio on the formation of urolithogenic compounds in the urine of Boer-cross goats fed high-concentrate diets}, volume={93}, ISSN={["0921-4488"]}, DOI={10.1016/j.smallrumres.2010.05.007}, abstractNote={The population of meat goats in the U.S. increased dramatically between 2002 and 2007. With this increase has come an increase in the number of cases of obstructive urolithiasis being seen by veterinarians. Since this condition is often associated with imbalances in dietary Ca and P, the goal of this project was to investigate the influence of concentration and ratio of Ca and P in diets for meat goats on the formation of urolithogenic precursors. Using 8 Boer-cross, wether goats in replicated Latin squares, 4 diets containing either 0.3 or 0.6% P, 0.6 or 1.2% Ca, 0 or 2% added salt, and Ca:P ratios of 1:1 or 2:1 were offered (0.3% P, 2:1; 0.6% P, 1:1; 0.6% P, 2:1; and 0.6% P, 2:1 + salt). Serum, urine, and feces were collected in conjunction with water and feed intake measurements. All treatment groups had similar dry matter intakes (DMI; 838–887 g/d, P = 0.19) and N retention rates (6.2–7.0 g/d, P = 0.54). Water consumption per unit DMI was similar for goats eating both Ca:P = 2:1 diets without 2% added salt (2.06 g/g DMI and 1.89 g/g DMI for 0.6% P and 0.3% P, respectively). Water consumption was also similar for goats receiving the Ca:P = 1:1 (2.50 g water/g DMI) and 2% added salt diet (2.79 g water/g DMI); and, these levels were higher than those for the lower salt, 2:1 diets (P = 0.0002). Serum Ca, PO4, Mg, K, Na, Cl, HCO3, and anion gap all fell within normal ranges throughout the trial, as did serum urea N and glucose. Fecal DM was 10% lower in goats consuming 0.6% P, 1:1 than when other diets were offered (32% vs. 42%, P < 0.0001), possibly the result of changes in Na and water absorption in the gastrointestinal tract caused by high P and Ca:P imbalance. Goats consuming the 0.3% P, 2:1 diet had similar urinary crystal density scores (2.47 out of 3) to goats receiving the 0.6% P, 1:1 diet with Ca:P = 0.81 (2.31 out of 3). The 0.6% P, 2:1 diets without and with 2% added salt also had similar, but lower crystal density scores (1.50 for 0.6% P, 2:1 and 1.06 for 0.6% P, 2:1 + salt; P = .002). Goats with high crystal density scores had higher urinary P, Mg, or both. Our results suggested that the formation of urolithogenic compounds is highly complex and is the result of an interrelationship between multiple minerals in the diet, not only Ca and P. Mineral concentrations may interact with mineral imbalance to impact overall mineral and water absorption from the intestines. We also demonstrated that urolithogenic precursor crystals may easily be observed using light microscopy and suggested that the crystal precursors may provide a useful predictor of which goats may be prone to developing clinical urolithiasis.}, number={2-3}, journal={SMALL RUMINANT RESEARCH}, author={Freeman, S. R. and Poore, M. H. and Young, G. A. and Anderson, K. L.}, year={2010}, month={Oct}, pages={94–102} }
 @article{freeman_poore_middleton_ferket_2009, title={Alternative methods for disposal of spent laying hens: Evaluation of the efficacy of grinding, mechanical deboning, and of keratinase in the rendering process}, volume={100}, ISSN={["0960-8524"]}, DOI={10.1016/j.biortech.2009.01.077}, abstractNote={Besides the challenges of mortality and litter disposal, the poultry industry must find economical means of disposing of laying hens that have outlived their productive lives. Because spent hens have low market value and disposing of them by composting and burial is often infeasible, finding alternative disposal methods that are environmentally secure is prudent. The feasibility of grinding or mechanically deboning spent hens with and without prior mechanical picking was evaluated for the production of various proteinaceous by-product meals. The end products were analyzed for nutrient content and found to be high in protein (35.3–91.9% CP) and, with the exception of the feathers, high in fat (24.1–58.3%), making them potentially valuable protein and energy sources. After considering physical and economic feasibility, mechanical deboning was determined to be a logical first step for the conversion of spent hens into value-added by-product meals. Because the hard tissue fraction (primarily feathers, bones, and connective tissue) generated by mechanically deboning the hens presents the greatest challenge to their utilization as feedstuffs, attention was focused on technologies that could potentially improve the nutritional value of the hard tissue for use as a ruminant protein source. Traditional hydrolysis of this hard tissue fraction improved its pepsin digestibility from 74% to 85%; however, subsequent keratinase enzyme treatment for 1 h, 2 h, 4 h, or 20 h after steam hydrolysis failed to improve the pepsin or amino acid digestibility any further (P > 0.10). Enzyme hydrolysis did, however, increase the quantities of the more soluble protein fractions (A: 45.5, 46.6, 52.8, 51.6, and 55.8% of CP; B1: 3.2, 9.8, 6.0, 4.6, and 4.1% of CP; B2: 11.7, 18.1, 22.8, 29.6, and 22.0% of CP for 0, 1 h, 2 h, 4 h, and 20 h, respectively) and reduced quantities of the less soluble fractions (B3: 30.2, 18.1, 10.8, 5.5, and 10.2% of CP; C: 9.4, 7.5, 7.6, 8.8, and 7.9% of CP for 0, 1 h, 2 h, 4 h, and 20 h, respectively). The protein digestibility of the steam hydrolyzed hard tissue fraction from the mechanical deboning of spent hens was found to be comparable to the digestibility of feather meal, but post-hydrolysis keratinase treatment did not improve feeding value for ruminants.}, number={19}, journal={BIORESOURCE TECHNOLOGY}, author={Freeman, S. R. and Poore, M. H. and Middleton, T. F. and Ferket, P. R.}, year={2009}, month={Oct}, pages={4515–4520} }
 @article{sullivan_van heugten_ange-van heugten_poore_dierenfeld_wolfe_2009, title={Analysis of nutrient concentrations in the diet, serum, and urine of giraffe from surveyed North American zoological institutions}, volume={29}, ISSN={0733-3188 1098-2361}, url={http://dx.doi.org/10.1002/zoo.20278}, DOI={10.1002/zoo.20278}, abstractNote={AbstractThe objectives of the present research were to conduct a survey to investigate the health history and feeding practices of giraffe in captivity in North America and to obtain samples of hay, concentrate, browse, urine, and serum to compare across zoos, possible factors relating to the development of urolithiasis. Forty‐one out of 98 institutions contacted responded, representing 218 giraffe. All responding zoos fed concentrate and alfalfa hay was the primary forage. Sixty‐five percent of zoos fed browse and 43 different species of browse were listed. Six zoos reported a history of urolithiasis, seven reported wasting syndrome, and 10 reported sudden death. The median daily amount (as fed) of concentrate and hay offered were 5.45 kg (range of 2.73–9.55 kg) and 6.82 kg (range of 2.53–12.50 kg), respectively. The concentrate:hay ratio of the offered diet ranged from 0.22 to 3.47 with a median value of 0.79. Forty‐three percent of the institutions offered a ratio greater than 1:1. Samples of concentrate and hay (six zoos), serum (five zoos), and urine (seven zoos) were obtained for chemical analyses. Analyzed nutrient content of the consumed diet, measured by weighing feed and orts for three consecutive days, met recommendations for giraffe, but was excessive for crude protein and P. Concentrate:hay and serum P were positively correlated (r=0.72; P<0.05). High dietary P content and a high level of concentrate relative to hay may be contributing factors to urolith formation and warrant further investigation. Zoo Biol 29:457–469, 2010. © 2009 Wiley‐Liss, Inc.}, number={4}, journal={Zoo Biology}, publisher={Wiley}, author={Sullivan, Kathleen and van Heugten, Eric and Ange-van Heugten, Kimberly and Poore, Matthew H. and Dierenfeld, Ellen S. and Wolfe, Barbara}, year={2009}, pages={n/a-n/a} }
 @article{drewnoski_oliphant_poore_green_hockett_2009, title={Growth and reproductive performance of beef heifers grazing endophyte-free, endophyte-infected and novel endophyte-infected tall fescue}, volume={125}, DOI={10.1016/j.livsci.2009.05.003}, abstractNote={A three-year study was conducted to investigate the effects of endophyte-free (E−), endophyte-infected (E+) and novel endophyte-infected (EN) tall fescue on the growth and pregnancy rate of beef heifers during the spring. Each year, 48 beef heifers were strip-grazed on stockpiled fescue from December through February, fed fescue hay during late February to early April and then rotationally grazed on spring growth of fescue until June. At the end of the trial, heifers had been maintained on E+, E− or EN (pasture or hay) for a total of 152, 188 and 191 d in years 1, 2, and 3, respectively. In late March, heifers were synchronized using a controlled intravaginal drug-releasing device, (CIDR®) for 7 d followed by injection with PGF2α (Lutalyse®). Heatmount detectors (Kamar®) and observation for behavioral estrus were used to detect estrus for 63 d. Heifers were artificially inseminated 8 to12 h after the onset of standing estrus. Conception was determined by transrectal ultrasonography at approximately 30, 60 and 90 d after synchronization. Reproductive performance did not differ among treatments (P ≥ 0.20). Pregnancy rate was 54, 65, and 65% for E+, E− and EN, respectively. However, during the spring, growth and prolactin were decreased (P < 0.01) for heifers on E+. Gains on spring pasture were 0.24, 0.75, and 0.71 kg/d (SEM ± 0.03) for E+, E− and EN, respectively. Based on these results, the wild type endophyte-infected fescue can be used in production systems as a source of winter forage but producers should consider placing heifers on alternative forage (such as the novel endophyte-infected fescue) in the spring.}, number={2-3}, journal={Livestock Science}, author={Drewnoski, M. E. and Oliphant, E. J. and Poore, M. H. and Green, J. T. and Hockett, M. E.}, year={2009}, pages={254–260} }
 @article{freeman_poore_huntington_middleton_ferket_2009, title={Determination of nitrogen balance in goats fed a meal produced from hydrolyzed spent hen hard tissues}, volume={87}, ISSN={["0021-8812"]}, DOI={10.2527/jas.2008-1077}, abstractNote={To provide an economically viable and environmentally sound method for disposing of spent laying hens, we manufactured a proteinaceous meal from the hard tissue fraction of mechanically deboned laying hens (primarily feathers, bones, and connective tissue). We hydrolyzed the hard tissue and coextruded it with soybean hulls to create a novel feather and bone meal (FBM) containing 94.2% DM, 23.1% CP, 54.5% NDF, and 7.3% fat (DM basis). We evaluated the FBM in supplements for meat goats in which it provided 0, 20, 40, or 60% of the N added to the supplement compared with a negative control supplement with no added N source. The remainder of the N was contributed by soybean meal (SBM). Supplementation of N resulted in greater DMI than the negative control (P = 0.005), and DMI changed quadratically (P = 0.11) as FBM increased in the supplement. Digestibility of DM was similar in all diets, including the negative control (P > 0.10). Fiber digestibility increased linearly as dietary inclusion of FBM increased (P = 0.04 for NDF, P = 0.05 for ADF), probably as a result of the soybean hulls in the FBM. Nitrogen digestibility declined linearly from 60.5% with 0% FBM to 55.6% with 60% FBM (P = 0.07), but N retention changed by a quadratic function as FBM replaced SBM (P = 0.06). Negative control goats had less N digestibility (P < 0.001) and N retention (P = 0.008) than N-supplemented goats. Feather and bone meal had a greater proportion of ruminally undegradable B(3) protein than SBM (23.1 vs. 0.3% of CP, respectively). Ruminal VFA and pH were unaffected by replacing SBM with FBM, but supplying no source of N in the concentrate resulted in reduced total VFA in ruminal fluid (P = 0.04). Ruminal ammonia concentration increased quadratically (P = 0.07) as FBM increased, reflecting increased intake, and it was much less in unsupplemented goats (P < 0.001). Serum urea had less variation between 0 and 4 h after feeding in goats receiving 40 or 60% of added N as FBM in comparison with those receiving only SBM or 20% FBM. Feather and bone meal promoted a more stable rumen environment, possibly because of reduced rates of protein degradation within the rumen. A palatable by-product meal for ruminants can be made from spent laying hen hard tissue, one that supports N metabolism similar to that of traditional protein sources.}, number={3}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Freeman, S. R. and Poore, M. H. and Huntington, G. B. and Middleton, T. F. and Ferket, P. R.}, year={2009}, month={Mar}, pages={1068–1076} }
 @article{drewnoski_oliphant_marshall_poore_green_hockett_2009, title={Performance of growing cattle grazing stockpiled Jesup tall fescue with varying endophyte status}, volume={87}, ISSN={["1525-3163"]}, DOI={10.2527/jas.2008-0977}, abstractNote={The objective of this study was to evaluate the performance of growing cattle when intensively grazing stockpiled endophyte-infected (E+), endophyte-free (E-), and nontoxic endophyte-infected (EN) tall fescue during the winter. The experiment was conducted over 5 consecutive winters. In each year, plots (1 ha each, 4 per treatment) were harvested for hay in August, fertilized in September, and forage was allowed to accumulate until grazing was initiated in early December. Each year, 48 Angus-cross tester cattle (4 per plot) were given a daily allotment of forage, under strip-grazing (frontal grazing) management, with a target residual height of 5 cm. Steers were used the first year, and heifers were used in subsequent years. The grazing periods for determination of pasture ADG were 86 d (yr 1), 70 d (yr 2), 86 d (yr 3), 72 d (yr 4), and 56 d (yr 5). Pasture ADG of cattle did not differ among treatments (P = 0.13) and were 0.51, 0.59, and 0.56 kg/d (SEM 0.03) for E+, E-, and EN, respectively. Serum prolactin concentrations of heifers grazing E+ were less (P < 0.05) than those grazing E- and EN during all years except yr 2. In yr 2, E+ and E- did not differ (P = 0.11). Serum prolactin of heifers grazing E- and EN did not differ (P > 0.20) except in yr 4. During yr 4, serum prolactin of heifers grazing E- was greater (P = 0.05) than that of heifers grazing EN. Serum urea-N concentrations (SUN) tended to differ among treatments (P = 0.10) and there was a treatment x year interaction (P = 0.05). During yr 1 through 3, SUN did not differ (P > 0.15) among treatments. However, as the stands aged, E- had a greater invasion of other plant species, which increased the CP content of the sward, thus causing heifers grazing E- during yr 5 to have greater (P < 0.01) SUN than heifers grazing E+ and EN, which did not differ (P = 0.89). Forage disappearance (DM basis) did not differ (P = 0.75) among treatments and was 4.7, 4.7, and 5.0 kg/animal daily (SEM 0.27) for E+, E-, and EN, respectively. Body weight gain per hectare was greater (P = 0.04) for E+ (257 kg) than for E- (220 kg) or EN (228 kg). In most years, animal grazing days on E+ were greater than those on E- or EN. However, in yr 5, animal grazing days did not differ (P > 0.20) among treatments. The use of stockpiled E+ as a source of low-cost winter feed is a viable option for producers, whereas grazing of EN may be more beneficial during the spring and fall, when more severe negative effects of ergot alkaloids have been observed.}, number={3}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Drewnoski, M. E. and Oliphant, E. J. and Marshall, B. T. and Poore, M. H. and Green, J. T. and Hockett, M. E.}, year={2009}, month={Mar}, pages={1034–1041} }
 @article{butler_ranells_franklin_poore_green_2008, title={Runoff water quality from manured riparian grasslands with contrasting drainage and simulated grazing pressure}, volume={126}, ISSN={0167-8809}, url={http://dx.doi.org/10.1016/j.agee.2008.02.004}, DOI={10.1016/j.agee.2008.02.004}, abstractNote={Globally,management ofgrazedriparian areasiscriticallyimportanttoagriculturalsustainabilityandenvironmentalquality.However,the potential impacts of riparian grazing management on water quality are not well-documented, particularly in the southeastern USA. The objective of this work was to determine sediment and nutrient export under simulated rainfall from poorly drained and well-drained riparian soils where heavy or light grazing pressure by cattle was simulated. Plots were established on stands of existing vegetation to create grazing pressure treatments of (a) light-use (full ground cover, uncompacted), and with stands modified to establish (b) heavy-use (bare ground, compacted) treatments. Vegetation on poorly drained soils consisted of several typical wetland species (e.g., Pontederia cordata L., Juncus coriaceus Mackenzie) in the southeastern USA, whereas mixed tall fescue (Festuca arundinacea Schreb.)‐dallisgrass (Paspalum dilatatum Poir.) stands were the dominant vegetation on well-drained soils. Runoff volumewas generally greater from heavy-use than from light-use for poorly drained soils and for well-drained soils. Greater runoff volume was also observed from poorly drained soils compared to well-drained soils for both light-use and for heavy-use treatments. Light-use plots were remarkably effective at minimizing export of total suspended solids (TSS)onboth soils (<30 kg ha � 1 ). Mean total Kjeldahl P(TKP) exportwas fourfold greater from heavy-useplots than from light-use plots on both soils. While export of nitrate-nitrogen (NO3-N) was unaffected by grazing pressure and soil drainage, mean ammonium-nitrogen (NH4N) and total N (TN) export from poorly drained heavy-use plots was greater than fivefold that from well-drained light-use plots. Results indicate that livestock heavy-use areas in the riparian zone may export substantial TSS and nutrients, especially on poorly drained soils. However, when full ground cover is maintained on well-drained soils, TSS and nutrient losses may be limited. # 2008 Elsevier B.V. All rights reserved.}, number={3-4}, journal={Agriculture, Ecosystems & Environment}, publisher={Elsevier BV}, author={Butler, David M. and Ranells, Noah N. and Franklin, Dorcas H. and Poore, Matthew H. and Green, James T., Jr.}, year={2008}, month={Jul}, pages={250–260} }
 @article{freeman_poore_huntington_middleton_2008, title={Evaluation of secondary protein nutrients as a substitute for soybean meal in diets for beef steers and meat goats}, volume={86}, ISSN={["0021-8812"]}, DOI={10.2527/jas.2006-0698}, abstractNote={Finding appropriate disposal techniques for waste products is one of many challenges facing the poultry-processing industry. One waste generated in significant quantities is dissolved air floatation sludge, a product of wastewater treatment. Converting dissolved air floatation sludge into a dry feed product (meal) for incorporation into livestock feed appears to be a viable solution. This meal, called secondary protein nutrients (SPN), is high in protein (45% CP), fat (28% crude fat), and minerals. The protein consists of 85% B(2) and B(3) fractions, which are moderately to slowly degradable in the rumen, and therefore may potentially escape ruminal degradation and be available for digestion in the lower gastrointestinal tract. The goal of this research was to evaluate SPN as an alternative to traditional protein sources for ruminants by substituting it on an equivalent N basis for soybean meal in cattle and meat goat diets (0, 25, 50, 75, and 100% for cattle; 0, 20, and 40% for goats). When included in corn silage-based steer diets, increasing SPN resulted in linear and quadratic declines in both DMI and ADG (P < 0.001). Dry matter intake diminished with inclusion rates above 50%, and ADG were reduced after inclusion of SPN reached 25% of added N. Feed efficiency (the reciprocal of the efficiency of gain, which is represented by G:F) declined linearly (P < 0.001) with each incremental increase in SPN. Addition of up to 40% added N as SPN in goat diets caused no change in DMI, digestibility of DM or fiber, or N retention. Ruminal VFA concentrations showed little variation in either species. Increasing the proportion of SPN in the feed caused linear declines in ruminal NH(3) in steers (P < 0.001). Increasing SPN in goat diets, however, resulted in only a trend toward reductions of this parameter (P = 0.14). The decreases observed may have resulted from decreasing ruminal protein degradability or increasing fat caused by increasing the proportion of SPN in the feed. Urinary urea N as a percentage of urinary N showed significant declines in cattle, but not in goats, over the ranges of SPN offered. These results indicate that SPN can be included in diets for ruminants to supply up to 40% of supplemental N with little negative impact on animal performance.}, number={1}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Freeman, S. R. and Poore, M. H. and Huntington, G. B. and Middleton, T. F.}, year={2008}, month={Jan}, pages={146–158} }
 @article{butler_ranells_franklin_poore_green_2007, title={Ground cover impacts on nitrogen export from manured riparian pasture}, volume={36}, ISSN={["0047-2425"]}, DOI={10.2134/jeq2006.0082}, abstractNote={ABSTRACTMaintaining ground cover of forages may reduce the export of nitrogen (N) from pastures. The objective of this work was to determine the effect of ground cover on N export from pastured riparian areas receiving simulated rainfall. Plots were established on two adjacent sites in the North Carolina Piedmont: one of 10% slope with Appling sandy loam soils and a second of 20% slope with Wedowee sandy loam soils. Both sites had existing mixed tall fescue (Festuca arundinacea Schreb.)‐dallisgrass (Paspalum dilatatum Poir.) vegetation. Forage stands were modified to represent a range of ground cover levels: 0, 45, 70, and 95% (bare ground, low, medium, and high cover, respectively), and amended with beef steer (Bos taurus) feces and urine (∼200 kg N ha−1). For all rain events combined, mean nitrate N export was greatest from bare ground and was reduced by 34% at low cover, which did not differ from high cover. Mean ammonium N export was slightly elevated (∼1.37 kg N ha−1) in months when manures were applied and negligible (<0.02 kg N ha−1) in all other months. For all rain events combined, mean export of total N was greatest from bare ground and was reduced by at least 85% at all other cover levels. Whereas site did not impact N export, results indicated that cover and time of rainfall following manure deposition are important determinants of the impact of riparian grazing.}, number={1}, journal={JOURNAL OF ENVIRONMENTAL QUALITY}, author={Butler, David M. and Ranells, Noah N. and Franklin, Dorcas H. and Poore, Matthew H. and Green, James T., Jr.}, year={2007}, pages={155–162} }
 @article{bush_poore_rogers_altier_2007, title={Effect of stacking method on Salmonella elimination from recycled poultry bedding}, volume={98}, ISSN={["0960-8524"]}, DOI={10.1016/j.biortech.2006.02.017}, abstractNote={Recycled poultry bedding (RPB) is a protein and mineral supplement for cattle. Concerns regarding this product have arisen because of the perceived risk of transmitting potentially pathogenic organisms to cattle. This study's primary objective was to assess survival of Salmonella in RPB stacked to a recommended height (2.13 m—DS-RPB), or a height of 0.76 m (SS-RPB). Dialysis bags containing RPB and Salmonella typhimurium were placed throughout stacks. Temperature was monitored daily using thermocouples attached to sample bags. After 21 days, sample bags were recovered. Ammonia analysis was performed from multiple sites in the stacks. Bag contents were cultured to determine viability of the salmonella inoculates. This trial demonstrated a wide variation of temperature within the stacks. Temperature near the edge of stacks changed with ambient temperature. Ammonia concentration in the RPB was highest at the top of the DS-RPB. Salmonella was eliminated in 98.7% of sites, with at least a 5-log reduction in the Salmonella organisms in sites where it was still viable.}, number={3}, journal={BIORESOURCE TECHNOLOGY}, author={Bush, Dawn J. and Poore, Matthew H. and Rogers, Glenn M. and Altier, Craig}, year={2007}, month={Feb}, pages={571–578} }
 @article{butler_franklin_ranells_poore_green_2006, title={Ground cover impacts on sediment and phosphorus export from manured riparian pasture}, volume={35}, DOI={10.2134/ieq2005.0351}, number={6}, journal={Journal of Environmental Quality}, author={Butler, D. M. and Franklin, D. H. and Ranells, N. N. and Poore, M. H. and Green, J. T.}, year={2006}, pages={2178–2185} }
 @article{poore_scott_green_2006, title={Performance of beef heifers grazing stockpiled fescue as influenced by supplemental whole cottonseed}, volume={84}, ISSN={["0021-8812"]}, DOI={10.2527/2006.8461613x}, abstractNote={The objectives of this study were to determine the composition of stockpiled fescue from December through February over 2 yr and to determine the performance of heifers grazing stockpiled fescue with or without supplemental whole cottonseed. In early December, 36 heifers (initial BW 277 +/- 0.70 kg, yr 1; 266 +/- 2.2 kg, yr 2; and initial BCS of 5.0 +/- 0.04) were assigned randomly to 6 groups. Each group was assigned randomly to a 2.4-ha tall fescue pasture (98% endophyte infected), which had received 76 kg of N/ha on September 1. Group was the experimental unit for all measures. Forage DM available during grazing (to ground level) averaged 3,913 and 5,370 kg/ha in yr 1 and 2, respectively. The pasture was strip-grazed for 83 d, with daily forage allocation. Three groups were fed whole cottonseed (0.90 kg of DM/heifer; 24.4% CP, DM basis) daily at 0.33% of BW, and a small amount of a corn-based concentrate (0.19 kg of DM/heifer) to assure complete cottonseed consumption. Nutritive value of forage (dry basis) was determined each week by sampling each pasture to the 5-cm target grazing height. Forage disappearance was estimated every 2 wk from pre- and postgraze forage mass. Forage CP was 16.8% in yr 1 and 12.6% in yr 2. In vitro true organic matter digestibility (IVTOMD) was 82.0 and 71.9%, and ADF was 25.9 and 30.7% in yr 1 and 2, respectively. Most indicators of forage quality declined slightly through the winter, although they recovered in late winter in yr 1. The proportion of fescue that was green declined (P < 0.05) from December (79% in yr 1 and 64% in yr 2) to February (62% in yr 1 and 52% in yr 2). Green tissue averaged 20.4 and 15.2% CP, 91 and 87% IVTOMD, and 22.1 and 23.3% ADF in yr 1 and 2, respectively. Brown tissue averaged 10.3 and 8.5% CP, 64 and 62% IVTOMD, and 35.7 and 37.4% ADF in yr 1 and 2, respectively. Shrunk ADG (0.46 vs. 0.56 kg/d in yr 1 and 0.23 vs. 46 kg/d in yr 2) and change in BCS (- 0.03 vs. 0.33 in yr 1 and 0.13 vs. 0.5 in yr 2) was greater (P < 0.05) for supplemented heifers. Supplemented heifers had greater serum urea nitrogen in yr 1 (9.5 vs. 10.5 mg/dL; P < 0.07) and yr 2 (7.2 vs. 8.6 mg/dL; P < 0.01). Forage disappearance was similar between supplemented and unsupplemented heifers (3.19 vs. 3.39 kg.heifer(-1).d(-1) in yr 1 and 4.14 vs. 4.17 kg.heifer(-1).d(-1) in yr 2, respectively). Heifers responded to supplementation, but performance was lower than expected based on forage nutrient content.}, number={6}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Poore, MH and Scott, ME and Green, JT}, year={2006}, month={Jun}, pages={1613–1625} }
 @article{bertone_green_washburn_poore_watson_2006, title={The contribution of tunneling dung beetles to pasture soil nutrition}, ISBN={1547-4631}, DOI={10.1094/fg-2006-0711-02-rs}, abstractNote={A highly quality printing of images such as photographs, designs, and the like, is effected without using special paper. The following is provided: a supply mechanism for supplying a recording medium; means for applying a surface modifier for coating the surface modifier on the surface modification area, which is the area to be the modified of the recording medium supplied with the aforementioned means for supplying; and means for drying for drying the surface modification area coated on the aforementioned surface modification area. An area can be printed on the surface modification area with high quality. As such, high quality printing is made possible with regular paper, without using special paper, as before.}, journal={Forage and Grazinglands}, author={Bertone, M. A. and Green, J. T. and Washburn, S. P. and Poore, M. H. and Watson, D. W.}, year={2006}, pages={1} }
 @article{vibart_washburn_fellner_poore_green_brownie_2007, title={Varying endophyte status and energy supplementation of fresh tall fescue in continuous culture}, volume={132}, ISSN={["0377-8401"]}, DOI={10.1016/j.anifeedsci.2006.03.002}, abstractNote={Eight dual-flow continuous culture vessels (700 ml) were used to compare in vitro effects of toxic, endophyte-infected (E+), endophyte-free (E−), and non-toxic, endophyte-infected (EN) Jesup tall fescue (vegetative stage) on ruminal fermentation at 4 levels (0, 150, 300, and 450 g kg−1 DM) of concentrate supplementation (ground corn) for a total of 12 experimental diets in a randomized incomplete block design with 2 replicates. Each culture vessel was offered a total of 15 g DM d−1. Forage was fed in four equal portions (fed at 03:00, 09:00, 15:00, and 21:00 h); and corn was fed in two equal portions (fed at 09:00 and 21:00 h). Headspace gas and liquid samples were analyzed for methane, ruminal culture pH, ammonia–N, and volatile fatty acid production. Ammonia–N output (g d−1) varied by grass; EN had lower values compared to those of E+ and E−. Increasing the level of grain linearly decreased ruminal culture pH, ammonia–N, acetate production, and the acetate-to-propionate ratio, whereas propionate and butyrate production increased with higher grain supplementation. Ruminal fermentation was minimally altered by the presence of the endophyte; however, for the highest level of grain fed (450 g kg−1 DM fed) the methane production pattern for all three grasses was altered. In addition to having the lowest ruminal ammonia–N accumulation, the non-toxic, endophyte-infected fescue resulted in the lowest methane production measured.}, number={1-2}, journal={ANIMAL FEED SCIENCE AND TECHNOLOGY}, author={Vibart, R. E. and Washburn, S. P. and Fellner, V. and Poore, M. H. and Green, J. T., Jr. and Brownie, C.}, year={2007}, month={Jan}, pages={123–136} }
 @article{villarreal_cochran_rojas-bourrillon_murillo_munoz_poore_2006, title={Effect of supplementation with pelleted citrus pulp on digestibility and intake in beef cattle fed a tropical grass-based diet (Cynodon nlemfuensis)}, volume={125}, ISSN={["0377-8401"]}, DOI={10.1016/j.anifeedsci.2005.05.020}, abstractNote={Citrus pulp is an important by-product for sub-tropical and tropical ruminant animal production. In this study, three steers (average body weight = 324 ± 16 kg) were randomly assigned to three levels of pelleted citrus pulp (PCP) supplementation (0, 1.25, and 2.5 kg animal−1 d−1; as-fed) in a 3 × 3 Latin square design to evaluate its effects on forage intake, digestion, and ruminal pH. The basal diet was stargrass (Cynodon nlemfuensis) harvested and chopped every day and fed fresh. Supplementation with increasing amounts of PCP tended (P≤0.10) to result in a linear increase in digestibility of total diet dry matter (DM) and organic matter (OM), but no effects were noticed for digestibility of forage DM or total diet neutral detergent fiber. Forage DM intake decreased linearly (P=0.03) with increasing PCP supplementation, although the decrease tended (quadratic; P=0.08) to be of greater magnitude at the highest level of supplementation. Both a linear increase (P<0.01) and a quadratic trend (greatest increase with first level of supplementation; P=0.09) were also observed for intake of total digestible OM. Average ruminal pH was between 6.6 and 7.2 and was not affected (P=0.29) by supplementation treatment. Although supplementation with PCP depressed forage consumption somewhat, little effect on forage digestion was observed. The provision of digestible OM in the form of supplement was greater than that lost via depressed forage consumption, resulting in an overall increase in energy supply. Our results suggest that high levels of citrus pulp to beef cattle can lower forage intake, but increase total energy intake. High levels of citrus pulp supplementation could be beneficial in combination with forages high in rumen dagradable protein. Systems using grasses with higher ruminally degradable protein content than we used, may benefit from this extra supply of energy which should be tested in a further experiment.}, number={1-2}, journal={ANIMAL FEED SCIENCE AND TECHNOLOGY}, author={Villarreal, M and Cochran, RC and Rojas-Bourrillon, A and Murillo, O and Munoz, H and Poore, M}, year={2006}, month={Jan}, pages={163–173} }
 @article{matthews_poore_huntington_green_2005, title={Intake, digestion, and N metabolism in steers fed endophyte-free, ergot alkaloid-producing endophyte-infected, or nonergot alkaloid-producing endophyte-infected fescue hayI}, volume={83}, DOI={10.2527/2005.8351179x}, abstractNote={A digestion and N balance trial was conducted to compare effects of traditional endophyte-infected (E+), endophyte-free (E-), and nontoxic endophyte infected (NE; MaxQ; Pennington Seed, Inc., Madison, GA) Jesup tall fescue (Festuca arundinacea Schreb.) hay on digestion and N retention in steers. Hay composition (DM basis) was as follows: E+ (10.8% CP, 59.9% NDF, and 29.4% ADF), E- (11.8% CP, 58.5% NDF, and 28.4% ADF), and NE (11.6% CP, 58.6% NDF, and 28.3% ADF). Eight Polled Hereford steers (initial BW 240 +/- 9 kg) were used in a replicated, 3 x 3 Latin square design, with an extra steer allotted to each square. Steers were fed ad libitum for 14 d, followed by a 9-d adaptation to restricted intake (based on the animal with the lowest ad libitum intake for the square) and a 5-d fecal and urine collection. Water intake (20.2 L/d) and urine output (7.40 L/d) did not differ (P > 0.10) during the collection period. Plasma prolactin concentration was less (P < 0.05) for steers on the E+ hay (8.83 ng/mL) than for those on the E- hay (18.03 ng/mL) and intermediate for steers on the NE hay (12.65 ng/mL). Endophyte-infected hay differed (P < 0.05) from E- and NE in ad libitum DMI (5.02 vs. 5.62 and 5.61 kg/d, respectively) and ad libitum DMI as a percentage of BW (1.86 vs. 2.06 and 2.06%, respectively). Restricted DMI during the fecal and urine collection was lower (P < 0.05) for E+ hay than for E- (5.04 vs. 5.24 kg/d), and NE was intermediate (5.19 kg/d). Dry matter digestibility was lower (P < 0.05) for E+ compared with E- and NE (62.3 vs. 67.0 and 65.9%, respectively). Digestibility of ADF was lower (P < 0.05) for E+ than for E-, and was intermediate for NE (61.5, 66.0, and 63.9%, respectively). There were no differences for NDF, cellulose, or hemicellulose digestibilities among hay types. Crude protein digestibility was higher (P < 0.05) for E- and NE than for E+ (54.3 and 52.5 vs. 48.1%, respectively). Nitrogen retention was lower (P < 0.01) for E+ than for E- or NE (15.6 vs. 22.7 or 23.0 g/d, respectively). Hay type did not influence plasma urea N, urine urea N output, or urine urea N as a percentage of urinary N. Results from this study indicate that E+ tall fescue hay was lower in ad libitum DMI, DM digestibility, and N retention than NE or E- hays with similar chemical composition. Hay from NE and E- fescue had nearly identical composition, and did not differ for any variable measured.}, number={5}, journal={Journal of Animal Science}, author={Matthews, A. K. and Poore, M. H. and Huntington, G. B. and Green, J. T.}, year={2005}, pages={1179–1185} }
 @article{magee_poore_burns_huntington_2005, title={Nitrogen metabolism in beef steers fed gamagrass or orchardgrass hay with or without a supplement}, volume={85}, ISSN={["1918-1825"]}, DOI={10.4141/a04-045}, abstractNote={ A 50:50 soybean hull:corn grain supplement fed at 0.75% of body weight (BW) decreased ad libitum gamagrass or orchardgrass hay intake by steers, but increased total dry matter (DM) intake, DM digestibility, N intake, and N retained. Supplementation had a greater effect on N metabolism in steers fed orchardgrass than steers fed gamagrass. Key words: Steers, beef, hulls, soybean, metabolism, nitrogen }, number={1}, journal={CANADIAN JOURNAL OF ANIMAL SCIENCE}, author={Magee, KJ and Poore, MH and Burns, JC and Huntington, GB}, year={2005}, month={Mar}, pages={107–109} }
 @article{capucille_poore_rogers_2004, title={Growing and finishing performance of steers when fed recycled poultry bedding during the growing period}, volume={82}, DOI={10.2527/2004.82103038x}, abstractNote={Sixty Angus-cross steers were used to compare the effects of recycled poultry bedding (RPB) stacking method and the inclusion of monensin in growing diets on performance. Steers were individually fed balanced, growing diets for a period of 84 d. The diets were control (CON), CON + monensin (CON+M), deep-stacked RPB (DS), DS+M, shallow-stacked RPB (SS), and SS+M. The CON diets contained corn, soybean meal, corn silage, and cottonseed hulls. In the RPB diets, 35% of the silage, cottonseed hulls, and soybean meal was replaced with RPB (as-fed basis). At the end of the growing period, 30 steers, representing all treatment groups, had liver biopsies for trace mineral analysis and ruminal fluid samples to assess pH, VFA, and ammonia concentrations. All steers had blood samples drawn at the end of the growing period for analysis of Se and urea N. Steers were transported 466.6 km to simulate shipping stress and started on a finishing diet for a 120-d period. Intake, ADG, and G:F were monitored throughout the trial. Steers fed CON diets had higher ADG, DMI, and G:F than SS, and higher ADG and G:F than DS (P < 0.05) during the growing period. Steers fed DS diets had higher DMI than SS (P < 0.05) during the growing period. Inclusion of monensin in the growing diets increased G:F and decreased DMI (P < 0.05). Steers from the RPB treatments started the finishing period at lighter BW than steers fed CON diets (P < 0.05). During the finishing period, steers fed SS diets had higher DMI than steers fed CON diets (P < 0.06), whereas steers fed DS diets were intermediate. At slaughter, steers fed CON diets had higher hot carcass weights and quality grades than steers fed SS diets (P < 0.07), whereas steers fed DS diets were intermediate. Results indicate that steers fed RPB consumed it better when processed by deep stacking before consumption, that carryover effects of RPB into the finishing phase were minimal, and inclusion of monensin did not affect consumption of RPB diets.}, number={10}, journal={Journal of Animal Science}, author={Capucille, D. J. and Poore, M. H. and Rogers, G. M.}, year={2004}, pages={3038–3048} }
 @article{thibodeau_poore_hagler_rogers_2004, title={Effect of fermentation on sweetpotato (Ipomoea batatas) toxicity in mice}, volume={52}, ISSN={["1520-5118"]}, DOI={10.1021/jf034885k}, abstractNote={Unfortunate bovine fatalities occurring after ingestion of mold-damaged sweetpotatoes preclude the use of the culled tubers in livestock feed. In cattle, mold-damaged sweetpotatoes induce an acute respiratory distress syndrome resulting in asphyxiation. Because of this potential toxicity and the general abundance of culled sweetpotatoes, the detoxification efficacy of ensiling was explored since it is an easy and economically viable technique often applied to preserve livestock feed. Sweetpotato slices with or without mold damage were stored either frozen (to represent unfermented samples) or fermented for 6 weeks at room temperature. Following fermentation, organic extracts were generated for administration to mice. Thirty hours following administration of the extracts, mice were evaluated for gross and microscopic lesions affecting the lungs, liver, and kidneys. Fermentation of 6 weeks duration was observed to inadequately eliminate the lung, liver, and kidney toxicity caused by mold-damaged sweetpotatoes. In fact, fermentation exacerbated the hepatotoxicity of mold-damaged sweetpotatoes. This is also the first demonstration that sweetpotato regions lacking visible mold damage can induce lung and kidney injury, which, however, is preventable by fermentation.}, number={2}, journal={JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}, author={Thibodeau, MS and Poore, MH and Hagler, WM and Rogers, GM}, year={2004}, month={Jan}, pages={380–384} }
 @article{levy_gookin_poore_birkenheuer_dykstra_litaker_2003, title={Tritrichomonas foetus and not Pentatrichomonas hominis is the etiologic agent of feline trichomonal diarrhea}, volume={89}, ISSN={["1937-2345"]}, DOI={10.1645/0022-3395(2003)089[0099:TFANPH]2.0.CO;2}, abstractNote={Recently, several investigators have reported large-bowel diarrhea in cats associated with intestinal trichomonad parasites. These reports have presumptively identified the flagellates as Pentatrichomonas hominis, an organism putatively capable of infecting the intestinal tracts of a number of mammalian hosts, including cats, dogs, and man. The purpose of the present study was to determine the identity of this recently recognized flagellate by means of rRNA gene sequence analysis; restriction enzyme digest mapping; and light, transmission, and scanning electron microscopy (SEM).}, number={1}, journal={JOURNAL OF PARASITOLOGY}, author={Levy, MG and Gookin, JL and Poore, M and Birkenheuer, AJ and Dykstra, MJ and Litaker, RW}, year={2003}, month={Feb}, pages={99–104} }
 @article{gookin_foster_poore_stebbins_levy_2003, title={Use of a commercially available culture system for diagnosis of Tritrichomonas foetus infection in cats}, volume={222}, url={https://doi.org/10.2460/javma.2003.222.1376}, DOI={10.2460/javma.2003.222.1376}, abstractNote={AbstractObjective—To evaluate the efficacy of and optimize a commercially available culture system for sensitive and specific in-clinic culture ofTritrichomonas foetusfrom cat feces.Design—Prospective study.Sample Population—Samples of freshly voided feces from 117 purebred cats and pure cultures ofT foetusobtained from a cat with chronic diarrhea.Procedure—Optimal conditions for use of the culture system, such as quantity of fecal inoculum (0.025 to 0.2 g) and cultivation temperature (25 or 37°C [98.6 or 77.0°F]), were determined. Specificity of the system was examined by attempted culture ofGiardia lambliaandPentatrichomonas hominis. Sensitivity of the system to detectT foetuswas determined by inoculation of culture system pouches with serially dilutedT foetussuspensions with and without feces.Results—Detection limit of the culture system was 1 and 1,000T foetusorganisms without and with feces from cats, respectively. Optimal fecal inoculum was < 0.1 g of feces. At 37°C, cultures yielded positive results in 24 hours; organisms remained viable for 1 to 6 days, and bacterial overgrowth was common. At 25°C, cultures yielded positive results in 1 to 11 days; organisms were long-lived, and bacterial overgrowth was uncommon. NeitherG lambliaorP hominissurvived in the culture system.Conclusions and Clinical Relevance—The culture system was sensitive and specific for culture ofT foetusin feces of cats. Performance was optimal when test kits were inoculated with ≤ 0.1 g of freshly voided feces and cultured at 25°C. (J Am Vet Med Assoc2003;222:1376–1379)}, number={10}, journal={JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION}, author={Gookin, JL and Foster, DM and Poore, MF and Stebbins, ME and Levy, MG}, year={2003}, month={May}, pages={1376–1379} }
 @article{rogers_poore_2002, title={Alternative feeds for beef cattle}, volume={18}, ISSN={["0749-0720"]}, DOI={10.1016/S0749-0720(02)00024-5}, number={2}, journal={VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE}, author={Rogers, GM and Poore, MH}, year={2002}, month={Jul}, pages={XI-XII} }
 @article{moore_poore_luginbuhl_2002, title={By-product feeds for meat goats: Effects on digestibility, ruminal environment, and carcass characteristics}, volume={80}, DOI={10.2527/2002.8071752x}, abstractNote={Crossbred wether goats (n = 24; 50% Boer, 6 per diet) initially averaging 27.4+/-0.4 kg were fed either wheat middlings (wheat midds), soybean hulls (soyhulls), or corn gluten feed at 1% BW (as-fed) along with orchardgrass hay (10.7% CP) offered to ad-libitum consumption for 72 d followed by 5 d total fecal collection. The Control (hay) diet was supplemented with 5.7% soybean meal to bring total dietary protein to 12.5%, by-products were brought to a higher Ca:P ratio with limestone or dicalcium phosphate to make total dietary Ca:P 1.5:1, and soybean meal was added to soyhulls to bring them up to 17% CP (wheat midds = 17% and corn gluten feed = 21% CP). Total DMI (916 g/d+/-57 or 3.2%+/-0.2 BW) did not differ (P > 0.92) among treatments. Initial BW (P = 0.25), final BW (P = 0.48), and ADG (P = 0.56) did not differ for the four treatments. Carcass weight was greater (P = 0.05) for goats fed soyhulls (16.0 kg) or wheat midds (15.6 kg) as compared with goats fed the hay diet (14.5 kg), with carcass weight from goats fed corn gluten feed being intermediate (15.3 kg, SEM = 0.3 kg). Carcass grade did not differ (P = 0.80) and averaged 5.42+/-0.4. Dressing percentage tended (P = 0.12) to be lower for goats fed the hay diet (46.4%) compared with soyhull (48.3%), corn gluten feed (48.3%), or wheat midd (48.8%) diets (SEM = 0.7). Ruminal pH was highest (P < 0.01) for goats fed the hay diet (6.52) and lowest for goats fed wheat midds (6.23) with soyhull (6.41) and corn gluten feed diets (6.35) being intermediate (SEM = 0.05). Digestibility of DM (70.1+/-2.5%), OM (70.3+/-2.6%,), CP (75.5+/-2.0%), GE (68.5+/-2.7%), NDF (68.1+/-3.0%), ADF (65.4+/-3.4%), cellulose (70.1+/-2.9%), and lignin (31.1+/-8.2%) did not differ (P > 0.15). Total ruminal VFA did not differ (86.0+/-6.1 mM, P = 0.59), but acetate:propionate ratio was higher (P < 0.01) for hay (3.1) and soyhull diets (3.3) than for corn gluten feed (2.4) and wheat midd diets (2.4, SEM = 0.11). Ruminal ammonia (mg/100 mL) was lower (P < 0.01) for goats fed hay (15.4) and soyhull diets (11.6) than those fed corn gluten feed (25.2) and wheat midd diets (23.0, SEM = 1.35). Ruminal pH was lower for goats fed the byproducts, but remained above 6. Serum urea nitrogen (mg/100 mL) averaged 21.0+/-1.0 (P = 0.11) with soyhulls tending to be lowest (19.3) and corn gluten feed tending to be highest (22.8). Soyhulls, corn gluten feed, and wheat midds appear to be viable feed ingredients for meat goat diets.}, number={7}, journal={Journal of Animal Science}, author={Moore, J. A. and Poore, M. H. and Luginbuhl, J. M.}, year={2002}, pages={1752–1758} }
 @article{poore_2002, title={Dedication - Bill Kunkle (1947-2002)}, volume={18}, ISSN={["0749-0720"]}, DOI={10.1016/s0749-0720(02)00025-7}, number={2}, journal={VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE}, author={Poore, MH}, year={2002}, month={Jul}, pages={XIII-XIII} }
 @article{capucille_poore_altier_rogers_2002, title={Evaluation of Salmonella shedding in cattle fed recycled poultry bedding}, volume={36}, ISBN={0524-1685}, number={1}, journal={Bovine Practitioner}, author={Capucille, D. J. and Poore, M. H. and Altier, C. and Rogers, G. M.}, year={2002}, pages={15} }
 @article{rogers_poore_paschal_2002, title={Feeding cotton products to cattle}, volume={18}, ISSN={["1558-4240"]}, DOI={10.1016/S0749-0720(02)00020-8}, abstractNote={Despite the potential for gossypol toxicosis (particularly in pre-ruminants) and risk factors associated with impaired fertility in bulls, cottonseed products offer a safe alternative feed for cattle producers when fed at recommended levels. Beef producers seeking to lower production costs should consider using cotton byproducts in their feeding programs. If carefully incorporated, cotton byproduct feeds can reduce feed costs while maintaining or increasing the level of cattle performance. Cottonseed meal will remain a standard protein supplement for beef cattle throughout the country. Whole cottonseed has much potential for Southern producers near cotton gins if it is purchased in a timely fashion and fed according to recommendations. Cotton gin trash, cottonseed hulls, and cotton textile mill waste also have potential economic benefits, especially to producers located near cotton and cottonseed processing facilities.}, number={2}, journal={VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE}, author={Rogers, GM and Poore, MH and Paschal, JC}, year={2002}, month={Jul}, pages={267-+} }
 @article{thibodeau_poore_rogers_2002, title={Health and production aspects of feeding sweetpotato to cattle}, volume={18}, ISSN={["1558-4240"]}, DOI={10.1016/S0749-0720(02)00022-1}, abstractNote={If certain guidelines are followed when feeding sweetpotatoes to livestock it is possible to minimize health hazards. Careful herd management and the recognition of specific biomarkers such as excessive dental deterioration could aid in the early identification of feed problems. Where these tubers are produced locally in abundance there can be an economic and environmental incentive to divert waste sweetpotato by-products toward livestock feed. The feeding of culled sweetpotatoes and processed sweetpotato waste by-products can have three major benefits. First, expensive disposal costs are reduced. Second, negative environmental impacts from landfill dumping and crop spreading are limited. Third, the culled sweetpotatoes and SPCW offer an inexpensive and nutritious alternative feed ration for livestock that may increase economic returns.}, number={2}, journal={VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE}, author={Thibodeau, MS and Poore, MH and Rogers, GM}, year={2002}, month={Jul}, pages={349-+} }
 @article{watson_stringham_denning_washburn_poore_meier_2002, title={Managing the horn fly (Diptera : Muscidae) using an electric walk-through fly trap}, volume={95}, ISSN={["1938-291X"]}, DOI={10.1603/0022-0493-95.5.1113}, abstractNote={An electric walk-through ßy trap was evaluated for the management of the horn ßy, Hematobia irritans (L.), on dairy cattle in North Carolina over 2 yr. The trap relies on black lights and electrocution grids to attract and kill ßies that are brushed from the cattle passing through. During the Þrst season, horn ßy densities were reduced from 1,400 to 200 ßies per animal. Horn ßy density averaged 269.2 25.8 on cattle using the walk-through ßy trap twice daily, and 400.2 43.5 on the control group during the Þrst year. The second year, seasonal mean horn ßy density was 177.3 10.8 on cattle using the walk-through ßy trap compared with 321.1 15.8 on the control group. No insecticides were used to control horn ßies during this 2-yr study.}, number={5}, journal={JOURNAL OF ECONOMIC ENTOMOLOGY}, author={Watson, DW and Stringham, SM and Denning, SS and Washburn, SP and Poore, MH and Meier, A}, year={2002}, month={Oct}, pages={1113-+} }
 @article{rankins_poore_capucille_rogers_2002, title={Recycled poultry bedding as cattle feed}, volume={18}, DOI={10.1016/S0749-0720(02)00015-4}, abstractNote={Since the 1950s, recycled poultry bedding has been used as an economical feedstuff for beef cattle. It has been extensively studied at several experiment stations around the world with regard to its safety and nutritional aspects. It will continue to be closely scrutinized as the public increases its awareness of agricultural issues. As this study was being prepared, the news media was "spotlighting" bovine spongiform encephalopathy. Currently, in the United States there is a ban on incorporation of mammalian-derived protein feeds into ruminant diets. This has led to a requirement of beef cattle producers signing affidavits indicating that they had met this obligation. Some poultry companies use ruminant meat and bone meal in broiler diets when least-cost formulation indicates that it is economically desirable. This then poses the question of whether feeding RPB to beef cattle should be permitted if the birds had been fed ruminant meat and bone meal. It also raises the question of whether cattle grazing pastures fertilized with RPB are exposed to ruminant meat and bone meal. Because of the importance of pasture fertilization as a waste disposal solution for the poultry industry, it seems that the issue will be quickly resolved by omitting the ruminant meat and bone meal from poultry diets should concerns increase. Use of RPB, like many byproduct feeds, requires a higher level of management expertise than traditional feeds. Despite the potential problems discussed in this study, an informed beef cattle producer can gain a financially competitive edge by using RPB. A simple processing method, deep-stacking under polyethylene sheeting, can produce a safe product that will provide a complete diet when blended with an energy source and supplemented with some long-stem fiber. The diets can be used for both brood cows and stocker calves for extended periods of time, and the practice of feeding RPB is safe for both cattle and consumers [45]. Economic parameters will influence the future use of RPB; however, the general public's perception and acceptance will ultimately determine its long-term use.}, number={2}, journal={Veterinary Clinics of North America. Food Animal Practice}, author={Rankins, D. L. and Poore, M. H. and Capucille, D. J. and Rogers, G. M.}, year={2002}, pages={253-} }
 @article{poore_johns_burris_2002, title={Soybean hulls, wheat middlings, and corn gluten feed as supplements for cattle on forage-based diets}, volume={18}, ISSN={["0749-0720"]}, DOI={10.1016/S0749-0720(02)00021-X}, abstractNote={Soybean hulls, wheat midds, and corn gluten feed are viable alternative supplements for forage-fed cattle. All three result from the processing of major Unites States agricultural crops, so large supplies are available. Their value is better for ruminant animals than for monogastrics because they contain digestible fiber components. These byproducts are widely available throughout the Unites States and will generally be more economical than traditional feed grains or commercial feeds when used appropriately as supplements to forage-based diets. Knowledge about the composition of base forage must be used in planning supplementation strategies because base forages vary in protein and mineral content [9]. Soybean hulls alone may be a good selection in situations in which forages are adequate or high in protein. In situations where forage is marginal or deficient in protein, wheat midds, corn gluten feed, or a mix of soybean hulls and corn gluten feed might be most desirable. All three feeds can be variable in nutrient composition, so they should be analyzed to ensure a balanced nutrient level in diets. Soybean hulls are especially variable in crude protein content and should always be analyzed when forages are marginal or deficient in protein. Despite the fact that published energy levels are substantially lower, research has shown that soybean hulls and wheat midds have a value comparable to corn and soybean meal in forage-based diets. Corn gluten feed has also been comparable to corn and soybean meal in most reports but is closer in value to its published energy levels. In general, results with soybean hulls have been surprisingly good and consistent, whereas responses to wheat midds and corn gluten feed supplementation have been more variable and sometimes disappointing. Feeding rates for soybean hulls can range from low to extremely high depending on forage availability and desired performance. Wheat midds should be limited in most situations to 50% of the expected dry matter intake because of their rapidly digested starch content. Corn gluten feed should be limited to approximately 50% of expected dry matter intake because of its high sulfur content. Several research groups are currently evaluating self-feeding programs for these byproducts, and while the potential for health and production problems exist, results have been encouraging in most cases. Self-feeding would reduce the labor costs of hand feeding and could provide backgrounders with a program to expand cattle numbers on a limited land base.}, number={2}, journal={VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE}, author={Poore, MH and Johns, JT and Burris, WR}, year={2002}, month={Jul}, pages={213-+} }
 @article{hopkins_poore_2001, title={Deep-stacked broiler litter as a protein supplement for dairy replacement heifers}, volume={84}, ISSN={["0022-0302"]}, DOI={10.3168/jds.S0022-0302(01)74480-3}, abstractNote={Fifty Holstein dairy heifers, [two replications of 25 heifers; average body weight (BW) 227 kg], were assigned to one of the following treatments for 105 d. Treatments 1 through 4 are given as the percentage of supplemental crude protein (CP) from soybean meal and deep-stacked broiler litter, respectively: 1) 100 and 0%; 2) 67 and 33%; 3) 33 and 67%; 4) 0 and 100%; and 5) 67% of supplementary CP from deep-stacked broiler litter and 33% from a source of rumen undegradable protein (RUP). All rations were formulated to contain 15% CP and 68% TDN on a dry matter basis. Dry matter intake was not significantly different among treatments at 10.1, 10.2, 10.4, 9.4, and 9.1 kg/d for treatments 1 through 5, respectively. Average daily BW gains (1.35, 1.37, 1.28, 1.17, and 1.24 kg/d for treatments 1 through 5, respectively) decreased linearly as the level of litter feeding increased and was not influenced by increasing RUP in treatment 5. Feed efficiency was not affected by the level of deep-stacked broiler litter feeding. Ruminal ammonia and plasma urea nitrogen concentrations decreased linearly as the level of litter feeding increased. Feeding additional RUP decreased ruminal ammonia concentration in treatment 5 to a level similar to treatment 4. This suggests that the protein in deep-stacked broiler litter may be more undegradable than that in soybean meal and that RUP in broiler litter may be higher than previously thought. Results from this study indicate that deep-stacked broiler litter has potential as a low-cost CP source for growing dairy heifers.}, number={1}, journal={JOURNAL OF DAIRY SCIENCE}, author={Hopkins, BA and Poore, MH}, year={2001}, month={Jan}, pages={299–305} }
 @article{huntington_poore_hopkins_spears_2001, title={Effect of ruminal protein degradability on growth and N metabolism in growing beef steers}, volume={79}, DOI={10.2527/2001.792533x}, abstractNote={The objective of two experiments was to correlate plasma levels of urea N (PUN) and the percentage of urine N in the form of urea (UUN) to weight gain in response to different dietary protein regimens for growing Angus steers. In Exp. 1, 60 steers (302 kg BW) were assigned to various levels of dietary N (control plus supplemental N to provide from 100 to 400 g more crude protein daily) within two sources of supplemental N (soybean meal [SBM] or a mixture of two parts corn gluten meal:one part blood meal [CGM:BM]). In Exp. 2, 27 steers (229 kg BW) were fed two levels of SBM, and half of the steers received growth-promoting implants. Steers were housed in groups of 12 and fed individually for 84 d in both experiments. Corn silage was fed at a restricted rate to minimize orts. Jugular blood and urine samples were collected during the experiments. In Exp. 1, maximal ADG of steers fed SBM (1.0 kg) was reached with 671 g/d total crude protein, or 531 g/d metabolizable protein. Maximal ADG of steers fed CGM:BM (0.91 kg) was reached with 589 g/d total crude protein, or 539 g/d metabolizable protein. The DMI was higher (P < 0.07) for steers fed SBM (6.37 kg/d) than for steers fed CGM:BM (6.14 kg/d). Increasing ruminal escape protein from 36% (SBM) to 65% (CGM:BM) of CP decreased (P < 0.05) endogenous production of urea, as evidenced by lower concentrations of urea in blood and lower UUN. In Exp. 2, increasing supplemental protein from 100 to 200 g/d increased (P < 0.05) ADG and PUN. Implants lowered (P < 0.05) UUN, particularly at the higher level of supplemental protein. Protein supplementation of growing steers can be managed to maintain acceptable ADG yet decrease excretion of urea in the urine.}, number={2}, journal={Journal of Animal Science}, author={Huntington, G. and Poore, M. and Hopkins, B. and Spears, J.}, year={2001}, pages={533–541} }
 @article{gookin_levy_law_papich_poore_breitschwerdt_2001, title={Experimental infection of cats with Tritrichomonas foetus}, volume={62}, ISSN={0002-9645}, url={http://dx.doi.org/10.2460/ajvr.2001.62.1690}, DOI={10.2460/ajvr.2001.62.1690}, abstractNote={AbstractObjective—To determine whether infection withTritrichomonas foetuscauses diarrhea in specific pathogen-free orCryptosporidiumcoinfected cats.Animals—4 cats with subclinical cryptosporidiosis (group 1) and 4 specific-pathogen-free cats (group 2).Procedure—Cats were infected orogastrically with an axenic culture ofT foetusisolated from a kitten with diarrhea. Direct microscopy and protozoal culture of feces, fecal character, serial colonic mucosal biopsy specimens, and response to treatment with nitazoxanide (NTZ; group 1) or prednisolone (groups 1 and 2) were assessed.Results—Infection withT foetuspersisted in all cats for the entire 203-day study and resulted in diarrhea that resolved after 7 weeks. Group-1 cats had an earlier onset, more severe diarrhea, and increased number of trichomonads on direct fecal examination, compared with group-2 cats. Use of NTZ eliminated shedding ofT foetusandCryptosporidiumoocysts, but diarrhea consisting of trichomonad-containing feces recurred when treatment was discontinued. Prednisolone did not have an effect on infection withT foetusbut resulted in reappearance ofCryptosporidiumoocysts in the feces of 2 of 4 cats. During necropsy,T foetuswas isolated from contents of the ileum, cecum, and colon.Tritrichomonas foetusorganisms and antigen were detected on surface epithelia and within superficial detritus of the cecal and colonic mucosa.Conclusions and Clinical Relevance—After experimental inoculation in cats,T foetusorganisms colonize the ileum, cecum, and colon, reside in close contact with the epithelium, and are associated with transient diarrhea that is exacerbated by coexisting cryptosporidiosis but not treatment with prednisolone. (Am J Vet Res2001;62:1690–1697)}, number={11}, journal={American Journal of Veterinary Research}, publisher={American Veterinary Medical Association (AVMA)}, author={Gookin, Jody L. and Levy, Michael G. and Law, J. Mac and Papich, Mark G. and Poore, Matthew F. and Breitschwerdt, Edward B.}, year={2001}, month={Nov}, pages={1690–1697} }
 @article{luginbuhl_poore_spears_brown_2000, title={Effect of dietary copper level on performance and copper status of growing meat goats}, volume={16}, number={2000}, journal={Sheep & Goat Research Journal}, author={Luginbuhl, J. M. and Poore, M. H. and Spears, J. W. and Brown, T. T.}, year={2000}, pages={65–71} }
 @article{luginbuhl_poore_conrad_2000, title={Effect of level of whole cottonseed on intake, digestibility, and performance of growing male goats fed hay-based diets}, volume={78}, DOI={10.2527/2000.7861677x}, abstractNote={Twenty-four purebred Boer (Capra hircus hircus) and 12 male kids of 1/2 Boer breeding (initial BW 21 +/- .5 kg) were used in a randomized complete block design to study the effects of increasing level of whole cottonseed (WCS) on ADG, serum urea N, plasma gossypol, live grades, and intake of DM, CP, NDF, ADF, hemicellulose, cellulose, lignin, ether extract, and free gossypol. A subgroup of 16 purebred goats was used to determine digestibility using a 5-d total fecal collection. Goats were blocked by BW and then assigned at random to one of four diets containing 0, 8, 16, or 24% WCS. All diets contained 71% chopped orchardgrass (Dactylis glomerata L.) hay and 29% concentrate; WCS replaced corn and soybean meal to maintain calculated TDN and CP concentrations at 68 and 15% of DM, respectively. Concentrations of NDF in diet DM were 52.4, 55.9, 59.3, and 62.1% with increasing WCS, and the Ca:P ratio was maintained at 2:0. Goats were given ad libitum access to feed and water. Over the 90-d performance phase, DMI (P < .05), CP intake (P < .02), and ADG (P < .01) decreased linearly with increasing WCS in the diet, whereas ether extract (EE) intake increased in a cubic fashion (P < .01). Gain:feed decreased linearly (P < .02) with increasing level of WCS. Addition of WCS resulted in linear decreases in apparent digestibility coefficients of DM (P < .02) and NDF (P < .05), a linear increase in total plasma gossypol (P < .01), and a quadratic increase in serum urea N (P < .04). Apparent digestibility of CP was not affected by WCS level. At the 16 and 24% WCS levels, EE constituted 4.2 and 4.8% of total DMI, respectively. Adding WCS to diets for growing goats had detrimental effects on animal performance, and, based on the possible negative effects of dietary EE and NDF rather than gossypol, economics should dictate whether to use WCS in feeding programs.}, number={6}, journal={Journal of Animal Science}, author={Luginbuhl, J. M. and Poore, M. H. and Conrad, A. P.}, year={2000}, pages={1677–1683} }
 @article{use of goats to manage vegetation in cattle pastures in the appalachian region of north carolina_2000, volume={16}, number={2000}, journal={Sheep & Goat Research Journal}, year={2000}, pages={124–135} }
 @article{mueller_poore_skroch_1999, title={Damage assessment in Christmas tree plantations following vegetation control with sheep and geese}, volume={23}, number={1}, journal={Southern Journal of Applied Forestry}, author={Mueller, J.P. and Poore, M.H. and Skroch, W.A.}, year={1999}, pages={11–15} }
 @article{rogers_poore_ferko_brown_deaton_bawden_1999, title={Dental wear and growth performance in steers fed sweetpotato cannery waste}, volume={214}, number={5}, journal={Journal of the American Veterinary Medical Association}, author={Rogers, G. M. and Poore, M. H. and Ferko, B. L. and Brown, T. T. and Deaton, T. G. and Bawden, J. W.}, year={1999}, pages={681–687} }
 @article{ferko_poore_schultheis_rogers_1998, title={Feeding potato and sweetpotato byproducts to beef cattle}, volume={93}, number={1}, journal={Veterinary Medicine}, author={Ferko, B. L. and Poore, M. H. and Schultheis, J. R. and Rogers, G. M.}, year={1998}, pages={82-} }
 @article{luginbuhl_harvey_green_poore_mueller_1998, title={Use of goats as biological agents for the renovation of pastures in the Appalachian region of the United States}, volume={44}, ISSN={["0167-4366"]}, DOI={10.1023/A:1006250728166}, number={2-3}, journal={AGROFORESTRY SYSTEMS}, author={Luginbuhl, JM and Harvey, TE and Green, JT and Poore, MH and Mueller, JP}, year={1998}, pages={241–252} }
 @article{rogers_poore_1997, title={Dental effects of feeding sweet potato cannery waste in beef cattle}, volume={19}, number={1, suppl.}, journal={Compendium on Continuing Education for the Practicing Veterinarian}, author={Rogers, G. M. and Poore, M. H.}, year={1997}, pages={S41–46} }
 @article{rogers_poore_ferko_kusy_deaton_bawden_1997, title={In vitro effects of an acidic by product feed on bovine teeth}, volume={58}, number={5}, journal={American Journal of Veterinary Research}, author={Rogers, G. M. and Poore, M. H. and Ferko, B. L. and Kusy, R. P. and Deaton, T. G. and Bawden, J. W.}, year={1997}, pages={498–503} }
 @article{poore_rogers_1995, title={Feeding whole cottonseed and other cotton by-products to beef cattle}, volume={90}, number={11}, journal={Veterinary Medicine}, author={Poore, M. H. and Rogers, G.}, year={1995}, pages={1077} }